LOCUS       DACEMS010000001        74503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEMS010000001 DACEMS010000000
VERSION     DACEMS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.001"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(159..1784)
                     /gene="groL"
                     /locus_tag="IW735_000001"
     CDS             complement(159..1784)
                     /gene="groL"
                     /locus_tag="IW735_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1043036.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(1835..2119)
                     /locus_tag="IW735_000002"
     CDS             complement(1835..2119)
                     /locus_tag="IW735_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1043037.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            2344..3006
                     /locus_tag="IW735_000003"
     CDS             2344..3006
                     /locus_tag="IW735_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1043038.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(3082..4374)
                     /locus_tag="IW735_000004"
     CDS             complement(3082..4374)
                     /locus_tag="IW735_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1043039.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            4544..5173
                     /locus_tag="IW735_000005"
     CDS             4544..5173
                     /locus_tag="IW735_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ1043040.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(5276..6085)
                     /locus_tag="IW735_000006"
     CDS             complement(5276..6085)
                     /locus_tag="IW735_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1043041.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(6140..7009)
                     /locus_tag="IW735_000007"
     CDS             complement(6140..7009)
                     /locus_tag="IW735_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043042.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(7010..8326)
                     /locus_tag="IW735_000008"
     CDS             complement(7010..8326)
                     /locus_tag="IW735_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043043.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(8323..10158)
                     /gene="walK"
                     /locus_tag="IW735_000009"
     CDS             complement(8323..10158)
                     /gene="walK"
                     /locus_tag="IW735_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1043044.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(10163..10867)
                     /locus_tag="IW735_000010"
     CDS             complement(10163..10867)
                     /locus_tag="IW735_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1043045.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(11057..11917)
                     /locus_tag="IW735_000011"
     CDS             complement(11057..11917)
                     /locus_tag="IW735_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1043046.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(11904..12473)
                     /locus_tag="IW735_000012"
     CDS             complement(11904..12473)
                     /locus_tag="IW735_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1043047.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            12854..12926
                     /locus_tag="IW735_000013"
     tRNA            12854..12926
                     /locus_tag="IW735_000013"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12887..12889,aa:Lys,seq:ctt)
     gene            complement(13422..13808)
                     /locus_tag="IW735_000014"
     CDS             complement(13422..13808)
                     /locus_tag="IW735_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1043048.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(13805..14326)
                     /locus_tag="IW735_000015"
     CDS             complement(13805..14326)
                     /locus_tag="IW735_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1043049.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(14328..15362)
                     /gene="sppA"
                     /locus_tag="IW735_000016"
     CDS             complement(14328..15362)
                     /gene="sppA"
                     /locus_tag="IW735_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1043050.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(15395..16810)
                     /locus_tag="IW735_000017"
     CDS             complement(15395..16810)
                     /locus_tag="IW735_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ1043051.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(16779..18932)
                     /locus_tag="IW735_000018"
     CDS             complement(16779..18932)
                     /locus_tag="IW735_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ1043052.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            19190..19426
                     /locus_tag="IW735_000019"
     CDS             19190..19426
                     /locus_tag="IW735_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1043053.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            19445..19660
                     /locus_tag="IW735_000020"
     CDS             19445..19660
                     /locus_tag="IW735_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1043054.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(20363..21052)
                     /locus_tag="IW735_000021"
     CDS             complement(20363..21052)
                     /locus_tag="IW735_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1043055.1"
                     /translation="MVLIKSMNYSGFKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDL
                     NHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNE
                     IVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDG
                     QYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQ
                     KIKEKLKESTV"
     gene            21176..22495
                     /locus_tag="IW735_000022"
     CDS             21176..22495
                     /locus_tag="IW735_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ1043056.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDKRAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(22701..22799)
                     /locus_tag="IW735_000023"
                     /pseudo
     CDS             complement(22701..22799)
                     /locus_tag="IW735_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            complement(22814..24061)
                     /locus_tag="IW735_000024"
     CDS             complement(22814..24061)
                     /locus_tag="IW735_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1043057.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(24137..24283)
                     /locus_tag="IW735_000025"
     CDS             complement(24137..24283)
                     /locus_tag="IW735_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ1043058.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(24387..24698)
                     /locus_tag="IW735_000026"
     CDS             complement(24387..24698)
                     /locus_tag="IW735_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1043059.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(24700..24897)
                     /locus_tag="IW735_000027"
     CDS             complement(24700..24897)
                     /locus_tag="IW735_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1043060.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(25291..27018)
                     /locus_tag="IW735_000028"
     CDS             complement(25291..27018)
                     /locus_tag="IW735_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1043061.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(27011..28204)
                     /locus_tag="IW735_000029"
     CDS             complement(27011..28204)
                     /locus_tag="IW735_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1043062.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(28391..29035)
                     /locus_tag="IW735_000030"
     CDS             complement(28391..29035)
                     /locus_tag="IW735_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1043063.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(29129..29263)
                     /locus_tag="IW735_000031"
     CDS             complement(29129..29263)
                     /locus_tag="IW735_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1043064.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(29265..31397)
                     /gene="feoB"
                     /locus_tag="IW735_000032"
     CDS             complement(29265..31397)
                     /gene="feoB"
                     /locus_tag="IW735_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1043065.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(31394..31867)
                     /locus_tag="IW735_000033"
     CDS             complement(31394..31867)
                     /locus_tag="IW735_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1043066.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            32268..32492
                     /gene="nrdH"
                     /locus_tag="IW735_000034"
     CDS             32268..32492
                     /gene="nrdH"
                     /locus_tag="IW735_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1043067.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            32651..34810
                     /gene="nrdE"
                     /locus_tag="IW735_000035"
     CDS             32651..34810
                     /gene="nrdE"
                     /locus_tag="IW735_000035"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1043068.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            34860..35825
                     /gene="nrdF"
                     /locus_tag="IW735_000036"
     CDS             34860..35825
                     /gene="nrdF"
                     /locus_tag="IW735_000036"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1043069.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(35914..37053)
                     /locus_tag="IW735_000037"
     CDS             complement(35914..37053)
                     /locus_tag="IW735_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1043070.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(37362..38075)
                     /locus_tag="IW735_000038"
     CDS             complement(37362..38075)
                     /locus_tag="IW735_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1043071.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(38329..39030)
                     /gene="nagB"
                     /locus_tag="IW735_000039"
     CDS             complement(38329..39030)
                     /gene="nagB"
                     /locus_tag="IW735_000039"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1043072.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            39264..40796
                     /locus_tag="IW735_000040"
     CDS             39264..40796
                     /locus_tag="IW735_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1043073.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQINWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(40987..42189)
                     /locus_tag="IW735_000041"
     CDS             complement(40987..42189)
                     /locus_tag="IW735_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1043074.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(42271..42960)
                     /locus_tag="IW735_000042"
     CDS             complement(42271..42960)
                     /locus_tag="IW735_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1043075.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(43066..43434)
                     /gene="rplL"
                     /locus_tag="IW735_000043"
     CDS             complement(43066..43434)
                     /gene="rplL"
                     /locus_tag="IW735_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1043076.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(43487..43990)
                     /locus_tag="IW735_000044"
     CDS             complement(43487..43990)
                     /locus_tag="IW735_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1043077.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(44400..45089)
                     /gene="rplA"
                     /locus_tag="IW735_000045"
     CDS             complement(44400..45089)
                     /gene="rplA"
                     /locus_tag="IW735_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1043078.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(45193..45615)
                     /gene="rplK"
                     /locus_tag="IW735_000046"
     CDS             complement(45193..45615)
                     /gene="rplK"
                     /locus_tag="IW735_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1043079.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            45958..46626
                     /gene="sdaAB"
                     /locus_tag="IW735_000047"
     CDS             45958..46626
                     /gene="sdaAB"
                     /locus_tag="IW735_000047"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1043080.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            46639..47511
                     /gene="sdaAA"
                     /locus_tag="IW735_000048"
     CDS             46639..47511
                     /gene="sdaAA"
                     /locus_tag="IW735_000048"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1043081.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(47602..48621)
                     /locus_tag="IW735_000049"
     CDS             complement(47602..48621)
                     /locus_tag="IW735_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1043082.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(48794..49681)
                     /locus_tag="IW735_000050"
     CDS             complement(48794..49681)
                     /locus_tag="IW735_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1043083.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(49758..50303)
                     /gene="nusG"
                     /locus_tag="IW735_000051"
     CDS             complement(49758..50303)
                     /gene="nusG"
                     /locus_tag="IW735_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1043084.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(50404..50574)
                     /gene="secE"
                     /locus_tag="IW735_000052"
     CDS             complement(50404..50574)
                     /gene="secE"
                     /locus_tag="IW735_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1043085.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(50598..50750)
                     /gene="rpmG"
                     /locus_tag="IW735_000053"
     CDS             complement(50598..50750)
                     /gene="rpmG"
                     /locus_tag="IW735_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1043086.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            50912..51556
                     /locus_tag="IW735_000054"
     CDS             50912..51556
                     /locus_tag="IW735_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1043087.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(51612..52115)
                     /locus_tag="IW735_000055"
     CDS             complement(51612..52115)
                     /locus_tag="IW735_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1043088.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(52128..52174)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            52712..53677
                     /locus_tag="IW735_000056"
     CDS             52712..53677
                     /locus_tag="IW735_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1043089.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(53776..54948)
                     /locus_tag="IW735_000057"
     CDS             complement(53776..54948)
                     /locus_tag="IW735_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1043090.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(54935..56080)
                     /locus_tag="IW735_000058"
     CDS             complement(54935..56080)
                     /locus_tag="IW735_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1043091.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(56094..57018)
                     /locus_tag="IW735_000059"
                     /pseudo
     CDS             complement(56094..57018)
                     /locus_tag="IW735_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            57135..58214
                     /locus_tag="IW735_000060"
     CDS             57135..58214
                     /locus_tag="IW735_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1043092.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            58246..58839
                     /locus_tag="IW735_000061"
     CDS             58246..58839
                     /locus_tag="IW735_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1043093.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(58949..59176)
                     /locus_tag="IW735_000062"
     CDS             complement(58949..59176)
                     /locus_tag="IW735_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1043094.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(59279..61867)
                     /locus_tag="IW735_000063"
     CDS             complement(59279..61867)
                     /locus_tag="IW735_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1043095.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(61887..62546)
                     /locus_tag="IW735_000064"
     CDS             complement(61887..62546)
                     /locus_tag="IW735_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1043096.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(62595..63098)
                     /gene="trmL"
                     /locus_tag="IW735_000065"
     CDS             complement(62595..63098)
                     /gene="trmL"
                     /locus_tag="IW735_000065"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1043097.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(63319..64185)
                     /locus_tag="IW735_000066"
     CDS             complement(63319..64185)
                     /locus_tag="IW735_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1043098.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            64294..64935
                     /locus_tag="IW735_000067"
     CDS             64294..64935
                     /locus_tag="IW735_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1043099.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(64997..66358)
                     /gene="mgtE"
                     /locus_tag="IW735_000068"
     CDS             complement(64997..66358)
                     /gene="mgtE"
                     /locus_tag="IW735_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1043100.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(66376..67275)
                     /locus_tag="IW735_000069"
     CDS             complement(66376..67275)
                     /locus_tag="IW735_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1043101.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(67277..68074)
                     /locus_tag="IW735_000070"
     CDS             complement(67277..68074)
                     /locus_tag="IW735_000070"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1043102.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(68052..68756)
                     /locus_tag="IW735_000071"
     CDS             complement(68052..68756)
                     /locus_tag="IW735_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1043103.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            68887..69462
                     /locus_tag="IW735_000072"
     CDS             68887..69462
                     /locus_tag="IW735_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1043104.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            69580..70257
                     /locus_tag="IW735_000073"
     CDS             69580..70257
                     /locus_tag="IW735_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1043105.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(70336..72144)
                     /gene="pepF"
                     /locus_tag="IW735_000074"
     CDS             complement(70336..72144)
                     /gene="pepF"
                     /locus_tag="IW735_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1043106.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(72320..73318)
                     /locus_tag="IW735_000075"
     CDS             complement(72320..73318)
                     /locus_tag="IW735_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1043107.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(73544..74197)
                     /locus_tag="IW735_000076"
     CDS             complement(73544..74197)
                     /locus_tag="IW735_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1043108.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        22357 a        15352 c        12687 g        24107 t
ORIGIN      
        1 agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aatttccgtg
       61 aatttcttct tatttcttgg agttaaacgc ttctgttcca gcctcttttt atctgtcttt
      121 cactaatgat cagttgtaga taagcaagtt gctttttctt acatcatacc gcccatcatt
      181 gatggatcca ttgcaggagc tgctggcgct gctggttctg gtttatctgc aacgactgct
      241 tctgtcgtta ataacaaggc agaaacggaa gcggcatttt gcaaggcaga acgtgttact
      301 ttagttgggt ctacgatacc tgcttcaacc atgtttaccc attctccagt agcagcattg
      361 aaaccagtgc ctagttccac gttcttcaat ttatcaacaa tcactgatcc ttcataacca
      421 gcgttttcag cgatttgacg aattggttct tctaaagcac gaacaacgat cttgatacct
      481 gttgccacgt ctccttctgc ttctacagca gaaactttgc tgataacgtt cactagagct
      541 gtaccaccac ctgagaccat tccttcttct actgcggcac gtgtagcatt caatgcatct
      601 tcaatgcgta attttaattc tttcaattct gtttcagtag ctgcaccaac tttaacaaca
      661 gctacaccac ctgctaattt agctaagcgt tcttgtaatt tttcgcggtc aaaatcagaa
      721 gttgtttcag caatttggtt tttgatcaat tgcacacgag cttcgattgc ttctttttct
      781 ccagaacctt ctacgatcgt tgtattgtct ttatctacaa caactttaga agcatttcct
      841 aagttttcga ttgttgcatc tttcaattct aaacctagat cgtcagtaat tactgtacca
      901 cctgtcaaaa tagcgatatc ttcaagcatt gctttacgac gatcgccgaa accaggagct
      961 tttacagcta ctacgttgaa tgttccacgg attttgttca atacaagagt tggcaatgct
     1021 tcaccatcta catcgtcagc aataatcaac aatggacgtg attgttgcaa gatttgttct
     1081 aacaatggca gaatatcttg gatgttagag atttttttgt ctgtgatcaa gatatatggg
     1141 ttttctagaa cagcttccat tttgtcgtta tctgttacca tatattgtga taagtaacca
     1201 cgatcaaact gcataccttc tacaacatct agttctgttt cgatcccttt tgattcttcg
     1261 attgtgatga caccgtcgtt tccaactttt tccattgcat cagcaatcaa atgtccaact
     1321 ttatcagaac ccgaagaaac agctgctact tgtgcaatgg cttcttttga atcaacaaca
     1381 gttgagatat tatgcaattc ttcgactgct gcttttgttg ccaattcgat cccgcgacga
     1441 atgcctagag gattagcacc tgcagttacg ttttttaaac cttcgcgaac gattgcttgt
     1501 gtcagaacag ttgctgtcgt tgtaccgtct cctgcaatat catttgtttt tgatgctact
     1561 tctgatacaa gttttgcacc catgttttca aaatgatctt ctaattcgat ttcttttgcg
     1621 atcgtcacac catcatttgt gatcaatgga gagccgtaag atttttctag tacgacattt
     1681 cggcctttag gtcctaatgt tactttcact gtatctgcta atttatctac cccgcgtaac
     1741 atagctgcgc gtgcgtcttc tgcaaatttc aattcttttg ccatgattga ttcacctcat
     1801 tgattatttt tgattatttt tttatcttcc aaatttactc aacaatcgcc atgatatctt
     1861 ttcctgcgac gatcagatat tcttttcctt cgtattttac ttctgtaccg gcgtattttt
     1921 cgaacataac ttggtcgcct gcttttacag cggcaggaac tttttcaccg ttttctaaca
     1981 aacggccttc accgactgca acaactgtcc cagtttgggg tttctcttta gcagcagaag
     2041 ctaaaacgat tcctccgaca gttttttctt cttcttttgc gacttcgata atgacgcgat
     2101 cacctaatgg ttttaacacg ataaatccct ccgataactc tttttttaat ctgttagcac
     2161 tcttaacctt cgagtgctaa cctacatttt taataatact cattctccct cttttttgca
     2221 agcttttaaa tcaaagaaaa gaaattttat tctgacagct gcttattgaa attatgatat
     2281 aattgaaatc aatcgatata agcttgcctt gcatgcttgt tatcccttaa aaggaggaat
     2341 tacatgtctt taaaaaaata tagcttcctc agcatttttt gctatggtct aatttttatc
     2401 tcacccttga tcctagcttc gataggactt gtaaaatcta cgtctgaatt gatcacagca
     2461 actgcagtca tgtatatact aggcgccgtt gtcctagcta ttttttattt taagcaacgt
     2521 gaacccttag cgatcgaatc agcggtaaaa aaagcttctg ctccaaagat tgttatttat
     2581 ggattggttg gaatctttgt cgcattgatt ctgcaaagca tcgctgtcat gttagaatct
     2641 gtattgtttg gcaaagcaac cccttccgag aatacacaaa atatcatcca gatgatcatg
     2701 gaagcacccg cttttatttt ggcgacaact gttgctggac ccatcatgga agagttcgta
     2761 ttccgccgca gtatactggg gatcatcagt cgatattcaa atttttgggt cggagcagtg
     2821 atcagctctt tgctgtttgc gtttgctcat aatgatggtc atctgctaat ctactttttc
     2881 ctaggtttct tcttcagcct agagtacaaa gcaactggtc ggatctggac atcgatgatc
     2941 acacacgtag gaatgaacac attagttgtt cttgttcaat tagctgtcca aaaagggcta
     3001 gtataaacat gactataaat actcctgtca tcctagattg gtgattttcc caatctaaag
     3061 cgacaggagt atttttgttt tttagtcgtt catcttttct gctgctccat cattttttgt
     3121 ctgtctttcc tttttgcagt ttgcagaatc tatttttttc aaaaatggga aataaatgaa
     3181 aaaactaatt gacaacacga taccttgtaa aatcgctgca ggcactccgc ctactaagaa
     3241 acccgagagg atcggcggtg ttgtccaagg gacttggact gctgtaaata aaggaacgaa
     3301 tccaaaatat aaagcggaat aggtaatcaa tcctgaaacg accggtgcga aaataaaagg
     3361 aacagccatc atcgggttca tcacgatcgg tgtggaaaaa ataattgatt cattgatgtt
     3421 gaaaaaggct ggtaaaagag caagatttcc taattctctg tatttcttcg atttcgcaaa
     3481 aaccgtcata aaaaatacag cccctaacgt caatccagca cccgtaaccg tcatgaactg
     3541 atcgagaaat tgctgagtaa cgatatgtcc gccatttgcc accgtcaact cttgtcctga
     3601 ggctaagatt tctgcatttg ctaatgaatt cgcttgcaag acgggactaa ccaacccatt
     3661 gaccatcgca gatccatgaa gaccaaataa ccataaaaac ggaacaaaaa aacctaataa
     3721 aagcacacca aacaacgtgt ctatgaacga ttgaatggga agctggacat atgtccatac
     3781 tccttgaaca acagtcattc tgaacaaatg ttgaaaaacc gctgaaccga taagcgtaaa
     3841 agtgactata acaatgattg gaatcaatga agtaaaagac tgccaaacat tttcaggtat
     3901 cttctctctt tgcttccacc ctttcgcaag tcttactaaa cgtgcatagc cccatccgat
     3961 gatgagccca gttgtaactg ctcctagtat cccttcagca cctgtccaga cagtatctat
     4021 ctgagtattt agtggctgaa taagcagtag ataagcagac aaggctgtta tgccgactgg
     4081 taagcgatcg atgtcttctt cttttgcata agcgtatgca atggcaaaaa cgatccaaaa
     4141 ggctatcaca tcaaatgtag cctggtatac ttgtgtaaaa tacatagaaa atcctgtttt
     4201 gtctatccac tgagcgatgt aatccacagg aaaatgagca aataagaaaa acagcgagcc
     4261 aacaataatt attgggatcg tgtaagtcat tccattacgc aaagcagtta agggtctaag
     4321 attcacaaca gatgttatca ttggtaaaaa ggcacgatct aaccactctt tcatttctta
     4381 tctccttcaa cttctatttt tatcaatcag ttttcttaat ttaactatac ccaatgatca
     4441 cgaaccatga aaggaatgcg cactgagctt ttcaaaaatc ggattctttg acgaatattc
     4501 agcaactgct tacaatggac aggaataaaa aaggaggttt tttatgaaaa ttgcactcat
     4561 tggagctact ggccatgcag gttcatttat tttagacgaa gcattgtcca gagatttaga
     4621 cgtgacggca atcgtgcgtc atccagaaaa attgccatca gatgttccgt ttatacaaaa
     4681 ggatttattt gagttgacaa ctactgatct tgcatctttt gatgttgtcg ttgatgcttt
     4741 caacgctccc aaaggaaaag aagaaatgca tcaaacttct ttgtcccatt tgattgagat
     4801 gcttgaagga accgatacac gcttaattgt cgttgggggt gcctcttcgt tattcttaga
     4861 tacagaaaag acgactcgta tgattgacca agtacctaaa aatgcaccct tctatccaac
     4921 agctttcaat atgtacgaag gattgttaaa attgaaacaa tctaaaaatc tacattggac
     4981 ttatatcagt ccagcttctt tatttgatcc acatggcaaa cgtacaggta cttatttgtt
     5041 aagtgatgat tatttacaaa gaaacgctgc tagcgacagt acgatcagca tggcagatta
     5101 cgcgattgca ttagtagatg agatactgga tgagaaacat gaaaagcagc acatcagtgt
     5161 agtcagtcga taatacggtt atttttttaa tatgctaaaa aaataaaaat agaagataaa
     5221 accaaccctg attgatttta tcttctgctt tttatatttt tattgattga actgtttaaa
     5281 tcgaaaatag ctcagttgct tcatctggat ctgtatcata gatttcaaaa tcatgtccaa
     5341 cgccaaaatc tttttctttc aaaacatttt ccattgttaa atgagcaagt tctttcatat
     5401 tgttttcctt gctaagatgg ccaagataga ttcgtttggt ccgattaccg atcacatcag
     5461 tcatgacaag tgcaccgtct tcgttggaaa gatgtcctct atctcctaaa atacgctgtt
     5521 ttaaattcca cggataaggt cccatgcgta acatctcaag atcatgatta ctttccatca
     5581 aataaccatc ggcattttca atgacacctc gcatataatc actgcaatat ccggtgtccg
     5641 tcaacatgac gaatgaccga ttttctttat ggaaacgata aaattgcgga gccgctgcgt
     5701 catgggagac gccgaaactc tcaatatcga tatcgccgaa tgttctgact ttccccatct
     5761 caaatagatg tttttgttcc actggtacat ttccaatcag tggttccatc gcttcccaag
     5821 tctgctgatt ggcaaaaaca tccaaatgat acttacgagc caagacaccc acgccatgta
     5881 tatgatctcg atgttcatgc gtcacaagta ttccatctaa ttggtccggt gtccgatcaa
     5941 tttgagccat caaggaagtg atttttttac cgctaagtcc tgcatcgacc aaaagccgtt
     6001 ttttttcact ttcgatataa agtgaattac ccgaacttcc acttgctagg atactgattt
     6061 taaaagctgg tttttcttca gccatttgac tcctacttcc cttcttactt tgtaaacttt
     6121 tttcattata ctcgcttttt tattgatctt ccacttttgg tacagtattg tttgtcacga
     6181 tcgtattgct gaaagcgttg atgctctcaa tttggaccac tttatcgctt gtttcgatcg
     6241 ctacatgcca aacaggaaca tacacatttt tttcccggac tttcagaata caggaataag
     6301 ctagctgacg ccataaaatt tttgatcctc ttggtatttt gttattgatg tacaatgtat
     6361 tgattgcttc ctcttccgta tacagttccg tcttttcgcg cagctgctcg atatcagata
     6421 aatgcgtctg catgtatttc gtgatgctat aatcattatc gtttttttct aaagaaagaa
     6481 cgatttttgc tgtatcatca ttgaatggga tgccttcata ctcttggctt gcttggattt
     6541 ctagtggttc ttcttctaaa tgagaaaagt ttttcgtgta cacataatca ttaccgaata
     6601 aaatattgtc tttgttagat aaaaaatctt ccaaggaact actaatttta tctggatcga
     6661 tcacgttctt ttcagcatct gaactggata aagatttcgt caaaacatca ccgtctaaaa
     6721 acacaccact tgccagcaag ccgcttttac cagccttgcg ataattcgct aatgaagtgc
     6781 taagattggt ctgctccgcg ctaagataat agccttgctt gttctcagac gaaatcttcc
     6841 ccgtgtaagt gatatcgtcg ctagccagac gttgttggat tgattcagtc tgattagagc
     6901 gaatgacatt gttttcttgg tataccgcgt catgataact gctgaataaa aacacattca
     6961 accctaaaaa agcgacgaaa aatatccatt cgattttttt gaaatccaat taattcgcct
     7021 ccaatccagg taatttttcc agaaggtcgt tagctgaata ccagttatct ttatatttca
     7081 cataccattc aggtaagaga tcaacgacac gattcgtttc tttgacattt tgccatgtat
     7141 agccaacgat cactgaacca atcttgttgc tatctgctcc cttagctttt aactgctgga
     7201 tgatttcatt cgtacttgca agttctactt cttcgtctga aggtatcggc acttgaatcg
     7261 tattcaaact ggtttggata ttcacttgca cagaaccatt cgtttcaccg ccaacttcca
     7321 caccaatctg tcctctgctg tctgtcccaa aaaccgggaa gccttccacg aatatccgat
     7381 aatcgatcgt attgtcactg cggtcaaaat atctgaggtt ccctaaagaa ctgcctaatt
     7441 tgctaatata ggggaaactt tcagaaaata cactagcatt ttcttttttc tctggcagct
     7501 ctccacgaaa atcaactact tgcgtttccg aatggataga cattgtttca ttcccaccgt
     7561 aaaaaatcaa ttcattgctt tcatcattat ttttcacgct atctggtttc tggaaaaaag
     7621 catttctaaa aagtgtatag ggttgctgtg acaggatata actatacttc ttcagtttga
     7681 tcgaatgttt cgtagcatat tgcgtgtcga tcgtcgatgg ttctgttgtc atttctgtcc
     7741 attttgtgtc ttttgctgtt aattctttat tgatatctgt ccaattcaac gaaatatttg
     7801 cttcatatac gagatggttc gaataattca aaaatcggat ttttttattt ccttgatcga
     7861 attgtattct agtaaaatac atattttcgc tttcttttgc caagttcgac acatctaaat
     7921 ctaaatcaaa tgcttccttg tattctctta gcaaaaaagg cgcattatag gccaactcaa
     7981 aaccagagtt cagatttttc acctgttcaa actgtgattt gtctcctgaa actacttcag
     8041 aaagctttcc aaatcttgct ttatcgatga tcaattgcaa tcggtacacg agattttcac
     8101 tgttggtttc ttggatcatt cccgaatggt gccatgtaac atgaagcgga agaaagactt
     8161 cggaagtttt tcgattgctt tgagactcga tcatttgagt gtttgtctca tctacgttga
     8221 ttgaactttt tcctgctggg ctcagccaga ttgcataaga gaagtatatg cttaacgcaa
     8281 ccatcaaaat gagtcctata cgaacgactt tttcagagat tctcattccc accattcctc
     8341 ctcataaggt tcatatggca gcgagatata aaaagtcgac ccttttcctt ccttgctctc
     8401 tgcccaaata gcgcctcgat gcgccttaat cacttctcta gaaatagcta atccaagtcc
     8461 tgtcccacct tgtgctcttg cacgtgcttt atctactcga tagaaccgct cgaacactcg
     8521 gttcagatct tttttaggaa tccccaatcc ttgatcggtg atacttaaaa tcacgttatt
     8581 atgcgtttct aataagcgac aagtaatcgt tcccccatct ggtgaatact tgatcgcgtt
     8641 attcatgata ttatcgacta cttggatgat tttatctgta tctacttcga cccaaagatc
     8701 tctttgcgta aattcacgac ggatcttata atttttttcc tgatttccca ccatcatatc
     8761 aaaccggtca agaacaaaat tcactaactc gttgaaattg acatattcta attgaagctg
     8821 ggtattacct gtatccatgc gagatagatt taagaggtca ttgatcattc gaatcattcg
     8881 gtctgtttca tctagcgtta ccttcaaaaa atttggtgct acttcctcgt ctttccacgc
     8941 accttcgctc aatgcttcga tataactacg catacttgtc aacggtgtac gaagttcatg
     9001 ggaaacatta gagacgaatt cacgacgttc ccgttctgtt ttttcttgtt ctgtcacatc
     9061 atgcaagaca gctactaatc cgctgataaa acctgattcg cgacggatca tagaaaagtc
     9121 tactcgcaag atcatctgat cactttccaa tgtactggtt gaacggtcaa gtaccatttc
     9181 gtttggatct tctaataatt tccgcaacgt atattcttct tcgatatcta aaagtgtcaa
     9241 aatcgattgt ccgattgctt cctcgttttt gatatctagc aaggaaacag ccatatcatt
     9301 gatcgtaatg actttacccc tgcgatctgt cgcaatgacg ccgtctgtca tatgggatag
     9361 cacgctatcc aaacgattgc gttcagcttc cattgtatct tgagcttctt caatacgttc
     9421 agctaactga ttgaaggtat cagccagctg tccaagttca tcttgtccat gcactttgac
     9481 tttccgactg tagtctcctc tagcgattcg aatggcttgt tctctcattt caccgatcgg
     9541 ctgtgtaatc gaacgagcaa ctaaaacaga tacgatcatt gaaataaatg ccgcaattag
     9601 tgaggcagta aagaaaatga ctgctgtgtt attgatttcc gaatattttt gttcgatatt
     9661 acttttgacg taaagagccc cgataacggc atcgccggtc ggggacagga tcggttgaac
     9721 attgatattc acccgtttat cgtcgttatc taaagcaaca taacgtttag tcgtgaaatc
     9781 attgatatca acataatcat ttttctttcc cacagcgctt tgttcattga catcggttgt
     9841 tcctcggatg atcccttttt cgtcaacgac ccgtatttcg atgatatcgc tagtcgcact
     9901 gttttcaata aatcttttga tattcgcatt tacctcttca tcatctgcat tatcttttcg
     9961 attcaattcc gggctgattg ttgttgctaa tgattccacc gtttgattca tatctttaat
    10021 aaatgtattg atcgtggaac gttctaaacc acgtataaag taggcaccaa taatttcgat
    10081 cgagatcaat aaaatcagaa taaaggtaat agcgatctta aaattgactg atcgaaaaaa
    10141 gcggactttt cttttcatcc aattactcct gttctgggtt acgcaaatag taaccgacac
    10201 cacgacgtgt cactaaataa tttggatggc ttggattatc ttcgatcttt tcacgcaaac
    10261 gacgtacagt tacatccact gttcgtacat ctccaaaata atcataaccc caaaccgttt
    10321 gtaataaatg ttctcttgtc atcacttgtc caaggtgctt cgccaagtaa aagagtagtt
    10381 cgaattcacg atgtgttaac tcgattgttt ctcctcgttt cgtcaccatg taagcttctg
    10441 gatggatcgt caaatcacca atcgtcaatt ccgcaggtgc tgcctcttct ggttccttcg
    10501 cagccgtggc accgcgacga agatttgctt tgacacgtgc aactaattct cggttggaaa
    10561 atggcttggt cacgtaatca tcggcgccta actctaagcc taggacttta tcaatttcag
    10621 aatctttggc tgttaccata atgatcggca tatcataagt tttgcgtacc tcacgagcaa
    10681 cttctaagcc gtccatttta ggtagcatca aatcaagtaa aatcaaatca ggttctacct
    10741 cagcaacttt ttccaaggct tcttccccat catatgcagt ataaacgtca tagccttctt
    10801 tggctaaatt gaatttgacg atatccgaaa tcggcttctc atcatcgaca actaaaactt
    10861 ttttcataaa gagccacctc ttatatatta ttgtcatacc ttctcattat accgtatttc
    10921 tgcttttgtt tcatctttgc ctataaaaca aaagagaaaa ttaaaatgaa agtgcaaaat
    10981 actttattat tttttgttct aacttcaata aaaaaggaaa acactgattt tcacctcagt
    11041 gtttcctttt tttgatttat ccttctaaaa cttgtacacc gacacccaat aatcccggac
    11101 cagtgtggac tactaatgcc ggagaaatct ggccaaagta gatttggttt gcttgtggga
    11161 actctctttt aagtctcgct tccatttcaa ttgcttcttc tttcgcatcc ccatgggcaa
    11221 cagctaagtt gaagaccttt gcattaccga ttttttcttt gacgtaagca accgttttat
    11281 ccaaactttt tttacgtccc ctagcttttc ctactgtgta ataaatccct tcttcattac
    11341 atgagatgat tgggttaagc tttaaagcgg ttcctacaat agaagcaact aaaccgatcc
    11401 gtccaccttt ttgaagatat tccaatgtcg caacattaaa gaaaactttt gttctttcga
    11461 ctaactcatt cagattactg atcagttgat cccattctac tccttcttcc agccattttg
    11521 ctgcttggat cgcagaaaaa cctgcaccaa taccaatatt tttcgtatct agtacatatg
    11581 cttccaattc ttcttgctgt tgtgcaatta aacgaaccac attatatgtc ccgctcaatc
    11641 cactggaaat tgttaccgca agtacctttt cataaccgtc ttttttgatt tgctcaaaga
    11701 tttctgtgat cgactcacca tccggcaaag atgtaccagg gatttcctct ggcaaacgtt
    11761 gatagatctc ttcaggggta atgtcaattt tatctgtata tacctgatct ttataaataa
    11821 ttttcaatgg gatcatatac atctcgtatt tttttatcaa ttcttctgga acatccgtac
    11881 ctgaatctac taaaattgcg attttattgt ttttcatttg ttttctcctc ttttaaatat
    11941 tcggtttcta gaacaatcgt tttttccgca agaagtttca ttgcaaacga taaagttgcc
    12001 gctcttacag caatatattc tacaggggtt gcctggtcga atttttgcgg atgagattta
    12061 ccttgtgcta attggctgac catccacaca gctgcttctt gttcatcgca aaatagatta
    12121 taggcttcac gaattcctac tgtttttcct tggaacaata tcccttcttt gatttcagga
    12181 atagtcaata cttgtttcaa cgtagtgatt gcaagtaaaa atgctacgtg ttctttgtta
    12241 tatctttttt taactggtgc tggtatcaaa cctagtttaa cgtaattgtt taccatagaa
    12301 gaggtaagca atggatgctt ttctccttgt atcacaggtg aaagataacg atcaactaac
    12361 gttctgactt ggtccatata aagttctaag tcagggagtt cttcccagtg aggcagacgg
    12421 aatgtttcta gttccttccc ccactccatt aattgctgac gcattttttc catcataacc
    12481 gctccttttt tcatttcata aacatgatat cagacttatg ataaacattc aatctagttg
    12541 ctttaactag gtgatttgat tagcgggatt ccatgaaaac aaaaaacaag aaggctaagc
    12601 cttcttgtct tttatcgtta agaacaggag acccgccggg gagacggcgg gaaaggagta
    12661 aaaaatgaaa aaagtgttag ggtttgttct cttgcaagaa ctttgtttgt tggtatgctt
    12721 atataatagc gagcaaatgt gaataatcta tgaatgattt cctataagga agtaaagatt
    12781 tttctgattt tctttaaatg tagtcttgca ttttaaaatt cttagtgcta ctattattaa
    12841 gtactttttg tacgggccgt tagctcagtt ggtagagcag ctgactctta atcagcgggt
    12901 cgcgggttcg agcccctcac ggcccattgg gtgccaaacc cacggaacag gtaaatgttg
    12961 gtgcgaaagc atttcgaaaa catttacgct gttctttttt tatttttcaa aataactagg
    13021 gctttttatt tttaactcaa agtccactgt agccctttgg actttgagtt aaaaaagaag
    13081 cataattatg aacagtaaag tttctaatat aacaataatt cgagaagctt tgcaaaatca
    13141 tgctgccaat gaactctcta aacatatagg attaaatcta agtactataa aaggttaaaa
    13201 agtgaagaat atttaataga aaaactaaat ttacttgctg ctttcccctt ttccattaat
    13261 acaacaatca gagagtgtct ttttttcgaa aaaattagaa taaataaaag acacgtagtt
    13321 tttttatcta catgtcttcc atcgactaca agttatcatt tctcaaaacg tatttagctt
    13381 ttaattgatt aatcgtttca cgttgtctac acattgccta attaagattt tcttattaga
    13441 agcatcaatc ccgcaataat acctaaaact ccaggaatta cccacaataa tgttaaaaga
    13501 aaaaatggaa taatagataa aatgagtaaa ctaataccat agaccactgt tttttgattt
    13561 tttcttacta aaaaacctat tatgaaaatc attatcgaaa aaactagggt aattcctcca
    13621 aataaaacaa cattcccgct tagattgact aacatatcta tatccttact ggttagttct
    13681 gtagtattta aagtagttga atcaatagaa gatagaaaca taccacccat caatgctaaa
    13741 agactacata aaatccctgt tattccacta attaaaatca aaataaactc tgtttttctc
    13801 ttattcattt aattctctcc agattaagtg ttttagaatt agaaattttt tgaccatagt
    13861 taatagcttc taatgaacga ataacaacca ctgacgtgtt agaaaaataa tccccaatat
    13921 gttgtttctt gggcgtgaaa gcagccacca aatacccaat ttgaaataca aaatttttta
    13981 atataaacct acatatacac tcacgaataa agatagtttg ccaacttagc tctttttcct
    14041 caaagctcac tactcttatc ccaaaaatca ttttcccaat tgtttgtcct gaattcagct
    14101 tagtcaaaag tacaaaatag gttaaataaa tagctaatga cgctatacca taaaaagtaa
    14161 taatggattt gtcagattcc agtcccatga ttttgtaaaa gagacctata gtagcatggg
    14221 tgatgatgct aatacaaatt aaatcaataa gaaaagcaaa aaatcgtaac caaaatcccg
    14281 caaatacata attaggcaaa gtttcaatgc cactcttctt attcatttta tagccctcca
    14341 tagtaataca ttggttttgg agtatcagaa aatccaagtt tttcaaaaac tgaaattaca
    14401 cgatctgatg tagttgcctt tagcccttgc cattcagcaa tcttattacc taaccaagaa
    14461 gaagcaaagc cagttgtgtc atttttatat tctataaccg tcgcatcagt taatttcttc
    14521 tcttttttta gggaatctaa tgcatcttca gagtaaccaa tttgatccac caacccattt
    14581 tctactgctt ggttaccatc ataaattctt ccatccgcaa gttctttcac tgcattagta
    14641 tccatatttc ttccttttgc tacaacattg acaaaacgat cataagcact catcacaaat
    14701 tcctgtataa ctttattctc ttcctctgaa ggtttgtgtt gaggtgacat catatctttt
    14761 aatgcaccac ttttatacgt cgcatcagtt acgccaagtt tttctaataa gccagaataa
    14821 tttaaaccag aaataatgac accaattgat ccagttgttg tttcttccgt agcaaatatt
    14881 ttatctgaac cagcagaaat gtagtaccct ccacttgccg ctgtattttt aaatgcagta
    14941 tagataggaa tatctagctt tttaattttt gccatttctt tagcaatctc agcactttca
    15001 tatattcctc ccccaggtga atttacttct aataacactc ccttaaccgc tttgtcttct
    15061 tgaattttct ttagctgtgt taaaaaattc tgatggttat attgttccct actaaaaaga
    15121 ttgctttcac ctgtatctgc aataactcca ttgacggata gtttgacaat ctttttattt
    15181 gatgcgcctt cttctaacgt ttcttctgtg atttcgttag atccgtaaaa aatcttattt
    15241 attcctgtta gggttgtatt tgatgcttcc tctttatctt ttcctggaat tagcgcgacg
    15301 ataatggata gtaacaataa tccgcacgct actcccacag caatccatct tcttttattc
    15361 atataaactc ctcctatatt cttagtaaaa ataattattt tttatttaga aacacatcga
    15421 caatttgttg ccaatatgtt ttttctccta caattaaaga caactctcca gttaatccat
    15481 aacgaaacgg aaacgattga gttgttttta acgttccttt cactgcataa aaaacacctt
    15541 tctcagtggc agtagaattt tctgaaattt catctaaagt tccgtcgatc gtaatcggag
    15601 ccccaccttt ttgatccaat ttaaaatgaa ccttcatccc ttctttaata taggtacttt
    15661 cattcgcagg taaaagtgct gtaaatttca tttttgattt ttcgtggatc ggataaattt
    15721 ctgcaagcac tgttcctttg gctagttctg cttgtccctt tgtttcttca tttaaatgaa
    15781 ccgttccaga tacaggagct tttagtattc cttttgaaag ttgatcgtcc aaacttttga
    15841 taactgactg gttcttctct tgtttttcat ttagctctgt aactgtttgt tttacagttg
    15901 ttattgcttg ttctagctct tgctttcctt tttccgtttg actaattctg tcattctcag
    15961 aagtagttgg tttaactaat ttagcttttt ctgactgata ctgttctact tcttttttta
    16021 gttgctcaat ttgttcattt atgcttgctg aaattgtcag tttcacttga tttttttgat
    16081 catcagaaac attatttagt tgttcttgcc aattctcata gtttgccatc atttcttttg
    16141 aaaaatcttt taatgattga ttattactcc aagctacctg tacttgttgc cattcttgta
    16201 atttactatc tgaactctca atttgttttt ctagttgtct ttttgtcttt tcgtatactt
    16261 ctaattgctt ttgatcattt aattcacttt gcttcaaagc gtagcggagt gattcttttt
    16321 ctgacaacat gctctttaat tgattactgt agccaaaact gtcatccgtt gaaaacaaat
    16381 tttttccttt acttatactt tcaataaata gctgagccat ctttatttgt tgagtaatac
    16441 gttggttttc ttgttctatc tgagcctttt cattctctat taaagaacaa tccaatctaa
    16501 ccaacacatc gtccttatga acaaattgat tttcttttag gaagttttcc attattttag
    16561 cttctaatgg cacttgtaac ttctctattt tttcggggtt caattgagcc gttgtccgaa
    16621 taacaacctc ttttttagca aaggttaaaa acaatccaat aatacataaa aaaagaatta
    16681 cagggtataa cacccaacgg tagaagctat gatgttgttt agtatagata ctagaatgtt
    16741 cgaaccaatt attattctgc ataaattgtt tccctctcct agatttccca taatttttga
    16801 tagataccat cggcaaaacg aagttcatcg tgcgttcctt tttctaccaa atgcccttca
    16861 tggagaacta aaatttgatc acatgctttt gctattggta aatgatgcgc aataaaaata
    16921 atcgtttttt cttttaaatt gaggagataa ttcataattg catgttctaa caaggtatct
    16981 aatccactag ttgcttcatc taaaattaat atgtccggat tttttaataa agcccgtgct
    17041 aaggctaatc gttgtcgttg ccctcctgaa agattatttc caccttcttc caacaatgtc
    17101 tcgtaacgta atggctgttg attaataaat tcatttagtt ggactatctc acaaacttct
    17161 attatttgtt cgaaacttgg tgggctatct aaaccgaata taagattctc aatgattgtt
    17221 ccactaaaga aaaatgactc ttgaggcaaa tatgtcactc gatcgcgtaa tatctgaaaa
    17281 ggaatgtcta agtgattaat gtttccataa tggatcgatc cttctgatgc ttcataaaaa
    17341 cgaacaaata actttgcaag agttgatttt ccagaaccac taactccaac tatagccgtt
    17401 ttactgtgcg aaggaattgt acatgaaatt tcgttcaacg ttggggcctt catgctatac
    17461 gagaatgaaa cgttttttac agtgatatct tgttgaaata attgttcaga aagcatatat
    17521 tcaaaagact ggttcgattt ttctgaatca atcgcgaaaa tttcatttaa acgattattt
    17581 gcaacttgag ccgtttgcat tttcacttgt aaattaataa tgttttgtaa aggatctgtc
    17641 aaaaatacta gtaaagcatt gtatgtgata agttgaccta gactaattct attatccata
    17701 acaaaataag aacctagcca taaaattaat gcactgctaa ttaattgaat caaatgttta
    17761 attccttgtt gaatattatc aagcgtaacc gtacgaaatg attttttcat taatttaaca
    17821 aattcatggt ctatacggtt atatactttc tcttcaccgc tatatgcttt aattgtttct
    17881 attcctttaa gactttctat gatactagaa tttacagctg caccggctgc catttcttcc
    17941 ctgttcgatt tttcatagct tttcacgaaa gcgaatattg caactacata aaaaggcaaa
    18001 gagcatagag tgattaaaaa caacgtacta ttctgaatag ctaacgtaac cccaacaagc
    18061 aacaccatac caatatctaa aaatattgat aacgtagcac ttgctaaagc atcaataatc
    18121 ttactggcat caagaaaacg agaaataatt tcccctgatt ttctcgtagc aaaaaaattc
    18181 attggtaaag ttaacacatg tttgaaatac tgaagcataa ttgccatact cattcgctgt
    18241 cctaaaataa taagaagata actacgacta tattcaaaca atacccgaaa taaatagaca
    18301 gcgatcaagc caatggaaat tatatttaaa gttgaacgtg cttgattagg gatgaaaaaa
    18361 tcaagaatac cttgaaaata atatgagctt cctatgccaa aaaacgtaat gaaaatcgat
    18421 gctaaaacga tgtggaatac taatcctttt tgtttccaaa ttataggcaa aaaagaactt
    18481 aatccagcta ctttttcttt aataggaata taagtttctc taggcatagg taataaaaga
    18541 atacctgtcc attctttttg aaacgcttca atcgtttttc tattttttcc attagcggga
    18601 tctgcaatcc atagtacgtt tcccttcgtt ttataaatta caacgtagtg catatagctg
    18661 ttatcaacca atatatgtgc aattaatggt aagataagat ctctttcttt ccagatctca
    18721 ctatctgctt gaatagccat acaatcaaaa gacaattttt caaatgtctt tttcaatcca
    18781 aatgctgaag taccctctaa atcagtccca gataggtccc gtaatttatg aatcgggata
    18841 tcagttttaa aatattttaa aatcatcgaa aggcaagcaa caccacaatc tttttcatct
    18901 tgttgctgca caaaaggaaa tgattttatc acttatctaa cacaactcct tctaaaaaat
    18961 ttttatagaa aaattctcaa tatagcttta atacagtgaa aatattgtct cataattcca
    19021 caaaaacttt gaaaacagtt cttgaacact aactttttca ttcctgaatc aaatttattt
    19081 tggaaatttt actattcaag aataaacttg ataagtttca ggaagatttt tactgttaat
    19141 caaatacgta tgttacagta gcaccgtaaa aaatattatt taaagaagcg tgtttaaaca
    19201 tgcaaaatgt aaaggaacta agtattgtgg aaatgcacaa actgttggtg gaggcaataa
    19261 tgcttgggga aaattggggc aagttgtagg tggacttacc acaggggcag taggtggtgc
    19321 aggtttagga acagcaattt gcggaccagc ttgcggtgtt gttggagggt tatatggagc
    19381 tgtcgctgga ggtgctgcag ctggctggga tgctcgaaaa aaataaaaaa ggagaataag
    19441 tagtatgcat attaaaaaca caaaaacaac atttatatta tcttcagaag agttgaaaaa
    19501 tattcaaggt gggagtgccg tgggagtttt aggcactact ttttctgggg caacagcagg
    19561 agtaaaactt tgttcagccg gaggacctta tgcaatagct gcttgtggtg taggtggagc
    19621 tttattaggt gctggattca gcatgtggac cggtgcgtaa acaattaaaa aatatcaaac
    19681 ctcaagattg gtttgtgtcg attacgaaca taggtatgtt ctttatcttt tcaaaagttt
    19741 ggaatggaca gaacttttta ctaaagcttc ttgtgctagt ttttataatt atattaaatt
    19801 tttatctttt attaaagtca gataaaaatt ctttggaaat gtaatggtag agatattttt
    19861 acaaaaggac ttgcagcgct gtagttggtg ctgcaggagg acctatttcg gccgttacag
    19921 gaggtattgg taatgtactt gtcggatgtg cattaaagcc tgttcctgca agttaaattt
    19981 ataaaaacag gaaagtaatt atctcaaatc ctatcaagta caatcatagg attgcacttg
    20041 acaggacaaa ttttttaact aaaaaatgaa aaacactttc taaaaattag tagaatttgc
    20101 caaatctttc gcatcttcca ccatacattt aattttaatt cccctttatg gtacttaata
    20161 aaaacaagaa aagaactatt catttggccc ttttttggtt ccagaccata gaaatattcc
    20221 tccagtaact aatattgctg ttatgcacgt aactaataac agctataaaa attggtacaa
    20281 ctttaatagg attcagaaaa atttgcacta gtgaaggaat ctattttatt agaaaaatgc
    20341 aatctcccta aagaaaaaac cactaaacag tgctttcttt caacttctcc ttaatttttt
    20401 gtgctttccc taaagtaaag tttctggcca actctgaatc aaaatatagt gaacgttttt
    20461 taaaatctat ttctttaacg ttaaagatat ttactaaaca cgctcgacta attcttgtta
    20521 acataggata tttcttttca agctcactaa ttcttccata aaattcgtac tgcccatctt
    20581 tagtacacaa tgataatcta tgtggtaatt ttgaagcttc tacaaaataa atgtcattta
    20641 tatcaaaagt aaatgtttga gaacctatgg aaaaaacaaa tgcttgattc gtaacctgtt
    20701 ttgttgcatc tatcctttct tgcgctaata acaataactc aacaatctcg tttcgatact
    20761 catctagtgt ttgatctttt gttacaaatc ctaacgtttc aactcttctt tttattgtta
    20821 cgggtaacat ctcatcatga gtagttgtaa aaatgatttt tgcttgaaca tcatattttc
    20881 tgattacttc tgctaattct ataccgttaa cttcatgatt taaatcaata tctaagaaat
    20941 atattccttg ctttggtcgg aattgtttga ggtatttttt aacttctaac ggactttgtg
    21001 ttttcaatac tatcttaaat cctgaataat tcatactttt aattaaaacc atgtgaaact
    21061 gcctcacggc agcttacaat tactttttca tcttcacccg ttaagtgatg aaaaaagaac
    21121 ccctttctgc tatggttaaa gtgactaaac caaccaaaag aaaggagttc ttttcatgac
    21181 tagcttacac aaaaaccaag taaaattcaa ttcaaatatc actatttctc atacaggtgg
    21241 tcaattatcg agtgattcgg gtctcgtcct agtgaaagag ctgatgaaca tctttggttt
    21301 ttctgaactg gctaagcaac acattcacat tgaagacaaa cgagcgtatt ttactcatga
    21361 caacttatcc atacttgagc agttcattat gcaattaatt gctggttact cagctgattc
    21421 tgcagctaat ctcctgagac aagatcctgt gtttaaagct gttttagata ggaaagaact
    21481 cgcttctcaa tcttcacttt cccgattttt agatcgacta tctgaggaga atatccatga
    21541 acttcaagct ttgaaccaag aacttatcga taaagcacgc ctcatccgta atgatacgga
    21601 attaatcatt gatttagatt cgacccattc agatacattt ggtcaccaag aacaaacgaa
    21661 ttataataca cactaccaaa cctatggtta ccatccattg gtagcttttg acggattgac
    21721 tggtgacttc ttaaaagctg aactacgttc aggaaatcaa tatacatcaa aaggcgtaaa
    21781 ggagtttctc acacctttat tagagcacta taatcactct ctaccaaaca ctgacatctt
    21841 ggttcgtgga gacagcgggt tcgctacacc tggtgtgtat gattcgtgtg aatcaaaaaa
    21901 gagtcattat gttattcgac tgaagaataa tcgtagacta ggtcaaatag ctgagaaatc
    21961 agtactttat ggcgataatc aaaagtggga agaacgagaa gttcagtact tctcgaccac
    22021 ttatcaagca caatcctggt cacaaagtcg tcgcgtgtgt atacgctcaa cacgtgaagc
    22081 gggcgaacta ctctttcgac atgaatttat cgtgacgaat ctatcagaaa atgtttctcc
    22141 tgataccatc ttttctatct atgccaaacg tggcacaatg gagaacttca ttaaagaagc
    22201 gaaagctggc ttttactttg acaaaacaga cagtcctcgc tttttggaga atcatgtccg
    22261 aatgatgctc agcttgatag cttacaactt agtcaacttc ttaagaacga ttggctttga
    22321 agaagtccaa aagggaatga ccattcattc tattcgattg aagtttctga aagttgctgg
    22381 gaaattagtc caaacgggta gacgagtcta tctcaaatta tctagttatc atgtgtatca
    22441 gaatgaattc tacagggtct ttgctcgcct gaggcgagcc agtcaatgga tctaatccaa
    22501 caatctaacg atttttttac tgggaagtta tccagggaga agtatgctca aaatgcctta
    22561 cacccaaaat ttattcctca atatttttga acagtgaatg tttgaagtaa ctttttagtc
    22621 aaaatcagtt gatagggata ttagtgatac atatttcaat aaaatgtgcc aaagaattaa
    22681 agctatgaat tattcaggtt aaatacttta tcgtgaaaaa gaatgaaatt atcaataatt
    22741 cgttctaact gatttaattg tataatttga tcttcacata atattattgg ataactcata
    22801 aatcctcctt ccctcaaaac aacaaaataa cttgagtagt aaattgatac ggatcgtttt
    22861 gatagttcac aaacatattg ggattctttt tattgatttc ctctatacta gataaaccta
    22921 acccttgatg tccctttttt gttgttattc cttttttcat tagagtatct atagagagta
    22981 gtatctctct acaactattc gatatcgaaa tttctatctg tttatcatct cggtaaatag
    23041 ctagagacag ttctctatct tgactctcta tagatgcttc aattgcatta tcgagaataa
    23101 ttccaagcac cctaatacag tcaaatattg gaacaggaac ttcaataata gtacttggac
    23161 attcgaattt acaacaaatc ttattgttat ttgcttggta tagcttcgct atcaacaaac
    23221 ttttcaaata ttcatttttt atatttttta aataacgata ttcaaactta ttggttagat
    23281 aagaattaga atagatctct aattctttta tttctttttc caatatcgaa tcatggtttt
    23341 gattagcgat ttcttttaaa ctcagcaata aatttttata gtcatgtcta aatctggcaa
    23401 atttttcttg atctttttcc agtgaatcag tatatttttt tagataaacc aattcttttc
    23461 ttttcaattg ctgttcatat tgttctcgtt gcctaatagt tattctaata aataaaaata
    23521 gaacaacaaa catttgaatg attaaaaata ttaagacacc aaacacaaaa tgatcaaata
    23581 cttgatattg atgagcaaca taagaaacaa ataaaccaat tgtaagtaaa taaatcatga
    23641 atatagcagt aagtttagaa ctggactgtt caacaatcct atttatagct aattttctat
    23701 ataaccaaat aaacatcagc aaaaacaaag aatcaagcat tatctggaat actacataac
    23761 catacccttt tatcccttca aatgaaaaca tatacgtcat aaccatggaa gtaataatag
    23821 atctgaaatc atcaataagc atacataata gtaatttatt taaaagcact atattagttt
    23881 tcttcttttc ctttaatata aaataacttc cgattatagg tatagctgcg gaaacatcag
    23941 taaataattc aacaaaaaaa gtgagtggta tcaataaaca tacaaaaaga tattttcttt
    24001 tgttaagatc agtattagtc atataaaata agagaaaaaa gaaaaagttt cccacgaaca
    24061 ttcttaatag ataagctttt gtatcgatag taatcaagag atcccctcct gattttagga
    24121 tatcataaaa aaaattttag ttacactttt taaataatga aaaaacacat tccactaaat
    24181 ttgatgccgg ttttccttga ggttttgttc cagcacctcc tttaattttt ttaagctctt
    24241 gatctgaaca ttgtttagca tttagtctat ttttttcttc cattacgatt ccccctacaa
    24301 actataaata cttttatgtc actgattcaa tatacctatt tttctaatca aaaaattttg
    24361 agacatattt aatattataa gtatcattaa aattgagatt tatctccata atctgctcga
    24421 tataaaccat ttattccagc agttcttcca atttcactca aatttcttat taattcctct
    24481 tgatattcag tgaatcttat tttattagta taagcagtaa aatatagata attcaccaat
    24541 ctatttatta aaggaccggg atctaattca tatttttgtt tttctaattt taaatatacc
    24601 ttttgtaaaa cctcaagaat atcagaatat ttaggatctt tacttataga tatatcatta
    24661 tataattcat gaacaatttg cttagcattt tttttcattt tagcacttcc ctggaattgc
    24721 tccacctaaa aaaccaccta tagacattcc tgcaatacaa gtagttgcct tggcccaatc
    24781 gaccgtacat ttatttttag tgcaatacac tccatttcca taatattttc cactatgagt
    24841 ggtaccccca tagataagtt gtgtctcttt aatagacaaa atttttaaat gtttcataat
    24901 ctaatcaccc ctaaattaat gtttttagac tattattcta acattcatta aatttcaaaa
    24961 aacaattcct tcttgaaacg tagcaaaata attcctgaat aagaatttaa tcacttttaa
    25021 gtatataaac tatgtatatt ctttaatatt ttttgagaat gcacttcgta cataataaac
    25081 tttaatcaca ttagtctttc atacctacga tcgatctctt tgactcgcac ccataaattc
    25141 ataatagttt gtcggcgtct ttggacgttt cgaagatgag ctacgcattc tcttttttta
    25201 tagacaaaaa agatagagac gaacatggag cttccaaatt tggatatttg atccaatttt
    25261 ggaagctcca tttatttcgt gtctgtcttt ttattttttc cagctttcga ctaattcttg
    25321 gttctctttg atccagtctt ttgctgcttg ctgtggatct tttccattgt tgatttccaa
    25381 cataactttt tccatatctt tttctgacca atggaaattg tctaatactt tgtaagcttc
    25441 tggctggtct tctttcaatc cttttcgaac catcgtatgg atctgctctt cgcttcccat
    25501 tccgttttca ggatcttcta agtatttcag atcatatttg gcaaacatcc aatgaggtgt
    25561 ccatcctgta actacaattg gttcgtgttt tttgatcgct tgacctagtg caacggtcat
    25621 tgctcctgaa gaagatgtct ctactttcca gtctttcaag ttgtcatatt tttggatcgt
    25681 gttttcagca gccgtaacta caccagctcc tggctcgatt cctatgattt tcttacctgc
    25741 ctgatcggtt aactcatcga tcgaatttac atccatgtag gctggcactg ctaaaccaac
    25801 ttttgctcct gtcaaattgg cacctaggtc ttcgacttga tctttatatt gtgcatattg
    25861 tgaaccatga gttttaggta gccaagctga aaccatggca tctgactcgc catttgaaac
    25921 agatttccac atgattgaat tatctaatgg cgtcatcgtt acatcatagc ccatttgttt
    25981 caatacttca ccgactacat ttgttgaagc aacttctgta tcccattcaa cataagataa
    26041 gttgatacgt tttgtttctt tggcagatga aaaactccct ataccaatcg cacctaagat
    26101 cactactact acagcaccaa tgatcagtcc ttgacgtttt ttacttttct gtgttgttgc
    26161 tcctgcagct tttttattgt ttggctggtt caaatgctgt gtaaaacggt caatgatgat
    26221 tgctaagata actaatgcaa ccccgttgac aaatccattc ccgacttgtg ctctttgcaa
    26281 tgctgataat acgccgcgtc ctaagccggg tgcaccgatc atagaggcaa taacgaccat
    26341 tgaaagtgac agcattgttg tttgattgat ccctgccatg atcgtacttt tagctaaagg
    26401 caattctagt ttgaatagtt tttgacgact tgtgctcccg aatgaatcag aagcttctac
    26461 taattcttta ggaacttgtc gaatccctaa attcgtaaaa cgaacggttg gtggcagagc
    26521 aaagatgact gaggcaaata cccctggaac catcccgata ccgaaaaatg caactgctgg
    26581 aatcaaataa acgaatcctg gcattgtctg cataaagtca aggatcggtt tgataatact
    26641 ttgggcttta ctgctttttg ccatcaagat ccctaatggt accccaatga tgatcgagat
    26701 cacactagat aaaagaacaa gtgtgacagt attcatcaaa tcattccata gaccttggtt
    26761 ataaataaac catagaccga ttaatgtgaa caacgtaagt cctggacgtt tcttagaaat
    26821 aaagaaagct gctaccgtca ataacaagat aaacaaagga gctggaatca ctaacagtaa
    26881 atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt aaaaatgaaa ataggccaga
    26941 aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag tttgccactg gtaattgata
    27001 gttcaataga ttattcatgt tcactcacct cgcttgtttc tgctaatgct tcaatcacac
    27061 tacctcggat gacgacacct tttaattttc catcctctac aactgcaagt ggagtcggtg
    27121 agtcataaat aatattgaaa atatcactta ccaccatgtc tttcccaatc accgtcacat
    27181 tttcatctag gaagtcgatc aatggacgtt tttgcttcct agcttctaac gcttgatctg
    27241 cagtgatgat ccctttcagg tgtctctttc tgtcaacagc catcagcatg ctgacttctt
    27301 cattacgcat ccttgtcaat gcaacgtttg gtccatcaga ttcgatattc gtcgtcaatg
    27361 caggtaccat gatgttttga gccgtaagca ctttggaacg atcgacttct tccacaaatt
    27421 cacgtacata atcatttgct ggattggtca atatttcttc acctgttccg atctgcatga
    27481 tttcgccgtc tttcatcaag gcgattctgt ccccaatccg taaagcttca ttcaaatcat
    27541 gggtaatgaa aataatcgtt ttttgaacat tggcttgcag gtccagtaat tcgtcttgca
    27601 tttctcgccg gatcagcgga tcaagagccg agaacgcttc gtccatcagc aagatctcag
    27661 gatcatttgc taaagctctg gctaggccaa cccgttgttg catcccacca gataactgac
    27721 taggatattg gtctttaaat gaaagcaaac tagaattttc taatgctttt tctgcttttt
    27781 cttgtcgttc ttctttaggt acaccacgga cttctaaccc gtattctgta ttttccaaga
    27841 tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat attgatttta tgacgacgga
    27901 cttctcgcaa ttcttctttg cttaacttcg atacatcttc tccatcaata tagatactgc
    27961 ccgaagttgg ctcaatcaga cgattcaata gacggatcaa tgtggatttc ccactcccgg
    28021 aaagccccat gatcacgaag atttctcctt cttttacgtc aaaattcacg tcatacacac
    28081 caaccgttgc accggttttc tttaaaatct ccgttttcgg ctggtgatca ttcatcatat
    28141 ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa atgttttact tctactttta
    28201 ccaaacaaat tctccttttt tatgtaaatt catttttctt tctgaaaaaa gtgaccacac
    28261 tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga aatttgcttg tccaaactga
    28321 aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc aagttaatat ttttttaata
    28381 tttcacacaa tcattctgca agctgatgct ccgggtaaaa aaagtttgtc atacgctgta
    28441 gtgtatattc atttccaata aaatgaacga tgtcccctgc cgtaaattta gcataggggc
    28501 caggtgaaac aagcagttct tcattatgag aaattgcgat aactgtggcg ccagtcgcct
    28561 gccagatatt caactcgcta attgaacggt ccagatgcat tgctttttct gataaagata
    28621 attcaaacgg aagtaaagga ttcatcttat gtattcgctt cgtttgattg accagctgat
    28681 ccaataattg agaaaaatga ttcagctcat cttgttgctt ctccacactt gccatgattt
    28741 ctgcttttgt atcttggatg gattgtacat cttgatattg caataggaaa tcttttgctt
    28801 tttccttcga agcaacaaat gccccacttc catgccgtac ttccatgatt ccaagatcta
    28861 ccagcacatt gatagccttg cgggtagtct caggagaagc attgtacgta ctggcaagtg
    28921 ttgagcgggc atggatcttc tcacccactt tatatcggtt ttcaacgata cgttcggcta
    28981 aatcaacagc aatctgctgg tagcgcgggc ggacgatttt tttctttcca gacattttct
    29041 ttcctctttc tttttttact ttccctcatc ttactatgct ctagcaaaat aagcaaaaaa
    29101 ggggagaata gcctcttgca acgactgatc atcgcttgat ctgttccttt tttactgggc
    29161 aagctgtttg acagtcatca caattcttac cggattttaa acgagtatag acaattgcgc
    29221 ctgcacttcc aaagattaac acacttaata agaatgtcgc catcctattc ctctccttct
    29281 aatgacaata tagtataaaa ctggttcttg gattttggct gacggaacaa aacataaacg
    29341 agtcctatca tcagtaaaat agcaagatac gtccctaacg ctacctgtcc accctccaat
    29401 aagtgtccca actgataaat cacaaaacta actaaataag ctaaaccgca ttgatagcct
    29461 atggcgatcc aagtccattt cttggcattc atttcccgat ggatagcccc aattgccgca
    29521 aaacaaggcg cacaaagtaa attgaaggct aacaatgaat aagctgctgc gggagtatat
    29581 gtgtgttgta gcacttgcca gatttctctg ccattttcgg atacttccgc atgaccaaac
    29641 aaaataccaa atgtgccaat aatattttct ttagcgacca atcctgtaat cgctgcgact
    29701 gttccttgcc agtttcccca accaagcggc tgaaaaacaa ctgccaggat tcgcccgaaa
    29761 aaggctaaga tactttggtc ttcagaaaca ggttgagcat aaaaattata agaagagctg
    29821 aaccagatca cgatactaga aacaaagata atcgttcctg cttttttaac aaaagacttg
    29881 ctgcggtcat aggtttgtct taggaccgct gaccattgag gaaaatgata tgcgggcaat
    29941 tccatgataa aaggagctgt atctccagca aacaaacgtg tttttttcaa gctgattcca
    30001 gagaaaataa ttgcagtcat accaagaaaa taggcagaag gagctaccca agattgatga
    30061 ggaaaaaagg cccctgctac taaggcaata atcggcaact ttgcagaaca aggcatgaag
    30121 gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt caatcgtgcg actggccatg
    30181 acgcctggca ctccacatcc tgtagcaatc agcataggaa tgaaggattt tcctgaaaga
    30241 ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc tcgccatata tccgcagtct
    30301 tccagaaagc tcaagcataa aaacaatata atcaattgcg gtagaaatcc taacactgct
    30361 cccacgcctg cgatgatgcc gttcaagatc agctcctgca tccatcccgc tacttgccat
    30421 tcagctaaca atctgccaac atgttctggt aaccatgtac caaacaatgt gtcattgatc
    30481 caatctgtcc ccattgttcc tactgtttga atagccagat aatagatcag ccacatcaca
    30541 aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa tctgatcgat tttgtcactc
    30601 atcgataatt ggaaagaatc atttgacaca acacagagtg tacatagttg ggtaataaat
    30661 tcataacgtt cattgaccag aatcgtctca ctatcgtcgt ccaatagttt ctctgttaac
    30721 tggatcagtt gctcgatctc tttttgctga gaaactgcta tctcgtaatc ctctcttacc
    30781 ttctgatctc gttcaaaata tttcattgca taccagcgtt tcttgccgct ttgttctcct
    30841 agtacatctt caatttcatg taaagctgct tctaaacgtg gatcatatgt acagggaaca
    30901 ggaatagaag gccgcttttt tgcagactcc tgaatctttt cgattagttt ttcgattcct
    30961 tgctttttca atacgctgat tggtaaaact ggtacaccaa gaccatagga aagcttttct
    31021 agattgattt tttttccttc ttttttgatg acatccatca tattcagacc aatgatcata
    31081 ggaatgtcta aatccaataa ctgcgtagtt aaataaagat ttcgttctaa attggtagca
    31141 tcaataatgt tgatgatcag ttcaggctgt tcctgaacca aataatctct tgcaatcact
    31201 tcttctggtg tataaggaga caaagaatag atgccgggta ggtcctgaat atgaatgttc
    31261 ggattttttt tcaacgatcc ttcttttttt tctactgtta cgcccggcca attccctaca
    31321 tattgattcg aaccagtcaa gagattgaac aagctagttt tcccgctgtt tggatttccg
    31381 actaacgcga tattcattct ttttcctccc tttgtatcaa gattagttcg gcctctgact
    31441 ttcgtaacgt cagttcatac cctcgcactg tcagttcgat tggatctccc agaggggcta
    31501 gttttcggat aactattttt acatttcgcg tcaatcccat atccattaac cgtcgcttca
    31561 ctgcgccttg tccgtggatt gctacgacga tcccagtctc tcctgctttt aattgatcaa
    31621 gagaagtcca gctttcagag gaagcagtct ttttattgac aaagatttga cctagtattt
    31681 cccgacttac tgccactcga tgacttttta ccagtaaaat ggcattgttg cctgataaat
    31741 tgacgacgac gattttactt ccggcaacga tcccgatatt ttgcaaatgg cgttttgttt
    31801 tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt ctgctttgcc tgcgctaagg
    31861 tctgcatatt gattcctctc ttgtgaaata aatcatgtat taaaatcatt cattatatgt
    31921 tctaattttc ttacgtgtta cgcaaataaa tgagaatcat tctcacatag aatactctcg
    31981 gtaattgaga atgtcaatta tacttgtgat attatgagcg aatctttagt attgatagat
    32041 aaaaatgaga aaaaaatatt tcttcatagt tttccacaat tttttctcta ttaaatttgc
    32101 tattttccca cacgatactt gttttcagct cttttttcaa aaacacaaaa cttagtattc
    32161 cacagtttcc acaacacaat atgtagtgtt tgttgtttga cttttttact aagagtttta
    32221 tactatctat tatagataat aagaatgtgt tagaggaggc actcaccatg aacgtaaaaa
    32281 tcttttctaa aaataattgt atgcaatgta agatggtcaa acggttttta acagaaaatc
    32341 aaattgcttt tgaagaagtc aatatcgatg aacagcctga agcagtagac tggttgaaag
    32401 cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc agaaacagtt atcggattcc
    32461 gtcctgatca gttgcgtaaa cttgccagct aacatgacta aaagaatcaa aacgtgatac
    32521 atggatttga agggcttgag acagcaccag tttttgtcgc aaacttccaa atcctttttt
    32581 aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca tcaaatacga aatgaaggtt
    32641 gggcgtacgt atgagtctta aaaatttaca agatgtcagt tatttcaagc tgaataatga
    32701 aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag gataaagaag ctttagttgc
    32761 cttttttgag gaaaatgtca aaccaaatac gatgactttt ctatctatta tggaaaaaat
    32821 caattacttg atcgatcaag aatatataga agcagaattc attcaattgt attcaccatc
    32881 atttattgaa aagctatatc aatttttgga cgagcaacat ttttctttca aatcttttat
    32941 ggctgcttac aaattttatt ctcaatatgc gttgaaaacc aacgacggtt ccgcttatct
    33001 ggaaacttat gaagatcgag tagccttcaa tgcgctttac tttgcgaacg gggatgaaga
    33061 attagcactt gctcttgcag acgaaatgat ccatcaacga tatcagccag ctacaccttc
    33121 tttcttgaat gctggacgta aacgtcgggg cgaattggtg tcttgtttct tgactcaagt
    33181 cactgacgat atgaactcta tcgggcgctc aatcaactct gctttgcaat tgtcccgtat
    33241 tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa gccggcgctc ctatcaaagg
    33301 atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa ttattcgaag atagtttcag
    33361 ctattctaat caattaggtc agcgtcaagg agctggcgtg gtttatttga acgttttcca
    33421 tccagacatt attgctttcc tttcagcaaa aaaagaaaat gcagatgaaa agattcgcgt
    33481 aaaaactctt tctcttggcg tgatcgtacc tgataaattt tatgaattga cacgtaaaaa
    33541 cgaagatatg tatttattta gtccatatag tgtcgaaaaa gaatatggtg tcccattctc
    33601 ttatgtaaat attacagctg agtatgatca tctagttgca aatccaaaca ttcgcaaaca
    33661 aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa ttacaacaag aatctggcta
    33721 tccttacgta atcaatattg atacagcgaa ccgcgagaat ccaatcgatg gcaagatcat
    33781 catgagtaat ttatgttcag agattttaca agttcaaacg ccttctgtga ttaacggcaa
    33841 acaggaatat gaagtattag gaacggatat ctcatgtaac ttaggctcga caaatatcgt
    33901 gaatttgatg caaagcccta actttggtca atctgtgcgt acgatgactc gtgcattgac
    33961 atttattaca gatgcatctg atatcgatgt cgtaccaccg atccaaaacg gaaataagct
    34021 gaatcatacg attggattag gtgccatggg cttacacact tatcttgcaa aagaacaaat
    34081 ggaatatggc tcagaggatt cattagactt tacaaatatt tacttcatgc tgttgaatta
    34141 ttggacactt gttgaaagca acaatatcgc tcgtgaaaga aaacaagttt tccataattt
    34201 tgaaaaatca gcttatgctg acggcactta tttcgaaaaa tacgtaactg gagaattcca
    34261 gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt tttatcccaa ctgctgaaga
    34321 ttggaatgct ttgaaacaag cagtaatgaa agatggactg tatcatcaaa atcgcttggc
    34381 agtagcacca aatggctcaa tttcatatat caacgatacg agtgcaagta ttcatcctat
    34441 cactcgcatg atcgaagaac gccaagagaa gaaaatcggt aaaatttatt acccagcgcc
    34501 ttatctatcc aatgagacga ttccgtatta cacttctgct tatgatatgg acatgcgtaa
    34561 agtaatcgat gtttatgcaa tggcacaaca acacgtcgat caaggcatga gtttgacttt
    34621 attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg aagacaacaa cgaagcaaac
    34681 gacacgtgat ttgaatattt taagacatta cgctttccac aaaggaatca aatccattta
    34741 ttacgtacgt acctttactg atgatgcaga agaaatcgga agcaatcaat gtgaaagctg
    34801 cgtgatctaa tatcatagat agattcgctt attcatattt taattaaagg agcattctca
    34861 tgtcagaaac atattatgaa gcaataaact ggaatgaaat tgaagatatc attgataaat
    34921 ccacttggga gaagctgaca gagcaatttt ggctagatac acgtatccca ctttcaaatg
    34981 acctagatga ttggcgtaca ttgtctcaat tggaaaaaga tacagtagga catgtatttg
    35041 gcggtttgac actgctagat actgtccaat ctgaaagtgg catggatcag ttgcgtaaag
    35101 atgtccgcac tccccatgaa gaagccgttt tgaacaatat tcaattcatg gaatccgttc
    35161 atgccaaaag ttattcttct atttttagta cactgaatac gaaaaaagaa atcgaagaaa
    35221 tttttgactg gacaaatacg aatccttatc tgcaaaaaaa agctgagcgt atcaatgaaa
    35281 tctacaaaca cggtacaccg ctagaaaaga aaattgccag cgtcttttta gaaacattct
    35341 tgttctattc aggtttttat actcctcttt attatttagg aaacaacaaa ttggctaacg
    35401 tagcagagat catcaaacta atcatccgag atgaatctgt acatggaacc tacatcggtt
    35461 acaaattcca attaggattc aacgagttac ctgaagcaga acaagaaacg ttaaaagact
    35521 ggatgtacaa tcttctttat gagctttacg aaaacgaaga acgttataca gaagaattat
    35581 acgatccaat cggctggaca gaagaagtca agacattcct acgctataat gccaacaaag
    35641 cattgatgaa cctagggatg gatccactct ttccagatac tgccaatgat gtcaacccaa
    35701 tcgtaatgaa cgggatttca acaggcacaa gcaaccatga cttcttctca caagtcggaa
    35761 acggctattt gttaggtcat gtagaagcga tgaaagacga cgattatttg attggattgg
    35821 aataaggatt tattttaatt caaaaatttt tcgaaaaata aaaagagttt agcgtacaga
    35881 tattttaaat gtacactgaa ctctttttta tgcttattca cttgtttctt ccagcacttt
    35941 tggactgtct aacttttccg aaacatgttc cattcttctc aataaataga ttttccggat
    36001 atttaatatt atcgtcttaa tcactgcata gacaggtatg caaatcagca tgcctaatat
    36061 tcctgcgaca cttcctgctc caatcaatac caagatgatc gtcaaaggat ggatattcat
    36121 tgatttccca aataacaacg gcttgatcaa atttccatct aattgttgga tcactaaaat
    36181 cgacaacgcc atatacaaag cttgaaatgg agagataaac aaaccgacaa tgattgctgg
    36241 taccgctcca ataaaagggc cgacatacgg gataatattc gtgattccgc aaatgaccgc
    36301 aagcaacaag ctataaggct gatcgaatat tctcattgcc aaaaagctca ttacaccaat
    36361 aatcaaagca tctaagatcg tactgctgat ataggcagat aatgtttcgt ttaattcttt
    36421 taccgtttgg cgtaactctt tacgaatcgc tttcggaaag aagtgagaca acgcatcaat
    36481 aaatttgtgc ccatccttga acataaatag caaaataaat ggaacggtaa ataacaaaat
    36541 aaaaaattgc atcataaatg aaatgatttt tgagacactt gcagataaac tgactaatat
    36601 gatattgaac aaattactaa gagataaatt tgctgatgct aattgttctt ccaaattgaa
    36661 atttttaaac tcttcccgct gcgccaattc atttaaccat ttgcttgttt catctgcata
    36721 cattggcaag ctctgggtca attgaattgt ctgttccatt aactgaggca ctacattcat
    36781 gacagttaaa acaactaaaa tgattacgac caaaaacgtc aataaaaaac cgaacactct
    36841 cgggatccga tgcttttcga gaaaaacaac gattggatcg aacatataat agataaaacc
    36901 agcaacaagc aatggtaaaa aaactgccga aagcattttc aatattggac taaaaatatt
    36961 cggcatttgt aaaataaagt acacgccgat gatcgtgagt actagctcca ctgtccagaa
    37021 aaataatttt gattgtttaa aacgtgattg catacatctc ctccaaggtc ctttgatact
    37081 tctattctat catttaacaa agagttacgc gctgagattt tcatttgctt aaaaaaaaca
    37141 aaaaaaataa acatatttta atgagtagac gagtacttaa acaaaaacac ggtttggagt
    37201 agtgacaaaa attatactgc agtccctttt tccaaataaa cggtgttcaa aagcttgcat
    37261 cttcgtaata agcccagcca aacgaaaaat ataaggaact atttttattt ggctgggctt
    37321 attacttgat gtaggacatc tattttacaa cctcagatca tttattgcgc aatactgact
    37381 aaatgcaact ttgtgatatt tttgtgtacg gacaactcct cgatcacatc ggcataagtg
    37441 agcccttttg gaagatcaat cgtatagata tttttgtaca ctcggtagtc atcaacgaat
    37501 tttacgtcaa aattcacatc ttcgattacg atatttttct cttcaaaata atcggtaaga
    37561 aaatctttgg tttcttttct atggataaat tggatttcta actttttagt cgtaggtaca
    37621 tggatcacgc gtttcactac tgtgagtgca atagcaatcc cgataaaact gatgatcgca
    37681 atggaatagt aacccatgcc gatggcgatc cctaatccag caattgccca taaagaagcg
    37741 gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga tcgttcccgc gcctaagaaa
    37801 ccgacaccac tcaccacttg agcaatcagc cttgcttcat ctgaacgaat cgttccagca
    37861 agctgtggat actctaaagc atctcttaat gcaccactag cgatctcaac ttggatcaat
    37921 gcaatgatac atgctcccat acaaactaaa atatgcgtac gcatacctgc tggacgattt
    37981 ttatattgtc gttcaaaacc gattgctccg ccaaatagca tagcgatccc taaacgtaaa
    38041 ataatctctg gaattgtcaa taccatatca ttcatctaaa caccctccag tccaatatat
    38101 catgtctgaa aggagcagac aaaaaaagtg caatttttac ctcttacgaa aaaaggactg
    38161 tgacaaaatt tttgtcatag tcctttttat gaataaacgg tgttcaatca gctgaccctc
    38221 ggtattactc aacaaacggc aaaatatggg gagctatttt tcctttgtct tctctaatac
    38281 tcaggtcaaa acgctgattt cacaacctct ttagcctact attgaaaatt acaacttact
    38341 tgctgcttct tcatcgatga tcacaacaac attgtcgtga ttttgcaata cacttgctgg
    38401 caaatgattc gttacaggtc cttcaatcat ccctttgatt gcatcagctt tgctttcgcc
    38461 aaatgccatc aagacgattt ctttactttt cataattgaa ccgattccca ttgaaacagc
    38521 ttttgttggt acatcttctg ctttatcgaa gaatcgtttg tttgcattga ttgttgattc
    38581 tgttaagtca actactgaag tcgttccgtc aaatgaagca ccaggttcat tgaaaccgat
    38641 atgtccattt tgcccgattc ctaagatttg gatatcgatt ggatgtgcat cgatgatttg
    38701 atcatacttt ttgcatgctg cttccagatc ttctgctttt ccatctggta caaacgtttc
    38761 tttgaatggt tttttatcaa ataattggac atccatgaaa tgacggtagc tttgttcatc
    38821 tgaaccgcct agtccaacgt actcatctaa gtttacagaa gtcatttcac tgaaatccaa
    38881 atcgcttgaa gtcatttctt tatataaagt aactggtgtg cttcctgttg ccaatcctaa
    38941 tacttttgca cctttgttca taccttcttt gatgatttca aatgcttttt ttccgccttc
    39001 ttcagcgttt tttacacgaa tgatttccat agtgattgct cctttatatt ttggtatagc
    39061 ccaattatag cattcttttt ttctttttac aataattagt atagaccaaa ttgcattttt
    39121 ttcattttct tgacaataaa taggaatccg tgttaggttt cataagatgg ttcaatcaaa
    39181 caatccatgt tgcatgaaaa ccaagcaagt gattcactgt ggacgcttta tgtctgcagt
    39241 ttttttatga aaaaggagtt attatgagta tactgacatt aaacaacatt acccagcaat
    39301 ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt aaatgctggc gaacatcttg
    39361 ggctgattgg acaaaacgga gccggtaaaa gtacattgat taaaatcatc actggtgaga
    39421 tcttaccaga tgacggacag atcaattggc aaaaaaatat tcacaaaggt tacctcgatc
    39481 aatatgtgga agtcgatgaa acattaacaa tcgaagaatt tctaaaaact gcgtacgcaa
    39541 aagaatacga aaaggaagaa aaaattgctt ttctttacca aacatatagc gagaccttcg
    39601 atgattcctt gctggaaaaa gctgggaaac tccaaacgga attagaccaa ggagtgtttt
    39661 atcaaatcga caccttagtt gctgagatga gcagcgggtt ggggatcgat gtcttaggtt
    39721 tagattcact gttgaaaaat ctaagtggtg gacaacgttc aaaggtgatt ttagcaaaac
    39781 tacttttaga aaacccagat gttctgatct tagatgaacc aaccaatcac ttagatgatc
    39841 agcatatcca atggctgacg cagtttcttc aggattttga cggtgcgtat atcgtgattt
    39901 cccatgacca agaatttctt gatcagatca ccacccatat cgcagacatc gagtttggaa
    39961 agattactaa gtatactggc catctaaaac aagcattgaa acaaaaggaa caaaatcgcg
    40021 aaagctattt gcgccaatat catgctcaac aaaaacaaat cgaaaagacc gaagcttata
    40081 ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa aagccgacaa aaacaattag
    40141 ataaaatcga acggctgacg cctccagctt cagcaagtaa agctgtcttt gattttccat
    40201 tctcgcctat tgtcacgaca ttagcagtgg aaacaactga tttagtcatc ggatatgatc
    40261 gaccgctact tgcacccatc aatttgacca tccgctttgg tgaaaatatt gccattcgag
    40321 gcttcaacgg gattggaaaa tctactttat taaaaacact gatcggtgag attgaaaaaa
    40381 taagtggcga tttccacttc ccagataaca caaaaatcaa ttacttttca caagatctgt
    40441 actgggaaaa tccattagaa acaccactac aatatttaag cagtcaattt ccaaaagcga
    40501 ctatcaaaga attacgtcgc caactagcta aagcagggtt agttaatcaa ttagcaagtg
    40561 aacctctcac tacattaagt ggtggggaac aaactaaggt gaaacttgca cagttgacaa
    40621 tgaaccaagg taatttactg attctcgatg aaccgacgaa ccatatcgat caagaaacga
    40681 aagaaagctt acaagacggg atacagaaat ttccaggaac agtaatcatc gtctctcatg
    40741 aacaagaatt ttatcaagat cttgtgcaac gagtcattga gattgaacca aaataaacgt
    40801 ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt ttgtcacaac tccttcttct
    40861 gaataaatgg tggccaatta gctgaccttc ggtattagct caaaaaacgg caaaatatga
    40921 ggagctattt ttatttggct attcttatta cttgatgcag aacagctttt cacaacctct
    40981 tttcgtctat tttttatttt cgagatttct ctttccttta aaaacaactg tttgaaagaa
    41041 gaacgcacat ccttgaaaaa caagcccgct aaccgttaca cctaaccagc catactgctg
    41101 ccacattgtc gtccctagca atgaaccagc cgcacctcca ataaagtaca taaacataaa
    41161 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
    41221 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
    41281 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
    41341 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
    41401 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
    41461 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
    41521 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
    41581 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
    41641 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
    41701 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
    41761 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
    41821 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
    41881 aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctgc
    41941 cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa
    42001 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
    42061 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
    42121 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtt ttttttcatt
    42181 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
    42241 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
    42301 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
    42361 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
    42421 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
    42481 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
    42541 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
    42601 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
    42661 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
    42721 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
    42781 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
    42841 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
    42901 taccagtatc agaatgattc ccaacgagat caatatcaat cggattattt tctttttcat
    42961 atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag
    43021 caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt taacagttac
    43081 agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt tagatacgcc
    43141 ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca agcctaaacc
    43201 agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg cagttaattc
    43261 tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag ctacagctac
    43321 aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt cgttaagttc
    43381 taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg ccattataat
    43441 ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa gctgcatctc
    43501 catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact ggcgcttgta
    43561 gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc aatgctgtga
    43621 tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct aatgcttttg
    43681 cttctttagc aaattcgtcc atgattttcg ctggtgcgac tacatcttcg ttactgaatg
    43741 caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct tttttagctg
    43801 cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca cgaagttgtt
    43861 tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact actactgatg
    43921 cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt gcgataattg
    43981 cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt tgcactgaaa
    44041 aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc tattcacttc
    44101 gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt gctcaaccag
    44161 tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac tctatgccac
    44221 cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg aaattaaggc
    44281 ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag cttagcatgc
    44341 taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct aatatttaat
    44401 tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat cgttacattt
    44461 ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc atggattgct
    44521 gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg tacatggata
    44581 tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt tactgctttt
    44641 gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt tggtcctaat
    44701 actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac tacatcaaag
    44761 tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa atctgcacca
    44821 gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta
    44881 ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt tttagggtct
    44941 acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt
    45001 gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt ttttaatgca
    45061 tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg gttataacgg
    45121 ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag ctgctgtctt
    45181 agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt caaccatgcg
    45241 cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag cgatttctct
    45301 tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg aacctttttc
    45361 gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa cgaatgtgaa
    45421 tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt gatcagctgt
    45481 acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac ctagtgcagg
    45541 acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa cgattttttc
    45601 tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat tggagatgca
    45661 tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat gtgcaaaaca
    45721 atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt tctgctattt
    45781 ttacattttg cttgaataaa cgctaaattt attctaaaaa aacaacaatt tttcgttctc
    45841 atttcttctt ttatgttaca cttgcgctag catggttcaa cagatgaaag caaacaataa
    45901 cacggttttt tagacccttg tacaaagatt cctacataac aaaatgaggt ggagaaaatg
    45961 aaagatttaa ggtatagaag tgtctttgat attattggac ctgtgatgat tggtccaagt
    46021 agctctcaca ctgccggagc tgctagaatc gggaaaatca tacgtagtat atttggcgaa
    46081 cagcctgatt ctgtagatat ttatctatat gaatcatttg caaaaaccta tcgggggcac
    46141 ggaacagata ttgcattagt tggcggcctg ttagggatgg aacccgatga tgaacgttta
    46201 gctgattctt tgaaattggc atatgaagcc ggtatggaag tcttgtttgt tcctaagagt
    46261 gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt
    46321 tcagtcactg gcatttcgat cggcggtggc aatatccaga tttctgaatt gaatggattc
    46381 aaaatttctt taagcatggg tactcctacc ttgatcgttg tccaccaaga tgttccaggt
    46441 atgattgcta aagtgactag tatactttct gagaccaata cgaatatcgg cactatgaca
    46501 gtgacacggg aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa
    46561 gtagaagaaa cggtaaaaag actcaaacag ctgccaaata tcgacagcgt aaattatttc
    46621 gattaaggag ctaaaaaaat gtttttatcg attgaagaat tagtagatca agcaaaagct
    46681 tatccaagtg tagcagaatt gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt
    46741 agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag
    46801 ggaaaagaag gtgttacctc cgtcacagga attaccggtg gtgatgcaaa aaaattagat
    46861 gattatttaa atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat
    46921 gctgtagctg ttaatgaagt caatgcaaaa atggggttga tttgtgctac accgactgcc
    46981 ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc gagatcggct tgatttgaca
    47041 catgaacagc aattggaatt cttatttacc gctggcgctt tcgggttagt catcgcaaac
    47101 aacgcttcta tcagtggtgc agaaggggga tgtcaagcag aagttggttc tgcaagtgca
    47161 atggcagcag cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca
    47221 gtagccatta cattgaaaaa tatgatggga ctgatttgcg atcccgttgc aggcttagtt
    47281 gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc aagcttttat ctctgctgat
    47341 atggcattag cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac
    47401 caagtcggac gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg cttagctgtt
    47461 acgccaactg ctaaacgact tgcggaagag attttaaata agagggcgtg ataaagctcc
    47521 ttaatttcga gtattaagag attagttatc ttcataacaa aaaacgaaga gaggactaat
    47581 tgcccttctc ttcgtttttt attaatccat caatttcgta ttttttactt catcgatcgt
    47641 ttttgttcct gcaagttgca tcgtgatcga caattctttg ttcaggtgtt caaaaacaga
    47701 tgttactcct tctgccccac ctagattcaa cccataaatc actggtcggc caatcgcaac
    47761 taagtcagca ccacttgcca gagctttgaa gacatgctct cctcgacgga caccagagtc
    47821 gaaaatcact ggtacacgtt tgttgactac ttcggcaatt tttggcaata cttcaaagga
    47881 cgcaggacct ccatctagtt gacgtccacc atgattggat acccagatac catctgcccc
    47941 tgcagagata gcgattactg catcttctgg cgattgtatt ccttttacga taactggaag
    48001 atgggtgatt tctttgattg ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc
    48061 ataaatttct gcgattcctt ttccttcgcc attaccactt gcactttgtt cgctatacgc
    48121 agctaagtta ggcattggta atggaaattg gaattgattg atgacgtctt cttcacggta
    48181 accaccgagc gttgaatctg ccgttaaaat gatagcttta gcccctgctt ctactgcttt
    48241 gttcaaaatg aactcattga atctgtcgtc tttactcata tataattgga aaaactgcgg
    48301 tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag tgctgatgga
    48361 aaagattgat ccagcagcag ctactccttt agctgtatca gcttcccctt tttcatgtgc
    48421 caatccttgg gccgctgatg gcgcttggat aatcggtgta tctaattcaa ttccgaaaac
    48481 agatgtatgc aaatcggctg agtcgattcc gcgtaatatc cgtggcatga ttttctttgt
    48541 attaaaagaa gtcgtatttt ctttcatcgt ccactcatct tcagaaccac cgcgaatata
    48601 gccaaaagct cctgcttcca tgcgctcttt tacacgacct tctagttagg caatgtttac
    48661 aatatcgatt ggatgttctt cattacttgt atgataagtc atagtagatc cttctttcat
    48721 cttcttgatt acatataaat catactaaac ttacaaaaag taagcaaaca tttactctca
    48781 gatactttta tcgctattct ttccaaataa atcgacttac aagctgatct acttcttggt
    48841 tttcatgaag catactatga gaggcatttt cacctgtcac tactttttcc tctatttcat
    48901 ttccgcgttg cttcaacaac gcatagaccg ctaatgcact gtttaacggt actgttccat
    48961 cactcaagtc tgtttcatct agttgaccag caatcaagaa aaatcgagtg gttataggaa
    49021 tattaccgat catttgctgg taatcttgat agcggttaga aacgacagca ggcccctttg
    49081 ctaactcatc tgtcagagac tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa
    49141 ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg tccatacgtt cctaaatagc
    49201 gtagtgcact aactcctccc atggaatgcc ccactatgtt gactttatct atgccgtagt
    49261 tttctttcag atacaaaaga caagttttga tccattctgc ttgattccac tcgttatttt
    49321 tattatcttc aaacaatact tggaccatcg gattgtctgc ttttccagac aaatctcctg
    49381 ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa ttcttttact gcttgctgat
    49441 ttttttccaa acgccgcaac atccctttaa aggaattttc tgtcccgctg tagccgtgga
    49501 aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc gttttttctt
    49561 tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac gcaaacaaac
    49621 tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt ggttctgtca
    49681 tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc atatgatcga
    49741 catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag ttctgtactg
    49801 gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc gtcaacttct
    49861 gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac ttcgccaact
    49921 tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat atgattgact
    49981 tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc
    50041 acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat ttcaacaagt
    50101 acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt gacttcagtt
    50161 tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat actttgcgca
    50221 cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg caagacatac
    50281 cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat aaaaaaacct
    50341 cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt tgtggctagc
    50401 gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta cgtaaaacat
    50461 caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga gttgttttct
    50521 gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt tcatgagaca
    50581 acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt acaatattta
    50641 caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga ttttgtgtag
    50701 ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat ttacttctgc
    50761 ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt ttacttgtca
    50821 atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta atttgaaatt
    50881 aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg tatataagaa
    50941 aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa aaattttaga
    51001 aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt tccgtggaaa
    51061 catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga cgcttcctgt
    51121 cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt tcttcaaagt
    51181 attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa atcattttta
    51241 tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga gcgtagaaca
    51301 aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg ctgctattgc
    51361 aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata tcgaaaaaga
    51421 cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga aagtatgtga
    51481 aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg aaaatttgca
    51541 aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct ttcttttttc
    51601 gctttttata attagacacg cttttcaaaa tcaagcgcgc gatctaaata atacatcaat
    51661 ggtgctgaaa cagccatgac gataaactca cttaatgcaa gcgtgccata ggttgtccaa
    51721 aatgcttggc cacctgttgg aactaacata taagcaatca gtcccatgct gatggtaaag
    51781 aagatcgtgt tcaatactaa acgcatcttt acatttttca ctttgttttg caataaagct
    51841 gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat gataccaaaa
    51901 ccaaagaagg cattatagac gatgaccccg cctaaaactc cccaaagcaa tttacggtta
    51961 aagacaacta aatgattcaa actttctgaa atacgaaact ggatcggccc tgaggataca
    52021 ggcgcaacta aaatagttaa cgctagataa agagccatga tgatgccatt tacaacgatt
    52081 acggataacc gttgtctgct tttcgttttt tgcattttct ctcctctttt ctcctagttt
    52141 ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa ttataacaga
    52201 cttttcattt atttcaatcc gtcttatctg cttttaaaaa agcaaaagct taaacaatgt
    52261 aaacgtttgc taaaaatcgt gggatatggt ataaagtaag tgtaaggggt cataagaatt
    52321 cccagtaaag cagacgaagt aggatctgca agaacagaat tgttgtgaaa cttaaataaa
    52381 taaaaaagga tgtgggaatt tgagcaacct cttactagag aacggtctag caagcagctg
    52441 aagatctaag tggacatgcc gaaccttgga acttctcctt acgtaagtaa tggatcatgc
    52501 gcttgctagg cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct
    52561 gctataataa ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca
    52621 ttcaaaaaat agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat
    52681 atcttgtttt attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa
    52741 tccatcggca ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa
    52801 aaataagcat attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat
    52861 tgctgaaact ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat
    52921 cgatactttc ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga
    52981 tactttctat cagatggaag tagaattccc agttgcttat attccagcag gttctggaga
    53041 tgattttgct cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt
    53101 agcagctcaa tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa
    53161 aaaaggaata gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac
    53221 aaatcattct tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt
    53281 attctctatt ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg
    53341 aggaaaacaa ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt
    53401 tggcggagga atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt
    53461 cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa
    53521 aaaacaattg aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc
    53581 aacaacaccg caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat
    53641 cacaataacc acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct
    53701 acatctggaa caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct
    53761 ttttttacta tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt
    53821 acccaaaata aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt
    53881 gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt
    53941 attttgaaca aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa
    54001 gtattctgct ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt
    54061 gatcccaccg ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat
    54121 gatcggcaat aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga
    54181 ggtgtggatc actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta
    54241 actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg
    54301 gtttcgttta ctgatctctt tgcgattaaa tgctagattg atgacgctga aaccgctaaa
    54361 aatggataga aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt
    54421 catccgctga ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg
    54481 tgtctgttcc atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg
    54541 caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg
    54601 tatcttgatc gtcggcatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg
    54661 taagataatg tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc
    54721 atccaatgta acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg
    54781 cgactcttct tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc
    54841 aatattttgc gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt
    54901 cgatttattt gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc
    54961 tttccatcca agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat
    55021 tggtataaaa actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca
    55081 agttgacgag atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt
    55141 gttccgaccc catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc
    55201 tatccttttg caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa
    55261 tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt
    55321 ctacttgata gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg
    55381 taaagcttgc tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca
    55441 agcgaatacc atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca
    55501 tcgtcccgta gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca
    55561 tattacgacc tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg
    55621 gcgcgagatc aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta
    55681 aaaattgatc catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga
    55741 gcgaaacaga tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt
    55801 cattcgcttc tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga
    55861 cttgttctaa gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa
    55921 ttccagtatt aatcgtttcg agattgccac cttgatcaat ctcgccaaag gccgccataa
    55981 acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca
    56041 gcagtatttt tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct
    56101 ctcaaatcct tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct
    56161 atttttccgt aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta
    56221 tttagttcag tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct
    56281 attgttcgct gacaacacgt tttctgggag taatgggatg tcgatcgtca aacgatccgc
    56341 cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct gtccttgatc
    56401 catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg ttgtatagac
    56461 aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat tccgactttg
    56521 ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt gggcaatccc
    56581 acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac gaaacgaaac
    56641 atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc ttttctcttt
    56701 ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta gttcgtcaaa
    56761 gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat tttcaggagt
    56821 cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag aaagcataca
    56881 gttgatggca gtcgatttcc cactgccgtt tggtcctaac agacctaaga cttctccttc
    56941 ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt tgactaattt
    57001 atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat ctgactataa
    57061 taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca tttgtcacaa
    57121 tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt ctactggtta
    57181 tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc tatttattga
    57241 tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat tggattttcc
    57301 aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt ctgccagcga
    57361 cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg ttaggttttt
    57421 tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc gtaattttgc
    57481 tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt gagaaaaagt
    57541 acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa caaaataaag
    57601 cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa cggaaccgaa
    57661 ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt ttacaaattg
    57721 gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa aatttaagtg
    57781 ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc catgagacat
    57841 cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct tctgttacga
    57901 ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc ttaatggtca
    57961 tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt gttttacatt
    58021 ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc agcccacaaa
    58081 aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa gaacaaaagg
    58141 gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt ttaccaaggg
    58201 agggacacca atgataacag tagtcattat tgagatgatc catttgtgac gaaatcgtta
    58261 caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca ttgtgccaag
    58321 gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    58381 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    58441 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    58501 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    58561 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    58621 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    58681 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    58741 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    58801 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    58861 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    58921 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    58981 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    59041 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    59101 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    59161 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    59221 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    59281 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    59341 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    59401 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    59461 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    59521 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    59581 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    59641 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    59701 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    59761 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    59821 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    59881 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    59941 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    60001 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    60061 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    60121 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    60181 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    60241 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    60301 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    60361 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    60421 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    60481 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    60541 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    60601 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    60661 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    60721 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    60781 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    60841 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    60901 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    60961 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    61021 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    61081 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    61141 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    61201 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    61261 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    61321 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    61381 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    61441 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    61501 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    61561 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    61621 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    61681 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    61741 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    61801 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    61861 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    61921 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    61981 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    62041 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    62101 agtgggccat ctttatgttg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    62161 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    62221 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    62281 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    62341 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    62401 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    62461 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    62521 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    62581 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    62641 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    62701 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    62761 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    62821 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    62881 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    62941 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    63001 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    63061 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    63121 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    63181 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    63241 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    63301 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    63361 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    63421 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    63481 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    63541 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    63601 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    63661 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    63721 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    63781 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    63841 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    63901 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    63961 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    64021 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    64081 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    64141 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    64201 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    64261 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    64321 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    64381 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    64441 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    64501 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    64561 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    64621 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    64681 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    64741 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    64801 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    64861 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    64921 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    64981 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    65041 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    65101 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    65161 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    65221 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    65281 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    65341 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    65401 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    65461 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    65521 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    65581 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    65641 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    65701 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    65761 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    65821 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    65881 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    65941 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    66001 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    66061 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    66121 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    66181 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    66241 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    66301 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    66361 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    66421 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    66481 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    66541 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    66601 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    66661 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    66721 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    66781 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    66841 ctttgcaaaa agcatcaaac cagatgtatc ccgatctagt cgcgtcacca catggattac
    66901 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    66961 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    67021 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    67081 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    67141 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    67201 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    67261 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    67321 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    67381 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    67441 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    67501 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    67561 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    67621 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    67681 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    67741 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    67801 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    67861 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    67921 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    67981 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    68041 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    68101 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    68161 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    68221 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    68281 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    68341 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    68401 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    68461 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    68521 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    68581 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    68641 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    68701 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    68761 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    68821 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    68881 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    68941 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    69001 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    69061 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    69121 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    69181 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    69241 accggaaagc ttacaacaaa acgggctgaa ttccctattg aagaaggact tttagctatt
    69301 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    69361 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    69421 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    69481 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    69541 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    69601 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    69661 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    69721 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    69781 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    69841 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    69901 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    69961 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    70021 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    70081 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    70141 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    70201 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
    70261 aatacgtcga ctcccttaca agagagccga cgtatttttt tcgtttctat tcgagaaaat
    70321 caaaaataga aagccttata aacgatcgat caattcttct aattcattta gacgttgttc
    70381 aaacatcgac attgcatctt caatatatgc agcctgtgtc atatcaactc ctgctttttt
    70441 cattacttcc acaggataat cgctgttacc tgcttttagg taagccaagt agttctctag
    70501 tgcctctggt tcctggttta gtattttctt cgccagtgct gaagcagcag aaaagccagt
    70561 cgagtattgg aaaacatagt aattataata aaaatgcggt atccgtgacc attcgaattt
    70621 aatttctggg tcttcttcga ctgctggacc ataatatttt gcattcaatt taccataact
    70681 atcacttagg tattcactag tcaatggcac acctttttca tcttccgtat gcatgaaatg
    70741 ctcgaattca gcgaattgtg tctggcggaa aactgttcct ttaaacccat ccaagtaatg
    70801 gttgagtaca taagcccgta cacgagggtc tttttctgtt tccaataaat actccgttag
    70861 gatattttca ttcgttgtcg aagcaatttc tgctaaaaag atggagtagt cgccgtacac
    70921 ataaggctgg ttcgaacgag tgaaataact atgaacacta tgtcccattt cgtggacaag
    70981 cgtaaatagc tgatccagcg tatcatgcca attcaataaa atatatggat ttgtgtcata
    71041 gcttcccgaa gaataagcac cgcttcgttt ccctttattt tcgacaacat cgatccaacg
    71101 ttcagagaat gctttttcta cgatggccat gtattcttca cccattggtt tcagtgcttc
    71161 taaagctttt tcttttgctt cttcgtacgt aaaggtaatt ggagcttccc ctaagaccgg
    71221 tgtataaaga tcatacatgt gcagtttttc cacttctaat aaacgtttcc gtaattccat
    71281 gtatcgatgt aacaaaggca aatgcttgtt taccacgtct accaaagtat cgtatacact
    71341 ttcaggaata tgattattgc tcaaagacgc ttctctggca gagctgtaat tacggacttt
    71401 cgctttaaaa ttatgtcctt ttatatgtgt gcctaaagta gaagcaaatg tatttctaaa
    71461 ttgttcgtaa acactgtaca atcccttaaa tgctgcttcg cgcacccttc gatccgtgct
    71521 ttctagcaac tgaccataca cgccatgaga taattggact atttcaccat tttctccttc
    71581 aatcgttgga aaaactagat ctgcattatt caaaacagca aatgtatcac ttgaagcatc
    71641 aaagatttca cctgctccag caagaagaga ttcctgctca gcagataaga catgggctcg
    71701 attatctact atttgctgga tataatggcg atagacttcc aattttggtt cttctttgaa
    71761 atattgccaa atctggtcat ctgacaattg caatatttct ggttcaaacc aggaaacggc
    71821 ttcactaact ttcgaaaaca gactgcttgc tcttgcataa agcgcttggt aatctgtatt
    71881 tccagtgtct tgatcgtttt taaggtgcgc atatacataa atgacttcag tttggcgata
    71941 aaccctcaat acgaattcaa tcgcatttaa aaattgagaa gcgccttgat caagtgttcc
    72001 tttgtgtttt tcagattgtt ttaactcttc tgataattcc aaatatttct catcaaactc
    72061 ttggtcgctt gaaaagatct tggtcaagtc ccaagttaaa ttttcaggca attcttctcg
    72121 ttttggcaac tgctttactt ccatgaaatc cactcctttt cttcccttag ctctatctat
    72181 caaaatagat catagtctaa catccacatg tcacagcatc taacttttct actcattcat
    72241 gtgtctgctt ccatcaattt tagcataact aaatcttgtt ttacatagaa aaccgattcc
    72301 tagcctattt ttatacttac tattttttca aagaagacaa gtgtatgcat tcgttaaaaa
    72361 actgctgata gaccatctct tcatcaatca taggaaaaag ccattccctt ttatgttctt
    72421 ttatttggca aagccatgag ttgaaatcag gaactttctg gttttccaag gcttcttcgt
    72481 acaacattcg ataaagaaaa acctgttctt gtaaataaaa gaaaaaccga cttttttgat
    72541 agatccacga tgatagatgc aaaagatgtt tatgacgcaa gtagcattgt tcttgtatgg
    72601 atactgtatt cttttgcttg tgaataagtt ggcgtgtcag ccatttcttc ccatcttcac
    72661 tcactttttc tatttttttc atttggttca ttttaatttc gttactagtg aatatttctt
    72721 taaggtccct actagaaaaa gtccagcagc tactgcaaaa ggaaatatgc cctgatacag
    72781 tctctgtaat atgatgatac aaataaagct tttgtttctt gagatcagct ttccagcaat
    72841 gcactcctct ttttctatta tagtagcagc aacttttttc tatcaatgag aattgcgatt
    72901 gtccaagaaa agaattacca agtatccacc atacttggta actgttattc aaataatttt
    72961 gggttctttc aataaatctc tggtgagaca gtctagaaca ttggatttct attgctagat
    73021 tcgccaacaa aatatccggc ctttgccgca acctcggcaa ataggcttcc aaaaatactg
    73081 gttctactga ctgtcccagc caatccatga agcattcctt taactgtaag tgctcttttg
    73141 attctctttc actaaagacc gaacagttat ctgatttttg atgggcaaaa tgagcacatt
    73201 ttttctttcc ctttttcaaa atcactcttt ttcgacagcc acgacaataa tatttctttc
    73261 ctttttctgc atgtttagca aaacagcact tattatattc attcattgca agcagcattt
    73321 ttctccctct gaacttattt acgtctaaga atttatttac acgaaaaaag agggcgtgaa
    73381 aaagctattc tgcatcaagt aataagaaca gctaaacaaa aatagttccc aatatttttc
    73441 gtttggttgg gcttgttacc gcagatgcaa gcttttgaac acatttatac gaaaaaaaga
    73501 ctgcgacaca atttttgtca cagtctctta ctataatgct gatttataca aaataaaatt
    73561 tcgttcgctc gatagcatta cgcgccatca gacattgtcc atattcattc aatgtttctg
    73621 gtgtacgatc gctcttttca gcaaactcca aaattcgagc aatctgattc tctacatccg
    73681 cttggttgag attttccatc cagaagtgaa cgactaggta atagcgctct tcgaataaat
    73741 acaaatcagt ccaagcagac tctagttcga catctgttgc aagttggatc acttgttcaa
    73801 aatcttcaaa agcaaaagtt gcttgtcttt cctgttcttc tagggcgctt ccagataatt
    73861 cttcctccgg atcgctgcca ataatctgct gacgaagaaa gtcactcaca tcttctgaat
    73921 caacagtaga atcttctgat tgagaaattt cttcatccgg aagattttta ctgataaaca
    73981 gttctaatcc gtctcctttt ggcaaaactt ggaaagtgac agcttcgctt cctcggaact
    74041 cctcttcgat atcaacttct tccaaaatac tataaaagaa attttcgatt tctttgtgat
    74101 tgccaagtaa atccaaaaat gaaacgcccc gttcctctaa atcttcatga ccaattagta
    74161 cacggatcgt attttcatta atatgttcca tttccatttt gctacacctc actttgccac
    74221 aatgtttaat atagattcat tgtagcgaaa tcagcttaaa tgtaaagaaa aaaaatctcg
    74281 ctgttaagcg aatttaataa aaccagcgga tttttcaacc tttcaagcta tcttatttaa
    74341 ctataacatt tttggcagac gaatactata tagccgactt ttcagctaat tttccctctc
    74401 tatttttcgt gcaatcatca tcttctacta cctttttata catctaatca agaatacttt
    74461 ttgtaaaaac aaaaaaactt ggaaaaacta tctttgtaat agt
//
LOCUS       DACEMS010000002        73611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEMS010000002 DACEMS010000000
VERSION     DACEMS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.002"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            510..1700
                     /locus_tag="IW735_000077"
     CDS             510..1700
                     /locus_tag="IW735_000077"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1043109.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            1802..2755
                     /locus_tag="IW735_000078"
     CDS             1802..2755
                     /locus_tag="IW735_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1043110.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            2824..4173
                     /locus_tag="IW735_000079"
     CDS             2824..4173
                     /locus_tag="IW735_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1043111.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(4356..4679)
                     /locus_tag="IW735_000080"
     CDS             complement(4356..4679)
                     /locus_tag="IW735_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043112.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            4808..5971
                     /locus_tag="IW735_000081"
     CDS             4808..5971
                     /locus_tag="IW735_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043113.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            6041..7390
                     /locus_tag="IW735_000082"
     CDS             6041..7390
                     /locus_tag="IW735_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1043114.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7435..7866)
                     /locus_tag="IW735_000083"
     CDS             complement(7435..7866)
                     /locus_tag="IW735_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1043115.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            8254..10896
                     /gene="alaS"
                     /locus_tag="IW735_000084"
     CDS             8254..10896
                     /gene="alaS"
                     /locus_tag="IW735_000084"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1043116.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            11096..11692
                     /locus_tag="IW735_000085"
     CDS             11096..11692
                     /locus_tag="IW735_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1043117.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            11814..12962
                     /locus_tag="IW735_000086"
     CDS             11814..12962
                     /locus_tag="IW735_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1043118.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(13000..13413)
                     /locus_tag="IW735_000087"
     CDS             complement(13000..13413)
                     /locus_tag="IW735_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1043119.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            13492..13923
                     /locus_tag="IW735_000088"
     CDS             13492..13923
                     /locus_tag="IW735_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1043120.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(14022..14255)
                     /locus_tag="IW735_000089"
     CDS             complement(14022..14255)
                     /locus_tag="IW735_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1043121.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            14438..16108
                     /locus_tag="IW735_000090"
     CDS             14438..16108
                     /locus_tag="IW735_000090"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1043122.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16255..16641
                     /locus_tag="IW735_000091"
     CDS             16255..16641
                     /locus_tag="IW735_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043123.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            16628..17326
                     /locus_tag="IW735_000092"
     CDS             16628..17326
                     /locus_tag="IW735_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1043124.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            17331..17795
                     /locus_tag="IW735_000093"
     CDS             17331..17795
                     /locus_tag="IW735_000093"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1043125.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            17792..18703
                     /locus_tag="IW735_000094"
     CDS             17792..18703
                     /locus_tag="IW735_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1043126.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            18938..19477
                     /gene="pyrR"
                     /locus_tag="IW735_000095"
     CDS             18938..19477
                     /gene="pyrR"
                     /locus_tag="IW735_000095"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1043127.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            19491..20774
                     /locus_tag="IW735_000096"
     CDS             19491..20774
                     /locus_tag="IW735_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1043128.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            20787..21713
                     /locus_tag="IW735_000097"
     CDS             20787..21713
                     /locus_tag="IW735_000097"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1043129.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            21732..23012
                     /locus_tag="IW735_000098"
     CDS             21732..23012
                     /locus_tag="IW735_000098"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1043130.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            23017..24093
                     /gene="carA"
                     /locus_tag="IW735_000099"
     CDS             23017..24093
                     /gene="carA"
                     /locus_tag="IW735_000099"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1043131.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            24098..27283
                     /gene="carB"
                     /locus_tag="IW735_000100"
     CDS             24098..27283
                     /gene="carB"
                     /locus_tag="IW735_000100"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1043132.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            27337..28113
                     /locus_tag="IW735_000101"
     CDS             27337..28113
                     /locus_tag="IW735_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1043133.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            28110..29042
                     /locus_tag="IW735_000102"
     CDS             28110..29042
                     /locus_tag="IW735_000102"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1043134.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            29039..29755
                     /gene="pyrF"
                     /locus_tag="IW735_000103"
     CDS             29039..29755
                     /gene="pyrF"
                     /locus_tag="IW735_000103"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1043135.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            29762..30391
                     /locus_tag="IW735_000104"
     CDS             29762..30391
                     /locus_tag="IW735_000104"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1043136.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            30379..30693
                     /locus_tag="IW735_000105"
     CDS             30379..30693
                     /locus_tag="IW735_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043137.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            30731..31372
                     /locus_tag="IW735_000106"
     CDS             30731..31372
                     /locus_tag="IW735_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1043138.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            31403..32107
                     /locus_tag="IW735_000107"
     CDS             31403..32107
                     /locus_tag="IW735_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1043139.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            32104..33006
                     /locus_tag="IW735_000108"
     CDS             32104..33006
                     /locus_tag="IW735_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1043140.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(33164..34870)
                     /locus_tag="IW735_000109"
     CDS             complement(33164..34870)
                     /locus_tag="IW735_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1043141.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            35060..35683
                     /locus_tag="IW735_000110"
     CDS             35060..35683
                     /locus_tag="IW735_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043142.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            36133..37182
                     /locus_tag="IW735_000111"
     CDS             36133..37182
                     /locus_tag="IW735_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1043143.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            37179..38060
                     /locus_tag="IW735_000112"
     CDS             37179..38060
                     /locus_tag="IW735_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1043144.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            38063..38833
                     /locus_tag="IW735_000113"
     CDS             38063..38833
                     /locus_tag="IW735_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1043145.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            39112..39966
                     /gene="aroE"
                     /locus_tag="IW735_000114"
     CDS             39112..39966
                     /gene="aroE"
                     /locus_tag="IW735_000114"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1043146.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            40144..41307
                     /locus_tag="IW735_000115"
     CDS             40144..41307
                     /locus_tag="IW735_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1043147.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            41424..41834
                     /locus_tag="IW735_000116"
     CDS             41424..41834
                     /locus_tag="IW735_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1043148.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            42008..44236
                     /gene="topB"
                     /locus_tag="IW735_000117"
     CDS             42008..44236
                     /gene="topB"
                     /locus_tag="IW735_000117"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1043149.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            44374..44691
                     /locus_tag="IW735_000118"
     CDS             44374..44691
                     /locus_tag="IW735_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043150.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            44851..45087
                     /locus_tag="IW735_000119"
     CDS             44851..45087
                     /locus_tag="IW735_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1043151.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            45084..45482
                     /locus_tag="IW735_000120"
     CDS             45084..45482
                     /locus_tag="IW735_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1043152.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(46149..47093)
                     /locus_tag="IW735_000121"
     CDS             complement(46149..47093)
                     /locus_tag="IW735_000121"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1043153.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(47389..48333)
                     /locus_tag="IW735_000122"
     CDS             complement(47389..48333)
                     /locus_tag="IW735_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1043154.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            48554..49768
                     /locus_tag="IW735_000123"
     CDS             48554..49768
                     /locus_tag="IW735_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1043155.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            49790..51805
                     /locus_tag="IW735_000124"
     CDS             49790..51805
                     /locus_tag="IW735_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1043156.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(51888..53261)
                     /locus_tag="IW735_000125"
     CDS             complement(51888..53261)
                     /locus_tag="IW735_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1043157.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(53287..53571)
                     /locus_tag="IW735_000126"
     CDS             complement(53287..53571)
                     /locus_tag="IW735_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1043158.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(53573..55588)
                     /locus_tag="IW735_000127"
     CDS             complement(53573..55588)
                     /locus_tag="IW735_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1043159.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(55767..56072)
                     /locus_tag="IW735_000128"
     CDS             complement(55767..56072)
                     /locus_tag="IW735_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1043160.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(56161..57192)
                     /locus_tag="IW735_000129"
     CDS             complement(56161..57192)
                     /locus_tag="IW735_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1043161.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            57399..58274
                     /locus_tag="IW735_000130"
     CDS             57399..58274
                     /locus_tag="IW735_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1043162.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(58426..59268)
                     /locus_tag="IW735_000131"
     CDS             complement(58426..59268)
                     /locus_tag="IW735_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1043163.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(59360..61237)
                     /locus_tag="IW735_000132"
     CDS             complement(59360..61237)
                     /locus_tag="IW735_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1043164.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(61234..62655)
                     /locus_tag="IW735_000133"
     CDS             complement(61234..62655)
                     /locus_tag="IW735_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1043165.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(62658..66047)
                     /locus_tag="IW735_000134"
     CDS             complement(62658..66047)
                     /locus_tag="IW735_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1043166.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            66304..67695
                     /locus_tag="IW735_000135"
     CDS             66304..67695
                     /locus_tag="IW735_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1043167.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(67768..68415)
                     /locus_tag="IW735_000136"
     CDS             complement(67768..68415)
                     /locus_tag="IW735_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1043168.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(68423..69109)
                     /locus_tag="IW735_000137"
     CDS             complement(68423..69109)
                     /locus_tag="IW735_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043169.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(69115..70188)
                     /locus_tag="IW735_000138"
     CDS             complement(69115..70188)
                     /locus_tag="IW735_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1043170.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            70410..71084
                     /locus_tag="IW735_000139"
     CDS             70410..71084
                     /locus_tag="IW735_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1043171.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            71089..72429
                     /locus_tag="IW735_000140"
     CDS             71089..72429
                     /locus_tag="IW735_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1043172.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(72578..73564)
                     /locus_tag="IW735_000141"
     CDS             complement(72578..73564)
                     /locus_tag="IW735_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1043173.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        23142 a        13172 c        14966 g        22331 t
ORIGIN      
        1 atagcttctc gtgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc
       61 gtttattcgg aagaatagga ctgtgacaag acttttgtca cagtcctttt ttcgaattca
      121 acaagtaaaa cttttaagaa atattcagag agtaaaatta aaaattgctg ataaacgaag
      181 atggaaattt tatgtatttt tattggtcta tgcaataaat aaattaaata ttcagaatac
      241 aattgatttt aacgaaaggg cttctatcaa tggttgtgtt gtatttatct gcccaggtag
      301 agtaaaatag atcattatct aaaaggtaat tgttcatact atataagtca tttgaaagta
      361 aaaagatact gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat
      421 caaaaatata gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg
      481 caagacagat gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag
      541 agaaagaatt ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa
      601 gtgaaaatgg tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta gcatccaaca
      661 attatcttgg atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa
      721 cttatggtgt aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc
      781 aattagaaga aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg
      841 gattcaactg caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat
      901 cggatgaatt aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca
      961 tccgaattaa acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa
     1021 gtaagaaata taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca
     1081 ttgcacgatt gccggaaatt attccgattg ttgaaaaata tggactgatc acctacgtcg
     1141 atgatgcgca cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat
     1201 taagcgacaa aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg
     1261 gttatgtagc tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc
     1321 tattttctac ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga
     1381 tgcaagaaca tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag
     1441 aattgaaaaa agtaggctat aacatcggta tgtctgaaac acctattact ccagtcattt
     1501 tgggagacga aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg
     1561 ctaaaccaat cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga
     1621 ctgcggaaca tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa
     1681 aagaaatgga aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat
     1741 gttcacgcat atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat
     1801 catgaaaaaa gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg
     1861 gctacgtcaa gaactaggca gtgaaaatgt cattgcgaca gatatacgca tgcctgaagt
     1921 cattgagaaa aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag
     1981 catagcagaa caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt
     2041 tgcagaagca aaaccacaat tagcctggaa tttgaacgtg actgggctgg tcaatgcatt
     2101 ggaagtcgct agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg
     2161 tccaaatact ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta
     2221 tggggtaacg aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt
     2281 agatactcgt ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg
     2341 tacgacagac tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag
     2401 tttcattgat caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat
     2461 tataaaactc atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc
     2521 ggcgatgtct tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt
     2581 tgaaatggat tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc
     2641 actagatgtt tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgt
     2701 gatgacaaag gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa
     2761 gaaccacatc caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa
     2821 gggatggaag aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa
     2881 cgtacgatgg gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg
     2941 gtatttttta aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt
     3001 tcttggtttc tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca
     3061 gctgctattc cagaaacagg agggatgatt aaatatatcg aacggattta cggaaataca
     3121 gcagcttttt tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc
     3181 ttgtcgatca ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt
     3241 gttcccgtag ctgttacagc tgctgtatcg atcctgttga tcaatttttt aggttctaaa
     3301 gctggagggg cttttcagtc gattacgctt gtctgcaagc ttattccttt gtttgtgatc
     3361 gtcattttcg gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca
     3421 ggagaaaatc tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca
     3481 tacgacggtt ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat
     3541 ttgccaaaag caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat
     3601 gcggtgttct taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt
     3661 gacgttgcaa aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg
     3721 atttctgttt atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt
     3781 atggcaaaag aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact
     3841 aaagttcctg tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc
     3901 ggtggttttg atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg
     3961 gtctttgttg gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa
     4021 gttccaatgt atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca
     4081 agcacgctta tcactcagac ctttttagct tcgatgggga tagtattgac acttgctggt
     4141 atcccgattt atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact
     4201 gcacttctct gccaaacaaa atattttatt taaatgagga gtacgtggaa gaaatgtcaa
     4261 aagtactcgt taattttgat cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta
     4321 aaagacagaa atcgggctca accgatttct gtcttttact ttgatttgcg taactgaact
     4381 attcggcgta cgccgcgata aatatttaat aaaaccaagc aaccagaaat gaaaatggga
     4441 agccaaatca gatagacagg gtagccaagc actttagaca aagcatgtgg agtcatttta
     4501 tccatttcaa gaagtgcttg ctgccaagtc tgaagattga agactagcat gaaacccgaa
     4561 ataatcagga gaataaatcc taaataatga attgtagttc tttttcttac ataagcgatc
     4621 gtagcataaa ggaacagtaa aaaaataggt acgccaaaaa caaagtacat attcagcatc
     4681 tcgacacccc ctttgtgttt atcttacttt actttgaaag aaatgagaaa taatagcctc
     4741 gttacttgct tgaaacagca aaggactgct aagataagag agatacaaag aatcaaggag
     4801 gagtcatatg gcaagttata tcgaaccgat tcgttttgcg ctgattgttt ttccattttt
     4861 agccttagcg atctcatctg ttttttttat ttatgaatat cgaaagtatg gaacatttct
     4921 agtaacaagg gcagttattt tgtattcttt cgttttttat ttattgtgtg cgtatttttt
     4981 ggtgatctta ccacttccgg caaaggagac ggttgcccaa atgacagggc caaagatgga
     5041 gctgcattta tttgcttcat ggcaacattt cagagacaac acggtattgg tcttgtctga
     5101 acctagtacc tacctgccgg caatgaagca gagtgtattt ttggaaccag cttttaatgt
     5161 tttattactc ataccatttg gcatttattt aagatattat ttccggcttt cttttatgaa
     5221 aacgatgatt gctagtttct gcctttctct attttttgaa ttgacgcagc taagtggact
     5281 ttattttatc tacccaagac cataccgctt atttgatgtg aatgatttag tgacaaatac
     5341 attgggtgga atcattggtt ttgtcgttac gccgttattt acatttatgc ttcctacaag
     5401 aagacgaatg gatgaacttt cttatgaaaa aggaaagact gtctcattga ctcgacgctt
     5461 ggtcgcttat ttgatcgact ggacattttt atctcttatt aatttggtag cggcgatttt
     5521 ctttaaaatt gcgttcgatt caggggcagt tacttctcat atctggtggc ttttattaga
     5581 agtaggggta tactttatga ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa
     5641 aattgtgcgt atccgtttgg tggaagaaga ccgcgaacga atttctttaa aagcattgat
     5701 gatccgctac gggtatctgt actttctttt ttatggttta agctggttgg ggcagttgct
     5761 tcagcaaatc atgaattcga caacgaatac ggtgttgcag atgagtgcag tattcatttc
     5821 tctcggtgtc ttgttactac aaggtatttt tattttaaat atcttgctat catttttgag
     5881 aaaaaaacgg attctgtttt atgaaaaagc tagtcacaca tatactgtta gcacgattgg
     5941 ccaaacaaag gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata
     6001 atgccaaagt cgtgataatt taaagagaag aggtgcacga atgaataatt ttgatatatt
     6061 ccgcttcaaa ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga
     6121 ggtccaggaa cgtttgattc ctttaattgc aaaaggaaaa aatatcattg ggcaatctca
     6181 aactggtaca ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga
     6241 aaaagaggaa gtacaagttg ttattacagc tccaagtagg gaactagctg cacagatcta
     6301 tgaagcagct acacaaatcg ctaaattttc tacaccggag atccgtgtct cgaattttgt
     6361 cggcggtaca gacaaacagc gtcaattaaa tcgtctgcat caccaacagc cacaggttgt
     6421 agtaggcacg cctggtagaa tattagacat gatgaatgaa caagcgctga agattcacac
     6481 cgcatttgca tttgttgtcg atgaagcgga tatgacattg gatatgggtt ttttaaatga
     6541 agtagaccaa attgcaagtc gattaccaga aaagctgcag tttttagttt tttctgccac
     6601 tattccagaa aaacttcgtc cattcttacg aaaatacatg gaaaatccaa tcattgaaga
     6661 aatcaaacca aaagcagtta tttctgaaga cattgaaaat tggctgattt caacaaaagg
     6721 taaagatgag aatcaaatga tttatcaact gctgacaatt ggtcatcctt atttagcaat
     6781 cgtatttgcc aatacaaaac agcatgtgga tgaaattgcc gattacttga aaaaccaagg
     6841 cttgaaagta gcaaagattc atggagacat caccccaaga gagagaaaac gagtgatgcg
     6901 acaagtacag aatctggagt atcaatatgt tgtagcgact gatttagctg ctagagggat
     6961 cgatatcgaa ggtgtctctc atgttattaa tgcagaaatt ccagaagatc tagacttctt
     7021 tatccatcgt gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca ttacgttata
     7081 ttcgccaaat gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct catttgaacc
     7141 aaaagaaatc aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg
     7201 agaaaaatca aaagatgtat tagatcccac gctgattggt cttgtgaaga agaaaaagaa
     7261 aaagatcaag cctggttata agaaaaaaat cgattgggca attgcagaaa aaaataaaaa
     7321 agaacgcaaa atcgaacgca gacagcaaag ccgtactgct cgtaaaaata agaaaaattc
     7381 acataaataa aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc
     7441 tagacttttt ttcattccga tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt
     7501 atgataccct tgtttttcat aaaaatctac agccgtcatt tcagctacta cataactagt
     7561 ctgacaacct tctgaaaccg cccgtttttc agcagcttca agcaactgac cgcctaaccc
     7621 ttttcctcgg tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt
     7681 cttttcataa cgcaaagtag ctactggtct tttttgatca agaaatagat aataggtttg
     7741 ttgcgggata tcttttccgt cgaattctag ttctgcagga atcccttgtt ctaatacaaa
     7801 aacttgatat ctcaaaaagc agctagctgc gcgtatccat tcatccgctc caactatttt
     7861 tttcatcata ttcctccttg tcatgaatag cagagatgta ttgattcctt tattcttatc
     7921 gtaccaaaaa tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga gatattgaca
     7981 gtgatcaata gaatgcgtat actaaataag tacgaaaagg atatgatttg ttttttaaga
     8041 ctcacagaaa attctttctt ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt
     8101 ttctattttg gtagaaatac cgacgcagct cagcgttatc gagtttaaga ggtaatgatt
     8161 acacatcatt gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg atggtgtttt
     8221 tttgtattta atttatagaa agcaggtaat gacatgaaac aattaagtag tgcacaagtt
     8281 cgacaaatgt ttcttgattt cttccaatct aaaggacata cgattgaacc tagtgcgtct
     8341 ttgatccccg tgaacgatcc aaccttattg tggatcaact ctggtgttgc aacattgaaa
     8401 aaatattttg atggttcagt tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct
     8461 attcgaacaa atgatatcga aaatgtcggt aaaacagcac ggcaccatac gatgtttgag
     8521 atgctgggta atttttcgat tggtgactat ttcaaaaatg aagccatcca ctgggcatgg
     8581 gaatttttga ctgatccaaa atggatggca ttcgatccag aaaaacttta tgtaacagtt
     8641 tatccaaaag atacagaagc aaaacgcatc tggcatgaag aagttggcct gccactagat
     8701 cacattattg atgtagaaga caacttctgg gatatcggtg caggcccatg tggtccggat
     8761 acggaaattt tctatgaccg tggagaagct tataacgatt tagcagaaga cgatcctgaa
     8821 aactatccag gaggagaaaa tgaacgttat ttagaaatct ggaacttagt attttcagaa
     8881 ttcaaccata agcctgataa cacatttgaa ccacttcctc ataaaaacat tgatacgggg
     8941 atgggattgg aacgcatggt ctcaatcgtt caagatgcgt caacgaactt tgagacagac
     9001 ttgtttatgc ctattatcca cgccgtagaa aagctaagca aacaagtaaa atatggtgaa
     9061 aacccagtaa cagatatttc ttacaaagta atcgcagatc atattcgcgc cctttcattt
     9121 gctattggcg acggtgcatt gccatcgaat gaagggagag ggtatgtctt acgccgtttg
     9181 cttcgtcgtg ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt
     9241 ttagtccctg ttgttgggga aatcatggta agttattatc ctgaagtact tcaacaaaaa
     9301 gaatttattg aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa
     9361 ggtttggaaa tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta
     9421 gcaggaaaag acatcttcaa actatacgac acatatggct tcccggtaga attaacggaa
     9481 gaggtggcag aagatgaagg actgaaagtt gatcatgctg gttttgaaaa agaaatggat
     9541 gcacaacgcg aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg ggtacaatct
     9601 gctttattga cagatatcaa agtagaaagt aaattcattg gatacacaga acaagaatca
     9661 gatagtcaat tattggtcat cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt
     9721 gaagctcaaa tgatctttga tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg
     9781 gatcaaggat ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac agatgttcaa
     9841 aaagcgccta atggtcaatt tatgcacacc gtagaagtaa aagcaccaat gagtgaagga
     9901 caaacctatc atctattgat cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca
     9961 gcaacccatc tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct
    10021 ggttctttag tcgctcctgg acatttacga ttcgacttta cccactttgg tcaaatcact
    10081 tcagatgaat tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga agctatccca
    10141 gttgaaacaa ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat ggctttgttt
    10201 ggtgaaaaat acggacatga cgttcgagta gtaaatattg gcggctggtc aatcgaactg
    10261 tgcggaggca cacatgtagc aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa
    10321 tcaggaattg gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa
    10381 ctacttgaag aagaagaaaa acaactaaaa caagtagcaa caattgttaa atcacctcaa
    10441 ttgaaagaag ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga gctacaaaag
    10501 gaaaatgaac aattagctag caagttagcg aatcaacaag ctggtgatgt attcaaagat
    10561 ccagaaacga tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa agatatgaat
    10621 cagctgcgtc aattagctga ccaatggaaa caaaaaaata tttcagatat tcttgtttta
    10681 gcaaacgaac aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa
    10741 gctggactaa aagctggcga cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt
    10801 ggtggacgtc cagacatggc acaagctggc gggaaaaatc ctgctggtat tccagatgca
    10861 cttaaagctg cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg acagaaaaac
    10921 aaaagttcaa acattgaacg aaacatgatt atgggggcga agacaaacaa gtcttgccct
    10981 catacttttt tcaattggat ggcgcatcta atcaaatggt cggattcgga tggtctttag
    11041 ttgacgaaag tgacagaaat ttttacgatt agtataaata aaaaggggtg aaaaaatgaa
    11101 tagtagaaat aagacctttc gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt
    11161 acaagcgatg gttccttggc tggggtttat tccattagga ttcattagtt taacaattat
    11221 ccatatcaca gtaattattg ctgcagtagt attaggccca aaagatggca tggtgattgg
    11281 cttgttttgg ggaatcgcta caattgttcg tgcctatgcc atgccaacaa cgccatttga
    11341 tacattagtg ttcacgaatc cgattatctc ggtagttccc agagtacttg ttggcttagt
    11401 agcaggcttg gttttccact ggatttatca aaaaaataaa tcaataacga tcagctcagc
    11461 ctttgcaggg gtactgggtt cattggtcaa tacagtctta gtgttaggat tcatgggatt
    11521 attttataca ggtgcaacgg cagatgccta tggtgtagac tctgctcttt tatttaaaac
    11581 cttagcaggg gttgcagcaa tcaatgggat accagaagca ataggtgcgg gaatcataac
    11641 accattacta gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat aatgaccatc
    11701 ttttaatagt aataggacgc acgatagata gaaaaaggaa gagaggagag gaaaacaatc
    11761 gtcctctcca tctttttacc tgagcaacaa gcaaactaga aggggtaaaa aacttggcta
    11821 agaaacaaga agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag
    11881 cgggtatctt aggattggtt acgaatctga tcttgtttgt ctctaagcta atgatcgggc
    11941 ttttttcagg aagtgtctcc attatggctg atgcagtgaa cagtctatca gatactgctt
    12001 catccatatt gactttgatc ggatttaaaa ttgctgcaaa gccagcagat caggaacatc
    12061 cttatggaca cgaacgcttc gaatatatca gtgggttatt tgtttcgatc atcattactt
    12121 atgtaggatt tcaattttta gagtcttctg ctgaaaaaat catccatcca gaaaatgtct
    12181 ctttaacacc catcgtcttt tttgtcctaa tcatttccat ctttttgaaa tttgcacaag
    12241 gcaaaatgta tcaggttatt gcgcaatcca ttgattctga aacattgaga gctacagcaa
    12301 cagatagtta taatgatgtc tttactacac tggctgtatt gctttcagca gcaatcgaat
    12361 gggtaactgg atggcgtatt gatggatata taggttttgc tttagcatta tatatccttt
    12421 atagtggatt gacgatgctt cgaaattttg tatacgaact attaggaagt cgcccaacgc
    12481 cagcagagat tgaagaaatg gaagagcatt taaatgcttg cgatgatata cttggctatc
    12541 atgatttatt ggtgcataac tatggaccca ataaacgctt tgcatcagtc cacatcgaag
    12601 tagatgaatc aatgaatttg aatgtggctc ataagattat tgatctgata gaaaaagact
    12661 ttaaaaagtc tttggatgtg gaacttgtct gtcacttgga tccaatgcct gttgataacg
    12721 aaaaatacta taatatcttg cggcagttaa agcaaattct tcttgaaaca ggtacagggc
    12781 ttaatattca tgattttaga gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg
    12841 tccctgagaa attccagttt gttgatacag aactagaaag aatcttacgt agtcaaatag
    12901 aagaaaaaat cggagattac caattggaaa ttacgtttga tcacaattat cttttatatt
    12961 aaatgagaaa ctataaaaag actccttaaa gtttttaatt taaagagtct ttttttcttt
    13021 taattgttta tggagagcct gacgtgcaag attatctgct cctttatttt tgttttcggg
    13081 caaccattgc aggatcaata aagaaaattc ggatagaagt tcttggattc tttgaaggta
    13141 gggaaggaag agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct
    13201 gtctgaaaaa cagaaaatcg tttcattctg taagtcgttt ttctttagaa attctaaagt
    13261 ttttaaaaag acttcgaact ctgcttgatg gtttgtccct atagaaagcg ggatagatag
    13321 ctgttcttgt ttaccttgat agacaatcaa gattccacct ccactaggac cagggttgcc
    13381 ttttgtagag gcatcaatgt atacttttaa cataaaaaga gattctcctt tttgttcaaa
    13441 ctaatgttat ggtaagatta tatcattact gaatcggtga gaggttagag aatgaaaaaa
    13501 aatattcgat ggcagcctga actggcacat acgatcatct attggtcatt gaccttgatc
    13561 attctgtttt ttagtctgat tctttcttta gaaaacacag gtccttattg gaaaagcaat
    13621 tttgttttag gtatttttct tgtttttcta tttctaggtt tgcggcgttc ttttatttta
    13681 aaatcaaact ctgtgattat acgttatgct gctttttgga aagaccgaga aatatctgtc
    13741 ggacagattc gatctttgac gccaactaat actggggtgc aaatgatgtt gcaagatgag
    13801 aaagccccat ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt
    13861 gaaaaaaacg gacccaaatc agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc
    13921 taattttaaa aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa agagtgagac
    13981 gtacggttca tcccgtttag cgtctcactc tatttgttta tctattcatg catactagta
    14041 tcggtagtct gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg attcccttcg
    14101 atgatctcaa attcgaccac ttgattttct tccaaagttt tgaatccctc ttcttcaatc
    14161 gcagtaaaat gtacaaaaat ttcttctgtg tcatcataac tgataaagcc gtatcctttt
    14221 ttgttgtcaa accattttac aattcctgtg gtcaaagcac tcacgacctt tctaaagtct
    14281 gatcatagcc ttcattataa agagtattta taagtacgtc aaataaatta aaaacctgta
    14341 attttatgtt ttttttctga ataaaaaaag gtataattgg catatcttaa caaagaagat
    14401 attttgttga ataaagttcg tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc
    14461 acaagaaaca gagctagaac cgattgttga agtggctagt aaattagatc ttaaggaaga
    14521 cgatttggaa ctgtatggga aatataaggc gaaaatcaat ttttctggaa tcaatcgatc
    14581 aaaggataac gctgaggggc atttgatctt agttacttcc atcaatccaa caccagctgg
    14641 cgaaggaaag tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa
    14701 atctgttatt gcattgcgag aaccctcact tggtcctgtg atgggaatca aaggtggtgc
    14761 tgcaggcgga ggatatgcac aggtattgcc tatggaagat atcaacttgc attttactgg
    14821 tgacatgcat gctatcacaa cagctaataa tgcattatct gctcttttag ataatcatat
    14881 tcatcaagga aatgaattga acatcgatgc ccgccgagtg atttggaaac gggtagtcga
    14941 tctcaatgat cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt
    15001 tccaagagaa gatgggtttg atatcacagt tgccagcgaa atcatggcta tcttgtgtct
    15061 tgcggcagac cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg gctatacatt
    15121 tgaccgtcag cctgtgactg ctggagactt gaaagcagaa ggcgctctgg cattactgct
    15181 gaaagatgca atcaagccga atcttgtaca aacaatttac ggaacaccag cttttgtcca
    15241 cgggggaccg tttgccaata tcgctcatgg ctgcaatagt attttagcta ccaagacagc
    15301 attgagatta gcagattacg ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga
    15361 gaaattttta gatatcaaag tacctaactt gaaaaaagcg ccggatgctg tggtgattgt
    15421 cgccactgtc cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga
    15481 gaatcttgat gctttgaaag taggttttgc aaatttaaag cgccatatac gtaatatgga
    15541 acaatatcag ctacctgtga tcgtcgcaat caatgaattc gtaacagata cagacaatga
    15601 actcacactg ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt
    15661 atgggcagat ggagcagaag gcggcgttga tttagcaaaa gctgtagtac gattgattga
    15721 tagaaaagaa gctaattaca agccattata ccgcctagaa gaaacgatac aagaaaagac
    15781 agaaacaatc gtcaagaaaa tttacggagg aaatggtgtt gttttcagca aaaaagcgca
    15841 aaaacaaata gaagagttta cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa
    15901 aacacagtat tcattttctg atgatccttc actgctaggt gcaccggaag atttcaccat
    15961 cacgatccgc gaaatcatcc caaaattagg ggcaggattt ttagtagcct tgactggaga
    16021 tgttatgacg atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa
    16081 tgacggagaa gtgttgggat tgttttaagc ttgattataa tctaccatcg aaggagctgt
    16141 attttgacta cagcttcttt ttttagacga tagggaatta attttgttta cactaggatc
    16201 attctttttc tttattgtat tttgagctat actaaagcat aataggaggg tatgatgaaa
    16261 acgacattta taagaaaaac cggtttttac ggtatgggta gctcgattca gctaagaatc
    16321 aacaatgaaa aacgcagtgt tctcagtcat gaacaaaaga taacgcttga tctggaagtg
    16381 ccctttacga tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa
    16441 gcaaagcctg cttatgtgat cacaatgaat tacttgattc ttcaaatcta tccgttttta
    16501 tttctagcaa ctgggttggg ggccgttttc ctacaaaatt tatttttcag tttttttgca
    16561 gttattgtgt tgattgcgtt ctttttctat ataaaaaacc gggcatattt gatcaaggag
    16621 gaaaattatg gcaaactctg atcttttttt aatcagtttc aatcgaatcg aaaaatggat
    16681 gagagacgag atgggaaatg cacgaaatat gggatttacc gaacttgtca gacgcctagc
    16741 tcaaagaaaa caattaatga tcagaaaata cgaagatgat cttttacagc ttgctcagtt
    16801 aaggaatgcg atcgtccatg atcggatcgc agtcgatttt gtcattgcag agccaaatga
    16861 ttggacagtc aagcgaatcc agctgatcga acaagaactg attcagcctg aaaccgtgct
    16921 gcctcgattt gcaaaacgtg taacaggttt tgaacaagat cttccgttac ttgaactgtt
    16981 aaagatagta gcagaaaaaa ggtattcaca atttccgtta tacaataaag gaaaatttgt
    17041 agcattgatt actttacgta atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc
    17101 ggttgattta acaaataaac gagcgttgga tttgatcatc caaaacggaa agtacacaaa
    17161 ttatcatttt gttccagcta aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca
    17221 agggactttg gaagccgtac ttatcactaa agatgggaat cccgatggta atctgctcgg
    17281 gatcgtacga cctcgtgata tttataaaca aatagaaaag gattgatttt ttgttaatta
    17341 tttattggat attcagcgct ttgctagtag gagtggatca gttggtcaaa tggtggatca
    17401 ctgataatct agcgcttgga gagacgaaaa atcttatacc aggtattttg tcactgaatc
    17461 atattcgaaa cactggagcc gcatggagta tgctagaagg gaagatgtgg ttctttacta
    17521 tagtgacact cattgcagtc gttgtgattc tcacattgat gattaaaaat cgtgaaaaag
    17581 gtaatcgctg gttcatgatt ggattgagtt tgattctagc tggtgcgatt ggcaatttta
    17641 tcgaccgtat tcgtcttggc tatgtagtag atatgttcca gacagatttt atgagctttc
    17701 cgatttttaa cgtagcagat gtgacacttg tatgtggagt gatttgtatg ttaatttata
    17761 ttattttaga tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga cgattcacca
    17821 tgaatctggt aggatcgata aagtattgac taatttattc accgattaca gtcgttcgca
    17881 aattcagctg tggctgaaaa atggagcggt ttctgtcaat ggacagccag tcaaagcaaa
    17941 ttacaaagta aaacaaggag acgagattac tgtcttgatc cccgaaccag aaacattaga
    18001 tgtggtccca gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg tagcggtgat
    18061 aaacaaacct caaggaatgg tcgttcatcc ttcagcaggt catacgcatg gcacaatggt
    18121 caatgcgctg atgtaccata tcaaggatct ttctacgatc aacggcgtta ttcgtccagg
    18181 tattgttcac cgaatcgaca aagatacttc cggactgcta atggttgcca aaaatgacca
    18241 ggcacacgaa tcattagctc agcaattaaa agataagacc tctttgcgca aatatgttgc
    18301 tttagtccat ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc
    18361 gaaggaaaac cggaaaatgc aggcgataag agaagatggg aagtcggctg tcacacattt
    18421 tactgtattg gaacgtttcg aaggatttac tctggttgaa ttaaaactag aaaccggaag
    18481 aacacaccag atccgtgtgc atatgaagta tatcggttat ccgttagctg gagatccagt
    18541 atacggaccg aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg
    18601 ttttacgcac ccgactacaa acgaaaagat gatctttgaa gcacctcttc cagaagtttt
    18661 tgaaaaaact ttagaaaaat taagaaaaaa cattgcattt taatttagat aacggtatag
    18721 taattaacaa cgaaagcata aaacttaata gaattccttt aagtttagtc ccgtgaggct
    18781 aagaagggtg ggagcaagga tacgtatggt gcgccataag tagattgctc attgacatag
    18841 tatagctatc agtgtatcag tatacgctca gtctaaacat tcctcctgtc ctcttagggc
    18901 aggagttttt ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag aagtagtaga
    18961 tcaggtaacc atgaagcgag cattgactcg aattacttat gagattatcg agagaaacca
    19021 tagtatccaa gatatcgtat tggtaggtat caaaacacga ggaatctaca ttgcatcaag
    19081 aattgccgaa cgcctgaagc agctagaaga tatcgatatt cctgtaggag aactggatat
    19141 cactttatac cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc attcttctga
    19201 catcccagtt tccttagaag gaaaagaagt tattttgatt gatgatgttt tgtatacagg
    19261 acgaacgatt cgagcagcaa tggacgcagt aatggatttt ggcagaccaa gaaaaatttc
    19321 attagcagtc ctggtcgatc gtgggcatcg agaattgcca atccgtgcag attatgttgg
    19381 gaagaacatt ccaacatctc gggcggaaga gattttagta gagatgcaag agcttgatgg
    19441 acaagatcgc atcatgattt tgaaagagga agattagagg agagagaaac atgtcggaga
    19501 aaaaaggatt tcataatgat gacgtggtgt tagatatcca cgatcgccca accccaggac
    19561 attgggtagg attaagttta caacacttgt tcacgatgtt cggagcaacc gtgttagtcc
    19621 ccatcttagt aggtatcgat ccaggtattg cgctagtcag ttcaggacta ggtacattgg
    19681 tgtacttatt tactactaaa ggaaagatac ctgcgtactt aggaagcagc ttcgctttca
    19741 ttgcagcgat gcagcttttg atgaaaacag atggttatcc agcgattgca caaggagcag
    19801 tcacaactgg attagtctat ttgattgttg cgctgatcat caaaaagatc ggttcagcat
    19861 ggctggataa aatcttgcct ccaatcgtcg tcggtccagt catcatggtt atcgggttgg
    19921 gacttgcggc aaatgcagca acaaatgcta tgtataatca gggcacgtat gatttcaagt
    19981 atattgctgt agcgctgatc acattaggat taacaatttt cttcaacatg tggctaagag
    20041 gttttctcgg attgattccg attttactag gtattattag cggatacatc gtagccttgc
    20101 tgtttggaat cgtggatact aagccaatcg tagacgctgc ctggtttgct gttcctaact
    20161 ttgagattcc ctttgtacaa tatgaaccaa agctatatct tggagcaatc acaacaatgg
    20221 cacccatcgc ttttgtgacg atgacagagc atatcggcca tcttatggta ttgaataaac
    20281 taactaaacg taatttcttt gaagaaccgg gtcttcataa gacgttgaca ggtgatggat
    20341 tagcacaaat cgtggcagcc tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca
    20401 ttggtgttct ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat
    20461 ttgcagtcat tttaggattc gtaggaaaat taagcgcttt gattttaagt atccctggac
    20521 cagtcatctc aggaatcagt tttatcttat ttggagtaat tgccgcaagt ggaatgaaga
    20581 ttttaatcga gaacaaaatc gattttgata aaaagaaaaa tctgttgatt gcttcagtta
    20641 tcttagtagt aggaattggc ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa
    20701 tggcattagc aactgtactt ggcatcattt taaatcttat cttaccagaa acttcacgaa
    20761 gcgaagaaaa ctaaggagga aacattatga tcatcaaatc agaacgtatc agcttaaagc
    20821 atttattgac cgttgaggca cttactgatc aagaagttat gggcttgatc cgcagagggc
    20881 aggaattcaa gcaaggtgct aattggactc cgcaaaagcg ccagtacttt gctacaaacc
    20941 tctttttcga aaatagtact cgcacacata aaagcttcga tgtagcggag aaaaaactag
    21001 ggctagaagt gattgaattt gaagcaagca caagctctgt ccaaaaaggg gaaacactat
    21061 acgatacagt actgacgatg tcagcattag gcgtagacgt agcggttatc cgtcatggtg
    21121 atgagaacta ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg
    21181 gaggagacgg aagcggacag catccaaccc aatgcttgct tgatttgatg accatttatg
    21241 aagaatttgg tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc actcactctc
    21301 gcgtagctaa atccaatatg cagatgttaa aacgtctagg cgcacaagta ttcttctcag
    21361 gtcctagaga atggtacgac gatgaattcg aagtgtacgg acattatatg ccattggatg
    21421 aactactaga tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg
    21481 gaaaagaaag tttctcaaaa gaaggctacc atcaggaaca tgggttaaca gttgagcgtg
    21541 cgaagaagtt gcaaaaacat gcaatcatta tgcacccagc accagttaac cgtgatgtag
    21601 aattagctga ttcattagtg gaaggattac agtcgcggat cgttcagcaa atgagcaatg
    21661 gagtattcgt cagaatggca atcttagaag cagtgctaaa tggaaaagcg taattatagg
    21721 aggatgaaaa aatgaaaaca ctcattaaaa acggtcagat caatacacga aaaaatatga
    21781 cgacaccagc agaaatttgg atagaagatg ggcgaatcaa agcaatcggt acaggattct
    21841 cagaagccga atttgatgaa gtgtttgatg caaagggaca attgatcaca cctggtttag
    21901 tggacgtcca tgttcatttg agagagccag gatttacata caaggagacg atcgaagctg
    21961 ggactcgctc agctgctcgt ggcggtttca ctactgtatg cgcgatgccg aatttaaatc
    22021 ctgtacctga tacggcagaa aagctgagac aagtatatga catcattaga aaagatgcag
    22081 ttgtgaaggt attgcagtat gcgccaatca cagaaaatct gcgaagtgag aaattagttg
    22141 atcaagaagc actgattgaa gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc
    22201 aaacagctgg gacgatgtat ctggctatga aagaagctgc aaaaaacaat aaagccctag
    22261 tagcccatac agaagatgaa tctttattat ttggcggcgt tatgcacgct ggcaagaagg
    22321 cagaagaact tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa attgctagag
    22381 atctgttgct agctgaagca actggtgttc attaccatgt ttgccatgtt tctactaaag
    22441 aaagtgtccg tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca gcagaagttt
    22501 caccccatca tcttattttg atcgacgaag atattcccga agatttcggc ttttggaaaa
    22561 tgaacccgcc gctaagagga cgggaggaca gagaagcttt gatcgaaggc ttgcttgacg
    22621 gtacgatcga ttgcatcgct acagaccatg ccccccatgg attagaagaa aaaagccaaa
    22681 gtttcatgaa atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa ctgatctata
    22741 ctcattttgt agaaactggt cgattcacgt tggaacaagt gatcaattgg ttggcagtta
    22801 aaccggctga gatttttggt ttgaatgcag gaacgctaac ggttggtgcc cccgctgatg
    22861 tagcagtttt tgatattaca caaacttgca cgatcgataa agaagacttt ttatcaaaag
    22921 gggagaatac accttttatc ggctggaaag taaaaggcga aacacaaatg acttttgtca
    22981 acggaaaact tgtgtggcag aagggagaat aaaagtatgg aacgtttgct tatattagaa
    23041 gatggaacag ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt cggagaaatc
    23101 gtttttacaa caagtatgac tggttatcaa gaaactatca ctgatcaaag ttttaacggt
    23161 caaatcatta catttaccta tcctatggtg ggtaattatg gggtaaatag agatgattat
    23221 gaatcgattt ctccaacatg caaaggagta gtagtaaaag aacatgcacg tgtagcttca
    23281 aactggcgaa accaaatgac gctagattca tttttgaaac aaaaaggaat tcctggaata
    23341 tcaggtatcg atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac gatgaaagct
    23401 agtatagttg atgctggaga ttccttcgaa catgcatttg atcagctgaa agcaaccgtt
    23461 ctagcaacca atcaagtgca gcaagtctca actaacaaac cttatccaag tccaggaaca
    23521 ggtcggaaag tcattgtcat tgacttcggc ttgaaacata gtattttacg agaattgagt
    23581 aaaagaaact gtaacctcac agtacttccc tataacacag atgccgagac gattttagcg
    23641 ctatcaccag acggagtcat gctgacgaat ggacctggtg atccaaaaga tgtgccagaa
    23701 gcaatcgaaa tgatcaaaaa aatccaagga aaagtaccga tttttggtat ttgtctcggt
    23761 caccagttat ttgctttagc caacggtgca gatacctata aaatgaaatt cggacatcga
    23821 ggattaaatc acccggtacg agaagtagcg accggaagaa ttgattttac ttctcaaaat
    23881 catggctatg cagtcgatga agccacgatt gatccagaaa aactcatcgt aactcatgta
    23941 gaagtaaatg acggaacagt agaaggagtc aaacacagag attatcccgc tttcagtgtg
    24001 cagtttcatc cagatgctgc ccctggtccg catgatgcac tacatttatt tgatgaattt
    24061 atggaaatga tggacgcagg aaaggagcag taaacagatg ccgaaacgta cagatatcaa
    24121 gaaaattatg gtgattggat ctggtccgat catcattggt caagccgcag aatttgacta
    24181 cgcaggaaca caagcctgcc ttgccttaaa agaagaagga tatgaagtgg ttttagtaaa
    24241 ttctaatcct gcgacgatca tgacggataa agaagtggca gatcaagtat atatcgaacc
    24301 gatcacactt gaatttgtat caaggattct tcgaaaagag cgtccagatg ctctccttcc
    24361 aactttaggc ggacagacag gcctcaatat ggcaatggag ctggcagcat caggtatttt
    24421 agaagaattg aacatagaac ttctaggaac aaagctttct gcgatcgatc aagcagaaga
    24481 tcgtgatcta ttcaaacagt tgatgaaaga attgaatcag ccaatccccg agtctgaaat
    24541 cgtcaataca gtagaagaag ctgtagattt tgcaaataaa atcggctatc ctgtcatcgt
    24601 tcgtccagcc tttactttag gcggaactgg tggaggtatg tgtgaaaatg aagaagaatt
    24661 acgtatcgta tcagaaaatg gattaagtct ttcccccgct acccaatgtt tgattgaacg
    24721 aagtattgca ggttttaaag aaattgaata tgaagtgatg agagattcgg ctgataatgc
    24781 aatcgttgtc tgcaatatgg aaaattttga cccggtgggt atccatacag gtgattctat
    24841 cgtatttgcc cctagtcaaa cattgtctga ccatgaatat caaatgcttc gtgatgcttc
    24901 tctttcgatt attcgagcac ttaagatcga gggaggctgt aatgtccaac tcgctcttga
    24961 tccacatagt tttaattatt atgtgattga agtcaatccg cgtgtttctc gttcatctgc
    25021 gttagcaagt aaggcgactg gctatccgat cgctaagcta gctgctaaaa tagcgcttgg
    25081 attaacatta gacgaaatga aaaatcctgt tacaggaacg acttttgcag aatttgaacc
    25141 tgcattagat tatgttgtag ctaaaattcc tcgctggccg tttgataagt ttgaaaacgg
    25201 agaacggctt ttaggcacgc agatgaaagc aactggtgaa gtgatggcta tcggtcggaa
    25261 tatcgaagag tctttattga aagctgtgcg ttctttagaa atcggtacac accatatcga
    25321 acttcctgag ttggcagaca tcacggacga tgacctaatc caaaaagtgg ttcgagcaca
    25381 agatgatcgc ctgttttatg tagctgaagc tattagaaga gggtacacca tagaagaatt
    25441 agcggatttg acaaaaatcg atttattctt tttagataaa ctgttgcata tcgtagaaat
    25501 cgaacagcag ttgaaacagc agccgttgga tgacaagata ttgaaaacag cgaaacaaaa
    25561 cggttttaca gacaaaaaag tggcggagct gtgggaaata aacgaagaag ccgttcgcca
    25621 gatgagaaaa gaaaatgatt tgattcctgt atataagatg gttgacactt gtgcagcgga
    25681 attcgaatca aaaacacctt atttttatag ttcctatgaa tatgaaaatg aaagttcacg
    25741 atcagaaaag ccctcaattc tagtactggg atctggtcct atccgtatcg gacaaggggt
    25801 tgaatttgac tatgcgactg tgcattctgt caaagcaatc cagcaggcag gatacgaagc
    25861 aatcatcatg aacagcaatc ctgaaacagt ttcgactgac ttttctattt cggataaatt
    25921 atattttgag ccattgacct tcgaagatgt gatgaatgtg atcgagctag aacagccgat
    25981 aggagtaatc gttcaatttg gtggtcagac agcaatcaac ttagccgaac ctttgactgc
    26041 tgctggtgta aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg aaaatcgtga
    26101 tttattcgag caagcgctga aagagttgga tattcctcaa cctttagggg atactgctac
    26161 aagtaaagaa gaagccgtgg ttatcgcttc gagaattggt tatccagttc ttgttcgtcc
    26221 aagctatgta ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag atttagaaga
    26281 ttacatggaa catgctgtga aagcatctcc agaacatcca gtactagtgg accgttactt
    26341 gcttggtggt gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac tcatcccagg
    26401 gatcatggag catattgagc gtgctggtgt ccattcaggg gattcaatgg ctgtgtatcc
    26461 acctcaaaca ttatctgaat cgatcaagaa aacgatcgaa gattacacga tccgcttagc
    26521 attaggtttg aattgtgtcg ggatgatgaa tatccagttt gtcatccatg aaaatgaggt
    26581 ttatgtgatc gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa gtaaggtgac
    26641 aggaattcca atggcacaaa ttgcgacgaa agctattttg ggagagaaac taaagacact
    26701 tggttatgaa aacggacttt atccagaaac aaaagaagtt catgttaaag cacctgtctt
    26761 ttcttttacg aaattgcaga aagttgatac ttacttaggg cctgaaatga aatcgactgg
    26821 agaagtaatg ggatctgaca gacaattaga caaagctttg tacaaggcat tcgaagcttc
    26881 tggattacac ttgcctgaat atggagccgt attatttacg attgcggacg aaacaaaaga
    26941 agaagcacta gaattagcta aacgttttac agaaattggc tatagtctga taggaacgaa
    27001 acatacagct gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag cgaaaatttc
    27061 tgaggaagca gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa cacaagtggt
    27121 tgtcaatacg attgataaag atcggggaaa tgcttctaaa gacggtttca tcatccgaag
    27181 agaagcagtt gaacatggta ttccattgtt tacttcttta gacacagcag atgcgatcgt
    27241 tcgagtgatg gaatcccgtg ctttttcaac taaccctatt taacggcgag cacatgttaa
    27301 gacaaaaaga cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat gatggaaatc
    27361 gttcagcaaa aacagctggc accaaagatt tttgaaatga cattgaaagg tgatctagta
    27421 aaagaaatgc agcaaccagg acagttcttg catattctcg tgccgcgtgc agacttgcta
    27481 ttacgccgac cgattagctt gaatcatatc gaccacgaaa caaatacttg ccgtatcatt
    27541 tatcgggtgg aaggtgaggg gactagagtc ttctctactc tttctgccgg agaccattta
    27601 gatgtcatgg gtcctttagg caatggtttt gatttgtcca atcttcatac aggagacgaa
    27661 gtatttataa tcggtggagg aatcggtgtc cctcctctat atgagttatc aaaacagtta
    27721 gtcgaaaaag gaattaagcc aacgcatttt ttaggttttg ccacacatga agtcgtttat
    27781 tatgaaaaag aatttaatcg tctaggagaa acaagaatcg ctactgacga tggaacatac
    27841 ggtatgcacg gaaatgtcgg taatttattg ttaggaacga agacggaacc tacagctgtt
    27901 tttgcatgtg gttcaaacgg gttattgaag acagtggaac agctgttttc ttcgcatcaa
    27961 aatgtccagt tatcattaga atcccgcatg gcatgtggga taggggcttg ctatgcttgc
    28021 gtttgtcata cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg tgacgaagga
    28081 ccgattttta aaataggaga ggtaattata tgaatgagaa tcctttagcg gtcaaacttc
    28141 ctggcctaga attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt tttggcaaag
    28201 aatacggaaa atactatgat ttgaatcagc ttggatcgat catggtcaag gcaacgactc
    28261 caaatgcccg ctttggcaat cccacgccaa gagtggcgga aacaccatct ggcatgttga
    28321 atgcaattgg tttgcaaaat cctggtctag acgttgttat gcataaactt cttccagaat
    28381 tagaggagta caatgagtta cccatcatcg caaatgttgc tggtgcttgt gaagaagact
    28441 atgtagaagt ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc gagctgaaca
    28501 tttcttgccc aaatgtcaaa catggcggaa ttgcttttgg aacagattct gatgttgctt
    28561 ttcaattaac acaagcagta aagaaagttt cttctgttcc tgtctatgtc aaattgtccc
    28621 caaatgtaac agacatcgtt ccaatcgctc aggcaatcga agctggaggg gcagatgggt
    28681 ttacgatgat caatactctt cttggtatgc ggattgacct taagacaaga aaaccgattt
    28741 tagccaatca aacaggtgga ctctccggac cagcaatcaa gcctgtcgct attcgtttga
    28801 ttcaccaagt agcaagtata tccagccttc caatcattgg gatgggcggc gtgcagacag
    28861 tggatgatgt tcttgagatg tttatggctg gagcaagtgc tgtagctata gggaccgcga
    28921 atttcaccga tccgtatatc tgtcctaaac taattaaaga gctccctgtg cgaatgagcg
    28981 aactaggcat cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa cgtgaacgat
    29041 gaatcaacta gaccaacgac caattattgc attagatttt tcaactaggc aagaggtaga
    29101 agactttctt cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg gaatggaatt
    29161 gttctaccaa gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc ataatgtatt
    29221 tttagatctg aaacttcacg atattccgaa tacggtcgaa aaagcaatgc ggggattggc
    29281 aaagcttggt gttgatctca cctgtgttca tgccgctggc ggtattcgta tgatggaggc
    29341 ggcaatgcga ggattagaag aggggactcc agaaggtgga aaacgtccgc tacttctagc
    29401 tatcactcag cttacttcta caagtgaaga agaaatgcat gccgatcaac taatcgaagt
    29461 ttctttagaa aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag gattagatgg
    29521 tgtggtttct tctgcttggg aagtagaagc gatcaaagaa atggcaggag acgaatttgt
    29581 ttgcttaact ccgggtataa gaccagaagg aacagtcgca ggagatcaga caagagtagt
    29641 cacaccttct caggcaaaaa agatcggctc aacttttatc gttataggtc gaccaatcac
    29701 tcagtcaact aatccttata aagcatatca aacgattcaa acagaatgga gctaacctaa
    29761 aatgaatgtt gaacaaagta tcgcaaaaga cttattagaa attgaagcag tatttttaaa
    29821 tccaagtaat ccttttacat gggcaagtgg aatcaaaagc ccaatttact gtgataaccg
    29881 aatcacgatg agctatccaa aagtccgaaa agagatcgca aaaggattag caagcaaaat
    29941 caaagaagct taccctgaag tacaagtgat tgctggaact gcaacagcag gaatccctca
    30001 tgccgcatgg gtagcggaaa tactggattt gccaatggtc tatattcgaa gtaaggcgaa
    30061 agatcatggg aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga agatggttgt
    30121 gatcgaggat ttgatttcta caggcggaag tgtcttggaa gcagctgaag cagcaaaaag
    30181 agaaggtgcg gatatccttg gtgttgcggc tatctttacg tatgagttgc caaaaggaaa
    30241 agcaaatttc gaaaaagccg aaatccctct tatgacgttg accaattatt ctgtattgat
    30301 tgaagcagct ttagaagatc gttatatcga tgagcaagaa ttaactctgc taaaagaatg
    30361 gaaaaaagat cctgaaaatt ggcagattta agcgcgcttg atgaagatag agaaatccga
    30421 tttgtgtctc cagaatcatc aattggaata gcaaaaatcc aaaaactgat tgcgagaagc
    30481 atcttttcat caggtatgaa aagaaacttc tatcgtgatc agtttttgat ttttttaaaa
    30541 ttcatttttc taagactggt tagcagaaag aagagaataa tctcttctct tttttctatt
    30601 atatatagat tttctctatt ttttggtaag ggagctgttt caaaaatggt aaagagcgga
    30661 aatgttgcga atatcagtta tttaatgata tgatattcgg aaaaagaaat ctcaaaggga
    30721 ggtaactttt atggggttga atgaatttat tttaggattg aacaatttag agacgatcac
    30781 aagggcacct ggatttttta aatatacaga gcatacagtt gcagcacatt cgtatcgtgt
    30841 agcatctatt gcgcaagttc taggagacat tgaagaagcg aatggacaac cgatcgattg
    30901 gaaactgctc tatgaaaaat cattgaatca tgactacaca gagcgtttta ttggtgatat
    30961 caaaacgccg gtgaaatacg caagcaaaga gttaagaggg atgctgcaaa cagttgaaga
    31021 aaagatgacg gacgaattca tcaaccaaga gattccagct gagttccaag aagtttatcg
    31081 ccgtcggttg ttcgaaggaa aagatgaaac cgtagaagga gaactattag ccatttcaga
    31141 taaggttgac cttctttatg aatcttttga tgaaatcatc aaaaataacc cggaaaaagt
    31201 ttataaggag atgttcattg aggcagtgac tacgattaaa agctttgaac atcggccttc
    31261 tgttcagtat ttctttaaag agatatttcc ggaactattg aaccaggaat tttatggaaa
    31321 agaggcgttt ttagaaaccg tctcaacgat tgtaaagaaa gaagaaaaat aaaaatgata
    31381 tgagatgcag ggggaagtat agttgaaaaa gaatatggat gtcgaatggg gctattctgg
    31441 agacaaaggt ccggaaaatt gggcgagttt atgtgattgg tttgcgagag gtgcggaatt
    31501 tccattgcag tcgcctattc atttgaccaa gggggaagaa accaaaatag aaggagacac
    31561 gctttccttt cattatcaaa agcaacggtt tactgacaag gaattcaaaa acacgattca
    31621 tttagtacct tttgaccaac tcagttatgt agagttcaag aaagaacgct attatttgac
    31681 agatattcat ttccacttgc cttctgaaca tatcatcaac ggaaaacagg aagatattga
    31741 atttcatttt gttcatatga acagcaaaaa agagaatcta gttgtgggtg tcatgtacca
    31801 gctaatggta caggaaggtt ggctgtataa tcaagaaaat ggggaaagtt ggaacttaga
    31861 aaaacatgag cattgggtaa atccggatgt gttctttccg gagcaaaaaa gccatttcca
    31921 ttatattggc tcattgacaa ctccgcctac cagcgggcca atccaatggt ttgtaatgga
    31981 tcatgtagga aaactgaacc aagagtttct tctccccttc gatggtcagt atgctcgacc
    32041 gaacaaccga ccaatccagc ccttgaatgg aagagaaatt tattatttcg aggagtttga
    32101 gcaatgaata ttcagcagat gaaatacgtg gtcgctgtag caaacaatgg tagtttcaga
    32161 gaagcggcaa agaaaatgtt tgtctcccag ccaagtcttt cagctggtat caaagaatta
    32221 gaaaccgagt tagggatcac gctgtttact cgaacgaatc gaggggcata tttaacagaa
    32281 gaagggcaag acttcttaaa tcgtgccgag aaaatattgg tccagcttga tacattagaa
    32341 actcactatc taacgagtga aaagagagac aatttttcga tcgctgccca gcattatgat
    32401 ttcttaggtc ctttgaccgc acagctactc gaggaattca aagacgagat caaacaattt
    32461 cggatcatgg agacgacaac agcgagagtg atcgaagaag tcaaagagca gcacagtgag
    32521 atcggcatta tttatatgaa tgagcataat caagctggta tcagaagata tttacaacaa
    32581 ggagagctaa gtggccgcag cattggaaaa ttcaaaacgc atattttttt aaggcaagga
    32641 catccattag ctgaacacgc agagatcacc aaagaagaat tgttatctta tcctcaagtt
    32701 cgttttacac aggatggaaa taatttcccg tatttttatg aagaccttat cgagatccct
    32761 gatcaagaaa cagtgattta tacgagtgat cgaggtactt tgatgaatat cgttcttgaa
    32821 actgatgctt acgcttcagg atctggtatt gtgattggag ggattcgcga gcacttgcgg
    32881 ttaatccctt tggcagaagg cgaactaaat gaattttata tcatttattc ggcgaaacgt
    32941 ccgctcagtg aaatcgctca aagatttatc aaagagctta ctcggttact ggaccagcag
    33001 agctaagaaa agcagatgag aacgctgttt attgaacacc gttgatcaaa aaagaggggt
    33061 tgtgacagaa ttttgtcaca actcctcttt ttgtagcaaa caatttataa ctaattgctg
    33121 ttgtgggaaa gtacgcaaat atgattctag aaacactcat tgattacctg ttttgtttca
    33181 tagctgtaat tttttcttct tctggtgtca cgataatcgt cttttggttt tcataaataa
    33241 cataaccagg cttggcgcca tttggttttc ggatatgctt tacctgtaca agatcaaccg
    33301 gcacttgggc agagtatcgg tattttgaaa aataggcagc taactcggca gcttctgtaa
    33361 ttgtttcatc tgagggcttg tcacttttaa tgatgacgtg cgaaccgggt atattttttg
    33421 catgaagcca atagtcggtt tttttagctg tcttcattgt cagctggtca ttttgaagat
    33481 tgtttttacc aacaagaatg agcgttccgt cagaagaaaa gtattgatca ggctgagatg
    33541 gtttcttacg tttttgtttt tttaagttct tctttttcag gtatccttct gaagtcaatt
    33601 cttctttaat tgcttcgata tccattgggc cggcaatttc cagttgagat aagacagatt
    33661 caagatattg gatttcatct ttggcttctt ggatttgttc gccaatcagc ttaacagcgt
    33721 tcttcagctt ttggtatcga tggaaatatt tctgtgcatt ttgatttggt gtcaatgccg
    33781 gatctagtgc tattttgatt ggccgatctt cttcataata attaggtaaa acaacttctt
    33841 ttgcgccgcg tggtacttgt gtcagaaaag tagtaagtag ttcgccattt ctacggtaat
    33901 tctcagcgtt ttcggattct ttgagtgttt gttcacgttt tttcaattta tttttatttc
    33961 gttttagttc attttcgatt tttcgaatca gttctccgcc ttgttgtttt gcccgatctt
    34021 tctctgcttt ttcgtgataa tagctatcta ataattgact gagtgttgga taagctgtta
    34081 cagcagatgc ctgttcggac aatacctgat aagcaatcgg tgtgaaaaaa tctttatttt
    34141 ccgtttcgta aaaagtagga ataggctgat ggctgatggc tgagaaaaat tcgtcccata
    34201 caaccatctt ttcattagga cgttccagta atcttttggt cagctcttga gcgatatcga
    34261 aaccgattcc ctgaaactga cgttggattg cttttggatc tagttcagtt tgggaaagcc
    34321 gctgaaagat tttttctttc tcggagctaa atgggttcaa gacctcttgt tttggcggag
    34381 caacatattc tacaccagga agcaaagaac gataagtgtt ttgagagctt ccaatgtgtt
    34441 tgatcgcatc cagtattttt ccagtttctc gattaactaa gacgatcgta ctatgacgtc
    34501 ccattaattc aacgatcaat atgatatttt gtagatcacc caactcgttt cgtttagcaa
    34561 agtggaaatg gatcactcga tcattttcga tttgttcgat gtcttctaaa atagcgccat
    34621 ccaaatattt tcgaagcatc atcacgaagt taggcggatt atcaggattc tgataatcga
    34681 tttgggtaat ctgtacacga gcataacttg ggtgcgcaga taataataat cgatggtttt
    34741 tgccgcgtga acgaatcacc agcacgattt cattttcata aggctgatgg attttcgaga
    34801 tccggccaga tagtaacgtt tcacgcaatt cgttgaccat cgcgtgggta aatacaccat
    34861 caaaggacat ggttcattcc tcatttcttt tcataagtat attcattata acgaaaaaaa
    34921 gaagaaaaac caatttggcc gaatagtaaa gcaaaagtct agaaaaatct tggactaatt
    34981 acataaaaaa cagtgtttct atcgttgaaa tccaaatttt taggtataat aaaaaatagg
    35041 agaaaaagta gaaaagaagg tgtttgttat ttgggaagta gttaaatcga gagtacaaga
    35101 aatgaatgaa catttctcag tgtcattcgc agaaaatcca agtaagtatt ttcgacgtct
    35161 tgtttttctg ctcatcttat cattgctgtt aacagttgca atgtttttat tgccggaaaa
    35221 aatccgagaa gagcaagtaa atagtctcgt ttattcgtcc ataccaacca gcaaattgaa
    35281 accttttgat tacagcaaag ctgatcagaa aatcaaacaa agccgggcga tatcggtgat
    35341 gttcagcaaa ccgaacggga aaacctatca ggacatgctt gatgttttta atgatcctga
    35401 gcttatggaa gaattgaatc gaccgatctt ttattatccg atcgtttatg atgtacatga
    35461 actggaacaa aaatataaca tacgaacgga tgaagtgaca tttattttct ttgatggagg
    35521 aaaagaagca aatcggataa ctgcgggtaa gggaggaata actgattttg ataaggagtt
    35581 gatccctgaa ctgaatcgac ttccgttagc caatatcaaa caattagaag aagaattgga
    35641 tagaacggga gtaacttccc aaaattccga gattgctaat tgagacattg gcgcgatgaa
    35701 acggtgggtc tatatagcgc tatttttaat tatcagaaca agtgatgttt gatcttcaaa
    35761 ataacaaata aaaaattaaa aaatatagtt gacgaaattc aaaaattcga ttagacttaa
    35821 atcaattctt aaggaaaggg tgacctgtaa tgaacttatc aaaacgtatt tcagcagaaa
    35881 acaaaaaaaa ttggcgcagc tggcgtagat aagttgtatt aatggtgaga ctttcccata
    35941 gatacaactt tttagagtgc atttctaaag cttgtatcta tgctagacat gaacattgac
    36001 tgccgcatag attagaatct atgcggtttt ttgtgtaaag acaatgaccg ctttggccat
    36061 tgtctttttt tgtagactgg atacttgcct gattataata aataaaaaga ttgactttgc
    36121 aggaggaaaa aaatgaaaaa caaacgatta ataacagttg tagtaattat tttagtttat
    36181 ttagcaggtg catttattta tggaaaagcc tcaggcgaaa aagaaagtac ttcgacgaac
    36241 cagacaaaaa aaacaacagt tgttggggta ttgcaatacg tgagccatcc ggcattagat
    36301 gagatttatc gaggaatcaa agatggtctg aaagaagaag gattaaaaga aggagaaaat
    36361 cttgagatca aatttcaaaa tggacaagcc gatcagagca agctagccac aatgagtcaa
    36421 cagctagtac aatctgatcc agatgccttg attggaatcg caacacctgc cgctcaatca
    36481 ttagcaaaca cgacttcatc gatcccagtt gtgttaggtg ctgtaacgga tcctgtgggg
    36541 gctggattag tcgcttcttt agagaaacct ggtggtaatg taacaggtgt ttctgatcag
    36601 ccgcctgtta cttcacaaat caagcttgct tctgatttgt tgccaaatgc aaaaaaagtc
    36661 ggaatcttgt atgcttcttc agaggataat tcaaaatacc aagtagcaca agcagaagaa
    36721 gctgcaaaaa aagaagggtt agaatcagta aaatatgcgg tgccttccac taatgagatt
    36781 gcacaaacgg ttcaagtgat gagccgacaa gtcgatttta tctatgttcc attagataat
    36841 accattgcca atgcgatgca aacagtggta aaagaagcaa acaaagcaaa tatccccgtc
    36901 attccatctg tggatacaat ggtggaacaa ggtggactag caacaatcgg gatcaatcaa
    36961 tatcaattag ggcttcaaag cggtaagatg gcggcagaac ttgcgagtgg aaaagaaaaa
    37021 ccggaaacga cgcctgttta tacatttgat caaggggata cagtgatcaa tcaatctcaa
    37081 gctgatcgct taggaatcat tgttccccaa tcgatgaagg aaaaagccaa aatcattaca
    37141 gatgactcgc agcaagaaac atctaaggag gatgacaaat gatcgtatca gctatttcac
    37201 aaggcatgct ttgggcaata ttaggattag ggattttcat gacttatcgg atcttagact
    37261 ttcctgatat gacgacagaa gggtccttcc cattaggtgc tgctgtctgt gtgacagcta
    37321 ttactcatgg ggtagcgccg attgtagcta cattattagg cgtaggtgct ggcatgatag
    37381 caggactcgt tacggggtta ctttatacaa aaggaaagat accggttatt ttagcaggga
    37441 ttttagtgat gtcaggattg aactcagtta ttttgtttgt gatgcgaaca ccaaatcttt
    37501 ctttattgaa tcatccgatt ctacaagaca gcttccatcg ctttgggtta cctgattact
    37561 ttgacacagt ccttcttggc ttcatctctt tgggcatctt gattgctgta ctgctattct
    37621 tcttcaatac agatttgggt caaggctata ttgcgacagg agacaacgaa acgatggctc
    37681 ggtcgttagg gataaaaacc gatcgtatga aaattttagg tttaactttg tcgaacggtg
    37741 tcattgcatt atccggtgcg ctgattgccc agaacgacgg atatgcagat gtaaataaag
    37801 ggattggcgt aatcgtcatc ggtcttgctt ccatcatcat cggtgaagtg atcttccaag
    37861 aattgacgct agcagaaaga ttgattgcta ttgttgtcgg cagtatcatt tatcagctat
    37921 tgattttagt agtaatcaaa ctagggtttg acacgactta tctgaagttg ttctccgcta
    37981 tcatcttagc tatttgtctg atgatcccgc aattgaagaa agcactgaac ttaaaaacgg
    38041 gttttgagaa ggaggcatag taatgactga tatattgaca atcaaaaatg gcagaaaagt
    38101 cgtaaacaat ggaatgaatg aagagaaagt actattggat gaaattaacc ttacaatcag
    38161 agaaggggac ttcatcactg tccttggcgg gaatggtgct ggaaaaagca cactgttcaa
    38221 tacgatcgct ggtacgcttc aactcagtca aggatcgatc tcttttaaag atcagctgat
    38281 cacaaaagat tcagaagaaa aaagagcacg attcctttct cgagttttcc aagaccccaa
    38341 gatgggaacg gctcctcgga tgacagtagc agaaaatttg ctacttgcac aaaaaagagg
    38401 tcaaaaacgg aaattccgtt tacgtaactt gaaaaatcaa aaagaagatt ttttcagact
    38461 atgtcaagaa gtcggtaatg gcctagagca acacttggat actccagcgg gtgaattatc
    38521 tggtggtcaa cgccaagcat taagcttgct gatggcgacg atcacagctc ctgagttact
    38581 attattagat gaacatactg cagctttaga tcctaaaaca gctaaagcat taatgcgatt
    38641 aacaaatcag cggatcaatg aacaaaaatt gacttgtctc atgatcacgc atcgaatgga
    38701 agacgccttg aaatacggaa atcgtctgat cgtactccaa aaagggaaaa tcgttcaaga
    38761 tatttctgaa aaagaaaagc aacatttgac aatgggcgac cttttgcagt tctttgaaga
    38821 agtagtggat tgatttcgac agaaaaattc ctttcagtag aaggacttga aaactcattg
    38881 acatcttcct ttgattcatc ctatacttaa aacaataaac aattaaacat atttgggtgt
    38941 tttgtaggat cacgtagtca atgattttaa caattaaata gaatattctt tgctctgaca
    39001 agtaaacgat gagaaaagaa tttgctactt tgccacctag acgtttcgta ctttttcgta
    39061 tcaaacgcta agtggctttt tttattgaag taagaacaaa ggaggaataa gatgatttct
    39121 ggacaaacca aattagctgg gctctttgct gatccggctt ctcacagttt atccccattg
    39181 atgcacaata ctgcttttga agcaaacaac atcgatgcgg tgtatttagc tttccaagta
    39241 aatccaatga atttgaagca agcagtcgaa agtattcgaa cttttgatat gttaggtgta
    39301 aatctttcga tgccgaataa aacagcggtt attccttatc tggatgaggt aagccaggaa
    39361 gcgcaattga tcggagcagt gaatacgatc gtgcacgaaa agggtcgttt gattggctat
    39421 aatactgatg gtgcgggctt catgcaatcc gtgcaagaag ctgggatttc gatcaaaaag
    39481 aaaaaaatga ctctactggg agctggaggt gcagcaaagg cagtcactgt tcaagcagcg
    39541 ttagacggcg ctgaggaaat cgtagtttat aaacgaaaca accaaacatt cgctcaagta
    39601 aaagaaacat tcgaacaact cagctatcga acttcttgta agataaaagt tcgtgacttt
    39661 gccgatacgc atcaattaag aaaagacttg aaagaaagct gtcttctagt caatgcaaca
    39721 gatatcggga tgggaaagaa aatcaatcag tcaccgctct tagatataag catgatgcat
    39781 gagaatctag cggtttttga tttgatttat gcacctagag aaacacgttt attaaaagaa
    39841 gcaaaaaaag taggagcaaa aggatacaat ggacttggca tgttgatcta ccaaggagca
    39901 atcgcgtttg aattatggac aaaggaaaag atgccgatcc atgtgattga aaagctgttt
    39961 gattaaaaaa ggataataga taaagatctt ttattctcgc ataaacggtc tcaagaaaag
    40021 taatccttta tgtatcgaag catttatttc gttatttttt attaaattat caaaaaatga
    40081 taggcttttg tcgattggtt atcgtatcct ttatttatca gtaagaaacg aggaggaacc
    40141 acaatgagtc ggatggatcg ctataaggac atccataaga aggcgaaacc tctgaagcgg
    40201 gatacgggga gtatttttgc tagaagagaa cgaaaacgaa cgcgtatgga aaatgaggaa
    40261 gatgctaccc gtatttatga aagagagcag cattcatttg aacatttgaa ccaatacgag
    40321 caacagatat acagagatgc accaagagca gaagaacaac aaaaaaataa aaaatttttc
    40381 aatcggcaaa agaagaacaa accgttaaaa cctcgaagaa agcgatcgtg gaaaaaaatc
    40441 attctatcga ttttgcttgt tttgatcctt tattcaggta tctcattttt tattggtaaa
    40501 tatgtagctg aacatgacac aagcttagag gcagcgactt cagaaacctt tcatgggcaa
    40561 aaagcgacga atggtgcgca taatattttg atccttggaa gtgatacacg cggagaagat
    40621 gccgggcgag ccgatacgat catggtctta cagttggatg gtccttccca taagccgaaa
    40681 ctgatttctt ttatgcgtga cagcttcgtt acgatcccag gggtaggaca aaataagatc
    40741 aattcagctt atgcatacgg aggagccgat ctagtgcgtc aaactttagt agaaaatttt
    40801 ggaatcgatt gtcaatacta tgcgaaagtc gatttcaaat cgtttgaaaa agtgatcgat
    40861 gccttattca tgaacggagt gaagatcgat gcagaaaaag atctgaatct ggatggtgta
    40921 gatattaaaa aagggggcca gaagatggat ggacatgttc ttttgcagta cgcacgtttc
    40981 cgaatggatg aacaaggaga ctttggccgt gtcagacgcc aacagcaagt aatgaacgct
    41041 attttcagcc aattgaagaa tccattgaat ctgatccgtg caccttatgc agctggtaaa
    41101 gctttaggat atacatccac agatgtcact gcttggttct tgacgaaaaa cctcttttcg
    41161 attgcacgag gagctggtgg cattgatcga ataagtgtgc cagtagacgg ttcgtggaat
    41221 tatgggcaaa gtgattatgc tggcagtatt cttgaaattg ataatcaaat gaatcaatct
    41281 gctattacag attttttgaa taaatgatca taaaaaagat caagaagaag ctgcgcaagg
    41341 cttttgtcat gaatcaatca aacgattcac tcgtaaaaaa cgctttctta tggtagattt
    41401 aaccaagaga ggagctgttt acattggata tctatctgaa cttcaaaaat gagtcgcgtg
    41461 aagccatcgc tttttatgaa aaagtttttc agactgaagc atcaggtatc accacatacg
    41521 gtgaaatgcc tggaatagaa caaatgaact tgccagaaga agcaaaaaat ctaatcacga
    41581 atgcacggct gaacatttat gatacattag ttatgtttgc agatacgcca gaaggaattg
    41641 gtcctgaatt agttgtggga aataatgttt ctttagtcat ccagacaaga aatgaagaag
    41701 aaacgaagcg cctatttgaa agcttagctg agggtggtaa gattgagatg ccactggatg
    41761 ctgttccgtg ggctcagttg tatgggatgg taactgatcg atttggcatc aactggcaga
    41821 ttaaccaaga gtgacagcta aagatgaata ttttctggaa acatcaagca gcaaacggat
    41881 catcgtttgt ttgcttgatg tttttgtgtc tatttcgact atacggtatc ttttttttat
    41941 tgccagatgc gaaaatatgt tccataatag agaagtccgc aaaaaattaa gtaacggagg
    42001 tcagaagatg aagacggtga tactagctga aaaaccttca caagcaaaag cctatgccga
    42061 aaccttccaa cagcaaaagc ggcatgaagg gtattatgaa atcagcgatc cattgtttgc
    42121 tggtgaagta accatcactt atggttttgg acatttggta gatatggttg ctccaggagc
    42181 ttatgaagag cgttgggcaa aatggtcttt agaaaatttg cctattttcc cagaaacctt
    42241 ccgttacgaa gttccaatgg acaaacgtgc acaattttcg atcgtaaaac gagaattgca
    42301 gtcagccgat accatcatta ttgcaacaga tggtgatcga gaaggtgaag ccatcgcgtg
    42361 gtcgattatt cgacaagcaa acgcattttc taaggaaaag aactatttac gtttatggat
    42421 caattctctt gaaaaagaag caatctatga aggttttaag aatctccaac ctggaaagaa
    42481 ctattttcca aaatataaag aagcgcaagc aaggcagaac gctgattggc tgattggaat
    42541 gaatggaagt ccattatata gcttgctact tcaacgaaaa gggattgatg gcagtttttc
    42601 tcttggtcgt gtacaaacac cgacactcta tatgatttat cagctgcaag aagaaatcaa
    42661 acacttcaaa aaagaaacgt actttgaagg agaaggttta attacgacaa acaaagggca
    42721 gttttctgga aaaatcgtac caaaaaaagc atttaaaacc caagaagagt taacgaagga
    42781 aatagaaaaa cttggagcgc atttaggtaa gcaaaacgga cggattcttg aagttacgaa
    42841 aaaggaaaag aggttgcata gtccacgttt attttcgcta tctagtttgc agacaaagat
    42901 gaatcagcta atgaaagcaa gtgccaaagc tacacttgat gctgctcaag gattgtacga
    42961 aagcaagttt ctaagctatc caagaacgga ttccttttac attacagaaa atgagcatca
    43021 gtatctggtt aaacatctgc aaaattataa aaaattttta ggaatagaag aagtacagac
    43081 aactgagatc aaagcaaaga aacgatatgt cgatgcgaaa aaagttcaag aacatcatgc
    43141 aattattccg acaaagacga ttccgactcc tgcacgcttt gcgaagcttt ccaaattaca
    43201 gcaagcaatc tatttgcaag ttttgcgcac tactgtggct atgtttgctg cagattatat
    43261 ctacgaagag acgaccgtaa tgacgggcgt acagcaatta aagctaaaaa gcatcggaaa
    43321 gatcccatta cagcaaggtt ggcaggaaat cttacgcatc aagtcaaaaa agaaagaagc
    43381 tcagactctt cctcctatcg tagaaaatga agaagcagaa gtagaactga aaagtatgga
    43441 gaaagaaaca caacctccaa aaccctatac cgaaggaacg ttgatcacag caatgaaaac
    43501 tgcgggaaaa acgctggata atgatgaagc gcaagaaatt ctcaaagaag tagaaggaat
    43561 agggacagaa gctacacgag ccaatatcat tgaaaatctt aagcagaagc attacatcga
    43621 agtcaataaa aacgaaataa ctgttacgcc aaaaggaatc actctttgta aggcagtagc
    43681 caatgaacca ttactgacga gcgcagaaat gactgcgcgc tgggaagggt acttgagaaa
    43741 aattggtaaa caagagggga caccatctgt ttttttagag aatatcaaaa aattcatcct
    43801 acatttattg gcagaagttc ctaatcaaat cgacaaggta gacttttcag atgagattag
    43861 cgggacacgt cagataaaag cgatggagaa gaaaaacgat caattaggaa gatgtccaaa
    43921 atgtaaaaag ggaatagtca tgctttatcc taaaatcgct acatgcttga atccggaatg
    43981 cgacttcaaa ttgtggccaa cagtagctaa aaagaagctg actaaaacga atctcagaga
    44041 tttgctttca aaaggaaaga caagtaaagt tgtcaaaggt tttactggta aaaaagggaa
    44101 attcgatgca gttttagtat taaaagaaga tatgacaatc ggtttttctt ttccatggat
    44161 cgaaaacagt ggaacaaaag cagttgatgg aaacaaagaa acatcaagaa aaaataatga
    44221 aaacgaagta gagtaaatga aaacaatgaa atagcggaat atttattccg aaaagtgaga
    44281 atataaatac caataaaact aaaaaatatc agtgaatcaa gcaaaaacgt tggggtaggc
    44341 tgattttata tagatagcaa ggagtttttt tatttgaaaa aagtgcattc atttttaaat
    44401 tatcttatgg gagcaatcac gattgtggct gtgtaccaag catttctaaa gtatcctgat
    44461 tacgggacga agttgttgtc attgacgcac aatcaaacag catattcacc aacgttgatt
    44521 ttatcgactt tgactgcagg agctaaaagc gctacttatt tttttcataa aacgacaagc
    44581 atgctaaagc gaaagaaaca gcgaaaacaa ttgttggtgg tggtagcggc tatgatcatt
    44641 atttattttc taccagttat tacacaagta tttcatcaat attttggtta agaatacgag
    44701 agtttgtgac aaaagtcatg tcacaaactt ttttctttgt cattttttcg taagaaaaag
    44761 caaaataaaa gtatatacat tgattaaaag tatatacttg gatataatgt acttactata
    44821 gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa tgaaaaagta taaaattaga
    44881 aaatctggca atagcgatgt aacaactatc ccaccagaag taaaagaatt catgggtgtg
    44941 cagacaggtg acgcaatcag ttatgttttt caatctgatg gttctgtacg aatgatcaaa
    45001 gcacaagaag aacctgatat tgattcatta gttgattcga tcatgaatca atatgaagac
    45061 gcgttaaaag atttggttga tttatgaggt atctttcgac taaagaaatt atcaaaatca
    45121 acgcgaaagt tattttacgt cactcgccag gcgaaatgat cggtgtaaaa gatgcaaatg
    45181 cattagatat ggccgtcaaa cagccttcac aagcagtatt caatcaagaa ctctatcctg
    45241 aaatctatga aaaagcggcg attttagcaa ttaatttagc taaaaaacat ccttttcata
    45301 atggcaataa acgaactgct cttgttgcca tgttgttgtt tcttcaattg aataattgtc
    45361 cagttgcttt ttctcgtcaa gaagctgttg attttatcat aatgattacc actagttcat
    45421 tggatttcga ttcactaaag tccaagataa ccagttattt acgacaaacc gcaataaaat
    45481 gaacgaatta aaaactttat ttctcaatta gatcagcatg tcattaagga atatgctgat
    45541 ttttttatgt ttaatcatac aaaatagttt tttcataaaa atatggaaaa tatggattaa
    45601 ttttgaaata attgatttaa aaggaataca aaagagtgaa tagtaggaaa tagtggttta
    45661 gaactagtgg gctgattttt tatatatttt agtttacata atatatatta tacaaagtag
    45721 atttaaaaaa tgataaacct ctctcgaacg gttttttatc aaaaatcgtc atttttccat
    45781 caaaatctgc ttatgcaaaa ttataaaaaa attttgcagt ttttcttact ttttttgtcg
    45841 tttaattgaa tgataaaaac cagtttcttt attcagttaa tcaaatatga acgaattaga
    45901 tatgaatcat tgtatttgcc gttgcggaag gattttttaa ttaaccacag ataaacattg
    45961 ataacgattc gcttattgta atcaacagat taaatttcat atgttcatat gtacggatac
    46021 attttcggac cgattaattt taaccattct gtttatttgc ttacctatta gatatcaata
    46081 aaaaactgct taacagctat ttcagctgtt aagcagtttt aggattttta gttcatattc
    46141 attgtttctt ataatacagt gttcaatact tctcttaaag cacttgttga tttgctgaac
    46201 attgcttttt cttcttcgtt gattgataat tcaacaactt cacgaacacc attttcgtca
    46261 acgattgatg gcacacctgt aaagatatct ttttcgtcgt attcaccagt taaataagca
    46321 gatacaggca atacagcttg ttcgttattc aagatagctt ttacgatacg tgcagtactc
    46381 ataccaatac catagtaagt tgctttttta cgatcaatga tctcataagc agcattacgg
    46441 actttatcag caatcacatc taattcatct tgggcaatac gatggtcttt ttctacaatt
    46501 tcaaacactg gtttaccacc gacagttgta tgtgaccatg cagcaacttc tgaatcacca
    46561 tgttcaccta agatatagcc gtggacgctg cgtgggtcaa cttttaattt caatgcgatt
    46621 tctttacgga aacgagttgt atccaatgtt gttccagtac cgataacacg tgatgttggc
    46681 agaccagatt cgttccaagc gatatatgtc aaaatatcta ctgggtttga agcaacaacg
    46741 ataataccgt cgaatcctga tcccatgatt tcttttacga tttggcgcat gattgacgca
    46801 tttgttttaa ctaaatctaa acgagattga ccaggttttt ggttgatacc agctgtgata
    46861 acaacgatat cagcatcttt acattcttca tagccacctg accatacagc cacattttca
    46921 tcgccccaag ccataccatc taaaaggtcc aatgcttccc cttctgcttt ttcttgattc
    46981 acatcgatca atactaattc attagagatt ccttggttga tcattgcata agcgatactt
    47041 gttccaacaa aacctgtacc gacgattact actttacgac ttgttttttt catgaataaa
    47101 cagctccttt tcataactat ttgttacttt catgaaaagt atagtcttgt ttattcattt
    47161 tatcaacatg ttaattataa tagttgttat tataattttt gatggaaacg aattcctgaa
    47221 tacgcataac agataaatga caaatgttta cacctatatc ccctgataat acaaccaaaa
    47281 gtttagaact gaaaataaat ccaaaaaaat tcgctgcaac gatataataa gacagcaatc
    47341 gcacctaaaa caattttcct tcatatgctt tagtgaaaga ttttcttctt aatgtttatt
    47401 tctccattca ttgggagtac atgttgaata cttcttgaat aatttgaata aagaagtcgt
    47461 ttgtttatat ccgatacgat tagcgatttc ttccaacgaa aaactcgttt cttgaatcag
    47521 ttccaacgca aagtcgaatt ttttctttag aaccagttct tgaaaggaag catcaaaata
    47581 cattttcaat ttattactaa gatagaccgg attgtagcca aaatgagctg ctgtttcttt
    47641 taacgtacaa gaagtaaaat gcttatcgat ataatctaat actctaagca tctcattttc
    47701 tgcgcatcca tctgctgatg aaaagctttg ttgttctaat aaatgagtca attcgatcag
    47761 taatagtgat gtatagagat ttaacgaacg gatcttatga ctgtatgtat caaaataaat
    47821 caacagcata ttttcaatca aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat
    47881 gaattgtgta tgttcccctt tttcttttga tgcatttatc aaaaaagaag aaagtagatt
    47941 atttgtgctt gccaaatgat tcaatatctc tgtagataag gtttcttctt ttaataagat
    48001 gttgattaaa atatcctctt gccctgtgta ttcgattctt tgagtagctt ctttatcaat
    48061 gatcaccacg tcactttctt ccatctctat cctttctcca ttaataattt gtaaacatgt
    48121 accgctatac atataattga tttcaacaaa atcatgtgta tgctcgggaa cttccgcata
    48181 tcgacggtgt ttactgatat gaatatttcc ctgttgaaat agatcccctc ggtggatttt
    48241 tggcggtgtc tgaagatggt aagctaagtt taaatcattg atattcattc cttgttcacg
    48301 ctgcagagtt tcgattgatt cttcgttacg caaaaaaaat gtctaattca gctagttcca
    48361 attgactccc tcctttttgt aagcggtttc tttcgcttat tagttcacgc tgtactgcta
    48421 ttatatgcaa agctattttt ggtttcataa agactttcta tggttgggac atgaacttcc
    48481 tgtgattccg ctattgagag aataaccact tcatactata tttttgataa gcgcttacaa
    48541 aaatgaaagg aacatgctta tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc
    48601 tcttattttg acttctgctg gtgcagtcac gtctgcaatt cccagtatgt cagctactta
    48661 tgccacgcgt tctttatctg ctgttgaatt attaacgact gctccctctt ttatggtcac
    48721 tatttttgtt ttgattagta gttttattgc taggaaaatc ggacaaaaac aaactgtgat
    48781 catcggatta gtgattgcat cgatttgtgg tatcctcccc ctcttctcac aaagttacag
    48841 tattgtctta gcttcaagag ctggactagg tattggcttt ggattgatca attctttggc
    48901 agtcagcatg attgctgcct tttatcacgg agatgaacga gcctctatga ttggttttca
    48961 aagtgcattt caaggatttg gtgcagcttt aatgacattt gttgcaggac agctagtaca
    49021 gttcggttgg caatatacct ttttagtcta cgccatcagc ttacctattc ttgtcttgtt
    49081 tgttttgttt gttcctacac ctgaaacaac tgaacttcaa tcgcatggca cagccccaac
    49141 aagacaagta atcaaaacga atcctaaaat gattagttat ggtatttttc tcttgctggt
    49201 catcgtggta tataatgctg tatcgattaa attagccaca gtcctgacga cttattctct
    49261 aggaaacgaa actaatgcag cgaatatctt aacaatcatg caattggcaa gtatggctgc
    49321 cggagtttgt tttggctggc ttcagacaaa agcacatcgc tatatgctta cactttcttt
    49381 actactgatg ggaatcggct ttgtattgat tgccactgtg ataaacctgt atgtgattgg
    49441 gtttggtgcg atcctaacag gaatttcttt ttcgttattc attccttact tattcaacca
    49501 agtaagtatc caagcgccca agtacgcgga gagtttcaat acttcaatct tattggttgg
    49561 cgctaattta ggtactttca ttgcaccata tggtttgctc tttctctctt ttgcttctct
    49621 tgggagtaaa acacttgctg ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct
    49681 tattagcctt ctagtagtac acaggacgaa tagaaagaca gtgataagag tagatgaaga
    49741 aataaaagaa caattgacaa caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga
    49801 aaaacataaa gatagatggc tttggtatcc tggagacttt gagatccgcc atggactgct
    49861 acaaaatttc caacgggaag aacgaggttt tgactggcca gcgtactggt acatggatga
    49921 ttgccatcgg aacgtcaagt ttaagagata ttactttctt gatcaacctt ccatgtttaa
    49981 agtgacgatc caaggagttg ggtatgtaga aattaatgga caaaaacatc catgtggaaa
    50041 atggctgact tgtccagctg gtaaagcaaa aattcgtatc tttgtaggac atacatcagg
    50101 gctaccagcg atgtttatcg agggagacga agtaaaatca gatattggct ggacagcaag
    50161 taattttatt gaagaatatc cagctggctg gagtccgctt tatgtcgata tcgcaaaaga
    50221 tccaaatcaa atctattatc aaaaagaatg cgtccatcca gtcactgaaa cggagattgg
    50281 cggaggaatc ttattcgatt ttgggcgagc agtgaatggc tgtgtagaac taatatctct
    50341 ggatgaccat atcgaatcta tcactctatg ttatggagag tccgatgtag aagctatgga
    50401 tatcgattat tgctactata agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg
    50461 cgcttttcgc tatttgttta taccagaggt ttctactgga atggttcaac ttcaagtttt
    50521 tcatgaattt ttatctcttt cgaaacaagc tgctttttca agcgattctt ctttactgaa
    50581 tcagatctgg tcagtatcag aagaagcctt caagttatgt agtgatttgt tttttatcga
    50641 cggtatcaaa cgagatcgct ggatttggtc tggagatgcg tatcaatcat tcatgatcaa
    50701 tcaatatctt ttttttgacg aggaaatcaa taaacgaacg atattagcat taagaggaca
    50761 aaatgaaatc aagcagcata tgaatactat cgttgactat tcgatcttgt ggttgattgg
    50821 tatcgaaaac cactatatga tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa
    50881 gatgcaatca ttaatgagat acttgatgga gcaaacgaat gaattagggt ttatttatgg
    50941 gagagcaaaa gattggatat ttattgattg gtctgaaatg gataaagaag gaacgcttgc
    51001 tgcagaacaa gtcttattac tgaaagccta tcattccatg atcacttgcg gagatatctt
    51061 agctgaaaca tatggagaga acgaaaatta tcatactcat ctctatcaag aaaaatatca
    51121 actattgaaa caaaacctat ttgactactt ttgggatgaa gaacttggcg cttttatcga
    51181 ttgctatgaa tctgggagaa gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt
    51241 cgattttgct aacaaagacc aacagcaatc gatcttaaag aatgtcttat tgaatgacac
    51301 tatcacacag attactactc catatttcaa attctatgaa caagatgctt tatgcaaact
    51361 cggacagacg aatatcgtct accaaacgat tcttgattat tggggaggga tgttatctca
    51421 tgatgcggta actttctggg aagaatacga ccctactcaa acaggaaatg aaaagtacag
    51481 tatgtatgga gatccttatg gaaaaagctt atgccatgca tggggagcaa gcccaatcta
    51541 tcttttagga cgctatttta tcggtttgta tccaacaaag ccaggttatg aagaatttat
    51601 gatacaacct aatctagatt catttgaaac actgaattgt acgctaccta tcaaagatgg
    51661 aaagatttcg cttgaaaaaa caaaagataa gatcgtgatt acttctgata gaacaggcgg
    51721 aacagtgaaa atgaatggta aaagttatcc tcttctagca aaacagccac ttgtcttaac
    51781 aagctattca aaaataaatg cataaaacaa aaaactgctt gcgacatatc agaagacttt
    51841 aaatcttctt ctgatattgt ccaagcaatt tttattttga agtttgctta attctgttct
    51901 ttagaaacaa tgtttgttct ttttccggca aaaagaaagc tagataatac gacaagtaaa
    51961 aagaaagcga tgattccaac tgtcaaggct atttgaccgt attctcctag tttcccaagt
    52021 acaagtcctg gtactagata atcagtatcg gcaaatgtag aagaagcgat tttaagatct
    52081 cctaatactg gaaggataaa taatggcagc caactaatca ataagctatt gaaagcagaa
    52141 cctaataccg cccctcttct accgccagtc gagttgccga agacaccaga agcagctccg
    52201 cagaagaaat gtcctactac cccaggaatg ataactgtag tttgcaagcc tatcatagca
    52261 aacatggata gtgttcctac aaagaagcta acaaaaaagc caattaatac agcattaggt
    52321 gcatatggaa aaacgatagg aacatctaat gctggtttag aatcgggtac gagtttttta
    52381 gcaatccctt ggaatgctgg aacgatttct gccaacacca ttcgaacccc ttgaaggacg
    52441 ataacaaacc cagctgtgaa tgttccagct tggatcagtg caaagattac agcgttgtct
    52501 ccattgctta gttctctttc aacaaatcca ggaccagcta acaaagcaag tgcgacatat
    52561 acgatcgaca tcaagagcat gatcgataca gtagaatctc gcaaaaagct gaaactttta
    52621 ggaatcttta tgtcctctgt agaacggtct ttgtttccaa aaaactttcc gatccatcca
    52681 ctagaagcat aaccgatatt tccggtatgc cctaacgcaa cttgattgtt tccagtgatg
    52741 ttcttcataa acggttgaca aagagcaggc gtaatagtta atagtgttcc ttcgaagacc
    52801 ccgcctaata agatcatcca caaatttgca gtaaaccccg cgcttactaa aatgactgct
    52861 gttagacaag aaacataaag cattgcttga cctgtaagaa aaatgaattt atatcttgtg
    52921 aatctagcaa gtaaaatatt cacgatcatc cctactagca tgatcaaagc agtaggtgta
    52981 ccgtattgtt gcaaagcaat cgccactact gcttcattgc taggaactac tccttgagta
    53041 tgcagagctg tttggaacat cgaagcaaaa ggggttagtg aattactgat taaaccagca
    53101 ccgccgatca gtactaggaa tccgacaaat gttttgatag ttccttggat cacttctgaa
    53161 aatttcttct tctgaagaac gagaccaagc agtgcaatca aagaaacgag gatggagggc
    53221 tgactgacga ttgacacaaa caaattcaac aatttatcca ttgatagttc ctcccttgaa
    53281 tcatttttat tcttttgaca agatcttcgt tactttttct tttacttctt cgctgtcaat
    53341 gatcgattgc aaaggtacga gcttttcatc aggtatagaa gttagcgtat ctttcaaaga
    53401 atgctcaatg aagaaataat cagccatatc gctagataca cttccaacat ccgtatgact
    53461 tacttcaaac tcgtccacat ctgctccaag atctgcaagt gcttgtttga tgttcatctc
    53521 taccataaaa cttgttccta atccactttg acatactgca aaaattttca tatcattcgt
    53581 ctccttttct tatgatcgct aatatttctt cttttgttga tgcgtgtaat aaatcttgaa
    53641 gattttgttt attagaaagt agctttgtta aatttgccaa agcccgaaga tgtgcctcat
    53701 tatcgatagc agctaaacag atgaacaaac taatgggatg ggctggatta tcaagcagat
    53761 ccacaggttg ttgaagtttc agcaaagaca tccctacttc gtttacgcca tcttctggac
    53821 gagcatgagg taatgcgatg tgatcgccaa tatggataaa cgcaccatac tgctccacgc
    53881 gctcgatcat agctttgata taactttctt ctattttatt ttctgataat agcggctgtg
    53941 cggcaaatgt gattgcttct tgccagtttt ttggttgatc cgataattgg atcatttctt
    54001 ctgttaataa ttcagatagc ataggacgtt gatcctcctt cttttctaaa agtttgttca
    54061 ttttacgatt taatacttga tagagctttt cttcgtctac gccttctttt aattcgatat
    54121 aaggcagtag cgccgacaac aattcttggg cagaaggcat tgaaaatcct ggtatcagcc
    54181 aatctctttg tacttgattg atcagctgat ttttttccaa gtcagtcatg attggatgca
    54241 gaacatagag agactttttc ttatcagtaa gtgtgagagg tacagtagaa aaaataatat
    54301 cgtaagaagt ttcgtcaacc tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga
    54361 tcatcgggaa taattgctga agttcacttt ttaagatcaa tgaagagcta atgccacttg
    54421 gacagacgat cacggctctt atttttctat ttctttcttc tgcgtccgct tttctgatct
    54481 ctccgccaaa tagtatagtg aaaaaaccaa tctcttcttc agggatgctc ttccctattc
    54541 gtctttctaa cggaagtaat gcttttcttg tcatttcgta gatggaagca tattgatgct
    54601 taatttgatc gatcatcaca ttaggtaaat aaaaagaata ggaaatacga aaataggcag
    54661 ggaccaagtg ataaaagaca ttcatcaata attcacgaaa cgaagcaaat tctacggcca
    54721 tcaggaattc catgcgatgg atgacttctg aagaacattc cagcaaaaaa tcaagtgcag
    54781 gctcctggac atctccttca acgatcgatg ctaagcaaag agagaaaaat ataatctccg
    54841 aattttgatg ttcggaatag ccaaggccag tcaatacttg tcgactaatg tcagtgactg
    54901 caagagaagc caacagttca gcatctttta ccagttccat ggatgttttt ccaattcgaa
    54961 tggttgtcaa actcaaataa taagctaatt catccattcg atttggtgcg aatcgaagat
    55021 ctgatagtga aatcgtttta tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa
    55081 gggttgcggt taaatacgtg agcccaaatc ttccagcaag tgactgcatc aaacgagcag
    55141 tgcgattttt tgcttctctt cttaatattg cttctgaccc tagcagccta aaaccagttt
    55201 tcctagtata cgacagctga caatcttcca cttctaattc atttcttatc tttttgatat
    55261 ctgacaggac tgtcccttta gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag
    55321 acgttgcagt cgaataaacc atcaaataaa gaagatctct tcgctcttgt tcggtataca
    55381 cagcttcaaa tcgagtaata tccaaaaatt tttctgccca gtcgattgct cggaatttct
    55441 ttatacggat acgggtatca tccacatatt cggcaaaagg agttaattgt ttgatcgcct
    55501 cgcttgtatt tacttcgatg atctctgctg ctttttctag actgatttct cttggtgttt
    55561 tcaggagata actaattttg tagttcacag cgctgttcac ctcctactta tattctagta
    55621 tggtagcgct tttaaatcga ggggtgtttg gtacaactta cagttggatt ttttttacga
    55681 ctgtaccgaa cgatttctat cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc
    55741 tgcttttatg aattgatttc gtccgatcaa tgatgatcat gtttattttt tgaggtcagt
    55801 tcttcaaatt tttctttgac atcttcaacc gctttttcga tatctttttc aaagtgattg
    55861 ggctcattag gctttgtttc ctcttctaca gaatttgaac cagataaagc tgtttcttta
    55921 ttttctgttt caccagagtt tgtgccagct aatgattctt ctttgtcttc tgtttcacca
    55981 gaattagttc ctgctaaagc cacttcttct tctgttttag gggccattga acctcttaat
    56041 cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc ctcctagtag ttctatactt
    56101 tttcatcgta caataagaca gtttatgcgt caaaaaacaa acccgattct tacaaatatg
    56161 tcatattttt ccttctgatt cttgaatgct tttgattcct tcttccaaaa ttttcatttg
    56221 taagattgtt tcttcatctt tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat
    56281 cccttcgtag acacggctgt aatcaccaac ctcggaaatt actttttctt catgataact
    56341 cccttgttca tctatatagg tcaatacacc ataatgttcg ggcaaatcga taccaaagtc
    56401 ttcatgatca ggcatgtaaa acattttcag atgttcttct tgtcgatctt ttgtttgttt
    56461 cacgaacatc cctttttttc cgtaaacgac gaaacttgga cgttctttga tgcggaaata
    56521 actggatttt atcgaaattt tcatatttcc atagaaaaaa tccagatcga agtaatcatt
    56581 cattcgtcct ttccctaaaa gttgacgtac atcatagctg atatcatctg gttcgccgaa
    56641 ataagaaaga acttgatcta ctgtatgaca agcatgtcca tataaatagc tgttcgcagt
    56701 agaaaattca tgcgtatttt ctggtacttc cggtctgaaa taatcataat gcatttctac
    56761 ttctaaaata tcccctaatt ttccgctttc gattactttt tggactgtca agaagtcaga
    56821 atcgaaccgt ctgttttggt aacattggat aaatagacct ttttcttttg ctaatgcaaa
    56881 taactcttct gcttcctttg acgtttctgc aaaaggtttc tctactagta cattcttacc
    56941 atgttcgagt gccatttttc catattcata atgaaattga ctaggtgtag tgatgacaac
    57001 taattggatc tcaggatcat cccaaagatc agacagctga tcagtatatt cgatgtcttc
    57061 gaatgttttc cacactggtt cagatgggga aaaaattttt ttgatattga ttttgttcgt
    57121 aagttttctt gaaaaaggca aatgatagcg atttgtactc tttccattgc caagataagc
    57181 gattgtaagc atgagtggtg cctcctttta ttgatgtttt cagtatatcc tagaaaagcg
    57241 cagaaagtat ttataatagc tattctggta aaaaagttca gaaaaacact tgttatcatt
    57301 cggtttggtg aaattaatgt tcaaagtaag gataaaaatc aaaaagatag tagcggaacc
    57361 aagtgtttat cagtaagata gaatttggag gtcagatcat gcttattgaa gaaaaactaa
    57421 caaaacaaga gttgtttacc acgacagaga aacggattgt cgattacatc cgtaggaata
    57481 tcgaagctgc agtctacatg acgattgaag aattagcaaa agctacgtat acttcccatt
    57541 cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg
    57601 agctggggcg ggaagttcat caattgattg ctcagttcaa tcaaacgatt gatcctaatt
    57661 ttcctttttc aagtgaagat agcactcagc aaattgcaaa gaaaatggcg gatttatcga
    57721 tccagtcaat aaaaaaagca caattgcaga ttgaacatga agatttagat caaatcggta
    57781 aagtattgaa caaggcacag cgaatctttc tttttgcaaa aggtgattct caaatcacgg
    57841 caaggaaatt ccagaataaa atggtcaagt tgaataagtt tttgatcatg gctgaagaat
    57901 acagtgattc ttcttggaat gcagccaatt tgactagcga ggattgcgcc atttttatta
    57961 gctacagcgg tcgcatccat cattatgaac gaataatgac ctatttgaag catgtagagg
    58021 cccctacctt gctgattacc ggaaatcagc attcagaaat ggccaaacag gctgatatgt
    58081 gtcttgttat atcgcaggag gaatatgatt ttgctaaagt agcgactttc tcctctcaaa
    58141 ttgccttcga ttacgtatta aatacgttat tctcagtgat atattctcaa aattttgaag
    58201 agaatatccg caacttgaaa aacaaacaag aattgatgca aaaaggcctt cttaaagaat
    58261 atcggaaaac ttaagaaaag atcctcattt agtaataaga aagatagtgt ttgataggct
    58321 ctctttgata tcatcataaa aacaggctga agttttcttt ttcaaacctc agcctgtttg
    58381 tttacggaac gattcgaata gatatattat agaaatgagg aatagctaac ttctgtttct
    58441 tattttgaac gattgacgaa aaagatatat aagaaggagg aaaatcagta tacatgcagc
    58501 taacaatcca tacaggaaaa atcgtttgtt ctcggatact ttctgtactt ctttttgaac
    58561 ttcttttgca tgataaggaa cccggtgccc tctgacaagt aagcgatgac tgttgaccat
    58621 ataaggtgta caagttagga gtgtcacgaa atcctgccct ttttcgattc tcagatcttt
    58681 tgtatcagtc ggttctaccg tcttgatctg atcgacttga taagcgagtg tccgtccatt
    58741 gatttcgata tagaaagaat cacctaaagt cagtttcggt aagtctgtga acaactttgc
    58801 ttgaggcagt cctcgatggc tggaaataac ggcatgtgta ttttctcccc ccgtaggata
    58861 agacgttcct tccaataatg aggcaccttt ttctagcagc acagtattcg tttcatcaaa
    58921 aattggcaaa tggacattga tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa
    58981 ataggttcta tcctttttcg caggttcttt tttcttagta aagggatctg cacctggatt
    59041 tccaccttct tttgctaatt gctggttttt ctttgtcatg ttttcttgga ttttagccat
    59101 gacttcttca ttttctttga ccgcttgttg ctgataatga gagatgatct gctgatccag
    59161 ataattattc agcgcgtcgc taacaaatgg ataagctagt atccctattc ctgagagaag
    59221 caagaaaagc atcagcagat ctagaagttt ccttttcttt ggtttcattt gtgttctcct
    59281 tgtgatgact gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt
    59341 tccaattctt gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag tacagtccag
    59401 caatcagtag aaggactact cctgcaccga tatacacata gattccctta ccgcctgttg
    59461 aaggaagtgt acctttgtgt ttatttggta ttttttcagg agaaatcaac tgtcctgctg
    59521 ttacataaga ttgttcatcg atagtaaatg ctacacggtt tgttaatgga acatattttt
    59581 ctggcgcttt cgtttcttcc agatagtacg tgccatattt tagacctgtc acatcgatta
    59641 aaccgttact tgtggttgta aaaaccgttg ctgattcttt cgccgatacc cagctgacgg
    59701 cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc actatcttga tcacgaacga
    59761 cgaattctgc tccagcaagt gtttggtctg atgtaacgtc accatctact ttaacaaatc
    59821 gtttgccccc agtaacgaca tcgactgacg gtggtgtttg atcatttgta tgcccgttat
    59881 cgacatttgc ttggttagaa aatcctttgg ttggatctgc tttttcgttc aaatgcatat
    59941 agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg aatataattc ggatcaactg
    60001 aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt tactgggtcg atttctttat
    60061 ttccatcata taaggcataa gcatacgttc ctgaagaatc attatcaaat gttaaagcag
    60121 cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag
    60181 cgatacctaa tgggatattg acagaaatct catattgaat tttttcaccg attgcgacat
    60241 cttttccatt caattgaggt gttgttttat caactttaga cgtatcattt ttgatggtct
    60301 tttctactgg tgtttggcta tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa
    60361 tcatttccgc attatcagga gcttttactt cttctaaatt atattttcca acttctaaat
    60421 gattaacgat caactggcct ttttcaatag atgcctcggc aaagtcattg ttgccgatgt
    60481 cataagaatg accagtaatg aaatgtttgg ctttggtttg atcagttgtc caagtgtaca
    60541 atccatctgt gacactttgg atgtattttt tgacgcttgg tgttccttct tctttagaaa
    60601 taataaattc tgctccattt agtgcttcgt tttcggcagt accgattttt gtaactttca
    60661 acgttccatc attaccgact gtatttttag ggtagagatg gatagtatct agttcttctg
    60721 tcccgtattt ataagagcca tctgcttgtt tgatcatctc atatacaggg aaagctaaaa
    60781 ccatgtttgc ggcagctgac actccgtctt ttggttcttc tttgatcgtg tagactgcat
    60841 ctttcccatt ttgtttttta ggtaaagata aagtgacatt tccatcagca tctgtcgttc
    60901 ctgaagcaac tggtgtacca ggagtcaaag actggactgc ttgttttgct gcatccacgg
    60961 acgctccttt atctcgttgc gcataaaatt cttgagtgac gttataaact gaaaatgaaa
    61021 tatcggctaa tccttggtat tgatcgaatt cgctcatttc tttcccgctg ttttggatta
    61081 aaggatcggg taaatcagtc attttctttt tatgaaggat gacttgagct gctgtctcct
    61141 ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga
    61201 ctcctaacat aacgtttatt tttttatgat ttttcatttt ttcactcctg ttcttttttt
    61261 gataaacaac cagattgcaa agcttgcgaa taccatacca acaacgccta aaagtgtatt
    61321 tttcgtttcg tttgtttttg gaagaaatgg acggctattt tcttttttat tgactacttt
    61381 caatatactg tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa acggaatttc
    61441 tgtttcactt aaaatataac cagaaggtgc ttcgatctct tttaatcgat aacttcctgt
    61501 tttcaacccg gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa gcgcatctga
    61561 ttcgttcttt gtccacttgt atccgttggc tgtttgctgg aggtaatttc ctgctttgtt
    61621 tttaataagg aatactgcgc cagctaatcg tttatctggt ttatttgtct cgattttgac
    61681 aaattgctta ctccctgtat atacagagac tttctttgta ctgacaccaa aaccaaaatc
    61741 cagtgttccg ttgttgttga tttccttgtt cgcctgtgct gtactgctta gctgcatctg
    61801 ataagaaatg gtcagttttt ttccagcata tttttccaac gaagggatcg aaaataaaac
    61861 atcaaatccg tgaggtttct tgtgtatgac aaagccttct gtcagctttt ctccattcga
    61921 tgaaatcgtt aaactattag gtaaaaacgt taatgcttca tccgcactat ctgacaattc
    61981 gaacttttga tagtcaagga tatttacagg aattgtcgta tgcaaggaat aagtgatttt
    62041 ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag gtggatcagg
    62101 gtagtcgttt tcttcatttt taggatacaa atggatagtt gaaagttttt gattgttttg
    62161 tccatgtaca ggaagaacaa ctaccatatt ctctgccttt tctttgacga cttctggtac
    62221 tttggattca atgaaaagat acgctttatc tcttttttct tgatctttag cggccagaga
    62281 aaaagaagcg ataccatctt cgttgttaag agttgtagtt gttgcttctg cggtaaacat
    62341 accggaagac gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt
    62401 ttcccgtaga tggtaaaaag aatctgtgac atcataaact tggaatgtga caccatttaa
    62461 tcctcgatac gtttgtaata aagctttttc ttgtccgtca tttggatgat ttttcggcag
    62521 ttgcccgttt gggaaaagca atttgtgtaa agtaatctga accatttctg tttctgtttc
    62581 tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag aagagacaca tactaagcca
    62641 accaagtttt ttcatattta gctgccccct ctttcttttt tcaaaaaaag ataccaaata
    62701 gaaaacgcac aaccaatcat ccctactaga tagattccta aacggccaat tcctcccgtt
    62761 tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag aaatctggtt gtttttggct
    62821 cctggtgaca gaacagattc atgatcttgt ccatccactt gaattgaccc atcttcgtgt
    62881 ataactatag taactggctc ttttagacct tggtatcctt ctggtgtaaa agtttcggtc
    62941 agcgtataag ttccaggttt taaattttcg aatagaaagg tatctgtttc ttgtccatct
    63001 tttggcgatt ctaagtccat ttctggtccc tgcagtctga attttgctcc ttttaatgtc
    63061 tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg cttttagtcg attttgatga
    63121 gtgagcgtcc atacattatt tgtcatctcg gtattttttc ccgtgtaaat gatttttcca
    63181 tatttatcga tttggaattc ataatttccc ggcgaagtat catatccggc aggaccagct
    63241 gtttcaacga ctcgatattt accaggtgtg agtgaatcaa aactggcaag acctttttca
    63301 ttcgttgttt ggctgtcaat tggctgataa gtaccatttg cttctttttt ctgtagggaa
    63361 aatgctgctc ctttaagatt tatctctttg tccgtccctt gcatcgcata cttacgtact
    63421 gctacaggaa cttcaacaaa aggattttca atagtcaact ggatcgtatc atcggaagta
    63481 gtgactgttt ttccatcaat ggttaccgta ccatcagaag cgatcttgat ttcccaagta
    63541 gtctttttgc ttaatccatg cccttctgga gcttttgttt ctgtcagcgt ataggtcatt
    63601 tctttttgca atttgacatt ttctggaaga gaataggttc cgtcgccatg atctgttagt
    63661 aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc caataagatc cttttcacct
    63721 gtagaggaat ttttcgttat tttcaagttt aacggtgtga attgtttagt atttttccat
    63781 gtcattgcat ctatttgttg agaatcgtat cctggtacag gtaattcttt gattgtttgg
    63841 taactgaatg cttgaccttg attattgtat tgaggtagtg ataatatctc ttgataactt
    63901 tctccgctat ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct
    63961 ttagctggtt taatgattcg aatatatccg tttttccaag ctgcagcatt tgtcgtatgt
    64021 tcccgttgaa tctcaaaagt aacttgatct ggacgatcag ctagattgtt gtcaaattct
    64081 tcccataact ttttgatgtg aagactgact ccgggagctt tagcagatgg tataccgaat
    64141 tcagctaatt cattggtatc aatgtttggc tggaaagttg tcctgccgtt catttgatac
    64201 cagaaatttg gatggaagtc ctcattttct gtttggattc tcacttggta atggatttgg
    64261 atttcttggt cttttcctaa atagatctga ttgctcttga tggtatttcc ttctatggaa
    64321 atagatggag tgaccgttgt aggacttgtc cccacactct tgactgaaag tgtaccaggc
    64381 tgataaatga atggttctgc gattggatca tttatttgtc cattgcttac agttgctgag
    64441 atctgtacag cttttttggc taggtattca gagatatctg ttgcatgatc agctgattca
    64501 tagtaaagat cgcctttttc atctgatgaa accatttgtc gcatacgaga ctctacttct
    64561 gcttttgaga gaccagctgc cggatcgctt tgaagttgga tgccaagacc atgtatttcg
    64621 attcctcgtt ctttgagtgc catcgcttct ccgatggttg cgataaacgt actgtcgatt
    64681 cttctggata gattgttttg gtcaggtgca tcatagattc ttgagattag agaagtattt
    64741 cccggccgat cttgcgtatt agaaaactga gttccgtaat aattgctgct tgattgtgcg
    64801 tgtacccgct gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact
    64861 tttttatgtc catttggaac ggatagcatg cttcctgcat ctcttagtgc tttttgtgta
    64921 aaagtaccac catttgtccg tgaaggtgta atggatttta cttgattttt cactgaatca
    64981 aatgaaccca tctgtactgc accgttactg tagctatatc cttcgcttga gtagccgaca
    65041 tatcccatat tgattttgtc tgtgataccg ctatctgcta aagtatcgac aaaacgatcg
    65101 acaccaatct ttacttcacc gatccgatta ttgtcgttca tacttcctga ccagtctacg
    65161 accaatacga gatcaagagg cgtgatatct ttttgtacat ttccacgagc attcaaataa
    65221 acatcaaaca atccgggtgt acttgtttcc tttgcatact ttcgtatcgc atagtctgct
    65281 tgattaccgg ttcctccata ttcgatatac gaatgggtcc gatcattagg atctccattc
    65341 caagaattta taccatccca ttgatttgtt ccgtttttat tgccttgatg atcaagaaca
    65401 tttgtatttc ctgtaggttg ccaactggct ttaggataag tgccctgatc atctgttgta
    65461 tattctgtag gaatcgtttg attagatggc acactcacag ctggcaagga taatgtaggc
    65521 aatgtaaatg ggctgacaga attttcgttt tcttttgatg attcttcttc tatttcgtct
    65581 ggctttggta ctgacacatc tgtatctttc ttattttctg tctcatcaat aatgtcatat
    65641 tctttattat cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg atcgattgga
    65701 aaactcagat cgatagtttg tttagtgttt gatgagattt ccaacgtaat tttcccgcta
    65761 tttacctgat aagcatagcc atctccttct tttctttcca catcacttaa gtaattttcg
    65821 actagaccaa tttctttttc tatcttttca gcactggtgt tattcgcatt tagcttgatg
    65881 ttcagtgctt tttttccaga ctgatccgaa atgacttcag ctgaaattgt ttctaaaact
    65941 gttggatcat ctgtagtgtc tgctattgca gataatggca caaagtttga taaactcaat
    66001 attatcagca tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt
    66061 tttctttttt tctgttttga cgtgaatagt gaaaattatt aaaataaaca ggcgttacat
    66121 aattttattt attttaatat gataattatt atacgaaggg cgctaaaggg aatcacacca
    66181 attttcatac caattggttt catttatgaa gttgattcct tcgattaatg agcgtttcat
    66241 tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt tgttggaacg aaactataga
    66301 aagatggaaa aaatcattcg aattctgtcc atttctgaca agtggatgac taaaaaagaa
    66361 cttgccaaac gaattggtag ttcagaatct acatttgtaa ggtatatcga agagatcaaa
    66421 atccgctggg gaacggatgt aacgattcaa tcttcacaaa aattggggta tcgtcttgaa
    66481 catttgaatg taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac
    66541 agtcgtttat taaatgagat tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa
    66601 aaaatttcca tcagccggtc ttcttttaca cgtaagttga aacaatgcaa tcaaatttta
    66661 gaaagctatt tattaaaagt gatcgttgac caaggttttc agttaactag ccttaaaagt
    66721 gagctccagt tgcgtatctt tgttaccttt ttctttttaa gttactacgg aagacgcacg
    66781 ttgccatttc caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa taattgtcag
    66841 atgaatttgt ttcaggaaga aagcccatat gagcaatctt tctttattat gtattttgtt
    66901 gtgcatctca tgagagaatc ccaaggaact ccatttgaat ggaaagctgg agactcttat
    66961 gtacagttga taaatatcga taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa
    67021 ttgaagtaca gcagctatat aaaagtagtg aactattttg tcaatacgat tttttcagaa
    67081 ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat gtcctactgt
    67141 tcgttttcat ccgctgattg caaagaaaaa aacgaagaat atgttgttca attattaacg
    67201 caaatttatt ttttctcccg tattttccca tacgatacga ctcatttgac gatccgtgca
    67261 acttactttg cacagcagat gcaacaccgc cagatacgtc tatatgctgg atttcgtgca
    67321 gaacttcaat tttattccac tctactgaat cagaatctga tttattattt atctagctat
    67381 atcttttgga tgcttatcgg taatcctgaa attcaccatt atacttctga taaaaaaata
    67441 ctgattatca gcgacttaag tcttcgacat agtcaatata ttgaagaata tatcagtgat
    67501 attcttgctg tgcataaaat tcactctgag acaactgcta ttactgaaga tcagctgtcg
    67561 aaatacaatc ttctcgagta tgatctgatc gttacgaatc aacccatttt gaatgcccat
    67621 gttcctcata tattgattga tgatagtgtg tcattcgcaa atgaagaaga actcatccgc
    67681 ttattcgagt tatgaataat caaaaaacag ctatgtataa accaccctga taaaaatttc
    67741 aggacaaata cacatagctg tttcttttta ttgtattcct tcagcaatcc aacgagattt
    67801 atagcgaaag tcttttaaca actcttcttt tgtccaatta tttgcaatga gatccgtcaa
    67861 agtgttcatt acgagggtca tggaaaagta taggaaacta actgcttctt ccgacgattg
    67921 aatggagaaa tgattgattc ttaataattc cagccatttt tcgttcattg gagaatcagc
    67981 cgatagttct ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca agtataacct
    68041 ttgccaatag atactttcgt gatctaagtc gcagcaccac tctaaatatt tttcgatccc
    68101 caaaaagaaa tgttgtttgt tttcgctaat gaattttaag atatcttgat ggactatgtg
    68161 agaactgctt ttgatcgtat acaaataagc atcttccaaa tcttgaaaat atttataaaa
    68221 agcaccgcgc gacatcccca tcttgtcaac gatttctgag actttgactt ggctgatgtg
    68281 ctgtgagcaa aatatctcca gtaataggtc atcgattctc tgtttctttg cgttcgataa
    68341 attgaaaaaa gtttcttttg gcatccacat ctctccttta tctctgatta tacgtggata
    68401 gcccttcatt gtcatgaagg attcattcct tctttttctc ttcttctgtc aagtacccat
    68461 cttccattcg ataaacgcga tcactccatt gaatcatacg gacatcatga gtaaccataa
    68521 tagtcgcttt ttgtttctca tgagcttctt taactagtaa tttgacgact tcatatgcat
    68581 gatctgtatc caaactagct gttggttcgt ctgctaagat caagcttggc tcattatata
    68641 aagcgcgagc aattgccact ctctgtcttt ctccaccaga aaggtcttta ggataactat
    68701 ttttaagtga ctggatatct aaagattgca gcaaggaatc gatttttgtt gaatcttctt
    68761 tttttgaagt aacttggtca actagttcaa attgttcctt taccgttaaa aaaggaatca
    68821 aattagagct ttggagaata aatcctattt ctttaaagcg taaatctgct cgtttttttt
    68881 ctggaagctc agttagtgag tgaccgttga tcactatttc tccggaagtc ggtgtttgca
    68941 atcctcctga aatggtaagg aaagtacttt tacctgaacc agaaggacca atgatgctga
    69001 cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa agctgagacc tttgtatgac
    69061 cagaaccaaa tgttttggta acttttttca tcgttaatat ttttttcatt tttattaacc
    69121 tcctattgct gtaattgggt ctactttggt tatcgtacgt atcgaaaaca atccgcctag
    69181 cattgcgaca atgatcaaaa tcccactata tagcaaccat tgtgaccaaa aaacagcaaa
    69241 aggcatcgcg cttggtaaaa tcagactagt caaataagca aacaaaagag caagcagaac
    69301 gcctaccacc ccaacaataa aggactgtgc tactaaagat ttagcgatga agaaattttt
    69361 gatcccttgt gctttcatta ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat
    69421 gaaaataccg accacggcag caaccactaa gaacaaaaag tatatcatcg catttaaggt
    69481 catattttgg gcagaatagc caggaatact ttcaatgaat gtcccaatag ttaataactg
    69541 tagacccttc gatgcttttt ctttcgaaat tttagctgaa tcctttatta cgatcccatt
    69601 gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt
    69661 atctagggac gcatagatga cgggagaaac tgtgtaataa gtttcaggaa agataccgac
    69721 aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta tctccaattt tatagcctga
    69781 atcagccaga ttttgagaga tgatgatttc attctttgtt ttaaattcat gccctttcgt
    69841 gagttttggt aataaaaatg cttctttcgt tgtgccgaaa actgtaacat tttctttgtt
    69901 ttttcctttg attgctccgc tgtacaatcc gattggtgct ttctctcctc cttcaatgta
    69961 tttcagatct cctcttgtta attgtgaagc tgcaaatact tgatttgcat cctccgacaa
    70021 aacaatctct tgtgcgtccc aatcatcgat tgctttttta aactcttctg aaagtccatt
    70081 cgctagtcct gacaacatga atacgacata cgcaatcaag aacataatac cgacgatcaa
    70141 gccatatcgt aatttagaat agcgaatttc tttgatagct agaaacattt gtttttcctc
    70201 cctatgatag attgatagtg acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac
    70261 aatttgtcac ttgtgttgtt tttttcttat caataaattg tctaagacat ctttcgataa
    70321 aaccttaaca tgagtcataa aaaatttatt taagccttat ttatgctttc catgataaac
    70381 taaagtgacc aaatcaatga ggtgggaaaa tgattaaact attagtagta gaagacgata
    70441 aagtattatc caataatata aaagaaatac tcgctgcaat cggcgagata acacaagttt
    70501 atgatggtag tgaaggactg ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg
    70561 atttgatgat gccggaaatg aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa
    70621 tacagactcc agtattgatt ttgactgcaa aagatagttt ggaagataag attgaaggat
    70681 ttgaacgagg tgcagatgat tatctaacaa aacctttcta tcgagaagaa ctgatcatgc
    70741 gcgtgaaagc attattgaaa cgctcgctag gtctctttga tgaaaatgtt ctgaagtata
    70801 aggatatcag ttgtgattta aaaagcaatg aagtcttgta taaagaacaa gttttgccca
    70861 tacaaggaaa agagtttgaa ttgctcgttt attttttaca aaataaggga atcattttga
    70921 caaaagagca gatatttgac cggatctggg gatttgattc tgatacgacg attacagtta
    70981 ttgaagttta tatgagcaac ttacgaaaac atctgcgaag tactggattg gataaagaaa
    71041 tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat gaatggagat
    71101 attacacgaa agcaacgtag tcgctttttt gcgacaaatg tagcagcatt tgcttttatc
    71161 ttttttcttt tgggattcat tactttacag atattaaacc aatctgcata cagagaaaca
    71221 gatctgtcat tagagaaaat aaattttgat tctcgaacag tacaattaga gatacaaaga
    71281 tatcagcaag gcgatccctt tttaaacccg actcaaacgg aacaaaacag aacaaacatg
    71341 gaacagccat taatggaacc atcaaatgac cgttttaaca ctcaagtgat cttgtggtct
    71401 aaaaatggtg agatattgaa taaagcagct attggtgggc gtttcaacca aatcaaagaa
    71461 ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata ttgaattaaa tgaagacagt
    71521 ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc
    71581 gttgcctaca tccaaatttt agaaaataca aatcaaatca agaattcatt agcgactttt
    71641 cgcacgatta tgattttgtg tatgattgtt ttttggattc tctctattgg tatcagttat
    71701 tacttgtcta gtctaagcat gcgccctatt attctttctt ggagaagaca aaaagaattt
    71761 gttgagaatg cttctcatga attgcgcaca ccgttaacga ttattcaaaa tagtttggaa
    71821 catcttttta ccaagcctaa tcatactatt attgaagaat ccgaaagcat cgctcaagca
    71881 ttgtcagaga cgaggcgcct cactggattg acttcggatt tgctgacaat cgctcggaat
    71941 gattctgatc agcagctgct ttcaaagcaa atgatcaata cacaagaata tatcgaacaa
    72001 cttgttaaac catttcaaga gatggccatc atagatggaa aagagtttat tctccataac
    72061 gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt ggttattttg
    72121 ctggataatg cattaaaata tacgaatcca gaagataaga tcattttgga ttcatctgtt
    72181 aaccataaag aatggctgat tcacgtaaag aatactggcc cttctatcag tgaagaagat
    72241 caagcaagga tttttgatcg attttatcgg gaagatcaat ctcgggcgaa agagactggc
    72301 ggatacgggc ttggcttggc aattgcaaaa caggtaatcg aacagcatca tggacagatt
    72361 tctgtatcca attatcttcc acaaggagtc gattttaaag tccaattgcc gttgaagcct
    72421 aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt
    72481 tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcttta
    72541 agctgaccac ttctgtcaca acctctttag cttacgctta cgatttttta ttgcggtctt
    72601 caatgtgtac atcaccgctt gtgtcaatat ccaattcttc tcggctaaca tcttctgtca
    72661 ctttttcaac atcttcatgt tcttctttat gaatcccaac ttcttcacga ataactgtat
    72721 gtttgcttac atctacttgc tcttctttga ttgggatagt aatcgtgtca tcttccaatt
    72781 tttcgtctgt tccctgtgaa gattgatctg ttactggttt gcgttcgatg acgatttctt
    72841 cacgtttgac cggtacttct accgtttcag tatcgttgac cacatgtttg tggatgtcga
    72901 cttctccagt tgtcacatca tgtgtattta catctaattt ttcttctttt agttgaattg
    72961 tatcatcatc tagatttggt ttgcgatcga tgtcttcagt cttgcttgta cccattgtat
    73021 cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt catattcaat ggatccgttg
    73081 tattttgaaa gcctgtcgtg ccacctacgt ccattggatc tgttccagtg ccagtttcta
    73141 atccagtccc cattgtgctt gctgggctaa cagtatcttg tgtatccatt gtatcttctg
    73201 ttgtttctgg ctgacgatag ccttttactg tgatgacaaa ttttccatca gaaatatctc
    73261 ctctatattg atataaagga tcattttctg gtgtagccgt ctcagaatca taggtatcaa
    73321 aagaaaaagt atcttttatt ttttcccata aagaacggtc ttcttcacca tccatccgat
    73381 tttcatctgt ttcaacatct ataccagaca atccgtcaaa tcctagtcta gatgctgctt
    73441 catcagtagt ataaataacg atatcatctc gttcataccc ttcgttacgc aaccgttcaa
    73501 ttactgataa tgcttcttcc ctcgtactat agcttccgat gaccgttaaa tttttctctt
    73561 ccatgtaaat tcctcctagc aaatagattt atgattggta tattgttact t
//
LOCUS       DACEMS010000003        61392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEMS010000003 DACEMS010000000
VERSION     DACEMS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.003"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..965
                     /locus_tag="IW735_000142"
     CDS             <1..965
                     /locus_tag="IW735_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1043174.1"
                     /translation="YGLAQDNRTAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEG
                     GKVAYLAQTPGVVTSKDSTEEGKINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDIS
                     LKEEYGLAVGDTVKLSGSDKTFKLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSE
                     NARINAIVVRGKISNLPEDLEQIKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIG
                     IFIYVLTMQKINIFGVMKAQGITGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLP
                     DAVPFQSNWLFFGVISLLMLVVAVLGALFSVRTIVKIDPLKAIG"
     gene            980..1648
                     /locus_tag="IW735_000143"
     CDS             980..1648
                     /locus_tag="IW735_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043175.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1668..2246
                     /locus_tag="IW735_000144"
     CDS             1668..2246
                     /locus_tag="IW735_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1043176.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2649..3560
                     /locus_tag="IW735_000145"
     CDS             2649..3560
                     /locus_tag="IW735_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1043177.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(3816..4733)
                     /locus_tag="IW735_000146"
     CDS             complement(3816..4733)
                     /locus_tag="IW735_000146"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1043178.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            4935..5372
                     /gene="zapA"
                     /locus_tag="IW735_000147"
     CDS             4935..5372
                     /gene="zapA"
                     /locus_tag="IW735_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1043179.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5430..5978
                     /locus_tag="IW735_000148"
     CDS             5430..5978
                     /locus_tag="IW735_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1043180.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6093..8453
                     /locus_tag="IW735_000149"
     CDS             6093..8453
                     /locus_tag="IW735_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1043181.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8540..8854
                     /gene="trxA"
                     /locus_tag="IW735_000150"
     CDS             8540..8854
                     /gene="trxA"
                     /locus_tag="IW735_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1043182.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9036..10814
                     /gene="uvrC"
                     /locus_tag="IW735_000151"
     CDS             9036..10814
                     /gene="uvrC"
                     /locus_tag="IW735_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1043183.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            10959..11858
                     /locus_tag="IW735_000152"
     CDS             10959..11858
                     /locus_tag="IW735_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043184.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            11851..13113
                     /locus_tag="IW735_000153"
     CDS             11851..13113
                     /locus_tag="IW735_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1043185.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13275..15173
                     /locus_tag="IW735_000154"
     CDS             13275..15173
                     /locus_tag="IW735_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1043186.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(15472..16191)
                     /locus_tag="IW735_000155"
     CDS             complement(15472..16191)
                     /locus_tag="IW735_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1043187.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            16528..16860
                     /locus_tag="IW735_000156"
     CDS             16528..16860
                     /locus_tag="IW735_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043188.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            16847..18850
                     /locus_tag="IW735_000157"
     CDS             16847..18850
                     /locus_tag="IW735_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1043189.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            18869..19339
                     /locus_tag="IW735_000158"
     CDS             18869..19339
                     /locus_tag="IW735_000158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1043190.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19360..19944
                     /locus_tag="IW735_000159"
     CDS             19360..19944
                     /locus_tag="IW735_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1043191.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            19959..20945
                     /locus_tag="IW735_000160"
     CDS             19959..20945
                     /locus_tag="IW735_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1043192.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            20935..21246
                     /locus_tag="IW735_000161"
     CDS             20935..21246
                     /locus_tag="IW735_000161"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1043193.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21268..23049
                     /locus_tag="IW735_000162"
     CDS             21268..23049
                     /locus_tag="IW735_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1043194.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23042..24418
                     /locus_tag="IW735_000163"
     CDS             23042..24418
                     /locus_tag="IW735_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1043195.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24425..25060
                     /locus_tag="IW735_000164"
     CDS             24425..25060
                     /locus_tag="IW735_000164"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1043196.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25090..26445
                     /locus_tag="IW735_000165"
     CDS             25090..26445
                     /locus_tag="IW735_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1043197.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26637..28031)
                     /locus_tag="IW735_000166"
     CDS             complement(26637..28031)
                     /locus_tag="IW735_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1043198.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28123..29094
                     /locus_tag="IW735_000167"
     CDS             28123..29094
                     /locus_tag="IW735_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1043199.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29091..29873
                     /locus_tag="IW735_000168"
     CDS             29091..29873
                     /locus_tag="IW735_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1043200.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30042..30413)
                     /locus_tag="IW735_000169"
     CDS             complement(30042..30413)
                     /locus_tag="IW735_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1043201.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30547..30990
                     /locus_tag="IW735_000170"
     CDS             30547..30990
                     /locus_tag="IW735_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1043202.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31033..31842)
                     /gene="yidA"
                     /locus_tag="IW735_000171"
     CDS             complement(31033..31842)
                     /gene="yidA"
                     /locus_tag="IW735_000171"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1043203.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(31861..33228)
                     /locus_tag="IW735_000172"
     CDS             complement(31861..33228)
                     /locus_tag="IW735_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1043204.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33212..34042)
                     /locus_tag="IW735_000173"
     CDS             complement(33212..34042)
                     /locus_tag="IW735_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1043205.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34205..34642
                     /locus_tag="IW735_000174"
     CDS             34205..34642
                     /locus_tag="IW735_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1043206.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            34718..35338
                     /locus_tag="IW735_000175"
     CDS             34718..35338
                     /locus_tag="IW735_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1043207.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            35527..36033
                     /locus_tag="IW735_000176"
     CDS             35527..36033
                     /locus_tag="IW735_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1043208.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            36050..36949
                     /locus_tag="IW735_000177"
     CDS             36050..36949
                     /locus_tag="IW735_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1043209.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            37036..37920
                     /locus_tag="IW735_000178"
     CDS             37036..37920
                     /locus_tag="IW735_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1043210.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(38112..38183)
                     /locus_tag="IW735_000179"
     tRNA            complement(38112..38183)
                     /locus_tag="IW735_000179"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38148..38150),aa:Arg,seq:ccg)
     gene            38243..39577
                     /gene="rpoN"
                     /locus_tag="IW735_000180"
     CDS             38243..39577
                     /gene="rpoN"
                     /locus_tag="IW735_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1043211.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            39780..40817
                     /locus_tag="IW735_000181"
     CDS             39780..40817
                     /locus_tag="IW735_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1043212.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            40858..41859
                     /gene="gap"
                     /locus_tag="IW735_000182"
     CDS             40858..41859
                     /gene="gap"
                     /locus_tag="IW735_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1043213.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            41969..43159
                     /locus_tag="IW735_000183"
     CDS             41969..43159
                     /locus_tag="IW735_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1043214.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            43263..44018
                     /locus_tag="IW735_000184"
     CDS             43263..44018
                     /locus_tag="IW735_000184"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1043215.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            44134..45432
                     /gene="eno"
                     /locus_tag="IW735_000185"
     CDS             44134..45432
                     /gene="eno"
                     /locus_tag="IW735_000185"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1043216.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            45832..46971
                     /locus_tag="IW735_000186"
     CDS             45832..46971
                     /locus_tag="IW735_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1043217.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(47038..47613)
                     /locus_tag="IW735_000187"
     CDS             complement(47038..47613)
                     /locus_tag="IW735_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1043218.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(47618..48592)
                     /locus_tag="IW735_000188"
     CDS             complement(47618..48592)
                     /locus_tag="IW735_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1043219.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(48616..48660)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            48875..49843
                     /locus_tag="IW735_000189"
     CDS             48875..49843
                     /locus_tag="IW735_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1043220.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            49956..50234
                     /locus_tag="IW735_000190"
     CDS             49956..50234
                     /locus_tag="IW735_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1043221.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            50352..50588
                     /gene="secG"
                     /locus_tag="IW735_000191"
     CDS             50352..50588
                     /gene="secG"
                     /locus_tag="IW735_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1043222.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            50700..51446
                     /locus_tag="IW735_000192"
     CDS             50700..51446
                     /locus_tag="IW735_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1043223.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            51449..53806
                     /gene="rnr"
                     /locus_tag="IW735_000193"
     CDS             51449..53806
                     /gene="rnr"
                     /locus_tag="IW735_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1043224.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            53828..54292
                     /gene="smpB"
                     /locus_tag="IW735_000194"
     CDS             53828..54292
                     /gene="smpB"
                     /locus_tag="IW735_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1043225.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            54594..55313
                     /gene="atpB"
                     /locus_tag="IW735_000195"
     CDS             54594..55313
                     /gene="atpB"
                     /locus_tag="IW735_000195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1043226.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            55366..55581
                     /gene="atpE"
                     /locus_tag="IW735_000196"
     CDS             55366..55581
                     /gene="atpE"
                     /locus_tag="IW735_000196"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1043227.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            55672..56196
                     /gene="atpF"
                     /locus_tag="IW735_000197"
     CDS             55672..56196
                     /gene="atpF"
                     /locus_tag="IW735_000197"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1043228.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            56183..56725
                     /locus_tag="IW735_000198"
     CDS             56183..56725
                     /locus_tag="IW735_000198"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1043229.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            56748..58304
                     /locus_tag="IW735_000199"
     CDS             56748..58304
                     /locus_tag="IW735_000199"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1043230.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            58318..59220
                     /locus_tag="IW735_000200"
     CDS             58318..59220
                     /locus_tag="IW735_000200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1043231.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            59243..60649
                     /gene="atpD"
                     /locus_tag="IW735_000201"
     CDS             59243..60649
                     /gene="atpD"
                     /locus_tag="IW735_000201"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1043232.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            60663..61085
                     /locus_tag="IW735_000202"
     CDS             60663..61085
                     /locus_tag="IW735_000202"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1043233.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        20379 a        10748 c        13135 g        17130 t
ORIGIN      
        1 cttatggctt agctcaagat aatcgaacag cagtcgacaa gtgggaggca gattcgattg
       61 tgctttcgaa agatgcaaat tcaaatctag ggatgtccat gattactaag aagatagcag
      121 aagaagtcga aggtgggaaa gtggcgtatc ttgcccagac acctggagtc gtgactagta
      181 aagacagtac agaagaagga aaaatcaatg ttagcttctt cggtatcgat aaaaatcaat
      241 tcatcatgcc caatcttgtt gaaggaaaag cttttgataa cgatgatgag gcagtaggcg
      301 atattagttt gaaagaagaa tatggtctag ccgtaggaga tacagttaag ttatctggta
      361 gtgacaagac gtttaaacta actggcttta cggatcatgc caaattcaat gtctcaccag
      421 ttctctatac aaccatcaat gcttaccaag agatccgctt cgaaaaagaa gatacaagtg
      481 aaaatgcacg gattaacgca atagttgtca gaggtaaaat cagtaattta cctgaagatt
      541 tagaacagat caaaatttct aaattcatca atgaattacc tggatataat gcgcaagtat
      601 tgacttttgg atttatgatc ggatttttga tcgttattgc ggctattgtg atcgggattt
      661 tcatttatgt actgacgatg caaaagatta atatctttgg tgtaatgaaa gcacaaggga
      721 tcacaggagg atttattgcg cgttctgtag tggcacaaac attcatttta tcatttgttg
      781 gtattcttct tggtctatta ggaacagtgg gaacatccct tgttctacct gatgcggttc
      841 cattccaaag caattggctg ttttttggcg tcattagctt gttgatgctg gtagtagctg
      901 tgttaggcgc actattttct gttcggacaa tcgtaaaaat cgatccgttg aaggcaattg
      961 gataagaagg aggaagagca tgaaagcaat agaatttaaa gaagtagaaa aaagtttcca
     1021 agatggcgac caagtagtta aagcattgaa agaaacaacc atgagtatcg ataaagggga
     1081 atttgttgca atcatcggtc cttctggttc aggtaaaagt acttttttga ctttggcagg
     1141 cggattgcag acgcctagca gtggctctgt atttatcaat gatcaagaat tcagtaatca
     1201 aaaagaaagt gaacgcgtta aactacgttt taaagaaatt ggctttattt tgcaagcttc
     1261 caatctggtt ccttttctga cagtaaaaaa gcagttatta ttagtagata agatcaaaca
     1321 tgaacatcgt caaaaagaag caaaagaatt atttgagcag cttggcgtgg ataagttgat
     1381 caacaagtat ccagaagaac tatctggtgg agaacggcaa agggtcgcaa tcgctcgtgc
     1441 attgtacaat gatccgtcca ttattctagc agatgaacca actgctagtt tggattcaga
     1501 aaaggcacga gaagtagtcg atatcttggc aaaagaatca aaagacaaaa ataaagcaac
     1561 gatcatggtt actcacgata cacggctgat tgacaaatgc gatagaatat ttatgatgga
     1621 agatggtgta ttgaaagaaa aagaataacc aaaaagcagg tggaaaaatg gtccatcgat
     1681 acaacaaaga tacacagcaa aaaattttga aagaagcgac tgacttgttt atgtccaagg
     1741 gatatctcgg cacttcaaca agagagatcg cacaaaaagt tgggatcacc caacccaatc
     1801 tctatcatta tttcggtgat aaagaaaaac tatatacggc agttttagaa aaccacttaa
     1861 aagatgttgg aaaagctttg agagaaatcg ttcaaacaag cgaaacggga tttcaagtaa
     1921 ccttgacaaa aatggcgcag tttttgattg agacacatct ggtcgatttg tttctgatgc
     1981 ttcacgatct tgaaagcaat ctttcaaagg aaacaagaga tcatcttttc tttctatgga
     2041 agaaaaatta tcgtgaacct tttgaagaaa tcttttcagc aaaccaatct gtgctaagaa
     2101 atgggatttc aaaagaaatc gcagcaaggc attttttctt agtgttagca ccttacatca
     2161 ctcggtcgaa aagacctgtg gaagaaccgc taaatgtggc gcaattgatc gatttatatt
     2221 tgaatggtgt attaggagaa gcgtaaagct gttatatagg gagaaaaata gttaggtgaa
     2281 gaagcaagat atgacgaaga tcgttatatc ttgttttttt tatataggta gtttttcata
     2341 aatacaaatt tgttattctc tcatgattat cagggtatat ttttatttat tatgtcggtg
     2401 ttccaggaag tgttttaatt taataacgaa gaaattaatt gtttgtagaa tgaaggaaac
     2461 tatttattgg gtagatgaat caccacgaat agtcaaaagg agatttcagg atatgccaaa
     2521 aatgaaaaaa atcattttag cagtaatgtt attttctacg gttatatctt ttacagcccc
     2581 ttttttgcgt acttaagctg attttaagtt ttgaccctta gtctggatct acgaggtgag
     2641 agtcaagaat gaaaaaaata attggagctt ttgtatttgc aatcattatc attattatct
     2701 gttttcaaat agatgttttt ggtcaaaaaa agatagttgg acaaacggtc agcagtacga
     2761 acagtacggt tacaaagaca aaggaatcat ttgatgatac agaacaagag acattaatca
     2821 aaatcggaaa aaatgaccgt acactatata cagatgactc gctaactacc ccacttgcaa
     2881 ctataaacgg cggagagctg gcggagtttc tttctgaaac aaaaaatgct tatcagatca
     2941 agacaaatga tggctatact ggatatttag ccctcgaaga tggaaccaaa gtcacaaaga
     3001 atatacaaac aaaaccggaa gagctatctg atgcagtgat tgtattagat cctgggcatg
     3061 gcggaaatga tactggggca ttaagcaatg atgaacaaac agaagaaaaa gatataacat
     3121 taagtacagc cataaaagta aaaaaggcgc tagaggatgc tggagcaacg gtttatttga
     3181 cccatactac agatgaatta gtacagcttg gagatatatg cgactacagt gaagaaaaaa
     3241 aggcagatgt ctttatcagt ttacacgcag actctacgga atatgcgaat gaagcaactg
     3301 gaatcacgac atattattac tatggacaag aggaaacatt agcacagaca attgctgact
     3361 ccttcactga tctgcctctg gattcaagag gaatctccac tgggaactat caagttcttc
     3421 gagaaaatct tcaaccttct attttaatcg agatgggtta tatgaataat gatagtgatt
     3481 taaaggaatt agtaacagac agttatcagc agcagattgc tgcaagtttg acacaagcat
     3541 tgaccactta ttttcagtaa taaataagcc cagccaaaca aaaatagctc ctcatatttt
     3601 tcgtttggcc gggcttatta tcgcagatgc aagcttttga acaccgttta aggaagaggt
     3661 tgtgagcctg ccgtttaact ccgcgtcaca aggagcaatt tcaaaaaaca gcttctcatg
     3721 tttgatgact attgtgactt gtgaccgagg agttggcatg agaacaccgt ttattcggaa
     3781 caagggactg tgacatgact tttgtcacag ttccattatt tttttagcag cttcaaagct
     3841 ttttctgtat tggcaaaatg aagtttggca tatttttcaa gcataggcat gccgcctttc
     3901 tccaatagtt ttgccgctac ttggtcagat ttcgttccag ctcctgatgg aagggtgatt
     3961 ccagcttcag ctgatgcttt gtgtaactct tccaaaaagc tggcacgact gatgatagaa
     4021 gcagcagcaa cagctaaatg atattgttct cctttagtaa cgaagtacag tttttcttgt
     4081 aattgatttt tttctaaacg cgcatatttt ttaaaatttg cttccggcgt aaactgatcg
     4141 atcaaaatcg cttctggttt ttctggctcg atttttttta gcagcaaatg gatcgcttga
     4201 ttgtgcaaag cgactttcat ccgaacggca ttgtattttg gttggatctc attgtatttt
     4261 tctggagata agacaagcag tttatagggg atggtttttt ttaagatgtc agcaagctgg
     4321 atgatttggc tatcttttaa ctcttttgaa tctctaacac caagtttacg taattctgca
     4381 agctgatcac gtttcacata agcagcacat actgttagcg gaccaaaata gctgccattt
     4441 ccgacctcat cactacctaa aacggataat tgactgatat tttttggcaa agaagaggct
     4501 gtttttgctg gcttggcttg tttatcctgt gcctcccccc atttatcagc ttctattgct
     4561 gcattcgtac cttgaaacat cacttttccg gatgtataag cggtaatggt cgtcccgtct
     4621 ttttttgcag caaagctagt ataaggaatc tgtttattta acaaataaga gcgatagtat
     4681 gctttcattt gactaagttg gtcgtttgtt gctttgataa tgacgttgct cataaaattc
     4741 tcctcgtttt tatacataaa actataccgc aactcacgac ttttcgtttt ctaagagatt
     4801 gcgagacata gcattttttt aaagtttatc atataatagt taagtatgtg aaaaatattt
     4861 gtggagaacg gtgaaagaac caacttcatg gtacactaaa taaaactttc atcaaatagg
     4921 aggaaatgat tcgtatggcg cacgaaaaaa cacgctacaa agcagtgatt gccaaccaaa
     4981 catatactat tatcggacgg gaaaccaagc atcatatgga tatcgtaacg aagctgatca
     5041 atgaacagtt agcagaattg aaacaattat caccacaaat ggataatgaa caagcagcaa
     5101 tcttgatggc tgtcaatgcg ctttcggatc aattgaagaa acaagaaaga atcttggagc
     5161 tggaagaaga aacagcagaa ttaaagaaaa aaatgatcaa atttacagag cttgaaaatc
     5221 gtgtgaaacg cattgaagca atcgagaacg aagctcggga agtattgaaa gaaaatggtc
     5281 aagcagatca taccattaaa aatcatgtgg aagcacaaca gattttaaac gagaaacgaa
     5341 aaggacaaat taaacaaaaa tcttctaatt aagatccttt tatcaaaata agaaaaattt
     5401 aaaaaagtta gaagagaaag gaacgtagca tgctgagttt gctgatttta tttttattgc
     5461 taattgcgtt tttttctgga gcacgtcgcg ggtttgcttt gcaaatcata ttcgcgattg
     5521 gatatgtatt gtcatttatt gctgcccagc atttttacaa accattagca agtcatttgg
     5581 aattatacat tccataccca gctgtcaccc caacatcaaa gttagcattt tttgatcagg
     5641 tatttgcttt tcacctagat gaagcttttt atgcaggaac tgcttttctg attattttgc
     5701 taattggctg gctgcttacc cgatttgtgg gggtttttgt ccatggattg acgtatgtgc
     5761 cgatcttgag acaggtagac tggattgcag gaggcatcct gtcactaatc atggcttacg
     5821 ttactatatt tttgattttg cagttacttg cctttgttcc attagatgtg gtccaaaatc
     5881 aattcggcga gcatagtttc gctcgtttta tcgttgagag aacgccattt cttaccaaca
     5941 agatgtatga gctttgggta acgaatattt tgaattaagg aaaatagtgc tggaaaatat
     6001 ttcgtttttt gtacagagta gtttatttaa gaaggctgcg tagcgtatgt ccggcacagc
     6061 tcttttttca tagtgaaaga taaggtgata aaatgaataa ccgaatcctt gaaacattgg
     6121 aatttgaaaa agtcaaacag atggtcagac aatttgtagt gactgcacaa ggaaaagaag
     6181 aactggcaga attggtacca gtgagtgaga aacaaacgat cacaaactgg cttcaagaaa
     6241 ctgaggatgg attgaaagtg caacgtctgc gaggcgggat ccctatacct aaacttgaaa
     6301 atatccgtcc ccatatgaaa cggatcgaga tcggagcaga cttgaatgga gtggagcttg
     6361 cacaagtttc cagagtattg tctacgacaa gcgaattgaa tcgtttcatt gatgatcttt
     6421 ctgacagtga aatcgaattt gcacgtttgt atatgtgggc agatcagtta gtcacgatcc
     6481 ctgttttgag ccgccgttta aaggaagcaa tcgatgaaga cggacgcgtg actgatgacg
     6541 cttctcctga gttaaagagc ataagacaga acattcggcg aagtgaacaa gctgttcgtg
     6601 aacagttaga tgggatcgtc cgtgggaaaa atgcaaagta tttaagtgat gcgatcatca
     6661 ccatgcgaaa tgaccgctac gttatcccag tcaaacaaga ataccgcggt gtatttggcg
     6721 gtgtagtcca cgatcaaagt gcttcaggac agactttatt tattgaacca aaacaagtgg
     6781 tggatttgaa taatcgtttg cgtcaatacc aaatcgctga acgaaatgag attcaacgta
     6841 ttttaagtga actttctgct gaacttgttc cccatcgtca agaaatcatc cacaatgctt
     6901 atgtgattgg taaaatggat ctgatgaatg caaaagcacg atttggtaaa gaagtaaaag
     6961 ccatcgtgcc gggaatcagt gaagacaatc atgtcgtctt gaaacaagca agacatccac
     7021 tgatcgatca ggaaaaagta gtttctaatg acatcacaat cggaaaagac tatcaagcca
     7081 tcgtcattac aggacctaac acaggtggga aaacgatcac gttgaagact ttaggtctgt
     7141 tacaactgat ggggcaagca ggattaccga ttcctgcagg tgaagaaagt caaatcggta
     7201 tttttgagga agtatttgcg gatataggag acgaacagtc gattgagcaa agtctatcga
     7261 ccttttcttc tcatatgacg aacactgtag atattttatc aaaagtcaat gaaaaaagtc
     7321 tggtattatt tgacgagctc ggtgcaggaa cagatccgca agaaggtgct gctctagcaa
     7381 tcgcaatctt agatgatctt ggtaaaaagt ctgcttatgt gatggcaaca acgcattatc
     7441 ctgaattgaa agtttatgga tacaatcggg caaatacgat taatgccagc atggagttcg
     7501 atgtggatac actaagtcca acgtatcgtt tgctgattgg cgttcctgga cgaagcaatg
     7561 cgtttgaaat ctctagccga ttaggtcttg atacagaagt tattgatgaa gcgaagcagt
     7621 tgatgaatga tgagagccaa gatttaaacg aaatgatcac agatttagag aatcgccgga
     7681 aaatggcaga gaccgaatat ctggaaatgc gtcattttgt ttctgaggca caagaacttc
     7741 acgatgattt gaaagaagct tacagctatt tctttgaaga acgtgaaaaa gaaatggaaa
     7801 aagcaaagaa aaaagcaaac gaagtcgtgt cagaggcgga agaaaaagca gaaaaaatca
     7861 ttgctgatat tcgtaaaatg caacagcaga ttgggcaagg aaatgtgaaa gagcatcagt
     7921 tgatcgatgc aaagacccaa ttagcaaatc tacatcaaga agaaactttg aagaaaaata
     7981 aagtattgaa gaaggcgaaa gaacaaaaaa cgttgaaacc gggagacgaa gtgttggtta
     8041 cgacttacgg ccaaagaggg acgcttttac gtaaaaacgg gaatcagtgg caagtagaaa
     8101 tcggtatatt aaaaatgaat gtgtccgaag acgagctgac gcctgttgct cctcaaaaag
     8161 agccgacaca acgagttatc catgctgtac gatctgaatc tagtagtcac gtgccgaatc
     8221 aattggacct tcgaggaaaa cgatacgaag aagcgctaag tgaggtagat caatatttgg
     8281 attctgccat tctagctggt tatcctcaag tgactattgt ccacggaaaa ggaacaggcg
     8341 cacttcgcaa gggaattact gattatctta aaaaccatcg gagcgtcaaa agctttgagt
     8401 ttgcaccggc aaaccaaggc ggaaatggtg cgacaattgt taaatttaag taaagcttac
     8461 taaaggtaag cagatggttc gctatccaag tatagaaatg ctatactaat catagatttt
     8521 aaaaatggag gtctttgata tgtcacaagt aattacagat aagacattta atgaagaaac
     8581 agataaagga cttgtattga ttgatttttg ggcaacttgg tgtggtccat gtcgtatgca
     8641 agcaccgatt ttggatcagt tggaacaaga atatgatgaa gaagaattca gaattgctaa
     8701 aatggatgtg gatgaaaatc cagaaactcc tcaacaattc ggtatcatga gcattccaac
     8761 attaatgttg aaaaaagatg gtcaagtagt agaaaaagct gttggtgttc attcaaaaga
     8821 acaactacgt caaatgatcg atcaatattt ataaaaactt acagcaaaaa taagctgagg
     8881 gtgggactga atccagttcc gtcctcggtt tttttgcggt tgagttaagg ttattcaaat
     8941 taatattttt tgagtataat tacgttgtca ggctggattg tttttttgac agacatgcta
     9001 taataacttt aattgaatat gaaggggagt accagatgaa cgaacgaatc aaaaacaaat
     9061 tagctttact tcctgatcag cccggctgtt acttgatgaa agataaaaat ggaacgatca
     9121 tttatgtcgg caaagccaaa atattaaaaa atcgtgtccg ctcatatttt actggcagcc
     9181 ataatacgaa aacggaacgg cttgttagtg aaatcgaaga ctttgaatac atcgttacag
     9241 aatcgaatat cgaagcattg ctcttagaga tcaatctgat aaagaaaaat gatcctaaat
     9301 acaacattat gttaaaggat gataaaacct atcctttcct taagataacg aatgaaaaat
     9361 atccccgttt ggtgattact cgtaaagtac taaaagacaa agcactgtat tttggaccat
     9421 atcccgatgt cggtgcggcg aatgagacaa agaagatcct cgatcgatta tttcctctga
     9481 gaaaatgcaa acctagtcag acgaaggagc cttgtcttta ctatcatttg ggacaatgtc
     9541 tgtgtcctta ttatttcgac gtagatccga aagtctatac gaaaatcgtt gatgaagtga
     9601 aaaagttttt aaacggagga cacgaaaaaa tcgagtcaga gatccatcag aaaatggaaa
     9661 aagctgctga aaacatggag tttgaaaaag ctgcagaata ccgagaccag atccgtgcca
     9721 ttgagacaat catgactcgc caaaaaatga caaatactga tttactggat cgagatgtat
     9781 ttggttatgc gattgacaaa ggatggatgt gtgtccaagt gtttttcgta agacaaggaa
     9841 agctaattga acgagatgtt tcaatgtttc ctttctataa cgaagcagaa gaagatttct
     9901 tgacttacct cggtcagttt tatcaggaaa atgagcattt cattcctaaa gaagtcttga
     9961 ttcctgatac gatcgacaag cccagtgtag aagctttgtt agctacgaaa gtgctgcaac
    10021 caaaacgagg agaaaagaaa aaattagttg aattggcgaa taagaatgcc tcagttgctt
    10081 tgaatgaacg gtttgatctg attgccagaa aacaggaacg gacgatcgga gcagttgaac
    10141 gtttaggaaa cgcgatgaat atcccgaccc caatacgtat cgagtctttt gataactcta
    10201 atatcatggg gacgaatcct gtggctgcga tggtcgtatt cattgatgga aaaccggcaa
    10261 gaaaagaata tcggaaatat aaaataaaaa cagtcaacgg accggatgat tatgcatcaa
    10321 tgagagaagt catctatcgc agatattcac gagtgattcg cgaagggttg cctttacctg
    10381 atttgatctt gatcgatgga ggaaaaggac aagtagatgt ggcaaaagat gtcttagaaa
    10441 atcaattagg aatcgatatt cctattgctg gcatggcaaa aaacgataaa cataaaacaa
    10501 atgagttgct attcggtata gatttggctg tagtaccgct tgagcgaaat tcaccagagt
    10561 ttttcttact ccagcgaatc caagatgaag tccatcgatt cgcaatcact ttccatcggc
    10621 agctacgcag taaaaacagt tttgcttccc gattagatga aattgaaggg ttaggaccaa
    10681 aacgaaaaaa aatgctgctt aaagagttca aatcgttgaa aaatatcaca gcagccacga
    10741 cagaagaatt acaagaaatc ggactgccga aaaatgtcgc acaaaacgta tttgataaat
    10801 tacatcagga atgaggaaag ggcactttac aaagaaagta aagtgtccta tcattttatg
    10861 tacaacaaaa agagcgttta cgttttgttg gtaaataata aatcgcttac gtctggtgtc
    10921 taaggatata tgtgatttct tgaagaagga gtgaagaaat gttagaagtc aaggatcttg
    10981 ttaaaacttt tgggaactac acagctgtcg atcatgtctc tttccaaatc cccgatggaa
    11041 aaatcatggg gctaattggc caaaacgggg ctgggaagac aacgactttt cgtttgatct
    11101 tggatttttt gacacaagac caaggagaag tgttatggaa tggccatcat cttggggaga
    11161 aagattatga tgtcatcggt tatcttccgg aggaacgggg actttatccg aaagtaacga
    11221 ttcaagacca actgctttat tttgcagaac ttagagggaa atcaagaaaa gagattgaac
    11281 caaaaatcga tttttggatg gaaaaatttc aggtaaaagg gaaaaagacg gataaagtaa
    11341 aatctttgtc taaaggaaat cagcaaaaag tccagctgat cgccacgtta atccatgaac
    11401 ctaaattgat cattttggat gagccattca gcgggttaga tccggtaaat gctgaattgc
    11461 taaaagatgg aatattggaa ttgaaaaaac atggttcatg tgtcattttt tctagccaca
    11521 atatggataa tgtagaaaaa atctgcgatc atttgatcat gttgcgaaac ggaaaaatgg
    11581 ttttgaatgg aaaagtacat gagattcgtg aatcatttgg ccgtacgaag ttattcttgg
    11641 aatctggttt aagccaacag gaagtagaag aaatcgaagg cgtgaaaaaa gtgatccttc
    11701 atgaagacaa tacgttagag attaccttag aagatcctga agctgggaaa gaaatcttta
    11761 ctcgtgcgac tcagttcggc tacattccta tgtttaacca gcaaccgcca actttggaag
    11821 agattttcaa attgaaagca ggtgaaatcg atgcgtaagt tttggattat taccaaagat
    11881 gtctatttaa aaaatgttaa gtcgatttct ttcttgatca tgatattagt cccttttatc
    11941 atgctgggta tcatttatgt agcaggaaac tttgctcaac aaaatagtga tgtcaataaa
    12001 atcggcgtat tggctgataa tgagcaaatc agtcagcaaa tgagccaagt caaaacagat
    12061 gattttgagt tcaaagcagt tgattctgaa gaaaaagcac aaaaagagct ttcggaagaa
    12121 aaaatcgatg cgtatttagt agtctcaact gagaatgata agataaatgg gatgctttat
    12181 agcgaaaatt cattaggaca aagtacacaa ctactgatac aacaacaact gactggcttg
    12241 caatcgatgc ttcgagcaag tcaattaggc atttcaccag aagaagtagc tagcttgagc
    12301 caacaagcaa gtttgactcg acaaaaagtc aattttgatt caaaaggaaa aatgacagtt
    12361 ggagaagata actcgacagt ccaatatgtc gtaagttatg tagcaacgat tgtcttgttc
    12421 attattattt tgacgtatgc gcagatcatt gcacaagaga tcgcttctga aaaaggcacg
    12481 cggatcatgg aagtgatcct gtccagtacg actgcacaaa agcattttta cggaaaacta
    12541 acaggtgttt tacttgttgc attaacgcaa atggcattat acggtattat ttttggtata
    12601 ggatacagtc aattcaaaga tatggaaatg gtcaaaaata tattagacgg tatctcattg
    12661 gataacttga ttggaccatt cttgtggtat tctcttctat tcatgatctt tggcattttg
    12721 attttttctg ttttagcagc tttatgtggc tctttggtca acaaagcaga agatacagca
    12781 aaagcaattt tgccagtgac ttatctatct ctaggcggat atatgcttgg attgatacta
    12841 ggagcttcag atccaaacaa tatcgttatt cggatcactt cgtatattcc attcttatct
    12901 tcatatatta tgccggttcg cttagcaaat gagacagtcg gtatgggtgg agcattgatt
    12961 tccttgttgg tacttgtagc cgtaacattc ttattgatga tcttgtctgc aaacatgtac
    13021 aaatcgaatg tactcgtgta tagcgaaggc ggattatgga gttcattgaa acaatcgatt
    13081 tcaattatga gaaatgaacg aaaaaaagca taaaatatag aaaaatatag aaggaagctg
    13141 gacatttatt gttcccagct tcttttttgt atagacctat ttctattttt tattatattg
    13201 gatatatata ttcattgaaa atagagcata acaactttat aatgttgagt ataccaattt
    13261 ttggaggcgt tttaatggac cgctatttag atttactttc agagaagttc cctacaacgg
    13321 aagatgtcat cacggaaatc atcaatctcg aagcaatttt gcagcttcca aaaggaacag
    13381 agttattttt gagcgatata catggcgaat ttcctgcttt tgatcatatt ctgcgtatcg
    13441 gttcaggaaa tcttaaagaa aagatcaaag accttttctc tgaacaaatg acggaaacgg
    13501 agatcagcca attcactatt tttacggctt atccagaata tgtgttgacg actgattggt
    13561 acaaaaaaca agataaatcc cagctgatcc atcgtttgat cgatctttta cggtttacga
    13621 cagtcaaata cactcgatca aaagttcgaa aaggattgcc gaaagaatat agctatatca
    13681 ttgaggaact cttgtatatc gatgatcgta tcaacgggaa aaaagactat gtaaaaaaaa
    13741 tcattgaaca acttgtcttg atgaaggaag aagaaaaatt tttaacaaaa cttgcgcaga
    13801 cgatccaaaa atctgtcatc gatcatttgc atatcgtggg tgatattttt gatcgaggaa
    13861 ctcaagcagc caaagtaatg gatcgaatca tggatttttc ctctgtagat atccagtggg
    13921 ggaatcatga tattttgtgg attggtgcgt attgcggctc tgaggcatgt ttattgacgc
    13981 ttttacggat tgccgcacgc tacaactatt tatttgaatt agagaaagaa tatggattaa
    14041 atttacgtcc attattttct tttgcccatc aaacctatca gaagaatcca gtatttaccc
    14101 ctaaaaacag gaaagatcta tctgaaagag agcaagaaga attagaaaag atccatcaag
    14161 cactttctat cctccaattc aaatcagaac atcagttgtt gaatcgacga cctgaattta
    14221 aaatggacga tcgtaaattg ttggaaaaaa tcaactatga ggaatcaacg atcgttttag
    14281 aaggtcagct ctatgagttg aaagggactt gttttcaaac gatccagcca gatgatccta
    14341 aaaaattctt ggaagaggaa caaaaagtga tcgatagttt gatgtattca ttccagcact
    14401 cgttaagaat gcaaaaacac atgacgtttt tgatcgaaaa agggggaatg tatctcacga
    14461 acaatcagca tgtactgttc catggctgta tacctgtaga tgaaaagggg cagctcttag
    14521 catttgaatt agaccaatct tatcgcggaa aagaactgct gggatttttt gaaaaacaga
    14581 taactgaaag cgcaaaagat ttgactgcaa atgaagattt ggcgaccgat ttgatttggt
    14641 atgcgtggag cgggccattt tcgcctttat tcggcaagag taaaatggca acttttgagc
    14701 gctattttct cacagagcct catacgcacg tcgaaaaaag caatccctat tatcacttgc
    14761 gagatgaaga gtggttcagt gaaaaaattt tggcagaatt cgatgcaaaa gaagaaggtt
    14821 cagcaatcat caatggacac acgccagtaa aagtaaaaaa aggagagtca ccgatcaaag
    14881 caaacggaaa agcatttgtt attgacggag gtctatccaa agcttatcaa aaaacaaccg
    14941 ggattgctgg ctactcatta ctatgcaatt cttacggttt ccaaatcgtg acacattatc
    15001 cgttcttgcc tattgaacaa caatttgcaa aaaaatctga tcaaacgaat gtaaaaaaag
    15061 tgatcgagca gcagctccca cgcaaactta atcggaatac gacaaaagga atggaattac
    15121 agcaacagat tactcaactc caaaggctgt tggattatcg aaaggacgat tgagaaaaat
    15181 gctagccaac attgctcaat agactaaaaa gcgactgcaa caaaaggcgt gtcgcagtct
    15241 ccttcggaat tagctcaaaa aacggtaaaa tattagaagc tatttttctg tttatttctc
    15301 taatactcag gtcagggcgc tgatttcacg acctcttcct taaacggtgt tctcctgcca
    15361 actcctcggt cacaagtcac aatatccaac aaacatgaga agctgttttt tgaaattgct
    15421 ccttgtgact cagagttgaa cggcaggctc acgacctaat cagaaaaacg attaaaattc
    15481 attcacaaag aaatcgaata aacgctgttc agatgaatct acacgatgag aaagttccgg
    15541 atgccattgg acaccaagga ttttgtgcat attatcttta ctttggactg cttcaatgag
    15601 tccatcgttg gaaaaggcga tggcttttaa caaaggagaa agatctttga ctgcctgatg
    15661 atgataggaa ttcacaaagt atgaatcagg gagaaggtca cttaataaac tgtccgaaac
    15721 gattttgact tcgtgagttg caaactgcgg ggcagtgggc tgttgttcgt gttttacgga
    15781 ccattttgga tataatgaaa gatcttgata aagtgttccc tccaacacga cattcaatag
    15841 ttgaagtcct cggcagactg caaaaatcgg ttttctttgt ttcaatgctt ccaagatcaa
    15901 tgcctgttcg aattggtctc gttgaatatt tgtttccgtc agttttggat gagggtcttc
    15961 catgtaaagt tgcggactaa catcctgacc gcctgcaaga atgagtttgt cgattttctc
    16021 aattagttga ggcgctgaat ctggagacat gactggcaga ataagaggta atccttgagc
    16081 atctaaaact gcatccacaa atccctgcgg tgtatagctt acttgatttc cttggaaagt
    16141 atctgtcgct cgaatcagtt gattcccggc aatgccgatg attggtttca tttttattcc
    16201 ccctaaagta gactaattgt aacaaggatt taaaaaaatt taaactatgt aagacttgcc
    16261 cctatactta tctgttcttg ctgtgttact atctgtaaag agaattttcg tgtgaaattc
    16321 atgacgatag ttgaaaatat tgcttaattt gttataataa aaagtaagca gaaataaaaa
    16381 ggttgggcgt aagcttcaag gatctagttc ttcaaggggt gagaaggaca ttttattgaa
    16441 ggggcctgcc tatactaagt tatctttaga ggtacatact ttcaatgaaa aaagaaagca
    16501 tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc
    16561 cgaaaagcag aagaagaaaa tgagaagcgt gctcgaaccc tacggcagca gttgaagacc
    16621 tatgcagaag aaaaagagca agaattggag ataatccaaa cgaacagtcg gcaacagctg
    16681 aaacagttgg tagaagaaaa agaacaattc gaaaagcaag aattagaaaa agaaaaaatg
    16741 aaattgaaag cagaagcaga caaggtgcaa gcaactttga aagaccaata tcaaaagcat
    16801 cagagtcaag caatcggtgc aatcattgaa agggtgaaag aaacttatgg ccgtcactaa
    16861 gatggaaaaa gtgaccctca tttcagataa aagaaatcag gagaggattt tacaagcagt
    16921 acaaggaatc caaaatgttg aaatccggga tctgtatcaa aatagaacaa acaaccagtg
    16981 ggtagaaaaa tattttccag ataccacgat tattgataaa gaagcaaaat taagcgcttt
    17041 aggcaatcgg ctgaaagaca tccaagaagc tatccaattc atacaacacc acggcgatag
    17101 ccgtcaaaag aaactccatt tgaaacatca agaactttcc ttacaagact tggaagcaaa
    17161 ttattccgaa gaaaatttca tgaataaatt agaagaagtc cttgatttaa aagaaaagtg
    17221 ggaacagcta actgaaaaac gtgatcgttt agaagaagag gaagaatggc ttcttcattg
    17281 gcaacagctg gatattgctc caagtatgta tgcaagtaag acgaccattt ttgagatggg
    17341 gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa ctaagtaagc ttgatgacgt
    17401 ttatatggaa gaaatagatt atacgccaaa acaagtttat ctttcgtatg ttgctttaaa
    17461 aaaatcagtt gctgctatag aagaaattgc tgggaaatac gggttcaata aagaaacgta
    17521 tccttatgaa caaaatccta aagaaaaatt gcaagaaacc aaacagcaat tatccattgt
    17581 tcacgagcag caaaaaaaga taacgacagc gttaggtcag tgcagcggct atatccaaga
    17641 atttgaatgg gtagaagaag tcacacttgc cgttgcagaa cgggaaaaag taaaagaacg
    17701 gtttatccat gccacttatc tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca
    17761 gaatttgatg catgtgttaa acgaaactgt atcagaagaa gatatatacg ttagtttcga
    17821 aacaccaaac tcagaagaga ttcagcagga agtccctact aaattgaaaa accatccgct
    17881 tatcgaacct tttgaattgc tgactgaaat gtacagtctg ccaaaatatg aagaagtaga
    17941 tccaacgcct tggatgacac cattttatct tgtcttcttc gggatgatgg tcgcagatgt
    18001 tggttacgga ttgctgatgc tcattgggac aatactagct caaaaactat ttgttttacc
    18061 ccgcgggatg aaacgtttcg taaaattctt tgagattctc tcggtaccat cgatcatctg
    18121 gggattgatc tacagttcat ttttcggaca ggcattacca aaagaactat ttggtatccg
    18181 cttgcctttc ccaatcctat ctacgacgga tgacgtcaat acgattttga ttttatccgt
    18241 gattttcggt ctgatacaga tcttggttgg attattcgtg gcagcaaagg aaaatatcag
    18301 gaggaaagat tacttgagtg ctgtcagtga tggttttgcc tggcaaggga tcctgattgg
    18361 gatcgttatt gcattgattg gtgtcatgtt gataaaaaat acagcatttg tttatcttgg
    18421 cgcggggcta tcgatcattg ctgctttatg tatcttaatc gtgccgatta tccaatcgcc
    18481 gtctaaagtc aagggtgctg ccaaaggagc gtataatctt tacggattga ctggttatat
    18541 cggtgattta gtcagttata cacgtttgat ggcactaggt atttcaggcg gaagtattgg
    18601 cgcggcattc aatatgctag ttgctttcat gccgccagcc gctcgattca gtgtagggat
    18661 ccttttgatc atcgctttac acgcactgaa tatgtttttg acactgttaa gtgcctatgt
    18721 ccatggtgca cgattgcagt atgtcgaatt ttttggaaaa ttttatacag gaggcggacg
    18781 tgcctttgac ccgctgaaaa cggcggaaaa atacgtcaac atcaatcata agaaaaaaaa
    18841 caataaataa ttggaggatt tttatacaat gatggattac ttaattagtc aaaatggtgg
    18901 aatggtattt gcagtattag cgatggcaac agcgacgatt ttttcaggga ttggttcagc
    18961 taaaggtgtt ggaatgacag gtgaggcagc agcagcgtta acaacaagtc aacccgaaaa
    19021 attcggacag gctttgattc tgcaattgct tccaggtacg caagggttgt atggattcgt
    19081 tatcgccttt ttgattttta tcaacttagg aaatgatatg tcagttgtac aaggattgaa
    19141 ctttctaggc gcttctctgc cgattgcttt tacaggtttg ttttcaggta tcgcccaagg
    19201 gaaagttgca gcagcaggta tccaaatttt agcgaaaaaa cctgaacatg ctacaaaagg
    19261 aatcattttt gctgcgatgg ttgaaacata tgcgatttta ggtttcgtta tttccttcct
    19321 attagtattg aatgcctaag cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc
    19381 atctctcaga tgaatgaagc agcagagcaa gaaagagcgg cgttagagca agaagagcga
    19441 atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaaa tcgaaacaga acatcaaaaa
    19501 caaaaagaaa aacaaatcga attacttgaa aaaaaatacc gtcagcttag gaatcgccaa
    19561 caagtagaag tacgacaaga aaatttgaat gcgaagcagg aatttttacg acgattgttt
    19621 gcagatgcaa tagctgaaat ggagaattgg gatgaatctg aacaaatcca gtttatcaaa
    19681 aattcattgt attcattacc tttgacaggc aaagtaacat tcattgctgg agaaaaatca
    19741 gcagcttatc tttctcagac attgctggat gaatggaaca atgaacttcc ctttatgatg
    19801 gttttaagtg atgaaactgt tgctgatcaa gcgggttttc tgataaacga tcaaggagtt
    19861 cagtataatt ttcttttcag cagccttgta caagatatcc aaggaacgat gagttttgag
    19921 attgccaatc aattattcga ataaggaggg atcagtgtat ggaataccac gaactaaatc
    19981 ccttgattcg cggaagagag cttgaacttt tgtccaatga gacatttgaa cggatgattc
    20041 aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc cacgatttac agtccatata
    20101 tttatgacgg atatgaatat gacttcgaac agaatctttt aaaggagcag agccgtctat
    20161 ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt ttggatctat acgatgcgct
    20221 atactttcca taatctgaag gttttgacaa aagccgagat gacaaatcaa aatctggacc
    20281 atttgtatat cgacgatgga ttttattcat tagaaacctt gaaggatgcc attcatacgc
    20341 aagtttcaaa tgaactccct gaaagcatta tggacagtat acgagaagta cgtgattatt
    20401 ttgaagagtc taccattctt cagggaattg acgtgattta tgatcgacag tttttgacag
    20461 agcagcgacg gcttgccgat aagctaggat atcaagaatt gctgacagaa gtcattgctt
    20521 ttattgactt gaccaatatc acaacgatgg ctcgaggaat attgcagaaa agaacatatg
    20581 ggttcatgac gacggtactt tccagctcgg gaagtatcga aaaagaagta tttttgaact
    20641 ttgtagataa agatatggaa gattacacga attttctatt agagacagat tatgctgatt
    20701 ttctgcgccc tgtcatccgt gagggaaatc ttgatctcgt aaagcttgaa cagttgaaag
    20761 atgattatct aagttcattg tatcaagaag cacaaaccca ggctttcgga ccactgccac
    20821 tactagcctt tttgaacgca aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg
    20881 gcaaaagaag ccatttcgaa ccagaaacaa tcagaaagcg ggtgagacaa gtctatgacg
    20941 cataagatcg gtgtagttgg cgacaaagat tctgttttgc cattcaaatt gtttggcttt
    21001 gatgttcgtt atggtacaac caagcaaacc gttcgtcagg caattgaaga aatggcaaag
    21061 aatgagtatg gcgtgattta tgtcacagag caatgtgcag cattgagccc agaaacgatc
    21121 gaacgttaca aggaacaaat gatacctgct atcgtattga ttcccaatca tcaaggatca
    21181 ttagggatag ggctggaaga aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc
    21241 ttatagtatg aaaaggagat agaaattttg caaattggaa aaatcataaa agtctccgga
    21301 cctcttgtaa tggctgagaa catgtctgat gccagtatcc aagacatgtg tctagtggga
    21361 gatctaggag ttatcggaga aattatcgag atgcgtggcg atgtggcgtc tattcaagta
    21421 tatgaagaga cttctggaat tggtccaggc gaaccggttc gctctactgg tgaagcttta
    21481 tctgttgaat taggaccagg gatcatttca caaatgtttg acggaatcca acggccatta
    21541 gataccttta tggaaatcac acaaagtaat ttcttagggc gcggagttca attgcccgca
    21601 ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag aagccggaga aacggtgagt
    21661 gctggagatg ttatcgggat cgtagaagaa acaaaagtta taaaacataa aatcatggtg
    21721 ccaaatggga taaaaggaac gatcaaaaca atcgaaaaag gttcattcac tattgatgaa
    21781 acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg
    21841 ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc cggatgcacc catgatcact
    21901 ggacagcgag tgattgatac attcttccca gtgacaaaag gaggagcagc agctgtacct
    21961 ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg
    22021 gacatggttg tctatgtggg atgtggcgag cgtggaaatg agatgacgga tgttttaaat
    22081 gaattccctg aattgattga ccctaataca ggagaatcgt tgatggaacg tacggtattg
    22141 attgctaata catcaaacat gccagtggca gctcgagaag cttctatcta tacgggaatc
    22201 acaattgcgg aatatttccg tgacatgggt tatgacgttg ctatcatggc agactccact
    22261 tcacgatggg cagaagcact tcgtgagatg agcggacgtt tggaagaaat gcctggtgat
    22321 gaaggttatc cagcttatct tggttcacgg cttgcggaat actatgaacg atctggaaga
    22381 gtcattgcat taggatcaga aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc
    22441 ccgtctggag gggatatttc ggaaccagtc acacaaaata cattgcgggt cgtaaaagta
    22501 ttctggggtc ttgattccag tttagcccaa aaaagacatt ttccttcgat cgattggata
    22561 caaagctatt ctctttattc aacagaagta gggaagtata tggatcatat ccttcaagct
    22621 gactgggcat ccatggtcac cgagggcatg cgtattttgc aggaagaaca gcagctgaat
    22681 gaaatcgtgc gtttggtagg gattgattcc ctttcagata atgaccgtct gacattagaa
    22741 gtggcaaaat ccattcgtga agattatttg cagcaaaatg cttttgatga tgtcgataca
    22801 ttcacttcaa gagagaaaca attcaaaatg ttgaagatga tcttgacctt tggtgaagaa
    22861 ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga tcatggacgg taccgtatcg
    22921 attcgtgaaa ggatcggacg aagcaaatat atcccggaaa cagaattaga aaaactagat
    22981 caaattattg gtgaaatcaa gcaaacgatg caaacgatcg tttcagaagg aggcatgagc
    23041 aatgattaaa gaatatcgta cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa
    23101 agttgctggt gtcaaatatg aagaattaat cgaagtccgt atgcaaaatg gcgagattcg
    23161 tcgtggacaa gtattggaag tccaagaaga taaagcaatg gtccaaatat tcgaaggaac
    23221 aagtggtatc aatttacgtg actcttctgt acgattttta ggccatcctt tagaactagg
    23281 tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg ggacgaccga aagataacgg
    23341 accagagatc ctgcctgaaa agcatttgga tattaacgga gaagtaatca atccagtagc
    23401 tcgggattat ccagatgagt ttatccaaac aggtatctct gcaatcgatc atttgaatac
    23461 attagttcgt gggcaaaaat taccagtatt ttctggctct ggtcttcctc ataaagaatt
    23521 agctgctcag atcgcgcgac aagcaacggt actcgattca tcagatgatt tcgccgtagt
    23581 ctttgctgct attggaatca cttttgaaga agctgaattt ttcatggaag attttcgtca
    23641 aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga
    23701 acgtatcgct actcctcgta tggctttgac tgctgcagaa tatcttgctt atgaaaaggg
    23761 aatgcatgtt ttggttatca tgacagacat gaccaactat gcagaggcat tgcgagagat
    23821 ttctgcagca cggcgagaag ttccaggaag aagaggatat cctgggtatc tttacacgaa
    23881 cttagctact ttattcgaac gagcaggaag aatccgcgga ctaaaaggat cggttactca
    23941 aattccgatt ctaacaatgc cagaagacga taaaactcat ccaatccctg acttgactgg
    24001 ttatattaca gaaggacaga tcatcttgac tagagagctt tacaagagtg ggattcagcc
    24061 accaattgat gtactcccgt ctctttcgcg gttaaaagat aagggaactg gacccggaaa
    24121 gacaagagaa gaccatgcag ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa
    24181 acaggcaaaa gaattagccg tagttttagg tgaatctgct ttatctgatg tagacaagat
    24241 ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa
    24301 ccgttcgatt actgaaacac ttgatctagg gtgggaactg ctatcgatgt tgccaagaac
    24361 cgaattaaaa cgaatcaaag acgacatgct ggacaaatat ctgccagaag ggaagtgagt
    24421 tctgatggca cgattgaatg tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca
    24481 gttgacaact gccacaagag ggcataaatt attaaaagat aagcaagatg aactgatgcg
    24541 tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt caagaaatgg aagagtctat
    24601 gacagaagca atgtctgatt ttgtattggc aaatgcttcg atcacggaac ctttcatcga
    24661 agaattattt gttttgccgg cggaaaacgt tgatttgtct atcgtagaca aaaatatcat
    24721 gagtgtaaaa gtacctgtga tgaattttga atatgatgaa cagctgacag acacaccttt
    24781 ggaatacggg tatttgaatt ccagtgccga actggatcgg tcaatcgatc ggtttactac
    24841 cctgcttcca aaacttttgg aactgacaga aatcgaaaaa acttgtcaac tgatggcgag
    24901 tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa tatatgacga tccctcaatt
    24961 agaagaaacg atctattata ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac
    25021 aaggttgatc aaagtaaaaa atatgggtac cactgactaa gtggtatctt tttttaaaag
    25081 aggaaaacga tgattaaaca aagcaaacaa ataaagaaac gtttgccggc tgttcagctt
    25141 ctcgcattag gattttttct cctgatttta gttggcggcg gcctacttac cctaccattt
    25201 tttagtaata gtgggcatgg gacaaatttt atcgatgcgc ttttcactgc aacttctgct
    25261 gtctgtgtaa cgggattgac aacattgaat acagcagagc attggaacag tgctggtcaa
    25321 tttttgatca tgcttttgat tgagatcggc ggtcttggtt ttatgatggt tccgattcta
    25381 tgctttgcat tgataaagaa gaaagtcagt ctcagcacac gtattatatt aaaagaagcg
    25441 ctgaatctag aagaagtatc aggtgtgatg aagctcatgc tttacatcct aaaattggcc
    25501 tttgttatcc aattgattgg tacggttgct ttatcttttg tatttgtgcc tgaatttggc
    25561 tggttgaaag gtatctggta cagtttgttc catgcaatct ctagtttttg taatgcaggg
    25621 tttgatttgt taggagacag tttagccaat caccagcaaa atgtttattt gatcatggtg
    25681 gtatctgctt tgatcatttc tggtgggtta ggcttcatcg tatggcgtga tttactggaa
    25741 tatcatcaga aaaagaagat gacgattcat tcaaaaatcg cattaaccgt tacaggtacc
    25801 cttttattag gcggattcgt tgtgttcttt ttcacagagc gcaatggggc aacgctagtg
    25861 gaagggaata tgtttcaacg attggcaaac acctttttta tgagtgtgac acctagaacg
    25921 gctggatatt attcagttga ttacctccat atgagccatg caggtctgat tttaacgatg
    25981 tttttgatgt acatcggcgg aacatctggg tcgacggccg gcggattgaa aacaactacg
    26041 ttaggtgtgt tgctgatcca gatgcgttcg atgttgaaag gacgtacgag agcggaagca
    26101 tttggccgta ccatccgaca aggcgcagtg ttgcgggcac ttactttatt ttttgttacg
    26161 ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga ctgaaacgat acctaaaact
    26221 tctggcatcg aatatattgc ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg
    26281 atgggattga cgccagactt gactgcaata gggaaattga tcattatagc gcttatgtac
    26341 attggacgag ttggtgtatt gactgtggtc ttctctctga tgacaaaagc acaaagaaca
    26401 caatcaaaat acaaatatcc agaagaaagt gtcatgattg gataatttca tcttctgaca
    26461 gaaacctagt gatttagtaa aataagaaaa taaaataatt tttcttgctt cttggatact
    26521 ttaagtgcaa aatgttcgtt tattttttga ggcactaccc gttgttgaac ctcgggtagt
    26581 gcctcttttt tattgaaagg aatcaataag atttgattcc tttatttatc aataggttat
    26641 aattcatatt tttttatttt ttcttttacg atacttagtc cagaagctaa aatttcaagg
    26701 ttattagagg atagggaaat tcgaacgaat gcatctggtt gcgcacgtgt agtaaagcgt
    26761 ctggaatgat aaagtcggat accgttagca agaaagtcat tctcaattat cttagggtca
    26821 atctcaggat caactggaag aattcgatag taagggacac gattcgttgg tttttctact
    26881 ttgaaaagag aatcaaaaag ctgattggct ctttttgtaa gacttaattt agtttctaag
    26941 atcctatttg cttcatttga atggaggatt tgagtgatga cttccacatc taatccagaa
    27001 gttttcacat ttatattaaa aattgcttgt tctatcttgc tttggacatg tactggaact
    27061 acaagataag caactcgcaa tccagaacaa ataaattttg tcattccagc taagtagatt
    27121 gtttggttag gtaataaatt ttggaaagga ggaatgatcg tatgttgata gtaagttgtt
    27181 aaaaatgaat ggatatcatc ttcaataacc catagacctt ctttttcaat cacttcggct
    27241 aattgctctc gtcttttgtg agagatttga aaccctatag gattattaca agaaggcata
    27301 agaaagacac catgaatctt cttttttcta cattcctgaa tcaatatatc aggtcgcatt
    27361 ccttgctcgt caaaatcaat tggaacgatc tctaaatgat ataactgagc caattcaatg
    27421 aaattagcgt atgtatatcg gtctgttgct attctatcac ctggagagaa aagtgcagaa
    27481 agtgtgatag ccaatccatt ttgaacaccg gaaacaatag atagagattc taaggacgga
    27541 atgactcctt gagtttttaa ccaatttgct gcagtagtca attgaaaatt tgtgcctttg
    27601 ggatcttggt aattaagcaa accaacaaga tttgggttat tactagttga ttgaataaac
    27661 gggacaacga atttattaca ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt
    27721 tcggttactt cattggtaga gatggtgatg gactcaaaga cattgggaga attaaatgtg
    27781 ccacttccaa tattcgtata cagcaaacct ttctcaattc catatttata agccttgcca
    27841 acggtagtaa aatttaaatc taaataatca gctagctctc tttgggaggg taactgagtg
    27901 tttttctgta atttattctt taaaatatct tgttctaatt gttgaatcaa cgatcgataa
    27961 taaggacgct tcaatttttg tttgtctggt cgccaagtta acgggtaatt gtcataagaa
    28021 ttaataggca ttttctccac tccttaaaat tgtataccat acaattttac cattgattgt
    28081 attgatttta gaagttaatc ttttgtgagg agggaattgt ctatgaaaat tattaatttt
    28141 gaggaagtag tagcttcttt tagttttgaa gaagcgattc aagtaatgaa aaaatgtttt
    28201 tttgattatg aaagtggtaa aatcagccaa aatcctcgat tggtggaaat tttacctgac
    28261 ggaaaaggca aaaatatgtt tgcgatgatg cctgcttatt tgggggagaa cagatatttt
    28321 ggcacaaaag tcattaccgc ttttccggat aatcatcagt atcatttacc ttctcattta
    28381 ggagagattt tcttgtttga tgctcaaaac ggactaccca aagcattgat aaacgcaaat
    28441 gcagtcacct ggattcgaac agctgctgta tctgcgctgg caacagatta cttagcaaat
    28501 ccgcatgcag aaacacttgc tttaattgga gctggtcaac aagcattgtc gcatttgact
    28561 gcgatttcta ccattcgtcc catcactaca gtgtttgttt acgatatttt agaagaaaga
    28621 cgaaatactt ttatcgctaa agctaaaaaa cagtatccta aaataacgtt tataaatgca
    28681 atatcagtag aagatgcagt acgtccagcg gaaatcattt gtacgttgac ctctagtaaa
    28741 gaggcatttc ttcaaaaaga gtgggtaagg gaagacgcac atatcaatgc tgtgggcaca
    28801 tttactcctg atacaagaga gataagtaca tcgttgatgg aaacttctca aatctatgta
    28861 gatgattact cagctgcctt aacagaaagc ggggacctgc ttatagccat ggcagaagga
    28921 gatttagagg aatcttgtat tgttggtaca ttaggcgaat tagtttctga gaaaaaaaca
    28981 ttttataaag gagaatcgag gccgacgatt ttcgacgcag ttggtctagc tattgaagat
    29041 ctttgttgtg cagaatatat ttataacaaa ttagagaaag gggctttaaa atgaacttag
    29101 attattacgt agttgatgca tttgcagatg aggtgttcaa aggaaatcca gcagcggttt
    29161 atgtgctaga agaatggctg ccagaaggta cgatgcaaaa aattgccatt gaaaacaatt
    29221 tatctgaaac agcatttaca gtgaagaaaa accaagaatt tgagcttaga tggtttacgc
    29281 cagatcgaga aatcgatctt tgtggtcacg cgacattagc aacagcattt gttttattta
    29341 actattacaa aatacctgat gaaacgatta aattttctac tcaaagcggt aatctatttg
    29401 tgacaagaat taaagattat tattacatgg attttcctag tattatgccg aaaaaagtgc
    29461 cgatattgtc tgaatatgaa gaagcgattg gggctaagat taaagaagcc tacttagcac
    29521 gtgatttatt tttcgtatta gaagatgaaa aaacggtggc taaattgcag ccagatttta
    29581 cagctatcaa aaattttgaa ttaggaatag gagtcatcgt gacagcagaa agtgtgcaaa
    29641 aagactttgt ttctagaacc ttctttccaa aattaacaat caatgaagat ccagtatgtg
    29701 gatcagcaca ttctaatctt attccttatt gggcagaaag aatgaataaa acaaaattgt
    29761 cagcttatca aatgtcatca aggggcgggg atctagaatg tgaattaaaa gaagatcgtg
    29821 tgataatagg aggaaaagca gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt
    29881 caaatgaatt aaaaataaat tggtggagag tggcaacctt atagctacaa tggttgttaa
    29941 aaaaagacgc aaactttttc aatttaatga aaagtttgcg tctcatttta attattggtg
    30001 tttgtttttt attcaatcaa gagtaaaatg ccccatacta actatcctcg gatgacttcg
    30061 attttataac catcagggtc aatgacaaag tagtaagaag gcgcagtgcc aggtaatcct
    30121 tttaagtcag taatctcaag accggcagct ttatgttgct cgtgtaattt ttctagatca
    30181 tcagctgcta tagcgatatg accatagccg tctcctagat catacgcacc atgatcatag
    30241 ttgtaagtca actctaattc atagtcatcc ccaggaagtg tcaaataaac aagtgtaaat
    30301 ttgttttctg gaaaatcacg gcggcggctt tcttcaaaac caaatgcctt ttgataaaat
    30361 tcgattgatg cttcaagatc tttcactcga acacatgtgt gagccatttt catagattca
    30421 tctctccttt tccttttact caatcataac aaaaagaagt aaggaaatag ttgatttgtg
    30481 cttgttcatg agaaatttct catgtaaaat ggatagaaaa taaaactgtt aaggtggaaa
    30541 ctattcatgg ataaaccaat ttttggtgaa aaaaagcaat ttgctgatta tcaaacccgt
    30601 tatgcagcat atattgtcat agaaagagat cagcagattg cagtaatcga ggctccaaat
    30661 ggtgcttttt ttcttcctgg cggggaaatt gaaggagaag agacgaaaga agaagcgatt
    30721 gtccgtgagc tgatagaaga aatgggaatt gtggcagaaa tttcttctta tctgggagaa
    30781 gctgatgagt atttttactc gaattatcgt gcaacttatt attataatcc aggatatttc
    30841 tttgttgctg attcttggaa gcgtatagga gaaccaactg aaaagaccaa taaaatttgg
    30901 tgggtgagcc ctcaagaagc gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc
    30961 gagagatgga tcgaaaaaaa taaaaactga aagaaagcga gtagatcagc tttctctcag
    31021 tttttttatt tattacagta catatttttt gattgcttca gctacaccgt gctcgtcgtt
    31081 tgatgaagtg atgacatttg ctgcttcttt gacaagagaa acggcatttc ccattgctac
    31141 tcctagacca gcatattcaa tcatggataa gtcgttctcg ttatctccga tagccatgat
    31201 ttcatcttga tcaataccaa ggtgctgtgc gagattcgca actgccgcac ctttacttgc
    31261 ttctttattt agtacttcga agtaataggg cgcacttttg accgttgtgt atttatcacg
    31321 gaaagtttgc ggtaaacgcg caattgctgc atctaaaatt tctggttcgt cgatcatcat
    31381 cattttgacg atgttcatgt ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat
    31441 cttgactaaa cttgcttcgt ggatcgtata agcgctgata tctcgattag gcgtgtagat
    31501 cgctttgtca tcgatcgaat ggagatgact gccgacacgg cgtgccatca cttcgatctc
    31561 taggaaatct tcatgtgtca gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg
    31621 tacgagtgcc ccgttataag taataacgta atcattatct tgaaaaaggt tcaactcgtt
    31681 taactgttcc tggacacctg gtaagggacg gccagtgcat aaaacgatcg aaacaccttt
    31741 tgcaacagca gcggcaatcg cttcttttac ttcttttgtt acttctcttt tctcatttaa
    31801 taaagtacca tcgatgtcga tagcgactag tttgatagac ataatttcct ccaatatatt
    31861 ttaatttatt tcaatcaatg cgccattacg gatatggctg gcaaattctt gataagtttc
    31921 gtcaaataaa tcataatgat cacgcaagga cggatcaacc atttcttttg gaaagaaaaa
    31981 tcgctcatct ccttgttctt gtcccgctaa tgcagcaaca agctgactaa cctgtgataa
    32041 ttctatcaaa gttccgtcat ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc
    32101 tgggcgataa aaatcataag gtagatcgta gctagaattc acagcagtgt aataaacagg
    32161 attatatccg accttttcta ctaacaaagt gagttcttgg actaattttg aatcccggtt
    32221 gccagaaaaa gtagcagatt tcagtggttt acgattcaaa aaacgtttag ctaaatcatt
    32281 taaaatagga tcagcagatt cagaccattg agtaaagtaa gtgcccaaga ccccatcatc
    32341 aagtttgagg tattcttcta aagtaaagct tccgtcaaga aatggcagaa gtaattgaga
    32401 atggagttca aaactgctgg catcttcttg atacaactcg tgtgcgcgat gaagcagatg
    32461 ctccaaaatt acttccatac cacgggaaac aggatggaag tacacttgca catacatttg
    32521 atatcggcta acgatataat cttccactgc atgcatgcca ctcatggaaa aagcgatccc
    32581 gccttcataa ggacgaatca cacgtaatat tcgggtaaga tcaaatgttc catattctgt
    32641 tccagtgaaa taagcatccc gaagcaaata gtccattcga tctgcatcga tctgactaga
    32701 aatcatttgg acaacttgtg ggttggggta agttttttgg atcacactag ctaccttttc
    32761 aggaaaacct tcttctactc ggttaagaat ctgatacact tctgtttctg gagaagtgat
    32821 gatctctaca gtgatggctt catgattcgt atggaagata tgttcaaatg tatgagagta
    32881 aggaccatga ccgacatcat gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc
    32941 atcccagcct ccgttgccta ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat
    33001 tcgtcttgaa atctcataaa cgcctaatga atgagtaaaa cggctatgtt ctgctccgtg
    33061 aaaagtgaaa gaagaagtgc ctaattgttt aatgcgtcgt agacgttgga cttctttcga
    33121 attgatcaaa tctaagatta cttgatgctg gacatggata tagttatgga ctggatcacg
    33181 aaaaactttt tccataggca gcatttgata tttataaggg gttgtcataa tgtagctcct
    33241 ttatgttatt acgttgttcc attctctgga tttggttgtt ccattgctcc agctggattt
    33301 ctttttttag ccagtcggta gaatcctgta aatcacttgc agaatacagc ttttcaaaag
    33361 cttttgcaaa tgcttctttg acttctttta caaaaagggg ttggtcaagc agttcttcaa
    33421 gtgttgccat tgtagcagga tccactgcag gatagccatt tgtaccaaag tcttctttta
    33481 aagcagattg ataaaatcgc ttcatgactt gcccgcgttt ttcctgatta ccgttgacac
    33541 ttaagtacat cataacagaa atcccatttt tgatccgacg ctgagcgata ccggcaaatt
    33601 ttttgccatg gatgcttaaa tcataagttc caggacaata agaatgggtg atttcgaatg
    33661 cttgtatcgg tgcctttgga aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt
    33721 catctatggt tatttttttt gtgtcacttt gggggaaaat caaagaaata ttcaaaattc
    33781 cttcatctga tacgatccct aatccacctg aatttctgat cactggagcg taacctgcat
    33841 ctttcagtgt ttggatcccg tcacttagaa aaggagttcg aacatccttc atacctaaga
    33901 tgactgtgca gtcatattgc cagaaatgta aaaagatttg ctggtgttct ttagaatatt
    33961 ccgttaatat atctgttaat gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat
    34021 ctagtcgaat cgcagggttc attcaaccgg ctcctttgta taaattcata ttttctacaa
    34081 tattatactc taaaatcaaa gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa
    34141 gttggttgac aaaacaaaag aaactaggaa caatgtagct atgagaagta aaggagaatg
    34201 aataatggaa ttatcacaag gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca
    34261 gcaaggggaa gtgcaagatt tttattttga tttgaaaggg caaatggtga caattggcga
    34321 tacgctgtac atacgttata aagagattca ggaggagacg ggcgatgaga ttcctgtaac
    34381 gatcaaatta gttccagatg gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg
    34441 tttgaaattt ggctataaag aacggttaga aacatcctat cgaacaccat acggtatgat
    34501 ccagattgct acttttacga aagaactgca tgttagtttg aaagaccgtc ccacaggagg
    34561 gaaggttcga atcgattatg atctgttcat gggaccggaa aaaatcggcg aatattattt
    34621 gaccttggat tttacagcgt aaatataaaa gaaaagtttg attttttgtg tctgattttg
    34681 tatgataagg agtagactgt gaaaggacgt gtcctgattg gaaattagtg tatttgaagg
    34741 tgcaaacaaa agcgaattat caatgatcga agtagcacac gcgatcttag aacaacgtgg
    34801 cgatgtcatg gatttttctg atttagctaa ccagatccaa aactatcttg ggaaatcaga
    34861 cagtgaaatt cgtgattcac tcgcacaatt ttatactgac ttgaatattg acggcagctt
    34921 tatctcatta ggggataatc gttggggctt gcgttcatgg tatgctatcg attcgatcga
    34981 tgaagaagta aaccatggaa tggacgaaga ggatgaagat actccacgtc gccgtaaacg
    35041 caagaaagtc aacgccttta tcaatgatga tgaagacgcg atcgattaca atgacgatga
    35101 cccagaagat acagatctga cagaagaaga tgacgatgat ctgtttgatg acgatgatga
    35161 tgaagatgaa gaaatcgctg cttataattc tgacttgcaa gaaatcggag cagatgaccc
    35221 gacagatgat gaagaagact tgccaggaaa tatcgaagaa gatttaagta tcatagacga
    35281 agacgatgat gatgaagatt atgaagagga agaattttct gactctgaaa aagaataaac
    35341 attattttct tacgagaggt tgtgacagag acgatcagcc tatcagaata aggaggtgtg
    35401 acacgatttt tgtcacacct tcttttttgt atataaaaat cccaagtaaa tacataaaaa
    35461 ataaaaaaat tgaaactttt agcgcacctt gtacgtcata ttaagtgagg cagaaaggag
    35521 tggagaatga tttttactgg atataagaaa aaacggcaga tcgaaaaata ccgtcacctg
    35581 ctgaatggac tcatcgaaac tgattatcaa aaaatgttcc gtatcacgat gaattacgta
    35641 catgataaag aagaggcctt agatgtgatg caagatagtt ttcataaagc cctgaactat
    35701 tttgaaaaag aaaaagagat cgagcatttt agtgcatggt tttatcggat attgatccgc
    35761 acggcattag atagttggcg cagacaaaaa aggacgcctg tggacttatt tgaaattcaa
    35821 gatatccccc aacagttacc tttagaaact tccgttgcag agctccatat gattttagat
    35881 aaaatagaca gtccggaaaa agagattctg atcctctact tttttgaagg atttcgtttg
    35941 aaagaaattg cgactatctt ggatatgaat gaaaacacag ttaaaacgaa aatgtaccgc
    36001 tcattgaatg ctttacgtca aatcctagaa tgaaggagag aagagtgcca tgtattccca
    36061 aaaagaaaat ttaaaaaaac taaagaaaac ttataaagaa caagcaattc ccaaagggct
    36121 taaagaggag atccgccagc gttttattat cgaagagcaa tcatttataa aagcaaagcg
    36181 gagaaagaga cgtctccaag ggttcggagt atctttggct atcgtaatga ttacaacaag
    36241 tagccttctt tttaatagtc aagttcgaag ctttgcagaa gatttaccca tcttaggatc
    36301 agtcattgaa ttgatcttag gcgaacgctt tactgatcga tctgaaaaaa tcgatattca
    36361 ggtaccgaag atcaatacac aaaatgagaa agaaaataaa acaattcatg gattaaatca
    36421 aaaatacttt cgcgaaggac aagcagattt tgaaaaagct gaaaaagaat atggaaattt
    36481 tgaaacggat cattaccaag ttttgggaga ttatcagaaa atcgtagacg ataaccggtt
    36541 tctcgtgatt gaaaggcaga tcacccaaac agcagcagat agccatgttg aaaaaagata
    36601 cgacacaatc gacaagaaaa acagtgtcca gctctcactt ccacttttat tcaaagatga
    36661 cacctatcta tctgttttga caaaagaagt caaacggcag atggccgaac aagtcaagga
    36721 agatccatct aagtactatt ggactgagca agatattcaa gaagggacga tagaaaaacc
    36781 aacacttgtt acgcctacac gaagttttta tttgaacaaa gagcatcaat tagttttgac
    36841 tttttctcaa tacgaaattg ctcctggtta tatgggtaca ccagaatttg tcattcctaa
    36901 gtccgtgaca aaaacgattt tggcatcgga agattatttg gatcattaga cgagaagtag
    36961 ttgcgactag cagtttcaat tttcaagtaa gtacaagtaa ataataagaa gagaaaaaga
    37021 gaaagaagag atatagtgaa aagaaattat ttagttatca gtattattgc tttatccgtg
    37081 cttttgtttt tcgaaattac ttcggtggcc tccttgatca ccgaagtaaa aggacaaaca
    37141 cactcttcgg agcattcgtc acaatctttc aaacaatcag aacaatcaac cattaaaagc
    37201 caatcaactg aaacagctaa cgtcaaagaa gccattcaag aatcagcgga aaaggtacaa
    37261 agtcagtcat cacaatcaac acaagacagt acaaaaacga gccgagatcc ggctgtcaaa
    37321 agcgtcgcaa tcagctttga tgatggacca ggagcgacca caacaccgca gcttctgcga
    37381 atcttaaaag aaaaaaacgt gcatgccact tttttcgtct taggagaaaa tacggcgcag
    37441 catcctgaaa tcgtgaagca gacagctgaa gcagggcacg agattggcaa tcacacatac
    37501 gatcatcaga atctggccac tctttctgcc cagtcgatga cggaggaagt gacgaaagca
    37561 gacactgaga tcaaaaaagt cactggcaaa acccccactt ttgttcgtcc gccctatggt
    37621 tctgtcacga atgtaggagc aacgatcatc cagcgaccga tcatcgaatg gtctgttgat
    37681 tcagaagatt ggaagacaag aaatcctgat ttgattttgc agaagatcca agcaacagta
    37741 tacgatgggg caattatttt attccatgat atctatcctg aaacgattcg tgccgttcct
    37801 caagtgatcg attatctgaa agagcaaggg tatcgcatca cgacagttgg ggatttatta
    37861 gggcatccaa cggctgtcga gaactattat ggcagaaacg atcatcggcc agttcagtaa
    37921 gtgagacaag tatctttaat aaataacaca ttttaataag tagttgaaca cataactacc
    37981 ttacttgcaa aggcttaaca atatctatac aggtacaaca aaaaactgtc caccaatttt
    38041 ctttggcgga cagttttcac ttaaaaataa agaagcaact attcgtaaat tatgctaaaa
    38101 tagcagtaga acgcgtcctg agggaatcga acccccgtct caagaaccgg aatcttacgt
    38161 gatatccact acactaagga cgcaagcact gggataattg taactaaaca gataaaaaat
    38221 tacaagggga ttggacaaaa aaatgaaatt tgagcaaaat ttttcacaaa aacagcagca
    38281 gacacagaaa ctagccatga cgcaacagtt gcaacagtcc atccaagttt tacaatataa
    38341 cagtgaggag ctgttggcat tcgttgagaa tcaagcaatg gaaaatccat tggtggaagt
    38401 tgttgagccg gaatggcagc cagattacat aaaagcttct tcatcttctt atgaaggaga
    38461 agagacaaat tacctaaatc agattcctga tacaaaaggg tctttatttg attcattgat
    38521 cgaacaagtc catttgaatt accgagatac ttttttgagg aaaatcgttc tttatcttgt
    38581 agaatacatc gatttgaacg gatttttgac gattagttta gaagaagccg gaaaagagat
    38641 tggagctacg cctatccaaa tgctcgatgc gttgactctg attcagcaat tagaaccagc
    38701 tggagtaggt gcacgtagtt tgcaagaatg tctcatgctt caaacagaac gagatgatta
    38761 tgcgccggaa ttagcttata tcgtgttgga agaatgtttt gaggaactgg tggagcgaaa
    38821 atggaaagaa atcgcacaac gtttttctgt tgatctacat gctgttcagc agatattcga
    38881 ctatttacaa acgttgagtc caagccctgg ccggattttc gaccgtagca gcgaattgta
    38941 cattattcct gatgtccggg tattagtgga cgacgataaa aatgtacaag ttatctcaaa
    39001 ccgaaaaaat cagccaaata tacgctttca agagagttac ttcaaacaaa tgtcacaaca
    39061 agcagataaa gaaactgaaa attatttaaa agaacggaaa caggaattcg aatggttgaa
    39121 gaaaacgatc ctacaacgtg gggatacaat tttgcgggtt gctcaagtca ttgtttcccg
    39181 gcaaaaagct ttctttatcg acaaggaacg tccaatcaag cctcttacgt taaaggaagt
    39241 tgcaactgag atagatgtgc atgaatccac ggtcagtcgt gcagtcaacg ggaaatattt
    39301 ggaaacagac ttcggtgtat ttgaattaaa aaaatttttc actacacgaa ttcctacgaa
    39361 tagtacagaa cagacagaag acctttcagc agatacagcg aagaaaaagc tgcaggaatt
    39421 agtcgatcaa gaagacaaaa acaagccgtt gtctgaccag aaattggttg aattgctgaa
    39481 aaaagatgag atagcgatct caagacgaac agtagcaaaa tatcgtgatt tattaggaat
    39541 cccgagttca tcaaaaagaa aacgttatga taactaatca gtcaaaagac tagaaaaagt
    39601 atttcacttt ttctagtctt tttagttgaa aaacgagagc aaaactgtta gacttacagt
    39661 cgtgaggttg agatttccct ttattgatgt gaaattcgaa tagttaattt ctttttttat
    39721 cgtatatggg tcgttttaag tctacattgg acgtaaagtg tccaactaag gagatcgcta
    39781 tgttggatga aataaaaatg attgaatctg ttgcaccaga tattatcgaa gtgttgcaag
    39841 aacgctataa aattttacga aatatctatt ggatgcagcc gattggacgt cgaagtttat
    39901 cggaaagtat ggggatcaca gaacgagtat tgcgaacaga aacagatctt ttgaaaaatc
    39961 tgaatttgat cgacacctca aaaagcggta tgacgctgac aactaagggc gaagaagttt
    40021 atcaaagcct tgaaaacttc atggatcagc ttttaggtac gcatcaaacc gaacaacagt
    40081 tggcagaata ttttggtaac caacgctgta tcgttgtttc aggaaacagc gaagagcaga
    40141 ccaaagtggc tgatgcattc ggcgaagcgc tgagcgaagc cctagaccga ctacttccag
    40201 aaggcgagaa tatcatcgct gtgatgggag gaactactat ggctgtggta gccgaacaat
    40261 tgtctaatct ggaaaataag aaacggcata acctgtttgt ccctgcaaga ggcgggattg
    40321 gtgaagcgat aactgtacag gcaaattctg tcagtgcaag aatggctgct aaagcaaacg
    40381 gtaaccaccg tgcactttat gttccggaac aattaagtct ggcaacttac aattctttac
    40441 tcaatgagcc atctatccaa gaagtcttaa acttgattag tgaagcgaat tgtgttattc
    40501 atagtattgg gcgtgcatta cacatggcag cacgtcgtaa gatgactgaa aaagaattag
    40561 tcatgctgaa acaagcaaat gctgttgcgg aatcttttgg ctactttttc aatgaaaaag
    40621 gtgaagtcgt ttataaggtc cctagaatcg gcatagaatt gcaagatttg gaaaaagtcc
    40681 cgatcatcat ggcaatcgcg ggcggaaaat caaaagccaa agcaattcga gcttatatga
    40741 aaaatgcacc aaaacaaacg tggctcatca ctgatgaagc tgctgcaaat gagattttaa
    40801 aaggggtaac cctttaaaat aaatattttt ttgattttca taaggaggaa atcttaaatg
    40861 acagttaaag taggtattaa tggttttgga cgtattggac gtctagcatt ccgtcgtatc
    40921 caagatgtag atggaatcga agtagtagca atcaacgact tgacagatgc taaaatgttg
    40981 gcacacttgt taaaatacga cacaactcaa ggacgtttca acggaacagt tgaagttcat
    41041 gaaggttcat tcaacgtaaa cggaaaagaa gttaaagtat tagctaaccg caacccagaa
    41101 gaattaccat ggggcgaact aggcgtagat atcgttctag aatgtactgg tttcttcact
    41161 tctaaatcag cagctgaaaa acatttaaca gctggtgcta aacgcgttgt tatctctgct
    41221 cctggcggaa acgatgttcc aacaatcgtt tataacacaa accacgaaac attaactggt
    41281 aaagaaacag ttatctcagg tgcttcatgt actactaact gtttagctcc tatggctaaa
    41341 acattgaacg acaaatttgg tgttgttgaa ggattaatga caactatcca cgcttacaca
    41401 ggtgaccaaa tgactctaga cggacctcat cctaaaggtg acttccgccg tgcacgcgct
    41461 gctgctgcaa acatcgttcc taactcaaca ggtgctgcta aagctatcgg tttagtaatt
    41521 cctgaattga acggtaaatt agacggagct gctcaacgtg ttcctgtacc aacaggttca
    41581 ttaacagaat tagtaacagt tcttgaaaaa gaagtaactg ttgacgaaat caatgcagca
    41641 atgaaagaag cttcaaacga atcttacgga tacaacacag acgaaatcgt ttcttcagat
    41701 atcgttggta tgacttacgg ttcattattt gatgctacac aaactaaagt aatgacagtt
    41761 ggcgacaaac aattagttaa aactgttgct tggtacgaca acgaaatgtc atacactgca
    41821 caattagtac gtactttaga atacttcgct aacttataag atagacaaac gtacaagtgt
    41881 aaattataga catgtacatt taaaagcggg gaagcaatcg cgcttcttcc gctttttttg
    41941 ataataagtt tattcaggag gaaataaaat ggctaagaaa acagtgaaag atatcgattt
    42001 aaaagacaaa aaagttcttg tccgtgttga cttcaacgtt cctttgaaag acggcgtgat
    42061 cactgacgac actcgtatca aagcagcatt gccaacaatc aactacgttc tagaacaagg
    42121 cggaaaagca atccttttct ctcaccttgg acgtgtaaaa acagaagaag ataaagaagg
    42181 aaaatcttta gctccagttg caaaacgtct aggcgaatta ttaggtaaag aagtaacatt
    42241 cgtacctgaa actcgcggag aacaattaga agaagcaatc cgcaacatga atgacggcga
    42301 cgttgtcgta ttcgaaaata cacgatttga agatattgac ggtaaaaaag aaagcaaaaa
    42361 tgatacagaa ctaggtaaat actgggcttc attaggcgat gtatttgtta acgatgcttt
    42421 cggtacagct caccgtgcac atgcttctaa cgtaggtatc gcttcaactg gtatcccaac
    42481 agttgctgga ttcttgatgg aaaaagaaat caagttcatc ggtgaagcag tagaagaacc
    42541 aaaacgacca atgattgcaa tccttggtgg cgcaaaagtt tctgataaaa tcggcgtaat
    42601 cgaaaacttg attcctaaag cagataaaat cttgatcggt ggcggaatga cttacacatt
    42661 ctacgaagca aaaggaatca agatcggtaa ctctctagta gaagctgata aagtggaact
    42721 agcaaaagaa ttaatcgaaa aagcaggcga caaacttgtc ttgccaatcg acaatgtctg
    42781 tgcaccagaa ttttcaaatg atgttgaaac acaagtaatc gaaggagaca ttcctgatgg
    42841 tttgatggct ttagacatcg gcccagcatc agtaaaatta tttgctgata cattacaagg
    42901 tgcaaaaaca gttgtttgga acggacctat gggtgtattt gaaatgagta actttgctaa
    42961 aggtacaatc ggtgtttgtg aagcaatcgc taacttagaa ggcgcaacaa caatcatcgg
    43021 tggcggagac tcagccgcag cagcagaaca attaggattt gctgacaaat tcacacacat
    43081 ctcaacaggt ggcggcgcaa gcttggaatt gcttgaaggt aaagaattac caggactagc
    43141 agcaatcaac gataaataaa cgaccaagtt tgctcaattc tgacgagtaa tcgatcaaaa
    43201 ggtgaaggga gttttcatga ctccctagcc atttttcaat aaattaagga gtgtgttcct
    43261 caatgcgtaa acctattatc gcaggtaact ggaaaatgaa caaaactgca tcagaagcaa
    43321 aagaatttgc tgaagcagta aaaaacaaaa tcccttcaaa tgacaaagtt gattcagtca
    43381 tcggatctcc tgcattattc ttacaagaat taatgacatc agctgaaggt acagaactaa
    43441 aaatctcagc acaaaactgc tactgggaaa actcaggtgc gttcactggt gaaacttcac
    43501 cagcagctct tgctgatcta ggtgttcatt atgtgatcat cggacattca gaacgtcgtg
    43561 aatatttcca tgaaacagac gaagacatca ataaaaaagc gaaagctatc tttgcaaatg
    43621 gtatgacacc aatcctatgc tgtggcgaat ctttagaaac ctatgaagca ggcaaaactg
    43681 ctgaatggat cgaaggacaa attacagctg gtttagctga tctttcagct gaacaagtgt
    43741 caaacatggt tattgcttat gaaccaatct gggcaatcgg tactggtaaa tcagctgacg
    43801 cacaaatcgc tgacgaaatt tgtggagttg tacgtcaaac agttgaaaaa ctttatggaa
    43861 aagaagtatc agaagcagtt cgtatccaat acggcggttc tgtaaaacca gaaaatattg
    43921 ctgaatacat ggcaaaagaa aatgttgacg gtgctttagt cggtggcgca agcttgcaag
    43981 cagattcatt cttgtcattg ttagacgctg taaaataatt gttgaaaatt tgcgaaaaat
    44041 gcaagaaatg tttgcactaa tgagcaaaat taactaaaat cgtatatgag ggaaatccct
    44101 taggaaaaaa caaactcaaa ggagagacaa aacatgtcaa ttattactga tgtttacgct
    44161 cgcgagatct tagactcacg tggtaaccca acaatcgaag tagaagtata cacagaaagc
    44221 ggagcttttg gccgcggaat ggttccttca ggtgcttcaa ctggtgaata cgaagcagtt
    44281 gaattacgtg acggagacaa agctcgttat ggcggtaaag gtgtaactaa agctgtcgac
    44341 aacgtaaaca acatcattgc tgaagcaatc attggctacg atgttcgtga ccaaatggct
    44401 atcgacaaag ctatgatcgc tttagacgga actcctaaca aaggtaaatt aggtgcgaac
    44461 gctattttag gtgtttctat cgctgttgca cgtgcagctg ctgactacct agaagtacct
    44521 ttataccact acctaggcgg attcaacaca aaagtattgc caacaccaat gatgaacatc
    44581 atcaacggcg gatcacatgc tgataactca atcgacttcc aagaattcat gatcatgcct
    44641 gttggcgctc caacattcaa agaagctcta cgtatgggtg ctgaagtatt ccacgcatta
    44701 gcttcaatct tgaaagctcg cggattagca acttctgttg gtgacgaagg tggatttgct
    44761 ccaaaccttg gttcaaacga agaaggtttt gaagtaatca tcgaagcaat cgaaaaagct
    44821 ggctatgtac ctggtaaaga cgttgttctt gctatggatg ctgcttcttc agaattctac
    44881 gacaaagaaa aaggtgttta cgtactagct gattcaggcg aaggcgaaaa aacaactgac
    44941 gaaatgatca aattctacga agaattagtt tctaaatatc caatcatctc aatcgaagac
    45001 ggattagacg aaaacgactg ggacggattc aagaaattaa ctgacgtatt aggcgacaaa
    45061 gttcaattag ttggtgacga cttgttcgta acaaacactc aaaaattatc tgaaggtatc
    45121 gaaaaaggaa ttgctaactc aatccttatc aaagtgaacc aaatcggtac attaacagaa
    45181 actttcgaag ctatcgaaat ggctaaagaa gctggctaca cagcagttgt atctcaccgt
    45241 tctggtgaaa cagaagattc aacaatctct gatatcgctg ttgcaacaaa cgctggccaa
    45301 atcaaaactg gttctctatc acgtactgac cgtattgcta aatacaacca attactacgt
    45361 atcgaagacc aacttggtga agttgcagaa tacaaaggtt tgaaatcttt ctacaactta
    45421 aaaaacaaat aattcttttc taatgaagat acatgatcga ctattgaaag atagtcgagt
    45481 atgatcactt agttaagtaa tcgaactctc tacttgctga aatatcagca ggtagagagt
    45541 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
    45601 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
    45661 gtagaatagg aatctatgag caagtcctct cgtataaata aaataggagg gaagtagcag
    45721 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
    45781 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
    45841 gaagaaaaaa tcgcgatttt acaagagatc atccgaatta aatcagtgaa tggaaacgaa
    45901 ggtgaagtag cagcctattt gaataagctt ttggcaaagc atgacataac aggagagatt
    45961 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
    46021 aaagtgttag gcctatctgg gcatatggat gtcgtggcgg ctggtgatga atcgtcttgg
    46081 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
    46141 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
    46201 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
    46261 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
    46321 gagccaacta attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
    46381 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
    46441 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
    46501 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
    46561 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
    46621 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
    46681 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
    46741 cctttgattc attgcattca gcaacaattc agccagccat tacctttagt tggagcggca
    46801 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
    46861 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
    46921 ttggatatga ttgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
    46981 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
    47041 gcctattttt ctttttgaat atatcaagcg ttgtggcaac atagtgatga ctaatgaaac
    47101 aacgacgata gacaacaaac gatcaaggta gtctgtacct gcctgtacca agatcacact
    47161 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
    47221 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
    47281 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
    47341 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
    47401 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
    47461 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
    47521 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
    47581 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
    47641 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
    47701 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
    47761 aacgatcaac caatgtttgt ccacgactta ttccttgagt ttccacagca gtgaagcttt
    47821 tgaaaccttc acagatggcc ccctcgataa aataagcaac agctaacgga tcattgatga
    47881 cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga gtgatcgctt
    47941 ttagatactc tccctcttca ggattcattt gacagcagta ttcgaggata gtcggtgtca
    48001 agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg agattctcga
    48061 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggcgaacagt
    48121 taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt ttcccaatat
    48181 ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca atgactgatg
    48241 tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga ggttctgcct
    48301 gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca tgagtgtctt
    48361 gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca ggaatgtcta
    48421 aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct gcagggacat
    48481 tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca gctaatgata
    48541 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc attatttcca
    48601 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
    48661 acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc gaatgtaaat
    48721 gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa tgaaaaagaa
    48781 atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg gataatagtt
    48841 agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt tcattcttat
    48901 tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa agaaattagt
    48961 aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc gtttgatgga
    49021 ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag agtgtgaaag
    49081 cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga aactagctgg
    49141 tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac atgattaccg
    49201 aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
    49261 cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca tcggaatggg
    49321 atggctagat cggctagatt taattgcctg gatccaactc atcttagatg aacagccgaa
    49381 tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga tggcaagtgg
    49441 tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg tttctgttta
    49501 tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa tggtcatgag
    49561 atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag catcagctac
    49621 gagacagctt ggcagcaacc atctgccttt attggtcatt catggtgaaa gagatcattt
    49681 tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata aagcactgct
    49741 gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa cctattggac
    49801 agtgattttt tcatttattg aacaaagaat aacgatgaat taattttctc ttcttttttg
    49861 taagtaattc gaggataaat aattgtttat agatcaggag actgctaaaa taaggtagga
    49921 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa gaaaaaaatc
    49981 gtcttgcttt attcaatgat gaacaacaag agatcggaga aatgacatgg tcagatgcgg
    50041 gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt ggtcaaaagt
    50101 tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg aaaaagatca
    50161 ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat caagacgtct
    50221 tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta ttccatttat
    50281 atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc ttttgggaag
    50341 gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
    50401 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
    50461 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
    50521 caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca tatttatctt
    50581 cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt gcaaagagaa
    50641 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgagacgag
    50701 tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt ttgctgcttc
    50761 acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg gaaaaagcag
    50821 attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca tatgatattt
    50881 tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat tttttacagt
    50941 ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc tttgctgtac
    51001 gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct ttgtctcctg
    51061 taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg aagacagcag
    51121 gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta gctgaacaac
    51181 aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca gatatccaac
    51241 agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat ggcgtatttg
    51301 aaactgcacg caagttgagc cgtcagcgag tgacacttcg atggtatcct gaaagtggtc
    51361 atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg gaatttttag
    51421 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag agaaaattct
    51481 ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg ctgaaggttt
    51541 agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
    51601 agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa aaccaatctt
    51661 gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga ttgatcccga
    51721 agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg gagacaccgt
    51781 agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag aggggaaagt
    51841 agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg cttattcaga
    51901 agaagaaata caagaaacgg atctttacgg cgttgttatc ccgaaagata gaaagctcaa
    51961 ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa gtattgtgct
    52021 gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag ggataatcaa
    52081 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg tcgtggctaa
    52141 tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag ttccagatca
    52201 aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca ttgttacgat
    52261 tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat tagataacgg
    52321 taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac aaggcagtca
    52381 attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc gagtgattcc
    52441 gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg ttccaaggct
    52501 gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc atgatatttt
    52561 cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
    52621 agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt tccaagaaat
    52681 gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga tttcttttga
    52741 ggatcgcgaa gcaaaaatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
    52801 aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca atgaaacggt
    52861 agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg aacagccaaa
    52921 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
    52981 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
    53041 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
    53101 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
    53161 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
    53221 aagtgaaagc aatcaaagtt cgtgggctac ggaacttcct gaaattgcag atcatagttc
    53281 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
    53341 attcatgatg gacaaagtgg gggaagaatt cgatgggatc atcagctctg ttgtgaaatt
    53401 cggcctgttt atcgaactgc cgaatacagt agagggattg atccatatca atgaattgaa
    53461 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
    53521 gacttttaaa atcggacaga aagtgcgagt aaaagtagtc aaagctgatc cagatgaacg
    53581 agcagtagat tttgaacttg tttccgcaga agaggttaca gcgttggaac ggccaaaaac
    53641 acgaaatgga aaatcaggaa aacgcgaagg aaaacaagca ggaaaacgcc cagaaagaaa
    53701 tggcaagaag aacaaacgat atagcgatag taagcaaacg ttcggtaaga aaaaagggaa
    53761 gaagccattt tacaaaggtg ttaaaaagaa gaaaaagaaa aaataaaatg agttggagga
    53821 aacagctatg ccaaaaggcg agggaaaatt aattgcacaa aacaagaaag ctcgccatga
    53881 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaaccg agatcaagtc
    53941 gatacgaaac agccggatca atctaaaaga tggatttatt cgcgtccgca acggagaagc
    54001 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
    54061 gttgcgcacg agaaagttat tattacacaa aaaacaaatc atccggcttg aaaatgaatt
    54121 gaaaaatact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
    54181 ggtattgatt ggtctggcga aagggaaaaa atcttatgat aaacgggaag atttgaaacg
    54241 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
    54301 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
    54361 atctgataaa acatgattat cataggtttt ttcttagatc aggagcaccg tgtataaaga
    54421 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
    54481 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
    54541 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
    54601 aacgctcgct aacgttccac attggaccaa tttggtttga tggtacagtt tgtataatgg
    54661 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
    54721 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
    54781 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
    54841 ttttatttgt ttttgttgca aacaacatcg gtttagtaac aaaaatcgtt ttacctaacg
    54901 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
    54961 taatgatctt acttactcac ttatttgggg tgaaaaagtt aggcttcaaa ggatatttcg
    55021 taaattcgtt cttgaaacca tatagtttta tgtttccgat gaaactgatt gaagaattta
    55081 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
    55141 taaccttgat tgcaaaaatg gtaataagct taggctggtt ctcattgccg ttagcaattc
    55201 cattagagat ggtctggatc gcattctcct tgtttatcgg aagcatccaa gcatttgtct
    55261 tcgttacttt atctatggtt tatatgagcc ataagattga agtggaagaa taatattcgt
    55321 tttaaaaaca aactactatt tatctaattt aggaggaaaa caattatgaa ttatatcgca
    55381 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
    55441 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
    55501 atgtttatcg gggtagctct agtcgaagct gttcctatct taggggtagt tgtcgccttg
    55561 attctagttt ttggtgttta attgaatcag tattaaggca gaaacaattt ggtttgttac
    55621 tgctttttta catgaaaact agaagtactt caaagaaagg aaggctcgcc tatgctgaat
    55681 caattggcaa ttgcagaagt tggcaatgcg atgttaggaa atgtcatcgt tgtcagcggc
    55741 tcatttttga tactactggc tctcttgaag cattttgctt ggggaccaat cagcgatatt
    55801 ttgaaaaaac gtgaagacaa gatcgccaat gatttggatt ctgcagaaca atctcgcatc
    55861 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    55921 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    55981 gatgctcaag aagaagcagc tcgtctaaaa agtaaagccc aagctgatat cactgtagaa
    56041 cgtgattcag cgctgaactc tgtaaaagac gatgttgcag aactatctct tcaaatcgcg
    56101 gaaaaaatct tgaacaaaga attatctcca gaaatgcacg aatcattaat caatcaatat
    56161 atcgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    56221 gaaagcttta tttgaactgg cgatcgattc aaataaagca gaagaaattt atcaagatct
    56281 cgtaagctta cgtcaaatct atgaacaagt acctggatta ggtaatatgt taagtgatgt
    56341 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgttagtg gctatgatgg
    56401 aatcgttaaa aactttttag aagttgttta tgcgtataac cgaatggatg atcttttgtt
    56461 catgatcgaa gaatatgaac gtcgatatga tgaacatcaa ggtttgctgt tgggaagtgt
    56521 cacaacagca gttccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    56581 aacaatgaac tatcaatcag tcgaattaaa acaaatcgtt gattcttcta ttattggtgg
    56641 cgcgattgtt gaagctaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    56701 tatgcgtaat caattaaata gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    56761 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    56821 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    56881 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    56941 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    57001 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgattg
    57061 gacgggtagt taatccgcta ggtcagccaa ttgacggact aggtgaaatc gttactgaca
    57121 aagctcgtcc agtagaagca atggcaccag gcgttatgca acgtaaatct gttaacgaac
    57181 caatgcaaac aggtctaaaa gcaatcgacg ctcttgtacc aatcggacgc ggccaacgtg
    57241 aattagttat cggtgaccgt aaaacaggga aaacttccat tgcgatcgat acgatcatca
    57301 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    57361 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    57421 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    57481 tgggtgaaga gttcatgtac aacgggaaac atgtcttgat catttttgat gatctttcaa
    57541 aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca ggtcgtgaag
    57601 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    57661 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    57721 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    57781 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    57841 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    57901 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    57961 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    58021 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    58081 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    58141 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    58201 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    58261 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    58321 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    58381 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    58441 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    58501 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgttgctc
    58561 agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg tttagtcggt
    58621 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    58681 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    58741 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    58801 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    58861 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    58921 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    58981 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    59041 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    59101 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa ccgtgcaaga
    59161 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    59221 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    59281 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    59341 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    59401 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    59461 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    59521 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    59581 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    59641 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    59701 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    59761 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    59821 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    59881 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    59941 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    60001 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    60061 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    60121 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    60181 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    60241 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    60301 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    60361 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    60421 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    60481 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    60541 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    60601 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    60661 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    60721 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    60781 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    60841 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    60901 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    60961 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    61021 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    61081 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    61141 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    61201 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg gaggttgtga
    61261 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    61321 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    61381 ctgcgacaag ac
//
LOCUS       DACEMS010000004        55486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEMS010000004 DACEMS010000000
VERSION     DACEMS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.004"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            117..971
                     /locus_tag="IW735_000203"
     CDS             117..971
                     /locus_tag="IW735_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1043234.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            972..2309
                     /locus_tag="IW735_000204"
     CDS             972..2309
                     /locus_tag="IW735_000204"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1043235.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2314..2538
                     /locus_tag="IW735_000205"
     CDS             2314..2538
                     /locus_tag="IW735_000205"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1043236.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2540..3430
                     /locus_tag="IW735_000206"
     CDS             2540..3430
                     /locus_tag="IW735_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1043237.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3439..4257
                     /locus_tag="IW735_000207"
     CDS             3439..4257
                     /locus_tag="IW735_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1043238.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4364..4813
                     /locus_tag="IW735_000208"
     CDS             4364..4813
                     /locus_tag="IW735_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1043239.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4831..6513
                     /gene="recN"
                     /locus_tag="IW735_000209"
     CDS             4831..6513
                     /gene="recN"
                     /locus_tag="IW735_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1043240.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6568..6684)
                     /locus_tag="IW735_000210"
     CDS             complement(6568..6684)
                     /locus_tag="IW735_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1043241.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6757..7713)
                     /locus_tag="IW735_000211"
     CDS             complement(6757..7713)
                     /locus_tag="IW735_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1043242.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7884..8252
                     /locus_tag="IW735_000212"
     CDS             7884..8252
                     /locus_tag="IW735_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1043243.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8422..8853
                     /gene="mraZ"
                     /locus_tag="IW735_000213"
     CDS             8422..8853
                     /gene="mraZ"
                     /locus_tag="IW735_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1043244.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8874..9833
                     /gene="rsmH"
                     /locus_tag="IW735_000214"
     CDS             8874..9833
                     /gene="rsmH"
                     /locus_tag="IW735_000214"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1043245.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9838..10239
                     /gene="ftsL"
                     /locus_tag="IW735_000215"
     CDS             9838..10239
                     /gene="ftsL"
                     /locus_tag="IW735_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1043246.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10240..12432
                     /locus_tag="IW735_000216"
     CDS             10240..12432
                     /locus_tag="IW735_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1043247.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12458..13420
                     /locus_tag="IW735_000217"
     CDS             12458..13420
                     /locus_tag="IW735_000217"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1043248.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13434..14795
                     /locus_tag="IW735_000218"
     CDS             13434..14795
                     /locus_tag="IW735_000218"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1043249.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14821..15909
                     /gene="murG"
                     /locus_tag="IW735_000219"
     CDS             14821..15909
                     /gene="murG"
                     /locus_tag="IW735_000219"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1043250.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15924..17144
                     /locus_tag="IW735_000220"
     CDS             15924..17144
                     /locus_tag="IW735_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1043251.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17300..18625
                     /gene="ftsA"
                     /locus_tag="IW735_000221"
     CDS             17300..18625
                     /gene="ftsA"
                     /locus_tag="IW735_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1043252.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW735_000222"
     CDS             18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW735_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1043253.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19906..20583
                     /locus_tag="IW735_000223"
     CDS             19906..20583
                     /locus_tag="IW735_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1043254.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20597..21196
                     /locus_tag="IW735_000224"
     CDS             20597..21196
                     /locus_tag="IW735_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1043255.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21211..21498
                     /locus_tag="IW735_000225"
     CDS             21211..21498
                     /locus_tag="IW735_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1043256.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21547..22329
                     /locus_tag="IW735_000226"
     CDS             21547..22329
                     /locus_tag="IW735_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1043257.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22350..23057
                     /locus_tag="IW735_000227"
     CDS             22350..23057
                     /locus_tag="IW735_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1043258.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23319..26117
                     /gene="ileS"
                     /locus_tag="IW735_000228"
     CDS             23319..26117
                     /gene="ileS"
                     /locus_tag="IW735_000228"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1043259.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26166..27692)
                     /locus_tag="IW735_000229"
     CDS             complement(26166..27692)
                     /locus_tag="IW735_000229"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1043260.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27707..28354)
                     /locus_tag="IW735_000230"
     CDS             complement(27707..28354)
                     /locus_tag="IW735_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1043261.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28538..28867)
                     /locus_tag="IW735_000231"
     CDS             complement(28538..28867)
                     /locus_tag="IW735_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1043262.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29044..29772
                     /gene="sfsA"
                     /locus_tag="IW735_000232"
     CDS             29044..29772
                     /gene="sfsA"
                     /locus_tag="IW735_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1043263.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29788..30801
                     /locus_tag="IW735_000233"
     CDS             29788..30801
                     /locus_tag="IW735_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1043264.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30801..32078
                     /locus_tag="IW735_000234"
     CDS             30801..32078
                     /locus_tag="IW735_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1043265.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32141..34843
                     /locus_tag="IW735_000235"
     CDS             32141..34843
                     /locus_tag="IW735_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1043266.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34995..35312
                     /locus_tag="IW735_000236"
     CDS             34995..35312
                     /locus_tag="IW735_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1043267.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35342..35662
                     /locus_tag="IW735_000237"
     CDS             35342..35662
                     /locus_tag="IW735_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1043268.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35748..37208
                     /locus_tag="IW735_000238"
     CDS             35748..37208
                     /locus_tag="IW735_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1043269.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37276..37497
                     /locus_tag="IW735_000239"
     CDS             37276..37497
                     /locus_tag="IW735_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043270.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37528..37710
                     /locus_tag="IW735_000240"
     CDS             37528..37710
                     /locus_tag="IW735_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1043271.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37710..38123
                     /locus_tag="IW735_000241"
     CDS             37710..38123
                     /locus_tag="IW735_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1043272.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38246..39427
                     /locus_tag="IW735_000242"
     CDS             38246..39427
                     /locus_tag="IW735_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1043273.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            39816..39889
                     /locus_tag="IW735_000243"
     tRNA            39816..39889
                     /locus_tag="IW735_000243"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39850..39852,aa:Arg,seq:cct)
     gene            complement(39926..40126)
                     /locus_tag="IW735_000244"
     CDS             complement(39926..40126)
                     /locus_tag="IW735_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1043274.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(40431..41663)
                     /locus_tag="IW735_000245"
     CDS             complement(40431..41663)
                     /locus_tag="IW735_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1043275.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            41920..42489
                     /locus_tag="IW735_000246"
     CDS             41920..42489
                     /locus_tag="IW735_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1043276.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(42667..43107)
                     /locus_tag="IW735_000247"
     CDS             complement(42667..43107)
                     /locus_tag="IW735_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1043277.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            43265..44029
                     /gene="map"
                     /locus_tag="IW735_000248"
     CDS             43265..44029
                     /gene="map"
                     /locus_tag="IW735_000248"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1043278.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            44061..44984
                     /locus_tag="IW735_000249"
     CDS             44061..44984
                     /locus_tag="IW735_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1043279.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            45060..46199
                     /locus_tag="IW735_000250"
     CDS             45060..46199
                     /locus_tag="IW735_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1043280.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            46192..46992
                     /locus_tag="IW735_000251"
     CDS             46192..46992
                     /locus_tag="IW735_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1043281.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            46992..47819
                     /locus_tag="IW735_000252"
     CDS             46992..47819
                     /locus_tag="IW735_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1043282.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            47797..48531
                     /locus_tag="IW735_000253"
     CDS             47797..48531
                     /locus_tag="IW735_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1043283.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            48631..49497
                     /gene="rfbA"
                     /locus_tag="IW735_000254"
     CDS             48631..49497
                     /gene="rfbA"
                     /locus_tag="IW735_000254"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1043284.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYGNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            49511..50083
                     /gene="rfbC"
                     /locus_tag="IW735_000255"
     CDS             49511..50083
                     /gene="rfbC"
                     /locus_tag="IW735_000255"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1043285.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            50105..51133
                     /gene="rfbB"
                     /locus_tag="IW735_000256"
     CDS             50105..51133
                     /gene="rfbB"
                     /locus_tag="IW735_000256"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1043286.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            51231..52082
                     /gene="rfbD"
                     /locus_tag="IW735_000257"
     CDS             51231..52082
                     /gene="rfbD"
                     /locus_tag="IW735_000257"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1043287.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            52116..54149
                     /locus_tag="IW735_000258"
     CDS             52116..54149
                     /locus_tag="IW735_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043288.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            54193..55473
                     /locus_tag="IW735_000259"
     CDS             54193..55473
                     /locus_tag="IW735_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1043289.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT        18108 a         9494 c        11819 g        16065 t
ORIGIN      
        1 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
       61 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
      121 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
      181 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
      241 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
      301 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
      361 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
      421 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
      481 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
      541 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
      601 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
      661 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
      721 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
      781 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
      841 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
      901 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
      961 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
     1021 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
     1081 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
     1141 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
     1201 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
     1261 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
     1321 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
     1381 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
     1441 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
     1501 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
     1561 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
     1621 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
     1681 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
     1741 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
     1801 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
     1861 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
     1921 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
     1981 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
     2041 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
     2101 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
     2161 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
     2221 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
     2281 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
     2341 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
     2401 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
     2461 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
     2521 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
     2581 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
     2641 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
     2701 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
     2761 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
     2821 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
     2881 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
     2941 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
     3001 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
     3061 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
     3121 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
     3181 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
     3241 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
     3301 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
     3361 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
     3421 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
     3481 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
     3541 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
     3601 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
     3661 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
     3721 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
     3781 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
     3841 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
     3901 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
     3961 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
     4021 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
     4081 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
     4141 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
     4201 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
     4261 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
     4321 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
     4381 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
     4441 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
     4501 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
     4561 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
     4621 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
     4681 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
     4741 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
     4801 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
     4861 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
     4921 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
     4981 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
     5041 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
     5101 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
     5161 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
     5221 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
     5281 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
     5341 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
     5401 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
     5461 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
     5521 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
     5581 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
     5641 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
     5701 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
     5761 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
     5821 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
     5881 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
     5941 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
     6001 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
     6061 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
     6121 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
     6181 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
     6241 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
     6301 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
     6361 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
     6421 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
     6481 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
     6541 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
     6601 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
     6661 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
     6721 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
     6781 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
     6841 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
     6901 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
     6961 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
     7021 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
     7081 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
     7141 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
     7201 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
     7261 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
     7321 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
     7381 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
     7441 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
     7501 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
     7561 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
     7621 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
     7681 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
     7741 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
     7801 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
     7861 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
     7921 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
     7981 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
     8041 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
     8101 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
     8161 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
     8221 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
     8281 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
     8341 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
     8401 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
     8461 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
     8521 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
     8581 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
     8641 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
     8701 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
     8761 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
     8821 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
     8881 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
     8941 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
     9001 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
     9061 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
     9121 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
     9181 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
     9241 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
     9301 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
     9361 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
     9421 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
     9481 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
     9541 ccgaagaggc ctctttagaa caggcaatcg ggttattgaa agtcaatgga agaatcagcg
     9601 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
     9661 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
     9721 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
     9781 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
     9841 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
     9901 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
     9961 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    10021 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    10081 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    10141 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    10201 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    10261 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    10321 attttatttg ctacgagtat tggattattc tttttatttg ccgtccgttt ctcgtatatc
    10381 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    10441 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    10501 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    10561 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    10621 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    10681 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    10741 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    10801 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    10861 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    10921 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    10981 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    11041 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    11101 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    11161 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    11221 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    11281 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    11341 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    11401 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    11461 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    11521 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    11581 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    11641 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    11701 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    11761 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    11821 gtacctggat acaatgtatc ggcaaaaacc ggaacagccc aaatcgcttc tgatacaggg
    11881 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    11941 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    12001 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    12061 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    12121 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    12181 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    12241 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    12301 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    12361 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    12421 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    12481 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    12541 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    12601 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    12661 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    12721 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    12781 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    12841 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    12901 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    12961 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    13021 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    13081 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    13141 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    13201 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    13261 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    13321 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    13381 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    13441 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    13501 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    13561 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    13621 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    13681 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    13741 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    13801 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    13861 cacgcttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    13921 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    13981 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    14041 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    14101 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    14161 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    14221 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    14281 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    14341 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    14401 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    14461 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    14521 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    14581 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    14641 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    14701 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    14761 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    14821 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    14881 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    14941 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    15001 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    15061 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    15121 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    15181 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    15241 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    15301 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    15361 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    15421 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    15481 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    15541 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    15601 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    15661 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    15721 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    15781 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    15841 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    15901 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    15961 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    16021 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    16081 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    16141 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    16201 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    16261 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    16321 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    16381 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    16441 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    16501 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    16561 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    16621 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    16681 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    16741 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    16801 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    16861 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    16921 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    16981 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    17041 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    17101 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    17161 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    17221 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    17281 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    17341 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    17401 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    17461 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    17521 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    17581 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    17641 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    17701 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    17761 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    17821 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    17881 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    17941 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    18001 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    18061 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    18121 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    18181 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    18241 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    18301 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    18361 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    18421 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    18481 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    18541 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    18601 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    18661 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    18721 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    18781 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    18841 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    18901 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    18961 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    19021 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    19081 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    19141 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    19201 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    19261 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    19321 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    19381 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    19441 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    19501 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    19561 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    19621 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    19681 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    19741 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    19801 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    19861 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    19921 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    19981 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    20041 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    20101 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    20161 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    20221 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    20281 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    20341 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    20401 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    20461 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    20521 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    20581 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    20641 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    20701 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    20761 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    20821 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    20881 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    20941 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    21001 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    21061 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    21121 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    21181 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    21241 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    21301 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    21361 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    21421 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    21481 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    21541 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    21601 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    21661 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    21721 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    21781 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    21841 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    21901 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    21961 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    22021 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    22081 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    22141 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    22201 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    22261 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    22321 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    22381 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    22441 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    22501 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    22561 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    22621 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    22681 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    22741 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    22801 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    22861 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    22921 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    22981 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    23041 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    23101 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    23161 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    23221 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    23281 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    23341 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    23401 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    23461 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    23521 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    23581 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    23641 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    23701 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    23761 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    23821 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    23881 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    23941 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    24001 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    24061 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    24121 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    24181 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    24241 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    24301 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    24361 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    24421 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    24481 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    24541 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    24601 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    24661 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    24721 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    24781 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    24841 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    24901 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    24961 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    25021 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    25081 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    25141 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    25201 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    25261 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    25321 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    25381 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    25441 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    25501 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    25561 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    25621 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    25681 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    25741 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    25801 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    25861 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    25921 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    25981 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    26041 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    26101 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    26161 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    26221 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    26281 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    26341 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    26401 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    26461 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    26521 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    26581 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    26641 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    26701 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    26761 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    26821 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    26881 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    26941 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    27001 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    27061 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    27121 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    27181 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    27241 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    27301 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    27361 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    27421 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    27481 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    27541 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    27601 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    27661 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    27721 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    27781 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    27841 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    27901 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    27961 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    28021 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    28081 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    28141 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    28201 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    28261 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    28321 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    28381 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    28441 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    28501 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    28561 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    28621 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    28681 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    28741 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    28801 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    28861 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    28921 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    28981 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    29041 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    29101 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    29161 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    29221 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    29281 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    29341 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    29401 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    29461 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    29521 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    29581 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    29641 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    29701 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    29761 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    29821 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    29881 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    29941 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    30001 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    30061 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    30121 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    30181 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    30241 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    30301 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    30361 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    30421 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    30481 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    30541 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    30601 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    30661 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    30721 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    30781 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    30841 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    30901 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    30961 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    31021 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    31081 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    31141 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    31201 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    31261 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    31321 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    31381 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    31441 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    31501 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    31561 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    31621 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    31681 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    31741 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    31801 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    31861 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    31921 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    31981 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    32041 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    32101 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    32161 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    32221 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    32281 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    32341 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    32401 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    32461 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    32521 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    32581 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    32641 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    32701 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    32761 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    32821 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    32881 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    32941 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    33001 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    33061 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    33121 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    33181 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    33241 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    33301 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    33361 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    33421 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    33481 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    33541 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    33601 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    33661 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    33721 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    33781 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    33841 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    33901 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    33961 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    34021 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    34081 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    34141 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    34201 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    34261 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    34321 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    34381 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    34441 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    34501 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    34561 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    34621 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    34681 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    34741 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    34801 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    34861 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    34921 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    34981 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    35041 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    35101 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    35161 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    35221 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    35281 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    35341 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    35401 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    35461 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    35521 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    35581 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    35641 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggattttt tattttgtta
    35701 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    35761 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    35821 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    35881 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    35941 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    36001 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    36061 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    36121 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    36181 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    36241 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    36301 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    36361 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    36421 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    36481 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    36541 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    36601 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    36661 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    36721 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    36781 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    36841 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    36901 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    36961 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    37021 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    37081 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    37141 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    37201 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    37261 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    37321 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    37381 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    37441 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    37501 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    37561 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    37621 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    37681 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    37741 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    37801 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    37861 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    37921 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    37981 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    38041 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    38101 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    38161 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    38221 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    38281 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    38341 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    38401 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    38461 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    38521 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    38581 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    38641 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    38701 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    38761 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    38821 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    38881 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    38941 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    39001 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    39061 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    39121 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    39181 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    39241 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    39301 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    39361 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    39421 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    39481 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    39541 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    39601 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    39661 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    39721 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    39781 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    39841 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt acaaataacc
    39901 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
    39961 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
    40021 atgcatgcgt atgtcttagt ccctcaataa acagtcgaag agaaaatagg caaaaattga
    40081 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caacagattc
    40141 gaattaaacg atttcgattg gcttatacca gtcctatccg acgaaagata tatcgttctt
    40201 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
    40261 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
    40321 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
    40381 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
    40441 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
    40501 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
    40561 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
    40621 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
    40681 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
    40741 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aatgactttt
    40801 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
    40861 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
    40921 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
    40981 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg cagggcagaa
    41041 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
    41101 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
    41161 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
    41221 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
    41281 gctttgccag ccgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
    41341 tcgggatcgg cagaaacaac actgatcctg cttgctcctt tttcaagcca ttcatccgct
    41401 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
    41461 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
    41521 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
    41581 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
    41641 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
    41701 ttagcatgat accatttaat tatgattcaa acaatgaggc ctttaaataa aaaaaagagg
    41761 ttattttttt aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa
    41821 gaaaatcaaa aactaatttt gcatatcaaa aaaaacatgt tacaatgaaa ttaatcaatg
    41881 gtggaagcgt tataaaaaaa ttaaggagat taaaaaaata tggcaagaaa aaagacgatt
    41941 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
    42001 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
    42061 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
    42121 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
    42181 atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt
    42241 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
    42301 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
    42361 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
    42421 attgaaaaga tgatacaaga cagtatagat gctatcttga aattcaaaga accaatagaa
    42481 atcgattgaa tatactcaaa agttccacaa atcagtagat aacaagagat gtgtcaaaat
    42541 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
    42601 gaatgcaatg aataaaagag ccgtacagat tgaataaatc tggatggctc ttttattgca
    42661 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
    42721 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
    42781 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
    42841 tccgcagaca ccgtaaattt ttcccgatag atccaattct tgcaagtctt cataaaagtc
    42901 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
    42961 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
    43021 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
    43081 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
    43141 tatgaattat accaaaacat cattgaaaag aaaataaaac ctagagacac ctggggaatg
    43201 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
    43261 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
    43321 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
    43381 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
    43441 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
    43501 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
    43561 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
    43621 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
    43681 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
    43741 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
    43801 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
    43861 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
    43921 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
    43981 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
    44041 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
    44101 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
    44161 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
    44221 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
    44281 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
    44341 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
    44401 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
    44461 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
    44521 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
    44581 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
    44641 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
    44701 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
    44761 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
    44821 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
    44881 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
    44941 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
    45001 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
    45061 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
    45121 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
    45181 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
    45241 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
    45301 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
    45361 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
    45421 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
    45481 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
    45541 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
    45601 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
    45661 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
    45721 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
    45781 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
    45841 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
    45901 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
    45961 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
    46021 cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
    46081 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
    46141 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
    46201 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
    46261 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
    46321 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
    46381 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
    46441 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
    46501 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
    46561 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
    46621 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
    46681 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
    46741 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
    46801 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
    46861 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
    46921 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
    46981 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
    47041 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
    47101 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
    47161 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
    47221 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
    47281 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
    47341 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
    47401 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
    47461 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
    47521 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
    47581 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
    47641 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
    47701 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
    47761 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
    47821 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
    47881 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
    47941 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
    48001 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
    48061 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
    48121 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
    48181 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
    48241 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
    48301 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
    48361 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
    48421 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
    48481 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
    48541 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
    48601 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
    48661 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
    48721 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
    48781 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
    48841 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
    48901 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
    48961 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
    49021 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
    49081 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
    49141 actggccttt atttttatgg caatgaggta atttctatcg ctaaaggtat caaaccttca
    49201 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
    49261 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
    49321 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
    49381 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
    49441 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
    49501 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
    49561 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
    49621 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
    49681 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
    49741 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
    49801 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
    49861 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
    49921 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
    49981 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
    50041 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
    50101 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
    50161 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
    50221 aggaaacaaa gagaacttgg ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga
    50281 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
    50341 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
    50401 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
    50461 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
    50521 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
    50581 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
    50641 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
    50701 tcctcgccaa gtaacgaata tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
    50761 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
    50821 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
    50881 cgtgatggaa ttgatcttag aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa
    50941 cgaccgtgca ggacacgact tgcgttatgc aatcgattcg acaaaattac gggaagagtt
    51001 aggatggaag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
    51061 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
    51121 aaatggacaa taatacagag aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag
    51181 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataatcat atgtttttat
    51241 taactggagg aaatgggcaa ctaggtacag aattgcgcca tttattagat gaaaaaaatc
    51301 tgcaatatgt ttcaacagat gcaaaagact tggatatcac agatgccgag aaaacaatgg
    51361 cttacatcac agaattaaaa cctgatgtta ttttccattg tgcagcttac acagcagtag
    51421 ataaagcaga agaagaagca aaagaattag atgagaagat caatgttgat ggtacaagaa
    51481 atgtagcact tgcagcaaaa gctgtgaatg ctcaattcgt ctatatcagt acagactatg
    51541 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
    51601 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
    51661 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
    51721 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
    51781 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
    51841 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa
    51901 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
    51961 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagagtta ggatttgtca
    52021 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
    52081 aatttccttt tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac
    52141 gacgaaaaga aaagccaaaa gcgaaccagg cagattcgaa tttggtgctt gacctgtttc
    52201 taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa
    52261 acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa
    52321 ctgatgtgat ttttggacag aatcgggagg tgcggtcaga cgaatggctg gtgcagacac
    52381 ctttttacct ctcacaagct gaagaggact ttccgttggt gaacgaggat tattcattaa
    52441 acggacaaaa catgatcatt gcgtataatt ctcctgcaaa ggatattacg gttatcggaa
    52501 aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg
    52561 cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga
    52621 gaaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt
    52681 ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg atgactgctt
    52741 tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg
    52801 tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg
    52861 cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat
    52921 gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg
    52981 ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag
    53041 gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa
    53101 attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct
    53161 atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata
    53221 gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct atcttttgga
    53281 ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat
    53341 taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt
    53401 atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg
    53461 catggtctta tttctatgcg attacgcatt cgaaaataga taattatttt tcggattttg
    53521 aaaccatcgc agttgctgtg ttgggaacag tgattttagc atttgcatta tttggctgga
    53581 gacgcttgtt ttacctcgct tttgccgcct tagttatcgt atctggcttt ttcgtcaatc
    53641 cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa
    53701 tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc
    53761 caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga
    53821 aggaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag
    53881 cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca
    53941 cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat
    54001 taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag
    54061 acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa
    54121 ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta
    54181 gtagaaagga gagtggagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt
    54241 gtcggtttct tgcttttgtt gctgacgcta gtcttttttg tttggacagc agggttaggt
    54301 tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct
    54361 attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt
    54421 gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt
    54481 ggagatttac tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg
    54541 acaaaatggg atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta
    54601 tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat
    54661 tcgctttctg atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct
    54721 ttattgatca gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca
    54781 gtagcagttg ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc
    54841 gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt
    54901 gcgccattta ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg
    54961 gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat
    55021 cagacgctag gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccaa
    55081 cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga
    55141 ttgataggat ttgggattgc tactttgctg cttatccttt ttattggtcg cttaacgttg
    55201 aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg
    55261 cagtcattta tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg
    55321 ttggctatgg ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg
    55381 gaagaggcat atgacgatat gacttatgat gatacatctt atggggaaca accagatagc
    55441 gctcattctt ccaggatcca acgctatcat tgataggcac aaaata
//
LOCUS       DACEMS010000005        55278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEMS010000005 DACEMS010000000
VERSION     DACEMS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.005"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            410..1540
                     /locus_tag="IW735_000260"
     CDS             410..1540
                     /locus_tag="IW735_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1043290.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1632..2387
                     /locus_tag="IW735_000261"
     CDS             1632..2387
                     /locus_tag="IW735_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1043291.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2445..4484
                     /locus_tag="IW735_000262"
     CDS             2445..4484
                     /locus_tag="IW735_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1043292.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4593..5744)
                     /locus_tag="IW735_000263"
     CDS             complement(4593..5744)
                     /locus_tag="IW735_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1043293.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5898..6428)
                     /locus_tag="IW735_000264"
     CDS             complement(5898..6428)
                     /locus_tag="IW735_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043294.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6527..6877)
                     /locus_tag="IW735_000265"
     CDS             complement(6527..6877)
                     /locus_tag="IW735_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1043295.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(7034..8563)
                     /gene="ahpF"
                     /locus_tag="IW735_000266"
     CDS             complement(7034..8563)
                     /gene="ahpF"
                     /locus_tag="IW735_000266"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1043296.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8573..9136)
                     /gene="ahpC"
                     /locus_tag="IW735_000267"
     CDS             complement(8573..9136)
                     /gene="ahpC"
                     /locus_tag="IW735_000267"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1043297.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9280..9942)
                     /locus_tag="IW735_000268"
     CDS             complement(9280..9942)
                     /locus_tag="IW735_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1043298.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10126..11772
                     /locus_tag="IW735_000269"
     CDS             10126..11772
                     /locus_tag="IW735_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1043299.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11763..12845
                     /gene="uxuA"
                     /locus_tag="IW735_000270"
     CDS             11763..12845
                     /gene="uxuA"
                     /locus_tag="IW735_000270"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1043300.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(12953..14506)
                     /locus_tag="IW735_000271"
                     /pseudo
     CDS             complement(12953..14506)
                     /locus_tag="IW735_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            14654..15795
                     /locus_tag="IW735_000272"
                     /pseudo
     CDS             14654..15795
                     /locus_tag="IW735_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(15921..17000)
                     /locus_tag="IW735_000273"
     CDS             complement(15921..17000)
                     /locus_tag="IW735_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1043301.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(17103..17543)
                     /locus_tag="IW735_000274"
     CDS             complement(17103..17543)
                     /locus_tag="IW735_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1043302.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(17600..18010)
                     /locus_tag="IW735_000275"
     CDS             complement(17600..18010)
                     /locus_tag="IW735_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1043303.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(18190..18624)
                     /locus_tag="IW735_000276"
     CDS             complement(18190..18624)
                     /locus_tag="IW735_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1043304.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(18724..19572)
                     /locus_tag="IW735_000277"
     CDS             complement(18724..19572)
                     /locus_tag="IW735_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1043305.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            19961..21727
                     /locus_tag="IW735_000278"
     CDS             19961..21727
                     /locus_tag="IW735_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1043306.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            21829..23088
                     /locus_tag="IW735_000279"
     CDS             21829..23088
                     /locus_tag="IW735_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1043307.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            23235..24536
                     /locus_tag="IW735_000280"
     CDS             23235..24536
                     /locus_tag="IW735_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1043308.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            24539..25390
                     /locus_tag="IW735_000281"
     CDS             24539..25390
                     /locus_tag="IW735_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1043309.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            25403..26218
                     /locus_tag="IW735_000282"
     CDS             25403..26218
                     /locus_tag="IW735_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1043310.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            26369..27535
                     /locus_tag="IW735_000283"
     CDS             26369..27535
                     /locus_tag="IW735_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1043311.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            27765..28412
                     /locus_tag="IW735_000284"
     CDS             27765..28412
                     /locus_tag="IW735_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1043312.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            28496..29440
                     /locus_tag="IW735_000285"
     CDS             28496..29440
                     /locus_tag="IW735_000285"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1043313.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            29575..31026
                     /locus_tag="IW735_000286"
     CDS             29575..31026
                     /locus_tag="IW735_000286"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1043314.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            31047..31883
                     /locus_tag="IW735_000287"
     CDS             31047..31883
                     /locus_tag="IW735_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1043315.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            31883..32542
                     /locus_tag="IW735_000288"
     CDS             31883..32542
                     /locus_tag="IW735_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1043316.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            32668..33357
                     /locus_tag="IW735_000289"
     CDS             32668..33357
                     /locus_tag="IW735_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043317.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            33363..34319
                     /locus_tag="IW735_000290"
     CDS             33363..34319
                     /locus_tag="IW735_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1043318.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            34363..34875
                     /locus_tag="IW735_000291"
     CDS             34363..34875
                     /locus_tag="IW735_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1043319.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(35131..35679)
                     /locus_tag="IW735_000292"
     CDS             complement(35131..35679)
                     /locus_tag="IW735_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1043320.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(35768..36948)
                     /locus_tag="IW735_000293"
                     /pseudo
     CDS             complement(35768..36948)
                     /locus_tag="IW735_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(37184..38692)
                     /locus_tag="IW735_000294"
     CDS             complement(37184..38692)
                     /locus_tag="IW735_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1043321.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            38851..39042
                     /locus_tag="IW735_000295"
     CDS             38851..39042
                     /locus_tag="IW735_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043322.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            39245..40162
                     /locus_tag="IW735_000296"
     CDS             39245..40162
                     /locus_tag="IW735_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1043323.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            40155..41228
                     /locus_tag="IW735_000297"
     CDS             40155..41228
                     /locus_tag="IW735_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043324.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            41376..41894
                     /locus_tag="IW735_000298"
     CDS             41376..41894
                     /locus_tag="IW735_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043325.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            41875..42267
                     /locus_tag="IW735_000299"
     CDS             41875..42267
                     /locus_tag="IW735_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1043326.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            42343..43278
                     /locus_tag="IW735_000300"
     CDS             42343..43278
                     /locus_tag="IW735_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1043327.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            43411..44190
                     /locus_tag="IW735_000301"
     CDS             43411..44190
                     /locus_tag="IW735_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1043328.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            44183..44500
                     /locus_tag="IW735_000302"
     CDS             44183..44500
                     /locus_tag="IW735_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1043329.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            44744..46354
                     /locus_tag="IW735_000303"
     CDS             44744..46354
                     /locus_tag="IW735_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1043330.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            46511..47359
                     /locus_tag="IW735_000304"
     CDS             46511..47359
                     /locus_tag="IW735_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1043331.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            47590..48069
                     /locus_tag="IW735_000305"
     CDS             47590..48069
                     /locus_tag="IW735_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043332.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            48102..50330
                     /locus_tag="IW735_000306"
     CDS             48102..50330
                     /locus_tag="IW735_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1043333.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            50459..52102
                     /locus_tag="IW735_000307"
     CDS             50459..52102
                     /locus_tag="IW735_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1043334.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(52175..52720)
                     /locus_tag="IW735_000308"
     CDS             complement(52175..52720)
                     /locus_tag="IW735_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1043335.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(52743..53228)
                     /locus_tag="IW735_000309"
     CDS             complement(52743..53228)
                     /locus_tag="IW735_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1043336.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(53218..54030)
                     /locus_tag="IW735_000310"
     CDS             complement(53218..54030)
                     /locus_tag="IW735_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1043337.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(54169..>55278)
                     /locus_tag="IW735_000311"
     CDS             complement(54169..>55278)
                     /locus_tag="IW735_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1043338.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        18312 a        10020 c        10704 g        16242 t
ORIGIN      
        1 atattaaaga ttgttaaatt tcactatttc atttcaaatt ccattgccta atcattagga
       61 aaagtctatc atgaactcgt aaatagaata tagtaataac cttgttaggt gaggctccta
      121 tacaaacata ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg
      181 ttggattaag gctttttcca atagctaaga atgttttctt tacgttgtat agtgctaaaa
      241 ctcggacgat gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc
      301 tttatatttt tgtgactgac ttatttgatg agacaaactc gctcctcacc tgacaagtgg
      361 attgctgtgt gaggaggaga tgcggtgaaa aaagatcgat tggtggattt tgggtcctta
      421 tttgactttg tccatgatcg gtcttttaga agtatacagt gccagttctt atcgtttatt
      481 gcaagccgat gaaaatacaa aaagtctatt attgcgtcaa ctaattttca tatttttgag
      541 ttggagcgtg atcttcttag ctcgttcagt caaactacac tatttacttc accctaaaat
      601 agcaggatac ggtttagcct tatcgatttt ctttttagta ttagtaagaa tagggatatt
      661 cggtgtcact gtcaacggcg cacaacgttg gatctctctg tttggcattc aattccagcc
      721 ttctgaactg gcaaatcttt ttttgatttt ttatttaagc tggttttttc gtgacggaaa
      781 tagtagccca aaagatctaa aaaaaccatt cctgattaca gtaggtataa cttttctgat
      841 tttatttcag ccaaagattg ctggagcatt gatgatcctt tcgattgcgt gggtcatatt
      901 ttgggcagcg gcggttccat ttaaaaaagg gatctatcta atcgttactt tttctgcatt
      961 gctgattgga gcagcaggcg gggtattata tttaggaaat aaaggttggc ttccacagat
     1021 gtttaatcat gcttatgaaa ggatcgcgac tttaagagat tcttttatcg atagtcatgg
     1081 tgcgggctat caaatgaccc attcgtttta tgcactatat aatggtggca tatttgggcg
     1141 gggattagga aatagtatca caaaaaaagg gtatcttcca gagacagaaa cagattttat
     1201 tttttcgatt attacagaag agcttgggct aatcggtgca ttgtgtgtgc ttttcttatt
     1261 gttctcatta tgcatgagaa tcttttgttt aagcagtcgc tgtaaaaatc agcaagctgg
     1321 attgtttctt ttaggattcg gaactttgct attcgttcag acgataatga acgtgggaag
     1381 tatcgcgggg ctgatgccaa tgactggtgt gccattgcca tttgttagct acggaggaac
     1441 aagttatctg atcctttctt taggaatcgg aatcacgttg aatatttctt caaagataca
     1501 ggccgaagag ttggcgctct atcgacctga aaaacaataa agagaaactg agaaactctc
     1561 agtttgaatt atcataaagt acaatctttt atttttcaag cgagaggttc ttttagaaag
     1621 aggaattcgc aatgaaagta tctcaaaaaa tcatattatc tattttgtta ctgcttacat
     1681 tagtagttgg ctatttttcg attgagttta tccatggatt ttcgtctgca aaacagagtt
     1741 caactgtgaa aaaggtagag ccgaaaagta tccctaccac actaaatgtg gctttgattg
     1801 gttcagatgc tcgttcgaaa gacgaaaacg gtcgctcgga ttcacttctg gttgcacaat
     1861 acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca tatgtcgata
     1921 ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga gttgatttat
     1981 taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca agtatcacat
     2041 ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag atcgatgcag
     2101 aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg atggatggta
     2161 atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc gggaggatca
     2221 gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta acttcaatat
     2281 tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac cttccagaaa
     2341 gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat aaacaggtat
     2401 aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa agaagtgaca
     2461 agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta atagcagctg
     2521 cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta gaagctggag
     2581 aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac aaagctgcag
     2641 aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag atcttagata
     2701 aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg aatctaaaag
     2761 tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg aatacctcat
     2821 taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat ggtcaaacca
     2881 cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac aaagtaagcc
     2941 tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa ccattagcaa
     3001 caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat ggaggcgaaa
     3061 aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat gctatcaatc
     3121 aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa atcattgatg
     3181 gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc agatattatc
     3241 ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca gcagaagata
     3301 ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt ttggagatgg
     3361 cttttgataa ggatcttcgt gggacaacag gtgggaaatt aagcattaca gatgcagacg
     3421 gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat atcaaattga
     3481 caatcgatgc gaaggcacaa aaaacagctt ttgacagtct aggaggaaaa gctggatcaa
     3541 ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct ccaagctatg
     3601 atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat gaagaaaatc
     3661 ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct acgtttaaaa
     3721 tgattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa gtgttgacga
     3781 tcaacgggct taaatggcaa aaagacagtt cttggggatc ttatcaagca acgcgtgtca
     3841 gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat aatatatatg
     3901 cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg tacaggctta gataaattca
     3961 tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt tctaatgaag
     4021 atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc gaacttctaa
     4081 ttactcctat ccagcaagca gcaatgtatt ctgtttttac caacaatggc acacttgtct
     4141 atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc agcgaaacag
     4201 cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat ggtacagcac
     4261 attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg gaaatcaaag
     4321 aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat cttgataacc
     4381 aaggttatat gatggtcagc atgttggaaa ataaagaaga tgatgattca gcaaccaaac
     4441 gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt gctttttaag
     4501 tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa cggttttcac
     4561 gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt ttttgtaaaa
     4621 tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac taaaggtgaa
     4681 tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat caaagccatt
     4741 tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga aaaaccggca
     4801 atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag cgtcaatata
     4861 gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc tacactgaca
     4921 aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta tacttcttct
     4981 ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac agagctaagc
     5041 ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac gatgataata
     5101 gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat ccatttgact
     5161 aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg taaagaagca
     5221 ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa aacgactaaa
     5281 atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt cactacgttt
     5341 agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat aatcccaaag
     5401 aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc aaaaacaacc
     5461 gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt tttcaaaaga
     5521 gataaatcac tttccagacc agctaaaaac attaataaga tcacaccaat ctccgaaaag
     5581 tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag caaaacacca
     5641 acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg tgagccaatc
     5701 gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat cattcctttg
     5761 tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg aaaatatact
     5821 cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat acggtctatt
     5881 ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa attgataatc
     5941 tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag catcaaactt
     6001 ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact actataaaca
     6061 ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct gttgatgata
     6121 acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa agcatagacg
     6181 atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca agaacctgtt
     6241 ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac aagataggcg
     6301 gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat cgcaatccca
     6361 gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg tttaatcgtc
     6421 tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca ttttaccaaa
     6481 ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa cttctgagga
     6541 tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa taacgaattt
     6601 ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca cctgtaatca
     6661 aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa gtcacttgtt
     6721 tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa atgatttcat
     6781 ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata ggataaacgc
     6841 ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt tctttttttc
     6901 aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt attcaaaaaa
     6961 acaaaaagga ttgtgacaaa attcttgtca ccatcctttt ttccgctctc tgacttttta
     7021 caatcttcat agctcaagtg cgcatcagat aatcgaatgc tcctaaagct gcagtagctc
     7081 ctgaacccat tgaaacaatg atttgtttgt atggactatc tgtacaatca ccagctgcga
     7141 aaacgccggg gatattcgtt gcgccatttt tctcggtcac tatttcattt cttggattca
     7201 tttcgatcgt tccatctagc caatccgtat tagggagtaa gccgactaga atgaatactc
     7261 ctgaaacagc tagtgtatgc atctccatcg tacggcgatc gatataggag agagattcta
     7321 cctgattcgt accgctgatt tctttcgtcg ctgcatgttt gataacagtg acattcgcaa
     7381 gagagactag tttatcctgt aagatttgat ctgcttttaa ttctggtaag aattctagaa
     7441 catatacatg ttttgctaac ccagccaaat cgattgctgc ttcaacacca gaattaccac
     7501 caccgataac agcaatctct tttcctttaa ataaagggcc gtcacagtgt gggcaataag
     7561 caattccttt atttttaaac tctttttcgc ctggtacatt gatagaacgc cagtgcgcac
     7621 ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc gttagctaag ccaatattaa
     7681 taaaatgttg ctctttattt aaggaaacgg cctgttgatt catcataatg tcgatagggt
     7741 aactacgcac atgactttct acttgctgca tcaattttgg accttctgtg taagaggtac
     7801 caatcatatt ttcaatccct agtgtttcaa cgacttgtcc gccgaaagaa tcagcgacaa
     7861 gtcctgtacg gattccttta cgtgctgcat agatagctgc actactagct gctggtccac
     7921 cgccaatcac taatacatca taaggttctt tttcaaacaa ctcttcttca atcaaagggc
     7981 cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc gctactaaat tcttctccat
     8041 ttaaaaacac agttggcact gccatgattt ttttatcatc gatttctttt tggaacatcc
     8101 caccttcaat catcgtatga gtaatatttg gatttaatac agctaatata ttcaatgcct
     8161 gtacaacgtc ggggcaatta tgacaagtca agctaacata actgtcaaaa tgcaatgttt
     8221 gtgttatttt tttgatttta agactcaaag attcactgat tttcggtgcc cgtccactaa
     8281 tttgtaacat agccagtaaa aaagaggaaa attcatgacc gagggggata ccagcaaaaa
     8341 cgattccact ttctttgcca actggattga tagaaaaact aggtgtgcgc atcgaatctc
     8401 ttttttccca agagatttta ggagacattt ctgctatttc cttcgtaaat tcagctagtt
     8461 tttgactatt ttcactatta tccaaactaa cagaaaaaac aataggagat tcaatcagtg
     8521 ctaaatattg ttcgagattt tgtttagttt ctttgtctaa catatccacc acctaaatct
     8581 ttccaacaag atcaaaactt ggtttcaatg tttcgccact ttctttccat tttgccggac
     8641 aaacttcacc tgggtgggta cgtacatatt gagcagcgcg aatcttatca atcagtgcac
     8701 tagcgtcacg accgattcca tctgcattga tttccatagc ttgaacgatt ccgtctggat
     8761 caataataaa tgttcctcgc tgagctaatc cttgctcttc atccaaaaca tcaaaaatac
     8821 gagaaatctg atgagacgga tcaccgatca tcacgtattc gattttaccg atagcatctg
     8881 atgtatcatg ccatgctttg tgagtaaagt gggtatcggt agagcaagaa aagacttcaa
     8941 cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc ttctaattca gtaggacaaa
     9001 caaaagtaaa atcagctgga tagaagcaaa gaatactcca gttccccata agatcttttt
     9061 cactgacttc taaaaattct ccttgatgat aagcatgtgc attaaattct tcgatttttt
     9121 tcccaatcaa tgacatagta aaacctccaa taataattat ttatttataa ttattgtaat
     9181 ttagttggag ttgcaaagtc aatggatatt ttgttattta ataaagatta ctcaaaaacg
     9241 aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc tactgctgga aatactcggg
     9301 ataacgttga gtaacagttt cctgttcttt cagcatcatg tgtaagtgca atcccgtttg
     9361 aaatcgagct tcttctggtt ttttatcaac taaagccttg agtaaatcat aatgttgttc
     9421 catgatcgtt tcccatggaa gcccctgaat ggtcactcgt aaccagcgga aacgttctaa
     9481 atgggtatta tggtcatcta accagttcca aacttctttt cttcctgcaa tttcgaaaca
     9541 agcttcatga aataaattat ctgcacggaa aaaagcgcgt tcgtcattta ttttgactgc
     9601 tttttcttgt tcttctaaaa tcgtttctag cactattcgt gtttgtgtgt ccaatttgga
     9661 acaacattca atcatgattt gctcttccaa ctgttctcgg acaaagcgag cattttctgc
     9721 agaaacaaga ttaattttgg aaacatacgt accactttga ggtatcgtat acacgagttc
     9781 ttgctgacgt agttgaatca atgcttcgcg aatgggagtc cggccgatag caagatgttc
     9841 ctccagtgcc ttttcagata ttttccttcc aggttctaag tcagcataga taatttgttt
     9901 acgaatcgct tgataagctt gtgattgcaa gctgcttttc atctttattc ctccaaaatc
     9961 atttctatga acgtagtata acagaagagg tctgaaaaac aatctggtat gtcacattaa
    10021 ctagtaaatt tgtataaata gtaaaatttt ggttgacata gcgattttta ttgcataaaa
    10081 ttcacttgaa tacaaaaact aatatgttag tttaggaggg gaaaaatgat tgatttaaac
    10141 aactggagga actttatccc tgaattatct caaaaagaag tagaaatacc tcattttgac
    10201 cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct ggatccattt cggtggcgga
    10261 aatctttatc gaggatttca tgcagaaatc gctcaacgtt tagcggatct aggcgaattg
    10321 tccactggtg tcattgtttg tgaaacattc gatgaagaaa taattgaaag ggcttaccaa
    10381 gattttgatg atttgattct tgaagtggtc atgccaagca gtggagagtt cgtcaaacgt
    10441 atattagctg cgacagctga cagtctgtat tgtcatccag attgttctga acactttgat
    10501 aagatgattc gacactttga aaatcctagt ttacaattag ttacttttgc tattactgaa
    10561 aaaggttatc gattgaaaga tacaaataat aaatatatag gaccgattgc agtcgatata
    10621 aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgtgct actttataaa
    10681 cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt ttcacaaaac
    10741 ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa ggaaggttat
    10801 gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt tccatggacg
    10861 atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt ggcaaaagag
    10921 ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc tccatttgtc
    10981 aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg acgtccgaaa
    11041 ttcgaaaaag ctggggtatt cttgactgat cgtgcaacgg tagataaagc agatacgatg
    11101 aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg ttgtttactg
    11161 gggtatagga gcatagctga ggagatgaaa gatccagatt tatcgctgct agtttctgaa
    11221 atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga tccaaagatc
    11281 tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctaa tatgcctcag
    11341 cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac gattaaaaaa
    11401 tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt gactctcgct
    11461 gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat cccgagtcca
    11521 gacccactat tgacagacct tcagcataag ctttccgata ttcaactggg tacgcaagat
    11581 acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat ttttggtgta
    11641 gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa gatggtagca
    11701 ggaccaaatg ccgtacgaaa aacattacag gattacttac aagaacatgg aggaaattta
    11761 tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc ttagatgaaa
    11821 tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata ccagtaggag
    11881 aggtatggcc tagggagagg atcaaagaac taaaagaaga aattgagtct cgtgggttaa
    11941 cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga ttaccttccc
    12001 gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa tatggaatag
    12061 aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac cttgactacc
    12121 gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca gatgatccaa
    12181 gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tggattgccg ggatgggaac
    12241 ctgagcgctt agctcctatc aaatccttgc ttgaagccta tgcatcagta gacgaagaaa
    12301 aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt gaagaggtag
    12361 gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga ttagctcgaa
    12421 tcgtaaagaa tcgagaagat ttagattggt tgtgtcgcat agtagacagc ccttataacg
    12481 gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta tatgggattt
    12541 tagcagaatt ttgccaaaga gatcgaatcc catttgcaca tgttcgaaat attaaatttt
    12601 tagctaatcg agatttctat gaggcaccac ataaatccga atacggttca ttagacatgt
    12661 accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt cctgatcatg
    12721 gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat cgagcattag
    12781 gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa agtaaagata
    12841 attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa attcttgata
    12901 cataaatccg gaggttgtta ataagcattc ggatttatgt atcttatttg gtctaggaag
    12961 taacttgatt ttcttttttc gattgatcca tgagtgcttc ttcttttaat tcaaagatat
    13021 attctgagat cctcataatc acttttttcc agtcttctgg tgtgatgatt ccttttaaaa
    13081 tatagatttt tttgatatga gaaggcaatt gtttcaaagg gcagtcgctg ataacgatgt
    13141 tgcagttctc cataaaatca ttttgtattt tgatcgattc atttgaaaaa atttgttcta
    13201 tcttaacgat cagatcttta ctgatataaa aatcacgcgt atagttcaca ccaatcacaa
    13261 ttcgcggctg ttttttgtag cgatcataca aatgtactag gtcttcaacc atccattgca
    13321 cattttcctt ttctaaaata actggtaaca agtgttgatt ttgttctctg aaatccatat
    13381 aaaattgttc aatggcggtt tggacttctt tcaatacgct atgttcttga taaagcatga
    13441 aagtaggaag gggcgtattt ggttgattga agttaagaaa tccattataa agtttatttc
    13501 gtgcgaatgc caatgagtaa agaatctttt ctttttcagg tataaacaaa tcaaatacag
    13561 tacaaaggga ctccaccatt tctcttgaat aaagaacatg aggaacgttt gcttttttta
    13621 aaagtttgta ttgctccaag cttgtttctt ccgattcgat tttcgtgata gaaagtcgag
    13681 ctaaaatatt cactaagatg cgttgttcgc gacaaattgg cagattaggt cgcaaaatat
    13741 ctacagagag atcaataaaa aagtcaatat attcatactc tgtgaattct ttttgtacgt
    13801 ctttcacaga agttgcagga aaattctctt tacatatttt taatagaaaa tataatttat
    13861 ctatcatacc gctatttagt cggtcaagta cttctttttt aaaaaaagta ttcatcataa
    13921 aagccactac ttgcggatct tcatcataaa attctgtagc agtacttgaa aagatgttcc
    13981 aataaatatc taatgctgca tattgaattt gtacctcttt tccagaaaaa gactttctgc
    14041 cattattaga aaaggagata gacaaaccgt actatgctaa aaatgcatta atctttttca
    14101 ttttcgtata caaataagag gcactatagt ttgtttgctg actgaaactg ataatcgaat
    14161 ttgtctgaca gctgaacaaa gaagtcaaga tctgataact gactgattcg ttgcagtatc
    14221 ttccaaatag acgataatag cagtaagccg gatcaattcc ttcaaaattc aactttaaaa
    14281 aaggtttttg cttatcaata tgcagttctg ttagattgag acgctcaaag tcttgttcta
    14341 attctttgat gtatagtaaa aaatttgatt cagacatatg gaaatgatct gctaaatatt
    14401 gtgtagtaat aaagaaacga tgttcagata ggaaaagaaa aatatctaat tttgtattgc
    14461 tttttttctt caagataaac gttttaatcg cttcttgagt ttgcatgaaa gaggctcctt
    14521 tttacttaaa ttgttaaaac ttgaaaatat tactgttttt ttcataaatt tagacaaaaa
    14581 aacagtaaac atataggtag aatctctact atcttatttc ttgcatacaa aaaaatcaag
    14641 gagtgagaga gttatgagat attcaaccat cttaatatca acatttgcag ccttaagctt
    14701 atcgcttact ataaatcaga cagtccacac agaggagaca gaaacagcca aatggacaga
    14761 aaccaagtgg gccaacgtca atcgaattac ctttcaggat gaattttcat ccttcaattc
    14821 aggagtcgca aaattaagaa aaggacaaac agcgaccatc aaagcaaaaa ttgactattc
    14881 gggagactta gcacctatac tacaatcagc tgttacattt gagaatgttg atcccacttt
    14941 gtcgattggc tatgacgaga acactttgac tatccaacac aagcaagcga tatttacctt
    15001 caccgttaca ttaaatcagg acttgttaaa accagcgctt tttactgtca aggtatcaga
    15061 tggactaccg aacgatcatc atttaacgcc gtatagtcag cggcagacaa ttgagcaaga
    15121 ttcagccatc gactcaggag gagatcttgt tgaagaacca acagataaac cagaaaatga
    15181 aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac cggaaataga
    15241 acctggagaa gaacccgata cagaaactca accagagcca gacaataaat caaaacctga
    15301 aataacccct ggagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca
    15361 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga
    15421 ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca
    15481 aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa
    15541 aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat
    15601 atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctgt
    15661 cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa agttatactg
    15721 tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag atttaaacaa
    15781 aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat
    15841 atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc gttcatactt
    15901 tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg gttcgggatc
    15961 tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac aatcaagccc
    16021 caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa aatcggcagt
    16081 tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag atggacaatc
    16141 aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata agctatgaaa
    16201 gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa aatgaaaatc
    16261 atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc
    16321 aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa tgtattgaca
    16381 aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact ttttaaaaac
    16441 aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact taagatgaat
    16501 gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag cgcaatcgaa
    16561 gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg gatatattgt
    16621 aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat taataacgga ctggatcatg
    16681 tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata gataaggtaa
    16741 acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac aaatcgctga
    16801 atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa aatggttgta
    16861 atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt gagacggcgt
    16921 attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc caaataacaa
    16981 aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag caaaaagaaa
    17041 aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt catctagcag
    17101 tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt cagatgttgc
    17161 agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg tggaatacct
    17221 tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg taacatttac gattctttca
    17281 tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt tagtaaggga
    17341 gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga agtctttttc
    17401 ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag cggcttgagg
    17461 aggggagtgt agtgtctaat gatttcttta gaggcggcat ataaaggaaa acatacttga
    17521 ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag tgtatcatag
    17581 cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct atatttccag
    17641 ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct tgcgtttctt
    17701 ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag ctcgtttcac
    17761 tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata tttttttgct
    17821 gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca aacaattgtt
    17881 cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat tcgaatgtac
    17941 gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt gatcattttc gtagaataca
    18001 tttttttcat aaaattacca cctttaatta tattttatac aattgaattt tacacaatat
    18061 aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc acatgaaaca
    18121 gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc agagacaaaa aataatctca
    18181 tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa ttacttcacg
    18241 atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg agttagtcac
    18301 accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc gtaatgcttt
    18361 attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat ttggatgttc
    18421 agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga atctcgtacc
    18481 atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct caatcagttg
    18541 tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat ttaatacaaa
    18601 atcgatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga acagttacta
    18661 gattctttta ttataccagc taaagaaaac gcattcaaaa aaatagcgga tttacttacc
    18721 aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta cgctgctgac
    18781 aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg aaaattcatg
    18841 atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc cttttgcctc
    18901 tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt cttccaaata
    18961 atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga tataagtcat
    19021 gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta ttccaaaaat
    19081 aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg aagtattcaa
    19141 gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag aagagttgtt
    19201 tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga cagacaacaa
    19261 ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa aaaccaagct
    19321 tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta gtaccaacat
    19381 aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag cttccagtaa
    19441 tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa acatattgat
    19501 agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata taaattgtct
    19561 cgtttctttc atttattcac ttccttaatt cattaataat actcttatct tattataact
    19621 tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct aaattattat
    19681 gtttcattct tctagaatat acttctacgc caaaaactgt taaaaaactt ttactttaga
    19741 aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac aggaagtaga
    19801 ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg
    19861 tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact
    19921 tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta
    19981 tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct
    20041 tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat
    20101 aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt
    20161 tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca
    20221 tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg
    20281 agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga
    20341 tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag
    20401 atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga
    20461 tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta
    20521 cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa
    20581 tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact
    20641 tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt
    20701 attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa
    20761 atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac
    20821 tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat
    20881 gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta
    20941 ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca
    21001 tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct
    21061 tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat
    21121 gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc
    21181 acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg
    21241 aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa
    21301 caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat
    21361 ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat
    21421 gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc
    21481 tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa
    21541 tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca tctatgttta
    21601 ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca
    21661 aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat
    21721 gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca
    21781 ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga
    21841 agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct
    21901 tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt
    21961 tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa
    22021 aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg
    22081 ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt
    22141 gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat
    22201 gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc
    22261 gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa
    22321 gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat
    22381 acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac
    22441 ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca
    22501 ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga
    22561 atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact
    22621 gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat
    22681 ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga
    22741 aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat
    22801 tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca
    22861 aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt
    22921 gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca
    22981 ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca
    23041 gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa
    23101 ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg
    23161 gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta
    23221 gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc
    23281 tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg
    23341 tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat
    23401 tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga
    23461 ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc
    23521 ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga
    23581 tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg
    23641 cttatcttct catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat
    23701 ttactaatta tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta
    23761 aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt
    23821 cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca
    23881 cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct
    23941 tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg
    24001 atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag
    24061 atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag
    24121 tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga
    24181 tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt
    24241 caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata
    24301 cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg
    24361 taggcggagg agctggttct actgatattc ttatttcctg gatctataaa cttacgactg
    24421 gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta
    24481 tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat
    24541 gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt
    24601 ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc
    24661 cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg
    24721 aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat
    24781 catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta
    24841 tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat
    24901 ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct
    24961 cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg
    25021 gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga
    25081 tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct
    25141 cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt
    25201 cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga
    25261 tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat
    25321 ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac
    25381 aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg
    25441 tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat
    25501 atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat
    25561 ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac
    25621 ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc aacaaacgat
    25681 agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca
    25741 tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg
    25801 tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc
    25861 cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat
    25921 tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt
    25981 cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt
    26041 caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt
    26101 tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt
    26161 cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca
    26221 agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga
    26281 aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa
    26341 tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt
    26401 ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata
    26461 cggagatacg gacactccta cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa
    26521 agagattaca agtaacgcat ctttggcact aggagaagcc tatatggatc atcgaatcga
    26581 gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt
    26641 acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa
    26701 caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga
    26761 tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc
    26821 acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt
    26881 gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt
    26941 caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa
    27001 agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg
    27061 cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct
    27121 tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca
    27181 tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc
    27241 tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca
    27301 actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa
    27361 gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat
    27421 gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat
    27481 tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag
    27541 gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg ctccagaagt
    27601 gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt
    27661 ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta
    27721 tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta
    27781 tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag
    27841 gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct
    27901 caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc
    27961 agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag
    28021 atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag
    28081 aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg
    28141 agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag
    28201 atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca
    28261 aagtaggaag cggtacggca acagattggg atttggataa agatttaggt actacttatt
    28321 gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag
    28381 gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt
    28441 tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag
    28501 tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt
    28561 ccactgcatg actacccagg aattagatga actggctcat tcagaagcag gagcatttat
    28621 cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt
    28681 tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga
    28741 gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat
    28801 tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt
    28861 taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc
    28921 ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc
    28981 gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat
    29041 cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta
    29101 tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg
    29161 cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa
    29221 accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca
    29281 tctgctatgt ggcgcaagca tgttacagat tggtaccgag cttcataaag aagggccgga
    29341 aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc
    29401 aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga
    29461 ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc
    29521 attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat
    29581 gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt
    29641 acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt
    29701 gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg
    29761 gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg gctagctact
    29821 tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta tgtgcggcaa
    29881 gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt tggtttcaaa
    29941 aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct tgaatgtaca
    30001 ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt tcctttagct
    30061 ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg attgattggg
    30121 ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg atggcaagga
    30181 gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt attttatggg
    30241 atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg gcaaacacat
    30301 ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca ctcacagatt
    30361 agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat
    30421 gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc ttattatcag
    30481 atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat ccagcgagct
    30541 gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg tcagaagctc
    30601 tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat tgtcatggaa
    30661 aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac cgcccctaaa
    30721 atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat cagtgattgt
    30781 tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt gaagatcaca
    30841 ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc tactcatttg
    30901 acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag agaagatttg
    30961 ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc aaatccatgg
    31021 aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct ctaataacta
    31081 gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa gataacagct
    31141 ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca ctgtttcccg
    31201 catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg gcaaaagagg
    31261 tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat ggacaaatca
    31321 cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc acaaaagaaa
    31381 gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt atgatcgtac
    31441 gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa aaagcagctg
    31501 gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa ttagtcacat
    31561 atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga acagatgtta
    31621 ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat atccctgtaa
    31681 aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa
    31741 atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa gatgggacga
    31801 caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga
    31861 tggctgagtc ggtgagtgag tgatgtactt tcctctaatt gatttttccc tcatgtggac
    31921 ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct tcgtgtcgtt
    31981 tcttttagga aatctggtag ggttgctttt gacagtttta ggtcttttgg attggctgcc
    32041 tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg cactagtcct
    32101 gctgtttctg ttatattttg ggttacctta tcaattaagt gccttaacgg cttcagtcat
    32161 ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat cactagcagg
    32221 cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt
    32281 gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc
    32341 tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca
    32401 aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc
    32461 actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg
    32521 ttctatgcat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct
    32581 attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa
    32641 tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc
    32701 tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct
    32761 gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa
    32821 tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca
    32881 tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact
    32941 gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg
    33001 cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg
    33061 gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag
    33121 caaatggagc gaggcgtaaa atattttcca acagatcaat tgtacaaaga attgaaaaga
    33181 cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa
    33241 atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat
    33301 gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa
    33361 atatgtacta tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac
    33421 tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg
    33481 ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatcgaatc gctgtgatcc
    33541 aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca
    33601 aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac
    33661 tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg
    33721 tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact agtcgctttc
    33781 gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg
    33841 ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg
    33901 cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag
    33961 ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac
    34021 gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc
    34081 ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta
    34141 cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag
    34201 tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc
    34261 caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat
    34321 tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca
    34381 aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct
    34441 tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac
    34501 gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct
    34561 gcattagtct caagcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca
    34621 actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa
    34681 ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat
    34741 atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga
    34801 ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa
    34861 ataatcatca attgactata aaatcatctg agcaaagata aagacattaa cactactgaa
    34921 aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag
    34981 aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata
    35041 gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgacctctc
    35101 ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc
    35161 tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga tagctttcaa
    35221 acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa tctgttcaga
    35281 tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg taataaagat
    35341 aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac
    35401 ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt
    35461 atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt
    35521 attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt
    35581 cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc
    35641 agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat
    35701 tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt
    35761 ttgtatatta taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg
    35821 ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct
    35881 tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag
    35941 atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag
    36001 caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt
    36061 tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga
    36121 aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac
    36181 cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca
    36241 acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt
    36301 ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa
    36361 gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag
    36421 aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta
    36481 attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat
    36541 tataagattc caattgccca tcccgttgat tttacttaat cctagaccaa cagctttcat
    36601 aaataatgga agaatcttga tatagcgtag aataccgata atagcagaaa taaatacaat
    36661 tggcatcaat acggaaagaa agaaaggtgc acttccttta tttgctacac cggcaaaaac
    36721 aaagtcaaca ccttgcccag caaaagcaag aagatgatca aacatttttg ccaatacaga
    36781 aacaaccgct gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac
    36841 gaacatcaca attagtggtt tcaatttgat tgcttttttg ttactgctga tcaaaaaagc
    36901 aagccctaat gtgacaaata aaccaataat acctaataaa taactcaaat aatcactcct
    36961 attccaaatt ccgttttatt ttacctgttt tgcaaataaa atactaatct ttagtgaaat
    37021 tttcattact tttttagagt gaaacgcatt cattttccat tagaaaataa agcgaacaaa
    37081 ataaaaggtt taaaaagtgt aacgtcttag taaagtctat aaaaaaacga aactatcgat
    37141 taagctaata gtttcgtttt caacatttat aagtaaactt tttctattgg taatatagat
    37201 tttcggaagg gtagacaggt gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt
    37261 catcattttt attgaccatc acagtagagt gggcttgtgc tccctcaagt tcagctagtt
    37321 tatcataagc aacttgagcg attggattcg ttactgcact gattgataaa gcgatcaaga
    37381 cttcattgat gcttagtgca tccaaagagg aatgaagtgt ctttgatttc aatccttgta
    37441 ttgtttcaag aactaatggt gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt
    37501 ttactgcatt taagataact gccgctgacg catccatcaa tgaacttgta cgcccggtaa
    37561 tgatgacttc atctggtaat tcgattgcaa tcacagcaga aggatctttt gtaggacttt
    37621 gcgcttgaat cttatctgca tagtcatggg cagggactac cacgtttcgg tcttcaggac
    37681 gtaaaccgac ttcttccata atcagtttga ttcgattaac tgcttcttcg tcgatcaatc
    37741 cttttttgaa gtcgcattct gtttggaaat aacgacggat gatttcttgt ttagaggctt
    37801 cttggacaac ggtatcatcc acgatcccaa atcctacacg attcacaccc atgtcagtag
    37861 gagattgata aacagattct ttaccagtaa ttttttcaat aatacgtttg attacaggaa
    37921 atgtctcaac atcccgatta tagttcacag ctactttatt gtaggcatca aagtggaagg
    37981 aatcgatcat attgacatct tttagatcga ctgtggctgc ttcataagca atgttcagcg
    38041 gatgtttcaa cggaacattc catactggga aagtttcgaa cttggagtaa ccagctgcat
    38101 gtcctttttg gctttcatga tacagttggt tcaaggaagt agcgagcttt ccacttcctg
    38161 ggccaggagc tgtcactaca acgattggtt tagttgtttc aatatactca ttttttccaa
    38221 aaccatcttc tgagacgatt ttttctacgt tgactggata gtcttcgatt tctgagtgtt
    38281 tatagacctt gatgtttcgt ctttctagtt tattgataaa gactttcgtg gcaggctgtc
    38341 cactatagcg agtgataacg acactgttga ttaataagcc ataaccacgt aattcatcga
    38401 ttaaccgcaa gatatccata tcataagtaa tcccataatc accacggatc ttattacgtt
    38461 caatatctcc agcatagacg caaatcagta tttcagcttg atctttcaat ttttgcagca
    38521 atttgatttt cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa
    38581 ctagcttccc accaaattct aaatataatt tgtcatagtg gttcactcgt tccaaaatat
    38641 acgctgactg ctcttcaata tatttttctg aatcaaaacc aatttttttc actaggttcc
    38701 ctccttaagt actaaagcat tttttcattt ttttgaggat tcgtcaactg gaagagcgaa
    38761 attcctcaaa aaaacaaaga aaatggttta aaccaacttg tctagatagt tattcattgg
    38821 tatgatagtt tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca
    38881 tataaacact atatccgttc gatttttacc ggttatgaaa gaatgcttga agatcccgaa
    38941 ttggaacaat tgacgtttac ttatctaaac gaagaaacgc agctaactcg tgatgagcat
    39001 cagcgcattc atttcacaac aagagatctg cctagtaaat aaaaaataag ataaaggtca
    39061 tcggattcga ttctgtccta tcagatggaa agactagttg atcgaaaaaa aggctgtacc
    39121 aaattttggt gcagcctgtt ttatggagca ttgcccgctc ttttttataa caatttttta
    39181 cagaaaatgg tactattgaa gtattaataa gtgaaaaaca gatgagtaag aggaggcaag
    39241 tagtatgaaa gagccagata caaatgcaaa ttatgagcca gacgaactag aggagacgaa
    39301 tgcggatcag gatacaggac aagaatcgtc ttctttttac gatatcaaag aaaaagcaag
    39361 catgaatctt tcccaattat tagaagactt acaggctttt gaaaaagctg tccgcgaaga
    39421 agatatccct gagatctacc gtttatacaa tggccgattg aatgaagaat taaaaagaag
    39481 ctcaaatgaa aatcacgaga tcgatcaatt gctgatgcgt aagatccatg atcgtttttt
    39541 acaggaattt ccttttatgg aacaagtaga aaacatctcg ccaacgatga gctattacaa
    39601 aatcggcact tattaccatg atcggcccac ggtcggtatt gatgcaagtc tgccagaaat
    39661 ttttgttttg cctaagatcg atgaagagtg ggagaaatat tcccaaccgg agtcaacgga
    39721 ctatgacaaa aaaatcaatg agctagacgc aaaggtgatc actgctcaga cagaaattga
    39781 acgtttagat gaacaaataa aagaaatcaa tcgacaaatg actgatttga acgacaataa
    39841 aggattcttg aatcgtaaaa aaatcgagga agaaattcaa gaattagaga agaagaagca
    39901 agtactatct aatgaaaaat taggctggct tccttatatt gagacgcctg aaacaatcca
    39961 gcagcaaaaa gaagcattga agcaagaagc aagagcagac cagcttcgtg cagccatcgt
    40021 agaaaaagaa caaaggcaaa tcagacgcta tttcggtagc aaagagggat tcggtcaagc
    40081 aatccatgaa ttcttgatga attatcttgg caatgagaat cctaataatc aatctaatga
    40141 aggaggttcc gagtatgaat gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa
    40201 cagaagatca tgaagaaacg aacacttcat tggaagaaca aacaagtaat cctctggatg
    40261 acacagaaga aatccctatg ctagaggaaa caaaaataca cgaaaaagaa aaagtaactc
    40321 cgacgaatac gaatactgca gaaaacaccc atgcttacga aataactgaa aatcccgggt
    40381 atttagaagg aaaagagcaa gatcatcaag atatcgtcag tttgattgaa acagcggaag
    40441 cagaattagc ggatttacgt atgagaaaag atttgatcga atcagatttt ccacaattat
    40501 tagaatcata tgatcgtttt atattgaatg aagaaccaat ctccacgatc ggaaagacaa
    40561 gcctgatcga atacttcacg tatgaattac tgaaaccgtt gaatgatatt ggtctggaac
    40621 ggtcaaattc agcttatgac acatggaaaa caagacattt ttcgatcaat tatacgttga
    40681 atggacaaaa cgaattagtc ttttcctttg tattaccgac catcgatcaa cgataccaag
    40741 tggaatcgat gagtctccta aacgtatcac cagagacaat ggaaatcaat gtccaagatg
    40801 accgggtgct ttctttgatc cgacactggt cagtggatcg agtattctct gcagggcaga
    40861 tcacgatatt caatcacaaa ttgaatcaat tattagccca tgcaagaaca cttggtttta
    40921 ccgttaatca gacattacta gacaatacga agccacttcg tttgaatctt cagagcgaat
    40981 ttgaattgac gaatgaagta ttggatgata tttttatcgt ggcaatgaaa aatccatcct
    41041 acgatatgga aaagatcaac gaaactactt atcaagtacg attagataaa ggacagtctt
    41101 taacgataac aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct ggagactacc
    41161 cacgatctgt cattgatttc tttatcaatt acgaattctt ggtcccatta atggtgagaa
    41221 agtcctgatt ggataagttg gattaccaga tgtcattcag ataattctgt tccaaaggac
    41281 ttgcggcagt tatccagaat aattgtcaca agtcttttct gtatgtacta aatggacgtg
    41341 aataacttgc tgatgaagga gggatttcat taggaatgtc cacaaataaa atagcacagc
    41401 ttagcttatt gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt attccgaatt
    41461 tccagccaat gactgctatt tttttattta tagtacttta cttaagctta aaagatgcgt
    41521 taattgtcat gagtctttct attgcgatca gcagttttta ttttggtgtt ggaccatggg
    41581 tcatcggcca atggatcagc tatactgttg ttctgagttt gttttcaatc gtctgcttga
    41641 gaaaaacagt gcaaaggaat ctatggttaa agggagtctg tttttttctg gcgggcatgg
    41701 tttacggaat aggtatgacc gtttttgata cgctgttata tcgtttgcct caaccttgga
    41761 tctattacct tcaaggggtg tcgtttgatt tgatgcacag tataggcaat gttgtgttct
    41821 ttcttgtttt tctgccagtc gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa
    41881 aaaataattt atagtttgct tgcaatgagt gtcagtatac tcattttgac aagttgcagt
    41941 acaacctata cacaatcggc ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca
    42001 acaatcacgt tacaagaaaa tggtcaaaac ttttcggaaa aagaagtgtt cttcaaaaaa
    42061 ggcgatgatt tactgagtat tttgaaacga aattttgaag tcaaagaaga taacggcttc
    42121 atcacttcgc ttgaagggca ctcacaggat gagaaaaatc aactctattg gatgtttact
    42181 gtggacggga aaagtgctac tactggtgcc aaagagatca aattgaatga tggacaatcg
    42241 gtcattttca atttgtctaa actataagtt tcataaaaaa ctgttaaagg gcgaatattg
    42301 ccataattca gtgagttcag ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa
    42361 agcggaatgt tttccgtaat tgctgctcta ggggctaata ttctagttgc tatcagcaaa
    42421 tttgtcggct atgcaatcag tggcagtgcc gccatgctaa acgaaagcat ccacagtatc
    42481 gttgattgca gcaatcagat tttgctgtta ttcggtgata aacaagccaa taaaggacag
    42541 agcgagttgc atcaatttgg tgaaggacga gcaaaatact ttttcagtac aatcgtcgca
    42601 atgatgcttt tcttcggcgg tggggcactt ggggtgatgg aagcttttga aaagttgacg
    42661 catccatccc atgaagtggg taatccgatc attgttatag caatactgtt atttggtttg
    42721 gtaatcgaag gaagttcgtt acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa
    42781 aaactttcca cgatgcgctt tttgcgcgaa agtcgacaca gtgaaatact gattattttt
    42841 acggaagact tgtgtgctgt aatcggtttg gtcttggctt tagcaggaac aatattgact
    42901 tatgcaaccg ggattgctgc atttgatgcg atcagcggat tgctgatcgg tttcttgctg
    42961 atgggagccg ccattttcct agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg
    43021 acagcaagcg atttatcaaa aatcaagtta gcttttgaac gtcctgagat cagtcggctg
    43081 atcgatataa aaacagttca tttaagtcca acggagattt tagttgcagc aaaagcagat
    43141 atcgttggta acaaagagaa tgaagcttac tcggtcatca atgatattga aaaaaatatg
    43201 cgtcagtctc ttcctgacaa gaagatgtat atatacattg aaacagataa atatgaccca
    43261 aattattctc gtaaataaaa cgtttattca tgaattgaag caaaaatgag aaaaatataa
    43321 tcttgttttt aaataaaacg gctacaattt agcgaattta tgttacaatg agtccgctgt
    43381 ttatttgttg taattaaagg agatatttta atgaacaaaa agaaaagtgc gccatggctt
    43441 caagctttgc atgcagtgat gccactttgt ataagctata taccagtagg attagcttgc
    43501 ggagtattgt tgcaaaaagt tggatttgat cctctcttat caggactgtt atcgattctg
    43561 gtcttttccg gtggcgcgca gtttttagtt gcatctatgc ttacgacgca agcatcattt
    43621 gcaacgacat tattgatggt tttcttttta gaattacgct atacattatt aggagcaagt
    43681 cttgccggtt ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg
    43741 aatgatgaaa actatgcggt gaactactta aagttttcaa ctgacccttc ctggaataaa
    43801 agaaaggctt tatatgtcaa ttggttctca atgactgcat gggccggtag caatatgatc
    43861 ggtaattttt tcggctcggt gattcgagta gatgctgacc ttgtccattt tgctttgacg
    43921 gccatgttca ttttcatgtt tatcatgcag atgaaaagcg cactactgat ctttacaggt
    43981 ttattctcag gcgtattagg ggtcgtattc atggtattat tccaaaatac tttcggattg
    44041 attgcagcaa cagtcattgc ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa
    44101 gaaaaagcta agaaactaag agaccgcggt aaagatgtca atgagccgct cttcaacttc
    44161 ccggaacagg aggccaatca ccatgactaa actatatttg ctacttttag ttgcttgtct
    44221 gttcatcgtg gcatatatcc cgcgcttgtt cccgatgctt tattttactc atcgaaaagt
    44281 gccatcctgg ttcagtgact ggatgaaata tgtaccggtg gcattatttg cggcgttagc
    44341 ttttaaagat gttttcatta ctcatgaaca tctggatatt gcttggaata tcaaaatagc
    44401 tgcaatgatt ctagtagcag gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt
    44461 gacaggactt gcgtctgtct ttttgctgtc tatgctttaa actgttttat tttattaaaa
    44521 acagagattg cgacataagt tgttcaaact gatctatccg aactgcattt gcaacagtca
    44581 attctggatg caagtttatt cgtagggata aaaaagatta gtgttggctt atgatgcaag
    44641 ctctttttta ttttaggaat tagagaggga tttgtccttc tatgattttt tgctataata
    44701 gggaagatgt ttattttatg aaagaatagt tgagggaatt taaatgaata aaaaattgat
    44761 gcaaggaaca ttttggctaa cctttgctaa tcttctatgt aaagttttag gggtagtcta
    44821 tttgattccc tggctaagta tgatggggaa caatcaagat ggtatgcttg ctacgacatt
    44881 gtataatgtt ggctatcttc cttatggatt gttcctgatg cttggtacag tcggatttcc
    44941 aaatgcgatt gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg
    45001 gctgattttt agaagtacga tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact
    45061 gatgtattta tttgctcctt tactagccgg aatcagtccg attgctaacg ttaataatgg
    45121 gatactagct atcagaagtt tatgcccatc tttgattgct atcccaattc ttagtgcaat
    45181 gagaggatat ttccaaggaa aaaatgatct gcggccttat ggaacttctt tgattatcga
    45241 acaggctgtg cgagtgatcg ttatcttggc tggtacttat tacttgcgcg tattgactga
    45301 cggaaccata ttagaagctg tattgatcag tacagtcgct tctttcttcg gtgggttagc
    45361 tgcgattatc catatgtata tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt
    45421 tttgatttcc agccgttatt tcgaaagaga aaaccgatct gcctctgtct ctatcattcg
    45481 ggaaacacta ccgtttattt ttgtcggctc agtcattact atcgttcaaa tgatcgacca
    45541 agtaacgatg aaaccactgc ttcacttttt ccgtccagag atcgcagatc agcaactgga
    45601 attcttattc agccgtgctt ctgtcaatcc aaataaattg acattgatcc tgatctccat
    45661 ggtaggaaca gtagctataa gcagcttgcc aatattaagc acgatgaaga aaaaagatcg
    45721 gatacaaatc gaaaaaacag taggagatag tttttcgatt gcgttattga ttcttcttcc
    45781 ttcattgact ggaatgtctt tattagctgg accactatac acattgtttt ttggctatga
    45841 tcctgaaagt gtcggatatt tccaaatggc tttgctagct tctttgtttt tctctttatt
    45901 cacgatccta tctacgatgc ttcaatcttt gaatcaccat cgttttgcga tccgattaac
    45961 tgtagaagca attatcctga aagtcatctt ccaagtaatc ggattaggat tagttggcgg
    46021 atacgggatg agcttatcaa atggcttagc attcggtatt gtctttgttc gcggctatca
    46081 ctttatgtgc aaagaatacc gaatagcacc tttagcaaag atcagcaatt ttttcttaaa
    46141 aactttccgc tccaccctga ttatgcttgc agtatgtttt gccgttttct ttttgctgaa
    46201 tcagcgattg tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag
    46261 tattggcggc ttagtatttt tattttctca attcggaaaa aatgggtttc gaatgttgaa
    46321 aaatgttaaa catcatagca gaagcaaaga ataggttaac taatcgtttt ttgctgtcac
    46381 ttgaaaaacg gcaactcgta tgattacgct tgtcgttttt ctattttcca attttagttt
    46441 catatctaat cttacttatg atatagttat aaggtctgga aaaattcttt gaaacgagga
    46501 aattaaacac atgaaacttg cagtagttac agatagttcg gcatatctgc cggaacgtat
    46561 caagaaccat cccgacttat tcgttattcc aatccctttg attatggatg ggaaaattta
    46621 taacgaagga atcgatatcg aagcggatga atattatgga cttttaaaaa acagtaaaga
    46681 ttttcctact acttcacaac ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa
    46741 agagaaaggg tatgacacga ttttaagtat ccatctttct tcagggattt ctggctttgt
    46801 caacacatta tttgcgatga aagatgacat cagcggagta acggtcattc cttatgactc
    46861 taagatcact agtatgccga tgggacacat ggtagaagct gccttagact taaatgatga
    46921 aggtcagtcc cttgatgaaa tacttgcaca tatcgatcga atccgagata atacctacgc
    46981 ttatctgatt gtagatgatt tgaacaattt agtacgagga ggacgtttga caaatggtgc
    47041 agcgctgatt ggcagcttat taaaaatcaa accgatcctg acattttctg aagggaaaat
    47101 tgttttattc gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg aagaaattat
    47161 tggtagaaga aataacgaaa tacaaccgcc agtcaaactt tacgtgatac atgcaaataa
    47221 tttagaagta gcgattgcgg agaaagagaa attgcagaaa aaatatccaa atgcagaaat
    47281 cgaaattggt catttcggac cagtcattgg tacacattta ggtgaaaaag ccatcggcat
    47341 tgccatttca gcgcaataat tccttttatt caaaaaacat accaaaaata ttccaacatc
    47401 atcttggact ttctacatgt tactaatgag cgtgtaaaag aacgagatgg tgtttttatt
    47461 tctatcaatg aagctctttt tctttgcaat cctcacttca atcctctata atgtcgcata
    47521 tagtttagta aatgagtaaa ctactaattt acttatttac taaaaataaa aggatgagga
    47581 gttaaaataa tggaaatacc aacaaattta aaacataaac cagtaatcgt tgtagaagac
    47641 tatgataaag ttgatggaag aaatgcatta aatacggatg caaaagggct ttcactgggc
    47701 cttgctcagt ggaatgatcg tggaaaagtc gatatctcag gcaaggtatg gcgacatact
    47761 ggagaaaaat ggtcacgaca gtcggaagaa ttaccaatca cacgtatttt agatttagcg
    47821 atcttagctg cacaaggaag tttatacttc caagatgctt atcgttatga aaaattttat
    47881 gatccagaaa atccagtcgt agacatcatt ggattacaag ggaaccgaat gacggtagaa
    47941 gtagatacca aaaatccaaa gatcgatgaa gacatcattt tgtattacga tacactacaa
    48001 aaagacggag aactgattgg ggaacgattc cgtattttaa aacgtctttt agatgagcta
    48061 ggatattaat agacagagat gatcaaaagg ggaagagaag catggagaat ttctcactaa
    48121 cagaaaaccg aaccttttca cggctagaaa agcaaatgat ctggaagaaa ccgaaaagcc
    48181 atcggacaag tgcagaagaa tggcggatcg cctccgaaat caaagggaat tggcaagatc
    48241 ctgaaatgaa aatcagtaac gtacttttgg aaggagatgc cgggtcgggg aagacacaat
    48301 tagccaaagc actatctgct gatctgcaac ttccctatac aaaagtgacc tgttttgcag
    48361 atatggacaa atcagatgtt ttcggagcct tattacctgt agtagattct aataaggaac
    48421 aagaccaaga attactagaa gctatttacc aaacagatac acttgaagct gtactgacac
    48481 tgatccagaa ccattacggc attgatcctt tttctgctaa agaaaaatta gcagagctag
    48541 ttcaaaggat cgaagctgat tatccagatg caatccatta cagctattac ccttctgaaa
    48601 tcgttcgagc gattgaaaaa ggatatttat tagagattca agagccgact gtcatccgag
    48661 atgcctctgt tcttgtggca cttaactcag cgctcgaacc aaatgggatg ttgaatttac
    48721 cgacaggaat cattcgtcgc catccagatt gcgtcattgt gatcactaca aatcgaaatt
    48781 accaaggaaa tcgcccatta aatgaatctt taagagatcg gatgcaacat gcggaaaaaa
    48841 tggatcttcc tgccttagaa gtcatggcag aacgtgcgat ggcgaaaact atgatccaaa
    48901 aacaagaact tctcttaaag atggcagaga ttatccgttt gttggatgaa actgcaaaag
    48961 ctaacgccat caaaggtgta gcaggaatgc gttcttattt ttattgggca aatacctata
    49021 agcaaggaca agatttactc cagtcgattt atccaaaagt tttgtataaa ttatccactg
    49081 attcagatga attggcaatt cttgaacaag ctttgtctga cagtggattg ctactggaac
    49141 ttcaggacat attgagaaaa gaaaaatggg gtcatcctca atctgagcat acaaaaggaa
    49201 gaaccattag cgccgaagaa gcaaatgaac gaaacatcac tgaaaatgct gaagaaattc
    49261 tgcgcttaga tgaaaagaaa tcgttgtctg ctgaagaacc aaatgaggga gaagagaaac
    49321 aagaagaaac aatagaaaaa aacgagattc ctcctcaaat gcaaaaagaa cgcagtagcg
    49381 atatcgaagg aaaaaatcaa aaagaacaac aaacaggaga tgattctcaa gaaatgtctg
    49441 cttctgacat ggaagcacat gataaggaaa tcaagaagca actgaataaa gaagcgcgac
    49501 aaatcatgaa ggaaacgatt catgaaaaag aaggtctcat cgttcaccga ccagctttcc
    49561 aaggaaaaac gccggaagca cgtgagctac acatgcaagt aatgccaata gtagaatcac
    49621 ttagccggca aattttagag ttactggata atgaacaaac agagacgtac caaaaaggaa
    49681 aatacgaagg tcagcggttc aatgcaagca aagtagcata tggtgatttg agaaattttg
    49741 acaaaaagaa cccgccacat gagcaacctt ctttagcggt agctgtacgg atcgatgaat
    49801 ctgggtcaat gatacgggat gatcggatcg aagcggcaaa aaaagcagcg attgccattg
    49861 ccgaattttc taagaaagtc gatatcccat tgttgattta cggagataca gcagaccgtt
    49921 cgtcaagaga aaagacttct ttgttttctt ataaagaatt tgaagacgga tttcaatggt
    49981 taaatgaaaa attagtcact atgaaaccaa ggcaaaacaa tcgtgatggt gctgcacttc
    50041 gtttagcagc agaaaaatta aacagacaat ccgcaacaac caaactgttg cttaatatta
    50101 gtgacggtca gccaaaagcg ttgccggatt atacaggcaa aaaagcaaaa gaagatatcc
    50161 agtctgtttt gaaagaatat gaacgtcaag gaatcctatt tgtctccgca gctattggcc
    50221 aagacaaaga agaaatcaag tcgatctacg gtgaaagtcg ttttgtagat attactgact
    50281 tgaatgaatt tcccaagcaa atgattcaac tgattgcccg ctatctatga taaaataaag
    50341 ctcaacgaac gcacttagca gattcgttga gcttttcgtt tctagttacc ggtaacaagg
    50401 atacttctaa aatagaatcg gttataatca agagggaccg ttaaaggagt gaaacgatat
    50461 gatacaagaa caaaaatggt ggaaagaagc agtaggctat caaatctatc ctgctagttt
    50521 caaagatagc aatggcgatg gtcaaggaga catcaatggg atccgtgaaa aacttaatta
    50581 tttgaaagat cttggtatcg gctttatctg gatcaatccg atctatcaat ctccatttgt
    50641 cgataatggt tatgacgtaa gtgactatca agatatagaa gagagattcg gaacgatgga
    50701 agattttgat ttattgctaa aagaagcaca tgaattaggg atcaaagtga ttatggatct
    50761 tgtcatcaac catagttcgg atcagcatca atggtttgaa gaatcgaaaa aatctaaaga
    50821 aagcccgtat cgcgattatt acatttgggt cgacggggta gatggcaaag aaccgaataa
    50881 ttggacatcg attttcggcg gttctgcttg ggagtattct catgaaactg gacagtatta
    50941 tctgcatgtt tttgcaaaag aacaaccaga cttaaattgg gaaagcgaga aattgaaaga
    51001 agaactattc aatatgatcc gatggtggtt ggataaaggg atagatggat ttcgtttaga
    51061 tgcgatttct catgtaaaaa aagatgaata ttcagtaaaa gccacagaga accctttctc
    51121 accctttcaa aatgtctcag gaatcgaaga acatttgacc gatttgaaac atgtttttga
    51181 agagtacgat atcatgactg tcggagaagc aagtggtgtc actgcagaag aaggaccaca
    51241 atgggtaggg aaagacggtt atttcgatat gatattcgaa tttgatcata tccatatctg
    51301 gcagcaagaa aaagaagggc aattagatgt ccttaaattg aaacttgcat taagcgcttg
    51361 gcaaacttca ttggatggta tcggatggaa cgctttatat atggaaaacc atgatgttcc
    51421 tcgtgccgtt tcagtgtttg gagatactcg tcccgatttt tgggctatgt ctgcaaaagc
    51481 aatcgctatg atgtatttct ttttgcaagg tacgccattt atttatcaag gacaggaaat
    51541 cggaatgacg aatatgccct ttgaatcgat cgatcaagtt gatgcagtag attcgaaaag
    51601 actttataaa aggctgcttg cggaaggtaa gacaagagaa gaagcattgg atatcattcg
    51661 tgaaacaacg agagacaaca gcaggacacc catgcaatgg acttcggaac aatatgctgg
    51721 tttttctaca catgaacctt ggctgatcac taatccaaat accaaaacga tcaatgtaga
    51781 acaacaagaa tatgaaccag agtcggtcct gcaattctat aagaacatga tccgcattag
    51841 acaaaccaat aaaggtctga tatacggttc ttataaagaa tatcttcatg aacatccaca
    51901 actttatgtc tatgaaagat atttagaaga tgaacaatat cttattatgg taaacttaac
    51961 agaatcactt gctgactatg aacttccaaa agaagcagat cagtcctgga cattactctt
    52021 gtcaaattcc tctagcggag aatttgaagc taaaggcata ctagcacctt atgaagctcg
    52081 gttatataaa acaaacaagt aaaacttcct cgttaaagtt cattagttgg actttgcgag
    52141 gaagtttttc agattgctct gtcaattact catttcaata attcgctcta ggaccaccga
    52201 tatatttagg acgagagcga agggctgtca cgtgagcttg tccaagggtt ttcaaacgag
    52261 gtctgacagg tacttggaca tgtttgatat gcataccgat tgatgtgtca ccgatatcga
    52321 taccggcttg tccaactacg tgttccactt cgacaggatc attgaactgg tcaaaagcag
    52381 caacagaaca agcacctcca gcatgtagtg caggaacaac agagacaatt tcgaaatttt
    52441 ttgcttctgc cactgttcgt tctactacta atgctcggtt caaatgttca caaccttgga
    52501 cagctagcgc aatttcttta ggatccaaca actcttttaa cgtacggatg atccattgac
    52561 caacttccgc actggaattt tttccaatat gtcctccaac gacttcgctt gtgctgcatc
    52621 ctaaaacaaa aagatcacct tttttcaagt gtgcctcagc caatatgtca ttaactattt
    52681 cagtcaattg gtcttttaat tctttttctg acaacttcat tttctaaaac cttctttttc
    52741 aattaatatt gcttccgtgc ttgaatacgt tgaagacttg cagttatagg aaaggcaagt
    52801 cccattccaa tcagattttg gaagatgttt cctggtatag aagccactcc agccggccaa
    52861 tcatataaaa tccaaccggc gataccatag ccgatgatca taactaagct tcctaataaa
    52921 agccctaatc cccacatacg agtagaagat ttgctgaaag aaccagcaac atatccttgt
    52981 aacccgtgga tcactaatga aaaaactgcc cactgcggat atcctgaaat caaatcgatc
    53041 aatagaccac tggaagcacc aacaactaat ccacccgttg gaccataaag aaaagcagcg
    53101 gtatagatcc cggcctcgca taatgtcaca aatccattcg tagccggtac agggatcaaa
    53161 attgttaatg aaagggcaac tgtcaacgca atcagcatag catagatcgt taattgctta
    53221 gttttcattt ctcattcctc ctttttgaat accgccagac cagacacttc cggtatgatc
    53281 gttcatcagc acgccattag aaatacaatg ggcaacaaat tttttacttt ctgctactgc
    53341 tttggtaaaa tctgccccta acacaagatt cgctgttatt gcagaagaaa aggcacaacc
    53401 ggccccgttt actgttgtag tgtcgatttt tttatgagaa aatacctcat aatgatcaat
    53461 tgaatagaga agatccacag cttcatctcc agccatcctt gtgccgcctt tgatcacaat
    53521 gttttttgtt cctgaagcag tgagttcttt agcgagatgg atcatttctg ttaacgaaga
    53581 gggaagagct tgaccagaaa gtaaagcagc ttctgccaaa ttaggcgtaa tcatcgtaac
    53641 aagtggaaaa agttcttcga tgagctgcga aatatacaat tgattgatca cagcttcagt
    53701 ttctttgaat gccaaaacag ggtctaaaat gattgggata ttttgttttg tttttatgaa
    53761 gtctcgaaca accatgattg cttcacttga attcaataac ccaattttga taccatctaa
    53821 tgaagtcatt ttatcaatcg aaactaactg tcgtttaagc aattccaaag atatagattc
    53881 cagcataaat cgctcattaa tatctagtgc ccctatagaa gtcaaggcgc ttagtccgaa
    53941 tagaccaaaa ttctcaaaag tttttaaatc tgtttggatt cctccaccag caaatggatc
    54001 agaaccgcca attgtcaaga ttcttttcat aacattttcc tccatgaata agagtataac
    54061 gaaattttca aaaaagaaaa acagccaatt ggcatatttt tatctcatcc aatttaaaaa
    54121 aacaaaaaga aaagaaagac caaaatggat ctttctttcc ttaaatagtt aggacttttg
    54181 tgattttaat tctttctcga tttgaagccg ttgcactttc attgtagctg ttcgcgggat
    54241 tgcatcaaaa tcacggatga tagggacgtt caagcgagga agttcatgga cttgctccca
    54301 ccatcgattc caatccattt ctttatcagg agctaaagca ataatcggtt gtggagaatg
    54361 atttttatcc cttacaatca ctacttctgc caaaaaatct aacgaatcta ataaaaagtc
    54421 ttcaatagct aatgtactat tgatcgtttc gatcaaatcc acttgccgat ctttcaaaaa
    54481 taggtggcca tgcttatcca taaaaccgta atctccgcta tcccaccaat cgccataaac
    54541 attttcctgg aaccgtgcat cttccttgta ataagtcaaa gcccgtcctt tagacagtaa
    54601 ttgtatatga ccatctgtgt tttcaggtag gacattccca actgaatctg taatccgagc
    54661 gcttgtcatg tcttccagtc caacgcccat atctcttgca tcacttgttt tcaaactctc
    54721 tagtgtatga gccttaagaa tcattggtcc gcattcactt tggccatata cttgaagaaa
    54781 taccgcatcc tgtgccatcg acgttcgcaa aaatgtcttc attgtctggt tgtttatagc
    54841 atcaaaagtg gagtgatagt atcggatacc cgaaaaagct tctggatgct ccttagcagt
    54901 aaaacgccac tgaacaaagt tattcggatg ggtctctagt gcaattggcc ggtgagcttc
    54961 caacatatga acaacttttg aactttgtgc gtttgctaaa ggcatcatag gaaatcccat
    55021 ggacatcaaa gaagagatac caatattgaa tctggaatgc actggagaga tgtgaaaagc
    55081 gattaatttc ttttccgaaa tttttgtgaa aactgttttt tgccatttcg tccgccatcc
    55141 catagaatga gcagaatgac aaataagttt agggatacca gttgtaccag aagtatgggt
    55201 catataagaa atttcatttt tgtctaaaaa tgattgagaa acagacaaag caggttgttc
    55261 cattaaataa agaatagg
//
LOCUS       DACEMS010000006        51320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEMS010000006 DACEMS010000000
VERSION     DACEMS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.006"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            290..1135
                     /locus_tag="IW735_000312"
     CDS             290..1135
                     /locus_tag="IW735_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1043339.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IW735_000313"
     CDS             1416..2636
                     /locus_tag="IW735_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1043340.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IW735_000314"
     CDS             2633..5353
                     /locus_tag="IW735_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1043341.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IW735_000315"
     CDS             5337..6281
                     /locus_tag="IW735_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1043342.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IW735_000316"
     CDS             complement(6350..7360)
                     /locus_tag="IW735_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1043343.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IW735_000317"
     CDS             complement(7540..7884)
                     /locus_tag="IW735_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1043344.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IW735_000318"
     CDS             complement(7877..8311)
                     /locus_tag="IW735_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1043345.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IW735_000319"
     CDS             8449..9186
                     /locus_tag="IW735_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043346.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IW735_000320"
     CDS             9179..10390
                     /locus_tag="IW735_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1043347.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IW735_000321"
     CDS             10597..11376
                     /locus_tag="IW735_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1043348.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IW735_000322"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IW735_000322"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1043349.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IW735_000323"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IW735_000323"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1043350.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IW735_000324"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IW735_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1043351.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IW735_000325"
     CDS             14978..16027
                     /locus_tag="IW735_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1043352.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IW735_000326"
     CDS             16024..16302
                     /locus_tag="IW735_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1043353.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IW735_000327"
     CDS             16277..16747
                     /locus_tag="IW735_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1043354.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IW735_000328"
     CDS             16764..17066
                     /locus_tag="IW735_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043355.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IW735_000329"
     CDS             17083..17454
                     /locus_tag="IW735_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1043356.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IW735_000330"
     CDS             17426..17785
                     /locus_tag="IW735_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043357.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IW735_000331"
     CDS             17957..18964
                     /locus_tag="IW735_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1043358.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IW735_000332"
     CDS             18989..20173
                     /locus_tag="IW735_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1043359.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IW735_000333"
     CDS             complement(20610..22193)
                     /locus_tag="IW735_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1043360.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IW735_000334"
     CDS             complement(22771..22914)
                     /locus_tag="IW735_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1043361.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23136..23615)
                     /locus_tag="IW735_000335"
     CDS             complement(23136..23615)
                     /locus_tag="IW735_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1043362.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23688..23972)
                     /locus_tag="IW735_000336"
     CDS             complement(23688..23972)
                     /locus_tag="IW735_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043363.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24050..24244)
                     /gene="ssrS"
                     /locus_tag="IW735_000337"
     ncRNA           complement(24050..24244)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW735_000337"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24316..25602)
                     /locus_tag="IW735_000338"
     CDS             complement(24316..25602)
                     /locus_tag="IW735_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1043364.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25740..26411
                     /locus_tag="IW735_000339"
     CDS             25740..26411
                     /locus_tag="IW735_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1043365.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26377..26865
                     /locus_tag="IW735_000340"
     CDS             26377..26865
                     /locus_tag="IW735_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1043366.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26878..27825
                     /gene="prmA"
                     /locus_tag="IW735_000341"
     CDS             26878..27825
                     /gene="prmA"
                     /locus_tag="IW735_000341"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1043367.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27826..28590
                     /locus_tag="IW735_000342"
     CDS             27826..28590
                     /locus_tag="IW735_000342"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1043368.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITKITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28823..31036
                     /locus_tag="IW735_000343"
     CDS             28823..31036
                     /locus_tag="IW735_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1043369.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31047..31493
                     /locus_tag="IW735_000344"
     CDS             31047..31493
                     /locus_tag="IW735_000344"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1043370.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31664..32923
                     /locus_tag="IW735_000345"
     CDS             31664..32923
                     /locus_tag="IW735_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1043371.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33012..33959)
                     /locus_tag="IW735_000346"
     CDS             complement(33012..33959)
                     /locus_tag="IW735_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1043372.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34353..35645
                     /locus_tag="IW735_000347"
     CDS             34353..35645
                     /locus_tag="IW735_000347"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1043373.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35660..37429
                     /gene="aspS"
                     /locus_tag="IW735_000348"
     CDS             35660..37429
                     /gene="aspS"
                     /locus_tag="IW735_000348"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1043374.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37538..38413
                     /locus_tag="IW735_000349"
     CDS             37538..38413
                     /locus_tag="IW735_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1043375.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38679..40562
                     /locus_tag="IW735_000350"
     CDS             38679..40562
                     /locus_tag="IW735_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1043376.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40637..40921)
                     /locus_tag="IW735_000351"
     CDS             complement(40637..40921)
                     /locus_tag="IW735_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1043377.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40911..41645)
                     /locus_tag="IW735_000352"
     CDS             complement(40911..41645)
                     /locus_tag="IW735_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1043378.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41774..42397
                     /locus_tag="IW735_000353"
     CDS             41774..42397
                     /locus_tag="IW735_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1043379.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42589..43710
                     /locus_tag="IW735_000354"
     CDS             42589..43710
                     /locus_tag="IW735_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1043380.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43717..46845
                     /locus_tag="IW735_000355"
     CDS             43717..46845
                     /locus_tag="IW735_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1043381.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46864..50058
                     /locus_tag="IW735_000356"
     CDS             46864..50058
                     /locus_tag="IW735_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1043382.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50204..50911)
                     /locus_tag="IW735_000357"
     CDS             complement(50204..50911)
                     /locus_tag="IW735_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1043383.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16806 a         8957 c        10642 g        14915 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    23041 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    23101 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    23161 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23221 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23281 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23341 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23401 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23461 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23521 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23581 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23641 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23701 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23761 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23821 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23881 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23941 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    24001 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    24061 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    24121 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24181 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24241 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24301 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24361 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24421 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24481 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24541 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24601 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24661 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24721 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24781 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24841 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24901 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24961 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    25021 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    25081 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    25141 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25201 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25261 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25321 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25381 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25441 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25501 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25561 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25621 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25681 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25741 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25801 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25861 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25921 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25981 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    26041 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    26101 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    26161 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26221 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26281 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26341 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26401 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26461 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26521 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26581 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26641 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26701 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26761 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26821 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26881 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26941 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    27001 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    27061 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    27121 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27181 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27241 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27301 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27361 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27421 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27481 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27541 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27601 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27661 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27721 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27781 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27841 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27901 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27961 atagccattg ttgcagaaat cacgaagatt actgaagagg ctgtttatct gaaagaaatt
    28021 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    28081 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    28141 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28201 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28261 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28321 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28381 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28441 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28501 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28561 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28621 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28681 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28741 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28801 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28861 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28921 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28981 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    29041 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    29101 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    29161 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29221 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29281 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29341 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29401 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29461 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29521 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29581 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29641 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29701 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29761 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29821 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29881 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29941 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    30001 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    30061 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    30121 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30181 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30241 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30301 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30361 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30421 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30481 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30541 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30601 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30661 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30721 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30781 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30841 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30901 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30961 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    31021 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    31081 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    31141 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31201 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31261 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31321 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31381 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31441 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31501 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31561 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31621 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31681 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31741 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31801 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31861 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31921 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31981 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    32041 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    32101 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    32161 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32221 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32281 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32341 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32401 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32461 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32521 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32581 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32641 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32701 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32761 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32821 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32881 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32941 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    33001 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    33061 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    33121 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33181 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33241 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33301 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33361 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33421 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33481 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33541 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33601 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33661 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33721 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33781 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33841 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33901 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33961 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    34021 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    34081 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    34141 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34201 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34261 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34321 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34381 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34441 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34501 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34561 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34621 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34681 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34741 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34801 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34861 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34921 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34981 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    35041 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    35101 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    35161 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35221 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35281 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35341 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35401 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35461 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35521 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35581 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35641 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35701 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35761 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35821 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35881 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35941 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    36001 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    36061 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    36121 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36181 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36241 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36301 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36361 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36421 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36481 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36541 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36601 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36661 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36721 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36781 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36841 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36901 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36961 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    37021 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    37081 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    37141 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37201 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37261 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37321 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37381 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37441 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37501 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37561 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37621 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37681 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37741 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37801 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37861 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37921 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37981 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    38041 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    38101 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    38161 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38221 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38281 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38341 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38401 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38461 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38521 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38581 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38641 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38701 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38761 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38821 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38881 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38941 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    39001 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    39061 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    39121 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39181 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39241 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39301 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39361 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39421 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39481 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39541 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39601 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39661 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39721 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39781 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39841 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39901 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39961 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    40021 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    40081 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    40141 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40201 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40261 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40321 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40381 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40441 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40501 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40561 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40621 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40681 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40741 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40801 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40861 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40921 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40981 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    41041 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    41101 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    41161 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41221 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41281 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41341 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41401 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41461 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41521 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41581 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41641 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41701 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41761 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41821 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41881 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41941 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    42001 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    42061 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    42121 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42181 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42241 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42301 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42361 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42421 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42481 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42541 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42601 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42661 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42721 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42781 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42841 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42901 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42961 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    43021 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    43081 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    43141 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43201 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43261 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43321 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43381 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43441 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43501 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43561 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43621 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43681 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43741 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43801 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43861 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43921 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43981 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    44041 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    44101 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    44161 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44221 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44281 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44341 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44401 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44461 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44521 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44581 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44641 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44701 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44761 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44821 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44881 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44941 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    45001 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    45061 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    45121 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45181 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45241 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45301 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45361 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45421 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45481 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45541 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45601 agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45661 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45721 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45781 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45841 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45901 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45961 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    46021 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    46081 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    46141 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46201 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46261 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46321 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46381 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46441 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46501 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    46561 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46621 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46681 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46741 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46801 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46861 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46921 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46981 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    47041 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    47101 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    47161 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47221 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47281 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47341 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47401 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47461 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47521 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47581 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47641 caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa
    47701 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47761 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47821 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47881 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47941 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    48001 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    48061 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    48121 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48181 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48241 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48301 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48361 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48421 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48481 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48541 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48601 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48661 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48721 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48781 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48841 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48901 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48961 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    49021 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    49081 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    49141 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49201 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49261 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49321 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49381 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49441 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49501 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49561 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49621 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49681 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49741 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49801 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49861 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49921 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49981 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    50041 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    50101 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    50161 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50221 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50281 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50341 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50401 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50461 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50521 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50581 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50641 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50701 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50761 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50821 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50881 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50941 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    51001 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    51061 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    51121 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt
    51181 taatacagtc tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgttta
    51241 tagtaaattt tatttagtaa tcattgcttt aaaatactac gcttatataa cagagaaaat
    51301 aagcgttata ataaagaaca
//
LOCUS       DACEMS010000007        47163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEMS010000007 DACEMS010000000
VERSION     DACEMS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.007"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(187..1578)
                     /locus_tag="IW735_000358"
     CDS             complement(187..1578)
                     /locus_tag="IW735_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1043384.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1791..2177)
                     /locus_tag="IW735_000359"
     CDS             complement(1791..2177)
                     /locus_tag="IW735_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1043385.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2233..2856)
                     /locus_tag="IW735_000360"
     CDS             complement(2233..2856)
                     /locus_tag="IW735_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1043386.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2920..3588)
                     /locus_tag="IW735_000361"
     CDS             complement(2920..3588)
                     /locus_tag="IW735_000361"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1043387.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3600..4040)
                     /locus_tag="IW735_000362"
     CDS             complement(3600..4040)
                     /locus_tag="IW735_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043388.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4099..4770)
                     /locus_tag="IW735_000363"
     CDS             complement(4099..4770)
                     /locus_tag="IW735_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1043389.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4955..5353)
                     /locus_tag="IW735_000364"
     CDS             complement(4955..5353)
                     /locus_tag="IW735_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1043390.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5350..6123)
                     /locus_tag="IW735_000365"
     CDS             complement(5350..6123)
                     /locus_tag="IW735_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1043391.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6120..6803)
                     /locus_tag="IW735_000366"
     CDS             complement(6120..6803)
                     /locus_tag="IW735_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1043392.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6970..7857)
                     /locus_tag="IW735_000367"
     CDS             complement(6970..7857)
                     /locus_tag="IW735_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1043393.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7854..8723)
                     /locus_tag="IW735_000368"
     CDS             complement(7854..8723)
                     /locus_tag="IW735_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1043394.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8860..10146)
                     /locus_tag="IW735_000369"
     CDS             complement(8860..10146)
                     /locus_tag="IW735_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1043395.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10419..10997
                     /locus_tag="IW735_000370"
     CDS             10419..10997
                     /locus_tag="IW735_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1043396.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10997..11926
                     /locus_tag="IW735_000371"
     CDS             10997..11926
                     /locus_tag="IW735_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1043397.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11923..13659
                     /locus_tag="IW735_000372"
     CDS             11923..13659
                     /locus_tag="IW735_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1043398.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13722..14558)
                     /gene="pheA"
                     /locus_tag="IW735_000373"
     CDS             complement(13722..14558)
                     /gene="pheA"
                     /locus_tag="IW735_000373"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1043399.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14555..15064)
                     /locus_tag="IW735_000374"
     CDS             complement(14555..15064)
                     /locus_tag="IW735_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1043400.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15057..16352)
                     /gene="aroA"
                     /locus_tag="IW735_000375"
     CDS             complement(15057..16352)
                     /gene="aroA"
                     /locus_tag="IW735_000375"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1043401.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16365..17456)
                     /locus_tag="IW735_000376"
     CDS             complement(16365..17456)
                     /locus_tag="IW735_000376"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1043402.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17473..18639)
                     /gene="aroC"
                     /locus_tag="IW735_000377"
     CDS             complement(17473..18639)
                     /gene="aroC"
                     /locus_tag="IW735_000377"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1043403.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18641..19705)
                     /gene="aroB"
                     /locus_tag="IW735_000378"
     CDS             complement(18641..19705)
                     /gene="aroB"
                     /locus_tag="IW735_000378"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1043404.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19738..20760)
                     /gene="aroF"
                     /locus_tag="IW735_000379"
     CDS             complement(19738..20760)
                     /gene="aroF"
                     /locus_tag="IW735_000379"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1043405.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21419..22393)
                     /locus_tag="IW735_000380"
     CDS             complement(21419..22393)
                     /locus_tag="IW735_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1043406.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22374..24257)
                     /locus_tag="IW735_000381"
     CDS             complement(22374..24257)
                     /locus_tag="IW735_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1043407.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24275..26500)
                     /locus_tag="IW735_000382"
     CDS             complement(24275..26500)
                     /locus_tag="IW735_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1043408.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26615..27649
                     /locus_tag="IW735_000383"
     CDS             26615..27649
                     /locus_tag="IW735_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1043409.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27716..28729)
                     /locus_tag="IW735_000384"
     CDS             complement(27716..28729)
                     /locus_tag="IW735_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1043410.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28807..28995)
                     /locus_tag="IW735_000385"
     CDS             complement(28807..28995)
                     /locus_tag="IW735_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1043411.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29435..29563)
                     /locus_tag="IW735_000386"
     CDS             complement(29435..29563)
                     /locus_tag="IW735_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1043412.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29566..30939)
                     /gene="rlmD"
                     /locus_tag="IW735_000387"
     CDS             complement(29566..30939)
                     /gene="rlmD"
                     /locus_tag="IW735_000387"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1043413.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31050..32105)
                     /locus_tag="IW735_000388"
     CDS             complement(31050..32105)
                     /locus_tag="IW735_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1043414.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32115..33545)
                     /gene="gatB"
                     /locus_tag="IW735_000389"
     CDS             complement(32115..33545)
                     /gene="gatB"
                     /locus_tag="IW735_000389"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1043415.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33542..35014)
                     /gene="gatA"
                     /locus_tag="IW735_000390"
     CDS             complement(33542..35014)
                     /gene="gatA"
                     /locus_tag="IW735_000390"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1043416.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35014..35319)
                     /gene="gatC"
                     /locus_tag="IW735_000391"
     CDS             complement(35014..35319)
                     /gene="gatC"
                     /locus_tag="IW735_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1043417.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35425..37461)
                     /gene="ligA"
                     /locus_tag="IW735_000392"
     CDS             complement(35425..37461)
                     /gene="ligA"
                     /locus_tag="IW735_000392"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1043418.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37478..39712)
                     /gene="pcrA"
                     /locus_tag="IW735_000393"
     CDS             complement(37478..39712)
                     /gene="pcrA"
                     /locus_tag="IW735_000393"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1043419.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39867..41426
                     /locus_tag="IW735_000394"
     CDS             39867..41426
                     /locus_tag="IW735_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1043420.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41537..43468)
                     /locus_tag="IW735_000395"
     CDS             complement(41537..43468)
                     /locus_tag="IW735_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1043421.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43486..44400)
                     /gene="pfkB"
                     /locus_tag="IW735_000396"
     CDS             complement(43486..44400)
                     /gene="pfkB"
                     /locus_tag="IW735_000396"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1043422.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44397..45149)
                     /locus_tag="IW735_000397"
     CDS             complement(44397..45149)
                     /locus_tag="IW735_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1043423.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45309..45518)
                     /locus_tag="IW735_000398"
     CDS             complement(45309..45518)
                     /locus_tag="IW735_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043424.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45529..46857)
                     /locus_tag="IW735_000399"
     CDS             complement(45529..46857)
                     /locus_tag="IW735_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043425.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        13153 a        10190 c         8408 g        15412 t
ORIGIN      
        1 actatttttg tttggctgtt cttattactt gatgcagggc agctttttca caacctctta
       61 ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa aaatttgaga
      121 aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat cacaacctct
      181 ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat cgcgatctaa
      241 atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga cgtttactgc
      301 tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga ttgctactgg
      361 caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac cgactccagc
      421 tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt ccaaactaaa
      481 gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat aaacgccagc
      541 acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg ttgcatcatc
      601 tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag cacttgaacg
      661 tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga ttggattgac
      721 ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg ctacgtaaga
      781 agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg ccaaaacgtt
      841 tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca cgatccgcat
      901 aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt ttggcgcttt
      961 tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga ctccaatcgt
     1021 acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa aggataaaat
     1081 ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt cagagattcg
     1141 tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca gcattgttga
     1201 accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca atacagtcgc
     1261 actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa tgatcgaaat
     1321 aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac ctacaatccc
     1381 gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca attgaagcag
     1441 accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa attttgtatg
     1501 gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa ccactaaaac
     1561 tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc catcgaagga
     1621 agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt tttcagtgac
     1681 ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa ttagtaataa
     1741 aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt ctattcttgt
     1801 ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc tgtcacctca
     1861 taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac ttcttcttgt
     1921 gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg actcacactt
     1981 ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc tacgatgcta
     2041 tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac tttcaaatct
     2101 gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac aacttctttt
     2161 tgtcttctga tttccatata attttatcac tcctttatga aaattataac atatctcctt
     2221 tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat tcagatttcc
     2281 taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa gacaagtagc
     2341 gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca tcatcgtgtc
     2401 tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat caggtttacg
     2461 aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc gatgttcttt
     2521 taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac gaccgccgtt
     2581 gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa cttccgtact
     2641 ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt ttgattcatt
     2701 gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt tgtccatact
     2761 gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat acgtatcaaa
     2821 caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc attcctttgc
     2881 ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt ttttgtccag
     2941 aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga tatcgatcgt
     3001 atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga tgctattacc
     3061 atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat gatcttgcca
     3121 taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga tcaaatgcgg
     3181 atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg gaacttccgt
     3241 atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca tcttatcttt
     3301 gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt ttattatagg
     3361 aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg cacgtttcaa
     3421 aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt gaccggcaac
     3481 tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg tccagtaatt
     3541 ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc ttttctccct
     3601 taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat tcgtcctttt
     3661 cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt ataacttttt
     3721 cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt gactctatca
     3781 agatatttca aataacgttg attctcattt agtctggggt agattttttt tgataggaaa
     3841 cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct atagggcaac
     3901 caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt caactgatca
     3961 aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg tattagcctt
     4021 tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta tgacaaaaaa
     4081 atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa ggacattccg
     4141 atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga ctactcgaaa
     4201 tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac gtataaaacg
     4261 atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac ctggatattt
     4321 tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt ttaaaaagct
     4381 ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat ctatttgata
     4441 acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt ttgtatcgat
     4501 cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa gagaaagctc
     4561 gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa cagccgacag
     4621 cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact ctggtgaaaa
     4681 atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc gctcaatcag
     4741 agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat catttttaaa
     4801 atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa aatttactat
     4861 ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct catccgcctt
     4921 cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt tctctatcgt
     4981 tattttcatc taaaagttct tgcattccga taatttctcg atattgatga atcttagtga
     5041 taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga atatcgatgt
     5101 tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt actaatgtca
     5161 acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa tcatgtacac
     5221 cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt gaaaagtcga
     5281 taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga ttcaatgcta
     5341 caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga ttctgtcttt
     5401 ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg gtgtcaatgc
     5461 aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa tttgtgcagc
     5521 gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg ctactccacc
     5581 gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa taaatgctag
     5641 cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac gccacataga
     5701 caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt tcgatcggat
     5761 tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg gctcagcaaa
     5821 ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt aaggcaatcc
     5881 cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga ctccgtgcag
     5941 gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa agaaacgctt
     6001 tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga taggtgccac
     6061 tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct gccagaaact
     6121 cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga agcaataccg
     6181 accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt catccctccg
     6241 gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc cgaatcaaaa
     6301 gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc tatgtagcct
     6361 atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc aaaggctaga
     6421 aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat ctctccgtca
     6481 gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat cgtgatcacg
     6541 atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt gatcatatat
     6601 ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa aaagagcgtc
     6661 attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc tccttctaac
     6721 ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat agcagcaatc
     6781 acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt taccttaaaa
     6841 tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc aacataaaat
     6901 aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga acgcgcttat
     6961 tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt gcaaaacaaa
     7021 aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca ttccatagtc
     7081 tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt tatcaggaga
     7141 ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg tgatgattgc
     7201 agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa tacttagttc
     7261 ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt taacattttg
     7321 tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa ccaagaaact
     7381 gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag ggatcatcaa
     7441 tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt cttttcgttt
     7501 actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg caaacacagt
     7561 ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca gctcattgga
     7621 aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc ccgatgtgat
     7681 atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa tggaaataaa
     7741 tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac gaagttctaa
     7801 tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat ttttcatcga
     7861 ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga aacaataaca
     7921 aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa attatagatg
     7981 tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt ttgtacttca
     8041 tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat gattggtttt
     8101 aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc aatagatgct
     8161 gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat catattgtat
     8221 ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat tggatgagtc
     8281 aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt tacaaggcca
     8341 gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt gatacatgga
     8401 agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt taaaaggttg
     8461 gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata caggaatgta
     8521 ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt acctaacccg
     8581 ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc caaaaccatc
     8641 ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc atgcccgttt
     8701 ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat tatctacatg
     8761 aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct tttcgtttgt
     8821 ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca attgtgcttc
     8881 tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga tgattcgatg
     8941 aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat tcacttctgg
     9001 tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt aaaattcaga
     9061 agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta gtccgatttc
     9121 tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcttcta ggaaatcttt
     9181 cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc caaggttaga
     9241 agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct tccattttcc
     9301 agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg ggctagaagc
     9361 tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt gttcgactag
     9421 atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg cattgtcttg
     9481 gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt gcataatcat
     9541 ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg tcttttcttt
     9601 gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat cctcttctgt
     9661 ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc ccgaatcgaa
     9721 aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg gtgcaaaatg
     9781 atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag tcaaatagcc
     9841 ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat tcccgctcgc
     9901 taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa cttctatctc
     9961 tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat tgaatgtctc
    10021 atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag cacttccgca
    10081 tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta actttctgat
    10141 gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta cattcatcat
    10201 accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc tcctaaaata
    10261 ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag accaaagatc
    10321 gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag aaataagtat
    10381 ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc gtaattctta
    10441 tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg tctgagcacg
    10501 ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt tctttggtac
    10561 atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga atcgaattat
    10621 cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct agctatgatg
    10681 aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt ttaaagcctc
    10741 gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa atcaaaaaaa
    10801 agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat ctttcacagc
    10861 agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac accttgtttt
    10921 tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac aaaaacagtc
    10981 gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg tttctacctt
    11041 ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta ggaagtttgt
    11101 tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg attcttttgc
    11161 aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag gacaacgttt
    11221 ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat taaccaacca
    11281 cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg ctttagctgt
    11341 caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc cagcttattt
    11401 gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg acgagcatgt
    11461 tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag ccgatgctga
    11521 gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca cccacttttc
    11581 tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt tcgtccagga
    11641 aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt tttcatatag
    11701 ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat atctaaaaaa
    11761 agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt tgacggaaat
    11821 ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta aagaagagac
    11881 aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa aaaaatcaaa
    11941 gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt tctgcaagcc
    12001 atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca gctgttcaat
    12061 caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt gaatgataca
    12121 atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta tttttccaat
    12181 gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa aatgaatgac
    12241 tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg agaaaatgga
    12301 agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt ttttcaatct
    12361 gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt tgcacaccac
    12421 ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa actgaaaaat
    12481 acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca actagcgaat
    12541 ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa cgaagaacag
    12601 acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat tttcaaaaaa
    12661 ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct tcctctgtac
    12721 cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa ccaaatgggc
    12781 gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg ctcattggta
    12841 tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc gctaaaacgg
    12901 ctagaggaag atggcttaaa agctactgtt cctgtacaag gaagccatga agtccaattg
    12961 ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga acagacgatc
    13021 caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca gatacagccc
    13081 cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa aaaaacagat
    13141 gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat tgggaaaaaa
    13201 gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat cgatcttttg
    13261 agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga actgcagtca
    13321 ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct tcatgctttt
    13381 cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca gcttctttta
    13441 ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca aaaaaatgat
    13501 catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat gtatggcgat
    13561 gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac gatttgtcta
    13621 ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa gcgctgattt
    13681 ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg cgtctcttct
    13741 actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat cttgatttct
    13801 tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat cacgaaaaaa
    13861 tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt gatttcccgc
    13921 cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg tagtgtcaag
    13981 atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc accgacgatc
    14041 caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat ctccaatcca
    14101 acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg atgttctgct
    14161 acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt aggataatgc
    14221 gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat ttttgtaatc
    14281 tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc acttttaact
    14341 tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc ttccaatgaa
    14401 ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg caaacaaaca
    14461 gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc cgcctgataa
    14521 gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc ctttctataa
    14581 tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt gtcgcacttt
    14641 cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt gtttttccaa
    14701 gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga cagtccgctt
    14761 ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt tctgcacttt
    14821 ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt cttaaaatat
    14881 ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg gcaatcgaca
    14941 attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt aaacattgag
    15001 ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc aaaatgctag
    15061 ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc cggataagag
    15121 attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa agcagctact
    15181 tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc atgcagaggt
    15241 gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc cattttctgt
    15301 aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc ttctgcgtct
    15361 ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg caattcatca
    15421 atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact cgtcttgaca
    15481 cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc tgcgcccatc
    15541 tcctccaaca catctaagat accagttctt gttggattga ccccgacatt ttttaacaac
    15601 agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga tgaaatatca
    15661 cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat gatcgtttta
    15721 tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg gttacgggaa
    15781 cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa cagcaaagca
    15841 gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa agagcgggtt
    15901 cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc agcgcccatc
    15961 tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc atccccgaac
    16021 agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat ggtcgtaccg
    16081 gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc gcgaccatga
    16141 atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa ggcatgtaaa
    16201 gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc ttcagcaatt
    16261 gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct aacagtccct
    16321 cttaatccat gtatttgttg taataattgc atttatccag cctcctattt gatccagcaa
    16381 tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg ttggttttta
    16441 aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt caaattaatc
    16501 aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc aggttcatct
    16561 ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg gacaggcaac
    16621 tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg gttctcagtc
    16681 atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg ttccaataat
    16741 atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga agcgatccta
    16801 gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc ttgattgaat
    16861 tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact tagcattcct
    16921 gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt tccgcaaaat
    16981 agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa gatgtaatag
    17041 gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga accagccatt
    17101 ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac ttgctgcttc
    17161 gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc aaggacatca
    17221 agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc ttgttctgca
    17281 cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc ttgctcacgg
    17341 gtttttgact gactatccca accaactact atcagttctg gattggtctg acgaatacaa
    17401 agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg tgccatctta
    17461 tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg cagctttcaa
    17521 ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca cagtggctac
    17581 aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac gttctacact
    17641 tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt acaatgtagg
    17701 aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc cgccttcgaa
    17761 gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata ggatctcatc
    17821 catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt ctacgccttt
    17881 aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg tgtcccattg
    17941 tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa ttcctccgat
    18001 cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct caactgctgg
    18061 atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa tcgagatatt
    18121 tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta cgatgatgtc
    18181 aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg tcgtctctct
    18241 tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca taccgcctac
    18301 caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt ctttttcagg
    18361 cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt ctaccactaa
    18421 agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc gaacacgatc
    18481 tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag cgagttcgtg
    18541 attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa tggctgttaa
    18601 ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct cctaagtaat
    18661 ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt tctagcaaga
    18721 tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg aataattcat
    18781 ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc ctttctaaag
    18841 cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg atggctacca
    18901 ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta ttttcaattg
    18961 catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca aagacatctt
    19021 cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca gcatgttcca
    19081 gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca tctgctatag
    19141 ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc aaagttttta
    19201 gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca aggtttttcg
    19261 ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt gcaagtagcg
    19321 tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg aatgctgcta
    19381 gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta aaatcattat
    19441 ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc tcacccgccg
    19501 gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg attttttctg
    19561 catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt ctccaaatcg
    19621 tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata tcgtattttt
    19681 ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa gctggaatta
    19741 atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt tcatcatgcg
    19801 tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg caggatcagg
    19861 atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag ccattggtgg
    19921 tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga aatgagttaa
    19981 ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg tttcgtaagt
    20041 ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa tgtattctgc
    20101 agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa caggtttacc
    20161 tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc caatctgtag
    20221 gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt cagtaatcac
    20281 tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt cttctaaacc
    20341 ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa tttttgcgcc
    20401 cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga tagaacaagg
    20461 accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat ccacgactgt
    20521 attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac gcacagcatt
    20581 ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat caccttttag
    20641 tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt ctttttcgat
    20701 atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa ttactatcat
    20761 tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc tactttttca
    20821 aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca ttttcgctcc
    20881 ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta ggaaattgcc
    20941 tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa aatagccact
    21001 atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc catcatagat
    21061 acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta aaatagctaa
    21121 agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc tccaaactga
    21181 tgtaagaatt gtacctagta tagacataaa aaaatcgact gtcaatcatt tttatgaaac
    21241 tttttttaat tttctgatag ttaactttgt ttttcagaaa aataacaatt ggttttattg
    21301 aagaaaatag tcatgaacaa aagtatctga gaactttttt actctcttgt tcatgacata
    21361 tagaataaag cagctttgtc ttgtaaactc cgtttttcaa ataataatct aaagattgct
    21421 actgaataat aaagccgatt tcatggtcct cttcgctgct gattcggtaa ggtgactcaa
    21481 catccgctcc ccagctattg atacccccga ctcctcttac ttttgcaaaa atcgtaagaa
    21541 ccgttcgtct ttctggcggt agttcttcta tatgtgttgc ctgttccagt tcttctgctt
    21601 tgaatggtaa agcggaaaaa gcaaatggcg tatcctgctg ttcaaatcgc aacgaaaaat
    21661 cttcttgatc tttatccgta ttatccaacg tttgatttct tgttagttct acccatttcg
    21721 tttccatatg catgccgcat tcttggggaa caaggtatgg ggaaacgggg agtcctgcca
    21781 ctttataaat accttcgaca ccaccagcca tccgatccgg atatgtttcc cctgataggc
    21841 cttcatactc aaaaaatttt gctttcgttg gcataatgaa ccttaaaccc aatgcgggta
    21901 attccggtaa tcctttttgt ccataataat gcatattgac aagcatttta ccctctccag
    21961 cgatttggta ggaaattgtc acttgggttt ttggtactgt gttgatttcc agtgtaaaaa
    22021 tgatttcaac cgtttcagcg tattcattac ttgaatattg attatttact ggagcaatcg
    22081 gcaaatcgat ctttttattg tccaccagta cttctatttc tttgactttt ggatacaatc
    22141 cggctcctaa ccacataccg gatttaaatc caaatccgct acctctgtca ttatcggtcg
    22201 ttgctcgcca aaaaactgga agtgtttccc ggtatagcca ttcttttccg tttttattga
    22261 gcgattccag tccgcctctt gtataagaaa aaatataatg cgtattcccc gatttgacac
    22321 ctaatgtaca gtcgccgaag acgattgcaa ctttttccgt atccatatga cttttattta
    22381 ctgtattttt cataataata tcttacctcc tgcatggcgg gtttttcttg tccatctgca
    22441 aaaacgatcc cattccctga gaattcatag tcacttggtt tatcgtcaaa gtcaccgcca
    22501 tagcgcatca cttctctgcc tgtgattgga tcagtcactt ggatcgcctg atctttaaaa
    22561 tcccatatat agcctccttg gtacatttca taacgatcaa gcaacgtcat gtaagaatcc
    22621 attccaccta acgaattccc catgtcatgc atatattcac ataagatgaa cggtttttcc
    22681 gctttatttt caaggtagtc aatgatctct tctggtggcg catacatgca gctttttaca
    22741 tctgaaatcg aatcttcgaa tgctggattt ggaaaattcc cttcataatg gacaagtcga
    22801 tcggggtcta cttttttgaa gtaatcgttc atttgtcgaa tgttttcttc cgcataagat
    22861 tcatttccaa gagaccaaaa aagaatactt ggatggtttt ttaatgtttc gaaattcgtt
    22921 tttgctcgat caagaacagc cgcctgccac tgaggtactg cacctggtac gttgtaagaa
    22981 ggctcaaatg cgcccatttt ttgccatgag ccatgggatt cgaggttcgt ttccgccatc
    23041 acatggattc ctgcttggtc gcataaataa taccaagtga tctggttggg gtaatgagat
    23101 gtccgtacgc cgttgatctt tgcatttttt atgatagcca aatctttttc catatcttct
    23161 atagtgatgg cgcgtccttt tttcggatgc cactcatgtc gatttacccc gttgataatc
    23221 atccgttttc cattgaagta gatgatttta tctttgattt cgattttacg aaaaccgaag
    23281 gaatagtcaa tatactccgt tactcttttt tcttcgtcta tcgtctccaa ttcaagctta
    23341 tataaattgg gctgaaaatg cccccacagt gccacatttt caaatccatc gatgcgaatc
    23401 gtcatttgct cttttaacgg atattgctgt tgatagattt ccttcccatc aggatctagt
    23461 atcgctacat tcagctgact gtttttggct acttgctcaa aagacaaatc tacttcaagt
    23521 ttgccatttt gacatttgtc atctaagcga ggtttgatcc aaagatcttc taaatggttt
    23581 tgtcctcgag caactaagaa gacatctctg aagatcccaa aaaatcggaa aaagtcctga
    23641 tcttccaaga atgcagctgt acttcttttg taaacctcca cagctagaat attttcttct
    23701 tccaaataag gtgttaaatc aaattcagac ggagtgaacg aatcttcggc atacccgata
    23761 aaatgaccat tcaaccaaat aaacattgct tgttctacac cagcaaaatg aatcgagatt
    23821 tgttttccct ttagaccttc ttctaatcga aaagtcgtca aatacgaacc aaccggattg
    23881 tctttagcag ctgaaaagat cccttccgcc tctttctcag ggcttgtcgt gaaaggggga
    23941 cggcgaaata catgtccctc ccacggatac atcgtattga tgtaatggat cttgtcataa
    24001 tttgctaatt caatatgact aggaacagtg atttcatcaa aagaacaggc atcaaaatct
    24061 tttttataaa atgtttttgg tcgtaccgaa ggatttggcg aataacaaaa acgccatgat
    24121 ccatttaaag actgtttaag ggaggatgtg gtttctactt cttcccttgt tgcataacaa
    24181 acatgatcgc tatgtgcatt tgattggttc actctaaaaa cttgtggatc atctaaccat
    24241 tttatatttg ctttcatact tttttccttc tctctcataa ctttttgaaa tgaagcagtt
    24301 ctgtttggaa atcaccttga aaatcgggga agtaaagtcc aactgtcatc aattcggccc
    24361 ccgaaaatat ccgcttcgag gtctgttcct gataaagtgc ctcatcatct aaccccatca
    24421 gatatacttc atggaacgct ggttgagctg aagctagtat cctccctaaa aacacaatag
    24481 cttcatcttg tttaggagac acaaacatcc atgcggcaat attttcttca aaaggactcg
    24541 ccaaacgata ataatggcca tactggatca ccggacgaat cgtttgatag gatataattt
    24601 ggtcttttac ttgctttttt tcttcaggag acagctttgt caaatccagt tcataaccaa
    24661 aaacagcgct catagctgtt tctcctcttg ttttcaacga agtagatctg tcggtctgat
    24721 gatttggtac agctgataca tgagctgtga tggacgaaat cggataagca aggcttgttc
    24781 catattggat tttcatacgc tccactgcat ccgtgttatc acttgtccat gactgcggca
    24841 tataataaat aaacccggca tcaaaccgtc cacctccacc agaacagcct tcccataaga
    24901 tttccggata atcagttgtc aaatgttcaa gtagttcata aagcccgagc atataacgat
    24961 gcgctacttc tccttgtctc tgtgggtcaa gggtaatcga atagacatct gaaagcgaac
    25021 ggttcatatc ccatttgatg tagtcaagag gaatcgtatc taaaatcttt ctcatttgtt
    25081 tctcaatcgt ctgccgaaca tctaaccttg taaaatccag tacatgttgt gatctggaag
    25141 cagaaggcat tctacctggc tcctgcataa gaaaatcagg atgtgttcga tacagttcag
    25201 aatcaaccga gatcatttca ggttcaaacc ataaaccaaa tttcaatcct ttgctatgag
    25261 cataatctgc tatttcacgt aatccatttg tcaacttgcc ttcatactcg aaccagtctc
    25321 ctaatgaaga attgtccgca tctctttttc caaaccagcc atcatctaat acgaacattt
    25381 cgatgcctag ctcctttgct tcatcaatga tcgccttgat tttttcacta ttgaagtcaa
    25441 agtaagtggc ttcccagtta ttgaccagaa tcggccgttc agcaaattga tgacgacctc
    25501 ttgcgattct ttcacgtagc aactcatggt ggatctgact cattttgttc aatccctgac
    25561 ttgagtaaga aaggatcatt tcaggtgttt gaaaagactg gcctgcattc aattcccacg
    25621 aaaaacgatg gctgttgatc ccaccgacga ctctcaattg attgatctga tctttttcaa
    25681 gttcgaaaga atggttgccg gaataaacaa attgaagtcc gatcgcttct ccagtatttt
    25741 ctgtcgtatg atgatgaact aaagcaataa agttattcat atggtgactg ctcgttcctc
    25801 gacggctttc aaatactttt gttccagata aaacagattg ccgtgaaatc tgtctttcat
    25861 tgacatgtgc tcctggcaaa gcaatgactt catcaaatcg cctagtatgg gcaaagtcta
    25921 gctggaaact agccgctttt tctagtttga ttgtttctcc ggtttcattt cttatcttta
    25981 cagatcgtgt gatcactgct ctatcagtaa atatggtata gaacaaatcg aaataaatcg
    26041 ctgtttctct atccttaagt gaaatcacca acgtttgtgc ctctgacttg tccatcacat
    26101 aagtttgagg caagccttcg atctctggct tcccatctgt aatataatgg ctgtcatatc
    26161 tcagatccag taagtctgat ccattcttgc gctgaatgat cgatgcgggc gttcgaaagt
    26221 ccatgctgcc atgagaagag tattcttgcg gtagtgtatc tttcgacaga gaccggtcag
    26281 gatttaaggg aacatttcct gaaaatccac gatctcttcg gggataattt ttattgtctt
    26341 tgtactgctt gactggtttg ccaaaataaa tatgcgccaa tacattgctt tcttctatct
    26401 ttatcagata agaaagaaat gtatttcgta aatggaagac tcggctcttt tcatcaaata
    26461 agatgaaatt tttatctgtt tttctatgcg taatatccat catttcactc ccaacaaaac
    26521 ggaaaccgtt tacttttttt tataattcct gtatactgtt tactaaacaa ttttgtcaat
    26581 aaaaattttt tatttttttg aggagtgtgt aaaaatggtt ggaattagag atgtcgcacg
    26641 aaaggcaaat gtctctcctg ctacagtttc ccgtgtgttg aataacgatc agacgatcca
    26701 tgtggctcag actactcggc aacggatagt agaggcagct gctgcgttgg attatgacat
    26761 cagtactcgt aaatatatca aaaagagaat gccttcgatt ggtgtgatct ctacgatcgg
    26821 caaagcgagc gaagaacagg atatctatta caaacatttg cgagtaggga tggaagaaga
    26881 agctcgacgt ctgcatttag ggatgaatcg aatctataat ctatctgata atcctaaaca
    26941 atgggaagat ctagatcagt taggtgcggt gatcgttgtt gggactgtta cgaaagaatc
    27001 gattaccgcc ttgttaaaac aaaacatgca aatagtcgtc gtggataacc ctgacatcca
    27061 gcaagaggtc gatatggtgt atgcagatct tgaacggatg actaaaaccg tactggaatt
    27121 gttcttagaa aatggtcata aaagaatagc ctatatcggg ggatataaag tcgatatgga
    27181 tgaattcggt gggaagcaga tttctataaa tgaaaagagg atgcgtgcct atcagcattt
    27241 tatgatcgat catcacttag aaaaatatat gtcttatcat ctaggtggct ggacagaaaa
    27301 acaaggtgag gtacttgcga ctaatctatt agaaaatgaa tcccctcttc ctactgccat
    27361 tcttgtcggg agcgatccaa tggcaattgg tgtctatcgg gcattgaaac aaaaaaatat
    27421 ccaaattgga gaagagattg ttgtagcaag ctttgataat atcgagacaa tcgcaaacct
    27481 ctcccctggc ttgaccagtg ttcaaatcaa tgcgaaatca attgggcaag cagctgttcg
    27541 aatggcattg gagcgaattg acggtatacg ggacgaacca atcattttga cttatcctgc
    27601 acgattgatt gttagggaaa gttttatccc aagaacaaaa aggtattgaa agagaggttg
    27661 tgaaaagccg cttcgtttct tcatactcaa aacgaaacgg ctttttcaca acctcttact
    27721 tatacttatt cacaaaatcc aatgtctgct ggtaacaatt ttgcgattct tttagcgtgt
    27781 aatcaaattg gtattcatgg gtaatctctt tttttgtatc tgtataaaac aaggattgca
    27841 cgggtacctt taattctgtc aaccgatttt ctaatgcttg accttgctcc tggaatgagt
    27901 atgcatttcc gtcagttata tacgttggcg gaaattcctt agaaacatgg tcagccacac
    27961 tagcttcttt caattgctca ctcgttttcc aatcttttgt tccaatcaac gaccacgcaa
    28021 cggttttgac gaaaaacttc ataaatcgat catccgaatg ctgctgtgct gttagcttca
    28081 aatcaacagg tccgcaataa gaaatggttc ctttcagact atcttttgga agtagctgtt
    28141 tgattttcat ttgtttagca tacgcatcat tcgtttgtat agctgcatat tgcaatgcga
    28201 tttgtgaacc tgcactatct ccaccaaaaa ataattgttc catgtctagc aatttgtcgt
    28261 ctttttcttc agctaataaa tctttgatca gttcattcac ttgaagaact tgattagggt
    28321 attctgaatc aggcgctaat tcataattca tcgcaaccac tgcgatgttg gcgtctgaaa
    28381 ctaattttgt cgcaaattct ttgacacccg atttatctcc gccgacgaat cctccaccat
    28441 gtacccaaaa tagaacagga acaggcccct tgctgttttt tggatagtag atgtcataag
    28501 tattctcttt atacttggac tcatacgtca aatctgtttt tttcgccacg tttttttctg
    28561 cttgatcgaa cgttttttta tcagtgattt gaacagtatt tgaaaacata tggttgatca
    28621 agaatgctcc aggtctgggg gaaagctgga aggcaagaaa gattgcaaac gcgatcccaa
    28681 gaatgattcc gaagaaccat ccgataaatt taagaatttt ctttttcata aggctccctc
    28741 agtagttagt attaagcatt ctgattatac catagtgtta tttcctatca agctctccta
    28801 gatgatttaa tgattttccc caaacttctt ctgcaatgct tttgacaaaa gaaatttttt
    28861 ccattgttct tcaaacaaaa cattttcttc ttctgttgaa gcaaatccac attgaggact
    28921 taagcacaaa cgatccaaag gcagatatcg tgtcgcttcg tagatccggt caatcacttg
    28981 ctggtgctct tccatttcgc catttttcgt tacagtcaaa atctaagaca actaatttat
    29041 cttcagagac tttctctaaa agaacaaaac tcccagcacg ttcactatcg tattctaaat
    29101 aataggcacg gacattttct tgagtaaaat aatgggtctg ctacgtcgtc atcccccctg
    29161 atgccgccaa aatcgaataa taatttccgc gtcaaacatg tatatttagt gttgaatcat
    29221 ttgagaatat gaaaataaaa atctttttga tcaatcagtt aatcaaattg aaataacgaa
    29281 aaatctgaga cagaatgacg ttcattctga cccagatttg attttgcaca actcaacagt
    29341 ccttattggc aaatcgccaa aataatgtag actaactaaa aagactaaga aacaataaca
    29401 aaagttaaca cttcttagtc tttctttagc acgtctattt attaaagatt ttagggcctt
    29461 ttctcatgtt tttacgatca ttcttaggtg tttccatttg tttggatcgg cctccacctt
    29521 gtttcttttt gatcaattct ttcatttttt ctttttggtc catttttatt ctcctttaac
    29581 gaatgaagcg actaattcga tatgatgggt ttgcgggaaa agatctacag gctgaacttt
    29641 cttcacggca taacctgctt ctgcaaataa cttagcatct cttgcaaaag ttgccggatt
    29701 acatgagata tagacgattc gttctggtga catttcgatt gctgattcaa taaaatgatg
    29761 atctaatcct tttcgtggag ggtcaactac gatcacagat ggtttgatcc ctttttcttg
    29821 ccattttttc ataacttttt ctgcttttcc gacttcataa tgaacatttt cgattttatt
    29881 atttaaagca ttgaactgtg catcttcaat tgcttctgga ataacttcca tcccatacac
    29941 cttatccact ctgtctgcta aagacaagcc aatcgtacct attcctgaat aagcgtcgat
    30001 cacaatgtct tttttatcta actcggcaaa ctcgatggct gtttggtaca gatactctgc
    30061 ttgttgagta tttacttgat aaaatgactt tgctgagatt cgataggttt ttcctaatag
    30121 ctgatcttcg atatagtctc tgccatataa aactttttct ttgtctccta aaatcacatt
    30181 tgttttttct tcattgatat tttggatgac cgaaaccact tctggcaatt cttcatgaat
    30241 agcttcagca atttccttgg atttaaagaa tttttcttta cgagtaacta aaacgaccat
    30301 catttcatgg gaataatgac cacgacggat aatgatgtga cgaagctgtc cttcattttt
    30361 ttcttcatta tatcctcgta cttggaaccg ttgcaagata tctctgacta ctttgattgc
    30421 ttgatcgatg acaggatctt ggatataaaa atcttcgata gggactagat catggctatt
    30481 ctttttgtaa aaaccggtgg ctaactgtcc atcgatacgg cgaacaggga tctgtgcttt
    30541 attacggtag ccgaatggtt ctgccattcc gaccgttgga agtacaggaa cttctggcat
    30601 cttagcaatc ttcgccatta catgctctac ttgtttttgt ttgaagacaa gctgttgttc
    30661 atacttcaaa tgggctaaag gtgcgatacc tatgcgaagt aatgcattgt tttctacagg
    30721 ctgacgatca ggtgattgtg taataaatgt ttcgactttt ccatatccaa acttattgcc
    30781 tgcttttact actagtattt ctacttcttc tccagggagt gcattttcga taaataatgg
    30841 ataaccatcg atttttgcta cccccattcc ttcataactc aagtcgacga tttcgactgt
    30901 atatcgttcg tttttcttta ctatttgctg ttctgtcata ttttgctcct ttaagcctat
    30961 ttttcaatcc tactgaactc tactataaat cagctaatta cacaactaaa accggcactg
    31021 accttactac gttcagcacc ggcttttgat taattctttt gattaggtgt ttcgtctgct
    31081 atttttccgt caccgtcgat atcttcatcc gttaaacgtt cgacttcttt tacgaattct
    31141 ttgctgacaa cctctaattc ttcttcatct gtacctgtga ttgcttcatc aggaatctga
    31201 tcggtattgg caaagaactc aatgtgttga tgaagatttt ggaaatgcat cggtgcttcc
    31261 ccgccgtatt caccatctaa gttgatcatt actggtcgat tgatatcatc ttctgtttca
    31321 acgaacaaac tgcttgtttt ggtatagatt attcttggat catcgacatg tcgaccgcca
    31381 ttcagcatga gtgctgcaat atgcaagatc tcaaagatat ttgctgtctt cacaatgatt
    31441 aaagaaaact tcccatcatc caatttggca tctggcgcga tctgttcgaa tcctccaaca
    31501 gagttcgtca gccctaggaa aaacatcgaa gcatttccga tatattcccc ttcatcatac
    31561 ttcaagcgca ttttgattgg ctttactcga gggagcatct ctgctccttt cgcaagataa
    31621 gcaagataac caaaaatact ctttagttct gatggaactt cataagtcaa ttctgtcaaa
    31681 tagccacctg cagcgatatt gatgaagtaa ctgttatcag aaaggccgat gtccattttt
    31741 acggtttggt gtttttttat cacttctgct gctgctttaa tattatctct aggaattttt
    31801 aatgctcgag catagtcgtt cgttgtacca gctgggatga tggccatttt aggacggtgt
    31861 tcaagcggag cgattccatt aaccacttcg ttgattgtgc catcgccacc agctgccact
    31921 actagatcaa aacctagttc tgcgactcta catgcttcat tcttggctga atctggttct
    31981 ggtgtagttg cataggcgct ggcttcaaat cctgcttctt ctaatgcctg taggatatcc
    32041 gccaaatttt ttttcaataa ttcttttccc gaggtggggt tataaattac gcgtgctttt
    32101 ttcattttca gcctctatcg tttcgctaat tcatctttaa gtagcttatt cacgacacca
    32161 ggatttgctt gtcctttagt tgctttcatg atttgaccga ctaggaagcc aaccgcacga
    32221 tcttttccat ttttgaaatc atcgacagat tgttggttgt tatccaaaat ttcattaatg
    32281 attggcagta gtttgttcgg atcagataat tgcacccaac catttttttc aactacttct
    32341 tgggcttctc cgccatttgt gatcaattca cggaagactt ttttggcgat ttttgagctg
    32401 atcgttccat cggcgatcaa acgaatcatt cctgctaagt tctcaggtgt caattttgtc
    32461 tcagctaatt ccagtttctc actgttcaag taagcagaaa cttcacccat caaccagtta
    32521 gatgcttgct tggcatctgc accattttcc aaaacagctt caaaaaaatc agacatttct
    32581 tttgtaagtg tcaataccat tgcgtcatac tctggaagtc caaactcacg gatatagcgt
    32641 tcgcgacgag aagctggcat ctcaggcaaa ctagctttta ctttttgaat ccattcgtca
    32701 tcgatcacga atttcgggat atcaggctct gggaagtaac gataatcact agacccttct
    32761 ttgacacgca tcaaaactgt tttattcgtt gtttcgtcaa aacggcgtgt ttcttgttgg
    32821 atttctcctc ctgaaagaag aacttttgct tgacgttttt cttcaaaagc taaacctttt
    32881 ttcacgaaag ataaagagtt caagtttttc aattctgctt tcgtaccgaa ctcttcttgt
    32941 ccataagggc gaagagaaat attggcatca cagcgcatag agccttcttc cattttcaca
    33001 tcgctgactt ctgtaaattg gataattgat cgcaatgctt ctagataagc gtatgcttct
    33061 tctggtgaac gcatatcagc ttcagaaacg atttcgatca aaggcgtgcc ttgtcggttc
    33121 aaatcaacgt aagaataacc gccaatacca tgcatgtttt ttccagcatc ttcttctaaa
    33181 tggacacgtt cgatccggat ttttttcttt ttaccttcta cttcgatttc gatccatccg
    33241 tcatgaccga ttggttgatc aaattgggaa atttgatatg cttttggatt atctgggtaa
    33301 aagtaatttt tccggtcaaa atgagtatct ttagagattt cacagtttaa tgccaaagct
    33361 gctttcatcc cgaattccag cgcagcttta ttcattactg gcagcacgcc tggataaccc
    33421 cagtcgatca cgtttgtatt gctgtttggt tctgcaccaa aatgagctgg cgctggagag
    33481 aaaatttttg agttggtttt taactcgacg tggacttcta gtccaatgac tgtttcaaaa
    33541 ttcattcgtc tttccctcct aaaatagctg gttgttgttt atggaattct gttgcttgtt
    33601 caaacgcata tgccgcttga tacatcgttt tttcatcaaa ataattaccg atgatctgca
    33661 atccaactgg taatccttct gataaacctg ccggtacaga catacctgga agtccagcca
    33721 agttgacagg gatcgttaag atgtcactca tgtacattgt gattggatcg ttaatgttct
    33781 caccgattcc gaatgcaact gttggtgaag atggcccgat gatcaagtca taatctgcaa
    33841 agactttttc aaaatctcgt ttgatcaatg tacggacttg tcctgctttt ttgaagtaag
    33901 cgtcataata acccgcgctt aaggagaatg tacctaacat gatacggcgt tttacttctt
    33961 caccgaatcc ttctgaacgt gagttgacat atacatcatc taatgatttc acattttcag
    34021 agcgataacc gtagcggata ccatcgaatc gttgcaaatt ggaacttgct tctgatgagg
    34081 caatgatata ataaacagcc acaccgtatt ttgaatgagg caagctgact tcttccacaa
    34141 cagcacctaa tgaacggaat gtttctgctg ctttcaatac agcttctttt acgccagggg
    34201 ccacaccttc gcctaagtat tctttaggta aagcaatctt cattcctttg atgtcgcctg
    34261 tcagtccctc tgcaaaatca ggaactgaaa cgcctgaaga agtaccgtct ttttcgtcat
    34321 aaccgctgat agctgtcaat gctaatgcat tatctttcac cgtacgggtc attggtccga
    34381 tttggtcaag agatgaagca aaggcaatca aaccaaaacg tgagacacgg ccataagtag
    34441 gtttcatccc tacgatgccg ttaaaagctg ctggttggcg gatacttccc ccagtatcac
    34501 tacctaaaga aactggaatt tgtccagctg caactgctgc tgcagaacca ccagaagagc
    34561 caccaggtac ttttgttggg tcccaagcat tttttgtttt cttgaaataa gaagtttctg
    34621 tacttccacc catagcaaat tcatccatgt ttagcttacc aacagggatc atatctgctt
    34681 gatagatttt atccatgacc gttgcatcat aaattggttc aaaattgtaa aggatcttag
    34741 atgcagcggt tgtcaagata tcttttgtta cgatattatc cttgatacca attggaatac
    34801 cagcaaggac gttgctttca tcaatccctt tttcatctaa agcttttgcc tgttccaatg
    34861 ctttttcttc gttcaatgta atgaatgcct cgacctcagg ctctgtttct ttgattcgtt
    34921 caaatgttgt ttttgtcaaa tcttgtacag tgatttcttt cgatacaagc atatgatgca
    34981 gttcttctag agaacgattg tgtaattctg tcattatgcg ccagcctccc cattatcgat
    35041 gattgcgggt actttgataa agccgtcttc tgtttccggc acatttttca ataattcatc
    35101 gcggttccat ccagcttgcg cccgatcttg tcgcatcacg tttactgttt caaccacatt
    35161 cgacgtaaat ggaacacctt ccgtatctac ttcttccagc atttcaacca tatcgatgat
    35221 tttacctaat tggtcggtaa aacttgcaag ttcttcatct gcgaacgcaa gttttgctaa
    35281 tttggcaacg tgtttcactt gttcttcact aatcgccata ctaccccttc tttctaaacg
    35341 agttaaaaac tttgctctct attttaacac aagagtgccg tgtctgaaat tgtttattga
    35401 aatttgtctg aaaaaactca tttttcattc ttctactgag cgacttttag ccaatgcatc
    35461 tgccatttga tcttctgtcc aaacttcgat tcctaattct tgtgctttgg tcagcttgct
    35521 gccagcatct tctcctgcta ccacgatatc cgtttttttc gatacgctac cagtcacttt
    35581 cccgccaaga ttttcgatcg tctcttttgc ttcttcacga gtaaatcgtg ttagtttacc
    35641 agtaaggact actgtttttt ctttaaaagg agactcgact tcttgcaatt gggcatttcg
    35701 aagccctttg tattcgaaat tgacgcctgc ttgttttaat tcattcatca attcatggac
    35761 ttcttcgtta ctgaaataag tgactacact gtcggcaatg atatcaccga tcgtatcaag
    35821 cgcaataatc gactcatagt cacttttact caatgtttct agatcaccga aatgttctgc
    35881 taaaattttt gctgctttcg ctccgacatg tcggattcct aacccgaaaa tcaaccgctc
    35941 gaccgaatta tctttgctgt tgtcgattgc tgtcaaaata ttatttgctg atttttcttt
    36001 tattttgtcc aacgtcaata actcttcttc tgtcaatttg taaagatcag caacatctgc
    36061 tactagtttt ttatcataca tctgttctag tacgcgcggc cccaacccat cgatattcat
    36121 cgcatttctt gaaacaaagt ggttcaaacc ttctttcatt tgagcagggc atttaggatt
    36181 gatgcaccgt aaggctactt cttcatccaa atggacaagt tcacttccac aaactggaca
    36241 atgtgtaggc aattggtatt cttcgctgtc tttaggtcgt ttatctaaca cgacttgcga
    36301 aacttctggg atgatgtctc ccgctttata aatcagaact gtatctttca agcggatatc
    36361 cttcaactta atataatcac cgttatgcag gctggcacgg cttactgtcg ttcctgctac
    36421 tcggactggt gtcatgatag ctgttggcgt tactacccct gttcgaccga ctgtccattc
    36481 gatgttttcg attagcgttt caacttcttc tggcggaaac ttgtaagctg ctgcccatct
    36541 cggtgctttg atagtaaaac caagttgatc ttgtagcgag aattcattca ctttgatgac
    36601 gattccatcg atttcatagg gcaaatccac acgtttgtca tgatattctt caatgtatga
    36661 ccacacttca tcaatcgaat gacatagacg tttttcccga ttcgtgtgga atccaatttt
    36721 ttctagctct tctagcgcat catactgggt ttttgcttgc ataggaccga aatcagctac
    36781 tgtgtataaa aaagtgtcca gatttctttt cgctgtgatt ttagaatcta actgacgcaa
    36841 agaaccagct gcggcgttcc gaggattcgc aaagatatct tttccctctg cttctctgtc
    36901 ttggttcaat tggacaaatg accgttttgg catgaaacat tcgcctctta cttcgatgtt
    36961 cattggttct tttaatttga ttgggaccga ccggacagtt tttaggtttt cagtgatgtt
    37021 ttctcctaca gttccatccc cgcgagtcgc acctctcacg aaatttccat cttcataacg
    37081 caaagaaacg gaaagtccat ctatttttag ttcacagcaa tagtcaacta cacgtcccac
    37141 tgctttttgt acacgttgat caaaagctat cagctcttct ttgctgaaaa cgtcgttcaa
    37201 actgtaaagc ggaatatcat gagtgacttt ttcaaaacct tctaatactt ttccgcctac
    37261 tcgttgagtg ggagaatcag acgtgatcaa atcaggatat tgttcctcga ttgctactaa
    37321 ttctgcgtat tctttatcat aaacatagtc ttccactgtt ggtttatctt ctacgtagta
    37381 ttcgcgagac cattggttca gtcttgtacg aagttcttct gctcgtttag cagcttcgtc
    37441 aatcgtcatt tcatgttcca tcatttaccc tccactacta agctttttca attggcgcaa
    37501 aggcagccaa aagacgtttt actccttgac ttgggaaagc cacatctagt tctagatcct
    37561 tcgacgtccc gcttactcgg acgactgttc ctagacccca ttttttgtgt ttgactttat
    37621 ctccaggttt ccagctgtta ttttcccctc cagtagcact tttagaggga acaaccgctg
    37681 tctctggctg tttatagcta tatgctgttt ttttagcgaa tacaggcgtt tttttaggcg
    37741 taggacgcat gccttcgatt tccagtaact cctgatcgat ttcttctaca aaacgagaag
    37801 gtcggttata ttgcgtccgt ccatataatg ttctggagaa agcatttgtt aaatacaacg
    37861 cttcttctgc tcgcgtgatc cccacatatg cgagacggcg ttcttcctcc agttcgcttt
    37921 cttccatcaa tgctcgagat aaaggaaaaa ccccctcttc tagaccaatc aggaatacaa
    37981 ctggaaattc taatcctttg gctgcatgca aagtcatcaa tgtcacttgg gaagcatctt
    38041 cttctaaatt gtcaatgtcg gaaacaagtg ccagatcatt taagaagacc gttagttttt
    38101 cttctggtgc atcagcgtct tcttcatttt gctgttcgaa ttgtttgtcg aattcttgtg
    38161 tgaccgttaa aaattcctcc aagttttcga tgcgtgcttg tgcttctaat gtattttgga
    38221 tttttaagtc ttctagatat ccgctacgat ccaaaacctc tttcgtcaat tctgtcactg
    38281 tcaagtatgg gatcatctga gtgacttctt ggatcatctc tccaaatgca cctaattgct
    38341 gtcctgcttt tccaccgata tttgccagat ccacattttg cgctgcttca agcagtggcc
    38401 attcatgaag agaagcaaaa ctgcgtagtt tttcgataga tcctggtccg atgcctcgtt
    38461 tgggcgaatt aacgatacgc tcaaaactga ttgaatcttg aggatttacc agcacattca
    38521 aatacgctaa aatatctttg atttctttac gatcgtagaa cttgtgccct ccaaccattt
    38581 tataaggaat atttgctttc aacagagttt cttccattac acgagattgt gcgttcgtcc
    38641 ggtacaagat ggcaaagtcg ccgtaattcc gatgattgct tcgaatctct tcttgcatcc
    38701 ggtctacgat aaaacgggtt tcgtctcgtt cattatctgc acggtagtaa gtgatcttgt
    38761 ttccttcttt gttttctgtc cacaagtttt ttggttttcg attgctattg ttttcaatga
    38821 cttggtttgc tgcgctaagg atgtttttcg tggagcgata gttttgttct agcaaaatta
    38881 ctgcagcatc tggataatct ttttcaaaat ccaagatgtt ctgcatatct gcaccgcgcc
    38941 agccgtaaat actttgatct gcatctccca cgacgcacag gttcctaaat ctacctgcca
    39001 ataaattgac tagcgtatac tgtgcgtgat ttgtatcttg gtactcatct acatgaatat
    39061 aatggaattt gttttgataa tacgtgagtg aatcaggatg ttcttcaaat aatcgaatcg
    39121 tattcatgat caggtcgtca aagtccatac attgattata ttgaagctct ttttggtaag
    39181 cagcataaca ttttgccgca atctcttcgt aataactgcc ttgcattttt gcaaaatcct
    39241 gaggggtctg caggctgttt tttgcttgag agattgttcc taatatactt cgaggatcat
    39301 attttttcgg atcaatgttc agttctttta gtatccgttt catcaaggtc agctgttcag
    39361 aagaatcgat gattgtaaag ttccggttat acccgataaa atcaacatct cttcttaaaa
    39421 tacgcacaca catcgagtga aaagtcgata cccagacatc ttcgcctcca gatgcgagaa
    39481 tcgcattgac acgttctttc atttcttttg cagccttatt ggtaaaagta atcgctagga
    39541 tgttccaagg attgacttct ttttcctcaa tcaaataggc aatgcggtgt gtcagcactc
    39601 tcgtctttcc acttcctgca cctgccatta gcagtaatgg tccgtcagtg tgaagaacag
    39661 cttctttctg acgggggttc atcccgttta ataattcagc tttggatgac attaacaaca
    39721 tcctttcttt tatcaatctt gttaattata gcattttctt ggctttcatt aaactagcta
    39781 gttcaaaatc atttgtttct ttttatggat ttttccttgt caattcgttg atttcgcgct
    39841 aaattaaaag aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag aaattagata
    39901 agactcagat atattttatt ctaaaaggaa tatttgttgg actgctgaca ggaatcgtcg
    39961 ttagcctgtt tcgattatct atcgaaaagc taagcgattt agttcgaagt atctatgaaa
    40021 tgagtcggga acagccgatt tacttaattg gcctagctat ttgctgcatc cttgcagcat
    40081 ttttcgtggg ttatctggtc aaaaatgaac cggatatcaa gggaagcggg attccacaag
    40141 tagagggaca gcttcgcggg gaattatcaa tgaactggtt ctctgtttta tggaaaaagt
    40201 tcattggcgg tgtactttca gttggtgcgg gcctttttct aggtagagaa ggaccttcta
    40261 tacagcttgg cgcatccatt ggacaaggag ctggacaatt gtttcggagt ccatcttcag
    40321 aagaaaagat tttaatttct agcggtgcaa gtagcggact tgcagcagca ttcaatgctc
    40381 ctatcgcagg attactcttt gttttagaag aaatccatca ctccttttct ccacttgttt
    40441 ggctgacatc atttgcttca gcaatcactg ctaacttcgt ctctctttac tttttcggct
    40501 tgagacctgt tttatatcta ggtgaccttc cttctttgcc gttgaaatat tatggttcct
    40561 tagtgatctt aggagtactt ctaggcatct taggttttat ttatcagaat gtcttgctgg
    40621 ctttgccaaa attttacaac cgattgcctt taccagctta ttttcacggg ttcgttcctt
    40681 tcttgctgat tttgccaatt ggttatttat ggcctagtac tctaggcgga ggcaatcaaa
    40741 tcgttttagc ttttggacat gcttccttgc caatcattat gattatcggt ttgtttgttc
    40801 ttcgattcgt tttttccatg atttcttacg gtgggggtct tccagggggg attttccttc
    40861 cgatattgac attgggggct ttaatcggta gcttttatgg aaacattatg gttgatttcg
    40921 ggatggatcc aatctacatc aaggattttg tcgttattgc catggctggt tatttcacag
    40981 cgattggaaa agcaccgctg acagcaatta ttttagtgac agaaatggtt ggctctctta
    41041 atcatctgat gcctttagga ttggctgctc ttgttgctta tatcgtcaat gaccttctcg
    41101 gcggtaatcc aatttacgaa tcattattgg aacgtctact tggaaatact cagaccgcct
    41161 cactaaaagg tgcaaggaca acttttgatt ttccagtaac ggcagagagt tctttagacg
    41221 gattaatggt acgagacttc aattggccta aaaatatgct gctaatctct atacgcagag
    41281 gaaatcagga aatcttgact cacggtgata cgatcatgaa tgtaggagat attttggtta
    41341 tcttgactga tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct aaatcttcta
    41401 tcacaaattt gaagcaatca ttttaacgaa catggaaaaa ctattttaca acaactattc
    41461 atctacacta cttgattcta tatgattcta taaaaaaaga agaggctggg aaaacccagc
    41521 ctcttctttt ttatttctat gcttctgcta ctgcaggagc ttttctcaat aaaccataaa
    41581 tcacaccaga tacgactgct ccgatagcaa taaacaccaa atacatgacc gggtgactca
    41641 gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa tttgattccg aaagctccga
    41701 ctaatccgcc agttaaagca gaaccaataa taaaacttgg aatggcacga atcgggtcag
    41761 ctgctgcaaa tggaatcgat ccttccgtga caaacgatag tcccatcacg atgttcgtca
    41821 aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta gcaacgaaaa
    41881 ttgccaatgg aggtaccatt cctccagcca taacagaagc catcacgaca cttccgccag
    41941 ttgcaacagt cgtagctaaa gttgctgtac caaacacata agcggctttg ttgattggac
    42001 cgcctaaatc tgccgccatc attccagcta ataatgcacc aagtaaagca gcattcgaac
    42061 cactcatact tgtcaagaaa ccgttcaatg catcattgat tgctttcata gggacattga
    42121 ccagcaacat caagaaacca gtaatcagta agccaaacac tggataaaag aggatcgttt
    42181 taattccatc caatgatttt ggtaagttct tgaataattt acgtaaaaat acgatgacat
    42241 aaccagctaa gaatccacca atcagtgcac ctaagaatcc tgcaccgcct gtattagcta
    42301 atgcaccagc agcaaatcca acgatcaaac cagggcggtc accgatactt gaagcaataa
    42361 atcctgctaa taccggcagc atgaaaccaa atgcagcttg tccgatttgg ttgaaccagc
    42421 tagcagcttg attgtaattc cctagatttg ataattgatc atgtggcacg ccaataaatt
    42481 ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc aataacgaaa ggtaacatgt
    42541 gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca tccgcatttc
    42601 cttcttctga actgctttct tttccatcac catggaatac tggtgcagta ccactaaggg
    42661 ctagattgat caactcttct ggtttacgga taccatcact gacaggacga ttgatcagtt
    42721 cttttccgtc aaagcgattc atctctactt ttttgtctgc tgcaacgatt acaccttctg
    42781 cacgggcgat gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg ttcgtttcga
    42841 ctttgatctc cacacccatt tctttcgctt ttttcttcaa agcatcttct gccatgtaag
    42901 tgtgagcgat ccctgttgga caagctgtaa cggcaacaac gaattttctt tctggatgag
    42961 cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct ttttgctctt
    43021 cagctgtttg gaacaagtct tggacttctt ctggtgtatt tgcttctttt aattttccta
    43081 cgaaatctgc atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa tgtgtgtcgt
    43141 ttgcgccttc tggagctgcg atcatgaaaa acagataagt tggctgtccg tctaaggctt
    43201 cataatctac tcctttttgg ctttttgcga ataaaactgt tgcttcttta actgcactgt
    43261 ttttcgcgtg aggcatggcg attccatcac caagacctgt agaagtttgt gcttcgcggg
    43321 ctagaattcc ttctttatat gtttcgatat cggaaatccg gccggcatca aacatttttt
    43381 ggaccatttc atcgattgcg ccttttttat cagtcgcttg caaatccatg atcatcgctt
    43441 ctttgaccat taaatccttg atattcatga ttatcctcct tgaaactaat caatttttgt
    43501 gatttcaact tctggcagca actcatcaat caattcgcga gctgccaagt catcagaaaa
    43561 agctgttgcg cttccgcaag caactcccca tttaaaggct tcgattggat ctttcgtacg
    43621 gctgtaagcc ccaataaagc cagcaatcat tgaatcccct gcacctactg aatttttcaa
    43681 tgggcgaact aatacattcg ttttataagt tccttgttcc gtgaagagca aagcaccgtc
    43741 tccagccatt gagatcagca cattctgtgc cccttcagct agtaattttt tcccatacgg
    43801 aatgatttct tctacagatt ggaaggttgt gttgtaaagc tcagctaatt cgtgattgtt
    43861 tggtttgatc aacaacggtt tcttaacaag tgcatttttt aggtcatcac ctgttgtatc
    43921 aatcacgaaa tctgcacctt tttctcgaat gatatcaatc agttcttgat aaaatccttt
    43981 tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata tctccttgtc ctactgttcc
    44041 taatgcttgc ttcaactcat cgatttcttc atctgaaata gatgggccta agccattgat
    44101 ctcagtttca gtgtctgatt ttagtttgat attgattctt gtatcttctt ttacggttgt
    44161 gaacaaagac tggatttgtt ctttttcaag ccacttcgaa ataaattcgc cagtgaaccc
    44221 tcctaaaaac cctagtgctg tagattgaga atcgatccgt ttcaaaatac gggaaacatt
    44281 gatccctttt cctccaggta acttgaagtc ttccttcatt cggttcaaat cgcctagttt
    44341 taacccatct acgcgtacga tataatcaat tgatggatta agtgttactg tgtaaatcat
    44401 tgtattgcct ccttaattgt tgtcttttca cggaattcat taacgaattc taatggacaa
    44461 cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga caactggttg
    44521 aatttggtat gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc taaacgttta
    44581 acagctgcct cttctggatc cggagtcgtg aatccttgtt caagatgggc accgttcatt
    44641 cccataaatg ctttattaaa atgaaaattt tttagttgtt ctatcgcatt agatcctaaa
    44701 acagcattag ttgccagctt tatcgttcca cctaaaataa tggtagcaag ctccaaatca
    44761 actaaatttg ccgcatgttg aacggaattt gtcactactg tgatctgttt tccttgcaga
    44821 aaaggaatca tttctaacgt agtagagcca gcgtctaaat aaatgacatc ttcgtcatta
    44881 atggtttcag ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt gagtgatttt
    44941 tcactcatat tctgctcaaa attcaataat tgctctttct tagctccgcc atgaactctt
    45001 tctagaaacc cactattctc cagctcttgt aaatcacgtc ttatcgttga ttcagaagca
    45061 ttcaaaaggt ttacaagatc ttgtgatttc acgattttct gctgttcaag caagtctagg
    45121 attttttgtc tgcgttcctc tgtaagcatt ttcacgatca cctcaataca aataatagca
    45181 tatttttttt ctactagcaa tataaatcag tcaaaaacgt tcataaaaat tataaatcag
    45241 tcaaaaaaaa aataaaaaac cagtcagaaa gactggtttt ttattttttt gtctatcatc
    45301 aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac agaagcaggt
    45361 tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat gaccaaaaaa
    45421 ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga taataatgat
    45481 gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc atattttttg
    45541 taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca gatgctgctc
    45601 acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt tgaaggtaac
    45661 atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt ctgaaggaac
    45721 atagattctt aaggataaaa gatactcttg atatttttca tctgttacct ctacttctag
    45781 atattcccta atataataac aataagcttc gattccatta tccaaagcaa cagtcggatc
    45841 gataacttta tagcacatca tgcgtgatcg attctcgtca acccaaggaa aatacgcctg
    45901 agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgga tatcataact
    45961 ttttgttaca aaaaatgaag cattcttttt aggaacgggt tgcatttgtg cattctgata
    46021 ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg gtttcaataa
    46081 accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa aataggattg
    46141 aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct ggatacatat
    46201 gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa aaatcggttt
    46261 agggatgatt tgatgataat agcgactata atacatttcc caacacgaag tgaaacataa
    46321 accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta cttccccgtt
    46381 ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca atagccgttg
    46441 agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat aggcgaacac
    46501 cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct tttctttgaa
    46561 aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc gaaaactaca
    46621 ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg ctagcattat
    46681 tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag agcggataaa
    46741 tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct gttttttttc
    46801 ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc caatcatttt
    46861 ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga aaaactaaaa
    46921 ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa aagaaacaaa
    46981 gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa acttttcgaa
    47041 tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga tcgatttcta
    47101 aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt taagctcaaa
    47161 aaa
//
LOCUS       DACEMS010000008        44615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEMS010000008 DACEMS010000000
VERSION     DACEMS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.008"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(218..1015)
                     /locus_tag="IW735_000400"
     CDS             complement(218..1015)
                     /locus_tag="IW735_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1043426.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IW735_000401"
     CDS             complement(1012..2196)
                     /locus_tag="IW735_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1043427.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IW735_000402"
     CDS             complement(2221..3468)
                     /locus_tag="IW735_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1043428.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IW735_000403"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IW735_000403"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1043429.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IW735_000404"
     CDS             5448..5762
                     /locus_tag="IW735_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1043430.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IW735_000405"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IW735_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1043431.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IW735_000406"
     CDS             6447..7091
                     /locus_tag="IW735_000406"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1043432.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IW735_000407"
     CDS             7106..7435
                     /locus_tag="IW735_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043433.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IW735_000408"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IW735_000408"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1043434.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IW735_000409"
     CDS             8423..9247
                     /locus_tag="IW735_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1043435.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVELNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IW735_000410"
     CDS             9240..9587
                     /locus_tag="IW735_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1043436.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IW735_000411"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IW735_000411"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1043437.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IW735_000412"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IW735_000412"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1043438.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IW735_000413"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IW735_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1043439.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IW735_000414"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IW735_000414"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1043440.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IW735_000415"
     CDS             15244..16011
                     /locus_tag="IW735_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043441.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IW735_000416"
     CDS             16001..18043
                     /locus_tag="IW735_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1043442.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IW735_000417"
     CDS             18324..18473
                     /locus_tag="IW735_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1043443.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IW735_000418"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IW735_000418"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1043444.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IW735_000419"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IW735_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1043445.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IW735_000420"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IW735_000420"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1043446.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IW735_000421"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IW735_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1043447.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IW735_000422"
     CDS             22890..23972
                     /locus_tag="IW735_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1043448.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IW735_000423"
     CDS             23972..24775
                     /locus_tag="IW735_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1043449.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IW735_000424"
     CDS             24845..25585
                     /locus_tag="IW735_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1043450.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IW735_000425"
     CDS             25596..26600
                     /locus_tag="IW735_000425"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1043451.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="IW735_000426"
     CDS             complement(26645..28489)
                     /locus_tag="IW735_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043452.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28701..29219
                     /locus_tag="IW735_000427"
     CDS             28701..29219
                     /locus_tag="IW735_000427"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1043453.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29347..30030
                     /locus_tag="IW735_000428"
     CDS             29347..30030
                     /locus_tag="IW735_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1043454.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30339..30412
                     /locus_tag="IW735_000429"
     tRNA            30339..30412
                     /locus_tag="IW735_000429"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30373..30375,aa:Arg,seq:tct)
     gene            30571..30930
                     /locus_tag="IW735_000430"
     CDS             30571..30930
                     /locus_tag="IW735_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1043455.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31053..32684
                     /locus_tag="IW735_000431"
     CDS             31053..32684
                     /locus_tag="IW735_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1043456.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32746..33387)
                     /locus_tag="IW735_000432"
     CDS             complement(32746..33387)
                     /locus_tag="IW735_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1043457.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33669..33977
                     /gene="rplU"
                     /locus_tag="IW735_000433"
     CDS             33669..33977
                     /gene="rplU"
                     /locus_tag="IW735_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1043458.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34004..34348
                     /locus_tag="IW735_000434"
     CDS             34004..34348
                     /locus_tag="IW735_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1043459.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34361..34654
                     /gene="rpmA"
                     /locus_tag="IW735_000435"
     CDS             34361..34654
                     /gene="rpmA"
                     /locus_tag="IW735_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1043460.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35061..37895
                     /locus_tag="IW735_000436"
     CDS             35061..37895
                     /locus_tag="IW735_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1043461.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38086..38577
                     /locus_tag="IW735_000437"
     CDS             38086..38577
                     /locus_tag="IW735_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1043462.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38680..39669
                     /gene="manX"
                     /locus_tag="IW735_000438"
     CDS             38680..39669
                     /gene="manX"
                     /locus_tag="IW735_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1043463.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39702..40505
                     /locus_tag="IW735_000439"
     CDS             39702..40505
                     /locus_tag="IW735_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1043464.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40526..41440
                     /locus_tag="IW735_000440"
     CDS             40526..41440
                     /locus_tag="IW735_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1043465.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41523..41897
                     /locus_tag="IW735_000441"
     CDS             41523..41897
                     /locus_tag="IW735_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1043466.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42036..42281
                     /locus_tag="IW735_000442"
     CDS             42036..42281
                     /locus_tag="IW735_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1043467.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42453..43517
                     /locus_tag="IW735_000443"
     CDS             42453..43517
                     /locus_tag="IW735_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1043468.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43763..44188
                     /locus_tag="IW735_000444"
     CDS             43763..44188
                     /locus_tag="IW735_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1043469.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44181..>44615
                     /gene="nusB"
                     /locus_tag="IW735_000445"
     CDS             44181..>44615
                     /gene="nusB"
                     /locus_tag="IW735_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1043470.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKEL"
BASE COUNT        14172 a         7688 c         9364 g        13391 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gagttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    28561 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    28621 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    28681 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    28741 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    28801 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    28861 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    28921 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    28981 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    29041 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    29101 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    29161 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    29221 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    29281 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    29341 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    29401 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    29461 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    29521 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    29581 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    29641 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    29701 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    29761 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    29821 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    29881 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    29941 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    30001 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    30061 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    30121 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    30181 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    30241 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    30301 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    30361 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    30421 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    30481 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    30541 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    30601 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    30661 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    30721 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    30781 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    30841 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    30901 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    30961 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    31021 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    31081 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    31141 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    31201 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    31261 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    31321 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    31381 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    31441 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    31501 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    31561 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    31621 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    31681 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    31741 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    31801 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    31861 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    31921 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    31981 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    32041 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    32101 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    32161 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    32221 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    32281 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    32341 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    32401 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    32461 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    32521 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    32581 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    32641 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    32701 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    32761 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    32821 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    32881 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    32941 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    33001 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    33061 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    33121 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    33181 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    33241 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    33301 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    33361 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    33421 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    33481 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    33541 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    33601 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    33661 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    33721 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    33781 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    33841 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    33901 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    33961 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    34021 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    34081 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    34141 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    34201 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    34261 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    34321 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    34381 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    34441 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    34501 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    34561 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    34621 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    34681 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    34741 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    34801 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    34861 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    34921 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    34981 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    35041 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    35101 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    35161 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    35221 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    35281 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    35341 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    35401 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    35461 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    35521 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    35581 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    35641 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    35701 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    35761 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    35821 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    35881 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    35941 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    36001 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    36061 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    36121 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    36181 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    36241 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    36301 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    36361 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    36421 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    36481 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    36541 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    36601 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    36661 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    36721 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    36781 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    36841 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    36901 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    36961 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    37021 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    37081 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    37141 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    37201 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    37261 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    37321 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    37381 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    37441 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    37501 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    37561 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    37621 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    37681 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    37741 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    37801 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    37861 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    37921 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    37981 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    38041 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    38101 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    38161 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    38221 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    38281 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    38341 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    38401 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    38461 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    38521 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    38581 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    38641 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    38701 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    38761 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    38821 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    38881 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    38941 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    39001 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    39061 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    39121 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    39181 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    39241 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    39301 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    39361 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    39421 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    39481 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    39541 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    39601 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    39661 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    39721 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    39781 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    39841 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    39901 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    39961 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    40021 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    40081 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    40141 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    40201 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    40261 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    40321 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    40381 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    40441 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    40501 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    40561 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    40621 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    40681 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    40741 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    40801 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    40861 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    40921 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    40981 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    41041 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    41101 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    41161 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    41221 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    41281 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    41341 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    41401 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    41461 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    41521 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    41581 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    41641 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    41701 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    41761 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    41821 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    41881 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    41941 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    42001 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    42061 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    42121 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    42181 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    42241 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    42301 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    42361 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    42421 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    42481 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    42541 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    42601 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    42661 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    42721 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    42781 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    42841 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    42901 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    42961 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    43021 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    43081 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    43141 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    43201 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    43261 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    43321 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    43381 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    43441 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    43501 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    43561 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    43621 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    43681 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    43741 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    43801 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    43861 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    43921 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    43981 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    44041 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    44101 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    44161 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    44221 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    44281 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    44341 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    44401 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    44461 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    44521 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    44581 tcaacggtgt tttatccaat gtattgaaag aatta
//
LOCUS       DACEMS010000009        42839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEMS010000009 DACEMS010000000
VERSION     DACEMS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.009"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            149..433
                     /locus_tag="IW735_000446"
     CDS             149..433
                     /locus_tag="IW735_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1043471.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(714..1133)
                     /locus_tag="IW735_000447"
     CDS             complement(714..1133)
                     /locus_tag="IW735_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1043472.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1135..1719)
                     /locus_tag="IW735_000448"
     CDS             complement(1135..1719)
                     /locus_tag="IW735_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1043473.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1766..3241)
                     /locus_tag="IW735_000449"
     CDS             complement(1766..3241)
                     /locus_tag="IW735_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1043474.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3422..3733
                     /locus_tag="IW735_000450"
     CDS             3422..3733
                     /locus_tag="IW735_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043475.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3848..6184)
                     /locus_tag="IW735_000451"
     CDS             complement(3848..6184)
                     /locus_tag="IW735_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1043476.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6326..6994
                     /locus_tag="IW735_000452"
     CDS             6326..6994
                     /locus_tag="IW735_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1043477.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6997..8040
                     /locus_tag="IW735_000453"
     CDS             6997..8040
                     /locus_tag="IW735_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1043478.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8277..9158)
                     /locus_tag="IW735_000454"
     CDS             complement(8277..9158)
                     /locus_tag="IW735_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1043479.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9317..10045
                     /locus_tag="IW735_000455"
     CDS             9317..10045
                     /locus_tag="IW735_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1043480.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10201..10518
                     /locus_tag="IW735_000456"
     CDS             10201..10518
                     /locus_tag="IW735_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1043481.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10511..11119
                     /locus_tag="IW735_000457"
     CDS             10511..11119
                     /locus_tag="IW735_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1043482.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11174..11965
                     /locus_tag="IW735_000458"
     CDS             11174..11965
                     /locus_tag="IW735_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1043483.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12103..12591
                     /locus_tag="IW735_000459"
     CDS             12103..12591
                     /locus_tag="IW735_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1043484.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12642..13514
                     /locus_tag="IW735_000460"
     CDS             12642..13514
                     /locus_tag="IW735_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1043485.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13690..14052
                     /locus_tag="IW735_000461"
     CDS             13690..14052
                     /locus_tag="IW735_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1043486.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14061..14813
                     /locus_tag="IW735_000462"
     CDS             14061..14813
                     /locus_tag="IW735_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043487.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14827..16629
                     /locus_tag="IW735_000463"
     CDS             14827..16629
                     /locus_tag="IW735_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1043488.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16906..17947
                     /locus_tag="IW735_000464"
                     /pseudo
     CDS             16906..17947
                     /locus_tag="IW735_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(17999..20143)
                     /locus_tag="IW735_000465"
     CDS             complement(17999..20143)
                     /locus_tag="IW735_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1043489.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20500..21873)
                     /locus_tag="IW735_000466"
     CDS             complement(20500..21873)
                     /locus_tag="IW735_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1043490.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21873..22268)
                     /gene="rbsD"
                     /locus_tag="IW735_000467"
     CDS             complement(21873..22268)
                     /gene="rbsD"
                     /locus_tag="IW735_000467"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1043491.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22296..23213)
                     /gene="rbsK"
                     /locus_tag="IW735_000468"
     CDS             complement(22296..23213)
                     /gene="rbsK"
                     /locus_tag="IW735_000468"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1043492.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23321..24334)
                     /locus_tag="IW735_000469"
     CDS             complement(23321..24334)
                     /locus_tag="IW735_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1043493.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24415..25014)
                     /locus_tag="IW735_000470"
     CDS             complement(24415..25014)
                     /locus_tag="IW735_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1043494.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25200..25901
                     /locus_tag="IW735_000471"
     CDS             25200..25901
                     /locus_tag="IW735_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043495.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25911..28622
                     /locus_tag="IW735_000472"
     CDS             25911..28622
                     /locus_tag="IW735_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1043496.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29073..30701
                     /gene="treC"
                     /locus_tag="IW735_000473"
     CDS             29073..30701
                     /gene="treC"
                     /locus_tag="IW735_000473"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1043497.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30761..31942)
                     /locus_tag="IW735_000474"
     CDS             complement(30761..31942)
                     /locus_tag="IW735_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1043498.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32194..33717
                     /locus_tag="IW735_000475"
     CDS             32194..33717
                     /locus_tag="IW735_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1043499.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33786..34514)
                     /locus_tag="IW735_000476"
     CDS             complement(33786..34514)
                     /locus_tag="IW735_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1043500.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34527..35808)
                     /locus_tag="IW735_000477"
                     /pseudo
     CDS             complement(34527..35808)
                     /locus_tag="IW735_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(35949..36551)
                     /locus_tag="IW735_000478"
     CDS             complement(35949..36551)
                     /locus_tag="IW735_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1043501.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36796..37002
                     /locus_tag="IW735_000479"
     CDS             36796..37002
                     /locus_tag="IW735_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043502.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37314..38033
                     /locus_tag="IW735_000480"
     CDS             37314..38033
                     /locus_tag="IW735_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1043503.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38114..38797)
                     /locus_tag="IW735_000481"
     CDS             complement(38114..38797)
                     /locus_tag="IW735_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1043504.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39015..41201
                     /locus_tag="IW735_000482"
     CDS             39015..41201
                     /locus_tag="IW735_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1043505.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            41547..41957
                     /locus_tag="IW735_000483"
     CDS             41547..41957
                     /locus_tag="IW735_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1043506.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            41971..42327
                     /locus_tag="IW735_000484"
     CDS             41971..42327
                     /locus_tag="IW735_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1043507.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        13966 a         7532 c         8558 g        12783 t
ORIGIN      
        1 tcatgtttgt tgactattgt gacttgtgac cgaggagttg gcaggagaac accgtttatt
       61 cgaaaaaagg aactgcgaca agacttttgt cacagttcct tctttttttt gctatgctat
      121 actagatgta aaaaatagga gaaataaaat gagggaaatt tattcttttg aatcaaaaat
      181 ctatgcttcc gaagtcggca agggcggggc atatattatt tttccgtatg atataagaaa
      241 agaattcggt gctggaagag ttaaagtcca agcaactttt gacaatgtgc catatgaagg
      301 cagtatcgtg aatatgggcg tgaaaaatcc agatggttca gtctgctaca tccttggact
      361 acgaaaagat attcgtaaaa acatcggaaa agaaatcggt gatattgtag cagttacagt
      421 gaagcaaaaa taaaagaggt tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt
      481 tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa acccatgttt
      541 acaaaatttt tcttactact cgaaacgaag cggctttatc acaacctctt gataaacggc
      601 gttcaatcag ctgtccctcg gtattagctc aaaaacggca aaatatgaga agctattttt
      661 ccattttttt ctctcatact caggtcataa cgctgatttc acaacctctt ctttcataat
      721 ggtttttcac catctttata ataaactggt tctttgatat ggaaatctat tttttccaac
      781 caatctaaaa tcttgtaacc taactcttct gacatcggaa aagaggcgtt gatttgtgca
      841 ttggagatat ggtcaacatg gataccggaa gtgatcgtac aacttcccgg aacggcagtt
      901 ttgatgatca cagctatttt ttcggctaag acatcgtctt tatggaaccg accatcgtgg
      961 ctagggaaac gtacagtttg catctctgta tcacgagaaa atgtagtgac tgttccgata
     1021 tgtggtgtat ctccgcctgt taaagtgatc aataagtctt taccgattac tttaacagca
     1081 gcttggatcg tgaaacctag tttggttact tcaactatcg tttctttttc catattaatc
     1141 accctgccaa cgttttgaat gctcatttac aatttcaaat tgatccaaaa gtttcattgt
     1201 atggtgatta acaagatctt ggatcgtttg aggctgatta taaaaagcag gaattggtgg
     1261 tatgatttgc acacctagtc gtgctaattt agtcaagttt tccaagtgaa tggcatttaa
     1321 tggcgtttca cgaggaacaa tgactaactt tcgttgctcc ttcattgtta catcagcagc
     1381 acgtgcaatc aggttgtcgc ctaaaccaac agagatacct gcgactgttt tcatacttgc
     1441 cggtacgatg atcattccat cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc
     1501 tttgtttgaa taatggtaat caaacaggct ttcgaattca gttagtgata agtcggattc
     1561 cagctttaag ttctgttttg cccatggact gatgattccg tgtgtttcta tcatcggata
     1621 ttcttttagt ttcttcacta ggttgatggc ataaatggtg cctgaagcgc cacttacacc
     1681 aatcacgatt tttttcttcc gttgtgccaa ttgttccatt ttccgcactc ctttttatga
     1741 tgtcaaaaca gttgcagatc attcattatt tcaaatattt ttcccaatct tctacttctt
     1801 ggaattttgc acgtacaaaa tgttctttca tatcgaaagg aactgtaccg tcaaagatag
     1861 tttttgcact tatcccacgg aaacgaatag agccaggatt atattctggt aattctgatg
     1921 ggtccaatgg atggttcctc attcctggga tcacgatgat atctttatct ccttggaaac
     1981 gagtgttgaa agtccaaact acatcgttca tgtcgaaaat atcaacatct tcatcgacta
     2041 aaatgactgt cttcaattct ttgaaggctg ataaagcaag aatagctgct tgacgctgaa
     2101 taccttcatc cgcttcgttt tctttacgga tttgcatgat ggtcatcaat ttaccaccac
     2161 ctgcaggagg attgtagaca tttactactt ttcctggaat agctttatcg accaaggcaa
     2221 gaatacttgc ttctgtagga ataccggcca tagatacgtg ttcttcactg ggaccaatcg
     2281 ttgtttgcat gattggattg tcttttcggt gagtaactgc tttgactttc actacttgta
     2341 cagctggatt agcatcccca gtatagccag gaaattctgg cattgctttt cctgagttcg
     2401 tgttgatatc ttcttgcatt gtttcatttg gcatgatttc tgcttcaatg atgaattcag
     2461 cacgagcaat acccacttca tcgactgcta taccatcaac gatttgaata ggttcgttac
     2521 gcaaagctcc tgcacaccat agttcattgt agccaagtgg agtggtaggt ggttcgaaag
     2581 tcgtgccaat ggcaattgct ggatctaaac caatattgat agtgataggc attggtttat
     2641 tcaaggcttc gaattgtttt tggaagtgac caatgtggcg tccgccaggc atgatgtaca
     2701 taccgatcgt atctttatct tctagaacca tacgatggat cgttacatcg ctcattgttt
     2761 tttctggatc tgatcctaag actacaccca ttgtgatgta tggaccagca tcttcaggag
     2821 tatttgtagg tgcaggcaaa atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa
     2881 cgacttcttg tgctggagcg tctgctttgt ctactttcac aggtttgatt ggattttcta
     2941 cagcgtcttt caagaaacga ccaagtgttc gataatcatg gtgcaaaatt tcacctacac
     3001 gtttacgact agccattgtt ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt
     3061 tgaaagtcat agttggtcct tcttgagtcg gacgttttac tgtaccgcca gcaccgatat
     3121 aacggtaaac accagataat tctgcttcag gatcgatttc aatatctgtt tcgtgatatt
     3181 gacctggaag tgtttttagt tcttcgatga cttttcttaa atcatacggt tgatctgtca
     3241 tgagagattc ctccttagtt gtctttacct atctatagta aaaggtgaaa aagaggaaaa
     3301 caattcaact tgttcggtaa agtattctac aatggaatgg atgcgttttt ttgataatag
     3361 aataaactat gatatactta tcactaaaag aaagcgcata cttagtgttg gaggaaggaa
     3421 agtgaaaaaa atggaggagt catttcctaa agcagtaaaa gtggaaaata tagcgaatat
     3481 tttgaaagtt acatttgaaa acggagaagt aaaatacgtg aaatctcatt ggacagaaga
     3541 gataaccgat gccttgcaat ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc
     3601 actttctcgt aatatgtgga ttgggactga agtaacgatt gaagcagatg gaacagtatt
     3661 cataaatgga aaagacaggt atacgccaga agagttgtgg tacaaaggaa agaaaagtat
     3721 accagaatta taaaaaataa ttaaaaaaaa cagcgcgaaa caaggaacat attacttcct
     3781 cgtttcttgc tgttttctac tatttcagcg ctcttatgac tcaaatctaa atgtggttgt
     3841 cacaacctta ttctgcacgc aaagcgatag cggcgtcttt tttcgctgcc attcgtgcag
     3901 gaagatgacc gccaagcatc gtcaatatag tagagacgat cactagaata atcgcatgga
     3961 caggatttag ttgagcaaca ttttttagat ccgtcatgtt gtataagatg ctattgatag
     4021 ggaacgtagc tagccatgca ataaacacac ctaatatccc agaagaaata cctaaaatac
     4081 acgtttctgc gtcaaatacg cgagtgatgt cttttttacg tgcacctaaa gcttttagta
     4141 caccgatttc ttttgtccgt tcaatcacag atgtataagt aatgatactg atcatgatca
     4201 tacttgttac taaagagatc cctgcaaaag caataaggac ataagtgata gcatccatca
     4261 atccgccagt cagttcagtc atcgttccag ctaaatcagt ataaatgatt ttatcttcat
     4321 ctgatttgcc tttattgtaa tcatctagat aatccaagat cttttctttg tcttcaaaat
     4381 tgttcggata aatcatgata cttgaaggca agctgtctcc acctaaataa ctaagcaatg
     4441 tttgtttggc attttcgtca actttttcag tagttaagac atttacacca ctgtctcttt
     4501 gagctttgac gatttctgat tctttgtttt cgttgacgat ttgagtcgtt aattgatcgc
     4561 tataagcgat tcctggagat aagagattca ttgtagaaga agatttcacg cgtaggatcc
     4621 ccgagatctt caattcatca gaagcattct gatacatcgc atcataatct gtattaggga
     4681 taaaattccc agttggaagt tttgtatagt aagtattgtt gtttactaat ttgaatgttg
     4741 taccaacgat atcatcaaaa tcaagttttt cgtcttcttt cacatcaaat ccaagattct
     4801 tcaatgcgtt gatattagta ttattattgc cgtctacgat cagcacgaca tctgttgctg
     4861 aggctggata acttccggca agtaaagaat aattgtcttt taaaaagttt tctttgctag
     4921 tgtcaagctg tgtagggaaa cttgacactc ccacacctgt cattgctgac atcgtactag
     4981 ataaagaaag agattccgta tcaggatttt gattcgagaa gctgacaggc tgtactttgc
     5041 catttacatc acgtaaaaga ttgattcctg tagtacgggt aaatccgata ttattactta
     5101 agttaggatc gatgtctgtt acgtaatcga cgtatgtttg atcaatctta ttcgtgtgct
     5161 gtgctttatc ttcttcgctg acttttgcag taatcgtttt gctgtctgga taatccgctt
     5221 tgcttgcacc caatccggca tcgtcacgag tctggttagt cgttacctta gaaatagtaa
     5281 tcggaaattt cgccatcgtt tcagcttgcg tgttatcgat ttgtttttgg aatccagaag
     5341 aaagggacag aacgatcgcg attccgatga tcccaatact ggaagcaaag gaggtcaaga
     5401 aagtacgtcc tttttttgtt cggatattgt tgaaggaaag ctttaacgct gtccaaaaac
     5461 tcatctttgt tctgcgtaag ttaaattgat catcttttgg tcgttcgatg tgaggatggg
     5521 aatctgttaa gatcttacca tcagaaaatt caatgattcg atctgcgtat tgatgggcca
     5581 gttcaggatt atgtgtgacc atgatcacta atttttcttt cgataactct tggatcaatt
     5641 ccatgatctg gatacttgtt tctgtatcta acgctccggt tggttcatcg cacaaaagga
     5701 tatctgggtc attggctagt gctcgagcga tggccactcg ctgcatttgt ccaccagata
     5761 actgatttgg ctttttatgc atatgatttg tcaagccgac tcggtgcaat gcatcttctg
     5821 cccgttttcg tttttcgtct ttagaaacac cacttaatgt catcccaagt tcaacgtttt
     5881 caataatgcc tagatgacca atcaagttgt aactttgaaa aatgaaaccg atagagttgt
     5941 ttcggtaggc atcccagtca ctgtccttaa agtctttagt agactttccg ttgattacca
     6001 tatcaccgga atcataatta tctagtccac caatcacatt cagcatagtc gttttaccag
     6061 aaccactagg acctaaaatc gctacgaatt ctttttgtct gaaagcgaca gacacaccat
     6121 ccaatgcttt ggtggtggtt tcgccgactt tataatattt ttttatatct tttaattgaa
     6181 gcataatcac ccttctcccg tattgaaata ttcggtatac taataagtat atcagaacaa
     6241 tgatcgtagt atacgaaata aatgttaact gaagatgaac tacaagaaaa aatctagaaa
     6301 tttttcaaaa aacagaggga gatcaatgaa tcgtatttta gtagtagaag atgatccgca
     6361 attacagttg ctttaccgtt ctgtgttgga acgagcgggc ttttcagtca tcaccgcatt
     6421 gaatggagag gatgcgctga aaaaactaga agcgacacgt atagaattaa tcattacaga
     6481 tatcatgatg ccgaaaatgg atggctatga cttgctagac tcattacgta gcaccagatt
     6541 tgacacacct gttctaatga ttacggcaaa ggccgatttt gaggataaga aacgcgggtt
     6601 caaattagga gcagatgatt atatgacaaa acccatcgat gtcaatgaga tgatcctacg
     6661 tgtggaagca ttgcttcgac gttcaaagat caattacagc agtagcttga taatcggaca
     6721 aacaaaatta gagctagatg cttatacagt gttacaagat cagcattcgt tgattctgcc
     6781 tcaaaaagaa tttctgcttc tgtataaatt actgtcttat cccaacaaga tcttcactcg
     6841 ccaacagttg atggatgata tttggggatt agatacgaat acagaagaac gaacgatcga
     6901 tgtacatatc aaacgtttaa ggacccgttt tgaaaagacg gaagattttc aaatcatcac
     6961 agtacgggga ctaggctata aggcggttat ttaaagatga aaaaaaagct attttcacaa
     7021 ttatggatct attttgctag tattgtcttc atttcgatct tagtgacact gctgtgtttt
     7081 ctcggtttta ttttcttatt gtctcgacat tctatctcgc ctgctgaatc ggctggaaaa
     7141 ccaacgttat acccgttatt cgtctttgcg gggttcagta tggtcgtggg tacaggaata
     7201 tcgatttttg ttgggcggcg tatcctgcat ccaatcagtg cgctagggac aaatatgagt
     7261 ctggtagcaa cgggagattt ttcgattcga atggatgaac agcagaaagt agcagaagta
     7321 cagcaactgt ataaagattt caacgttatg gtccaagaac tcaatagtat cgagacactg
     7381 aggaatgact ttgtgtccag tgtatcccat gaattcaaaa caccactagc aacaatccaa
     7441 ggatatgttc aattgttaca agccccaaat ctctctgacg aagaacgcca aatattttta
     7501 tatcggatta tcgaaagtat cacgcagctt tcacaattaa cagagaacat cttaaaattg
     7561 aacaaactgg aaaaccagcg tatccaactg gaaaagaaag agtatcgatt agatgaacaa
     7621 atcagagaag taatcgtttt ttttcagcct aaatgggaga aagaacagct agaattggat
     7681 atcgaacttg ctgcagtaaa ttacacaggc aatgaagagt ttttgtacca agtgtggctg
     7741 aatatcatgg ataacgccat aaagtacaat caaatcaatg gacagattca tatcaaacta
     7801 tttgagacag ccacagagat tgtactggaa gtgaccgatt caggtgtagg aatgaatgaa
     7861 gagaccagag atcgaatgtt tgaaaaattt tatcaaggag atacaagtcg tcaaatttct
     7921 gggaatgggc taggactttc actggttaaa aaaattctag aactgcatga tggacgtatt
     7981 gattatagca gtatcgaagg agtaggaacg acagcaatga tacggttaag taaacaatag
     8041 gaaaaagaat aaaaccccta aaaattctat tgaaggtttt taggggtttt tagtcgtttt
     8101 tttgtgatta gggaaatgtt cttacggttc ttttggcata aaaatcgctg ctttcttatt
     8161 gaactacgag aaatgttaga ggtgtcattg aaacaaggac atgatgcaag aaagagagcg
     8221 aaagtcatgc gtcgctctct ttttttgggt ggatagatgg acagaaaatg tagggcttag
     8281 ttttcttctt gatttaaata ctcccaaaat gtatctgaag ccaaagaatg ctcaccgact
     8341 ttacgagtaa atgcaagata actctctgtt gttggagaaa tagggagaat cacagtattt
     8401 tcttttaata gattcttgac tgttttttcc ataacaatag caatccccat gtcatttgca
     8461 atcatattga tgatcaagtc gattcttgct cctttataag agatttttgg ttcaaatcca
     8521 gcttcgcgac acagatctag tacaggttga tacaagttcg tagtggggcc aaggagcaaa
     8581 aagttctcct ttttcagttc gcttagatcc aatttctttt tactagctaa aggatgattt
     8641 ttaggtagaa ctgctacaaa atcgtctgtt tcagtaggca accactcaat cgaacttggt
     8701 ggggcctgga aacttctagc aaagtggatc gtgtcactat gttcattggc gtcattgtaa
     8761 gttaattcga cactttccac ttctttcaac tggactgtaa attcaggatg gcgtttcaaa
     8821 aactcggtga ttttggaaaa acctttataa ttgatcattg tcggtatcgt taagatattt
     8881 aatgaaagat tgttgtcttt cgtataggtt tcaagcactt cttgcatagc atggtattgt
     8941 tctacgacat tttttacata tgggagcaat aagcttccag cttcggtcaa ttcgattttt
     9001 ctattggcgc gttcgaataa tttgacgtct aattctgttt ctaacgaaag aatctgttta
     9061 gaaatatttc cttgggtggt gaattgtcga tcagcagttt tggtgaaatt cattgtttcg
     9121 gctaaatcaa gaaaaacttc gaatttatgg atatccatca tattcctcct acttattcca
     9181 ataaagaata ctttaacaca aaaaatgaat tggtgtaatc ttctctgtct gttacaatct
     9241 ttcttgcagc gaaagcaata attacagaaa tttttctgat ttttttcgct tcgtagaaaa
     9301 agagaggagt tttttgatgc caactggagt tttgatcaat gtaggttccg ttttgtttgg
     9361 cggactaatt ggcggattag taggaaataa attaagtgaa cattttaaag cacagctgac
     9421 gatggttttt ggtgtgtgtt cgatggggat gggaatctat tccattgcgc cgatgaaaaa
     9481 tatgccggct gttatttttg ctttagtgat aggaacagcg attggtttga ttgttcattt
     9541 aggtaatggg ataaataagg gggctgcgct gatgcaagta cctatttcta aaatcttccc
     9601 tagtgaaaag cttggtatga cccacgatga atttatcagc acattagtga cggtcattgt
     9661 tctattttgt gcaagtggta cggggatcta tggaagttta gattctggga tgacaggaga
     9721 cagtacgatt ttgatttcta aatctgtcct tgattttttc acagcagcta tttttgcgtg
     9781 taatttaggg tatgtcgttt ctgtgatagc aattccccaa ttcattattt tttatatttt
     9841 gttcttattg gcaaaattta tttatccatt gactacacca gacatgatct tagattttaa
     9901 agcttgcggc ggattcttga tggtcgctac cggttttaga atgatcaacg tgaaaatgtt
     9961 cccagtagct gacatgatac ctgcaatgat cgttatcatg ccactgagct ggatgtggac
    10021 aaattggatt atgccactgt tataataaaa atcaaagaga agccattttt tgctgactaa
    10081 aaaggatcaa atcttttagt cagttttttt gtatttgaca aactgatttt gaaactatat
    10141 tatatgttgt ataatataga ttgaaataaa atattacact tgagaataga aggtgacgta
    10201 atgcacatcc agattccaac tgttcttctt gatggtactg tgctagcagc attaagcaag
    10261 gaagatatgt atgggtatgc cttaacaaaa atcgtacaag aaagtctctc tgtcagtgaa
    10321 tctacactat atccggtttt gagaagattg aaaaaagaag gatatcttga gacctatgat
    10381 gaaccttttg aaggcagaaa ccgtcgatat tactcgttga caccaatggg aagtcgtcat
    10441 ttagaagaaa ttattgatga atggattgtt tttcgagatt ctttagaccg gttattggag
    10501 gagagcacaa atgaatgaat atttgaatga agttgtggat catcttcaag aaattcctga
    10561 agacgatcgt tttgatttga tccagtatta tgaggaatat tttttagatt ctggaaaaac
    10621 attagatcag atcattgatg aatatggtac accaaagcag tttgccttaa aattaaaaat
    10681 cagttacttt tcagaagaag aacaagcaga aaacgagtta gtgaaaaatc caaaaagaca
    10741 aattcgcctg atttggttaa tcattatcgg tttatttgct tctcctgtcc tcattccact
    10801 agcactgggg tttatattgg ttattggggg actattggtt gctttactag cgctcatttt
    10861 tagtatctat gttttatgta tcagtatctt aggtgttggc ctattcatga tcatctcagg
    10921 aatcagtgtg tttggacaat cgattctgag tggattattc tttatcggtg tcggtctgtt
    10981 cgccacagga agcgcagtat ttatttcgcc actgttgctg aaaggaacaa aagggctttt
    11041 ttatgtcatg atgaactttg ttaaatggat gggacgacgt tttattacaa aaagagatct
    11101 gcattcagcg ggcgtttgaa ggaggacaca taaattgaga ttgaaacatt ttttgattac
    11161 cggtattggt ctgatgctta ctggtggcct cctagcaggt gcaacttata cattaggcgc
    11221 tcaaaagagt ctgacttggg ataatggtcc tcggttgatc gatatggtta ctgagacaaa
    11281 aaaaatcaag gatgcaaaca aaatcgtcat ccatgcagaa aatcaaacag tttcggtcat
    11341 acgcggattg gattttgaga taaaaacgac ttatgataag acagaaaagc cagaaataaa
    11401 ggagaaaaat ggcgtgttga ctgtgaatgc agtcaaaaaa tcaacaggtg caatcattag
    11461 tttagaagac acaagtgcac aaatcactat tgttgtccct gcagatcatg aattatctga
    11521 attgacaatc gaagggaata atagtgcaat tgatatcaac gaagtatcaa tcaaaaaagt
    11581 gaatactaag acacatgatg gctgggtatc gttcaatgaa tttacaggag gaacaataac
    11641 tgcgaaagat gaattaggct cacttcgatt agatgaggcc aatgtggata acttgatcgt
    11701 cacaggtgaa aacagcaact tccatctaga agacagtttg atcagcaaag aaggaaaagt
    11761 catcctaaac ggtggcaatt tatctttgca tgagatggaa aacagtggct atactatcca
    11821 aacgtctggt aaatcttata ttgaaaaaga ctaccagcca ataacaaata ctttgttaga
    11881 acaaggacag aaaaagctaa agatcactgc taaagacaca tcgatcaata ttacgacgga
    11941 tcaagaggac aatgaaacag attgatcctg tactcaactg gatattttaa gaaaaaacag
    12001 caaagatcct tccaatcttt gctgtttttc atcgcttaac gcacataact atagtaaaat
    12061 aagaaagaaa atatctaaaa agaaaacagg gtgaaagagt atatgaaggt agaagaaggt
    12121 caaagagaaa agttaaaaac agaaatggaa agattacata cttatatcac tcaactaagc
    12181 caaaccttct atgatccaga caaagaaaaa gtgatggtta attacccgaa caattcagaa
    12241 ggtcgccaat tagagcaagt gtaccatgaa gttttcaaac atttattgac ggtgaagaaa
    12301 gagctggatt attattcttt accgattatc gatacaggaa tattgaagta tgatgggaag
    12361 aaggaacgat ttatctttaa atctgttcgg gaagatttgc ctctttcagc agggatggat
    12421 ctagaggtct tggtagaaga ctattttaca gaagaaaagc actgggttcg aacaaaattg
    12481 gattatttac cgcaggcagc cggagggaca caggcaagcg gttggtacat cactgaagac
    12541 aaagagttag aactagaagg tgtaatggcg cgtattcgaa aaaaaaatta aaaaagtaac
    12601 attagatatt tattgaagga taaaggagcg caggaagtag catgagagaa aaaagagcat
    12661 tttatatcaa tggttatgca gggattgtcg gattggtcgt attagcttta gttggattgt
    12721 ttttcttcta tttaggtatg tggcaagcga aagtttgggc acttttttta agtatttttc
    12781 tttggttgat cacgttattg ttgttaagtt cggcaactgt agtcagtcct aatcaagcaa
    12841 aagtaatttt gttttttgga caatatttag gtacgattcg agaaaatggc tttttcctta
    12901 cgataccttt ggcacaaaaa atgactgttt cgttgaaagt tcggaatttc aatagttctg
    12961 ttttaaaagt caatgatttg gatggaaacc ccatcgaaat ctcagcagta gtagtcttta
    13021 aagtcgttga tacagcaaag gcattattcg atgtagcgta ttatcaagat tttgtagaaa
    13081 tccaaagtga gacagccatt cggcatatcg ctagccaata cccgtatgat acattcaatg
    13141 agaatgattt gacgcttcga ggaaacacga cggcagtgtc tgacgagttg caaaaagagc
    13201 tgcaagagcg tttagcagta gcaggtgtcg aagtaatcga aacacgcttg aaccatttag
    13261 cctatgcaac agaaatcgct agtgccatgc tccaacgtca acaagcaaaa gccattttgt
    13321 ctgctcgaca aaccattgtt gaaggagctg tcacaatcac tcaaatggcc ttagagcaaa
    13381 tcgaagatgg acaagaaatc aatttcaccg atgatcggaa agtccaattg atcaataatt
    13441 tacttgtctc aatcattaca gacaaaggaa cgcaacctgt tatcaataca ggcgacatga
    13501 aagaccgtaa gtgacatact ttttgtaaga taggaagaag gagtttagga cggattattg
    13561 tcttgaactc tttttttagt tgttttaaag aaaatgacaa aattgaaaga agaatgatag
    13621 ttgattcgaa ggcagaattg ttcgcttttt tctataatca gtatataaag taagagagaa
    13681 ggggaaaaag tgaaaaaact tctgatcggt ctacttgttt tcatcgttgt ggtacttgca
    13741 ggggtgaaaa tcgcagatta cgtagttatg ggtggagaag aatactatgt acaaattacg
    13801 acagatggta caaaaacagt ttcaaaatca gactctggac aagaaacagt caactataga
    13861 tatatcctgc ctggctatac gaaagagggt aaggaaaaag aactggactt caagggacaa
    13921 ttgactcgac cgcttcgtaa aggagcatat ttaaaaatca cctggaacaa aaataaaggt
    13981 gtcacttcgt atgaagaagt cgaaaaaaaa gacgtgccga aaacagcact ggaaaaacta
    14041 attaaagggt agggaaagaa atgaacaaaa tagtcgaagt acagaatgta acaaaagtat
    14101 acggaaaaaa caatgagaaa aagacgcaag cattaagcgg catcagcttc gatgtagaaa
    14161 aaggcgaatt tattgggatc atgggcgctt ctggttctgg taagtcgaca cttttgaata
    14221 ttttatccac attagataaa ccaacagatg ggcatatccg aatcaatcaa aaagatgtga
    14281 ccacattaaa aggaaatcaa ctagcagatt ttcgtgcgaa tgagattggc tttattttcc
    14341 aagatttcaa tttattagaa agcttgacag cacaagaaaa tatcgctgtt cctctttctt
    14401 tacaaggcgt cagaccaaaa gaaatcaagc aacgtgtcca aaaaattgca gaacgtttat
    14461 ccatctctca tattttggaa agctatcctt ctgagatttc cggtggacaa aaacaacggg
    14521 tcgccgccgc acgtgcattg atcacacaac caacgattct attaggggat gagcctacag
    14581 gtgcgctgga ttccaaaagt gcccgggatc ttttagatac gatggacgaa ttgaatacaa
    14641 aagatcatgt atcgattctt cttgtgacgc atgatccgtt ttctgcaagt tattgccaac
    14701 gtattttatt catcaaggat ggcggtatcc atcaagaagt gaaacgtgga gatcaaagcc
    14761 gtgattcctt ctatcgagaa atactatcta ttcttggaaa cttggaacag tagggggaga
    14821 ttgactatgc tttggaaatt atcacttact gggatcaaga gccgtttaag agactacttg
    14881 gtgttattct caggattagt tatggcatct gctatttttt acatgtttga atcaatggca
    14941 agcaacgaag cttttttgaa aagtaatacg atcgttgcgg ctacagttat cattttccga
    15001 ttcggttctg ttttactagg gatcatcaca ttcgtttata ttttatatgc aaattccttt
    15061 ttgatgacga tgcggcaaaa agactatgca atgtttatga tgctgggagc aaaaggcaga
    15121 aaaatcgcac agatgatttt ttctgaaaca ttcttagtag ggatcgcagc tactttagtg
    15181 ggttcagctg tcggtgtagg attgacaagt atcgtccacc gattattagt ggatcagtta
    15241 gatctcaata tttcgcattt tacacctttt agtattcacg ggttgttgat cactgttttg
    15301 ttttttgctg ttttgttttt attagctgct gttgtcaatg ctttttcgat tgtcaaaaaa
    15361 ccgattttag cattgattcg tgcggatcag acaccaacaa gaatcaagca gcataaattc
    15421 ttgtttttcc ttgaagtagt actgggaatt gcttttctag gctgcggtta ctatatgatg
    15481 aaacacgtgg ggacctatca agtctttggt ataggggtag ccttagttac gatcgtatta
    15541 ggaacttatt tcattttcca ttctgtgatc attttctttt tatcattact aaagaaatca
    15601 gaacagattt cattgaaaaa attgaacaat ttcacattgt cacagttaaa ctttcgtatt
    15661 cgtgaatata cacaaatgct ctcaatggtc gctatgctgt ttgccttggc actgggcgct
    15721 ttgacggtag gattaggttt taaaaatgaa attgcagact tgacagaaaa caattctgga
    15781 tatgatctag tgatgaataa tcctcgggca gaagatcagc aaaaaatcaa agaactttca
    15841 ccaacacttt ctgcttctta tactcaaaaa gaagatgcgg gaacgattta ctataatgca
    15901 agtgaatttg cggctcaacc attgatcatg ataaaacatg aagatggaac accgccgaag
    15961 atcacgaaag aaaaagttcc tgctaaaaaa atgaatgaac tgactgtaca agaggaactt
    16021 cgaagctatg aattgccgga acagcaagaa aaagaaatca aggtagtcag tcaatcagaa
    16081 tttgatcaac tgaacttgcc acaatccact ttacagctag ttcaggtaaa agacttccaa
    16141 gcaaacctga aaccaatcga aacacttgtt gctcaaaata aaatgaacaa tccatctttg
    16201 caaaaagtag agtacagcaa ccagaaatat gaaatgtatg aagtgtataa tggtctattc
    16261 tctggttttg aatttatggg attcttccta ggtttagctt tcttgaccat gcttgctagc
    16321 tgtttgatgt tcaagatctt gtcaggaagc aagagtgaca ttgctcgtta tgaaatgctt
    16381 gaaaagatag gaacacgacg cgggttgctt aaacaatcga tccgtagaga agtaggcgtg
    16441 ttattcttag caccaggaat cttaggtgta gtccatgtct tattcggatt acaaatgttt
    16501 ggtctattca tgcaaaatcc atacaaagat atttgggttc cgtttacgat attcttcgta
    16561 ttgtatttca tctactatgt actgacgaca tggttatata ctggaatcgt attgaaaaag
    16621 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa aatagctcct
    16681 catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt
    16741 cggaaaaaga ggttgtgaca atatttttgt cacaaccttt ttttcacggt cttttattag
    16801 aatatagtaa attttttctc gttactcacg gaataagctg ttaagaaaac ggtttccatg
    16861 tatgctggaa tcagtgcaaa tgaaagaaaa gagggtgcaa ggaacatgga aatcaatgaa
    16921 caaaattttg gcgaaaacta tcacttgatt acgatcacga atcaaaatgg gacatctttg
    16981 tctgtcagtg atcttggggc acgggttgtt cattttcaaa caataatcga taatgaaaaa
    17041 cgagagttga tcttaggttt tgatgaagca aaagaatatc tagaaaaaga cccttatatc
    17101 ggtgcttcaa tcggacgtat ggctggacgg attacgaatg gcacattcaa attagatgat
    17161 gaaacgttcg aagtaaaaac agacccggac actggtcatt ggtttacatg gtggtgcacc
    17221 tagctttgaa gccaagcagt ggcattatac agtcatcaat ggtgaaaacg aagcatcggt
    17281 cattttctat cagactagcc cagatatgga aaacggattt cctggaactt tagatgtaga
    17341 agtacggtat acgctgacga acgatgatac atggcgagtg acggtccaag ggatcagtga
    17401 ccgcaaaaca gtattcaatc ctacaaatca tgtctatttc aatttaaccg gtgatccagc
    17461 acagtcaatc gacgaacata tactttggtt gaatagccga ttatttgcta cgctcaataa
    17521 agatacaacg cctacaggag aagtagtttc agtaagtggc acagcatttg acttccaacg
    17581 tcctaaaaaa ttaggtgagg tctttgccag tgattttcgc caatcaatga tagtgggcgg
    17641 catcgatcat ccatttttgt tgaaaaaaca atctggttta ctaaatgctg cggaattgac
    17701 aagcccagat gaaaaagtat cgatccaagt aaaaacagat gcttcaggaa tcgtgatttt
    17761 tacagcaaat tttggagaag atggaccgga aatgcgcggg aataaattag taaatcatgg
    17821 cggtatcaca tttgagactc aagaactgcc aggagcggag cgacttgaaa gttttggaga
    17881 tgtcactctg gaacctaacc aagtacgcga gtttgtaacg gaatacaaaa taaatataaa
    17941 gaaatagaat aaattaaaaa gggagtgtga cagtgtcaca tccccttttt aatttattct
    18001 atttctttaa aggcagatca agccagaatg atttgtctaa acttaacaag gttagtacag
    18061 gaacagttgc agctttaact gtacgagtag ttgtttctgg tatctgtctt gccgtaaaat
    18121 tgttccacaa atcccagatt gaagcgacac ggaagatgga agaagcgttg ttgacgcgtt
    18181 ttatcgttga aaattctaat cctgtgattg gcacgccatc tgttgtccat ttgattggca
    18241 catcgaatcg cttagacata ttgaggattt cagcgtttgc ttcttcagtt gttgctgctt
    18301 cttttttcgg ttcatcatat tttgtcaggt cgtacgcttc gatgatcgcg ttcaattttt
    18361 gtgaatattg gcgatctgta gcataacgtc cttccaaata tttggttgct tctttgtagt
    18421 cttttgtttc cgacttccaa gttggtttgt agaaatcagg attgccagtc agtccatttt
    18481 tgatcaagtc ggcatagtct tccaaacttt ccttgtaaga aggatattta cggaattgtg
    18541 cgcggatttg gtagctttgt ccggaactat cttgttcaag tgtattgaag gtcacgctgc
    18601 ttcctttata tgatcctttg ataccaaaaa ggttatgatt tggttcgctt gataataagc
    18661 tgtttccaga tccactttca aggatagctt gagccatcat gactgatgcg tagattcctt
    18721 ctttttgccc gatttcctgt gcatcatctg caatcttttt caagaactga tcagttgttt
    18781 ggttttgact gaaatggaac gagccatcta atccatctaa aatagatgag ccgcctgtca
    18841 gcgaacctga agcagcaggt aaacctgcta agtttactcg gacagcaaaa tcaggcgtga
    18901 aatctgaaat agaagttact cgaactttat ctcctggttg aggcgcatgg ataaattgtc
    18961 cgttcccaat atagatacct acatggtgag taacaccgga agaaggtccc cagaagacga
    19021 ggtcgccagg ttgggcctga cttactggaa tctgcgtacc tgcatattgc tgttcgcctg
    19081 tccagccacc gatataatga ccggttgctt tcatatatac gtaataagtc aaaccagaac
    19141 agtcaaaagt atttggacct tttgctcccc atacatatgg ttttccaaga tgcttgaatg
    19201 cttctcgaac gattgcttgt tggacattcg ttgtatttgt aggcaataca gcatcaattg
    19261 gtgattttac aacaaaatca gaagaagcag aagatggatt ctctataccg ctttgatttg
    19321 ctgtatttgg acgagatgaa gcattgttcg ttccgccatt cgatgaacca ttgtttggat
    19381 ggtttggttc tctctgagta tttccattgt tattcgatcc agatgggttt gtttgattcg
    19441 aagaattccc gctgttcgaa ttattgttat tattgtttga attatttgaa ccattattat
    19501 tgttgttatt acccacatct ggtttgtttg gcgtttccgg atctggtgta ctagggactt
    19561 cttcactagg atcagttggt ttctctgttg aagtatccgg tccagcttta aattctgcag
    19621 taatagataa attgttcgta cctactgtaa aggtctttgc atcataattt acttctttgg
    19681 caccattaac agcgactgaa gcaatttgtt caccttcagc tggcgtaata gtgtaagtta
    19741 ccttcgtttc cgctaataaa ccttctaccg ttccactttc cgtaatatcc aaaggttgct
    19801 gttcagggga atccgctgtc gaagcagtca gttggatctt accaatcaaa gaaggagcaa
    19861 ttgtaaggga atatgtctcg gctggctttt cattttctga tggatcagcc ggatcaattt
    19921 cagttggctc ggtcgatcct gttgttgagt tagttgttcc agttgggtct tctgtaaaag
    19981 aatccgttgt ttcactagta gctgagctgg atgtagtcgt atctgttgtg actgtttgat
    20041 cagctatttg attatctacg gcaaatacgg tattggtcga caaaacagac ggagcaatca
    20101 gccaagtagc agctagcaaa cgtatcgttt ttttattttt cataataaac tagtcctttt
    20161 cctttcattg attttctgtc ttattcagca ttcattagac attttttgga acagataatc
    20221 gtgtcgaaaa gctttttctt ttcataaatc tgtctgtgta ttctaaataa aacaatttgt
    20281 aaataagcaa cgagcattca ttcgtttttc tttgacgttc attttattgc atagaccacc
    20341 tttgtttggg gataaacaag tggggataaa aatggttatg catatgtaaa ttataccaaa
    20401 gtttggaaag tatcacaaaa atttataaaa ataatgatcc taaaaagaaa aagactgaaa
    20461 tatgtgataa acatctcata cttcagtctt tttcattaac tattcataag acattaggtc
    20521 ttcatcaaac ccgctaacga gagtagtgat cattgctacg actagactaa cagccagtcc
    20581 tgctaagtac cagaaaaaag taccggaaat gaatgcaggc aatgtggtca gactaccaaa
    20641 tacaaaagcg atcgctttca cttgggcaaa tcccattaca gctccaccag cagctgcacc
    20701 aatgcaagca gcaacaaatg gttttttcaa tgggatcgca attccgtaaa cagttggttc
    20761 ggtcactccc gcaagtaaaa tcgaagcaaa agcagaaccg gagatcgaac gggtttttga
    20821 acgtttactt cgcagaaata cacctaaagc tgcaccagcc attccaaaat tggctgctcc
    20881 tgtcaacgga acgattcggt cgaacccata agcagagata ttactgatca tgacagggtt
    20941 tacagcccat tggataccaa atattaccaa aatattccaa ccaccagcga tgattgcgcc
    21001 actgatcggt ccgttaatat ccatcatcca agtaattgcg gcagctaaac cttcgccaag
    21061 gtaaacaccc gcaggcccga ataccattaa cgtcaaagga accgtgataa ctaatgaaag
    21121 taaaggtaca aatactaatt gcatattttc gcgaatatat tttttgagga atttttcaac
    21181 aaaagaataa aagaaaatac ctaaaatcgc cggcatgaca gtagacgtat actgcatcag
    21241 tactacagga atccccagaa aatcagtagc tgctttgcca ctgtctgaaa gtaaagaggt
    21301 cagattcggt tcaatcagtg ctgcagcaat gacagccgca atatatccat tcactttaaa
    21361 cgttttggca gcagaaaaag caagaaagat tggcagaaaa taaaagacgg cattcgacgc
    21421 agcagacaaa atatgataag ttccgcttgt ttcactaagc cagccaatcg tagtcgctaa
    21481 aacaaccaga ccttttaata taccagaacc ggcaaacagc ggaatgatcg gcgtaaagat
    21541 ttccgatatc aaaccaaata gtttttcgat cggatttagc ttttctttcg gagctgaatg
    21601 atcgatttga cgcgtcaatt ccttgtaagc aggagccaat ccctgatcca tgattatttc
    21661 tgcatatcta tcgagtaaaa tgaaatactt aatggaaggg atttgctgtt ttaatttttc
    21721 tgcattggct actggctgtt ttttcaattt gatacgaagc cgagtcgcac atggaaggac
    21781 agaatcaata ttgtcttttc ctcccaactg ttcaatcagc atatgaatct gctcggacag
    21841 taccatctgt tcaggattca ttgtcgtttc cattagaaga ccactccact cactaatata
    21901 atattggcgt aaggtgtcat ttcgcctgta cgaataaatg catgacagtt attcaactct
    21961 tttttcattt cttcatgtgg gataaagcta atcggtgtat ctggtaacaa taatttgatt
    22021 ttttccaata tttccgggtt cattgtcttt atttcttccg ccaattcaat cgattgtacc
    22081 tcaagttctt ctaaaacgtt ttctaaaaca gataaaaaac ttggaagacc gtttgagaca
    22141 gccaaatcta ttttttcagt accaaagggg acaggcatgc ctgcatcccc gatacttaat
    22201 ttatcaaagt gccccatttg tgcgattact cgtgaaatat ctgaattgat tacttttcct
    22261 tttttcatgt ttgtttcttc ctctcattgt taattttaga ctgctgattg ttctaaagct
    22321 tcgcggtaag ggatagaagg ttgcgcacca taagactgca cagcaacaga agaggctaga
    22381 tttccataaa ggatcgcttc tttcaagtta ctaaggtctg gctgaagtgt gctgcttaat
    22441 gcgccaataa atgtatcacc tgcggctgtt gtgtcaacgg catccacttt aaaagcaggg
    22501 acgataccgt gttctttttc tgtatgatag aaagcgccgg cactgcctag tgtaatgatt
    22561 actgttccaa tacctaattc atggagtttt tcggctgctg cgaccaaact gttatgatct
    22621 gtcactctga ttcctgtaat gatttctgtt tctgtttcat ttggtataat gatatctgtc
    22681 ttttcaagta gttcagtagg aatcgtttca cgagctggtg caggattcag gatcgttgtt
    22741 tttcctgcgt cttttgcaat agaaaaagct aggatcgtcg catctaagct actttcaaac
    22801 tgagcaataa taaagtcact gtttgtaatc agttgcttgt tttttaggac ttcttgttca
    22861 tggaaagcat tatttgcccc tgcatggatc aagatactat tttctcctgc tgcgtctacc
    22921 ataatcattg cttgtccggt tgtggcgcct tggatttcag ctatcccaga tgtatcgatg
    22981 ttttcttttg tcagtagatc taataactgt tgtccttcgc tgtcagcccc aacgcctcca
    23041 ataaaagatg tccgtgcgcc tgaacgtttg gctgccactg cttggttggc acctttacct
    23101 ccgccagcgt gaaaaatctc atgcgcatgc atcgtttcgc ctggcttggg catgttggtt
    23161 aaacgaaggg tagtgtccat attaatactt ccaattactg taaccgtgtt cattttacaa
    23221 acctccaact atctcattta ttttgatggg aaaattattt tttaaaacgt tttagtaaaa
    23281 cgttttaata gttcgataaa aatatagcac accttgaaag ctatttcaag ggtgcagttg
    23341 aaaatctttt ttctaatcgt acaggtaatc ttttttcttc ccattctttt tctgggaatt
    23401 ggatccgatc taacaataac ttggcagatg tttgtcccaa ttcgaagatt ggttgtgcga
    23461 tcgttgtcag tttcggcttg atatactcgc acatatctat attatcatag ccaataatgc
    23521 tgtagtcttc agggatgctt tttccagctt cctctaatcc gcgatataat ccaaaagcta
    23581 attcatcatt taaagcaaac acaccagttg cagcagaatc aagaagttct gctgtgatct
    23641 ggtagccacc ttgttttgag aactgtgtag gtaagaggat cagttggtcg tgagggattt
    23701 ggtaaacatc aagagcagat ttaaacccct caatccgagc gtggacattt tccggtggat
    23761 tttcaggata gacaagggcg atcgtttgat gtcctagact aagcaagtgc atgcccgcta
    23821 ggtaaccacc gcgaaaatcg tctgttcgta cggcatcact aaatccttca gattttttct
    23881 gatccaaaac aatgaagggc cggccttgtt ttttcaaatt ttcgttgatt gcatctgtag
    23941 agacagcaga agtcgcgata atgaatccat ccacacctcg acgtgtgagc tcggctagat
    24001 attcgatttc tttttgatga tcagaatcag cattacataa tatcgtgacg aaattctgct
    24061 tatacaagat atcctcaatt cctcgcatca gtgtactgaa gaatggattc gtgatatcag
    24121 gaacgagtac accaattgtt ttcgtttcct tggtaatcat ttgtctagca aaatagtcag
    24181 gctgatagcc taattcttct ttggcagcta aaactttctg aacagttttc ggactgaatt
    24241 tctccgtatt tccatttaat atcaaggaga cagttgtgat tgaaacgccg gaaagtttag
    24301 cgacttcttt gattgtgatc ttttttttca ccatgttcat actcctatct aaatcctgtg
    24361 gttattttcc catgaaatca aaaagaagta aatgaaaatc acatgtgcca gtacttattt
    24421 gctttgttgg atagcttcga ttatttgttt cgttgagcgt actcgtgcag ttttcggaaa
    24481 tatctttttt atgaacaatt gatgggattc tttgtcacga tcactgcaag catcttcagc
    24541 aagcacgata tggtacccgt gttgaaaggc atctaatgca gtagcatata caccgtttgt
    24601 tgtcgctaca ccgcttaaaa tgatagtttc gattccacgc cggcgaagtt gaagatcaag
    24661 agaagtacca aaaaaagcac ttggattgta ttttgtgata gtcaacatat tatctgtatc
    24721 tttcaaagaa tccttcaaaa gtaaatcagt gaattctgga ggtacagaag cagtcattcc
    24781 acccatatca gtttgttgac tcaaataagg gaaagtggag atgtctacgt tcactaaaac
    24841 tttcaaggca gcggtacgtt tcaatgttct tactaacaaa tcattgtttg tcagaataga
    24901 tgctgcagtg tgtggagaga gtgtaccgtt atttacgatc tctttttgca tgtcgatcgc
    24961 gacaaaagca gtttttgata aatcaaaaat catattttcc tttaaatcat tcatgatgtt
    25021 ccttctttca aataacaatg ttttttatgt aagtaaatca taaatcgttt atttgtcaac
    25081 taaattaaag gattcctttc tttagtctag agaaattcag tggtaaaata aataagtgtg
    25141 agaaaacgca ttcgatgcat agatatagcg agaaatatcg tgaaaatgga ggaatcagag
    25201 tgaagtatgt cgaagtaaaa aatgaataca aacgatacaa aatgggagaa acgaccatta
    25261 cagcaaatga cgatatttct ttttcaattg aaaaaggcga attagtgatt atacttggtc
    25321 caagtggagc aggaaagtca acgattctta atattttagg tgggatggat acacctgatg
    25381 aaggtcagat cattattgat gacacagata ttgcgcaatt ttcagataaa cagcttactg
    25441 cttaccgacg aacagatgta gggttcgttt tccaattcta caatctcgtt cctaatctaa
    25501 cagcaaaaga aaatgtggaa ttggcaacgg aagtttctcc tgatgcgctt gatcctgtag
    25561 aggtattgag gcaagtgggc ctagctcatc gattgaataa ctttccgtcc caattatcag
    25621 gaggcgaaca gcagagggtt tcaattgctc gtgcactagc taaaaatccc aaacttcttt
    25681 tatgtgatga accgacaggc gcgctggatt ttgaaacggg caagcaagta ttgaaattat
    25741 tgcaaaacgc tagcagacaa cacggcaata ccgttttgat cattacacat aattctgctt
    25801 tagcgcccat cgctgataga gtcattcaca ttaatgatgc taaagtaagg tctgttgaat
    25861 taaatgatca tccttcatcc attgatgaga ttgtatggta gggggatttt atgaaaacaa
    25921 gaacatatct taaagcaggt tttcgcgaaa ttcggcaatc taaaggacga ttcattgcaa
    25981 tcatccttat tgtcatgcta ggaacacttt tatttgtagg tgtcaaaact gctggacctg
    26041 tcatgcaaaa aacgatggat cactatgttg gtacagcagg actttctgat ttgcaaatcg
    26101 tttctactgg tggcttgaca caaaaagata ttgctcaagc agaaaagatc ccagatgcaa
    26161 aagtagaaac aggaaaacag atctattatt ccaatactgg caaaaacgaa gtgattcaaa
    26221 tattttctta taacaaaaat aacaagcaga acaagctaca attaactgat ggaaaactcc
    26281 cggaaaaacc aaaccagctt gtattggatg aaaaagcgga agatgaaggg tataaaatcg
    26341 gtgatattta tcagattgat tcagatgaat tgaaagaaaa agagtatacc atcacgggct
    26401 ttgtccgttc tccattgttc atcaacaatt tggagagagg atacgcgaac gtcggaaatg
    26461 gtagcgtcga ttattttgtc tatttacctg aatccgtttt taaaacagat atttattctg
    26521 tcttatatct tgattttaca aacgtaaaga atttgaatac gtacagcaaa gcttacaaag
    26581 ataaaatgga gcaaaatcaa gaaaaggcag aaaagtattt acaagatcga cccgaagaac
    26641 gcttagaaga gctgaaaaaa tcggccaacg aagaattaga accggcacag caaaaaatag
    26701 cggatggcaa agcacaaaca acgcaagcaa gaacacggtt agaagatgct aaaaaacagt
    26761 tagcgcaaca gtcagcaact atccagcagc ttcctgaagc gcaacgaaca atcgctgttc
    26821 aaacattatc tcaacaagaa gctcaacttg ctgaacaaga aaggcaatta gttgagaaag
    26881 aacgtgaact ggcctctgca gaggaagaat tgaatcgaaa tgcagaaaaa attgaaaatt
    26941 taactaaacc atcataccta tacaatgaac gttcagaaaa tgtcggtttt caagaatttg
    27001 gtgatctgtc tgatcgtatc gctgcgattg ccaatgtttt tcctgttttc tttttcttta
    27061 ttgccgcatt gatcaccttt acgacaatga cacgtatggt agaagaaaac cgcagggaaa
    27121 tcgggacatt aaaggcgtta ggttatacga aagttgaaat cgcaaaaaaa tatgcaatct
    27181 atgcaagtct agcctcaggg ataggtattg ttttaggaac gatattagga acgaatcttt
    27241 tgccaaggat catttttgag ctgtctaacg agcggtatga tattgggagt gcgcttattt
    27301 tttatgactg gccgccgatt ttacaagcag ctgccgcatt cttcattgcc gcatttggcg
    27361 ctgcaatgat cgttttattc aaagatttaa gagaacggcc tgctgcattg cttcaaccga
    27421 aagcaccgaa gccagggaaa cgcatttttc ttgaatacat cactccgctg tggtcacgcc
    27481 ttagctttaa tcaaaaaatc agttaccgaa atctcttccg atataagtca agaatgctta
    27541 tggcgattat cggaattgct ggatgtgccg ggctgatggt tgcaggcgtg ggattgaaag
    27601 attctctaag ttctgtttca gccaaacaat ttggaacgat cattgactat caagcaatcg
    27661 ttacattgga ttcatccacg gaagatagtc gtcaaaaagc agcggatatg ctgcagaagc
    27721 aagaaaaagt aaatgagatc ctagctattc atactcaaac cgtagaattg agaaaaaaag
    27781 gacatgcttc gcaaagtctg acagtgatcg taccaagtaa aacagctgat ttggatcgtt
    27841 ttatccattt aactgatctt gaagagaacc cacgctcctt gcctaaaaca ggaatcgcta
    27901 ttacgcaaaa agtagcagaa ctatttgact tatctacagg agatcatatt tcgctttatg
    27961 atgaagatca aaaagaatgg aaagtcgaag taaaagaaat cgtgaaaaac tatctaggta
    28021 gctttattta tatgtcccct gaatattatg cttcagttac tgatcaatcg atgatggata
    28081 atgcattttt gctgcgtaca gataagatga caacaaaaga agaagaaatg ctttccgaga
    28141 aactgcttgc cacagacgct gtgacaaata cgtctttcat gtccaaacaa atcgaaacgc
    28201 aagaagaatc aatggcgaac ttagactcgg ttgtcttaat ctttgtcgta ttatcaggac
    28261 tgctggcttt tatcgtcttg tataatctaa ctaatatcaa tatttccgag cgtgtacgcg
    28321 aattatctac aataaaagtc cttggatttt ttgataaaga agtcacgatg tacattgttc
    28381 gagaaaatat catctttact cttttaggaa tcatcggagg atttggtgta ggatatatat
    28441 tgactgactt catcttgcaa caagcgtcta tggagaatgt catctttcca ttagtgatca
    28501 catggtgggc ttatgtctta tcagcaggat taactatcgt atttacggtt attgtgatga
    28561 ttgttaccca tttcaaatta aagcatatcg atatgattga tgcgttgaaa tcgaatgaat
    28621 aaaaacagag gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggcaaaa
    28681 tagtttttca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacca
    28741 tgaatcagag gttgtaaaaa agccgtcctg catcgagcaa aaagaatact caaataaaat
    28801 tagctcctca tgttttacat ttgagaagac tgattgccgt agataaaagc ttttgaacac
    28861 catttataga agaaacaaga atattttgct attttaaaca agaggataga caaatacatg
    28921 tctgatctct tgttttttat tgggcaacag acctcttaga atgtataaag ataaatttta
    28981 caaacgcttt cggttcagtt ataataataa atgataactt gtatatataa gttgtggtag
    29041 gtaataaatc agttgaatgg aggtaaatat ccatgacatt taaagacaaa gtcatttacc
    29101 aaatctatcc caaatcttac aaagacagta atggtgatgg aattggagat ttaaaaggaa
    29161 tcaaagaaaa actcccgtat ctgaatgatc taggcatcga catgatttgg ctaaacccta
    29221 tttatccaag ccctcaaaaa gacaacggtt atgatatctc tgattacaca gccatcgatc
    29281 ctatctttgg tacgatggag gaattcgaac agctaatagc cgaagcaaag aaataccgga
    29341 tagaaatcat gctcgatatg gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa
    29401 aagcactagc aggggaccag cattatcagg acttcttcat tttaagagaa gagccaacgg
    29461 attgggtatc aaaatttggt ggaagtgctt ggtcgccatt tggagatacg gggaaatatt
    29521 atttgcactt atatgatcgg acacaagctg acttgaactg gcggaaccaa gcagtaagag
    29581 aggaactatt caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg
    29641 atgttatcaa tgttatcggt aaagacgaac aattgaaaaa taataaagaa aatgatggca
    29701 aacctgaata tacggacaaa ccaatcaccc atacgtatct aaaagaattg aaccaagcaa
    29761 cgtttgggac ggattccact atcatcacag tcggtgaaat gagctctacg acgatcgaaa
    29821 attgtatctt gtatacagaa cctagtagag aagaattatc aatggtcttt aatttccatc
    29881 atttgaaagt cgattatgaa aatgggaata aatggtctac tcctccattt gattttgcag
    29941 aattaaagcg actatttcat acatggggag aaaagatgag tgagggtaac ggctggaatg
    30001 ctctattttt taataatcat gatcagccac gtgcgctcaa tcgatttgta gctatcgatc
    30061 agtatcgaaa acaaggagca gagatgctag caaccatgat ccatttgaac cgtgggacgc
    30121 cctatattta tatgggagaa gaaatcggca tgatcgaccc agactattca tcgatttccg
    30181 actatattga cgtagaaagt ttgaatgctt ataagttact ccttgaacaa ggattcacag
    30241 aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga taactctcga acacccatgc
    30301 aatggtcaga cacagaacaa gcaggattta ctacagggaa accttggtta aaagttggag
    30361 gcaagctaga agagattaat gtagaaaaag aactatcttc tgaagattct attttgtcct
    30421 attacaagaa attgattcgg ctgagaaaaa catatccaat cgttgcacaa ggagattatc
    30481 atgcttatga agcaaatcat ccggaagttt atggctttct tcgccaattt gaaggacagc
    30541 agctgcttgt attaacaaat ttctatgata aagatacaag gatcgccatc ccagaaggat
    30601 tcacagaagc caatattctt atcgataatt attccgatac tgttcttcat caaagtctgg
    30661 agctaaaacc ataccaggca attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc
    30721 gttcctacga gctattctcg taagatcgac gttttgtgtt ttatttaaaa taatcttctt
    30781 cagaaaatga agagttttca tactctcctt tatcgccata aaatttattg tagcgttgtt
    30841 tgaatttacg gaaaataaaa gtaacgagta atttgactaa cgtatagata ggaataccaa
    30901 aaataacgcc cacaattccc attagatcac ccatgacaag taaaacgatt aagatcgtaa
    30961 tcggatggat ctgtaaacga tcacccatta ctcgaggaat caccaagtca ccgtggagaa
    31021 gttgtacggc aaaccagaca atgacaaatt tgacagccat aaaagtagag tcttggaatg
    31081 caatgatcaa acctggaaag aaggcgataa acggcccaat atatggaatg atacataaca
    31141 acccagcaga aatagctaaa acactagcaa attctaatcc gatcagtaag aaacaaaccc
    31201 agtagactgc tccaaggata aatgaagcaa tgatttgccc ctttagaaat gaacccaatt
    31261 gttgattagc aatctttgtc aaatttttaa aatctgtacg aaatgctggt gggacaatcg
    31321 ctaaaactga ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg acaattggac
    31381 cagtaaataa agtaataaaa atagctgtaa cagttgaaaa aatgttgctg atccgttgtg
    31441 cgccactttc ccaataattt ccaataaatc cagctatatc agacgtcacg tcgtcaatag
    31501 aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac
    31561 tctcttgaaa atcttgaaac aaatctggca aactactgat caatgtcttt gcctgttctt
    31621 caagaaaagg aataagccat atgcttccaa gccccaataa aaataaaata atcacataaa
    31681 cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg actggttcca
    31741 aaagataata caagatcagt gcaaacaata atgggggcaa gatagtagcg ataatgattc
    31801 cgacaggctt gaatataaac gagatctgat ggaaaaaata aatgatcagc gcaagtaaga
    31861 taactattcc taaaacatag taagaggttt ttcctcctaa aaagcggatc aaacgattcg
    31921 ttgtatctgt ttcttttttc actctttttc ctcctttatc tataacttca ttatagacaa
    31981 aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca atcattcata
    32041 taaactttct catcaattaa aataagaaaa cgcttgacag ttcttttaac catatgataa
    32101 attaaatcct gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat cggaacattc
    32161 aaatgaaaga agatgagaag aggagtgtca taaatgaaac gattgatagg tatattggcc
    32221 atgctggttt tagcaggtat gttaactgct tgcggggcaa gtgggaaagc cgaggacagt
    32281 aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt atgattttac aaaagcaatc
    32341 gtcggggatg aaggagaagt agagttattg attccagcag ggactgattc ccacgattat
    32401 gaaccttctg ctaaagacat ggcaaagatc caagatacag atatctttgt ttataatgat
    32461 gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat tacaagaagg caatgtccat
    32521 acgataaaag cgacagaagg tatgctttta cttccgggaa gtgaagaagg acatgatcat
    32581 gaccatgaac acggggaaga aggccataca catgagctag acccacatgt atggctagct
    32641 ccaagtttag caatcaagca ggtagcaaac atacgtgatc agctgattga agcttaccca
    32701 gaaaaacagg aagtatggac aaaaaatgca gcagcctata ccgagaaact tcaagcactc
    32761 catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat
    32821 gctgcattca actatctagc gttagaatat ggattgaatc aagtatcgat tgctggtctt
    32881 tcttctagtg aagaaccgtc tgcggcgaga attgctgaac tgaaacactt tgttaaagaa
    32941 catggaatca actatattta ttttgaagaa aacgccaaag atagtattgc cagaacgtta
    33001 gcaaacgaag caggtgtatc tttagaagta ttgaatcctt tggaaggatt gacgaatgaa
    33061 caaatagaaa atggagaaaa ctatctttct atcatggaag caaacctcga agcgttgaaa
    33121 aaaacgacag agacagaaaa cccgctagaa gatactttga cccccaaaaa agaaaagagc
    33181 gtctacaacg gttattttga agacgaggat gtacaagatc gctctctttc agattgggca
    33241 ggaacttggc aaacagtaga tacgtatgta gaagatggga cttttgatcc tgtttttgaa
    33301 tataaagaaa aattgaatca ggataagaca gcacaagaat ataaagaata ttatacgaag
    33361 ggctatcaaa cagatattgc ttcaatcaag atcagtgaga aagagatggc atttacgttt
    33421 aagaatggaa caacgaaacg ttcaaattat cgttatgtag ggaaagaaat cttgacctac
    33481 gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag aagataagga aagtggcgct
    33541 tttagatata ttcaattcag tgatcacacg attgcaccgg ctgactccga acattttcat
    33601 ttatatgcag gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca
    33661 ttttatccag atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag
    33721 gcaaaaaaga accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt
    33781 tctttttatg aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt
    33841 gccaacgtga cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca
    33901 taggatttat gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca
    33961 tagaattgta attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa
    34021 gtagaaaaat attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt
    34081 ttgaagatat ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata
    34141 tgctcgacat tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg
    34201 atgttgcttt ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt
    34261 cctaaaggat ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct
    34321 tctagttcga tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg
    34381 agaagttctt ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg
    34441 attctatttt tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata
    34501 taaatgggaa tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa
    34561 cgctggtttt caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc
    34621 aagataaaca agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt
    34681 ttcaactggt ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa
    34741 tgattcttct tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca
    34801 atcgcattgt ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc
    34861 agttcgattg gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg
    34921 agttttaatg acagtaaact acggatctca ggaactttca tattatccaa catacttaga
    34981 ttggccggaa aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac
    35041 cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg
    35101 taatcatgtt tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc
    35161 tgttgcatcc tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc
    35221 caataataaa ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc
    35281 aaagctaaga catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat
    35341 gtaaataata tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa
    35401 gtctctaacg atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca
    35461 atgtgaaaat actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa
    35521 tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aagaaagcgc
    35581 gtaaaccaat tgttgacgcg aagtcgaaaa gaagaaaaaa gtaagcatca gcattcctat
    35641 agcaattaga gcagtaaaaa tccctaaaat cgaatagacc catccaatca aaatacaaga
    35701 aatcagaatg actcccgcaa acagatattt gtatcggatg tgatggtcaa tcaataagat
    35761 acaaattgtg taaatcaaat attgcaacgc tatggaccat aaaaacataa agtacagctc
    35821 ccattgattt ttaccaaatt atagcacaat tggcgtatta tttttttaat ttttcaggga
    35881 ttttgaattc agaactcata ccaaagcagc agccagatac aaaaaagtca gataagttag
    35941 ttaattgttt aatgattgtt tttttcatga aagattcctc caaaattcat caagtagttt
    36001 gtaataggaa gcaacgtagc tatttctaaa acaatcccaa gtagcaaata ggtagaaaag
    36061 taagaagctg agaaaaataa cgtaatgaga gaaaaaatta gaaacatgct gatcgccttc
    36121 tttaataatg ggggacgttt ctcgggttca atgggattga ttgctgtacc tcttgatcca
    36181 tagcgaagca gtaacaaaaa gcagctgatt aacaaaaaga ccatcattgg gaaggagata
    36241 gtcaattgca gatgtttcat caccaaggga acgccccata aataaagtag tgtccacagc
    36301 agacaaacga attcagagtc ggaatgcaat ccataagcat atctgcggat gcagatataa
    36361 gaaagaaagg tgattcctgt ttccgcccat gagcctgtca ccagagaaaa gactagaacg
    36421 accgctagtt ttccaagatt attcaataaa acttctaaga caaattttac ttggacatac
    36481 accatatctt cttccgattt gtctccgctg aataaggctt ctaataattt gttcacaact
    36541 ttatctttca tctcatacgt acctccacca caaattttat gctaaaaaaa ataaaaaacg
    36601 tcttttgttt cataaaaagt ggttttgaca tcaaaaccgt catgatctcc taaaaaaatc
    36661 ttcgttttcc aagaacctta ccaaaaaaag tgacagtttt gatacgaaaa gtacttttta
    36721 tgacaagttt gtgctttttc gagtgattta tgttaaaaaa ttactgacct tataaaaggt
    36781 gatggaggga ttcttatgga aaatgaacaa ttgtttgaag tcggcgaaac atatgcattt
    36841 aaatctgatt tatttgaatc accaataaaa ggaaaaatag aaaaaatcta tgaaaatagt
    36901 gtgatgatac gtgtcatgaa atgcgcagta gaagacaatg ataccgctca taatctgcac
    36961 tggcagttag ttgtacgcaa atcaagtaca atagaagaat aaaaacagat aaaatgttga
    37021 gagcataaag aggttgtgag tctcccgttt agctctgagt tacaaggaat aatgttaaaa
    37081 aacagctttt catgttttat gaatattgcc ttgtgaccga ggtgttggca ggagaatact
    37141 gtttatttga aaaaagggct gcgacaagac ttttgtcaca gcctttattt tcatgaatag
    37201 gagttacttt tggctacttt tagagaagta gcttttaagg agcagataaa gtcaaggctt
    37261 ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga aggatgccga
    37321 taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacacta actgaaatgc
    37381 ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat gaagtattgc
    37441 cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc tttttgaaca
    37501 aacgcttttt ggcaatcaaa gatgctttca gccatgatga caacgtacta gaccgctcca
    37561 tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt tctgaagtag
    37621 aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa gaaatttcgc
    37681 cacaaaagaa accagatcta ctggtttata ttcgtgtatc ctttgaaact atgttagcga
    37741 gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa ctctattcct
    37801 actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat atatgtccca
    37861 aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt ggagaacaaa
    37921 tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat gcccagtcga
    37981 tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa taatataaaa
    38041 actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc ttagtttttt
    38101 tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat aaatgaaatg
    38161 tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa tgggcgccaa
    38221 acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga acgacactta
    38281 gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa tcatataagt
    38341 tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca cgcccgttga
    38401 ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc gttactaatg
    38461 cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct tcataatata
    38521 gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt agatactttg
    38581 ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca acacttagcc
    38641 attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga gcagaggcaa
    38701 aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat gtcagcaaaa
    38761 gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc ttttatgaat
    38821 atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt ttcagaaaac
    38881 taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat
    38941 agacaacgaa actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga
    39001 aggaaatcaa tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct
    39061 gggagaacaa gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta
    39121 cctatgaagc ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag
    39181 aaagtgaagg aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca
    39241 atcaagaaca aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag
    39301 gagctgggag cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa
    39361 aacaagtaga cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac
    39421 taaaaaaacg agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc
    39481 atagtatctt ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc
    39541 aaggaataca aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac
    39601 agctgatacg taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt
    39661 ctcggttaga ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta
    39721 aagaaaaata tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg
    39781 gattgctcac ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg
    39841 tactccaaaa tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa
    39901 aatatgatta tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg
    39961 tgaaacggat gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga
    40021 gtatctatgg attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc
    40081 ctaccaaaac tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag
    40141 caaatagatt gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac
    40201 aaaaagaagc aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt
    40261 ggctaatcaa acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca
    40321 tattataccg atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata
    40381 ttccttttgt actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc
    40441 aacatttcta tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc
    40501 aggcctacca gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat
    40561 gcgatcaata cttttctcct aaatatcaaa atatatccgt tgcagattat attttgtcga
    40621 taagccccaa tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta
    40681 aaacagacca gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta
    40741 tagctaagga aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg
    40801 ctactttcag aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa
    40861 aaacccgctt gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc
    40921 acaaaagtaa aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt
    40981 tgccttataa cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac
    41041 cagtaacgac gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa
    41101 atcgtctgta tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat
    41161 ttatcaaaga aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca
    41221 gaagtggtca gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt
    41281 gaaatttcag cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga
    41341 ggtgtgacaa gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat
    41401 caaaatagag aatatataga aaaattctca ttattcaaac gtaaataatc agttattttg
    41461 atttttttta tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg
    41521 gattataatt aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt
    41581 cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata
    41641 tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag
    41701 gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta
    41761 acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac
    41821 gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt
    41881 catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata
    41941 acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac
    42001 tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt
    42061 gttgacttag aagaacgcga tctaagtaca gaagaagcag aagaattatg cagttttgaa
    42121 gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt
    42181 gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc
    42241 ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg
    42301 atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt
    42361 tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac
    42421 tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg
    42481 ggatatagag ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt
    42541 gttttaagta gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca
    42601 tgttagaaat gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg
    42661 attctatcga aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata
    42721 aatgagttgc tgtaagattt cgaaaaacga aatcaaaaaa acttttaaaa aagagttgac
    42781 aattacttgt ctctctgata tgatataaaa gttgctacgg cagccaaaaa cgaatcggg
//
LOCUS       DACEMS010000010        40917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEMS010000010 DACEMS010000000
VERSION     DACEMS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.010"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            139..786
                     /locus_tag="IW735_000485"
     CDS             139..786
                     /locus_tag="IW735_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1043508.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1179..3824
                     /locus_tag="IW735_000486"
     CDS             1179..3824
                     /locus_tag="IW735_000486"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1043509.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4134..5447
                     /locus_tag="IW735_000487"
     CDS             4134..5447
                     /locus_tag="IW735_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1043510.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5437..6090
                     /locus_tag="IW735_000488"
     CDS             5437..6090
                     /locus_tag="IW735_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1043511.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6145..6816
                     /gene="radC"
                     /locus_tag="IW735_000489"
     CDS             6145..6816
                     /gene="radC"
                     /locus_tag="IW735_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1043512.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6711..8063)
                     /locus_tag="IW735_000490"
     CDS             complement(6711..8063)
                     /locus_tag="IW735_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1043513.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8129..9418)
                     /locus_tag="IW735_000491"
     CDS             complement(8129..9418)
                     /locus_tag="IW735_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1043514.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9505..10209)
                     /locus_tag="IW735_000492"
     CDS             complement(9505..10209)
                     /locus_tag="IW735_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ1043515.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10409..10675
                     /locus_tag="IW735_000493"
     CDS             10409..10675
                     /locus_tag="IW735_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1043516.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10843..11073)
                     /locus_tag="IW735_000494"
     CDS             complement(10843..11073)
                     /locus_tag="IW735_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1043517.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11145..11300
                     /locus_tag="IW735_000495"
     CDS             11145..11300
                     /locus_tag="IW735_000495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043518.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11609..11833)
                     /locus_tag="IW735_000496"
     CDS             complement(11609..11833)
                     /locus_tag="IW735_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1043519.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            12100..12435
                     /locus_tag="IW735_000497"
     CDS             12100..12435
                     /locus_tag="IW735_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043520.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12668..13609
                     /locus_tag="IW735_000498"
     CDS             12668..13609
                     /locus_tag="IW735_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1043521.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            13611..14501
                     /locus_tag="IW735_000499"
     CDS             13611..14501
                     /locus_tag="IW735_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1043522.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14544..15344
                     /locus_tag="IW735_000500"
     CDS             14544..15344
                     /locus_tag="IW735_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1043523.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15359..16210
                     /locus_tag="IW735_000501"
     CDS             15359..16210
                     /locus_tag="IW735_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1043524.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16207..16566
                     /locus_tag="IW735_000502"
     CDS             16207..16566
                     /locus_tag="IW735_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1043525.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16581..16742
                     /locus_tag="IW735_000503"
     CDS             16581..16742
                     /locus_tag="IW735_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1043526.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16739..17065
                     /locus_tag="IW735_000504"
     CDS             16739..17065
                     /locus_tag="IW735_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043527.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17062..17877
                     /locus_tag="IW735_000505"
     CDS             17062..17877
                     /locus_tag="IW735_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ1043528.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            18016..18327
                     /locus_tag="IW735_000506"
     CDS             18016..18327
                     /locus_tag="IW735_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043529.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18324..18554
                     /locus_tag="IW735_000507"
     CDS             18324..18554
                     /locus_tag="IW735_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043530.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            18561..18764
                     /locus_tag="IW735_000508"
     CDS             18561..18764
                     /locus_tag="IW735_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1043531.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            18761..19057
                     /locus_tag="IW735_000509"
     CDS             18761..19057
                     /locus_tag="IW735_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043532.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            19134..19547
                     /locus_tag="IW735_000510"
     CDS             19134..19547
                     /locus_tag="IW735_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1043533.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            19560..19946
                     /locus_tag="IW735_000511"
     CDS             19560..19946
                     /locus_tag="IW735_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043534.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            20497..20721
                     /locus_tag="IW735_000512"
     CDS             20497..20721
                     /locus_tag="IW735_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043535.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            20739..21167
                     /locus_tag="IW735_000513"
     CDS             20739..21167
                     /locus_tag="IW735_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043536.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            21145..22563
                     /gene="terL"
                     /locus_tag="IW735_000514"
     CDS             21145..22563
                     /gene="terL"
                     /locus_tag="IW735_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ1043537.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            22575..24110
                     /locus_tag="IW735_000515"
     CDS             22575..24110
                     /locus_tag="IW735_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1043538.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            24190..25068
                     /locus_tag="IW735_000516"
     CDS             24190..25068
                     /locus_tag="IW735_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1043539.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            25070..25387
                     /locus_tag="IW735_000517"
     CDS             25070..25387
                     /locus_tag="IW735_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043540.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            25436..26137
                     /locus_tag="IW735_000518"
     CDS             25436..26137
                     /locus_tag="IW735_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1043541.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            26152..27042
                     /locus_tag="IW735_000519"
     CDS             26152..27042
                     /locus_tag="IW735_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ1043542.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            27065..27280
                     /locus_tag="IW735_000520"
     CDS             27065..27280
                     /locus_tag="IW735_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043543.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            27292..27633
                     /locus_tag="IW735_000521"
     CDS             27292..27633
                     /locus_tag="IW735_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043544.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            27633..28004
                     /locus_tag="IW735_000522"
     CDS             27633..28004
                     /locus_tag="IW735_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043545.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            27997..28392
                     /locus_tag="IW735_000523"
     CDS             27997..28392
                     /locus_tag="IW735_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1043546.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            28394..28771
                     /locus_tag="IW735_000524"
     CDS             28394..28771
                     /locus_tag="IW735_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043547.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            28772..29380
                     /locus_tag="IW735_000525"
     CDS             28772..29380
                     /locus_tag="IW735_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1043548.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            29380..29721
                     /locus_tag="IW735_000526"
     CDS             29380..29721
                     /locus_tag="IW735_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043549.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            29963..32752
                     /locus_tag="IW735_000527"
     CDS             29963..32752
                     /locus_tag="IW735_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1043550.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            32742..33482
                     /locus_tag="IW735_000528"
     CDS             32742..33482
                     /locus_tag="IW735_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1043551.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            33479..36241
                     /locus_tag="IW735_000529"
     CDS             33479..36241
                     /locus_tag="IW735_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1043552.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            36254..37162
                     /locus_tag="IW735_000530"
     CDS             36254..37162
                     /locus_tag="IW735_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ1043553.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            37162..37779
                     /locus_tag="IW735_000531"
     CDS             37162..37779
                     /locus_tag="IW735_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043554.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTNERIPNFTFEKIGEV"
     gene            37783..38232
                     /locus_tag="IW735_000532"
     CDS             37783..38232
                     /locus_tag="IW735_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043555.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLTNITFEKVGTV"
     gene            38232..38756
                     /locus_tag="IW735_000533"
     CDS             38232..38756
                     /locus_tag="IW735_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043556.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            38784..39191
                     /locus_tag="IW735_000534"
     CDS             38784..39191
                     /locus_tag="IW735_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043557.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            39191..39328
                     /locus_tag="IW735_000535"
     CDS             39191..39328
                     /locus_tag="IW735_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1043558.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDEEADKIISKEESA
                     S"
     gene            39366..39659
                     /locus_tag="IW735_000536"
     CDS             39366..39659
                     /locus_tag="IW735_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043559.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            39656..39880
                     /locus_tag="IW735_000537"
     CDS             39656..39880
                     /locus_tag="IW735_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1043560.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            39877..40902
                     /locus_tag="IW735_000538"
     CDS             39877..40902
                     /locus_tag="IW735_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1043561.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVDGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPQR"
BASE COUNT        14207 a         6275 c         8520 g        11915 t
ORIGIN      
        1 gagcttatta ccgcagatgc aagcttttga acaccgtttt atttgtctca cacccttttc
       61 cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt acactaaata tgtgaaatta
      121 ttaacaagga gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc aactgcgaaa
      181 agattacctt tatatttacg ctacttgaag atgctggatg actcaggtat aagtagaatc
      241 aaatcaaatg agttcagcga aatcacccaa attccttctg caacgatccg cagagacttc
      301 tcgcaattag gcgagctagg cagaagcgga tatggctatg atgtcccttt tcttatcgat
      361 gtattcaata atatccttaa tactaaagaa gaaaaacgaa tcgcattagt aggatatgga
      421 aatcttggca aagcattgaa acacaacaat tttagaagaa atgaaaatct aaatatcgtg
      481 tgcgtctttg ataatgatcc agcactgatc aatcgagtaa ttgatggaga aatgatctat
      541 ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc aatctctacg
      601 gtaccaagta aatattcaca atctgctatt gatgaaattg tcaaaggaaa tgtaaccgct
      661 atacttaatt ttgcgcctga cagagtaact gtcccagctt atgtcaatgt tcaatacatt
      721 gatttgacaa ccgagttgca gacgcttatt tactttgatg agaattactc agaagttttt
      781 tcttaggaaa atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa ttctttcaaa
      841 aattttttag tactaaatga agtggttttt tcacgaccac ttgttgacta aaaaaaaaaa
      901 ccattgtaga atgaagcata acgctgaaca agaggagtaa ctggcacatt cattcttaga
      961 gagagaacta tctgctgaaa ggttctcaat gacaaccagt gaaggtagct tgggagtttc
     1021 tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta actcacttga gaggcaataa
     1081 tgattgatta ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt
     1141 attttaggac gttttttagt ttaaaggagg aaaacagcat gacggaagaa aataacctat
     1201 caacaaaata taatccgcaa gaagttgaag ctggacgtta tcagcattgg ttagaacaag
     1261 atttgtttaa accgagcgga gataaaaaag caaaacctta ttcaatcgta atcccgcccc
     1321 caaatgttac tggaaaactt catttagggc atgcttggga tacgaccttg caagatatgc
     1381 tgattcgtca aaagagaatg caaggatatg atactttatg gctgcctggt atggaccatg
     1441 caggaatcgc gacacaagca aaagtagaag cgaagcttag agagcaaggg atttctcgtt
     1501 atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa gagtatgcga
     1561 gccacatccg cgaacagtgg gctaaaatgg gcttatcttt agattacagt agggaacgtt
     1621 ttacattaga tgatggatta tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa
     1681 aaggtttgat ttatcgtggt gaatatatca tcaactggga tcctcaagca cgaacagctc
     1741 tatctgatat cgaagttatt cataaagata tcgaaggcgc cttttatcat atgagctatg
     1801 aactagcaga tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg
     1861 gagatacggc gattgctgtt catccagaag acgagcgtta tcaagcgctc atcggcaaaa
     1921 aagtgattct acctttagtc aataaacaaa tcccaatcat tgcagatgaa tatgtagata
     1981 tggaattcgg gacaggtgta gtaaaaatca ctccagcaca tgatccaaac gactttgagg
     2041 taggtaatcg tcatgatctt ccacgcgtaa atgttatgga tgatgatggt acgatgaatg
     2101 aactagcagg aaaatatgct ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag
     2161 atctgaaaga aatcggtcga ttgattaaaa tcgatacgat gatccacagt gtgggtcatt
     2221 ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg
     2281 cgccgttagc gaaaaaagcc attgataacc aagaaacaga tcaagcagtg gaattcttcc
     2341 ctccaagatt caatcaaacg tttttacgtt ggatggaaaa tgtccatgat tgggtaatct
     2401 cacgacaatt atggtgggga catcaaattc ctgcatggta tcacaaagaa acaggtgaaa
     2461 tgtatgtagg gatggaagca ccagcggata gtgaaaactg ggtacaagat cctgatgttt
     2521 tagacacttg gtttagttca gctttgtggc ctttctcaac aatgggctgg ccagatacgg
     2581 aaagcgaaga ttataagcga tacttcccta ctagcacttt agtaaccggt tatgacatca
     2641 ttttcttctg ggtaagcaga atgattttcc aaagcttgga atttactggc gaacgtccat
     2701 ttgagaatgt cttgattcat ggattgattc gtgacgaaca agggcggaag atgagtaaat
     2761 cattaggtaa tggaatcgat ccgatggaag taatcgaaaa gtatggagca gatgctctcc
     2821 gctggttctt atcgaatggt tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa
     2881 tggatgcttc ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc gttatcatga
     2941 atatcgaagg aatgaccgta gaagagatcg atttatctgg agaaaaatca gtggctgacc
     3001 ggtggatcct tactcgtttg aatgaaacga tcgaacgagt aactgagcta tttgatcgtt
     3061 ttgaatttgg tgaagcaggt cgacaactgt ataactttat ctgggatgat ttttgtgact
     3121 ggtacatcga aatgagtaaa gaagtacttt atggagatga tgctgtttca aaacaaacca
     3181 cccgtagcat cttagtccat acacttgatc agatcctacg tttattgcat ccaatcatgc
     3241 cattcgttac agaagaaata tgggaacata ttcctcatca gggaaattct ctagtagtgg
     3301 cagaatatcc tgttgtacgt ccagagtttg atgatgaaac agcttctaaa gggatggaag
     3361 tattgaaaga attgattcga gccgttcgaa atattcgttc agaagtgaat acaccattgt
     3421 ctaaaccaat tacgttgcta attaaaacaa acgatgctaa aatcgatcaa tttttagttg
     3481 aaaatacaag ctatatcgaa cgcttctgta atcccgaaga gttgacgata tcaagtgaga
     3541 tcactgcgcc agatctagcg atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt
     3601 tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt
     3661 ggacaagcga agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt gtttctaacg
     3721 cgccggaaga agtagtagag gcagagcgtg caaaagaaaa agattattta gagaagcaaa
     3781 cggctgtcaa agaacgaatc gaaagtttgc gtactattca ataagaatag aaaaagagag
     3841 tgtgaaatca gctagctatt actaaaagct agctgattga acatcgttac aggaaagagt
     3901 ttgagcctca ctcttagctt cgattcataa ggagatgtga cataaatttt gtcgcatctc
     3961 ttttttagtc tactcttatt atgtaggaga aggagggagg tggatcatgt atcaagagtc
     4021 aataaagtag ttgataaaat cgttcctctc tctgtttctt ttctctttgt catactttta
     4081 tttttcttat gtgttctatt tgttataata aaagagaaat gggggaattg ttgatgacga
     4141 ttgaagaagc aatagaatgg atccatagtc gattaccatt tggttcacgt cctggccttg
     4201 accgagtcga agctttatta gaagtggtag ggcatccaga gagaaaagta aaaacgatcc
     4261 atatcgctgg aacaaacgga aaaggttcta ctgtagctta tttgagaagc ctattagaag
     4321 aggctgggct gacagtaggt acatttactt caccttatat cgaaacgttc aatgaacgga
     4381 tcgcaatcaa tggcaaaagc attccagaca atgatttgat ccagcttgta aaaaaatatc
     4441 agccactgat caaacaactt gatcaatttg aagcagtagc tggtattact gaattcgaga
     4501 cattaacagc aatggcattt gattattttt tgatgaagaa agtggacgta gcgattatcg
     4561 aagttggctt aggcggctta ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa
     4621 taacaacgat tggacttgat cataccgaaa tacttggaga tacaattgaa gaaatcgctg
     4681 cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt aacaggaaat attgccccag
     4741 aagcttttac tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg
     4801 gggaaaatta taaaataaac tatctgcatc ctgatgagat gtggggagaa gtatttgaat
     4861 tttttaatga aataggaaag atccctcaat taaccactcc gcttttaggg caacatcaag
     4921 ctgaaaatgc aggagtagcc atcgaattgt attttgttta ctgtcaattg gaaaagctgc
     4981 ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg
     5041 agcgtttgag taacgagcca ttagttgttt tagatggtgc ccataatacg catgcaatga
     5101 accggcttgt tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac attttgtttt
     5161 ccgccttacg aactaaaaat gttagagaaa tgctagaacg attgcagcaa gtaccaaatg
     5221 tccatattta tttgacgacc tttgaacatc caaaagccat cgaattaagt gaagtatcag
     5281 atttagcaag tgagagagta tcaatcgttt cattgtggca attcggatta gcagatatat
     5341 tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg ttcactttat ttcatttcag
     5401 aagttcgcca tttattaata caattaggag gagatcatga agtataatgc agtgattttt
     5461 gacatggatg gtttgctgtt cgacacagag ttagtgtatt acgaagccac acaagtagta
     5521 gcagacaaga tgggttttcc ttatgataaa gacctgtatt taaaattctt aggtgtttca
     5581 gatgaagaag tatgggcgaa ttatcatatg attttctcgg aatttggcaa aacaagtgta
     5641 caaagattca tagatgattc ataccaggaa acgcttgaac gtttttcgca aggagcggta
     5701 caaatgaagc ccggtgtgtt ggaactcctc tcttttttag aagaacatca gattcccaaa
     5761 gttgtcgctt ccagtaacca acgagcagtc attgaacttt tattgaagaa gaacaatctg
     5821 tttgaacgat ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa accagaccca
     5881 gaaattttct tgttagctaa tcagaagcta ggtacagcga agaaagaaac attgatttta
     5941 gaagattcac aaaatggtat tttagcagct tctgcggctg agatcccggt agtcatggta
     6001 ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa cagctggggt tgtttcttct
     6061 ttacatgaaa tccctgcatt gttaaaataa aaatccatct cttttcgtat cttattttag
     6121 ataagataga aaggagatgt ttttttggga aaacgattga tcaaagaagt acctactagc
     6181 tctttaccaa gggaacgtat ggaaatttat ggcgcggaag ctttatcgga tcaggagctt
     6241 ttggcaatct tactgcgtac cggtcaacac ccttatagtg tgatgtcgat tgctggaaat
     6301 ctgttaaaaa cgtttggagg actggcctct ttacgacagg ctactttgca tgagttagaa
     6361 gagattcgag ggataggacg cgtgaaagca atcgaaatca aagccttgat cgaattaggt
     6421 cgacgaattc agacggatca tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg
     6481 gcccagcaat tgattctgga aatgaaagat cacaaacaag aacatttggt ttgtatttat
     6541 ttggacacga agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag
     6601 tcagtcgctc atcctagaga aattttccat tatgctgtac gttattgtgc agcaaggatc
     6661 gttttagcgc ataatcatcc ttccggaata tataagttag tacacataaa ttatataagg
     6721 aaatttactg ttatattgcc atctggaaat actattatgt tatcgataat tgaacgccat
     6781 aatgcatttt tttcttcttc cgaagattct ttataaattt catcgaattc ttttgataga
     6841 ataaattcga cattttttat atctatttta gctggctcta cttcaatttc atttaattct
     6901 ttcattaaag cttctcgatc ttttttgtat tcctctaagt ctataacttc attcaagtag
     6961 gctttcttca atctatctaa tttattctca atatttttct tactattttt ctttttaata
     7021 atctttggtt gtttttttga atagtttatt tgtatttgtt caaatttttg tttagtagct
     7081 tcaaaaatat aacgttctaa atttttttcg taaaaagcgc gcttatttgt gcaacgtaat
     7141 ttattatttc ttcgttttac acatctataa caagtccttt cgtatataga ttctgtaccg
     7201 tcttttttct tatatcttgc tttaatagtt tgaccagcta ctgatccctg acagtcagaa
     7261 cattttaata aacctttaaa aatgtaatca cgttttgcat ttgtttttaa atttttaggt
     7321 aaggcttttt gaacagcata aaaaagtttt ttatcaataa taggaggaca gtaattttta
     7381 ttatttctta attcaccaat gtattttttg ttcgtcaaca tatttttaac actttgataa
     7441 tctctaacga ttccatactg agagcctaaa taataaacag tttttcttaa actaccacta
     7501 gaaagaaagt agttgaatat atctatcacg ataggagcat catcatttat tactaatttc
     7561 ttattttcta tagagtagcc taacggagtt ttcccgctta ctacttcacc ttgtgcaatt
     7621 ttatttgcca taacagcttt gatgcgttct gaatcaattt gggcttccaa ttcagcaaaa
     7681 ctcattactt gtgcaatgaa agttctgcca tacgctgtag ttgtatcata atattgttga
     7741 gaaacagcat tccaagaaac attgtgtttt tctaaaattt cttgagtgtt taaataatgt
     7801 cgtagatttc taaaccatct atctaatttt gtaaataaaa ttagatctat tttattttct
     7861 tttacatctt gtaatagttt ttgaaattca tcacgattta ttttttgacc agaaatacca
     7921 ccgtctacat attcattaac tactttcaaa tccttgtgtt gttcgatata ttcatagagg
     7981 gtttcttttt gttctctaag actatctcca tgtttggctt gttgttcagt tgatactctc
     8041 atatataatg ctactcgttt catgttatct cctttcgaat gtatgttctt atcgttgtaa
     8101 aagaaaagcc cgaaggctaa tctttttact acacatctgt taattctaat gatcttctgt
     8161 attgttcagc ttgagctagt ttagtaaaag atacagttcc gttataattt tctaaaacaa
     8221 ctttttcaat ttcatcaaga gatactttaa agaattcttt tcgttgatta actttattga
     8281 cttcgttttt tctaaaatgc gaatgtagtt gattttctaa tgttggtgca tcttctgaaa
     8341 atatcattgc atgaacatca aattcaaaag gtacagaagc gctactaagt tccttaattc
     8401 tatccatagg ttctaaccgt ctagtcattc ctattttata gacatcttca ccgaaagatc
     8461 cgatattact gatgacataa acatagccag ctctagtatt aagttctctt tgcaatacat
     8521 cttctttgac agtttctagt tctcttaatt tctcttccag ttctttaatt ttatcagcat
     8581 agagttcttt ttcaacatca gcactagctt tttgcatgta tgccattaac ttagttatct
     8641 ctgagttaaa ctgtcgttct tctttatcaa tgcgtttctt ttcacgttct atttcacgtc
     8701 ttaccttttc ttcttcaatc atttgttctt ttattgcttt ttgctgttct ttttcatcgt
     8761 taactttaac tatgtatgaa tagttgagtt ctaattcttg aagttttaat tcaagtaatt
     8821 cttttttgaa ttctgcttca tcgtttttaa aaagcttgtt tattttatca aaagttctta
     8881 taattttttc tctagttgaa tctacgttag aaatagtaag tttattgaga agtccagtta
     8941 tttctgattc taatggtata agcattttct ttttaatatt attagcttct ctattattca
     9001 aataagaagg aatattaatt gcaccgtcta aaataaaatc cttttcttta atctttaata
     9061 aagaaagttt gttttttatt tcggatgaag aagtttcatc tatattcgga ataattactg
     9121 atttatagat agtgaccaga gatgtgtcat atttttgatt aatttcatcg tctaaccgct
     9181 ggctttcagc attaagaata tcaattttgt tttctagcgt tgacaagttt ttattcaatt
     9241 tacttatgtt tttctctttt tcagatataa acgtttccct agcatttaat tcttgagaat
     9301 attcctttac cttactctct aaggcagaag ttttatcttt taatttttta gcttcagata
     9361 atttgctact acccataatt attaaaacta ttccaactaa aaacaatagt actcccattc
     9421 aaaacgcctc gcttctttta taaaataatt gtcaaaatca tataaaccct taccgaagtt
     9481 gacggctccg gcatgggaat ttttttaaaa tacaactttt ccgataattc taatctgttc
     9541 tggcgataca attctatctt cgtataagtc gtttatagaa cgtaaaatga ttgtttgatt
     9601 ttcgtaatct ttatatatct ctttacatgt aactccgtca ccacctaatt ccacaatagc
     9661 aatttcccca tcttctacgt ccggttgttc tttaacgaat acgaattgtc cattcctgat
     9721 aacaggttcc attgaatcgc ctataatagg tactgcgaag tcagcagcac ttggaactct
     9781 ctcaaattct tcctcttcga caaccgtatc accgtaagta agttctgttg gattggcagc
     9841 cgaagccgaa ttgtgaacag ttgggatttt ttctttttta ggaaaatcta ggacattttc
     9901 ttgtttttgt tcttctaatc gattacttgc aaattcatag gtagcaactt tattttcgtc
     9961 gttaagctga ttatatatag gaaggatatt atatatctga ttagatatag ataaatcaat
    10021 attcatcaaa tcatctatat caacattgaa tatcttagct atctcattta aggtttttaa
    10081 ttttggtgta tattttcctt tttcccattc gcttattgag gatgaacttt ttcttcctag
    10141 tttttcggct agttcttgtt gatcaatatt atacttttct ctaagaaact ttagattttc
    10201 agcgaacata gtaacacctc cttgagttaa ttgtaacacc gtttcggaaa aaatgtaaca
    10261 ggtttcttag aaaatatttt agtgttttta aagaagtatt tacgcaaaat agaaatcgat
    10321 ttcaggaaaa ctgaaatatg cacttgactt cggaaaaacc gaagtatata atttagtcag
    10381 aaagttaaag agaggaggcg aataaaatat ggataccgcg aagaaagaaa aatatacatt
    10441 gagacaatta agagctttaa agggttattc aaaagaagaa ctgtcaagga aaagcaaagt
    10501 gacatcaaga acaatattta tttacgaaaa cgatgtagat aaaatgagga acggaaagta
    10561 cgccactcta gaaaaaattg ctaaggcatt gggtgttaga gtttccgata tttttttaga
    10621 ccctgattcg gaaaaaccga aatcaataac aggtgaaaca aaacaagtca gctaggaggt
    10681 gtgaatagtg gaagagaaga atataaaagt accgactctt gaagaatata caaaaattgt
    10741 tgtcgaaaca gacgagcaga acccgaaaac aatcgcagtt attacggcga atgatattga
    10801 atcatgcgaa ggttttcgag ttcgaatgac ccctagatat gattaatgtt ctttatcacg
    10861 tggaggtata ggatcgtttc caaaactatc ttttgattgt atttgtccat tctttttttg
    10921 gacaataaac tctgattgct gttttttaga aatcttttta ccatactcaa cagcatctga
    10981 tttattatca aatagttttg tatcttcgga attaccttca ccacgtacag cccatttgcc
    11041 atttctagga gatacccatt gatttttacc cataacaatt cacctcgctt tcaagttaat
    11101 tctattaaga ggtgaaaaga aaacaaactg atggaggtga gattgtgagt aacaagccaa
    11161 aaatcacttt gaatggcaca cctaatcttc aaagattagc agatgaaatg gctagaatac
    11221 gttctagaca ttggggagtt actgtggtgg caaccataat tgaacaaaaa gaaaagccca
    11281 cacagccggc aagcaagtag tgggcaccat agaacaataa tattatatca cacactcgcc
    11341 acttccgaaa agaggagttg ctgctctcct ttcaagtttg ccaaagtcca cgtttcttca
    11401 ttttggcaac tcctcttctt ggaggtggtg acggcgagag acgaaggagg gatgacatga
    11461 aggaagaatc aaaaataggg attttgaatg agaacaatta cacaagatta attattgaaa
    11521 cagaagaagg ggaaaaagta gcagaagtta cattgaccga tgcaactcct gctgacggtt
    11581 atcgtattcg attaactcct aaatatgatt aaccttttgg tgggtgtgga tcgtttccgt
    11641 gactatcacg gctttgaatt tttccatctc tgccatgaat aaatagctca gatccttgat
    11701 ttttagaaat ctctctagcg atttttactg cttctgattt tgtattagta tgtgcagtcg
    11761 ctttagaatt tccagcacct ttgacattcc aaccaccatt ggaattaggt acaacatgtt
    11821 gattttttcc catagttttc acctccattt ctatagaaat attttatcaa atagaaatgg
    11881 agaaattcaa taaacgaaag gagggatcaa atgacattac aagagcatca acacttgatg
    11941 cgaaaactaa atcaggaata ccacgaggaa atgaaaccta gattgctagg ggaacaatgg
    12001 cgtgaacgcc agaagaagtg gttagaaata aaaaagtgac tcagccgtcc aaagcaatga
    12061 gtcacaaacc aaaaattata cctaaggaga ttttatcaca tgaataagaa aattgaaaat
    12121 cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg tttctattat ttgtacagcc
    12181 caaaaagaag gggaacttaa aagtctcgtt tatggtgaga caacagaaat tttactttgt
    12241 cttgctatgc aagaagaaca tttagatgaa aatctcccat tgtcagcaca tattatgcgt
    12301 agaattgctg tggatgctta cgaacaagcg aaaaatgaag aagaaaatca accttcaaat
    12361 catacttttg ttattaataa caaggaagat ttagctgatg tgatgactcg tattctcaag
    12421 ggggaatttc aatgatgaat gaaaaaacaa ttgatctact cattatttta tttttcacgt
    12481 tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt attcgcaatt accatgcttg
    12541 tgctttgcgt gttagcaagc agaaaagagg ttaaacatga acgagttagc aaatcttgac
    12601 aactatttaa ctgatcctga ttatgctaaa ccaccatatg aagcgccaat tgatgaggag
    12661 gatgaagatg agtaaatcta ccttagaaat gagccatcaa gaatggctcg aagaccgcaa
    12721 aagaggtatt ggtggctcag atgttgcaac cgtacttgga ttaaacaaat acaaatctcc
    12781 ttatcaattg tggcttgaga aaacgggtca aattgaattg aaagatttag aaagcgaacc
    12841 agcttattgg ggtaatgttt tagaagaagt ggttgctaaa gagtttcagg aacgtacagg
    12901 caaaaaagta cgcagaagaa accaagtatt tgaacatcca ttacatccat ttttaagagc
    12961 aaatattgat cgggaagtag tgggagaaaa tgccattctg gaatgtaaaa cagccaatca
    13021 atttctcgga aaagagtggg aaggtgaaga ggtaccgctt agctatctct gtcaagttca
    13081 gcattacatg aacgttttaa acaaagacta ttgttatatc gctgttttga tcggtggaca
    13141 aaaattcatt tggaagcgga ttgaacgaga tcaagagctg atcgatacaa tcactgaaca
    13201 attagtagag ttttgggaaa cgaacgttct tggaggtatc gagcctgtaa ttgacggaag
    13261 tggagcgact gctgacttct taaaagaaaa atatgcagat gtagaagaaa atcaaacagc
    13321 tctaccatca cgttttgatg aacttatcga gcaaaaaaga gaactcaagc ggacgaaaaa
    13381 agaaattgaa tcatctattc gtcaagtaga caacgagatc atcagtgagc taggtaaacg
    13441 tgaagccagt atcggtatca cacaaagaaa cattatcagc tggaaacttg ttcgtacaag
    13501 acggatgaac acgaagaaac tagcagagaa atatccagat gtcgcaaatg atgaagagat
    13561 ttataacgtt actgaatcaa gaaggctaac cgaaaaggag atcaaataat atggcaacaa
    13621 atgaatcgtt aaaaaatcaa ttggcagaaa agccacagaa acaagttgca ccaggacagt
    13681 tagggcttaa agctctaatg aatacaccaa caatgagaaa gaaatttgaa gaagtacttc
    13741 atgacaatgc taatgctttt atgtcgaatg ttatgactct tgtatctaat gacagttatc
    13801 ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt aactgctgca acattaaatc
    13861 ttgggctaga taagaattta ggttatgcat atttagttcc attcaatagt aaaaacaagc
    13921 aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg ctataaagga tatatccaat
    13981 tagcccaacg atcaggtaaa tacaaagcat taaatgtgat cgaagtttac gaaggagaac
    14041 taaaaagctg gaaccgactg acagaagagt ttgagtttga tccaaatggt agaacgtctg
    14101 atgaagtcat tggatatgtt ggctatttcg agttactgaa tggattcaaa aaaactgtct
    14161 attggaccaa acaagaaatc gaagctcatc ggattgctaa caataaagat cgcgataaga
    14221 caaagttaag tggtgtgtgg gcatctgatt acaatgcaat ggcacgaaaa actgttttga
    14281 gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat gcaagaagcc accacatcgg
    14341 atgagagagt ccaaagggtt caagaagacg gcagcattat tgctgaaaca gaagttgaag
    14401 aagatattcc tgaaagaaaa gaagcagagg ttatatctga agaaaatgaa gatgtacaaa
    14461 ctggattatt tgatgcatct aatccgccgt taaacaaata atgagggagc tttctccctt
    14521 gcttttctag aggagaaata cgaatgaaca caggatatgt caaattacat cgaaaagtga
    14581 tgaattcatt cgtttggacc aatccttata tgtataaatt atggagcttg tgtttgatga
    14641 aagcgagtca tgagaatcgc aagatacttt ttaatggaaa agagatacaa gtgaacagcg
    14701 gagaattcgt cacagggcgc gatgctatca catctgagat gaacgaaggc gtgaaacgtg
    14761 aacatcaagt gaacagcggt tctgtatgga gatggctaaa acaatttgaa aaaaacggaa
    14821 tgttgaacat caaatcaact acgaaataca gcgttatatc aataaaaaac tggtctttgt
    14881 accaaagcag tgaacaacaa atgaacatca aacgcacaac aagtgaacaa caagtgcaca
    14941 caaacaagaa tgaaaagaat tataagaatg aaaagaataa taaaacatca tcgtcgcaac
    15001 cacgcaaaaa gcgtgtttac gacaccgact cagtttacta cattctcgcg gaggagttat
    15061 tcaaacagat ttgtcagaat caggaaatca aaaagccgaa tctgcaaagt tgggcagata
    15121 acattcggaa aatgatcgag atcgacaagc ggaccgaaaa ccaggtacgg ggaatgattg
    15181 aatggagtca gcacaatgtg ttttgggcat cgaatatttt atctgctaaa aaattgcgag
    15241 aaaaatacga cacaatggca gcgcaagcaa atcgtgatta taaaacaaaa caaactaaaa
    15301 cgcttgaata cgagaaattt agcacagatg agttgcctat ttgagaggag gcgtaggcat
    15361 ggaaactgtt ggggaaatca tggaaaagct gatacagaaa gtgcttgtcc aacgtggcga
    15421 atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg aagaaccctg atggctcaga
    15481 acgttgtaag ccaacctgta tgcaatgtgg gtataaagca ttgcgagttc aggaagactt
    15541 acaaacagaa cgaatttaca acgagagcct gaaagcaaga gcaatcaatt ttttcaaagg
    15601 tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca ttgcagaatt atcaaattgt
    15661 cgatcaagaa acaagacaag cggttgaagt aaccaaacgc tttgttaatt cagtcttgtt
    15721 aggaaatcca agtcaccttg ttttaactgg aaaacaagga acaggtaaaa gtcacctagc
    15781 gatggcagcg gcttgggaag tcttgaagcg atcaaactac gataaaaaaa tcttatttat
    15841 cgggttacag gaaatgctgg atcaaatcaa attttcgtac aacaatcctg aactcagaaa
    15901 aacgattgag ggatcgttga ttgcagatat caaaacagcg gatttagtca tcattgatga
    15961 tatcggttca gaactaggaa aagatgcatc agatagtcga gcgtttggca taaacacgct
    16021 aaattcgttc ttggatgcac gacagaacct agcaacgatt atcacaacga acttgcttgg
    16081 tgaagaactg aaaaaagctt acggtacgag aacaatatca agaatgtttg tcaactctga
    16141 tggatttacg atggtatttt ctcaaacagc agacaagcgc ataaaaccag tgaaaggtag
    16201 tatcgcatga ataaataccg taataaaaaa actgttcatc gaggtatcaa gtttgattct
    16261 atcgcggaag cagagtatta cgatctagcc ttgtggcaag ctgaagcgaa tggttggaaa
    16321 gtgaagctcc aggaaaggtt tgagctgatg ccgaagtttg aactagaggg caagaagtat
    16381 cgcaagatcg agtatattcc cgacttcaca ttttacaaaa gcggcaagct ggtcaaagtc
    16441 gtagatgtta aggggatgca gacaaaagac tttaagatca aggcaaagtt gttctgtcat
    16501 caatatcaag tgccgttgat attagccaaa aaatatcgga atacgttcaa ggaagagcgt
    16561 ttttaacgag gtggtccatc atgacaacag aagaagtgat tcaaatgcga attcgaagcc
    16621 ttcggcgtga gattgacgat ctggaacgaa caaaggcagt gatggtcaat gaaacggcta
    16681 gaaaggcaat cgatttgcac atagagaatt taagaaggga aatccgtaga ttggaggaat
    16741 gagcgtggat aaggaagcgg cttggcgaaa attaatgttg ctgattcaag atgagaactg
    16801 gcaagaagat gaagcagtgg ttgctgaagt tcagcgtcta ggaaagattg ctaacggacg
    16861 tatacgaaaa aagccagaca aaagaaaaca gcgcaaaggg aaaatcgtcg ttgttttaca
    16921 cgaaggcaaa attttgatgc aaggaacagc tagtgagctg tctgcagaaa ctggatatac
    16981 gcgtgggact attcgaacgt acgcttggcg aaatcacacc gatcgaaaag ggcacgaata
    17041 taagtatttg gaggaagaaa aatgaacgaa aacaaattaa tcaaattggg tgtagcagga
    17101 gcagtaatcg taggtattgg agttatcgga ggatttaagt tcttcgaaaa aatcgataat
    17161 ggatatgtgg gtgtgcgcta ttcaatgaac ggcggtatca aagatgaagc actgacgcaa
    17221 ggtgtgaaat ttgtagggat tgacaaagtg atccaatatc caattcgctt gcaaactatc
    17281 caatcaaaaa atatttcagt atctacaagc gacggcaaaa agacaacgat tgatatcaaa
    17341 tatgactaca aagttgattc aactaaagca gcaaaaatgt acaaggaatt tgggaatatc
    17401 acttcggaag atatcgaaag tggatggtta aaatctaagc ttcaaaaggt cgctcgtgaa
    17461 gtttatgcga aatatagtct gcttgatgtc ctttcaggag attcctctaa agttgaagct
    17521 gaggtattaa cgaactttgc taaatcagtt gaatctaaag ggtttgaagt cgaagacgta
    17581 acacttggtg ttccagatgt cgataaagaa acacaaaaat caatcgatgc gatcattcga
    17641 gctggtcaag aaaatgaaaa agcgaagcta gacgcagaaa ctgcaaaaac tcaagctgat
    17701 agtgaagctt acaagaaaac aaaagctgca gaagcagagg cagaatctaa tcgcaaagtc
    17761 gccgaatcag taacagacaa tttgattcgt tatgaagaag ctcaagctcg caaaaagcat
    17821 ggatgggtaa cagtaaatgg agcagatact gtagttacgg atgaagcagg caaataatat
    17881 gggattcttt atggctaaaa ttctcttgtt cttaggttta gtaggagcag catatctcgt
    17941 gtatgcgctc ctttcccaaa ctgatgacaa agaagatgac aacaacgatg aaatgaaatt
    18001 ttaggaggag aaataatgga cgaactaatc acaaaagtag agcagtgggc taaagacaag
    18061 ggactggatc aagcagatcc aaaagcacag tttttgaaag tagctgagga attcggggaa
    18121 atagcttcgg cgatggcaag aagtaatgat gaactattta aagatagcgt aggagacgtt
    18181 atcgtcacgc tgattatcct ttccatgcaa aaagggacaa acgtacaaga gtgtttagaa
    18241 atggcataca acgaaatcaa aggacgcaca gggaaaatgg tagatggtgt attcgtgaag
    18301 tcgagtgatt tggaggatgt gaaatgaaat acgaaatacc actaagtgaa gcgggcattc
    18361 aagcaattat caatggtcgg gaggttaaca tagaacttcc tgatggtact gaattagtca
    18421 tcagacaaag ttatttgaaa gatatggcag ctccagtatt aattgatcgt tttaacgtga
    18481 ctgattctgt ggtagagaac cacttaaaag aatttcgatc aagtatagac aacactttca
    18541 gattagggag ttgattgaca atgaacacca gacaccgcag agtagcaaaa ctaagaaaac
    18601 aggaactaaa tgtactaaag acaaagtttg aaaaagaata tggaatttca gcagaagaaa
    18661 catataaagt ggcaagtcag tttgttgctg atgcaagtga tgctattcgt aagtttggga
    18721 tttcgatatt aaatgatgat cgtaaatggg aggaaatgag atgaaactaa aagacggatt
    18781 ttacgctagc agtcacggta tcggcggttt aatgctagat atgccgacaa agaaccctaa
    18841 aacacgcaag aaatcaaaat tcaaagtcgg tgacatggtc cgctgtgaag cagaagagtt
    18901 cgtttatccg ttcagaggat acgttaaaaa gatactgtca aactcagcaa tcattcgtat
    18961 tgaaaataca atgaagtgtg atcgttcgac tgcaaaacac aagcattatt tagctgtagc
    19021 gagattagtt gacatggaag taatcaagag caaataaaaa aagccggatc gctccgactg
    19081 attcaataaa tccaacacat ttattatatc acataaagga gcggtttgac ttgatgcaat
    19141 tgttacgaga ggtagatttc aaacagacaa gatgtaatgc gagagatgtg ctgaagaact
    19201 ttcggcgttt ggagcggatg gcaggtcgct ctttgataga tattaagtcg ccgattataa
    19261 cggatatgcc gaaggcaccg aagcatggca ataaggcaga agacgcgatt attcagatga
    19321 tggatataga aacggagaga gatgcgattc tagcggcttt gatggctctt agtctgatta
    19381 gtcgtcagat actctactac agcttctgtg tgccagatag cttctcaaac tacaggatta
    19441 gccgtgaagt gggttattca gaaagaagca tacaaaggat gaagtcggaa gctctaatag
    19501 agtttgcaga agcgtataaa cacggaagaa taattgctta taaatagggg gaccatataa
    19561 tgtggaaaga ttatgtatcg ttgaaagagt tgaaaaaaga tcttgttttc aaaagaattg
    19621 tagaatggtc agagagtgaa ttaattttag aagatggaac caaaatggaa gttgtatgta
    19681 gcgaatcaga ttgctgcgca tgggccgaag gtgaattcaa aaatgtaaag cttgatgcag
    19741 tgattacaga tataaagatt tttgataaag gtaaccgtct ctataatggt gacggacata
    19801 cctcttatgc tgaagtcgtt gtttatcata atcgtaatga gatcgctaaa gcagaatgca
    19861 cagcgaatga tgggaatggc ggctactatt atagtgtttg tgctttaaag gtcaaagaca
    19921 aactttgtat agtgactgac gcataaaaaa atggcggttt tttggcggaa tgatggcggt
    19981 ttttagccat ttatcagtga tattatggta gtgtcgaaag attagtgata ggtctgagac
    20041 aaaataataa taaaaggaac atcgttttat tattgtttca caattaagct tcgatagaca
    20101 gcaacggaaa tattaagaat aaggatgtga attttaactc cttctaaatt gttcttatta
    20161 tctatcatcc gttgctgtct attgttacat acagatcgct taggcggtct ttttattttg
    20221 gccgaaaacc tacattttcg atggccgatt attggaggag gaatagcatg ttcaaactat
    20281 ctgaaatcat taagaagtcc gacgttgaga aattagagat gttaaaaaag aagttgaaga
    20341 aatagcagtc tcttcgtgag gctgttttat tttgctcaca aaaatagacc actatcgggt
    20401 aatagtggtc aggaattaaa tgaaaaagat gttagggttg ttagctaagt ataacatcat
    20461 aacgcttaca aaacaatgca agaaggataa attattatga gaacttactg gtatgtgtca
    20521 ttaaacaata aatatccgaa gccaatgaaa ggacagcata ggcgtgtagt catgtctgtt
    20581 caaatgaagg cgaagtattc gattgtagaa atgatcagag aggcaacgcc agtagagatt
    20641 gattattgca agctagtcta ttgtgggtgc ggccgttgga aagaggatca tgtacaaaaa
    20701 aatatcagca agtacatata gagaggcggt gattaaggat ggaaaaaagc gatacgaaag
    20761 cgaacagata caaacctact gctgcagaga aaaaactctt ggaagtattg ataaatccag
    20821 ataacttagg caaaccggtg caagagttgt gttcactggc gaaaataggt aggaccaaat
    20881 attacgacgc catgagcaaa gatggctttg ttgatctagt taacgaaaca acaatggatc
    20941 tgatcaaagg aaaagcttct gatgtcctta atgccactta caagtatgca ttgactgaaa
    21001 aaggtcatca ggataggaaa atgattctga ctattgctgg aatctatgca gacaaacaag
    21061 aactcaaaca ttctggcggc gtcgatatcc gaaaacagta tgagaaaatg tccgatgaag
    21121 aattagaaga gttggtgaaa cggtatgaaa aaatcaacga ctcttaatcg tgttgagtat
    21181 gccgttgttc aaagagagct agccattcga aaagctagga aaagtttttg gctattctgc
    21241 cagcttcttt atccagagtt ttacaaagat gatcggttgt atttgaaaga tttatgtcat
    21301 acgctacaag atttttacga agataaaatc gacaaaaaaa ttttggttat taacatgccg
    21361 cctcgacatg ggaagacttt tacagcgcgg ctattcgttt tatggatgtt tggacaaaat
    21421 ccaaagacga aaattattac cggatcttat aaccaaatcc tgtcatcttt atttgcgcaa
    21481 caaacacgtg atggaatcat ggttgaaaac gaaaatgtta aggctgaata ctttaaagat
    21541 atttttccag aggtgcaaat caaatatgga gatgctgcaa agggtttctg gtctttagat
    21601 ggttcagaag aaaagaatta tttagcgact tctcctggtg gaacatccac tggtattggc
    21661 gcgaattatg taattgttga tgacatcatt aaaaccgcag aagaagccta taacgagcgt
    21721 gttttagatt ctcactggga atggtataac aacacgttgg cccaacgaat ggaacggcca
    21781 cgtaaacaga tattgattat gacacgttgg tcaagtaatg atttagccgg aaaaatgttg
    21841 acgagacgaa aaaataatgt tcatcagatc tgttataaag cagtacaaga agacggctcg
    21901 atgttgtgca acgagattat gacccacgat gagtacttag atgtggtaca agaaatgaat
    21961 gtagatattg cagaagccaa ctatcagcaa gaaccaattg accaaaaagg caggctatat
    22021 cagaagtttc ttacttatga tacgttgcct gataacatta tcaaaatatg gaactacacc
    22081 gacaccgccg acaagggtgc cgattacttt gcgtctcctg tttttgctga gacgtctgat
    22141 catgatgctt atattatcga tgtcatgtat acaaaagagc cgatggaaac aactgaaata
    22201 gcccacgcta atatgattat tcgcaatcga gttaataagg tacggattga aggaaacaat
    22261 ggcgggcgtg gattcagaag aaattcagaa agaattacaa aagagcgagg ctatcgaggt
    22321 gcagtatttg aagactttca tcaatcggat aataaactgt ctagaatact ggcgaatagt
    22381 gcttgggtgg aaaatcatgt ttactatcca gatgattggg cgacacgttg gccggacttt
    22441 tatgaggcta tggttagtta tcaacgtgaa ggtaagaacg ctcatgatga tgctccggat
    22501 gcagttacag gaattgcaga aacgattata gaagcagatg acttcgagtt tattgcttat
    22561 taggaggtgg gtctttgttt tattctggaa tcacattgaa aaaatacaaa cgtttaagaa
    22621 cgaaatattc aacgcaaatc gaagaaggat tattcgatcc taacgctttt atggaagata
    22681 tgaaaccctt tttcgatgat cgagaaagaa agtatcttgc ttatactagc gagaaaaacg
    22741 agatcgacag tcgtgagaaa ccaaaaacaa aaatcattaa ggtcaataac aagattcatg
    22801 cgggaatgta ttcagtggtc gttgatcaag cagttaacca tttcacagga ataccaatta
    22861 aatgggatta tgatgtttcg gaacagaaaa ggacattcat tcaacgaatg agagacaaat
    22921 tactgggtaa tgatatcagc gccccttcta ccccagaaga atttgaaaca ctcacgagac
    22981 aattaagtac tatgcggttt ggaatgttag accccgatac tgcaacattt caaggagcga
    23041 cagggcttgc tttcagatta ctggagcctg ttgagacaaa tgatggttgg gaactaagag
    23101 caagcaacat tgagccgtgg aaagctgaaa agtacgaaaa cggcgcagtg tacattaaag
    23161 agaaatacga tccatatcaa aagaagtttt atgaagagat gaaggttgtc acgccgaaat
    23221 caatatttac ttatgctcga tatactgatt accctggcac ggtacaagga gcatttaaaa
    23281 cgctgggtaa tgttcctaat cctttaggcg ttatcaatct tacggagttt aaaaacaata
    23341 caaaccgtta ctgtgacttt gaagttgcag aggagattag tgatgctatt gatcgtgttt
    23401 tatctgatca gcagaacgag attgaacaat tcaagttagc ttatatgctg atcactgggg
    23461 cagatttgtc cgaggataaa gccaagaaga tgatggagca actagggatc atcaatctga
    23521 aagaccctaa tgcgaaagcc ggctacgtga caaaggatct acaaaaggat ttcaacgagt
    23581 atcattttga tcttcttaaa aagcaattct acacggtgtg taaagcaatt gatttcaatg
    23641 acgaagtctt taagtcgaat agttctgggg aagcccgaaa gtggcaaatc attagtttag
    23701 aagctaagac aaataccaaa gaacagtact ttcgagaagg attgaaagag tgtgccgagg
    23761 ttatggctgc ttatttggat tttgccgaga aagttacaat tgatccggaa aagatcattt
    23821 tcaccttctc tcgttcgctt ccaacagatt taagctacct agctgaggca ttgcctaagt
    23881 tggcaccata cgtttctaag aggacgattc aaagtcagat cccgttcgtc acagatgttg
    23941 attatgaaaa cgaaatgatt gacgtcgaaa gcagacaatc ttacccagac ggcgagtatg
    24001 gaggtggcat cgttgagtca aatagaaacg aagtactgga ccaaacgtcg agaactggaa
    24061 gatttagcaa gactcaaaat ggaaaatcaa acgctaaaga aactaagtag tgtctttcct
    24121 gatgcgttga aagaaattca agcggagtta ttatcccaag ctgatttaca cgatattacc
    24181 tatgctgaga tgatggaatt ttatagtaca agtgatcaga aaaaatatcg tgaatacgtt
    24241 gaaaagaact acaaatcact gaagatgtat gatgcaaaat acaaagagtt catcgaagaa
    24301 tactttcctt ctttcgacta cgccaaagtg aatcggctgc ttcaaatgag gtcagatatt
    24361 tttcgaattc ttgcagagag tgcaatagat gcagatgtga ataaatattt ttcggatcgg
    24421 ttagaagata tcttgcaacg ttcgtatgta tcaaacgcaa acgtatttgc ccagattttg
    24481 aacgtagatt tgccgaatta cttatcaaag gaagaacttg aacgttacct gaactatcct
    24541 tggtcaggta agacattctc gcgtcgttta tggggcaata tttcatctct agaacaaaag
    24601 ctatctaacg ccattacaac tagtgtagca agtggcgaag gagttctcgt ggcattacga
    24661 acaatgcgag aagacccaga aatatgcgat atgttcaaaa gggaggaatc gaagtacaac
    24721 aaggcgatcg aaaatcttgt gagaacagaa tacgctaaat tcgctcaaga tggtattgag
    24781 aaatcttata ttgaaactgg cgtagacgaa tacaatgtct taactgcaaa agatgaaaag
    24841 gtatgttcaa tatgcggtgg caaagcaaag aacaacccat acaagttgag tgaagcgatc
    24901 attggtgaga accgtgcgcc ttttcatggc cgatgcagat gtacagatgt tcctaatatg
    24961 ccgaaattag gaaaagatat tgacgaagag tatgagcgtt tatttggcga tttattagat
    25021 gagtttgcac atgattcttt tggaattaat ttgaaacgga ggaagtagaa tgaaagattt
    25081 ttttgaagca gtactaacaa ttaatgtaaa tgctgatatt gcagaagcct acaaaacagc
    25141 tattgagtct gagaaccatc ctaatggctt gagagaccat tggaatggca attatgccta
    25201 cgtggttatt ggcgatcaaa ctgttaatta tcaagataat actccagttg ataagaatac
    25261 cgttaattta acgattcaat tattatctca ttcattacca aatttaaaag aaacagttga
    25321 ttggtatgaa aatatgggat gtattgttgt tagaactgac tacaaagaag gaaagtctag
    25381 taattaggct tttttatttt gtccgaaatg acgttaaact agcgcaatac tgggcttggt
    25441 tgaatggtgg ggcgcaacta ttaaaactca aagcaatgcg gggcgtgaaa acgaatcgtg
    25501 gggcgaaagg agaatgatta tgaaacacaa atcattaatg ccaatgaatt tgcaatactt
    25561 tgccgaaggt gacgatcaaa agttttcttt tgatgacttc aaatcttttg tggaatccaa
    25621 tgaagaagct caaaaattta ttcagtcaca atctcaaagt gttgccgaca aacaattgga
    25681 agcttggaaa caaaataact tagataaaat taaacaggat accatcaagg aatatgagga
    25741 atctaagaaa aacaagtcac ctgagcaaat tcaactggaa aaactacaag ctgaatttga
    25801 agcagaaaaa gcgttgcgtg tgacaagtga taataaagca tttgttgcag aacaaattgc
    25861 tggattagaa ctagatggag agttaaaaga gtctatttct caatttatgc taaataatct
    25921 tgttagttcg gatacagatt tcactaagaa tgctgttgaa ggttttacaa gtgttttgaa
    25981 tgcaattaat gagaaacatg cagatgcatt aaaagaacta caaatgaagt ctgcatttgg
    26041 tggaactcaa caatcgaata accaggttca gcagaacaat gaaacattaa caaatccaga
    26101 agaacaatta ggacaaattc ttcaacaatt taactaggag agtgaaaaat tatgaaaaaa
    26161 acatctttaa ataatttaga gtatttggat atttcaccag cgattaatgc tatgcaagta
    26221 ccaaatacac cttttttaag ctatttactt ggtgctggaa aaacagagcc agcaaactcg
    26281 acagaaatta aatggcgtga atatgatatc aacaacgatg attcttctga aaaacttgag
    26341 ggcggagaat atccagatgc tgaatcaggt cgaacttggt ttaacaacta tactgaaatt
    26401 tttagaaaat caacctctgt atctggtaca ttagatgcta ttaatgtgaa tggagtcgga
    26461 aatgaattaa ctaatcaagt agcactacgt ggtatggaaa tgaaaattga tttgaaccga
    26521 aaattgatta ctggtgtaaa agctgatgaa aatggttcta aaggtcgtcg aatgaatggg
    26581 attttgaact tgatcaattc agcaaataag gcagaaacag ccactgcggg tgcagtaaca
    26641 agaaaagata tcgatacttt atttaaattg atgtatgaaa aaggttatat gggagaaaaa
    26701 ctatgcttga tttctccaga tatgcaggag ttaatgactg atgagttaga tggaaaatca
    26761 acaaaaattg ttcagttcgg ggaaagagta acttttggat tgcaaattgg aaatatcgtg
    26821 tctaattacg gtacaggtat tgctctacta gaaccatcat tgccaaaagg aacaattgcc
    26881 gcaatcgata ctaattatgt gaaactacgt ccattacgtg aatggagagc agaagaactt
    26941 gcaaaaacaa cggattctag acgtatcggt cttgtaggtg aatactctct tgaatacaat
    27001 gcttcaaact ctggggcaat tttaaattta aagtctgaat aaaagggagt tagtactccc
    27061 ttttttgata ggaggaatta cagtggtaaa aaaagatgaa actaaaaaag atgaagtcgt
    27121 gaaatataga gtaggtaaaa ctaaaaattt tgttgggttt gttcatccta aaactcgtag
    27181 atttatcaca gcagattcaa ataacgaatt tatcatttct atagatgata aagaagcaat
    27241 tgcaattttg gaagatgcaa ttgatgttaa tgaaatttag gaagtgatct gatggatgaa
    27301 tcgctaaaaa cggaaatcat tgagtctaca aaagaagatt ttccagattt gagtgaagaa
    27361 cgcataacta atttattaga aataattttg ctagaaattg aatcatacaa cacttgtaaa
    27421 aatgatattt catgggaaaa gttaaaaagc gtgattagtg aagtgttgta taaaataata
    27481 aaaaatgaat cagaaaaaac agtatcttca gttagacgtg gcgatacaac gattagttat
    27541 gcttcaacaa caaatgatgt tagtgagtta cttctaggct atggtgattt gatacgaaga
    27601 gttattggtt gtggaggatt ggagtttttt taatgaatga agcagatatt ttagaaacta
    27661 cttatgaaga tagttgtatt attgaaagac tgacggacat tgaagattct aatacaaata
    27721 ttactattca agattacaaa aaagtatatg ataatcctat ttcttgcgct ctttcacaag
    27781 gtcaaatcga tggactagca gtcatagaag atggagagat ggtaaatgtt tcaactgaca
    27841 catataaatt atttgttcat cctaagatta aactaaaaaa aggagatcga ataacaataa
    27901 ctcaaaaggc cagtggctta attttttctc tatttgctac taagcctttt tactatccta
    27961 gtcattgtga agtgaatttg ataggaagtg agaaaaatgg gtaatctcaa atatgaatca
    28021 aatgcagaaa agatcattga gaattttaaa aatatgactg taattgctca aaaagaagga
    28081 atatccttcg ttaacgattc aatgaataaa gtcgttagtt tagctaaacc attaactcct
    28141 gtaaaatcag gtaatttaag gcgaggatat cgtgtagtaa aagctagaaa actatcaagc
    28201 ggtcgaattg ttggagcagt tataaacaat gaacattatt ttaaatatgt agaagaaggt
    28261 cataggacta aaaatggtgg ctttgtcaaa gggaaattca tgttaactcg tgcaacgaat
    28321 cttgcaaata tgtcttatat tcctcgaaga tttaaacaaa tggcaataaa aatcgttaag
    28381 aaaggaaagt aacatgaaag atgaaatcat tgctgcaatc agtagcaaat taaaagaaat
    28441 ctatcctgat gggacaatat atcttgattc agttatgcag tcaactaaag atttttattt
    28501 cgttttatca gtaatggaat ctggaactga aaatgtagga attgatattc aaaatgtttc
    28561 tttcttaatt gatattgcat tgattgataa taaacctaat agaaatttaa ttaatgaatt
    28621 agtctcacgt tgtgggactt tttttaatac gattacaatc gatgagcaaa tattatttcc
    28681 aaaagcctat ttacctgatg aagcagatgg tgttcaacat attcgtttta cattagaatt
    28741 tccacaatat attgaatgga gtgaaagata aatgggagaa aaaagaagta aaaccggaat
    28801 tatttctgtc gaaaaaccta cttggtttcc tttggaagat gagacaggga atttcccggt
    28861 atatggtact gcgttgccaa tgggaacagc agtaagtatc aaaccaacag ctaattatga
    28921 aacaacacaa gattatgggg attcagtggt tcaagatcaa tttacagctt ttggtggtgc
    28981 agaagttgaa ctggaagcaa atggatatac tcataaagtt ttatctacaa ttactggagg
    29041 gaaaattgtt aaaggtggtg ccttgcgttc tggagaagat attgcgcaag atggtgcgtt
    29101 tgcatataga cgtaaaaaat cgaatgggaa ataccgttat acggtctttt ataaaggaca
    29161 gtttgctcta gattcagatg aaacgtcaac gattgaagga agtaaagtaa gttttactca
    29221 tccaacttgg aagggttcat ttgtagatgt accaggactt ggatatatgt attcagttga
    29281 tgaagatgat gaaggcgtgg ataaagcaat gatcggaaat tggtttacaa aagtagcaat
    29341 tccaattgaa gaagcagaac tttcaggagg tacagaataa tgtcaaaata tcaaacaacg
    29401 attaaattaa cgaaaaaaga tgaagaaggt aaatatgagc aagtacaatt taaatctgct
    29461 gaatttttac caggaacagt tgtagaagat gctgcaggag ttatggaaga aatgcaaaca
    29521 gcgactgata aacaatcagt taaaaaagct ttaagtcgtg cttattcatt tattgcagat
    29581 actttatttg agggacaatt tactggtgaa gattattgca aaggaattga tgctcgtgag
    29641 attgcctcat tgacaggaaa attattgaag tctgttactg caggttttga tgaaacttat
    29701 acagagacga aaaaaaagta agtgaggcgc tcaagtcacc ttcatttaag tattcgatta
    29761 cttaccgaga attagatata aagacgcaat tactcgaagc aggttggacg ttaccagaaa
    29821 ttgaacgtac tgacttggat gagttaatgc gtctttttgc ttttagagat gcggtaaaag
    29881 agcatgaaga tgttgagtac tatgataatt tcactcaatt ttaggaggtg atactttgaa
    29941 taacgatgac ctaattctga agatgatact agatgaatct ggttttactg ccggtatgaa
    30001 taatgctgtc aaaaagttga actcttttga tgaaacaatc gaaagaaaaa gtagaaatag
    30061 tggcagttca ttaggtagta tttggaaaat atttgctgga agttttctag ctagcggagt
    30121 aactagaatc gtaggagctg gctttgatct aatcaagggt tccataagtg gagcggttga
    30181 tcgagtagat acgatgaata acgctctacg aaatttccaa aatatgggat ttagtaattc
    30241 agaaattatg aagaatatcg gaaagaatgg gcttttatct caaggtattc aaggacttcc
    30301 tactgctttg aatgatgcga taagtcatgt tcaacttctt gcttcttcta caggtgattt
    30361 aactcgttca actcaaatat ttaaagcttt aaatgatgga attcttggtt tcggtggttc
    30421 aactgatcaa gttaatgaag ctgttattca attatctcaa agtttctcaa acggaaaagt
    30481 agacgcacaa acttggaact caatgatcaa cgctcaactt ggacctactc tttctgctat
    30541 tgctaaaaag atgggaatta caatgggaga tctgaaagaa ggtttgtctc aaggtaagat
    30601 ttctgttgaa gaattccaaa atcaattaat agaaatggat accaaaggtg gcggaggact
    30661 taaatcatta agtcaaatcg ctaaagattc tactaaggga attaaaacct ctatacaaaa
    30721 tgcaaaaaca gctgttacac gtggtgttgg tgaggtaata gaaggattaa ataaagcatt
    30781 agtagattca gatttaggtg gatttaaggg aattattgat aaagtgggca gttcaatgga
    30841 atcgttctta aaagtaattg ctgcaaatat tcctacagca gtatcatttt taagtaattt
    30901 atttaacgaa attcaaaaat ttggttctgc tttgaaattc atgatgccct ttcttgttcc
    30961 tgcagctacc gcttttggag cgttcatgtt tcaacttaaa ggtataccag caataataaa
    31021 aagcttcaat aattttaaga atgccataat tggcgttgga aattcattaa agattatggg
    31081 ggcaatagct gctgctaatt catttgtttt aattattggt gcagtcgttg gtgcaatagc
    31141 tgttttcggt tactttatgg caaccaatga agagtttaga aacaaggtta tatctatttg
    31201 gaatgatgtg aaagattccg taattggtgt attaaaaaat ataaaggact ggggaattga
    31261 tacttggaat tctgctaaag aaatggcatc aaatgcagtt gagggtgtca aagatgcttg
    31321 gtcaggaaca aaggaatggt tttctaacac ctggaaaggt attaaggatg gggccacagg
    31381 tttatttgat aaaacagtag agacttcaag aaatgcagtc gatagtgtaa aaaatgcatg
    31441 gtctaatatg aagaaatggt tttctgatac ttggcaaagt ataaaagatt cagccagaga
    31501 aaaatggaat gaaattaaag gttctattat gtcggttgct ggtccattga ttacaggtat
    31561 aaagaatgca tttttacatg ttacttttta tctagagacc ttatggaata atttagttga
    31621 gattggtaaa aacgtctttg agattttaaa aaatgttata cttgcgccgg ttttgtttat
    31681 tacctccatg atttctggtg gatgggaaga aacaaaaaat aatatgattg ctgtttggaa
    31741 taatattaaa gaaagtgcca taaatatttg ggaatctata aagaatatat ttgtaagtta
    31801 ttttacaaat atttattttg ctgcactcaa tatttggaca gggttcaagc ttacattgat
    31861 aaatatttgg aatgaagtgg taaatcaagc taaatcgatt tggttaaaca taaagtattt
    31921 ttttattaat ctttggattg atataaaata ttttgcaatt cagaaatgga ttgaattgaa
    31981 gttttctatt attcaaactt ggatcgattt aaaatataat gccattacta cttggaataa
    32041 tattaaacag tttttcaaag atacttggaa aaatattaaa gatacggcat acaatacgtg
    32101 gatttctata aaaaatacca tgattaatac atggaataac attaaggact ctttctggaa
    32161 tattgttact ggaattgtta attctgctga aaatgcatgg acaaatctaa aaaatggtgt
    32221 ttcaaatgct attaatcggg taaaagaaat ctttaattcg ttaagggaag tcaatttatt
    32281 tgaaattggc aagaacatca ttgatggact tataaatggt gtaaaagaaa agtggaatgc
    32341 tttgaaagat actatcaaag gtattgcagg taatatcaaa aattggatta aaggagcttt
    32401 agatattcat tctccatcaa gatggatgcg tgatatggtt ggtaaaaata ttgttcaagg
    32461 tattatcgtt ggtattgata aagagcaaag taagttagat caaaccatga caaatttagt
    32521 aaaaacaccg tctgttcaac ctgtagtaaa tggaataagt cacctaccaa ttacacaaac
    32581 aaagcaaaat atatctgcta gtgaaacaaa agaaattcat ttacatttaa atgtttatgg
    32641 agatttacct gattcaatgg ttcggaagat taccacaaaa attaaaacag aattgacaag
    32701 acaaatgaaa cgagatgctg gtgcagtagg aggaacatta tatgcaactt aagagaggac
    32761 aattttttat taatcaacat tattcttctg aatttaatgt gtatattcaa aacaggcctg
    32821 cttctgtttc agctagtcga gtaattgaat tgagagagcg agaaggaaat gattctatca
    32881 ttattgataa aggctattac agaaatgtga ctagaaagat tgagtgttac tataaagcac
    32941 cttcaattga tgtggtgcag gagtgggaag atcgaatcac tgaatggtta gatatgtcgt
    33001 cttatagtga ttttattctt tactatgatg aacaatatat ttatcaagct gtagtaatag
    33061 aaggacctga atttaaagga acaagaaaaa caggaaatat tgtccctttt gaatttacag
    33121 tgagcattcg accatttaag gaaaattaca atggtagatt tactattcga caaacagaaa
    33181 cttttgaaat ttataaccca gagaagtatt cttcaaaacc gcttattaaa ttgagtggtt
    33241 ctggagatgc ttctttttat attaataagg acaaatatga tttgaaatcg ttggacagag
    33301 aattatttat agattctaaa ttagaagagg cttaccgaaa attagatggt aatttagaac
    33361 atcaggatca agtcacttta tttttagatt ttccattttt atatcctgga aaaaatgaaa
    33421 ttaaatggac gaaaaatatt cattcatttg aaataatgcc taggtggtgg agaaaagtat
    33481 gaaaccaaga atatatagtc ctactgaaac agatttctca acgaatggct taggaatttt
    33541 aaaggattgt acaagatgtg aaatatatga agtagctaat ggaaaatatg aattagagtt
    33601 ggattatcct ttaggaacta gatttgatga atattttgaa aatgactatc aaataaaagc
    33661 aaagccaaac gatcaagaag agtatcatat cttttttatt gatgataaag atatagatac
    33721 ttttttaaat acagtaacta tttatgctca gagtcgtaca aatcgacttg gaagacgggt
    33781 agtcactctt gcggaagtag actctaaaac tggtcaagaa gcgatatcaa ttattgaaac
    33841 caaaatggat aaaaaatctg acatacgact ttattctgat attacggccg tttctagcac
    33901 aatctttgaa gcgagaaatg ttttaaattg tattgctggt gaacaaggat cattgcttca
    33961 atattggggt ggagaaatta aacgtgaacc atttaagctt tctttgttaa agcgaagagg
    34021 tcgcaataat gttggaacga ttcggtatgg aaaagatatg tctggtttaa aggtcaaatt
    34081 agattggaca ggtataaaaa caagacttat tccatatgct gatcctcaaa gtgaggtagg
    34141 tacgactaat cgaatttatg gttcgccagt agatagtgcc tatattaata actatcctga
    34201 tgtgtataca gagcatgttc agtttacaga agaacaagga gtaaaagaca ttaagagctt
    34261 aaataaaata gctaaaaatt actttaaaac gatcaatcct ggctgtgata agcctaaagt
    34321 ttctattacg gttgaatttg ataagttgac tgatagtgaa gaagcgaaag aatttgcgaa
    34381 gattagaaat tatggtctgt ttgatacatt taaaatatat cataaaaaat ataatattta
    34441 tcttgaatca aaggttagtg gactacaata cgattcttta tctgaaaaaa ctttgaaatt
    34501 agaagcagga gacattcaag ttgcttttta tcagcaacaa gctgtaacta ttcaggataa
    34561 attaaaagat tatgcaacga ataattatat gagtgatttt aatgattatg tttcttcaat
    34621 gattacaggt caaggcagtg caggaggata tgtaagttta tggccaaaag aaaaaccttc
    34681 caacatcttt attatggata atccagattt agaaaaggca agagaagtac taagaatgaa
    34741 taaaaatggg attgcttttt ctaagaaagg atggcggggg ccttttactt cagcttggac
    34801 attagatagt atatttaatg ccaattttat acaaactgga ttaatcaaag cagatatctt
    34861 tcaaaattct tttaacaaga ctggagatgt attgaaatta gttaatggac tactccaaat
    34921 ttggaacaac aaaaagaaaa taatggagtt aactaaaaaa ggaatggaat tttggaattc
    34981 taagggttca attggaacga ttggaacaac tgattctgct ggtaatcctt ttcctggggc
    35041 ttctactcca acccctattg aagataattc tttagttatt cgtacaaatg gagacggcaa
    35101 atatattttg atttctccta aagtaggaaa aggattagtt ttattaggaa atggtaaagc
    35161 aatttatttt ggagacttag atgtacaagg aaaacttaca gttaaaggaa aagaaataat
    35221 tcctggacaa ggtggtggtc catcgggagg tggagaatct actggtgggt atcctagtga
    35281 attaaaaaca gatgcagaaa agagagcttg gagaatttac gatattttgt gtaataacgg
    35341 atttacgaaa caatctgcat gtggaatatt ggggaacatt caacaagaaa caggaggaac
    35401 ttttgatcct gatactgttc aaataggtgg accagcatac ggattagttc agtgggatgg
    35461 ttcttcatat cctcttgttg gtccagcaac atgggatgga aaagtttatg ttcaaaactt
    35521 atttaatgct gcaggtatta aagaaccaat aacgagctta gatgcacaag ttcgattgct
    35581 tatttggaca tttacaaatg gacaatggat gggcgtagta caacctacga cggttgatgg
    35641 atttaaggcc tgtactgatc caagacaagc agcatatgct tttgaacgaa actatgaacg
    35701 tccggcagcg acacatcctg aacgtcaaga ttatgcagtt aactggtata acaaatttaa
    35761 agatttaaaa ccaggaggag ctactggaga agcaggacta aaacatttag agtctttgat
    35821 tggacaacga attggcaatg gtcagtgtta tggcttgtct gcagaatatt caggatatct
    35881 gggtggttgt ggcatgggtg ctggaacaaa atatggttta actcatgtga taggaaatac
    35941 ttctgcagca tctgatattg gtattgccta tgattggtct gctgttggtt ggaaagtgat
    36001 tcaaaaccct agatatgatc aattagtagt tggtgcaatt attaattggg caagaggtgg
    36061 acaagtgggt tcatggtttg cagacggaac ttatggacat actggcgtta tccgaggctt
    36121 agctaatggt cgtatgcaaa cttatgaaca aaacacagag ttaggtatga tttgtggaaa
    36181 gttagatcgc cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata
    36241 gggaggtgat ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta
    36301 cgtggggatg attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat
    36361 tgtcgaaaat ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt
    36421 tggcggaaaa tatccagtcc aattagaaac aagaattgtg gatgctaaaa aagggaaaat
    36481 caaatatatt tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat
    36541 tatatttaaa ggagaagact tgattggaac aactcaagac ttctcctatt ttgtcatcaa
    36601 tgctgtttca aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat
    36661 tgctgatatg acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga
    36721 aaggaaagaa gaatttgatc gctggagaga agaacaggag aatagtttcc aagattggag
    36781 agaaggacaa gaatcagatt atttgtcatg gtttgaatca atcaaggata ttttgaagtc
    36841 tatcgatcca ggtggagtaa tgttagccga attaatggat gcacgtgttg atattcaagg
    36901 agttcgtcat gcgtctattt cagagcgctt gctggcagat atggattatc tatatcagaa
    36961 attacgagca acacttttca cgattgaata cggtgagatt gaagtgactg atattttgca
    37021 ggatgatctc ttttcagata atcacgaagt cgaaaaagtt ggaactgtag aatttccaat
    37081 tgaagaagga gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct
    37141 tgagaaagtt ggggtgattt gatggttaaa gtaaagagaa tgatggagac tgatgaatct
    37201 ggtgttaaac gtcaatttat gccaattaca catgtttctg cagttttagg cttagaaaaa
    37261 atgatatcag gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta
    37321 acgaaagctg atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca
    37381 agcgatggta ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca
    37441 tatattttgc cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta
    37501 gaagttactg aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa
    37561 gaattaaagg gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt
    37621 gcagaagatg gaacaattag ttctacaggt ggtagtggga ctggtggtgt taatcagtca
    37681 tatgtagatc aaaaattcca agaagctgta aatcaagcag aaagttacac aaacgaaaga
    37741 attccaaatt ttacttttga aaaaatcggg gaggtataga aaatgacaga tatcgtaaaa
    37801 ataaaacaaa gcggtgtaca ggtttatcca caaactcatt ggaatgctat agaaggcaaa
    37861 ccaacaacgg tgaagggaga taaaggcgat cctggccaag cagcaacaat taccatagga
    37921 accgtttcta gtggttcaac cgcttctgtt acgaatgtag gaacatcatc tgcagctaga
    37981 tttaattttg ttttaccaaa gggagataaa ggtgatcctg gaataaatgc cacaactaca
    38041 gctgtagcta caactacagc aaatgggttg atgtcgtcca cagataaaac taagttggat
    38101 ggaattgcag ctggagcaca gaaaaatcca ggtaatgcta caactacgac agcaggttta
    38161 atgtcagcaa ccgataaagt gaaactcgat ggattaacca atattacatt tgagaaagtg
    38221 gggacggttt aatggctgat attgtgcaat taaaagaaga cggagttgct aagtatttga
    38281 aaactcatgc caaaggaatt gatggtgtgg agggtgtttt agtaaaagcc acaggtaatg
    38341 aaacggtctt aggaacgaaa aattttaaag acggattgca attcaatgga ttgcctgtgc
    38401 aagcaggtat gatcgagcga gctatcacac tagctgatcg aagtgataca acaaacgtaa
    38461 ctgatgtgaa tggaaaaata atcagaatcg gaaacatcgt atttcttact tttaatttta
    38521 aatgtggcac atggcccgaa ggatcagaaa ctcgttggat tttaaaaatc ccggacggtt
    38581 tcaaacgtga tcaaggctat ccagcgcaaa ccgcgctttc actagttaga aatgcaagtc
    38641 agccagcgga tgcacgtgca ttcattgatc aaagtagcat catacaagcc aaatcaggtt
    38701 caggtagttc atatatttca ggtatgtgga tcactcaaga tccttggccg gcttaacaga
    38761 ttactaattg gaggaagagt aaaatgaaaa caatttataa agtattgtat ccaatgggat
    38821 atgaagaaca tcaagtagaa gataactttc caacatcttt accatttgtc gaagttccac
    38881 ctattttgtt tgaaaagaag gaagatgaaa cggatgaagg tttcggaaga agacaacagt
    38941 cgcaattctt taacttcact gaaaataagt gggaagaagc agttacacaa gattattcaa
    39001 aaaaattaga actactagaa aatttatcta taggtttaca ggtagataat gctgcattga
    39061 aaaaatcaaa tgaagagtta acggaaaaag cagattcgat ggcgcagttg aacgccaaac
    39121 tgatgcttaa tgatttaaca attaataaaa aaattgaagc aatagaaaaa caaattggag
    39181 gtgcttcata atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac
    39241 tgacgatcaa gttcgacaat tcgttccact atgcattaca gacgaagaag cagataaaat
    39301 cattagcaaa gaagagagcg catcttaatt gatgtgcttt ttattttgat tcaaggagtt
    39361 gtcacatgat taatttaggg gaatggggag cgatagcagg atcaataacc gctatcgttt
    39421 ctttgatttt attagtaata aaaccaatta ctgcatcttt ctcgaagatt actgagactc
    39481 tttcaaaagt aagtcacaat ttagatttgc tgactaaaga tttagaatcg agcaaatcag
    39541 atcgattgat gattcatgaa gaactaaaga aacacgatga aagattagat acacatgcag
    39601 aaaaattggt agaacacacg caacaaatta aaactttatt tagggagaga agaaaatgaa
    39661 taataaaacg ttcgaagtac taaaatggtt cgcactggta attattcccg cactagctac
    39721 tttcgtgggg ttagttggta aagcgctcaa ttggcagtac acagatatct gtgttgtcat
    39781 cattactggt tttggcgcgt ttttagggag tgtgttgggt gtatcaaatc gaacctacaa
    39841 aatgttctcg gctgaaagcg aagaaggagg aaacaaatga aaaagaaaat tactattact
    39901 gcgatgagcc tgttaacggc tcttttttta ttacccatta atacgtttgc ttatactatt
    39961 aatgacgagt ataatttagc gccgaatcaa ggagactcca gattagcaat tcctaacaaa
    40021 attattttgc acgaaactgg aatagatgca ccagcaagaa acgtagccgc caacatgaaa
    40081 aataattata acggaagcaa ttcttatact acagatgtta ttggtgacgg tgggattgtt
    40141 taccgtgtgg gtgagcaagg atatgtttcg tggggagctg gtaacgctaa tccttatgcg
    40201 cctgtacaga ttgaattaca gcgcacatat gataaagcat tgtttgaaaa aaactatcga
    40261 gcttatattg aatatacaag agatagtgca aaaaaatatg gaattccatt gactcttgat
    40321 caaggaactt ctttatttac aaaaggaatc atttctcatt tgtgggtgac aaattatgtt
    40381 tgggggaacc acacagatcc atatggttac ttatcgcaaa tgggagttag caaagaaaag
    40441 cttgcttatg atttagctca tggatttacc gatgaaaatc caactacttc agatgataaa
    40501 ccagtcattg atccaactag agcaggtgca gcaaatccta cgctgacaga tggaacaaat
    40561 cacgcccaca ttgatcagtt tggagaaatc gaaaacgcaa acttgcatgt ggatggatgg
    40621 cacattgcta attataaata cgagtatatt ttcattatgg attacaatac tggaaaagaa
    40681 ttagctcgag taagagctga tggaatttat agaccagatg taaatcaagc ttataatact
    40741 tctggaaacg ttggttatca tgtatctttc aatatgcgta attttcctaa taagaaagtc
    40801 tatgtaatga tgcgtgcaac gaatgatcca gaagggaaca ctaaaggcgg agcacaagat
    40861 tttcatgata aacgctggta tttaaatatt ccgcaacgat aaaaaaagcc cctcgat
//
LOCUS       DACEMS010000011        38176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEMS010000011 DACEMS010000000
VERSION     DACEMS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.011"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            308..1192
                     /locus_tag="IW735_000539"
     CDS             308..1192
                     /locus_tag="IW735_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1043562.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            1401..1979
                     /gene="pgsA"
                     /locus_tag="IW735_000540"
     CDS             1401..1979
                     /gene="pgsA"
                     /locus_tag="IW735_000540"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1043563.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2219..3460
                     /locus_tag="IW735_000541"
     CDS             2219..3460
                     /locus_tag="IW735_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1043564.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3569..4618
                     /gene="recA"
                     /locus_tag="IW735_000542"
     CDS             3569..4618
                     /gene="recA"
                     /locus_tag="IW735_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1043565.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5029..6585
                     /gene="rny"
                     /locus_tag="IW735_000543"
     CDS             5029..6585
                     /gene="rny"
                     /locus_tag="IW735_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1043566.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6664..7470)
                     /gene="proC"
                     /locus_tag="IW735_000544"
     CDS             complement(6664..7470)
                     /gene="proC"
                     /locus_tag="IW735_000544"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1043567.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7611..10184
                     /gene="mprF"
                     /locus_tag="IW735_000545"
     CDS             7611..10184
                     /gene="mprF"
                     /locus_tag="IW735_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1043568.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10338..11189
                     /locus_tag="IW735_000546"
     CDS             10338..11189
                     /locus_tag="IW735_000546"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1043569.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            11434..12372
                     /locus_tag="IW735_000547"
     CDS             11434..12372
                     /locus_tag="IW735_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1043570.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12395..13075
                     /locus_tag="IW735_000548"
     CDS             12395..13075
                     /locus_tag="IW735_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043571.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13048..13872
                     /locus_tag="IW735_000549"
     CDS             13048..13872
                     /locus_tag="IW735_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1043572.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14039..14857
                     /gene="purR"
                     /locus_tag="IW735_000550"
     CDS             14039..14857
                     /gene="purR"
                     /locus_tag="IW735_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1043573.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15360..16733
                     /gene="glmU"
                     /locus_tag="IW735_000551"
     CDS             15360..16733
                     /gene="glmU"
                     /locus_tag="IW735_000551"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1043574.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            16827..17798
                     /locus_tag="IW735_000552"
     CDS             16827..17798
                     /locus_tag="IW735_000552"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1043575.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      17804..17906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17972..18541
                     /gene="thiT"
                     /locus_tag="IW735_000553"
     CDS             17972..18541
                     /gene="thiT"
                     /locus_tag="IW735_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1043576.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18622..19395)
                     /locus_tag="IW735_000554"
     CDS             complement(18622..19395)
                     /locus_tag="IW735_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043577.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19661..21361
                     /locus_tag="IW735_000555"
     CDS             19661..21361
                     /locus_tag="IW735_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1043578.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21331..21549
                     /locus_tag="IW735_000556"
     CDS             21331..21549
                     /locus_tag="IW735_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043579.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            21569..22459
                     /locus_tag="IW735_000557"
     CDS             21569..22459
                     /locus_tag="IW735_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1043580.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22477..22761
                     /locus_tag="IW735_000558"
     CDS             22477..22761
                     /locus_tag="IW735_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043581.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23248..23862
                     /gene="gmk"
                     /locus_tag="IW735_000559"
     CDS             23248..23862
                     /gene="gmk"
                     /locus_tag="IW735_000559"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1043582.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            23867..24172
                     /locus_tag="IW735_000560"
     CDS             23867..24172
                     /locus_tag="IW735_000560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1043583.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24425..26833
                     /gene="priA"
                     /locus_tag="IW735_000561"
     CDS             24425..26833
                     /gene="priA"
                     /locus_tag="IW735_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1043584.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            26848..27339
                     /gene="def"
                     /locus_tag="IW735_000562"
     CDS             26848..27339
                     /gene="def"
                     /locus_tag="IW735_000562"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1043585.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27332..28270
                     /locus_tag="IW735_000563"
     CDS             27332..28270
                     /locus_tag="IW735_000563"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1043586.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28270..29628
                     /gene="rsmB"
                     /locus_tag="IW735_000564"
     CDS             28270..29628
                     /gene="rsmB"
                     /locus_tag="IW735_000564"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1043587.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29641..30381
                     /locus_tag="IW735_000565"
     CDS             29641..30381
                     /locus_tag="IW735_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1043588.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30378..32447
                     /gene="pknB"
                     /locus_tag="IW735_000566"
     CDS             30378..32447
                     /gene="pknB"
                     /locus_tag="IW735_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1043589.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32612..33511
                     /gene="rsgA"
                     /locus_tag="IW735_000567"
     CDS             32612..33511
                     /gene="rsgA"
                     /locus_tag="IW735_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1043590.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33524..34174
                     /locus_tag="IW735_000568"
     CDS             33524..34174
                     /locus_tag="IW735_000568"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1043591.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34175..34813
                     /locus_tag="IW735_000569"
     CDS             34175..34813
                     /locus_tag="IW735_000569"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1043592.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            34968..35822
                     /gene="mreC"
                     /locus_tag="IW735_000570"
     CDS             34968..35822
                     /gene="mreC"
                     /locus_tag="IW735_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1043593.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            35826..36335
                     /gene="mreD"
                     /locus_tag="IW735_000571"
     CDS             35826..36335
                     /gene="mreD"
                     /locus_tag="IW735_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1043594.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            36590..38128
                     /locus_tag="IW735_000572"
     CDS             36590..38128
                     /locus_tag="IW735_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043595.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT        12552 a         6828 c         8305 g        10491 t
ORIGIN      
        1 acggaaaaat atctccttat attttgccgg tttttgagct aataccgagg aacagctgat
       61 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttta actccgagtc acaaggaaca
      121 agttcaaaaa acagcttctt atatttattg gatattgtga cttgtgaccg aggagttggc
      181 aggagaacaa tgtttattcg gaaaaaagga gctgtgacat gacttttgtc acagcaccca
      241 aacataacgt ctttcgttgc aacagcagat gcttcggaga atcaaacaga ctggagaaga
      301 tgaacgagtg gcttttgaaa ccatcggaga aaaattacgg caagcacgcc tgaataaaaa
      361 aatatcttta gatgaattac agcagattac aaaaattcaa aaacgttatt tggaagcaat
      421 cgacaatgat gattttgacc aattgcctgg taaattttac gtgcgggcat ttattcggca
      481 atacgctgaa gctgtaggag aagatggaga ccatttagtc gatgtttttg atgggaaaag
      541 caaattgaca ggatcggtcc ctgaacgtcc gcagcctgaa acagtaagta cttctagaaa
      601 agcaatgcat caggaagaat cgcatccaag tcgcttttgg acaagtcttc ctgtcatttt
      661 attaggattg gttgctttgg cgattgtcgt tgtcgtgggc tatatgactt ggcaagatcg
      721 tcaagctgat ccaataatcg gagacacagc ttcttcgctt aaagtagaag gatcagtcga
      781 ggagtcctct tcctcagagc aaagcacgca gaccaaagag tcttctacag tagaatcaac
      841 aacaaaatca agtgaagaaa agaaaatggc aatagctaca ggagaagatt ctggcagtgc
      901 catcgcagta aatatcacag acgctaccaa gcctgtaacc cttgaattta ctgctgtcaa
      961 tcgtgtatgg ttaggtgtgc tagtagacaa tgcctatgtt tatcaaggga cactggcagc
     1021 aaatgaaaca cagagtaccg tgttaccgga aacagcaaca aatgcgacca tcacgattgg
     1081 tgcagcaagc aatgcaacga tcaaagcgaa tggagaatcc gtaccagtta atccagggga
     1141 aaacaatcag agtccaaaaa atgtgaacct tactcttcaa tacgcagaat gatcgtataa
     1201 attagtttac gcatctgttt acttgtaggt ataaaaaaga aggatcgtga caaatctgtg
     1261 tcgcggtcct tcttcgttaa ttcaaaaaga tcaatttcaa taaaaatact cctttgaata
     1321 aattcatttg gaaaagtaaa taatatcggt ggaaacttcg ctgatagtca cgtagaatag
     1381 ggaagaaagg agtcgatagt gtgaatttac caaataaatt aactgtactt agaatttgta
     1441 tgatcccgat tttcattttg gttgtaggtt tacctttaga ttggggacaa tttaacgtag
     1501 gagacaccac actggctgtc acacagctag taggtgcagt tatttttgct ttagctagta
     1561 tcacagactg gttagatgga aaaattgccc gtgcgcgagg ccttgtcact aattttggca
     1621 agtttgcgga cccgttggca gataaaatgc tagttatgac tgcttttatc atgttggttg
     1681 accaaggaaa ggcccctggt tgggtagtag cgatcattgt ctgtcgtgaa ttagccgtca
     1741 caggcttgcg tttactactt gtcgaacatg gagaagtcat ggctgcagct tggccgggaa
     1801 aagtcaagac ggctacacaa atggttgcta ttatcttgct gtttatcaat aatatcccat
     1861 tcagcctgat cggtattcca gtagatcaaa tcatgcttta tgcttgtctg atctttacta
     1921 tctattcagg tgtggattac ttcatcaaaa acagcggtgt gttcaaagga tcaatgtaag
     1981 tatcgtttag tggagaggtt gtgagcctgc cgtctaactc tgagtcacaa gggataattt
     2041 caaaaaacag cttttcatgt tttatgtata ttgtgactta tgaccgaggt tttggcagga
     2101 gaacactgtt tatttggaac aaggagctgt gacaagattt ttgtcacagc tccttgccct
     2161 ctttttaaat aaaaaaatat aaaaatacag cgtatactat ataagggagc gggaagtaat
     2221 gaaagcagaa ataatagctg tcggaacaga acttttacta ggacaagtcg tgaatacgaa
     2281 tgccactttc ttgtcagaac agctagctga tctaggtatc gaagtttatt atcaaacagt
     2341 tgtaggagat aatcagcagc gtttagaaga gttgattgct ctagctgaaa caagaagtga
     2401 actcatactc ttatgtggcg gattaggacc gacagaagat gacctgacaa aagaagcaac
     2461 tgcggctcac ctcggtaagt ctctcataca aaatacggaa ggatacaaaa aattattggc
     2521 atattttgaa acaacccatc gaaaaatgac caaaaacaat ctgcaacagt cccagattat
     2581 cgaaggcggg gttcctctac caaatcgtac agggcttgct ttagggactt tttatcaaac
     2641 agatacacat gcgtatatcc ttcttcccgg tccgcctaat gagttgaaac caatgtttgt
     2701 ggaacaagta cgtcctttac tagaggaacg ttttccttct gaagaaaaac taatttctaa
     2761 agttttgcgc ttttatggga ttggtgaatc gcgactggtc actgagctaa aagatctgat
     2821 tgagacacag atcaatccaa cgatcgcacc atatgccaaa ccaaatgaag tgacattacg
     2881 actgacagtg aaaacaaatg atgttcaggc agggaatcaa gctcttttag cattagaaga
     2941 aaaaatccaa gaacgagtag gggaatattt ttatggatac ggcgatgata attcgctagc
     3001 aaaagtcgta gtggaactac tgaaagaaaa caagcagact gtaacagcag cggaaagttt
     3061 gacagccggc gccttccaag ccgcattagg cgacattgct ggtgtatctg aagtatttcc
     3121 aggcggattc gttacctact cgctccaaac aaaagctggt tttctggaga ttgatccgga
     3181 gctattagca gaatatggaa cagtcagcaa agaatgtgtg gagcagatgg ccattcaagc
     3241 acgtgagata gcaagaacag attacagtgt agctttcacc ggagtggctg gaccggacac
     3301 gttagaaggt caatctgcag gaacagtatg gatcgctatt tcatctaaag acggcgtggt
     3361 ttcggaaaaa tatcactttg ccagagatcg tgctgctatc agatataatg cggttatgca
     3421 aggatttgat cttttgagaa aacagctttt aagaaaatga taaatgcgaa tatttgttcg
     3481 gttttttctt gctttttttc actaaaaaag atatgataag gataatgaaa tgttgctctg
     3541 atttgtaaat ataaggagga ttatcgtatt ggcagatgat cgtaaagcag ccttagatgc
     3601 tgcactgaaa aaaattgaaa agagctatgg taaaggctct attatgaaat tgggcgaaaa
     3661 aatcgaccaa caaatctcta caattccaag tggctctctt gcgttagatg tcgctttagg
     3721 tgtcggcggt tacccccgtg gacgtatcat cgaagtatat ggacctgaaa gttcaggtaa
     3781 aacaacagtt gcactacacg ctattgcaga agtacaaaaa aatggcggaa cggccgcttt
     3841 cattgatgct gagcatgcgt tagatccgca atatgcacaa aaattaggtg tgaatatcga
     3901 tgaactactt ctttcacagc ctgacacagg agaacaaggt ctagagatcg ctgatgcttt
     3961 agtatcaagt ggggctgtag atatcgtagt agtcgattca gttgctgctt tagttccacg
     4021 agcagaaatc gacggcgaaa tgggtgactc acatgtcggg ttacaagcac gtttgatgtc
     4081 tcaagcattg cgtaaactct ctggttcgat caacaaaaca aaaacaatcg ctattttcat
     4141 caaccaaatc cgtgaaaaag taggcgtgat gtttgggaat ccagaaatca ctcctggagg
     4201 acgcgcattg aaattctacg caacgatccg tttggaagta cgtcgtgcag aacaattgaa
     4261 acaaggtaca gatattgttg gtaaccgtac aaagatcaaa gtcgtaaaaa acaaagtagc
     4321 acctcctttc aaaatcgctg aagtagatgt catgtatggt ttagggatct cacaagaagg
     4381 agaacttcta gacatggcag tagaaaaaga cattgttgat aaaagcggtg cttggtattc
     4441 ttataaagaa gatcggattg gtcaaggacg ggaaaacgct aaaatctata tggctaatca
     4501 tcctgaaatg atggcagaag tttctgctct agttagagca gcttatggta ttggtgaaga
     4561 agtcgctgtc ccagaagatg aaaaaggaca agaagaattg cctttagtag aagaataaaa
     4621 attatgataa acaaaaacgg ctggtctgaa gagtaggtac tctccttgac ctgccgtttt
     4681 tttattgaat aaagaaatcg tgagttattt aattatacga gtgggtacat aaaataaaaa
     4741 tatctgttca tctacaaaga ataatttaat agaaaagttt ctcaatttaa aggtttacct
     4801 atatgaaact agtgtcattt attttaaaaa ccatcgttta atgaataagt agaaagccgt
     4861 ttctttgttt atttaaagtt gacactacgg ggtacaaaca ttagaataaa gttgtgcatg
     4921 ttttttgtac acatatatgc gaagatgcat attgacgagt ttagaataat ttatcataga
     4981 agctgtatag atgataaaaa tgaaaaagat agtatggagg tggattttat ggtattcaac
     5041 attctcctcg ctatcatcgg tttaattgtc ggtcttggtt tagggtttat ggtcgcaaag
     5101 tcacgtcacg agaaagaaat agctggtgca caatcatcag ctgctggaat catcgatagt
     5161 gcaaaaaaag aagcagaaac tctaaaaaaa gaagcattgt tagaagctaa ggaagaaaat
     5221 cagaaatacc gttctgaagt tgaaagtgag ctgagagaaa gtaagttaga attaaaatct
     5281 caagagaatc gtttgattca acgcgaacaa acgctgaatc gtaaagatga ctcgttagaa
     5341 aaacgcgaga actcacttga aagtaaagaa gaagcactta gttcaaaaca gcaattaatt
     5401 gaagagcgag aaaaagacgt tgaaaaaatg attgaagatc aacaaaaaga actagaaaaa
     5461 attgctggtt tatcaaaaga ggacgcacga gatgtcatca tgaaatcgac tgaagaagag
     5521 ctgaatcatg agttaactct aatggtaaaa gaatctgaac agcgtgccaa agaagaagca
     5581 gatcgcaaag cgaaaaactt attatcatta gcgattcaac gctgcgcagc agatcaagtt
     5641 tcagaaacga ctgtttcagt cgttaccctt ccgaatgatg aaatgaaagg tcgtatcatt
     5701 ggacgagaag gtcgtaatat tcgaacgctt gagacgctga ctggtatcga tttaatcatc
     5761 gatgatacac cggaagcagt tgttctttcc ggattcgatc caatccgaag agaaatcgca
     5821 cggatgacct tggaaaaact gatacaagat ggacgaatcc atccagcgcg tatcgaagaa
     5881 atggtcgaga aatctcgtaa agaaatggat gaacgtattc gcgaatacgg ggaagaggca
     5941 gcgtttgaag tcggtgctca tacacttcat ccagatctaa tcaagatcat cggacgtctg
     6001 cgattcagaa ctagttatgg acaaaacgtc ttgaagcatt cggtcgaggt tgcaaaatta
     6061 gctggtatct tagcagcaga gcttggcgaa gatatccaat tagcaaaacg tgccgggttg
     6121 ctccatgata ttgggaaagc acttgaccat gagattgaag gatctcacgt tgagatcggt
     6181 gcggaattag ctgccaagta caaagaaaac ccagtagtaa tcaacgcgat cgcttctcat
     6241 catggagatg tggaagctac ttctgttatc tctgtccttg tagctgcagc agatgcgctc
     6301 tcagcagcaa gacctggtgc gcgaagtgaa tcattagaaa actacatccg tcgtttaaga
     6361 agtctggaaa atatttcgaa cagcttcgcc ggtgtagaat caagttacgc agttcaagct
     6421 ggacgcgaag ttcgggtaat ggtcaagcca gaagaaatct cggatttaga cgctgttcgt
     6481 ttagtacgtg atattcgtaa acgaatcgaa gaagaactgg attatcctgg acacatcaaa
     6541 gtaatcgtta ttcgggaaac tcgtgcaact gattatgcta aataataaaa aatacaaaag
     6601 aagagccgcg gcaaaagcca cagctcttct ttttttgtaa ggtacgaaaa aacattgcta
     6661 tcattatttt ttattcaaca tctcaagatc tttctcgatc gtcgtattga ttcctttgat
     6721 gattgctggg atgaatcctg cttcttctaa ggcaactaca cctgcaacag ttgtaccgcc
     6781 aggagaagaa acttggtcta caagttccca tggattttta tcacttgaaa ggaatttttg
     6841 acaagaaccc ataattgctt ttgcagcaat ctcagtggct gtctgtttgg acaaaccatt
     6901 cagtacacca gtccgtccca tcgcatcgat cagcatgtaa acgaatgcgg gtgagctgcc
     6961 agcaaggcca atgaatgtac tgaaatctga ttctgctaaa ggatagacgc tgccgattgc
     7021 ttcaaaaatc atcgtagctt gctgaagctg ttcctccgtg gcagcttcat ttttgcaaat
     7081 cgcagaaacg cctgctccta cgctgacatt gacattcggc atcacacgta cgatggaggc
     7141 ctgaggatct tcaagcgctg tttctaaatc tgttaatgat tttcctgaag caatagaaat
     7201 gaataagact ttctggctat attctcttag ttcttccaat actttttcaa tgatttttgg
     7261 tttcacggct aaaagaacga tatctgattg tgatgcaact tcctgattac ttttgcatgc
     7321 atggacgcca gtttcagcag caaatttttg tgcattttca aaatgtccac tggaaacgaa
     7381 cacctcttct ttcgagactg ttcctttttt taataaacca tcaataatag cttgtgccat
     7441 attccctgtg ccgataaatc ctactttcat gttatcctct cctttagcat taggatatct
     7501 ttctttttct atcatcgcaa gcaaagaaca ggtctaaagt ggaaaaatta aaataaataa
     7561 gataacattg gggtatgaca caaaagtata caagttagga atgggaaaat gtgaaaaata
     7621 aacgatactt gatgctgcaa tggataaagg aacatagtct gattttaaaa ctgatttttt
     7681 tcggctccgt gttagtcttt gtggcaaatc aagtcgcaaa tattgctaat ggcatgagtt
     7741 ggcaagatat ttggcaaagg atgggacaac aaagccgcat gacgatcctg ctcatgattc
     7801 tggctggact gctgggtgtc actccgatgt tgttgtatga ttgggtgacg atcagagtct
     7861 tagaaaaaca aggaaaacca aaaatggaaa gaaaagagtt tcttcttgct gcgtgggtaa
     7921 caaacacgat caacaatctc gccggttttg gtggtgtggt aggagcaagt ctgcgggcta
     7981 gtttttatgg aaagcagaca aatagaaaaa tggttttagc cactgtatca aaagtcgcac
     8041 tatttatgct gactggtttg tctgtatggt cgtttcttac atttattgat gtctttttta
     8101 ttcagtcaga aagtattttt cgaagctatt ggatctggct tttcggcggt agttttttag
     8161 caccagtcat tttctttttt gcttatctaa agagaaagac attgttcttc gagttttatc
     8221 caaaaggctt gcttggtctg ctactggcat cctttggaca atggacggga gcccttgcgg
     8281 tatttttgat catcggcaag ttgatgagaa tagatatttc gatgctttcg atctatccta
     8341 tgttcatgat tgctacattg ataggaatgc tgacgatggt cccaggtgga atggggactt
     8401 ttgacgtttt gatgatctta ggaatgagtc aattgtctgt tcggcaagat gtagcagtag
     8461 tttggctatt atattatcgg ttgttttatt atgttctacc ttttatgacg ggacttctat
     8521 tgttcatcac acatacagga gtcaaaatca atcgattttt agataacatt ccgcgactgt
     8581 ttttccaaaa ggttgcgcat atcgtgctcg taagtgctgt ctattttgcg gggatcatga
     8641 tggtacttct atcaacaatc actaatctgt ctgcaatcag tcggctattt caagtattat
     8701 tgccgttctc attcaatttt ttagatcaga cagtgaatct attggttggc tttttattac
     8761 tcggccttgc tagagcactt tcagtaaaag tcaaaaaggc atttctgcct acgattgctt
     8821 tactatcttt tggtatttta aatacaatca cacgtacgtt atcttggcag ttgatattgg
     8881 tatatgtctt gattctgtta gctgtgtggt tatccagaaa agaattttat cgaacaaaat
     8941 tcgtttattc ttggggagct ataatctttg atgctagctt gttcggcttt ttgtttatcg
     9001 tttacgcaat tgcaggctat cacagcggac gctttttagt cggtcgaata acaggaaatc
     9061 attttatttt gtttccttca gatgatgtgt ggttctcagg gcttattggt ttgggcatct
     9121 cattgctagc tttagtggcg ctttatcaat atttagctga agatactacg aagcttggca
     9181 gtgagtacga aaaagaccgt tttgaccgtt tattggaaac atacggaggc acagctacaa
     9241 gccatcagct tcgcttgtca ggttacagtt atttttatta ccaagaagac gaccaagatc
     9301 aagtcgtttt tggttatcag atcaaaggaa acaaatgctt tgttttagga gatccagttg
     9361 gcaatcctaa aatgatgcgg gcagcaacga atcagtttat gaaacaagca gattgtttag
     9421 gctatcagct tgcgttttat aagatatcag aaagctacgt agtgatgctt catgaattgg
     9481 gttttcactt cacgaaagta ggtgaagcag ggattgttga tctagcaaca gaagacttgt
     9541 ctcggctgag tcagcaactt gaatatcaac gtttgatgaa agaaggatac cattttactt
     9601 ggtacgatca actgccggaa gaattacatc aagctgtaaa tgaagtttca caaaaatggc
     9661 ttcaaggtgc acgagaaaaa tacttctctg tgggacgatt tgataaatca tatgtattat
     9721 ctagcggtgt cggtattgtc cgtaaagatc aaaaagtcgt tggcttcatc accgctcagc
     9781 cgatgacgaa gaagcaggcg ggatatgatt tgttgcgggt cttacctaaa gaatctgagg
     9841 aattagctga ctatctgtta gccaatctgt ttattgtata cgagaaaatg ggctactcgg
     9901 aagccaatct aggcttagct ccattagcta atgttggaga aacagatttt tctttctttc
     9961 aagaaaaagt catgcatatc gtatataatt atggtaattt tttctattcc ttccagtctg
    10021 cttatgaaaa gaaattaaaa tatgtcgatc attgggaagg acgttatttt gcttatagga
    10081 aaggaagtaa tttcggattt gctaccatgc agctactcag tctgatagga aaagaaaaag
    10141 gaaatgttcc gagtttggca gaagaagtgc tcacagaatt ataagaatca ctagaacttg
    10201 tttggattat gaagagaaaa ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact
    10261 aggcagacat aagctatact tttatttttc cctatgatag aatacttgcg acttagactt
    10321 ttcgaatggg tgagaggatg gaattgattg aaaaagcgcc agccaagata aatttaggac
    10381 tggatgtatt atatagacgg caagatgggt atcatgagtt ggaaatggtg atgtcgagcg
    10441 tagacttggc agatcatcta ttttttgaag aaataacaga agacaaaatc attgtagaaa
    10501 caaataaagc atttttaccg gtagatgaac ggaacaatgt ttatcaagct gctttattgc
    10561 taaaaaataa atacaatatc aaaaccggtg tcaagatctt tgttaagaag aatattccag
    10621 tagcagctgg attaggcgga ggaagtacag actgtgcagc tgctctaaga gggatcaacc
    10681 aattatggaa tctgggctgt tcgttagaag aactggcaga aatcggtttg gaagtcggta
    10741 cagatgttcc ttactgtgtc tacggaagta cggcatttgt cggtggcaaa ggggaagaaa
    10801 tccaagtatt accttctatg ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg
    10861 tctctacacg tacagtgttt agagaagtag atacgcaagc catccaacat caagacatcc
    10921 acggacttgc tgaagccatc cgagagcaag attatcagca gatgatcaaa aaaatgggaa
    10981 acagtttaga agatataacg ggaaagcggc atccagtcat ccatcaaatc aaagagcgta
    11041 tgatcaagta cggagcagat gctgcattga tgagtggaag cggaccaacc gtttttgcct
    11101 tgtgtcaaca ttattcaaga gcgcagagag tatataatgg gttaaaaggt ttttgtgagg
    11161 aagtttattt agtacgaacc ctgcaataaa atataagaag taaaataggt gatgaaaaac
    11221 tgaggaatcc taaaatggta ttcttcagtt ttttattgta gtgaaatggc tagatatgag
    11281 cagcaactca tccattttac gatttaatca agaatataca tgcttaaatc agaaattgat
    11341 ggatttctat tgactgtgtt tttttatttt gatacattag ttcacgttaa taaatcgtaa
    11401 aattccgatt tgttcattga aaggagcata taaatgaaaa aatatctttt gacagctacg
    11461 gtaatgatag gtgctttact gtttgcagca tgtggaaata cgaacaagga agcagacaaa
    11521 aaagaggatt tgacgatcgt tacgactttt tatccgatgt atgattttac aaaagagatc
    11581 gtaggggatg aaggaaacgt gaaacttttg atccctgctg gaacggagcc gcatgatttc
    11641 gaaccaagtg caaaagaaag agcagaaatt tccgatgcag atgttttcgt gtataatagt
    11701 tcagacatgg aattttttgt ggattcgtta aaggattcag tggatagcaa acagaccttg
    11761 atgatcgaag cagctaaagg aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa
    11821 gaatcagaag aagggcatgg tcattcacat gaatacgatc cacatgtttg gctcgatccg
    11881 gtattagcta taaaagaagt cagaacgatt gctgaagaat taggcgaaaa atatccagat
    11941 aaaaaggaaa cttttacgaa gaatgccgat gcttatatca aaaagcttga agcactggat
    12001 caaaaatact ccgatgagct aaaagatgca acaaaccgca catttgtcac tcagcatgct
    12061 gcttttgctt atttagcgaa tcaatacgac ttaaaacaag tagcgatttc gggcgtttca
    12121 ccagatcagg aacccacacc aagtcgctta gctgagctaa aagaatttgt gaagaaaaac
    12181 aacatcaaag tgatttattt tgaagaaaat gcctcttcga aggtagctga gacattgtcg
    12241 aatgaaacag gagtgaagct agaagtgttg aatcctttag aaagcttgac taatgaacaa
    12301 atcaaagcag gcgaaaatta tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa
    12361 agtatcaatt aagttttaga atggaggttg taaggtgcgt tatatcgaaa tatctgattt
    12421 gaccttttac tatgatgatg aacctgtact agaagatgtc tcttatcatg tagatgcggg
    12481 tgaatttgtc attttaactg gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc
    12541 gttaggatta ctgaaacctt caaaaggaat gataaagatt gctgagaaaa atcaagaagg
    12601 aaaaaaatta agtatcggtt atattcctca acaagttgca tctttcaatg caggttttcc
    12661 tagtacggtg atcgaattag tccgttcagg aagatatcca agaggccgct ggttcaagcg
    12721 tctctctgaa aaagatcatc aacatgtaca gcgtgcttta gaagctgtag ggatgtggga
    12781 tatgcgaaat cggcgaatag gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc
    12841 gcgtattttt gctactgacc ctgacttatt tgttcttgat gaaccaacaa caggtatgga
    12901 tgaagaatcc agaaatgaat tttatcgtct tctccgacat agtgcgcatg tccatgggaa
    12961 atcagttcta atgatcaccc atgatcatga agatatcaaa cagtatgcgg acagacagat
    13021 ccgactggtg agaaaggagg attctcaatg gcgttgcttt catatgaatt catgagaaga
    13081 gcgtttcttg ccgctatctt cattgccgga atcgccccga tgctaggtgt gtttttagtg
    13141 atcagaagac aatcgctgat ggctgatacg ctttctcatg tctctttagc aggggtagcg
    13201 ttaggattct ttttgaacct taatcctaca cttacgacca tgctagtcgt tgtattagca
    13261 gctgtgatat tggagtatct gcgaacgatc taccgaacct attcagaaat ctccattgcc
    13321 attttaatgg ctggtggttt ggcgctagcg ttagtattga tgaatttgag cggagggaat
    13381 tcagcaacaa gtatccaatc ctatctgttt ggttcgatcg tgaccattac atgggagcaa
    13441 gtcatctttt taggaatctt attcgttgcc atcggtatat tgttcttttt attcaaacgg
    13501 ccgatgtatg ttctgacttt tgatgaagat accgcccacg tggatgggct acccgttcat
    13561 ttgatgtcga tgatgttcaa tgtgattact ggtgtggcta ttgctgtgat gatcccgatt
    13621 gccggtgcac ttttgatatc cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta
    13681 ggcaaaagct tcaatgtcgt gattgctatc agtgtttgtg taggatttct agggatgctg
    13741 tcaggactaa ctagctcgtt ttatctagat acacctccag gagcaacaat cacattagtc
    13801 tttattgctt tgttcctgat tgtaaatatc atcaaacgaa tattggttgc cttcagaaga
    13861 aaaaataact aaattataaa gaaaagaagc cttataaaaa cgaaaaattt tttataggct
    13921 tctttttatt ttaataaata ccgaactatt ttcttatttt ttcataaaat gctcgaattt
    13981 ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt
    14041 gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca
    14101 tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag
    14161 tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac
    14221 aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa
    14281 aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg
    14341 atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat
    14401 cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg
    14461 cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg
    14521 ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga
    14581 aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt
    14641 agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga
    14701 attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg
    14761 cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa
    14821 tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag
    14881 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc
    14941 ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc
    15001 tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga
    15061 gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt
    15121 ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg
    15181 tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat
    15241 ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg
    15301 aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt
    15361 tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat
    15421 tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg
    15481 tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta
    15541 aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac
    15601 atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca
    15661 gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag
    15721 gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac
    15781 gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac
    15841 cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt
    15901 tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag
    15961 atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt
    16021 ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga
    16081 gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga
    16141 catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa
    16201 tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag
    16261 acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca
    16321 agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa
    16381 acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag
    16441 tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg
    16501 gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca
    16561 gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga
    16621 ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag
    16681 caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt
    16741 gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag
    16801 ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa
    16861 tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg
    16921 agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag
    16981 aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata
    17041 acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca
    17101 acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac
    17161 caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga
    17221 ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga
    17281 tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg
    17341 tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga
    17401 aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga
    17461 atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag
    17521 caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg
    17581 cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta
    17641 tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa
    17701 tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca
    17761 aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag
    17821 tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga
    17881 gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt
    17941 gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg
    18001 aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag
    18061 tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa
    18121 ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta
    18181 ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc
    18241 agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat
    18301 tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt
    18361 tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatt cttggctctt
    18421 ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt
    18481 attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta
    18541 aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt
    18601 attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac
    18661 caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg
    18721 ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata
    18781 tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt
    18841 acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa
    18901 agattttttt acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct
    18961 ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta
    19021 tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc
    19081 aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt
    19141 aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg
    19201 ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc
    19261 agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa
    19321 gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata
    19381 aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac
    19441 atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc
    19501 attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt atttaaagga
    19561 tgttttatga attgttttct tatggaatct acaaagtcga atacgagact catgaccttt
    19621 ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta
    19681 cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt
    19741 ttttcaaagt catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc
    19801 ttttaaagca gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga
    19861 tgttattttt aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg
    19921 gtatatggaa gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc
    19981 tcctctatca gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca
    20041 attaaataaa aagctggtct atcatacagc acttgtttta ggtggaggag gagcacacgg
    20101 tgcttatcag attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat
    20161 cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa
    20221 agctgtgaat ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc
    20281 tgcagttgaa gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc
    20341 actaattgaa ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga
    20401 tttgtcctta ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc
    20461 agaactagcg gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg
    20521 gatactcgcc tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa
    20581 atatgcagac ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc
    20641 aacagaggca tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc
    20701 agacattttt atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt
    20761 tgatagccaa aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt
    20821 aggctattac tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg
    20881 gcgaggattt ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag
    20941 cataaaattt tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg
    21001 cggattggcg atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta
    21061 tcaagtagac atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga
    21121 tttattggca caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca
    21181 gcaaaaaata caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa
    21241 acgcaaggat cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct
    21301 atatttatat tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta
    21361 gaagaaatcg ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc
    21421 agctggaatg gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa
    21481 atggggaaag gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa
    21541 aatatgtaat gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat
    21601 ccgcgaaaca aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct
    21661 agatgttcaa atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct
    21721 tattaaacag cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg
    21781 caaaaacgaa aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt
    21841 gacagacgga attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc
    21901 ggaacgcttc gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat
    21961 ggaagaattg gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc
    22021 gagtcgagaa aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt
    22081 caaagaagta ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat
    22141 aaggtatcaa aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt
    22201 gacggagcta gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc
    22261 gatccatgta caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt
    22321 agatttcctg atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc
    22381 aatcgaaatt aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca
    22441 agtacaaaat attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc
    22501 cacgaattaa gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt
    22561 gacgtatgga aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac
    22621 gacggtgctt acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta
    22681 gaagatccga cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact
    22741 ttgtccaata tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa
    22801 ttggatttct tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt
    22861 cgtgtcacaa tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt
    22921 aagccaaatt tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga
    22981 aactaatcgg cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata
    23041 ataaccccaa ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt
    23101 gatgcaggac agctttttta cgacctcttg gtacagggta gcttttttac caatttctag
    23161 ctataattaa agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac
    23221 aaaaagacta gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct
    23281 ggtccatccg gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat
    23341 gattttcagt attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc
    23401 gttgattatt tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg
    23461 ctggaatacg cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag
    23521 acattggatg aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc
    23581 aaagaaaaag tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta
    23641 aaatcacgga tcgtaggccg aggaacagat gcagatgaag taatcgaaga acgaatgaaa
    23701 gttgcaaaag aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa
    23761 gtgccgctag cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat
    23821 cgcgtaatcg gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc
    23881 ttcaattgac tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag
    23941 taaacgcgcg catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt
    24001 aaaaagcgtc ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca
    24061 tcctgaatta aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga
    24121 gcgtgaacaa cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa
    24181 aatagagcga acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc
    24241 acttattgtc tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt
    24301 atctgctgtt aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta
    24361 gtaaaatagg caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg
    24421 aaccatgaca ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc
    24481 attcacttat ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga
    24541 agtccctttt ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc
    24601 agaagaatca gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt
    24661 ggtcaatgaa gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa
    24721 aatcacttgt ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat
    24781 ctatcctttg gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc
    24841 ctggaaggaa gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca
    24901 attagtagat atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt
    24961 ccgttctttg ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc
    25021 caagaaacaa aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc
    25081 ttatttcaaa aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg
    25141 gctggagttt atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca
    25201 aacgacggca ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc
    25261 aggtcagcag caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac
    25321 agaagtatat cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt
    25381 agtgcctgaa atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga
    25441 atcagttgcg gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa
    25501 gattgagcgg gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct
    25561 gaaaaatatc gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga
    25621 gacaccgcgt tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc
    25681 aatagttcta ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt
    25741 ttatcagtta ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga
    25801 gatcgtagat atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt
    25861 gcaggaaaaa atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg
    25921 tcgaggatat tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa
    25981 ttgcgatatc tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg
    26041 acatgaagaa cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta
    26101 tggaacaggt actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat
    26161 tttgcgtatg gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa
    26221 attcggtgca aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga
    26281 ctttccagaa gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga
    26341 ttttcgttct agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg
    26401 agcggaaaaa gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga
    26461 attggctaaa aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg
    26521 tggaggctat cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca
    26581 agtagcggca aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca
    26641 aagcttgcta ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta
    26701 tcaactaatc atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt
    26761 gaatggaagc cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt
    26821 aaactttatt taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat
    26881 aaattaaaaa gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta
    26941 gacaatttat atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta
    27001 ggacaaaaca agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt
    27061 aacccagaaa tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt
    27121 ccgcatgtct atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga
    27181 gacggagaag agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa
    27241 atcgatcact tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa
    27301 gaacttgaag aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc
    27361 tgctttttct gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt
    27421 ggtaacacag cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa
    27481 agaagcggct ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc
    27541 agaaatggaa gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca
    27601 attcttgcct gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc
    27661 tttacttcct aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa
    27721 agaaactggt gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc
    27781 gcaagaaagc ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag
    27841 ccttttagga agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc
    27901 agtacctcaa gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc
    27961 aatcgattgg cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg
    28021 gccaattgca ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt
    28081 ggcagaaact acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg
    28141 gattgcttgc ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg
    28201 aaaacaggcc gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca
    28261 ggtgaaataa tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta
    28321 ctgacattag agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg
    28381 atcaaacaaa gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt
    28441 acgattagcc atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa
    28501 aaagtggatg actgggtcaa gacgcttctt tatctttctg tataccaatt agaattttta
    28561 gataaagtcc ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga
    28621 aatcctggga cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca
    28681 ccagatctgc aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct
    28741 ctatggatga ctaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt
    28801 ttatcgctat atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga
    28861 gatcaggcca tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt
    28921 ccatacggta tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc
    28981 gtcatgacga tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct
    29041 gaacatcatg tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct
    29101 tttttagatc cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc
    29161 aaactcattg aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag
    29221 atggatgcta gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta
    29281 gacgcgcctt gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa
    29341 cagccggatg aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca
    29401 ccaactttaa agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa
    29461 aaccaagacg ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt
    29521 ttgaccaatc ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat
    29581 cagttttaca cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa
    29641 atgcagattg aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc
    29701 agtgtattta ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt
    29761 catcgtgccg gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa
    29821 aaacaagaag ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa
    29881 gaaaatatgc tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca
    29941 acaatcgtag cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc
    30001 agagcttatt tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc
    30061 aatgaactag tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa
    30121 aatattttaa cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat
    30181 ctttggcaac taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct
    30241 gaagaagaaa taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag
    30301 ctgattgatc gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa
    30361 tataaggagg acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca
    30421 ctggaaatat cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg
    30481 atcgtgatgt agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc
    30541 gacgatttca gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg
    30601 tttacgatgt tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa
    30661 tggacttgaa acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata
    30721 ttatgcagca gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag
    30781 atttgaaacc ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg
    30841 ggattgctat cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg
    30901 ttcattatct gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt
    30961 atgctttagg gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat
    31021 ctgctgtaac aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg
    31081 atccaaatgt gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg
    31141 cagaccgtta taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag
    31201 ctcgtgcaaa cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca
    31261 caccccttac ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag
    31321 acaagccaga aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga
    31381 aaggacagaa aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa
    31441 cagctgtatt ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc
    31501 tttctgaagc ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa
    31561 cagaagaaat tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag
    31621 cgggaacgac agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa
    31681 aaatcaaatt agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag
    31741 aattagggtt ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa
    31801 gcggaatgat catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca
    31861 cgattacttt cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag
    31921 aaaactacga aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta
    31981 gtgctactta tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag
    32041 cctcgggtga agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag
    32101 agcctaaatt gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta
    32161 gtgtcggtgc agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag
    32221 ataaagtgat tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga
    32281 acgtaattta ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag
    32341 acagttctag cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt
    32401 ccagctccac aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa
    32461 tatatcagaa gggaatgcga cattgttttg ttgcattctc ttttttgtgt ttctatctct
    32521 tttgatcaat tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc
    32581 tcttcgtatg gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa
    32641 agcaatcagc ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag
    32701 aggaaatttc cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgagag
    32761 tgaaaatcaa acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg
    32821 cccaccagtc acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt
    32881 ttcttataat ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat
    32941 tatttttttg acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca
    33001 aacctatact aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga
    33061 actgatccgt tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa
    33121 atcgacgcta ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga
    33181 tgcacttgga agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg
    33241 gttagtagca gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga
    33301 actgccgaga caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg
    33361 catgcatgct aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc
    33421 acagacacgt tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat
    33481 gtatgcaaaa aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc
    33541 cattttgagt gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg
    33601 cgcagattac atccacgtag atgtaatgga tggccaattt gtcccaaata tcacattagg
    33661 gccaaatatt gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat
    33721 gatcgttgat ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac
    33781 tgtccacgtc gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg
    33841 agttaaagct ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt
    33901 gcctttggct gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt
    33961 tattgaagaa acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg
    34021 gacttatgca atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga
    34081 tgcaggggcg gatgtcttcg ttgcagggtc atatatctat ggagccgaaa atccagtcgg
    34141 acaaatcgaa cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg
    34201 taatccgcaa aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga
    34261 ccgtggtgcg ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt
    34321 tgattcattg tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg
    34381 ttcccctgca gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg
    34441 ttacccgcaa gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt
    34501 agccaatctc tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat
    34561 cagagattgt caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat
    34621 accagatatg aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa
    34681 aggcagcaag tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa
    34741 cgaatttctt tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca
    34801 aagcaaagat tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga
    34861 atctattatt tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt
    34921 tagtgataaa attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca
    34981 atcctaataa aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa
    35041 gtgtgaccgc tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta
    35101 atgacagtgt tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg
    35161 gagtagattc tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa
    35221 aaatcgatag ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag
    35281 cgttgaaaaa agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa
    35341 atgtcattac tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta
    35401 aagacggtat cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga
    35461 tcgaggtcaa tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca
    35521 atcattttcc agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact
    35581 atgatgaaaa gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag
    35641 aaggggatgt tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg
    35701 gtacggttat taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac
    35761 cttatgcaga aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt
    35821 agtccatgtt gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga
    35881 tgttgataga tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt
    35941 caaatgccca ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt
    36001 atttactgat taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta
    36061 tcggtatcta tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg
    36121 ttatccatgt caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg
    36181 aattgtttac gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt
    36241 ttattacaag gtttttagga cctacactgc tgttgaacat gattatattt gtattattca
    36301 tttttccctt taagaaatta ttcagtgatg aataatttct ttttttattt ttaggtcgaa
    36361 agcgaaaaag aatcgttgtt atcttaagaa atgctagact taacgcggct ttttcatgat
    36421 cattttctga aacgaacagt tttgtcattt ttaaaagttg ttttgaaaca tttttgtaac
    36481 ataactatta tatcgttgac acactgctat ggtacaattt ttatatcgtt aagaaagact
    36541 taaatatatt tagatattta ttaaattaag ccagtcggag gaatgaagag tgaaaaagag
    36601 tttaatatca gcagtaatgg tatgttcaat gaccttgact gccgtagcat caccgatcgc
    36661 tgccgcggcg gacgattttg attctcagat acaacagcaa gatcaaaaga ttgcagactt
    36721 gaaaaatcaa caagcagatg cacaatcaca aatcgatgcc ttagaaagcc aagtttctga
    36781 aatcaacaca caagcacaag acctattagc aaaacaagat acattgcgtc aagaatctgc
    36841 acaattagta aaagacattg cagatttaca agaacgtatt gaaaaacgtg aagatacaat
    36901 ccaaaaacaa gcacgcgaag cacaagtaag caacacaagc tcaaactaca tcgatgctgt
    36961 attgaatgct gattcattag cagatgctat tggacgcgtt caagcaatga caacgatggt
    37021 aaaagcaaac aacgacttga tggaacaaca aaaacaagat aaaaaagctg tagaagacaa
    37081 aaaagctgaa aacgatgcta aactaaaaga attagctgaa aaccaagctg ctttggaatc
    37141 tcaaaaaggc gatcttttat ctaaacaagc tgatttgaac gtactgaaaa cttcattagc
    37201 tgctgaacaa gcaactgctg aagacaagaa agctgacttg aaccgtcaaa aagcagaagc
    37261 tgaagcagag caagctcgta ttcgtgaaca acaacgtcta gcagaacaag ctcgtcaaca
    37321 agctgcacaa gaaaaagctg aaaaagaagc acgcgaacaa gcagaggctg aagcacaagc
    37381 tactcaagct tcatctactg cacaaagctc agcaacagaa gaatcatcag ctacacaatc
    37441 ttcaatgact gaagaatcat catcagctac acaatcttca gcaacagaag aatcaacaac
    37501 acctgaatct tcaacagaag aatcaacagc gcctgaatct tcagcaacag aagaatcaac
    37561 aacagtgcct gaatcttcag caacagaaga atcaaaaaca gtgcctgaat cttcaacaac
    37621 agaagaatca acaacacctg cgcctacaac accatcaaca gatcaaagtg ttgatacagg
    37681 aaacggcaca ggaagtagta ctccggctcc aacgccaaca ccaacacctg aacaaccaaa
    37741 acctgtaaca cctgctccag ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga
    37801 agcttacaaa tatattggta ctccatatgt ttggggcggt aaagatccaa gtggctttga
    37861 ctgctcaggg ttcacacgct atgtttacat gcaagtaact ggtcgtgaca tcggtggttg
    37921 gacagttcct caagaatcag ctggaacaaa aatttcagtt tctcaagcaa aagctggaga
    37981 cttgttattc tgggggtcac aaggcggaac ttaccacgta gcgattgctt taggcggagg
    38041 acaatatatc catgctcctc aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt
    38101 tgcccctgac tttgctgtca gcatgtaaga atgattaatc aaaaagagtt agcccattct
    38161 ggactaactc tttttt
//
LOCUS       DACEMS010000012        35911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEMS010000012 DACEMS010000000
VERSION     DACEMS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.012"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..2478)
                     /locus_tag="IW735_000573"
     CDS             complement(133..2478)
                     /locus_tag="IW735_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1043596.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2757..3659
                     /locus_tag="IW735_000574"
     CDS             2757..3659
                     /locus_tag="IW735_000574"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1043597.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3870..5645
                     /locus_tag="IW735_000575"
     CDS             3870..5645
                     /locus_tag="IW735_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1043598.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5711..7297)
                     /locus_tag="IW735_000576"
     CDS             complement(5711..7297)
                     /locus_tag="IW735_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1043599.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7548..7772
                     /locus_tag="IW735_000577"
     CDS             7548..7772
                     /locus_tag="IW735_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1043600.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7914..9665
                     /locus_tag="IW735_000578"
     CDS             7914..9665
                     /locus_tag="IW735_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1043601.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9665..11449
                     /locus_tag="IW735_000579"
     CDS             9665..11449
                     /locus_tag="IW735_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043602.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11784..12636
                     /locus_tag="IW735_000580"
                     /pseudo
     CDS             11784..12636
                     /locus_tag="IW735_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12733..14619
                     /locus_tag="IW735_000581"
     CDS             12733..14619
                     /locus_tag="IW735_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1043603.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14660..15562)
                     /locus_tag="IW735_000582"
     CDS             complement(14660..15562)
                     /locus_tag="IW735_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1043604.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15792..17195
                     /locus_tag="IW735_000583"
     CDS             15792..17195
                     /locus_tag="IW735_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1043605.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17195..18628
                     /gene="ascB"
                     /locus_tag="IW735_000584"
     CDS             17195..18628
                     /gene="ascB"
                     /locus_tag="IW735_000584"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1043606.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18773..19699
                     /gene="trxB"
                     /locus_tag="IW735_000585"
     CDS             18773..19699
                     /gene="trxB"
                     /locus_tag="IW735_000585"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1043607.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19912..21639
                     /locus_tag="IW735_000586"
     CDS             19912..21639
                     /locus_tag="IW735_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1043608.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21712..22005
                     /locus_tag="IW735_000587"
     CDS             21712..22005
                     /locus_tag="IW735_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1043609.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(22099..22959)
                     /locus_tag="IW735_000588"
     CDS             complement(22099..22959)
                     /locus_tag="IW735_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1043610.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23051..23479
                     /locus_tag="IW735_000589"
     CDS             23051..23479
                     /locus_tag="IW735_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1043611.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23641..23817
                     /locus_tag="IW735_000590"
     CDS             23641..23817
                     /locus_tag="IW735_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1043612.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23844..24290
                     /locus_tag="IW735_000591"
     CDS             23844..24290
                     /locus_tag="IW735_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1043613.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(24446..25825)
                     /gene="brnQ"
                     /locus_tag="IW735_000592"
     CDS             complement(24446..25825)
                     /gene="brnQ"
                     /locus_tag="IW735_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1043614.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            26530..27501
                     /locus_tag="IW735_000593"
     CDS             26530..27501
                     /locus_tag="IW735_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1043615.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            27525..29717
                     /locus_tag="IW735_000594"
     CDS             27525..29717
                     /locus_tag="IW735_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1043616.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            29746..30222
                     /gene="ybeY"
                     /locus_tag="IW735_000595"
     CDS             29746..30222
                     /gene="ybeY"
                     /locus_tag="IW735_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1043617.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            30200..30601
                     /locus_tag="IW735_000596"
     CDS             30200..30601
                     /locus_tag="IW735_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1043618.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            30616..31515
                     /locus_tag="IW735_000597"
     CDS             30616..31515
                     /locus_tag="IW735_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1043619.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            31658..32476
                     /gene="recO"
                     /locus_tag="IW735_000598"
     CDS             31658..32476
                     /gene="recO"
                     /locus_tag="IW735_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1043620.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            32862..33779
                     /gene="glyQ"
                     /locus_tag="IW735_000599"
     CDS             32862..33779
                     /gene="glyQ"
                     /locus_tag="IW735_000599"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1043621.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            33781..35856
                     /locus_tag="IW735_000600"
     CDS             33781..35856
                     /locus_tag="IW735_000600"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1043622.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        11549 a         6483 c         7665 g        10214 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag attggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acagtcaatg attgaaacac
     2401 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
     2461 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
     2521 aaataatagg acaaaaaaac agacaaggct gtttttgaca ggaatttctt tttcgaatga
     2581 gataaaacgt gaggaaatga tcttttttat gaaagagttc ttaagctttt ctttagggaa
     2641 agtttcagtt actatggaga acagggcaaa gaaaaacaaa ccattttctt tgtacatcat
     2701 cttatcaata tacaggaatt gttcgaaaca attgcctttc aaggaggaat ccatccatgt
     2761 tattaggctc acatgtcagc atgagcggaa agaaaatgct gttaggcgcg gcagaagaag
     2821 cagccagtta cggatcttcg acctttatga tctatacagg cgctcctcaa aatactagaa
     2881 gaaaagctat cgaagagatg aatattgatg ccggaaaagc atttatggca gaaaacggac
     2941 taacaaatat cgtcgttcat gcgccttata tcattaattt gggaaatacg atcaaaccag
     3001 aaatgtttcc atttgctgta caatttctga gagaagagat tgtccgtgcg gaagctttag
     3061 gtgctactca aatcaccctt cacccaggcg ctcatgtagg agcaggggca gaagctggga
     3121 ttgctagaat tattgagggg ttgaatgaag ttttgacaaa agaacagacg gcacagatcg
     3181 ctttggagac gatggcaggg aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
     3241 aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgat acatgtcata
     3301 cgaatgatgc cggatataat ataaaagaag atttttcagg tgtaatggaa gagtttgata
     3361 aggtcatcgg attagatcgg cttaaagtac tgcatatcaa tgattctaaa aatccacaag
     3421 gttctcataa agatcggcat gccaatatcg gttttggaac aattggattt gacacattga
     3481 acaaaatcgt ccatgatcca atgtttgaca acatttcgaa aatattagaa accccatatg
     3541 tcggtaaaga taaaaagaaa gcttacgcac catatggaaa agaaatttcc atgttcaaag
     3601 cgcaagcatt cgatcccgaa gtgttccctg agttattagc agaagaagtc acactttaag
     3661 ttttctacct gacagcaaca aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa
     3721 gaaacaaagg agtgcttttt gcattcgttt tcgccatgct ccttttgccc cctcttttta
     3781 aattcagaca aacagggtat actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag
     3841 tagcaaaacg tgattgaaag gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc
     3901 aactaaaaaa gaaacataca tactttattc ttggcatagc cgtattattt acaattattc
     3961 ggttgtattt atatagtaaa gcaatctatg gaacagatat gtcggcgacc tatgatgatc
     4021 agctgctgat tcactatgct caaaatattt tggatggtaa atggctaggg gagtatacga
     4081 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     4141 atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     4201 tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     4261 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     4321 tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg
     4381 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     4441 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     4501 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     4561 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     4621 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     4681 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     4741 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     4801 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     4861 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     4921 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     4981 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     5041 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     5101 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     5161 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     5221 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     5281 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     5341 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     5401 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     5461 taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag
     5521 aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca
     5581 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta
     5641 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     5701 aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt
     5761 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     5821 cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac
     5881 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     5941 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     6001 tggtcatcat cagtaaagga atcatcgaaa atggcaacgc aaaagccaag aacacctggg
     6061 attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa
     6121 acaaaacaca aatcaataca ggaatcacgg aaagcaatcg tgtgaccagt cttcttagcc
     6181 aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag
     6241 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac
     6301 cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt
     6361 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     6421 tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag
     6481 tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt
     6541 gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     6601 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     6661 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     6721 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
     6781 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
     6841 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
     6901 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
     6961 tccgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
     7021 tatactgtaa aagcatagca attagactag acatcaaaat aactgacatc aataaatatt
     7081 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
     7141 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt tttccctttg
     7201 gtacatccac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
     7261 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
     7321 tctacgatta cattctacac ctcagaaaga aaaaattaaa gaaaaagatt cggtatgcct
     7381 aatttttttt cgtggtgtgt cttgctggtt aatgaaagaa attttgttct tctctaaaaa
     7441 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
     7501 aaaataaatc attgagagca attcggaaat agaaaggaga gatttatatg gtatcaacag
     7561 gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc
     7621 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
     7681 tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga
     7741 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaaagactt tttgcgggct
     7801 gattttttca gtccgttttt tatttcgtaa tttgttagac tattcagact attcagcaaa
     7861 aaagacaagc acagacaatc ctatgctctt gataaagaca aaggaggagt aaaatgtcga
     7921 tttttagaaa attaggttgg ttttttcgtc aggaaaagaa acattattta attgggattt
     7981 tctccctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca
     8041 ttgatgagat cgccgataag aatttatcaa taaagatcat ttcctggtgg atcttattat
     8101 tgatccttgc agctttctta caatatcttt ttcgttatat ctggcggacg aatatttggg
     8161 gaagtgcagc tcggttggag agagaacttc ggcagcagct attcaaacat tttacagaaa
     8221 tggacaatct cttttaccaa aaatatcgga ctggagattt gatggctcat gcaacgaatg
     8281 atttgaatgc aatccagaat gtagcaggag ctggaatctt gacctttgca gattcagtga
     8341 ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga
     8401 ttgcgctgat tcctctgcct ttgctagccg ttacttcacg agtgctgggg tcaaaactcc
     8461 acgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa
     8521 gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctcgcggatt
     8581 ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct
     8641 tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag
     8701 gtggaaagta tgtcttggaa ggcacgatct caatcgggca gatggtctca tttattagct
     8761 atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa
     8821 gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg catatcgtgg
     8881 aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt
     8941 ttaactatcc gaaagaagaa tcaccagcat tagaacaaat caaatttgcg atccaagaag
     9001 gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacaatc atcaaattac
     9061 tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag
     9121 aatattcatt gaatgcttta ttaggatcaa tcggttatgt gcctcaggat cacttcctgt
     9181 tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag
     9241 tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaattg ccaaaaggtt
     9301 atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa cagaggattt
     9361 cgattgctcg agcattgatc aaaaatcctg aattgctgat cttagatgat gcactttctg
     9421 cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga
     9481 aaacaaccat cattgcagct caccgtttaa gcagcgtgat gcatgcaaaa gaaatcattg
     9541 ttgtcgatca agggaaaatc attgaacgag gaacccacca cgagctgctt gcattaggtg
     9601 gctggtatga acgaatgtgg gaaaaacagc aattagaagc gaagatagaa ggaggcgagg
     9661 cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa
     9721 gaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc
     9781 tatcttattt gcgtttgccc tgtctgtgat caatgtccta ttgccaagga tcatccaaac
     9841 ttttatggat gatcatctgg ctaaacagtc agctacgaca caagttattt tgttttttgc
     9901 tggtgtgtac ttgtttggag tcatagtcaa aagtatcatt tggtttttcc aatggtattt
     9961 gtattccatg gcttcattga agacctatca gtatgttcgg gtaagattat ttgaaaagct
    10021 gcatacacta ggtatgcgtt attttgacca gacaccggca ggatcgaccg tttctcgagt
    10081 gacgaatgac acagaaacgc tatttgaatt ttggtatgtc tttttgatgg tattgactgg
    10141 gatctttgca gtcgtttctt ctttcgttgc catgtttcaa atcaatggag aaattgcctt
    10201 gtggactttg gtgttcttgc cgattttagg aatcgtcatc tggtattacc agaaattcag
    10261 ttctcgtatt taccgaaata tgcgggaaaa attaagtcag ctgaacacga aattgaatga
    10321 atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga
    10381 atttgaacaa gtgaacaacg agtatttaga cacgcgctat gcgatgatca aaacgaattc
    10441 gattctgtta agtccaatca tcaattttct ctatgcattg gcgattgcgt tggcgttgac
    10501 tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac
    10561 gacctatgtc caggaatttt ttaatccaat gtctcaaatg atggactttt tgagcacctt
    10621 tacagatggg atggttgccg gtagtcgtat tttgaaaatc atggatacag aagaatatgc
    10681 accgcaacaa aatcctggag cagatcaagc gattacttca ggtaagatcg agtttcgcaa
    10741 tgtaagcttt tcttatgatg gagagcacaa tgtattgaac aatatctctt ttgtcgctaa
    10801 tccgggtgag accgttgcat tagtggggca caccggaagc ggaaaaagct cgatcatcaa
    10861 cgtattgatg cggttttacg aattttatga aggccaaatc ttgattgatg atcatgacat
    10921 tcgagaatat ccaatggaag agttacgtaa gaaaatggga ttggtcttgc aagatgcctt
    10981 cttgttttat ggagatattg caggaaatat ccggatgatg aatccagcta tcacagacga
    11041 acagattcgc catgcagctg agtttgttca agcaaacaag ttcatcgatg aattaccaaa
    11101 aggatatcac tcaaaagtga tcgaacacgg tgccagctat tctagtggac aacgacagct
    11161 gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata ctagtattgg atgaagcaac
    11221 agcaaatatc gatactgaaa ccgaaggatt gatccaagaa ggattggcaa aaatgcgaca
    11281 aggacggaca acgatagcga ttgcccatcg gctgtccacg attcgggatg cagaattgat
    11341 tttagtttta gacaaaggtc atatagtaga gcgtgggaat catgaaacac ttctagcgca
    11401 aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa aagaggttgt
    11461 gacagtagca tagtagcatt ttgccctaag cgctagagtg aaaataaaac agagcttcgc
    11521 agattcttca tctgtgagcg ttagtggcgt agtcacccga tttaaaaaag acgacgacag
    11581 ctagtcaaaa gcggctaata accactaaga aagcgttgac aaaagcgttt gctttcgata
    11641 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag
    11701 tctcccatgg ttccatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta
    11761 tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa
    11821 taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa
    11881 agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg
    11941 acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa
    12001 tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg
    12061 tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat
    12121 ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg
    12181 ggaaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagatgaa
    12241 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca acaagacaat
    12301 acctacctgt tcactataac aatacaggat attctaaata tcgtccgcta ttactataag
    12361 attacatttg atgaagattc agtccatttt tttcgattcg tcacacactt acgttttttt
    12421 ctgacacgcg tcctagatgg agaaaaatca gaagtgggag aagtttctga ggaattgttt
    12481 caggttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
    12541 ttagccgaga cgttcaacta ccagatgtcc acggacgaaa aaatgtattt aacgatccat
    12601 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaaggagg ataaattaac
    12661 aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg
    12721 gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc
    12781 gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa
    12841 aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg
    12901 cgagcgggag ggcagtatca agttgtcatc gggaatcatg tgccagtcgt gtttgaagag
    12961 gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg
    13021 tttgatatgc ttattgatgg aatatcgggc tgtttccaac cattccttgc tatcctagct
    13081 gctgacggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc
    13141 ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct
    13201 gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg
    13261 cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa
    13321 gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta
    13381 ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt
    13441 gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg
    13501 aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt
    13561 ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca
    13621 ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt
    13681 ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa
    13741 aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc
    13801 tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt
    13861 attgtgggga ttacagagcc agctatttat gggtttacat tgccacgtaa aaaaatattt
    13921 atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact
    13981 tcttggaatc aaggtggact aggcatcttt acaattccca actatattag accagatggt
    14041 gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt
    14101 gcgctgactt ttttcttttg gaaggaggaa gcagatgaaa cagatgatag acagaaaaga
    14161 tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact gaatgcagca
    14221 aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa
    14281 ggagaagtac gtgccccttt tgacggaaca gtcatgtccc tgtttccgac gaaacatgca
    14341 atcgggatgg tttctgaagc aggtctggaa ttgctgattc atgtcggatt ggatactgtt
    14401 cagttggaag ggaaatattt tgaaagcttg gtgcagcaag gagaaagagt gaaacaaggc
    14461 gatctgttgc ttcgcttcga caaaaagaaa atcgaagagg aaggatacag tttagaaaca
    14521 cctgtgattg tcatcaatta catggattat ttggatattt tggaaaatca aggggaaacg
    14581 gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg aaagaatgga
    14641 ctaggcttaa ctattctttt tagtccactt ctttattttc aaaattgaaa ggctctttca
    14701 tgatggctga gctgataggg cgtttatcaa atttttgtcc tacagaaatt agatggacta
    14761 aattctgttg ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa
    14821 tcgatgtatt gatcgtaaaa ttaccaattt tcgaatacag tcgttcgcgt gtcgtaagtc
    14881 gcaaagcggc tgtactcatt gaagatagcg ttgaatcacc gatactggtc aaagctaaaa
    14941 ttggcacatg attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg
    15001 tataagaaat cagaatccca cacatatcac ttgtgcaatt gtacgcttca tagaacgatt
    15061 caccaaaagt ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat
    15121 cttgagaaat caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca
    15181 ttagttgttt ggctttttgc agttggtcat gatgaatgag agaaagtgta tcttgaatcg
    15241 tcatctgttc taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa
    15301 aaggaagatt cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa
    15361 aagcattacg aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg
    15421 tggacgggtg gacataacat cgctcagcaa tctcttggat ggtcatttcg ccaagtgctg
    15481 gagaatcgct taagagaaac tgaatcactt cattttctga aggagaaaag gagatttctt
    15541 tgattttttc acgtaataac atgtagatac cttcttcttg tttttttacg taaattataa
    15601 cttttttgaa aatgatttgt aaacggattt atgttatata gcttaataaa gacatttttt
    15661 gacaagatat ttacaaaaat ccagaagaaa acactaactt ttgtaaatgt tactggaaag
    15721 aaacgaattt ctattgtttg ttttgtttac aaaaaagata ataaaagtgt aaaaagtaaa
    15781 ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca
    15841 cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct
    15901 ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgtcagtcag atgtctcagc
    15961 gtatgtggga tgcttctccg tatgaagtca aaatctatgc aactctggat cgggaagaag
    16021 cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa
    16081 tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg
    16141 gcgggatatt caaagctttt cgtaccgttc cagtggtttt ggcaatcgtt gaagacatga
    16201 agcgtttatg tccaaatgct tggctgatta atttcgcgaa cccaagcggg atggtgacag
    16261 aagcagttat cagatatggc aaatgggata aagtgatcgg attatgcaat gtgccagtaa
    16321 tggcgatgat gacagaacca acaatgcttg gcaagaaaaa agaagagctg atctacaagt
    16381 ttgccggtct aaatcacttc cattggcaca aagtagcaga taataaagga aatgaccgca
    16441 cggcagaatt gattgatcgt ttatacgatg aagacaatgg attgccgaaa aatatctttg
    16501 acatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc
    16561 gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg
    16621 gtacaagagc gcagcaagtg aaagaaacag aagcagagct gtttgaatta tacaaagatc
    16681 cagaattgga tcataaacct gaacaattgc aaaaacgagg tggtgcttat tattcagatg
    16741 ctgcctgtga aacgattgct tcgatctatg gaaataaaga aacacagatc gtggtatcta
    16801 caaagaatga tggagctgtt cctgatctgc cggcagattg tgtagtagaa gtcactgcct
    16861 atgttggcgc acaaggcgca cgaaatgtag cgtttggttc ccttcctgct gctgaaagag
    16921 gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagcc gtgactggtg
    16981 attatggaac agctttgcaa gcgtttacga tcaatccgct tatacgatca ggaaagacag
    17041 caaaacggat catggatgag ttgtttattg cacatcagca gtatttaccg caattcaagg
    17101 aaacaatcag ccggttaaaa gcagaaggaa tcgaagtcca agatgaactg gcaagagaat
    17161 tatcaagcaa tacacatgta atggaggaag tatgatggga ttcagaaaag attttttatg
    17221 gggcggagct acagcagcca atcaatgcga gggcggttat gatgaaggcg gacgtgggtt
    17281 agcgaacgta gatgttgtgc caatcggaga agaccgtttt cctgtcatta ctggaaaaaa
    17341 gaaaatgttt gaatttgatg aaaatcattt ttatccagca aaaattgcga tcgatatgta
    17401 tcatcattat aaggaagata ttgctttatt tgcagaaatg gggttcaaga cttatcggat
    17461 gtccattgct tggagtcgta tttttccaat gggcgatgaa aaagagccaa atgaatctgg
    17521 gttgaaattt tatgaggatg tctttaaaga atgtcaaaag tacgggatcg agccgcttgt
    17581 gacgattaca cattttgatt gtccgatgca tttgatcaaa gaatatggtg cgtggcgttc
    17641 cagagaactc gttggttttt atgaaaatct gtgccgagtc atcttcaatc gctataaagg
    17701 acttgtcaaa tattggttga cattcaatga gatcaatatg atccttcatg caccattcat
    17761 gggcgcaggg ctgtattttg aagaaggaga aaatgaagaa caaatcaaat atcaagcggc
    17821 acatcatgag ttggttgcaa gtgccatcgc aacgaagatc gctcatgaag tagatccgga
    17881 gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga
    17941 agatgtttgg gcttctagaa agtcagatcg agaaaattat ttcttcattg atgtccagtc
    18001 acgaggagaa tatcccgctt atgcattgaa agaaatggaa cgaaatggca ttcagatcaa
    18061 aatggaacct ggggatgccg cattgctgaa agaacatact gttgatttta tctccttttc
    18121 ttattattct tctcgagttt cgactactga caacgaactc ttggaacaga ctgcaggaaa
    18181 tatctttgct tctgtcaaaa atccatatct ggaagcaagt gaatggggtt ggcaaattga
    18241 tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccactatt
    18301 tattgtagaa aacggcttag gtgcagtcga tacgcccgat gagaatggtt atgtggaaga
    18361 tgattatcga atcgactatc tggcagcaca tatccaggca atgaaagatg cagtagaatt
    18421 agatggagtc gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg
    18481 tacaggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgaca acgaaggaaa
    18541 tggcacatta gcccgttcga agaagaaatc attcaactgg tataaaaaag taattgattc
    18601 aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag
    18661 cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt
    18721 tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga
    18781 tgtaatcgtt attggcgcag ggcctgctgg gatgactgct gctttatatg cttcacgttc
    18841 aaacttatcc gtattgatga tcgaacaagg cgctcctggc ggccaaatga acaatacggc
    18901 agaaatcgaa aactatcctg gtttcgattc aatcatgggt cctgatctag cttataagat
    18961 gtatgaaggt gtttcaagat ttgggacaga aaatgcttat ggaatcgtgc aagatatcaa
    19021 ggatcacggc agccacaaag aagttatttg tgaagataaa acttacgaag gaaaaacagt
    19081 cattatcgca acaggctgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc
    19141 tggacgtggc gtttcttact gtgcagtttg tgatggtgca tttttccgta acaaacgtct
    19201 agtagtgatc ggaggaggag actcagcagt agaagaagcc atctatttga cacaatttgc
    19261 ttctgaagta gtcatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga
    19321 ccgtgcattt gcgaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt
    19381 tggaaatgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaactg gcaatgagtt
    19441 tgatttggca gcagacggag tattcatcta tgtaggattg gatccattaa ctgcaccatt
    19501 tagagaagct gggttgacga acgaagccgg ctggattcca acagacaacg aaatgaagac
    19561 gaatatacca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac
    19621 tactgctgtt ggtgatggtg ggattgctgg acaacaagtg tataaatact tggaatcttt
    19681 agaagaaaca actgcataag tttaaaaaga gtgaaagaac ggtttcaatt gttcttttac
    19741 tctttttttt gcgtgaaatc tactgaatcc gatttatgcg aatacttgta accgtgcttg
    19801 ataaaagaat cttttcttga tttaagcgtt ttttggtcct catactttca tatctaataa
    19861 gtgaatgcta tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg
    19921 gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa
    19981 ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa
    20041 ttcggtactg caggcatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat
    20101 acggtaagac aagcaacaga aggacttgca cgtttcatgg atacacaagg tgaagaaaca
    20161 aaacgccgag gtgtcgctat cgcttatgac tcacgccatc aatcaccaga atttgccatg
    20221 gaagcagcca aaacattagc acatcacaat atcccttctt atgtattcga aagtttacgt
    20281 ccaacaccag agctttcttt tgcagtccgt catttgaaga cgtttgcggg aatcatggta
    20341 actgcatccc acaatccagc agcttacaac ggatataaag tctacggaga agatggggga
    20401 caaatgcctc ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg
    20461 ttaaaagttg aagtattatc tgacgacgaa gctgaacata gcggattgat cacgatcatc
    20521 ggagaagaag tggatgccgc ttatctagaa gaaatcaaag cagtaacgat tgatcatgaa
    20581 ttggtggaaa caatgggtaa agatttgaag cttgtgtaca caccacttca cggaacagga
    20641 aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttcca
    20701 gaacaagctg tcgcagaccc ggatttcact acagtcaaat caccgaatcc agaagaacac
    20761 tctgctttcg agtatgcgat gcgacttggc gagaaagaag gcgctgactt actgattgca
    20821 acggatcctg atgcagaccg tttaggcgca gccgttcgtt tgccaaatgg ctcctatgaa
    20881 gtattgacag gaaaccaaat cggagccctg tttgctcaat atattttgga agcacaccaa
    20941 tcgcgaggca ctttacctga aaatgcagcg gtattgaaat caatcgtttc tagcgaattg
    21001 cctacagcaa ttgcggaaca ctatggggct accatgttca atgtattgac aggattcaaa
    21061 ttcattgctg aaaaaattca acaatttgaa gaagatcatt cccatacttt tatgttcgga
    21121 ttcgaagaaa gttacggtta tttagttaaa cctttcgttc gcgacaaaga cgcgattcaa
    21181 gcattgctct tattagcaga agtagcagct tactataaaa aacaaggcaa aacattgtat
    21241 gacggcttgc aaacgatttt tgaaacatat ggctactacg cagaaaaaac gatttctgtg
    21301 acaatgagcg gacaagaagg ctctgcaaaa atctctgctt tgatggaaaa attccgtgaa
    21361 gaagcaccga cagaatttgc cggagcaaaa gtcatccaaa cagaagactt caaagagttg
    21421 acgagaacag accgtgaagg aaacgtagaa aaactaacga caccgccatc tgacgtattg
    21481 aagtatacgc tggaagaaaa tggctggatc gctatccgtc cttcaggaac tgagccgaaa
    21541 atcaaatttt atatcggtgt aaaaggaaac tcttcagaag acgcagctga aaaagtagcc
    21601 gcttatgaag cgattatcaa aaaaatgaca gaagaataaa aaaacttgag actgatggaa
    21661 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
    21721 acgcaaaaaa caaaagtaga gaaagtcagc caactattca aaatgttgag tgaccccact
    21781 cgtctcaaaa ttctgctgta tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
    21841 gcagtagaaa tggaacaatc tgctgtatcg catcaactgc gtttattaag agaaaaccat
    21901 gtcgtcaagt cccatcgtga gggaaaagcg attttgtata gtcttgatga ttctcacgta
    21961 ctggatattt tgaaccagac gctcaaacat gtggaacatc aataacgata ggtgcaaata
    22021 gagacaaact ggactgtatc aacgaatctt cttcgctgac acagtccagt ttttttaaga
    22081 aaaagacaag aaaaactgtt agtctgtttc ttcggtggtt tcagttccat aataactatg
    22141 atcttctagg tctaaagcat tcaaaataag tgaagctgtt tcttcgatag ataaagaagc
    22201 gacattgatt actacgcagc ccaatttttc ataaagatca ttcgcaaaat cgagttcagc
    22261 tcgtattttt tcgatatcgg aataagcagt atcaggattt aatccgtaag agcgcatccg
    22321 ttctttacgg atattgttta aaatatctgg gttattcgtc agtcccacga tttttttagg
    22381 atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta gattggcaac
    22441 ctttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc tggatacgcc
    22501 tagcaaaacg acatctgctt ctagaaaccc acgaggatct ttcccgtcat cgtattttac
    22561 agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat gaagggcgcc
    22621 aggttgccga atgggcttga taccagttaa gcgctcgatt tctgatatcg gcggagtcat
    22681 cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgcgtcatct cgatcaattc
    22741 ttgattgatc aatgtgtgta ggacaagggc tttttctttt agtgcatctt gcagtgcctg
    22801 catcaatgtt ttctcatctg tcacaaatgt tctgcggaat aaattgaact caacggtagg
    22861 atattgggcc atagtggctt gcgctaattt tgtcgcagtt tctccagcag agtcagagat
    22921 gacaaaaaaa gtaatgattt ctgtagaatc ggaagacatg gataaaacct cacttgcatt
    22981 ttttttatgt ttattatacc atactctaga atgtgtactt cgtaatcatt ttgatttgga
    23041 ggaataagtc atgaaaaaac agacattgat tgaacaatca ctagaaacat tgaaaaataa
    23101 cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga agcgcaatcg
    23161 ctacgtatcc gctaaagaag tttttgacta tatgaatcag gaattcaaag gagtaagtta
    23221 cgatacgatt tatcgaaatc tccatgattt tgcagaaatc gatttactgg aagaaaccga
    23281 tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa ggggaaatcg gccatcacca
    23341 tcactttatc tgtacggtgt gcggaacaac gaaagaaatc catatgtgtc caatgaatta
    23401 tttcgaagaa cagctcggtg gatgtacgat cgaagggcat agattcgaga ttttcggaag
    23461 atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt aaaaaaaagc
    23521 aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca gtcttgtttt
    23581 ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga gggaattaac
    23641 atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg tcgcttcaaa
    23701 cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt ctacgaaaaa
    23761 ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa attctagttc
    23821 tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat gacatgaaaa
    23881 cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc aagtcatcta
    23941 tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa ttaacggtgt
    24001 tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa gcaggacgtg
    24061 acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt cgaaaaatac atgccggaac
    24121 aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcacacaa actggtgctt
    24181 catcaatgaa agaatttggc aaagtcatgg gtgcgatcat gcctaaagtc aaaggaaaag
    24241 cagatggaaa ccaagtaaat gccattgtga aagaactatt acaatcttaa ttttatcatt
    24301 taaactgcgg acaatcattg tccgcagttt ttttgtgcgc gtaaaaaaga gaactaaaaa
    24361 atagctagac gaaagaatag gattctttca tctagctggt tttttcctgt ttaatatttc
    24421 ttatgttaaa ttgtgtttga tttggttatt cttctggtag ttcatttgct tcttgtttat
    24481 accctgatag gcgataggcg taataaaaca gtaggctgcc agcgtaacca agtaacgttg
    24541 ggaccatcca gccaagtcct aatgcagcaa aaggaacgtg atgatgataa aattcgagca
    24601 tgcttgctac aactctttgt tgactcacta aagcaggcga atttgccagc atatccaata
    24661 cggcaggaac agcagcaaac aacatcgtta cttgatagac aaatggtgtc ttttggaaaa
    24721 attttgctgt taaggaaagc aagatcaacg caagcgacaa tggatacaaa agcatcaata
    24781 ctggtaaaga ccattggatg atattgtcta aaccagcatt tgcaacgaca aaagagacga
    24841 aagtcgttaa ccgcagccat gtcatataac tgactttagg gaacagttta tggaaatctt
    24901 gcgcaaaaga aacaacgagt cccatagcgg tagtgaagac acctaatgtg accatcactc
    24961 ctaagaagct agcgccgaag ttacctaaat aaaaattagt gatctgagaa agtgcagttc
    25021 cgccattttc tgataaagac atcttattca aactcatcgc accgagaaga acaagtccaa
    25081 aatagatcat gacttctagc gcaacgctca ggctaccagc ttttgccgtt aattctgtga
    25141 cttttttatc tttgaaacca aggtcacgga ctgcatggac aaaagtgaca cttaaggcaa
    25201 gcaaagctac tgcatcaacc gtgttataac cttctaggac accatttgtt aaagcgtttg
    25261 tttgataagt atctgcagca gattgattta gatttcccat tggatggaat aaagaaacga
    25321 caaaaatgat tgctaataat gcaagaaaca aggagtttag atactttccg acgtatttga
    25381 ccaaccgtgt tggatggaca gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata
    25441 aaaacatacc taatgaatta tatttttcag gcaaaaatgg tttagcagcc atttcaaaag
    25501 cagtagcagc tgtccgtggt gtaccgaaaa atggtccgat cgtcaaatga acaaggatta
    25561 agaacaaggc agcataccat tttccgacag gtttagctaa gtcatacaga ccatcacttt
    25621 ttgtcactac tacagctaag atagctagta atgggaaaag agcacctgag atagcaaatc
    25681 ctacgccagc actgacccag tttgcgccag ccatttgacc taagtggaca ggaaagatta
    25741 gattacctga cccgaagaac atcccgaaga taagtgaact gaccatcaat agttgtttcc
    25801 atgaataagt ttttgtttct tccataaatt tttcctccaa taattatcat tttgattgac
    25861 gatataaacg ttgcagcaat tagtaatgcc actaatatca gattccatga aaaccaccac
    25921 cttaaaataa aaaaaagccc ttttccactt gggaaaaggg ctctagattc tagaacggta
    25981 ccaccttttt tcgcttaaaa aagcttcttc attcacggac gctttttgat aaagttatcc
    26041 gctagcctga taatggaggc agccacaatg atctacgttt tcgacattga ctcggaaatg
    26101 atgttcgtct attcgattta ttgtcatttc tcacttacat gactcactgg gaataaatcg
    26161 ttgaatagcc tactgttttc ctcgacgctt tgctgtatgt ttatttgttg acgtaataat
    26221 acacctgttt tttttgaaat gcaagaatat tttaactatt ttgttttctt tttttgaaac
    26281 aggaaaaata cctagagaat acttgttgaa tgaaacgaaa tattttgttc tccactttca
    26341 tataacttac attctcagct tctttttatc aaaacgagac ctttagcgaa tggaaacggc
    26401 tattttttaa cgtttcttgg tacgtatggt taagcgactc ttttacaacc ttttcttatt
    26461 tgctatatag tttcttctga gtatggtaaa ataggaaaga atgaatcgaa aaaaagggga
    26521 tgtctatttt tgacggaaga gtctagttct ttagagatta gattgacagc tgctgatgat
    26581 gtcagcatgc ttttaggtgc acacgacaag catataaaac tgatcgagga aacaacggaa
    26641 acaaccatcc atacacgtgg agaaatcatt cagatcattg gtgagcaatc ccagatttcc
    26701 ttggctgcct cagtcattcg gacattacag gaattgatca aacgaggaat ccacgtaagt
    26761 acgccagatg ttgtgacggc attgaaaatg gggcaaaaag gtacattaga ttattttgtt
    26821 gagatgtatg aggaagagat cgtcaaagac cgtaatggaa aacctatacg ggtaaaaaac
    26881 agcggtcaaa agaaatacgt tgaatcggta gccaaacatg acatcgtatt tggtgtaggg
    26941 ccagccggaa cagggaaaac gtttcttgcc gtagtattag caattgctgc cctaaaaaaa
    27001 ggcgaagtgc aaaaaatcat tttgacgcgg cccgctgtgg aggcagggga aaacctaggc
    27061 tttctacccg gggatctgaa agaaaaagtg gatccttatc tgcggcctgt ttatgatgct
    27121 ttataccaaa tttttggatt ggatcatacg aaccgattga tggaacgagg cgtgatcgaa
    27181 atcgcacctt tagcttacat gcgggggcgg acattggatg atgcattcgt tatcttggac
    27241 gaagcacaaa acacgacagt cgctcagatg aaaatgttct tgacccgtct cggttatcaa
    27301 tcaaaaatga ttgtcaatgg ggatacaagt cagatagact tgcctcgtgg gacgacaagt
    27361 ggtcttgttc atgcagaacg aacattaaag caaatcaaaa aaatcgattt tgttaatttt
    27421 gaagcaagtg acgttgttcg gcaccctgta gttgccgaaa tcatcaaagc ttatgaaaag
    27481 gcggatcttc atcaagaata agctttgctt aggaggataa gcacatgttg aataaaacac
    27541 tgcagaaact acaagaaaaa ctcggaaagg cagcattgcc tttccttctg ttcttgtttt
    27601 ttatcattgt ctgcggcgtt acatttagta gtgtcaaaca aaaggccatt gatttcagag
    27661 aaggccaagt agcagaagag agtatccgtg cgaataaaac gattgagaac acggaagaaa
    27721 ccgaacaaag aagaaaactg gcagcagaag cagtgactcc agaatatact tatcaaaaag
    27781 atctggcaga tgaccagaac aatcggatca agcaattatt cgaattgatc gataagacga
    27841 atgataccat caacaaatct tataatggca aggtagataa ggcgaaggaa gatgaaaata
    27901 tcccgaaacc tactacagaa gagcggattg ctacactgaa gaaaaatttt gaaaacgtga
    27961 atgcagaaaa cgttgcgttt taccaaaaac tgccgaacaa tttttattcg acgatatttg
    28021 aaatgactga aacggaaatt cacacagtta gggatgaaag tttgaagctc attgacgaac
    28081 agatgagtaa acaagttcgt gaaagcgaat tggaagcttt taaacaagaa gcagaagatc
    28141 agattcaata tttgaatgtc acaacagagc agcaacaaat gattcgctac ttagtggatc
    28201 aaggcatcgt agttaatgat gtattgaatg agaaaaagac agaagaatta aaacaatctg
    28261 cacgagaagc tgtacaacca gtgatgatct tccaaggaga aattattgtt cgcgaaggaa
    28321 atcaaattga tgcttcagcg atgaaaaaat tggaattact aggattgacg aatcaaacga
    28381 cttctatttt cccactcatt gccatggttt tagctgtttt gcttcaaatc ggtgtactga
    28441 tttataattc acttcaattc caaaatgcag gagaaagaac aaaatacgta cttttctacg
    28501 tgacggcaat gtcagtaagc gtcgtactaa tgaaattttt ccaactcttc caaacagaac
    28561 aagctgctta tattccgctg ttttaccccg cggcttttgc tccacttgtt ttaagctttt
    28621 tcttgaatcg aagagcaggg atcatggctg gtatttttca agcagtatcc gcacttttca
    28681 ttttttatga ttcgattggc acgaactttc tgacagttat tttgatgtcc tatgtatttt
    28741 ctggtctcat ggcaacgatc gtcagacgca aacggatctc agaacaaggg ttatccgctg
    28801 ctatgtgggt cattattttt cctgtgatga tggatgtcat tttgattatc taccaaggaa
    28861 tgagctttgg ggatgcaacg acttggttaa cgctagtctg cggattcaca ggtgcactgt
    28921 tttcattctt gctgacgata ggtctgcacc cctatattga attgatggtc aatgatgata
    28981 gtgtgatcgt attaaatgaa ctcagcaatc ccaatcatcc tttattgaaa cagttgctgg
    29041 aagaagcacc aggtacgtat caccatagca tgatggtagc caatctaagt gctaatgctg
    29101 tagcagaaat cggtggacgt tctttactaa ctcgagtagc ttgctattat catgatatcg
    29161 gcaagatcaa acacgccagc tttttcgttg agaacttgcc ttcaggagcg gaaaatccgc
    29221 acaatttctt attgccagaa gacagtaaac atattatctt tggacatgtg acagatggtg
    29281 ccaaaatatt ggaagattat aatatgccgc aaatggtgat cgatatttgt agacagcatc
    29341 atggaaccac gctgatgcgt tatttctacg ttaaggcaaa ggaacgcaac ccggaagtta
    29401 cggaagagca attccgttat cctggaccaa aacctcaaag ccgtgaagca ggaatcgtca
    29461 atatagctga cagttgtgaa gcggcagtac gggcaatgga ccatccaacg agcgaaaaaa
    29521 tcgaaagttt tgtccataat cttattgaag agcgtatttc cgatggacaa ttggacgaca
    29581 gtggattgac gttaaaagaa atccgtaaag tagaaaaatc attgatcagc ggtttaagca
    29641 gtaccttcca ttctcggatc aaatatccta aaatgaaatc ggaagttgag aagataaaag
    29701 aagaacaaga agaatagcaa gaagaataga aagaagcgat gtataatgga tattaccttc
    29761 atggatgaaa caaaccaagt ctcagaagag aaaatgaaag aaatcgacga cttgctgcaa
    29821 tttgcagcat catatttaga gttgccagaa gaaacagaaa tgtctgttac tttcatggac
    29881 aatgcagcaa tccaagtaat caatcgtgat taccgtggaa aagatgcacc gacagacgtc
    29941 atcagttttg ccttggaaga agagggcgaa gacgaaatcc cagtgatttt tgaagatgga
    30001 gagaatccat tgcctcgtgt actgggagac atcatgatct ctactgaacg tgctgcagag
    30061 caagcagaag cttacggcca ttcgtatgat cgggaacttg gctttttagc ggttcatgga
    30121 ttcttgcata tcaacgggta tgaccatatg acaccagagg atgagaaaga aatgtttggt
    30181 ttacagaaag agatcttaga tgcctatgga cttaaaagat aaaaacgtag agaaaaacaa
    30241 acatttcctg atgtccgtgg aatttgccgt ccaaggagtc aaaactgttt ttgaggaaga
    30301 acgcaacatg aggaaacatg ttgcgttcgg aatcctcgct ttgtttatgg gcgtgatttt
    30361 ccaacttgat cggatagaat ggttatggct gcttttagct gtttttctcg tatggatagt
    30421 agagatcatc aatactgttt ttgaaaatgt cgtggacatg tttacggatt ttcattttca
    30481 tccgattggg aaaaaaatca aagatatggc agctggagca gttttagtga ccgcttgttt
    30541 taccgtcatc gttgggttga ttttatttgt ccctaaaata tatcatttgt tgtttaatta
    30601 aaaaggagaa catatatgcc agaacataaa tcagggtttg tcgcaatcgt cggccgccca
    30661 aatgtaggga aatccacatt attgaatcga atcgtgggtc aaaaaattgc catcatgagt
    30721 gacaaggcac agacaactag aaataaaatc caaggagtat acactacacc agaagctcag
    30781 ttgattttta ttgatacacc aggaatccat aaaccaaagc atcgtttagg agattttatg
    30841 gtagaagcag cttacagtgc gttgaaagaa gtggacgcgg tcttatttat gattagtgct
    30901 gatcaaaaac gcggtcgggg agacgatttt attattgaac gtctaaaaaa cgtgcattct
    30961 cctgtttatc tgattatcaa taaaatcgac aaaatccatc cagacgagtt gttgggaatc
    31021 atcgaagatt attcaacgca gatgcctttt gcacaagtga ttccaatctc tgctacagaa
    31081 ggaaacaacg tcgaacgttt gatggatgta ttagtcagtg aaatgccaga gggcccacaa
    31141 tattttccag atgatcaagt gactgatcat cctgaatact tcatcgtttc tgaattgatc
    31201 cgtgagaaag tcttgttttt aacacgagac gaagtgcctc attctgtcgc agtcgttgtt
    31261 gattcgatga agcgaaacga gaatgataaa atccatatcc aggctacgat tattgtcgaa
    31321 agagatagcc aaaaagggat cattatcggc aaaggcggaa aaatgctgaa gcaagtagga
    31381 acgaaagcaa gaaaagatat cgaaaatctt ttaggagaca aagtctttct agaactttgg
    31441 gtcaaagttc aaaaagactg gcgagacaaa aaagtctatc tgcaagactt cggctaccga
    31501 aaagacgaat actgatattc tgagcttctt gtaaattagg tatagaaata aactgaataa
    31561 aaatccgcgt gttgagaaaa gctgcggaag atcgcaacat aaatgatctg gatcggcaga
    31621 atctgctttt ccagatttat tttttaggag ggagaatatg agccagcttg cagaatcaaa
    31681 aggcttgatt ttatctagcc gtaattacaa agaaaaagat aaattagtca aaatctttac
    31741 agaatcttct ggcaaaatga tgttttatat caaagagatc catcggcaaa atcagccctt
    31801 agctccggct ttgctccctt ttactgaagc agtttatatc gggaattttc gaagtgaagg
    31861 tttgtcattt ttaagcagtg tcaaagatgt ccatgcgttt cgacacatcc aagaagacat
    31921 ctttttgagc ggctatggca catatttatt gaatctagta gatgcggcaa tcgaagatca
    31981 tcagtacgac ccgcatttgt tccagtttac gcaacaagca ctgcaacgga tggaccaagg
    32041 agacgatgca gaaatcatca cgaatatttt tgaagtccaa atactgcaac gcttcggaat
    32101 tgcaccaatc tggacacact gcgtcgtatg cggggaaaca aaaggaaaat ttgactattc
    32161 ttccaaatat ggtggtgtga tatgcgaaag acattggtcg atggacgatc accgttatca
    32221 tgcagatcct cgagctgttt atttcgttcg gatgttttca gctatttctt atgataaaat
    32281 tagtggaatc catctgaaag aagaaaccaa acaagcaatc cgtgaactca tcgaccagct
    32341 gtatgatgaa tatgtcggta tccatttgaa aagcaaaaag ttcatcaatc aaatgaagtc
    32401 ttgggcacac gtattaaaga cgtcagagaa agacatgtct gaaaaagaaa aagagacacc
    32461 tgaaaaagaa gaatagtaaa taaaacatac aacaaaaaat caaaaccatt tgacaaacac
    32521 agggagttcc gtctattatt aaccatagat taaatagatc gttcagtgat aggatctatt
    32581 atagaaacaa gaaaactgca aaatgaatac gtacattgac caagatcagt aaaaaacacg
    32641 tggtttagcg agttcgggag agtgggagcc ggacccaaac gatttttgaa gaggcacttg
    32701 cgagtacgat aaatgaagct gccgaatctt tattcggaag tagggtggaa ccgcgaatta
    32761 ttcgtcccta tgtctgtaat cagacatagg gattttttgt cgttttcttt cctgtattct
    32821 tatttgacag caacgcttta aaattgaaag gggcaaaaca gatgagtaaa aaattgaccg
    32881 tccaagatat gattttaaca ttacaaaaat tttggtcatc aaacggctgc atgctgatgc
    32941 aagcttatga cacagaaaaa ggagcaggta cgatgagccc atataccttt ttacgggcaa
    33001 tcggaccaga accatggaat gctgcttacg tagagccatc tcgccgacca gctgatggac
    33061 gttatggtga aaacccaaac cgtttgtatc agcatcatca attccaagtg gtgatgaaac
    33121 cttcacctga gaatattcaa gagctttatt tagaaagctt gcgcctttta ggaatcgatc
    33181 cattagaaca cgacatccgt tttgttgaag acaactggga gaacccatcc atgggctgtg
    33241 ccggtgtggg ttgggaagta tggcttgacg gaatggaaat cacacaattc acgtacttcc
    33301 agcaagttgg tggactgcct tgtaaaccag taacttctga gatcacttat ggattagaac
    33361 gtcttgcttc ttatatccaa gaagttgaaa gtgtctatga tttagaatgg gctgatggcg
    33421 tgaaatatgg cgagatcttc aaacaaccag aatatgaaca ttccaaatat tcttttgaag
    33481 taagcgacca agacttgcta ctaagcaact ttgatcgttt tgaaaaagaa gctaaacgct
    33541 gtatcgatga aaatcttgtt catccggcgt atgattatat cttgaaatgt agccatacat
    33601 tcaacttatt agacgcccgc ggtgcagtat ccgtaacaga gcgtgccggt tatctttctc
    33661 gcatccgcaa catggctcgt gctgtagcca aaatctttgt ggcagaacgc gaaaaattag
    33721 gattcccttt gttgaataaa aacacagcaa atccagaaac cgtgaaggag gctgaataag
    33781 atggcaaaag acttattact tgaaatcggg ctggaagaaa tgcctgccca tgttgtcaca
    33841 ccaagcatga aacaattaga agaaaaaaca gcaaaatttt tagatgaaaa ccaattgact
    33901 tatgatacga tcgaaacatt ttccacaccg cgccgtttag caatcaaagt aacaaatatt
    33961 ccagaacgac aagcggacag tgaagaagaa gtcaaaggac cagccaaaaa aattgctttg
    34021 gacgcagatg gaaactggac gaaagcagcg gaaggatttg tccgcggaca aggtctgacg
    34081 acagacgcta tcacttttag agaattaaaa ggtgtggaat atgtctatgt aacgaaatac
    34141 actcatggta aggaagcaaa agaagtattg acaggcttag caggggtgat caaaagtttg
    34201 actttccctg taacgatgca ttgggcagaa tacgattttg aatatatccg ccctattcac
    34261 tggatcgtgg cattgttaga tgacgaagta atcccattcg aaatcttgga tgtcaaaaca
    34321 ggcagagcct ctcgaggtca tcgtttctta ggcgaagatg tgacatttgc gcatgcaaaa
    34381 gaatatgaag aaaaattgaa tgaacaattc gtcattgcgt ctccgcaaaa aagacaagaa
    34441 atgatcgtta cacaagcaaa cgaattagca gaaaaaaatc actgggttat tgatctagac
    34501 gaagatttac tagaagaagt caacaattta gtcgaatatc caactgtctt ttctggcgac
    34561 ttcgaagaaa aatatctttc tgttcctgaa gaagtactag tcacttctat gaaagaacat
    34621 caacgttatt tcgaggtacg gaatgatcaa ggtatgctgc ttcctcattt tatttctgct
    34681 cgtaatggtg atcgggtaaa attggaagat gtggttcgag gcaatcaaaa agtattgacg
    34741 gctcgtttag aagatgcgga attcttttat aatgaagaca agaaagcttc aatcgaccaa
    34801 tacgttgaaa aactaaaaac tgttactttc catgaaaaaa tcggtagtat ctatgagaaa
    34861 atgcaacggg tacaagtcat tgcccgtatc attggcgaac aagtaggact atctgaggaa
    34921 gaattgatgg atttgcaacg agctgctgaa atctataaat ttgatctagt aaccaacatg
    34981 gtaggggaat ttcctgaact acaagggatc atgggcgaaa aatatgctct tctgaaaggc
    35041 gagaaacctg ctgtagcgac agcgattcgt gagcattaca tgcctatatc cagtgaagga
    35101 gagctacctc agtctacagt aggttctgtt ttagcgattg cagataaatt agatagcatc
    35161 ttttctttct ttgcagttgg gatgattcca agcgggtcaa acgatcctta tgccttacgt
    35221 cgccaagcat acggtgtcgt tcggatcatc gaatcgaaaa aatgggcctt tcctttgtct
    35281 gttctgcaag aaacgattag cgatgttatc agtaaagata cggatcgttt cggcattggt
    35341 ttatcagaag ggcaacaaca agtgatcgac tttatcaaag gccgtctgcg ccaattattg
    35401 acaacgaaga atattcgtca tgatgtcatc gatgctgttt tgaatgcgga acaaaaagat
    35461 ctgacaaaag tatttgcagc agcacaattg ttcaaacaac atcttgcaga tgaagacttc
    35521 aaaccttcaa tggaagcttt gacacgtgtc gtgaacttag cgaaaaaagc gcagcttgaa
    35581 aaacaatcag aagtagaccc tgagctgttc gaaaacgacg cagagaaaga attacacaaa
    35641 gcagtagaag caatgaaagc aagttttgct gaccaaacga tccacgaaaa ctacgaccag
    35701 cttgtcgctc ttcgtccact gatcgaaaat tattttgatc aaacgatggt catggctgat
    35761 gaaccagcag tacgtaagaa ccgactaaca caactaagtc aaatagctgc catggctttg
    35821 tcattagcaa gcttggatca aatcatcaca aaatagtctt ttgtttgaaa aacagagagc
    35881 atgcacgaaa cgtgctgtgt ctctgttttt t
//
LOCUS       DACEMS010000013        35438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEMS010000013 DACEMS010000000
VERSION     DACEMS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.013"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1152
                     /locus_tag="IW735_000601"
     CDS             <1..1152
                     /locus_tag="IW735_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043623.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IW735_000602"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IW735_000602"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1043624.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IW735_000603"
     CDS             2728..3003
                     /locus_tag="IW735_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1043625.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IW735_000604"
     CDS             3009..3776
                     /locus_tag="IW735_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1043626.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IW735_000605"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IW735_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1043627.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IW735_000606"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IW735_000606"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1043628.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IW735_000607"
     CDS             7920..8222
                     /locus_tag="IW735_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1043629.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IW735_000608"
     CDS             8225..9388
                     /locus_tag="IW735_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1043630.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITSPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IW735_000609"
     CDS             9414..12842
                     /locus_tag="IW735_000609"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1043631.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IW735_000610"
     CDS             12863..13987
                     /locus_tag="IW735_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043632.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IW735_000611"
     CDS             13977..14414
                     /locus_tag="IW735_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1043633.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IW735_000612"
     CDS             14398..14736
                     /locus_tag="IW735_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1043634.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IW735_000613"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IW735_000613"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1043635.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IW735_000614"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IW735_000614"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1043636.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IW735_000615"
     CDS             15893..16936
                     /locus_tag="IW735_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1043637.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IW735_000616"
     CDS             17095..17823
                     /locus_tag="IW735_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1043638.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IW735_000617"
     CDS             17939..18184
                     /locus_tag="IW735_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1043639.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IW735_000618"
     CDS             18181..18399
                     /locus_tag="IW735_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1043640.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IW735_000619"
     CDS             18535..19074
                     /locus_tag="IW735_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1043641.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IW735_000620"
     CDS             19067..21340
                     /locus_tag="IW735_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1043642.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IW735_000621"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IW735_000621"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1043643.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IW735_000622"
     CDS             complement(22513..22767)
                     /locus_tag="IW735_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1043644.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IW735_000623"
     CDS             complement(23167..23436)
                     /locus_tag="IW735_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043645.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IW735_000624"
     CDS             complement(23906..24361)
                     /locus_tag="IW735_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1043646.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IW735_000625"
     CDS             complement(24499..26427)
                     /locus_tag="IW735_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1043647.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IVKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IW735_000626"
     CDS             complement(26624..27457)
                     /locus_tag="IW735_000626"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1043648.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IW735_000627"
     CDS             complement(27649..28353)
                     /locus_tag="IW735_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1043649.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IW735_000628"
     CDS             complement(28376..29002)
                     /locus_tag="IW735_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1043650.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IW735_000629"
     CDS             29328..31703
                     /locus_tag="IW735_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1043651.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IW735_000630"
     CDS             complement(31727..32218)
                     /locus_tag="IW735_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1043652.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IW735_000631"
     CDS             32405..33325
                     /locus_tag="IW735_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1043653.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IW735_000632"
     CDS             33479..33823
                     /locus_tag="IW735_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1043654.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IW735_000633"
     CDS             complement(34013..34513)
                     /locus_tag="IW735_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1043655.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IW735_000634"
     tRNA            35041..35124
                     /locus_tag="IW735_000634"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11775 a         6171 c         7297 g        10195 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg tccccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgacgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcggttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DACEMS010000014        34619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEMS010000014 DACEMS010000000
VERSION     DACEMS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.014"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(68..916)
                     /locus_tag="IW735_000635"
     CDS             complement(68..916)
                     /locus_tag="IW735_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1043656.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1140..3080)
                     /gene="thrS"
                     /locus_tag="IW735_000636"
     CDS             complement(1140..3080)
                     /gene="thrS"
                     /locus_tag="IW735_000636"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1043657.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3417..3596)
                     /locus_tag="IW735_000637"
     CDS             complement(3417..3596)
                     /locus_tag="IW735_000637"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1043658.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3643..5466)
                     /gene="cadA"
                     /locus_tag="IW735_000638"
     CDS             complement(3643..5466)
                     /gene="cadA"
                     /locus_tag="IW735_000638"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1043659.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5744..8023
                     /locus_tag="IW735_000639"
     CDS             5744..8023
                     /locus_tag="IW735_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1043660.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8116..8763)
                     /locus_tag="IW735_000640"
     CDS             complement(8116..8763)
                     /locus_tag="IW735_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043661.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8942..9661)
                     /locus_tag="IW735_000641"
     CDS             complement(8942..9661)
                     /locus_tag="IW735_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1043662.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9792..10943)
                     /locus_tag="IW735_000642"
     CDS             complement(9792..10943)
                     /locus_tag="IW735_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1043663.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11160..11900)
                     /locus_tag="IW735_000643"
     CDS             complement(11160..11900)
                     /locus_tag="IW735_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1043664.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11904..12227)
                     /gene="rsfS"
                     /locus_tag="IW735_000644"
     CDS             complement(11904..12227)
                     /gene="rsfS"
                     /locus_tag="IW735_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1043665.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12284..12877)
                     /locus_tag="IW735_000645"
     CDS             complement(12284..12877)
                     /locus_tag="IW735_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1043666.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12861..13508)
                     /locus_tag="IW735_000646"
     CDS             complement(12861..13508)
                     /locus_tag="IW735_000646"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1043667.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13521..13832)
                     /gene="yhbY"
                     /locus_tag="IW735_000647"
     CDS             complement(13521..13832)
                     /gene="yhbY"
                     /locus_tag="IW735_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1043668.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13848..14957)
                     /gene="yqeH"
                     /locus_tag="IW735_000648"
     CDS             complement(13848..14957)
                     /gene="yqeH"
                     /locus_tag="IW735_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1043669.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14954..15481)
                     /locus_tag="IW735_000649"
     CDS             complement(14954..15481)
                     /locus_tag="IW735_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1043670.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15661..16446)
                     /locus_tag="IW735_000650"
     CDS             complement(15661..16446)
                     /locus_tag="IW735_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1043671.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16439..17308)
                     /locus_tag="IW735_000651"
     CDS             complement(16439..17308)
                     /locus_tag="IW735_000651"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1043672.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17309..18688)
                     /locus_tag="IW735_000652"
     CDS             complement(17309..18688)
                     /locus_tag="IW735_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1043673.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18696..19115)
                     /gene="fabZ"
                     /locus_tag="IW735_000653"
     CDS             complement(18696..19115)
                     /gene="fabZ"
                     /locus_tag="IW735_000653"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1043674.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19140..19616)
                     /gene="accB"
                     /locus_tag="IW735_000654"
     CDS             complement(19140..19616)
                     /gene="accB"
                     /locus_tag="IW735_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1043675.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19619..20854)
                     /gene="fabF"
                     /locus_tag="IW735_000655"
     CDS             complement(19619..20854)
                     /gene="fabF"
                     /locus_tag="IW735_000655"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1043676.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20873..21610)
                     /gene="fabG"
                     /locus_tag="IW735_000656"
     CDS             complement(20873..21610)
                     /gene="fabG"
                     /locus_tag="IW735_000656"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1043677.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21622..22539)
                     /gene="fabD"
                     /locus_tag="IW735_000657"
     CDS             complement(21622..22539)
                     /gene="fabD"
                     /locus_tag="IW735_000657"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1043678.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22646..22870)
                     /locus_tag="IW735_000658"
     CDS             complement(22646..22870)
                     /locus_tag="IW735_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1043679.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22923..23885)
                     /locus_tag="IW735_000659"
     CDS             complement(22923..23885)
                     /locus_tag="IW735_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1043680.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23895..24335)
                     /locus_tag="IW735_000660"
     CDS             complement(23895..24335)
                     /locus_tag="IW735_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1043681.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24641..25606
                     /locus_tag="IW735_000661"
     CDS             24641..25606
                     /locus_tag="IW735_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1043682.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25663..25893)
                     /locus_tag="IW735_000662"
     CDS             complement(25663..25893)
                     /locus_tag="IW735_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1043683.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25979..26866)
                     /locus_tag="IW735_000663"
     CDS             complement(25979..26866)
                     /locus_tag="IW735_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1043684.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26955..28178)
                     /locus_tag="IW735_000664"
     CDS             complement(26955..28178)
                     /locus_tag="IW735_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1043685.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28195..29274)
                     /locus_tag="IW735_000665"
     CDS             complement(28195..29274)
                     /locus_tag="IW735_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1043686.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29405..31132)
                     /gene="ptsP"
                     /locus_tag="IW735_000666"
     CDS             complement(29405..31132)
                     /gene="ptsP"
                     /locus_tag="IW735_000666"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1043687.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31132..31398)
                     /locus_tag="IW735_000667"
     CDS             complement(31132..31398)
                     /locus_tag="IW735_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1043688.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31877..34111
                     /locus_tag="IW735_000668"
     CDS             31877..34111
                     /locus_tag="IW735_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1043689.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34259..34579
                     /locus_tag="IW735_000669"
     CDS             34259..34579
                     /locus_tag="IW735_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1043690.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9779 a         7486 c         6041 g        11313 t
ORIGIN      
        1 gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat aaaaaatagt
       61 aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt tttttcactt
      121 tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg atcgtagcaa
      181 attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt ggtctagatg
      241 tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc gtcaacgcaa
      301 tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct gcacttctac
      361 ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt tgcgggagca
      421 agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat ttttgcagaa
      481 tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc tctgccgatt
      541 ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact gtttctttta
      601 tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac tcatttgctt
      661 ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc gtgattttga
      721 agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc ggcgcactca
      781 acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca atattaccga
      841 taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata ctttcaatac
      901 gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta agtataccat
      961 atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat ccgcccaaca
     1021 gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt tgaggaatac
     1081 ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa atttacctat
     1141 tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa gatattcaaa
     1201 tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc atcttccaat
     1261 tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc acgtatctta
     1321 tagcccattt tttcattacg atcatcaact tctacacgca accccttcat ttgcaaacgt
     1381 tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat gattgttgct
     1441 tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa ataagcgaca
     1501 aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg cgtattttcc
     1561 ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc taattgtata
     1621 gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag ttttggtccg
     1681 taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc catagcggct
     1741 ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt atctgtattg
     1801 tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc agcatatact
     1861 gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg acgaacaaat
     1921 gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa agcacctgat
     1981 ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc acgataagaa
     2041 tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg aagaacaagc
     2101 atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg atcccagtgt
     2161 ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg ttggtaacct
     2221 agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt tgcccctttt
     2281 ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa gtcaagttct
     2341 tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat atattctttc
     2401 aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg attgtttgag
     2461 tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat acggccagtg
     2521 gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata agcagtaatc
     2581 acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact tgcaaaaatt
     2641 tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc tttgacgatt
     2701 ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt ttgtccatta
     2761 tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg gatatttgga
     2821 tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac taagccaagt
     2881 gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat tggacgagtc
     2941 aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa gctttttgaa
     3001 atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac tgcaccatct
     3061 ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg atcagatcat
     3121 caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg acgaaaagtt
     3181 cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa cggtccgacc
     3241 gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc tggaaaagtt
     3301 tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt tctcatcaat
     3361 caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa ctaaacttat
     3421 tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat gatgacatgg
     3481 atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc tttcatcata
     3541 ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa tggcataatg
     3601 ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta tgtttgtctt
     3661 gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac tcaaaataga
     3721 gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta gtcccgtact
     3781 tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact gtttcgctat
     3841 tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat catctttcaa
     3901 gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca ttgcaatacc
     3961 gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca tcgcaacagg
     4021 tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca agctacgggc
     4081 atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag atccatcccc
     4141 ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg tctttgctgc
     4201 ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat caattgttac
     4261 atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg cacgtccaat
     4321 ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt ctttcagttg
     4381 actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag ctaagatatg
     4441 gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt ttattccatt
     4501 gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg ttttatcaaa
     4561 agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta caacgatccc
     4621 gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta gaatcagtgg
     4681 acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg agaaccacca
     4741 agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac gatcagctaa
     4801 acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa tcatttgata
     4861 ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc catttaaaga
     4921 ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac ctgtcaaaga
     4981 agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta caagttcgcc
     5041 aggcttgact agtaactggt catcaatatt tatttcatcg attactatat cctgcaattt
     5101 tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact gcagttcttt
     5161 acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca tgatcaatat
     5221 catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg taatcgctaa
     5281 tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa acatcaagaa
     5341 ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct ggtgaaaaac
     5401 aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt ttttgaaatg
     5461 tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa atttccagct
     5521 gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata ggccttaatt
     5581 gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac aaaaatattc
     5641 agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa aaactatgct
     5701 atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg agaaacaact
     5761 agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa atatgctgga
     5821 tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca cagctaatca
     5881 caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat atcgccaaca
     5941 tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac gcctgcaaac
     6001 gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag aagaccgtga
     6061 aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag aaacaatcgt
     6121 tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg gtcccgcatt
     6181 ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac aatttaaaat
     6241 ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg atgacttttt
     6301 gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct ctacgataca
     6361 aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga ttgaaggaat
     6421 cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt tgtatcataa
     6481 tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt tgttttctga
     6541 ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac agacattcaa
     6601 aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc aacaagaaag
     6661 tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat tgaccttagt
     6721 cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt tccgtgatat
     6781 gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt ataaagaaac
     6841 aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc tcctgaaaaa
     6901 attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc tggcggaaga
     6961 acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac aaaaggagcg
     7021 ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc gaatccatcg
     7081 agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact ttctacaaac
     7141 tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc aggaacattt
     7201 gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg ctgtactatt
     7261 ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc gctatatctt
     7321 tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag ccttgttccc
     7381 aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa atgaaacgat
     7441 cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc aattaacgac
     7501 aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg tgaaagaaca
     7561 tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag gaacaacaga
     7621 tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa aagcccggta
     7681 ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt atgctcaact
     7741 gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact ttatgaagcg
     7801 ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc gagttttctt
     7861 gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc tttacaccat
     7921 gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa gtcctttgtt
     7981 aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa gacaactgct
     8041 atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa aagtaattca
     8101 atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat attttaattt
     8161 gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg ggtcaaatac
     8221 cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat tccattcaat
     8281 gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca aaatctctga
     8341 tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt ctatcttctc
     8401 atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta atagtagaaa
     8461 gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag aacgacgaaa
     8521 aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt ccaccataca
     8581 ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat agccttcaac
     8641 gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca cagctaactc
     8701 ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga tttttgcttc
     8761 catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat acttatgctc
     8821 tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag caagacaatc
     8881 tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta aaactggttg
     8941 cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga tcttccagtg
     9001 catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc tcgttttgag
     9061 ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt aatgcttctt
     9121 gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct tcttccatta
     9181 catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca gcgtcttctc
     9241 ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg gaaacaccca
     9301 tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga ttcacattgt
     9361 tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca tagcgcaatt
     9421 cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca ataatatgtt
     9481 ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg tttgcatgcg
     9541 gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt gcataaatcc
     9601 ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat ttacgtccca
     9661 tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc tgttccatta
     9721 tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc ttctcatttt
     9781 tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg aatattgcct
     9841 cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc ctcttttttt
     9901 ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct tccagcatcg
     9961 gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc ttgctttttg
    10021 atattcagca agacataaca agataatcgt tggatccgtg tccatgtata tctttttgtt
    10081 tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt tttcatgcgc
    10141 gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt agaagataac
    10201 aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca agttgaaaga
    10261 tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc taacgcatgg
    10321 cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg atagcctgca
    10381 cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt ttcctttgca
    10441 taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc gggatacaga
    10501 ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga cagttcttga
    10561 aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact gctgtaatca
    10621 aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt ttgcaagatt
    10681 tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac gggaagctcc
    10741 actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt atcgatgata
    10801 gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac ctctgctcct
    10861 gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg attgtactct
    10921 acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata aaaaagaaat
    10981 agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata aactattctc
    11041 gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct atatcgttcg
    11101 gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag gcaaaaccgc
    11161 tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc ttcgtctgta
    11221 aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct taaataactt
    11281 tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg accagaccct
    11341 tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc gcttcctgga
    11401 taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata ttctggaaaa
    11461 acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt ttcttctaac
    11521 gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat atagcacagc
    11581 gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc gagcatattg
    11641 ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc aatcatcaac
    11701 atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa ggcaaccgct
    11761 aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg taaatgacgt
    11821 ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc gtaaatgaaa
    11881 gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac tagcggagca
    11941 tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa aacatgtacg
    12001 atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc aatacgtttt
    12061 acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc gttgatttga
    12121 cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac atttcgaacg
    12181 tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc aatttttaat
    12241 tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt tttgctaccc
    12301 agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca agcaaatggg
    12361 ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat gcaatggctc
    12421 gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct acataaatga
    12481 ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt gcatgaagaa
    12541 tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca agaccatgcc
    12601 agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg attaccattt
    12661 tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca atactagctt
    12721 tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct actccaagca
    12781 catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg ttttctctag
    12841 aataagcagt atattcatta ctatagtcca tattcataca acccttcatt ttgaatataa
    12901 tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat cttttgacgt
    12961 attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg atatggagta
    13021 tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt cagctcatcg
    13081 attttatacc attttggcaa atattctacc atatccccac cgataataaa gtaataatct
    13141 gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact tttcccacca
    13201 cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc taattccaac
    13261 atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg tggctgataa
    13321 gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc agccatcact
    13381 aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg ctttttcttc
    13441 tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt cttagcttgc
    13501 gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct ttgatacttt
    13561 tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac gatttcgcaa
    13621 tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt attttgaagt
    13681 aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac gatcattgct
    13741 gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc tttgctacgt
    13801 aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta aatcaatgct
    13861 ttgcgtttca atacatctac accctttggc gcccagcccg caattactac tggcgctgtt
    13921 acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac agaaaactca
    13981 aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg taataatcca
    14041 cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg aatatttaca
    14101 tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc aaatcttgca
    14161 agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg tttgatttct
    14221 tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat ttgatggcga
    14281 tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat ctcgatttta
    14341 tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc cgctgtattt
    14401 ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac gatatagaca
    14461 tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc atgagatttc
    14521 ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc acgttccttc
    14581 atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac tttgttccca
    14641 actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag tgaaccattg
    14701 aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa ttcattcagt
    14761 aagcgcaaaa agtcgtcatc cgtcaatgag acatcttgga tctcattata atggcgtaat
    14821 ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa agccgtttgc
    14881 ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca gccgatacat
    14941 tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt tttgctaata
    15001 gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa ctatctgttg
    15061 cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca cgtatatcag
    15121 tcataatctg atcgcctacc attactatct catcatctgc taaatccaat tgtttctttg
    15181 ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa agatcaaatt
    15241 tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca acagggattc
    15301 ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct ggattattcc
    15361 atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga ttctttaatt
    15421 gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa tttgaaaaca
    15481 tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca accccgtatt
    15541 atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag aaaaagcatt
    15601 tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa caaaaaacaa
    15661 ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat ctaatcgaac
    15721 aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt tatatcgtgg
    15781 caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta actcgcgtag
    15841 ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac cgtctttcca
    15901 caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt ccagcatcca
    15961 gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac caatgatgat
    16021 ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa tagcctctcc
    16081 ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga tcaaggtaat
    16141 caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc gatagccttc
    16201 aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc ctttttgaat
    16261 accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa tcgcttcgtc
    16321 atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct cttcaaaaaa
    16381 atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat gtgctgtctt
    16441 atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg attttctgtt
    16501 tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag aattcagcct
    16561 tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt cttccagcaa
    16621 aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca aaactggcag
    16681 ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct tcattgctat
    16741 gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt ccttcttgca
    16801 tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt gttgctcgtt
    16861 cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg ataaaattag
    16921 gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca gtgaggactg
    16981 cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat tcgggaaatg
    17041 ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt tcatcaataa
    17101 tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga gggcatactt
    17161 tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac tttgcccaca
    17221 tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga ttgatccgaa
    17281 tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt tctggtgtcc
    17341 aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct atgactgcag
    17401 gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct gtgataaatt
    17461 cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca tggacgatta
    17521 tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa actgcactgt
    17581 ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc cctggagaag
    17641 gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg tgtccttgga
    17701 atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc acctgttttt
    17761 ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg attcgagtat
    17821 tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc gttccggcat
    17881 tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct tctctttttg
    17941 tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg cgttgtaaag
    18001 aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag atctgaactt
    18061 ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt ccaaatgccg
    18121 ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt gattgaactt
    18181 tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga taaccaattc
    18241 tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt cctggaatga
    18301 ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc attgcatcga
    18361 tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg gaggcaaatc
    18421 ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt gtcacgattg
    18481 cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc gggccaatgc
    18541 agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct gaatatatcg
    18601 ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg atttcaccgc
    18661 ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa ggttaattcc
    18721 gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc ggctgaagct
    18781 ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac tttcttacgg
    18841 aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg ttcaagagaa
    18901 agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc tggcataaca
    18961 ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt tttcttagca
    19021 acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat tggataacga
    19081 tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc ctgctttgct
    19141 tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc gttttctaca
    19201 aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag tttcatcgct
    19261 tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt gaaagctggt
    19321 ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac ctcgattcca
    19381 tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc ttgttcagat
    19441 atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat attcatgcct
    19501 cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa atcgaattcc
    19561 gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat atccaaaatt
    19621 attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct aatgaattac
    19681 tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc aagtcaatct
    19741 caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct aatgccagca
    19801 aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc atcgatttcg
    19861 tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt gcttttgctt
    19921 ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg acttgttccg
    19981 gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca gaaccatctg
    20041 gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg atttctccat
    20101 agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga acgcctgctc
    20161 cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca cgattaggat
    20221 cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc tcacaaatcg
    20281 ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg ttacggaatg
    20341 cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta caaatacctt
    20401 ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc attggtacga
    20461 acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg ttttcgattg
    20521 ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga ttcatttttt
    20581 ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca tagatcgaaa
    20641 acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg aaatctttga
    20701 cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc ggtgcgatgc
    20761 cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca ataggtgaag
    20821 tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt tcttatccgt
    20881 gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga ttttgtgcta
    20941 agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc ggaatttgct
    21001 tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt gtgatgaatc
    21061 ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc acagattttg
    21121 ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata tttccaatca
    21181 acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc atttttttca
    21241 atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat tttttgaaat
    21301 cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg tttactaaca
    21361 catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct ttctcataat
    21421 cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt tcttctatct
    21481 tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct tttgcaaaag
    21541 ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca ttttttcctt
    21601 ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg tctgcttatt
    21661 ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc ttaatacttt
    21721 tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat ggatactctc
    21781 atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg tttctttctt
    21841 cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga attgaatctg
    21901 acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat gaaaaggccc
    21961 gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta aaacagcttc
    22021 gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat aatttgcagg
    22081 agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt caatcggcgc
    22141 attcatcact gcaaccattt tcccgcttcc agctggggta gcttctgtca tataagcgcc
    22201 tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag ctactaatgc
    22261 cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat gctccttcaa
    22321 taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt gcgtctgatc
    22381 caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa tctcacttgc
    22441 ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca taccttgata
    22501 ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca atatatccag
    22561 aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc aggaaatgtc
    22621 ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa gtcactaaat
    22681 cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca tcttcaattt
    22741 cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg atgtcagtag
    22801 tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct tgaatttttt
    22861 cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta taatttaaca
    22921 gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc tgtaaatatt
    22981 actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc caataagata
    23041 gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa cttttcttgc
    23101 ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg atgcaaaagc
    23161 agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac gtctcgtacg
    23221 gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc tgaatcttct
    23281 gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact tctggtaccg
    23341 tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa gagaatccca
    23401 cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat tagcttagat
    23461 agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat tttttcagcc
    23521 atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc gatagcatga
    23581 gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta atcgggcgac
    23641 attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg ttttatcaat
    23701 ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca tcttcgttca
    23761 cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt ggaaagatca
    23821 ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat tccaaattgt
    23881 tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt cagtgcatcc
    23941 agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac catttcacga
    24001 tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc caactttaca
    24061 acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac taacttgtta
    24121 atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc tgaagttgtt
    24181 tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga cagatctttg
    24241 aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa gacactaaca
    24301 agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact aattagtttg
    24361 attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg caaccttaaa
    24421 agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa acaactgata
    24481 aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat tttgattatc
    24541 aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt atttcaaaat
    24601 ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag aaaaaatgaa
    24661 aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta aggtcatcta
    24721 tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc agaataaaca
    24781 gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt tcgtcgtagt
    24841 agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt accgtccttt
    24901 ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta ccaataaggg
    24961 atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc tctataaaaa
    25021 aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt tgtctgaaat
    25081 tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt ttgaacagaa
    25141 aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga cttacttgaa
    25201 aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta tgcttcaaaa
    25261 agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc agtttttaag
    25321 aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga aaaagcacta
    25381 cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg tttctcctga
    25441 cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt caaaagaaga
    25501 aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag aacagtttga
    25561 aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc ttctcggctt
    25621 cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga ttaagtcata
    25681 gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag tatctgtttc
    25741 agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct ctacgccttc
    25801 tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag cctccagtcg
    25861 ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc ttatttctgt
    25921 tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa ctcgtttttt
    25981 atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa gcttgggttc
    26041 cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct tgggtagttg
    26101 ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa tctttgataa
    26161 aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga gaataattgc
    26221 ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc tttaaatcaa
    26281 gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta cctgcaatca
    26341 tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc aatgtaaatg
    26401 tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct tctccaccga
    26461 ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc ggtgcatcta
    26521 acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg cttggtgtac
    26581 ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc cccgactgtc
    26641 catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga atattacttt
    26701 gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact aaaggatcag
    26761 cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt agatggcgtg
    26821 cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt tcgacctcct
    26881 aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat tttttcataa
    26941 aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg ttcaatcgag
    27001 tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc ttgataaaat
    27061 tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc ttctaaaact
    27121 tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc aaagtcttcg
    27181 tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa ataagcattt
    27241 ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag cccttgtgtt
    27301 tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc tacatcattg
    27361 ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga gatcaattcc
    27421 tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt aggataagcg
    27481 gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata agaaatcctg
    27541 cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct taatttgctg
    27601 atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc agtaggaatc
    27661 acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg ttcacttgga
    27721 caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac atgggattgc
    27781 ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt atggtaagta
    27841 tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc tgctccgaat
    27901 tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata agcataccac
    27961 atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact tggcattcga
    28021 atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat atagacttca
    28081 tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac tccgctgact
    28141 tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc caaactagtc
    28201 cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta cactttcgta
    28261 tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg tttgataacc
    28321 ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc ggataaattc
    28381 ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat tatacactgg
    28441 aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa cgatcccttc
    28501 tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac cttctatgat
    28561 ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt ttacaatttt
    28621 ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa atgtgtattc
    28681 gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga aaaacaaacc
    28741 gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga acttttgttc
    28801 tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag cttgaatcgg
    28861 cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg tgatcagata
    28921 gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa gctggttcga
    28981 tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga caactttttt
    29041 tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg tattgatgtg
    29101 caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt ccgtcagagc
    29161 acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct tttcactttc
    29221 aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata acatagccta
    29281 caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa aaacgttttc
    29341 ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc ttttgatacg
    29401 ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata tcgcattctt
    29461 tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc attaaactac
    29521 gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata cctactagca
    29581 atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat tttccttcag
    29641 cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta tatggttggt
    29701 acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga atcaaatcgt
    29761 tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag actgctgctg
    29821 ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg ccttcactta
    29881 tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct ttgagtgtag
    29941 ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc aatgcacgca
    30001 tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat gcacgatatc
    30061 ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta tctccaccga
    30121 tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca gctttgtaag
    30181 cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg aattcagtac
    30241 ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct tttggtgttc
    30301 cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct gcatttttta
    30361 atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca aattcagctt
    30421 tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca gccaaaatat
    30481 cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga atttctaaag
    30541 aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca aacgctttta
    30601 caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca acaacgacca
    30661 cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc gctaaaatac
    30721 gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta tcttccattg
    30781 cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca gcatttacgc
    30841 tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa agaaccatca
    30901 aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct ttttcacgaa
    30961 tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct tcttcagccg
    31021 cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc aggtaagctt
    31081 ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc attattcaga
    31141 taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt cgtcagcacc
    31201 ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa cgcccatgat
    31261 tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt taaatttgct
    31321 tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg ctactacgtg
    31381 aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt ttatgtgagg
    31441 gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat ctgtaaagat
    31501 taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa agtattcaaa
    31561 ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg tttatttcct
    31621 ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac cctctttgtt
    31681 ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag agtcaaaggt
    31741 caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa aaaactaggg
    31801 gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca tttttgtgaa
    31861 aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa cgattcactt
    31921 gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct gttatcaaaa
    31981 aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc catttggatt
    32041 tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc aaggaaatta
    32101 tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg gtcagccacc
    32161 tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag aacaagcacg
    32221 acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag tcattgaaat
    32281 tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg ttggtaaaac
    32341 agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc aaaaattaat
    32401 ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg gtatacgagg
    32461 acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag aaaatgtgat
    32521 cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg atggaaatat
    32581 ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa tggttggtgc
    32641 tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac gtcgtatgca
    32701 acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga aaggtttgca
    32761 aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg aagcagccgc
    32821 aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca tcgatttgct
    32881 agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta aaacaatcga
    32941 gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag aagatttcga
    33001 aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag aacgtcagct
    33061 cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg ttgaacaacg
    33121 gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga aaaacttagc
    33181 agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag tagcgaaagc
    33241 gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct ccttcttatt
    33301 tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct acgaactatt
    33361 cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa aacatagtgt
    33421 ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg gtcaattaac
    33481 agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag aaaaagctca
    33541 tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga cagatgcaca
    33601 aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg ccggaactgg
    33661 taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc gttctgtatt
    33721 gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg ggatcatcga
    33781 attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc tagacgatgt
    33841 caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg tcaaagaaaa
    33901 attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac gcacgatcca
    33961 agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta tccatgagtt
    34021 gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag aacgtttggc
    34081 taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga gacggtattt
    34141 ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt cattttataa
    34201 aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg tgagcgccat
    34261 gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc ttgaaaacac
    34321 tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct acactgaagc
    34381 ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc cgaaagaaat
    34441 cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc acgaggatga
    34501 aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac atgaattagg
    34561 cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt gggacaaaa
//
LOCUS       DACEMS010000015        34574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEMS010000015 DACEMS010000000
VERSION     DACEMS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.015"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(178..2070)
                     /locus_tag="IW735_000670"
     CDS             complement(178..2070)
                     /locus_tag="IW735_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043691.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2067..3794)
                     /locus_tag="IW735_000671"
     CDS             complement(2067..3794)
                     /locus_tag="IW735_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043692.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3810..4460)
                     /locus_tag="IW735_000672"
     CDS             complement(3810..4460)
                     /locus_tag="IW735_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1043693.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4664..4960
                     /locus_tag="IW735_000673"
                     /pseudo
     CDS             4664..4960
                     /locus_tag="IW735_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            5132..5752
                     /locus_tag="IW735_000674"
     CDS             5132..5752
                     /locus_tag="IW735_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1043694.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5844..7010)
                     /locus_tag="IW735_000675"
     CDS             complement(5844..7010)
                     /locus_tag="IW735_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1043695.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7022..7375)
                     /locus_tag="IW735_000676"
     CDS             complement(7022..7375)
                     /locus_tag="IW735_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1043696.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7503..8525
                     /locus_tag="IW735_000677"
     CDS             7503..8525
                     /locus_tag="IW735_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1043697.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8964..9398)
                     /locus_tag="IW735_000678"
     CDS             complement(8964..9398)
                     /locus_tag="IW735_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043698.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9508..11367)
                     /locus_tag="IW735_000679"
     CDS             complement(9508..11367)
                     /locus_tag="IW735_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1043699.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11485..12645)
                     /locus_tag="IW735_000680"
     CDS             complement(11485..12645)
                     /locus_tag="IW735_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ1043700.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12952..14376)
                     /gene="araA"
                     /locus_tag="IW735_000681"
     CDS             complement(12952..14376)
                     /gene="araA"
                     /locus_tag="IW735_000681"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1043701.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14392..15090)
                     /locus_tag="IW735_000682"
     CDS             complement(14392..15090)
                     /locus_tag="IW735_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1043702.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15047..16708)
                     /locus_tag="IW735_000683"
     CDS             complement(15047..16708)
                     /locus_tag="IW735_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1043703.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16926..18326
                     /locus_tag="IW735_000684"
     CDS             16926..18326
                     /locus_tag="IW735_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1043704.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18578..19657)
                     /locus_tag="IW735_000685"
     CDS             complement(18578..19657)
                     /locus_tag="IW735_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1043705.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19743..20702)
                     /locus_tag="IW735_000686"
     CDS             complement(19743..20702)
                     /locus_tag="IW735_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1043706.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20714..22237)
                     /locus_tag="IW735_000687"
     CDS             complement(20714..22237)
                     /locus_tag="IW735_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1043707.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22247..22810)
                     /locus_tag="IW735_000688"
     CDS             complement(22247..22810)
                     /locus_tag="IW735_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1043708.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22816..23679)
                     /locus_tag="IW735_000689"
     CDS             complement(22816..23679)
                     /locus_tag="IW735_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1043709.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23693..24589)
                     /locus_tag="IW735_000690"
     CDS             complement(23693..24589)
                     /locus_tag="IW735_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1043710.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24764..26035)
                     /locus_tag="IW735_000691"
     CDS             complement(24764..26035)
                     /locus_tag="IW735_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1043711.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26025..27542)
                     /locus_tag="IW735_000692"
     CDS             complement(26025..27542)
                     /locus_tag="IW735_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1043712.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27740..28630
                     /locus_tag="IW735_000693"
     CDS             27740..28630
                     /locus_tag="IW735_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1043713.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="IW735_000694"
     CDS             complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="IW735_000694"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1043714.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29539..31011)
                     /locus_tag="IW735_000695"
     CDS             complement(29539..31011)
                     /locus_tag="IW735_000695"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1043715.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31648..32010)
                     /locus_tag="IW735_000696"
     CDS             complement(31648..32010)
                     /locus_tag="IW735_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1043716.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32233..32985
                     /locus_tag="IW735_000697"
     CDS             32233..32985
                     /locus_tag="IW735_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1043717.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33125..34048)
                     /locus_tag="IW735_000698"
     CDS             complement(33125..34048)
                     /locus_tag="IW735_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1043718.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34063..34335)
                     /locus_tag="IW735_000699"
     CDS             complement(34063..34335)
                     /locus_tag="IW735_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043719.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10219 a         7196 c         5938 g        11221 t
ORIGIN      
        1 aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct tctcatattt
       61 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat tcggaaaaag
      121 ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg tactattcta
      181 tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc cgtctttttc
      241 catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca caataatgtt
      301 gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc ggttttccaa
      361 taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac tagatgttgc
      421 ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg tgattagctg
      481 acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat atccttctgg
      541 caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga tttgttcgtc
      601 tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa acagccaagt
      661 atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat cacgggtatc
      721 tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa gaaggttgat
      781 cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac caggtttgac
      841 atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt gaacattttc
      901 aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt cattggacat
      961 ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg attggatagt
     1021 gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat attgcaagaa
     1081 cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc ctagtacagc
     1141 gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga ttgccgaaat
     1201 aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa atacttcctg
     1261 gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg tttcttctac
     1321 ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt gtgatttcgg
     1381 cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga ctaacgtcaa
     1441 ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg cactagttac
     1501 aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat tgattgctcg
     1561 agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac gattcatttt
     1621 ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg tcataataaa
     1681 ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg tcgcaatgat
     1741 ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt gttgcatctg
     1801 ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca agacctttgg
     1861 tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga tgatcgcaac
     1921 catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt
     1981 ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc ctgctggtcc
     2041 atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag ttgtgaatga
     2101 acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc tttgccaacc
     2161 agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact tactcgttga
     2221 gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg aagattggca
     2281 tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc tgctgttttg
     2341 actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt tcctcctcct
     2401 tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt agcttgtgcg
     2461 atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata ctgcatattg
     2521 tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat taattctcgc
     2581 aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga accagattcg
     2641 atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc agtcccccct
     2701 atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa tgcaagtttt
     2761 tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt ttttccagca
     2821 aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc caacacttca
     2881 ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc
     2941 ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt accgacttcc
     3001 aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc
     3061 atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt gattgccgta
     3121 tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt cttatcgaat
     3181 gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt
     3241 ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc gatcaaaata
     3301 atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt ttgataagca
     3361 agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa aaacatttgt
     3421 gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga ggccgttcca
     3481 tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc cgtacgcaat
     3541 tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat agcagctaag
     3601 atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata atcgacatct
     3661 ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata taaatctgcc
     3721 agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga gatccgtttt
     3781 acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt cagccaagaa
     3841 agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga tgttgaaaat
     3901 aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg gatcagtaat
     3961 tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc aagcaactct
     4021 tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg actcgtatga
     4081 ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg ataaaaagaa
     4141 gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc gaatcctttg
     4201 aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct gtcacgtctc
     4261 attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg atagaaactg
     4321 cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt taagggtact
     4381 ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt aggcaaatgg
     4441 aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca ttgtcatatg
     4501 acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata gtttttttca
     4561 ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa ttaatgagag
     4621 ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc aatcaattag
     4681 tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag atacaagaaa
     4741 taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg ctgctatctt
     4801 gccaagacaa ttaaaaaatc tagatccaaa gaaactttaa tatattctaa gctatacagg
     4861 caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg ctgtttatat
     4921 cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg ttagattcaa
     4981 aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt tttttagtta
     5041 tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca tagttataac
     5101 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5161 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5221 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5281 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5341 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5401 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5461 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5521 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5581 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5641 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5701 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5761 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg taccttggat
     5821 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     5881 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     5941 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6001 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6061 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6121 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6181 gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6241 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6301 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6361 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6421 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6481 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6541 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6601 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6661 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6721 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6781 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     6841 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     6901 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     6961 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7021 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7081 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7141 ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7201 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7261 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7321 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7381 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7441 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7501 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7561 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7621 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7681 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7741 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7801 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     7861 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     7921 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     7981 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8041 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8101 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8161 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8221 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8281 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8341 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8401 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8461 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8521 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8581 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8641 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8701 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8761 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     8821 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     8881 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     8941 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9001 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9061 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9121 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9181 gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9241 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9301 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9361 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9421 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9481 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9541 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9601 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9661 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9721 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9781 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
     9841 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
     9901 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
     9961 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10021 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10081 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10141 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10201 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10261 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10321 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10381 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10441 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10501 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10561 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10621 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10681 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10741 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10801 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    10861 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    10921 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    10981 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11041 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11101 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11161 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11221 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11281 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11341 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11401 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11461 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11521 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11581 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11641 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11701 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11761 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    11821 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    11881 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    11941 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    12001 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    12061 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    12121 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    12181 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    12241 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    12301 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    12361 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    12421 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    12481 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    12541 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    12601 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    12661 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    12721 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    12781 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    12841 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    12901 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    12961 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    13021 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    13081 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    13141 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    13201 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    13261 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    13321 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    13381 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    13441 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    13501 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    13561 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    13621 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    13681 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    13741 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    13801 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
    13861 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    13921 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    13981 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    14041 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    14101 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    14161 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    14221 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    14281 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    14341 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    14401 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    14461 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    14521 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    14581 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    14641 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    14701 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    14761 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    14821 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    14881 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    14941 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    15001 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    15061 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    15121 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    15181 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    15241 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    15301 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    15361 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    15421 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    15481 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    15541 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    15601 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    15661 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    15721 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    15781 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    15841 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct acactgttcg
    15901 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    15961 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    16021 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    16081 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    16141 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    16201 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    16261 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    16321 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    16381 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    16441 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    16501 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    16561 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    16621 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    16681 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    16741 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    16801 tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    16861 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    16921 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    16981 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    17041 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    17101 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    17161 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    17221 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    17281 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    17341 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    17401 attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    17461 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    17521 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    17581 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    17641 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    17701 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    17761 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    17821 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    17881 gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta ccagctgttt
    17941 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    18001 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata ggcgaaatat
    18061 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    18121 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    18181 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    18241 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    18301 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    18361 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    18421 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    18481 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    18541 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    18601 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    18661 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    18721 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    18781 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    18841 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    18901 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    18961 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    19021 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    19081 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    19141 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    19201 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    19261 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    19321 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    19381 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    19441 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    19501 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    19561 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    19621 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    19681 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    19741 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    19801 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    19861 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    19921 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    19981 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    20041 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    20101 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    20161 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    20221 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    20281 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    20341 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    20401 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    20461 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    20521 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    20581 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    20641 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    20701 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    20761 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    20821 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    20881 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    20941 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    21001 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    21061 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    21121 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    21181 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    21241 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    21301 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    21361 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    21421 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    21481 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    21541 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    21601 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    21661 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    21721 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    21781 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    21841 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    21901 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    21961 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    22021 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    22081 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    22141 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    22201 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    22261 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    22321 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    22381 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    22441 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    22501 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    22561 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    22621 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    22681 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    22741 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat ttagtctcaa
    22801 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    22861 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    22921 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    22981 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    23041 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    23101 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    23161 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    23221 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    23281 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    23341 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    23401 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    23461 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    23521 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    23581 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    23641 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    23701 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    23761 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    23821 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    23881 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    23941 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    24001 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    24061 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    24121 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    24181 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    24241 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    24301 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    24361 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    24421 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    24481 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    24541 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    24601 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    24661 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    24721 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    24781 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    24841 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    24901 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    24961 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    25021 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    25081 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    25141 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    25201 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    25261 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    25321 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    25381 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    25441 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    25501 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    25561 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    25621 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    25681 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    25741 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    25801 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    25861 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    25921 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    25981 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    26041 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    26101 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    26161 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    26221 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    26281 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    26341 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    26401 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    26461 tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca agcaatcttt
    26521 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    26581 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    26641 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    26701 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    26761 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    26821 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    26881 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    26941 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    27001 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    27061 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    27121 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    27181 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    27241 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    27301 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    27361 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    27421 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    27481 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    27541 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata
    27601 atataaatgt taaacttact tattttttat cgattttaat taaccaaaat attaataatg
    27661 attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata taagaaaaac
    27721 cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga
    27781 agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa
    27841 tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa
    27901 aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag
    27961 ccttcgtgtc gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac
    28021 gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg
    28081 tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc
    28141 tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc
    28201 taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc
    28261 tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt
    28321 ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc
    28381 tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa
    28441 acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa
    28501 agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg
    28561 ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta
    28621 ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg
    28681 gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat
    28741 gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa
    28801 cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat
    28861 ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt
    28921 ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg
    28981 gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag
    29041 catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca
    29101 tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc
    29161 ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg
    29221 gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa
    29281 gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg
    29341 ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa
    29401 aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta
    29461 tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca
    29521 ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg
    29581 ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg
    29641 cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc
    29701 tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg
    29761 taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg
    29821 gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc
    29881 ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt
    29941 tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc
    30001 gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt
    30061 tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc
    30121 ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg
    30181 ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa
    30241 gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa
    30301 cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa
    30361 tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg
    30421 tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc
    30481 cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa
    30541 tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg
    30601 gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc
    30661 cacttggatc aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt
    30721 tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc
    30781 acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg
    30841 ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc
    30901 aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg
    30961 atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta
    31021 ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga
    31081 aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac
    31141 tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta
    31201 ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata
    31261 aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata
    31321 taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt
    31381 tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt
    31441 tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc
    31501 acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat
    31561 ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt
    31621 taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat
    31681 cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt
    31741 cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata
    31801 gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc
    31861 atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat
    31921 agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt
    31981 actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta
    32041 gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa
    32101 cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa tttcatgaga
    32161 aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca
    32221 ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg
    32281 agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca
    32341 ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga
    32401 acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac
    32461 cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc
    32521 ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac
    32581 aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga
    32641 caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg
    32701 aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat
    32761 ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct
    32821 cataagaagt ggttatctta cagctggcct gaatatcagc caaaagcgca taaaggacta
    32881 ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat
    32941 ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc
    33001 aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt
    33061 ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat
    33121 atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa
    33181 acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa
    33241 gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat
    33301 agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa
    33361 gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa
    33421 caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt
    33481 tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct
    33541 tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct
    33601 gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat
    33661 catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt
    33721 cttcaagata ccttcgctct ttttctttct gatagcccgt cggtaaaagt gtcactttgt
    33781 aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat
    33841 tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat
    33901 ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct
    33961 catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga
    34021 gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc
    34081 ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg
    34141 cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt
    34201 cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac
    34261 ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc
    34321 tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg
    34381 tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca
    34441 aagtctctta ttttaaattg aatacattat aatgatactg taaatatatc tttattatta
    34501 gtgaatggat atttactttt aatagataaa atattataac tattaaaaat aactattttc
    34561 attataatta caca
//
LOCUS       DACEMS010000016        33787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEMS010000016 DACEMS010000000
VERSION     DACEMS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.016"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            3..74
                     /locus_tag="IW735_000700"
     tRNA            3..74
                     /locus_tag="IW735_000700"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Gln,seq:ttg)
     gene            589..1296
                     /locus_tag="IW735_000701"
     CDS             589..1296
                     /locus_tag="IW735_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1043720.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1356..2000)
                     /locus_tag="IW735_000702"
     CDS             complement(1356..2000)
                     /locus_tag="IW735_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1043721.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2127..2414
                     /locus_tag="IW735_000703"
     CDS             2127..2414
                     /locus_tag="IW735_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043722.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2522..2842
                     /locus_tag="IW735_000704"
     CDS             2522..2842
                     /locus_tag="IW735_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1043723.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2923..4236
                     /locus_tag="IW735_000705"
     CDS             2923..4236
                     /locus_tag="IW735_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1043724.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4233..4952
                     /locus_tag="IW735_000706"
     CDS             4233..4952
                     /locus_tag="IW735_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1043725.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            5080..5631
                     /gene="raiA"
                     /locus_tag="IW735_000707"
     CDS             5080..5631
                     /gene="raiA"
                     /locus_tag="IW735_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1043726.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5853..8387
                     /gene="secA"
                     /locus_tag="IW735_000708"
     CDS             5853..8387
                     /gene="secA"
                     /locus_tag="IW735_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1043727.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8513..9614
                     /gene="prfB"
                     /locus_tag="IW735_000709"
     CDS             join(8513..8575,8577..9614)
                     /gene="prfB"
                     /locus_tag="IW735_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1043728.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9719..10405
                     /gene="ftsE"
                     /locus_tag="IW735_000710"
     CDS             9719..10405
                     /gene="ftsE"
                     /locus_tag="IW735_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1043729.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10389..11282
                     /locus_tag="IW735_000711"
     CDS             10389..11282
                     /locus_tag="IW735_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1043730.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11434..12882
                     /gene="cls"
                     /locus_tag="IW735_000712"
     CDS             11434..12882
                     /gene="cls"
                     /locus_tag="IW735_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1043731.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13219..14076
                     /locus_tag="IW735_000713"
     CDS             13219..14076
                     /locus_tag="IW735_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1043732.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14191..15111
                     /gene="pstC"
                     /locus_tag="IW735_000714"
     CDS             14191..15111
                     /gene="pstC"
                     /locus_tag="IW735_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1043733.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15111..15995
                     /gene="pstA"
                     /locus_tag="IW735_000715"
     CDS             15111..15995
                     /gene="pstA"
                     /locus_tag="IW735_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1043734.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            16010..16816
                     /gene="pstB"
                     /locus_tag="IW735_000716"
     CDS             16010..16816
                     /gene="pstB"
                     /locus_tag="IW735_000716"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043735.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16832..17590
                     /locus_tag="IW735_000717"
     CDS             16832..17590
                     /locus_tag="IW735_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043736.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17603..18280
                     /gene="phoU"
                     /locus_tag="IW735_000718"
     CDS             17603..18280
                     /gene="phoU"
                     /locus_tag="IW735_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1043737.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18518..19549
                     /locus_tag="IW735_000719"
     CDS             18518..19549
                     /locus_tag="IW735_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1043738.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19546..20313
                     /locus_tag="IW735_000720"
     CDS             19546..20313
                     /locus_tag="IW735_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1043739.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20413..21981
                     /gene="liaX"
                     /locus_tag="IW735_000721"
     CDS             20413..21981
                     /gene="liaX"
                     /locus_tag="IW735_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1043740.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22281..22598
                     /locus_tag="IW735_000722"
     CDS             22281..22598
                     /locus_tag="IW735_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1043741.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22598..22948
                     /locus_tag="IW735_000723"
     CDS             22598..22948
                     /locus_tag="IW735_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1043742.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23115..24053
                     /locus_tag="IW735_000724"
     CDS             23115..24053
                     /locus_tag="IW735_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1043743.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            24068..24898
                     /locus_tag="IW735_000725"
     CDS             24068..24898
                     /locus_tag="IW735_000725"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1043744.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24942..25967
                     /locus_tag="IW735_000726"
     CDS             24942..25967
                     /locus_tag="IW735_000726"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1043745.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25982..26920
                     /gene="galU"
                     /locus_tag="IW735_000727"
     CDS             25982..26920
                     /gene="galU"
                     /locus_tag="IW735_000727"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1043746.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            27045..27485
                     /locus_tag="IW735_000728"
     CDS             27045..27485
                     /locus_tag="IW735_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1043747.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27488..28090
                     /locus_tag="IW735_000729"
     CDS             27488..28090
                     /locus_tag="IW735_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1043748.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(28153..29256)
                     /locus_tag="IW735_000730"
     CDS             complement(28153..29256)
                     /locus_tag="IW735_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1043749.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            29466..30467
                     /gene="ccpA"
                     /locus_tag="IW735_000731"
     CDS             29466..30467
                     /gene="ccpA"
                     /locus_tag="IW735_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1043750.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            30822..31217
                     /locus_tag="IW735_000732"
     CDS             30822..31217
                     /locus_tag="IW735_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043751.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            31217..31468
                     /locus_tag="IW735_000733"
     CDS             31217..31468
                     /locus_tag="IW735_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043752.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            31511..31906
                     /locus_tag="IW735_000734"
     CDS             31511..31906
                     /locus_tag="IW735_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043753.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            31903..33159
                     /locus_tag="IW735_000735"
     CDS             31903..33159
                     /locus_tag="IW735_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1043754.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            33171..33494
                     /locus_tag="IW735_000736"
     CDS             33171..33494
                     /locus_tag="IW735_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043755.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            33487..33753
                     /locus_tag="IW735_000737"
     CDS             33487..33753
                     /locus_tag="IW735_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043756.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        11317 a         5812 c         7270 g         9388 t
ORIGIN      
        1 attggggtat agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa
       61 tccagctacc ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tctttttttt
      121 gctttgctac cttgttcctt gaagattctg ctgatttaag atagagttaa gttaactaca
      181 agaattggag ggatccacat gaaatggaaa gaaaaagtag acgaagcagc tgaaaagtta
      241 tatgatttgg tcaaatcaga aaaatacaat attgaagtga atattccgaa aaaaggcgga
      301 aaagcggtcc aggtaaaatc aaaacgccca acgaatcata caaaaaaatg gatggctaaa
      361 aaccgctaat aataacaaaa gatccataag tcggcatttt catcgatttt agggtctttt
      421 gttattttct aggttgaaca tatctaaaaa attgtttctg acaataggag taggaagaac
      481 gatgtgtgtc taaatcctct gttgatggat agaaaccttt atctttttca gttgtgaatc
      541 aggtatatac cagttataat agaagaaacg aaaggacggg tgtacgttat gtcgaatcaa
      601 ttacctgtat atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca aggtatatgg
      661 aaagtcggag accgtcttcc ttcagaaaga gaattagcag tgaaattcaa tgtcagccgt
      721 atgacgttga gacaagcaat tcaaaacttg gcagatgaag gaatcttaga aagaaagatc
      781 ggttcgggta catatgttgc gcgagaaaaa gtacaagaaa aaatgtctgg tgcaacaagt
      841 tttacagaaa ttatggaatc gcaaaatcga gtaccgtcta gtcggacaat ctcttatttc
      901 ttgacttcgc caagctcaag tgaaatggaa aaactaaact tgagtaaaga tgacacgatc
      961 ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta tttgttttga agttgccagt
     1021 gtgccacaaa agctgatcga tggctacagc aaagcagaga ttactgaatc gttgtatcgc
     1081 acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat tactgctgtg
     1141 ttggcttctg agaaaattgc tgactattta gaggtaaaac gaggagatgc agttcttcgt
     1201 ttgcgccaga tttcttattt tgaagacggt acgccgtttg agtatgtgcg tacgcaatac
     1261 gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa caggtgatcc ctgattcctt
     1321 acttagaaat aaaagagaat cagaatcacc tgtttttata tatgatcagt taccagttgc
     1381 tcgtgataag aaacaggacc ctcttcaaat tggacttgtc cgttcagcag ctcaatgatg
     1441 gattctttaa agtggtcagc acttttttca tctacagcac agatgacgct aacttgatca
     1501 gtatattgga tttcttttat aaaaaaatgt tggagttcaa gataattctg taattttcct
     1561 agttgcggat acgtgatcat cacagagatc tcctgttgca aggtaccttc tacgattcca
     1621 atgtcagaca atgcttgaga gacagcatga gtgtatgctc gtattaatcc gcctgtgccg
     1681 agtttcgttc ctccaaaata tcgagtgact actgcagcta cattgatcag ttgatttttt
     1741 ttgaggactt ccaacatcgg tacgccagcc gttccacttg gttctccatc atcgctgctt
     1801 cgctggattt catttttttc tccaatgatg aaggcgctgc agttatggtt cgctttccaa
     1861 tgttcttttt ttatttcttg aatgaaactt tttgcttggt cctctgattc tattcgtcta
     1921 agagaacaga taaagcgtga ttttttgatc tcaatctcac tttgtccatt ttcttttatt
     1981 gtacgatagc gctcgatcat cttgtcacct ctttttttca ttttctttat tcagtataaa
     2041 taaagttcta ggttgtttca acactgtaac ggttgcgcaa tcgctgtagt atgcgataat
     2101 aaagacaaag gtaggtggat ctggggatgc aggaacgaat gaaaaagtac gatgcaatta
     2161 cgcattattt gaagaacaat ggcggctctc aagtaacgtt gacctttacc caatttgacg
     2221 aactgctgtt tcccagtaac ggacttccca aaacagcaag ggaaagtaca gattggtggg
     2281 caaatgatta taaacaccct gaaaaaggtg cctatggctg gataaatgca ggctatgaag
     2341 tagttgtcat caatttggac aaagaatatg tcgtgttcaa taaattagtg aaatctagtt
     2401 ggctatttga ttaaataaac cgcgtttaga aaacgttgac aaatttcttt ctttttatat
     2461 aatagagtaa cagacgcgaa gaaaacgttt gcgtagtttg ttttaaaaag gagagaaaag
     2521 tatggctaaa aaaacaatca tgcttgtatg ttcggctggt atgagcacaa gtttactagt
     2581 aacaaaaatg caaaaagctg ctgaagcaaa aggaatcgac tcggatattt tcgctgtgtc
     2641 cgcttctgac gcagataaca atcttgcaaa taaagatgta gatgtactgt tattaggacc
     2701 tcaagttcgc tttatgaaag ctgattttga aaaacgtctg gaaccaaaag gcattccttt
     2761 agacgtcatc aatatggctg attatggtat gatgaatggc gaaaaagtgt tagatcaagc
     2821 aatcacgctc ataggtgaat aaatagaaca gtataactat cgaacaagcg atccaaaaaa
     2881 tagatcgctt gttttttcgt atattttttt agggcgaata atatgaatca tttttggggt
     2941 agaaggcttc tgacgagaga tattaaagca atagactgtg aggaggtaaa tttaccaaat
     3001 tatgagaaga tcgcagcagt ggaacgtttg ggcaactgga ttctttgtca tcgctgtgga
     3061 atgaggacgt ccgtcattga tggacagctt gcagattacg gttatttttg tacacattgt
     3121 ctatatttag ggcgatgcga cactcaacaa gatttatatt tgtttgatca gcctgaagca
     3181 aaacctcgag aagtcgtctt tttttggaca ggacagctta ctgaaaaaca aacggaaatc
     3241 gcaaagagaa tactttgcca ttcagaaaaa agacatcatc ttatttgggc tgtaacaggt
     3301 gcagggaaaa cagagatgct ttatcctatt ttagtggaaa ctttaaaagc tggaggaaga
     3361 gtagcgattt gtacaccgcg agtcgatgtt tgtaatgaat tgtttttacg ctatcgttat
     3421 gtttttccag aagaaaaaat cacgttgctc catgggaata cagcaacggc ttataccttt
     3481 acttcttttg ttatctgtac gattcatcag ctataccgtt tttatcggtc ctttgattta
     3541 atagtgatcg atgagatcga tgcgtttcct tataacgggg atgcaggatt agctcatgct
     3601 gtgcaaacag cgattaaacc agacggacga ttcatctact taagtgccac acctgataaa
     3661 aagttactaa aggaaatcaa gcagacgttt gacatccaca aacttgcttt gcgctttcat
     3721 cggcgtttac ttcctgaacc cgtcttggtt ttcatgaata attggcgaca aaaatgcaag
     3781 aacaagaaga agataaaatc tttggtgcgg atcattcatc aattaattaa agaaaaccat
     3841 atccttttat tttgtccaag tatcacgatg atgcatcgtt tgaaagccac acttcaagag
     3901 gctcttccga gatatgagat cacagatgtt tcttcaagag acaatcagcg ggcggaaaaa
     3961 gtccagaaaa tgcgagaagc aaaatatgac atactgttaa ccacgatgat ccttgaacgt
     4021 ggagtcacat ttgaaagaat atccgttatc gttttaggag cggatcatcc ggtttttact
     4081 aaatcttctt tggtacaaat cgcaggaaga gccgatcgta aagggcctta tacgaacagt
     4141 caagtatact ttttctatga agaaaaaact gcagctattc gaaaagcttg tcaggaaatc
     4201 aaagaaatga atgaagaagg gaagaagatg ctgtgaggtg cagttgctgc agtaaagata
     4261 tcgtccaaaa tttaacattc agcgagatta ttggtgtttc gaaatacaga ttacctcaga
     4321 cttgttcagt ttgtcgcgca caatttcatt tattggaaac aagaggctgt tctggctgtc
     4381 aaaaaccaat ccatttcaat ctagagagta cgaaaaagaa agtaaagtct attcccgttt
     4441 actgcagtga ctgtcaagaa tggaagaaga aatatccgac ctatgatttt cagcaccgtg
     4501 ccttttttgt gtacgatgaa aatatgaagg cctggctcaa tcaatacaag tttatgggag
     4561 atatccgact agcaggcacc tttgcatata aatggacgca gataaaaaaa gaatacccga
     4621 catacatcta ttgtccaata cctttatctg aaaaaaggcg aacagaaaga ggattcaatc
     4681 aagtaagcga gatgttgaaa gcagcaagaa ttccttttca gatgctactc aaaagagaga
     4741 agcatctgct ggctcaagca aaaaacacgc gaaaggaaag attggagatg cctcaacctt
     4801 ttgaacttgc agtcaccttt tctaagatac aaaataaaaa aatattgatt attgatgatg
     4861 tctatacaac aggacagact cttttccacg cagcagattg tttgcaggtc gcacagccga
     4921 aaatcatccg aaccatttct ttagcaagat gaattagatg tggaaatttg atagcgattt
     4981 aatttgcaaa taacctagag ttcgatataa tagaattaag aagagggaaa gagtggtcct
     5041 tctccttctt gattgtggta gacgaaaggg gtaattttta tgtttagata taatgtacgc
     5101 ggcgaaaata tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa agtaggcaaa
     5161 ctagaacgct atttcagtga cgcacccgat gcaacggcgc atgtgaactt aaaagtttat
     5221 acagaaaaaa cagcaaaagt ggaagtaacg atcccattac cttatcttgt attacgtgcc
     5281 gaagaaactt ctccagattt atacgcaagt atcgatttgg ttgttgacaa attggaacgc
     5341 caaatccgca aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg catgaatact
     5401 gcagatgcta ctgctttatt caatgaagag gacgaaaacg aaaatgattc tgaattagat
     5461 atcgtacgta cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc agtcttacaa
     5521 atgaacatgc tgggacataa cttctttatt tttgaagatg cagaaacaaa tggtacaagt
     5581 atcgtttacc gccgtaaaga tggaaaatac ggactgattg aaacagactg atcagcctta
     5641 cttaaataac aacgaaaact tgcagcagct actgacagct gccgcaagtt tttttgagtt
     5701 tcctcctctt ttagatgaaa tatgaataaa ttatgaagtt cttactttaa tacgcttaag
     5761 atatttcaat tatctataac taatgataaa atgaaaagta aagtctacat agtctggata
     5821 atacacagaa ggaaaaggag attacacaac gcatggccaa tttgattcgt tcacttatcg
     5881 aaaacgataa gaaagaacta aaacgtttag atagcattgc aaaaaaagtt gaagcatacg
     5941 ctgaccaaat ggcagcatta actgatgaac aattacaggc aaaaactgct gaattcaagg
     6001 gcagatacca aaaaggagaa acattagatc aattacttcc ggaagctttt gcagtcgtcc
     6061 gagaagcagc aaaacgtgta ttggggttat atccacacca cgtacaattg atgggtggaa
     6121 tcgtgcttca cgatggaaat atccctgaaa tgcgtactgg tgaaggtaaa accttgaccg
     6181 caacgatgcc agtatatcta aatgctctga ctggcgaagg ggtacacgta gttaccgtca
     6241 atgaatattt agcaacccgt gactcgacgg aaatgggcga actttataat ttcttaggat
     6301 taacagtggg gttgaatatc aactcaaaat cttcggatga aaaacgcgag gcttataatt
     6361 gtgatatcac ttatagtacg aataatgaat taggatttga ctacttgaga gacaatatgg
     6421 tcgtttaccg ccatcaaatg gtacaacgtc cgctgaatta tgccattgtc gatgaagttg
     6481 actcaatctt gattgatgaa gcgagaacgc cgctgatcat ttcagggcaa gcagaaaaat
     6541 ccacagcttt atatacaaga gcagacaact tcgtgaaacg tttgaaagaa gaagaagatt
     6601 ataaaatcga tgtacaatca aaaacaatca gtttaaccga agcagggatc gaaaaagcag
     6661 aagaaacatt cggtttggat aacttatacg acattgaaaa tactgcattg acccatcact
     6721 tggatcaagc attgagagcc aactatatca tgcttttaga tatcgattat gttgtccaag
     6781 acggaaaagt actgattgtt gaccagttca ctggtcggat catggatgga cgccgttatt
     6841 cagatggact tcaccaagca atcgaagcta aagaaggcgt agagatcgaa gacgaaacga
     6901 aaaccatggc gacgatcact ttccagaact atttccgtat gtacaagaaa cttgcgggaa
     6961 tgactggtac agctaaaaca gaagaagaag aattcagaga aatttataac atccaagtat
     7021 accagattcc gacaaatcgt ccggttatcc gtgatgatcg tccagatttg ctttacccaa
     7081 cattacaaag caagttccac gctgtcgtgc aagatatcaa agatcgctac cgcaaaggtc
     7141 aacctgtgtt agtcggaact gttgcggttg aaacatctga gttattatca gatatgctga
     7201 atcaagaacg tattcctcat gaagtgctga atgcgaaaaa tcactttaaa gaagcagaga
     7261 tcatcatgaa tgctggtcaa aaaggtgctg tgacgattgc taccaacatg gctggtcggg
     7321 gaacagatat caagttagga cttggggtaa gagaacttgg tggtctagca gttatcggga
     7381 cagaacgaca tgaatctcgt cgtatcgata accagcttcg aggacgtgca ggacgtcaag
     7441 gagaccctgg tatgtcacag ttctacttgt cattggaaga tgatttgatg aaacgattcg
     7501 gttcagaacg aatcaaatct ttcttagatc ggatgaagat cggtgaggaa gatgctgtta
     7561 tccaaagcaa gatgctgacg aaacaagtag aatctgcaca aaaacgtgta gaaggaaata
     7621 actatgacac acgtaaaaac gtcttacaat acgatgatgt gatgcgtgaa caacgtgaag
     7681 taatctataa acagcgacaa gaagtaatca tggaagaaaa aagcttatcc aagccactaa
     7741 tgaacatggt caaacggaca atcagtcgtg tagtagatgc gcatacacaa ttagagaaag
     7801 aaaattggaa tttggaaagt atcatcgatt ttgccggcag tactcttgtc catgaagata
     7861 gtattactct tgcagatatc gaaggaaaaa cagctcagga gatcaaagag tatctaaacg
     7921 aacgagcaca agaagtattt aataccaaag cggctcaact aaatggtcca gagcaactgt
     7981 tggaatttga aaaagtcgtg atcttgcgtg ttgtggactc taaatggaca gaccatattg
     8041 atgcaatgga tcaattacgt cagtcaatcg gcttacgtgc ttatggacag aacaatccat
     8101 tagtcgaata tcaaacagaa ggctataaga tgtttgaaga tatggtaggc gccatcgagt
     8161 atgaagtcac tcgaatcttt atgaaagctg agatccgtca aaacgtacag cgtgaacagg
     8221 tttctcaagg aacagcttct cactcagaag acggagatgc caaagaaaat agtaatacta
     8281 gcgcgaaaaa acagccaatc catgtgaaca aggtcggaag aaatgatcct tgtccttgtg
     8341 gaagcgggaa aaaatataag aactgtcatg gaaaaaatgc aaactaattt ctgacagcag
     8401 gattggtgag acaaattctt tttttaagaa ttcgtctcac cttctttctg ttatttgctg
     8461 attctgttag actagatata atgaaaaaag tatagtgaaa gaaggaaaaa acatggaaat
     8521 atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag gtcacaagct tcaggaggtc
     8581 tctttgactt agaccagctg gaagaagaca ttgcggaagc agaagcaaaa atggctgaac
     8641 ctggtttttg ggatgatagc gaagctgctc aaaaagtgat cagtgaaaac aatgcaaata
     8701 aagaaactta tgatcaattc cattctcttg ctgaagaact ggaagaaatc gaattgatgg
     8761 tggagatgtt gcaggaagaa cctgatgcag ccatgcaaga agaagcagaa acacgtatca
     8821 atgcgctaga tgaaaaaatg aagacatatg aattatctat gttgttagat ggtccttatg
     8881 atcgaaacaa cgccatcgtt gaattgcatc caggggctgg cggaacggag tcccaagatt
     8941 ggggcagtat gcttttacgt atgtatacac gctgggcaga gcagcatggg tatcaagtag
     9001 agacactaga ttatcaagca ggtgatgaag caggaatcaa gagtgtgacc ctactgatca
     9061 aaggacacaa cgcatttggt tatctgaaat cagaaaaagg tgttcatcgc cttgtacgta
     9121 tctcgccttt tgactcagcg aaacgacgtc atacttcttt ttgttcggta gatgtgatgc
     9181 cagagcttga tgaaacgatc gatatagaaa tcaatccaga tgatttgaaa atcgatacgt
     9241 accgagcaag tggtgcaggt ggtcagcaca tcaataaaac agaatctgct gtacggatca
     9301 ctcacttgcc tacaggaaca gtagtagcta gtcaggcaca acgttcacaa ttgaaaaacc
     9361 gtgagcaagc gatggggatg ttaaaagcta agctttatca attagaagtg gaaaagaaag
     9421 aacaagaagc agctgcactt cgaggagaac aaaaagaaat cggctggggt tcacagatcc
     9481 gttcttatgt tttccatcca tattctatgg taaaagacca tcggacaaac gtagagacag
     9541 gaaatgtcca agcagttatg gacggtgacc ttgatttatt tattgacggc tatttaaaaa
     9601 tgcatctaca ataagaaccc attgttaaat tgaaataaaa ttgtaagatt ttgcaagcat
     9661 gttgaaacat tgccatgcta taatagctaa agactgaaaa agatatggag agaacgccat
     9721 gattgaaatg aaggatgtaa tgaagaagta ctcaaatggt acgaccgcta tccgcaatct
     9781 ctcaattgtc atcgaacaag gagagtttgt ctatgtggtt ggtccgtctg gagccggtaa
     9841 atctacattc atcaaattga tgtatcgaga agaaaaagct tcaaaaggaa ctttgaacgt
     9901 cgcaggtcat gatctaatag caatcaaaaa ccgcgaagtt ccttatctac gcagagaaat
     9961 cggcgtggtg ttccaagact ataaattatt accaagaaaa acagtatatg aaaatgttgc
    10021 gtacgctatg caagttatcg gcagaaaacc tcgggatatc aaaaaacgag taatggaagt
    10081 gctggacctt gttggtttga aacacaaagt tcgagtcttt cctagcgaat tatccggtgg
    10141 ggaacagcaa cgtgtctcta ttgctcgggc aatagtgaat acgcctaaag tacttatcgc
    10201 ggatgaaccg acaggaaatc tggatccaga aaactcatgg gaaatcatga aattattaga
    10261 tcggatcaat gcccaaggaa ctactgtcgt gatggctaca cataacagta cgatcgtaaa
    10321 tacgattcgg caccgagtaa ttgctatcga aaacggccgc attattcgag atcaagcgga
    10381 aggggaatat ggatacgatg attagaacct ttttccgcca tttattagaa agtatcaaga
    10441 gtttgaaacg taacggctgg atgacagttg cttcagtcag tgcggtaacg atcacattga
    10501 ctttagtcgg tgtattcatg gcagttatca tgaacgcaac taaattagct caagatatag
    10561 aaggaaacgt tgatgtctct gtattcgtag atatcggtac aaaagacgaa gagatgaaaa
    10621 agctagaaaa agaattgaat gacttagatc atgttaaaaa agttgaattt tcaagcaaag
    10681 aacaggaatt gaaaaaaatc caaaacgaaa tgggtgattc ctggaaattg tttgaaggtg
    10741 acaataaccc gttatatgat gtgtatgtag taagtgctac tgatccttct tatacaaaac
    10801 aagtagcaga agaagcagca gatttgaaga atgtttctcg ttctgattac ggtggagctt
    10861 cctcagatcg gattttccaa atcgctggtg cagttcggac atggggatta gttgcagcgg
    10921 cattgctgct gttcgtagcg atgtttctca tttcgaacac gattcggatc acgattttgt
    10981 ctagacagcg tgaaattcaa atcatgcgtt tagtcggtgc aaaaaatgga tatattcgct
    11041 ggccgttttt ccttgaaggt ggctggatcg gtctgatcgg tgcgattctt ccgattttga
    11101 tcatcacatt tggttatgca tggtttttcc aattgatcaa tccatcatta ttacgttccc
    11161 attattcatt gattcatcca caaaatatcg tttggaaaat caatctttta atggtcggca
    11221 ttggtgtgat cattggttca cttggctcga tcatttctat gagacgtttc ttgaaattct
    11281 aataccacaa tataaataaa atgagaaagt tggacagttt cgttccagct ttcttttttt
    11341 attggctagg agaatagaag gaagcggctt ttttcccttt actttaaaaa acaacttggg
    11401 tattcttaag ttggtagaag aagaggtgaa gccgtgaaaa ttatcgttga caattttttt
    11461 acgatccttt tgattatgaa tattttgctt tcctttatca tcgtttttcg agaaagaaag
    11521 gaaactgctc aaacatgggc atgggtgctc gttttgatgt tcattccagt tgtaggattt
    11581 attttatata tttttttagg aagagggatt tccaaagaga aaatctttga tttgaagatc
    11641 caagggaaaa tcggtaagaa cctggaaatt gaagaagaca aacaggcatt gatgcgaggg
    11701 ctttatcctc atccgcctac tggacaagta gatgtgaaac aattgatcta tatgctcacg
    11761 gtatttgaaa gtacgctcta tacgactaaa aatgaaatca ctttatttac agatggtcgt
    11821 gaaaaattcg atgcgctgat tgaagatatc aagcaggcag aagaccatat ccacttccag
    11881 tattatattt atcgcagtga tgcattaggt gaagaagttc gagatgcatt gacagaggcg
    11941 gcaagacgag ggggaaaagt cagagtcctt cttgatgcat ggggttccac acaagtttct
    12001 tccagctttt ttcagaattt gaaaaaagca ggcggagaag tcgcattttt ctttcctcta
    12061 tttgttccgt atattaatcc tcggatcaat tatcgcaatc atcgtaaaat cgtagttatt
    12121 gatgggaaaa tcggatatac aggtgggttc aatgttggaa atgaatatct aggattggta
    12181 aagaaatttg gttattggcg agacaatcat ttgcgtattt acggagaagc ggtgtatacc
    12241 ttgcaaaacc gttttttgat ggactggaat tctcagcata caaaagaagt aggctattct
    12301 cccaaatttt ttccgtccat tcactctact ggtgatatag ctgttcaaat cgtgacaagc
    12361 ggaccagata ccgaacatga acaaatcaaa atgacttatt tgaaaatgat ttcgttagcc
    12421 aaacgggaga tattgatcca aacgccttat tacataccgg atggatcgat tcatgaagca
    12481 ctgaagttgg ctcttctttc aggcgtaaaa gttcatatcc aaatacctaa caaaccagat
    12541 catcttctcg tttactgggc aacgtattct ttcgctgcag agctaatcga gtatggagca
    12601 aggatcgaaa catatgagaa tggatttatt catgcaaaga cgatgattat cgatggtggc
    12661 attgtttcag ttggctcagc aaatatcgat gttcgttcat ttcgcttgga ctttgaggtc
    12721 aatacactgg tttatgatga acgaatggct tcacgtgtaa gaaaagcttt ttttgaagac
    12781 tcgaaaattt ctacgcatct cacaaaagaa atgtatgaaa accgaggaat aatcatcaag
    12841 atgaaagaag gattagcaag attgatttcg ccgctccttt aaaacataaa aaggacatga
    12901 gaaccatcaa cagatggaga tctcgtgtcc ttttctaacg gggccatttc tgagagtacg
    12961 tcagaaatgg agaaagcgtc tcgcatgagc gaaattaact ttggggtatc tcttgttttt
    13021 tggggaaagc aagaaataag ctaatatatg ctattaaaag agacacattt caaagccagt
    13081 atagcataga aacttttaat gagatagttt tttttacaat tatgagaatt tattttgtta
    13141 tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag
    13201 gattaaagga gatttctaat gaaaaaactt aaattattct taccaataat aggattagct
    13261 attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc
    13321 tcttctgctt tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct
    13381 ggaaaattca tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca
    13441 ggagcagtcg acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc
    13501 tcagcattag tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat
    13561 attggcgtaa aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa
    13621 aactggagtg aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga
    13681 agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca
    13741 caagaacaag attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc
    13801 agctatgttg ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta
    13861 gaaccgacag atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg
    13921 tacacaagag atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat
    13981 gaagtacaag ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc
    14041 gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt
    14101 agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt
    14161 atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca
    14221 aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg
    14281 ctgatcgtgt tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc
    14341 ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc
    14401 caaatcggag caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt
    14461 attgtgacct ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc
    14521 atgacggaga tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt
    14581 gtaggtattc cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc
    14641 agaagtattt ttggcgggac tgggtttggt atcttagctg gtacatttgt gttgttcgtt
    14701 atgatcttgc cgactgtcac aacgatgact gttgatgcat taaaggcagt gccacggcac
    14761 tatcgggaag cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg
    14821 ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt
    14881 ggtgaagcct tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggtctt
    14941 actactcctg cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg
    15001 ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta
    15061 ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa
    15121 aacgctcaga taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga
    15181 ttttagcagc tttactactt tatatcttag ttcggggaat accgcacatt tcatggtcct
    15241 tcttgaccga accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta
    15301 actcgattta tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg
    15361 gtatctatct ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga
    15421 ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg
    15481 ttattcaatt ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca
    15541 acttgcctct tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac
    15601 gagaagcagg attggcctta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc
    15661 cagaagcgac accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg
    15721 aagcagctgc attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact
    15781 ggaatccatt aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg
    15841 tccatatttg gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg
    15901 gcgcttctgc cgtgttgatt attgctgttt tactgttcaa cttcggcgct cgtgcgattg
    15961 gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata
    16021 taactggaat gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac
    16081 ggatgatcta catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt
    16141 tgaaaaaaat aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct
    16201 tcgttcgttg aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat
    16261 gtatcaaggc gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat
    16321 tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt
    16381 tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag
    16441 cttgaaacaa gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc
    16501 tttatctggc gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga
    16561 tatcttgctg ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga
    16621 agaaacactt gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca
    16681 acaagcagct cgtatcagcg actatactgc ctttttctat ttaggaaaag taatggaata
    16741 tgacaaaact aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc
    16801 cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gagattattt catctaaaga
    16861 tcttcatctg tattatggaa agaaagaagc actaaaaggg atcgatctaa gcttcaatca
    16921 aggggaaatc actgcgatga tcggtccttc tggttgcgga aaatccactt atctgcgttc
    16981 tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa
    17041 agacaaagac atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat
    17101 ggtattccag cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt
    17161 gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa
    17221 ggcagcgtct gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc
    17281 cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat
    17341 cttattggat gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat
    17401 gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc
    17461 ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg aatacaatga
    17521 tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg
    17581 atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct
    17641 ccataatcag ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg
    17701 tgcatacatc aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat
    17761 caataacatg gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc
    17821 agttacaact gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg
    17881 tatggccgat catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag
    17941 aataccggaa atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga
    18001 caacgtccta gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat
    18061 ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca
    18121 ggaaaatcct gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga
    18181 acggatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa
    18241 aatcactgag ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc
    18301 gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag
    18361 taataagaac agtcaaacaa aaaatagctc ctcatatttt tcgtttggct ggacttatta
    18421 ccgcagatgc aagcttttga acaccgttta ttcgggaaag ggtttgtgac aaaacatgtc
    18481 acaaaccctt ttcatcattt catagaaagg ataaagaatg caaaaaaaaa ttagcatcat
    18541 cactatattt tttatttgtg tgcttacttc atgggttatt cagcatcaag ccgctttata
    18601 tgaagtacct gtcatcgaga tagaaaaagt acgaataaaa gaggatcatc aattgattac
    18661 aggaaaactg atgaacacgg atcaaaaagg agaatcgatt tatttatcgg ttccttatca
    18721 aaaaaatcaa atagaaacag caaatataaa agcgagacaa atgtatctag tccaaatgca
    18781 tggagatgaa tggcagctag tagcaaaaaa aaatgatggc tggcttttct tcttcacaag
    18841 tatcttcatt ggcgccatgt tgcttattgg tggaaaacag ggtctatttg ctttaggaag
    18901 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
    18961 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
    19021 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
    19081 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
    19141 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
    19201 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
    19261 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
    19321 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
    19381 agttccatcg tttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
    19441 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
    19501 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
    19561 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
    19621 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
    19681 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
    19741 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
    19801 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcaat ccaaggtttt
    19861 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
    19921 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgcgagtgta
    19981 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
    20041 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
    20101 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
    20161 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
    20221 accacattgt ccgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
    20281 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
    20341 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
    20401 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
    20461 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
    20521 atcaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
    20581 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
    20641 agagaaagac aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
    20701 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
    20761 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
    20821 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
    20881 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
    20941 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
    21001 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
    21061 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
    21121 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
    21181 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
    21241 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
    21301 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
    21361 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
    21421 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
    21481 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
    21541 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
    21601 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
    21661 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
    21721 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
    21781 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
    21841 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
    21901 cgtgtttcag atggtgaaat cgcgcaagtg caactttcaa cgacaacagg aagtatctat
    21961 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
    22021 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgatagtct
    22081 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
    22141 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
    22201 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
    22261 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atctaataaa aatgttgttt
    22321 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
    22381 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aattatttta tacattttat
    22441 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
    22501 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
    22561 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
    22621 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
    22681 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
    22741 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
    22801 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
    22861 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
    22921 tgtcagcgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
    22981 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
    23041 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
    23101 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
    23161 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    23221 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    23281 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    23341 tcatgagaag attatgtagt gaagatatgc cagcatttat tatttcaaga ggattaccag
    23401 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    23461 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    23521 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    23581 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggc catcgattga
    23641 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    23701 ctaaaatcct ggaacatctg attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    23761 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    23821 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    23881 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    23941 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    24001 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    24061 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    24121 ctggtatgca ttgatcatcg tatcaggtat cattatcgct atgtggctta gcagcagaga
    24181 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    24241 tatctccatt attggcgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    24301 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    24361 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    24421 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    24481 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    24541 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    24601 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    24661 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    24721 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    24781 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    24841 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    24901 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    24961 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    25021 acgttcggat ttggggacat catgcttcac aagtagaaga aatcaacacc caacacacga
    25081 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    25141 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    25201 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    25261 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    25321 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcaa
    25381 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    25441 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    25501 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    25561 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    25621 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga ataggcgatc
    25681 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    25741 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    25801 ctacccaagc agcgatggaa ctagcccaaa tgatggaaat cgaaatgccc atcactcaaa
    25861 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    25921 tacgtgatgg aaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    25981 tatgaaagtt aaaaaagcag ttattccagc agcaggatta ggaacaagat ttttaccagc
    26041 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    26101 cgttgaagaa gcgttgaaat caggtatcga agacatcttg atcgttacag gaaaagctaa
    26161 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    26221 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    26281 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    26341 aaatgaacca tttgtcgtta tgctaggcga tgatataatg gaagacgaag taccattgac
    26401 acagcaattg atcgatgact atgagcgtac acacgcttct acgattgccg ttatgcaagt
    26461 tcctcacgat gaaacttcaa aatacggaat catcaaccca ggggaagtac tggaagatgg
    26521 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    26581 agctattatt gggcgttact tattgactcc agaaatcttt gatgtattat cagaacaaca
    26641 accaggtgct ggaaatgaga tccaactaac tgacgcaatc gatacgttga ataaaacaca
    26701 acgcgtattt gcccgtaaat tcacaggtaa acgttatgac gttggtgaca aatttggttt
    26761 tatgaaaaca agtattgaat atggtctgac acatccagaa gtaggggcac ctttgcgtga
    26821 atacatcatg tctttaggta aaacattaga agcagaagga agcaagatca atcataatcc
    26881 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa tctttttttc taacgagctt
    26941 cagcggctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    27001 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    27061 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgttttcatc gttcgcgtat
    27121 tgttgcaagt ttccaaaact ttagaaaaag tagaccagac tgtcgcagaa gcaaatacga
    27181 caattgagat cgtcacacaa gacgtagatc ttttatctag acaagtagaa ggcttgctag
    27241 tcaaaagtaa tgagctgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    27301 tcacagcagt tgctgattta agtgaaagtg tatcggaact taatcattct agtaaaaaca
    27361 tcgtgacgaa agtcggcgga atcggaaaaa cgacagctaa agcgactgtt gccggaaaag
    27421 tcggtggaac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    27481 aataagaatg gctaaaaaag gtggattttt attaggtgca gtagttggag gtacagccgc
    27541 agcaatcgcc gcattattgt tagcacccaa atctggtaaa gaacttcggg atgaattggc
    27601 tgctcaagca gatgatttga tggacatggc ttcagattac acagatatgg caaaagaaaa
    27661 atcatctgag ttaacaggta ttgcaaaaga aaaagcgcaa ggtctttctc aacaagcagg
    27721 ggatctagca ggcaatgtta agcagaaggt aagtgaatca gcagaggaag caggaagtga
    27781 atcagatgat atcttgttaa acctcaaaga acaatcagct gatttatcta atcgtttcaa
    27841 aaaaacagca gaagatctga aaaagccagc agacgaatta ggtagtattg ctaaagatac
    27901 ttcagaagat atcatgatcg atgtaaaaga tgctgcagat aaagcaggag agacggtcaa
    27961 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatgt tgaaaatggc
    28021 aaaagaagat gcaaaagaag ctaaaaaaga aatgaaagat acaatgacag attcgactac
    28081 caatcactaa gattactcat ttctagaata aaaaaggtga gagcggacca gctctcacct
    28141 tttttatttt cattactcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca
    28201 accatcactt gtcacataca cacaatcttc gatacgtacc cctacttttt caggaatata
    28261 gattcctggt tcaagagaga agcacatccc ttcttcaatc actaagtcgt ttccttctac
    28321 aagagaaggg aattcgtgga cggttgttcc gattccgtga cctaaacggt gattgaaata
    28381 ttcgccgtag ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt
    28441 cacacctggg cgaactgcct cttgtgcggc aagctgtgct tcaagagtga tgttatagat
    28501 tttttcttgg aactcagttg gtttttgata agcaactgta cgagtagcat cactgcaata
    28561 gccattccaa ataacgccaa gatcgaataa cactagttca tttggtgaaa ccttcgtgct
    28621 tcctggtgta ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga
    28681 catttgtgaa acaccttgtt tttttaattg gtattcgatt tctgcgataa ttgcttgttc
    28741 agttacgcct tcttttactg ttttgaagcc aatttcaaag gcaacatcgg cccatttacc
    28801 agcttcgatc agtttgtctt tttcttctac cgttttgatc agttgcagac gctggataat
    28861 agaagttaga tcggtgtaag aatctgtacc tgtcaagata tcagagatag catcgaagcg
    28921 atcgacagtc agagcattct tttcaatacc tacattacca ggttgcccat agcgtgcgcg
    28981 aagctctttt ccgatgatct cccaaggatt ttcgctgtct aagtagccac gtacatcgta
    29041 cgcccagctg ctgttttgag catcttcaac ttccagagca ggagtgaaaa ggaaaggatc
    29101 ttgttccaga gggataaaaa gagctagtac acgctcgtga ggatcacttt catatccgga
    29161 aaagtaagcg atgtggccag ggtcgctgat ataagccaat tctacttggt gttgtgccat
    29221 ccattgacgt agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa
    29281 ttttgtattc aataattgta ccactgattg attaaaagac caacgttctt aaaactgaaa
    29341 aaagtaaaaa ttttcatgaa aacagagata aaacggttgc aattgttgag attatttgat
    29401 atcctatttt agaaatgtaa gacgtatttt cagaaaacat ataaaatatg aataggagaa
    29461 cacaaatgga aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga
    29521 tggctactgt ttcacgtgtc gtaaatggca atccaaacgt caaaccagca acaagaaaaa
    29581 aagtcttgga agttatcgat cgcttggatt atcgtccaaa tgcagtcgca cgtggcttgg
    29641 caagtaagaa aacaacaaca gttggggtaa tcattcctga tgtcagcaat atgttctttg
    29701 cctctttagc acgtggtatc gacgatgtgg caacaatgta caaatacaat atcatcttag
    29761 caaactcaga tggcaacgat caaaaagaag tcaatgtatt gaataattta ttggctaagc
    29821 aagtggacgg cgtgatcttt atgggtcacc atatcacaga cgaaatccgt ggagaatttt
    29881 cacgttcaaa aacgcctgtt gtactagctg gttccattga tccagatgaa caagtaggta
    29941 gcgtaaacat tgattatgtg tcagcaacaa aagatgcagt aaataaatta gcaaaaagcg
    30001 gcaataaaaa aatcgctttt gtcagcggtg cgctgattga tcctatcaat ggtcaaaacc
    30061 gtctaaaagg ctataaagaa gcattaagcg aaaacaatct accatatagc gaaggattga
    30121 tttttgaggc acaatacaac tttaaagatg gtctgtctct agttgaccgg atccataata
    30181 gcggtgcaac agctgtctat gtttcagacg atgaattagc cattggtatc ttggatggtt
    30241 tattagaccg cggcgtaaaa gtaccagaag aattcgaaat catcacaagc aacaattctt
    30301 tactgacaga agtagctcgt cctcgtttga caagtatcac acaaccatta tacgatttag
    30361 gagcggtatc catgcgccta ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa
    30421 cgattatctt gccttatgga atcgaagaaa aaggatcaac taaataataa aagatcaatc
    30481 atttaaaggt tgttccaaaa gtggtcaact tctggaacaa cgtttatacg agaaaggggg
    30541 ttatgacaaa attttgttat aacccctttt tttttgtgac tttaaaaaaa gagaaaatgg
    30601 cggaaacaaa ccttacgtcc atcgtatacg acacaatctg tgcgtagatg ctacattttg
    30661 ttcactacta tcaaaattac tttaaaatga aaacgtgata aaaaagaaag tagaaaagta
    30721 gataagtaac tctaaaaaat ccaaaaaaat ccaggctcga ttgcgtactt atcttaaagg
    30781 agaaacaatc catcatatat aacccgaaag gagacttcta tttgcctaag aaaagacgaa
    30841 agaccatagt catcattttg accattctta ctataggaat cggagggaaa ttttacatgg
    30901 acaaaagaga aatgcaaaga cagaaagatt tactcgcagt agaaaaacag tccgtcaaag
    30961 ttctgaaaaa tacctttgcg gatatcaagg aagtgaagat tgaaaaatct gctcgaaatg
    31021 aaatgacggg ttcataccgg atagttattc taatgactaa taaacaagat caatcgattt
    31081 atttttctta tagtttttgg aaagaaagaa atgagatagg aagttacgga atagttgatg
    31141 aaaaaaagca aaaagaagga aatacgctca ataaagttaa agtaacttac tctaatggaa
    31201 atgaggaaag tatttaatga ttactgacaa acaatatagt gaattaagtg atgctgttta
    31261 ctggctagat cctaaacata gagactatgt acctgaaatg caagaaaatt taagttttaa
    31321 aattaacggc actatgtata aaatcctaaa aatcaaaaac tccttcgacg gcatgcaggc
    31381 gatggctgta gcgccaatcg tccatagcaa attggagaaa aatttcaaaa ataaaaaaat
    31441 tcctgcaaac tttcgcgtac ttaaataatg aaggtgtcaa tacttacata aaaagaaagg
    31501 aggcacgttc ttgtcaagga aaataagaaa actcagctta gccattttat taatcgtcat
    31561 attagggatc ggagggaaag tctatatgga caaaagagaa gcacaaaggc agaaagattt
    31621 actcgcagta gaaaaacagt cagtcaaagt tctgaaaaat acctttgcgg atatcaggga
    31681 agtgaaggta gaagaattta aaaaaaaccc tgttaccgga agttatggtg cacttgtgac
    31741 aatgacaaat aatgaaggga aatctgttta ttttgattat agtttttgga aggagagaaa
    31801 tgaacttggt ggttttggca ttgagaacag agcggtacaa aagacaggag tcactataaa
    31861 aagagtaaaa gtaatttttt ctaacggaca ggaggagtta gtatgacaat aaacgataag
    31921 cattacaatg atattagtga gaaggtttat tggctagatc ccaaatatcc ccgctacaat
    31981 gagggataca aaaagaactc tgttaaagaa tttgctggta tggaatttca aatcctacaa
    32041 atcaaagact cattggacgg catgcaggcg atgtctgtag cgccaatcaa gaacggtaaa
    32101 gtggatcttt ctcaagtagt gattgctttt gcaggaacga acttaagtga ttggaaagat
    32161 ttggaaacgg atgcaagaag tatcatgaat ctctacggga atagaccagg agcaagtttt
    32221 tctgaaaaag caacaacaaa caagatttta tccgttagtg gattgaaaac agcggggcaa
    32281 atcaattcag cgattgcatt tgctaatgaa gtaaaaaagg aatatggaga agctgctatt
    32341 tctactaccg gccattcatt aggtggattc ttagcattat atgtagcagc agagaaacaa
    32401 tggaaaaatg tcggcttcaa cgggccggac ccgtatgatc tcttatcacc agaagcaaaa
    32461 aaatgggtgg aagaaaatcc cggtatgctc accaattacc gaaatcacgc cgattttatc
    32521 ggtaattttg gtggtaatgg cacaggtgct gaagttcgga tcagtatgga aacagcattc
    32581 ttgaatctgc ttgcttccca cagtttaacc tcttggaagt ttgatgaaaa tggaaagctg
    32641 attattcctg aaaatgaaaa caatgttaaa gctcgaaaac aacagaaaga aaaccaagcg
    32701 acccagttgt tttcctatca gttgcaagaa ctgaacaaca tacgtaaaaa actacgatta
    32761 agtggcggcc agctgacagt taatgaacaa atttacttag atgacacaca agctaggtta
    32821 gtagtggaaa agttatcggc aaaaatgaaa agcgctatgg aagaagttat ccgcatcaat
    32881 caaagaggaa tttttgagct agagaaaaaa tggcaagaag gcttgcggca agcacgtgtt
    32941 gtagcccctt cgttgactga acaggaaatc atagaagcat tagctagtgt gggcgccaca
    33001 aaagaaaact tagtcgattc tgcgaaagaa gtctatctgc tacgaatcaa taaggcaaga
    33061 cgtatggggg aggcctttga tacattggcg aaggagatcc aagacaaaat caatgaaatc
    33121 gtggcacgag atcgagaatt agcacagcaa ttcaattaaa ggagaagtag atgaaactag
    33181 aagaattgga aagagattat cgaagtgaac gaagacaatt agaagctcaa gaagatgaat
    33241 tgatccactt taagaatcaa ggattacgat tgatggagca agaggagcag cgactgcatc
    33301 atgtattaaa gaacttagct ttagataatg agcctctgtt tcaagcaaga cgaagttatt
    33361 cgaattatca ggagcattat caacaagccc tacaagtaga acgacgaaaa ctctatcagc
    33421 agcaagatga actagaacgt tcctatcgtc aaaaaatcag acaaataact atggagaaat
    33481 catcttatga atgaattttt aatgcaatta ctaaaagaat tagagaaaaa tctacatcga
    33541 tcaaatgtac gttcagttgg tatggggata tctcagcaaa tttgggcaga aaaacgattg
    33601 acagatcaaa actaccaaat gactgcagaa gaagctgctc aagaattttc taaccgacat
    33661 gtacacttga aaggatcagt aaaagggcaa tttgcagaaa aactaactga attattccaa
    33721 agacaagaaa agaaattaaa acagtttcgt tagtaaagtt atatagaaaa attttgtgaa
    33781 aatggtt
//
LOCUS       DACEMS010000017        33712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEMS010000017 DACEMS010000000
VERSION     DACEMS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.017"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..816
                     /locus_tag="IW735_000738"
     CDS             4..816
                     /locus_tag="IW735_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1043757.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IW735_000739"
     CDS             813..1613
                     /locus_tag="IW735_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1043758.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IW735_000740"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IW735_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1043759.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IW735_000741"
     CDS             3110..4819
                     /locus_tag="IW735_000741"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1043760.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IW735_000742"
     CDS             5031..9383
                     /locus_tag="IW735_000742"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1043761.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IW735_000743"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IW735_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1043762.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IW735_000744"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IW735_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1043763.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IW735_000745"
     CDS             11221..11514
                     /locus_tag="IW735_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1043764.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IW735_000746"
     CDS             11511..11822
                     /locus_tag="IW735_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1043765.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IW735_000747"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IW735_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1043766.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IW735_000748"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IW735_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1043767.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IW735_000749"
     CDS             complement(14623..14844)
                     /locus_tag="IW735_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1043768.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="IW735_000750"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="IW735_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="IW735_000751"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="IW735_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1043769.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="IW735_000752"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="IW735_000752"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1043770.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20242..21027
                     /locus_tag="IW735_000753"
     CDS             20242..21027
                     /locus_tag="IW735_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1043771.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21040..21834
                     /locus_tag="IW735_000754"
     CDS             21040..21834
                     /locus_tag="IW735_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1043772.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22014..22466)
                     /locus_tag="IW735_000755"
     CDS             complement(22014..22466)
                     /locus_tag="IW735_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1043773.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22931..23299)
                     /locus_tag="IW735_000756"
     CDS             complement(22931..23299)
                     /locus_tag="IW735_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1043774.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23449..23931
                     /locus_tag="IW735_000757"
     CDS             23449..23931
                     /locus_tag="IW735_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1043775.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23968..24978
                     /locus_tag="IW735_000758"
     CDS             23968..24978
                     /locus_tag="IW735_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1043776.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25093..25806)
                     /locus_tag="IW735_000759"
     CDS             complement(25093..25806)
                     /locus_tag="IW735_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043777.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25996..26586)
                     /locus_tag="IW735_000760"
     CDS             complement(25996..26586)
                     /locus_tag="IW735_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1043778.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27070..27297
                     /locus_tag="IW735_000761"
     CDS             27070..27297
                     /locus_tag="IW735_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1043779.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27313..28479
                     /locus_tag="IW735_000762"
     CDS             27313..28479
                     /locus_tag="IW735_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1043780.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28713..29756
                     /gene="hrcA"
                     /locus_tag="IW735_000763"
     CDS             28713..29756
                     /gene="hrcA"
                     /locus_tag="IW735_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1043781.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29850..30413
                     /gene="grpE"
                     /locus_tag="IW735_000764"
     CDS             29850..30413
                     /gene="grpE"
                     /locus_tag="IW735_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1043782.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30464..32293
                     /gene="dnaK"
                     /locus_tag="IW735_000765"
     CDS             30464..32293
                     /gene="dnaK"
                     /locus_tag="IW735_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1043783.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32444..33610
                     /gene="dnaJ"
                     /locus_tag="IW735_000766"
     CDS             32444..33610
                     /gene="dnaJ"
                     /locus_tag="IW735_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1043784.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT        11459 a         5813 c         7275 g         9165 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20221 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20281 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20341 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20401 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20461 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20521 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20581 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20641 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20701 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20761 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20821 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    20881 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    20941 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21001 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21061 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21121 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21181 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21241 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21301 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21361 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21421 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21481 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21541 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21601 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21661 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21721 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21781 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa ttaacgatta
    21841 tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat
    21901 ggtaaaattt catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa
    21961 aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat
    22021 acaagggaaa tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg
    22081 acataattga agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat
    22141 tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct
    22201 cggaattgat tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt
    22261 gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg
    22321 aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt
    22381 tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat
    22441 tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact
    22501 tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta
    22561 aaaatcaagt cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag
    22621 tgatattata tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat
    22681 tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt
    22741 actaagtaga aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc
    22801 ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata
    22861 ggattgaaca aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc
    22921 gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa
    22981 tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat
    23041 ccgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt
    23101 ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt
    23161 cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta
    23221 tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatt tgattgatga
    23281 tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag
    23341 aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat
    23401 ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg
    23461 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
    23521 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
    23581 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
    23641 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
    23701 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
    23761 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
    23821 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
    23881 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
    23941 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
    24001 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
    24061 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
    24121 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
    24181 atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag
    24241 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
    24301 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact
    24361 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
    24421 ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg
    24481 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
    24541 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg
    24601 atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat
    24661 gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga
    24721 agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc
    24781 ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg
    24841 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
    24901 ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
    24961 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat
    25021 ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac
    25081 atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg
    25141 atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct
    25201 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
    25261 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
    25321 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
    25381 aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa
    25441 agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
    25501 tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga
    25561 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
    25621 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
    25681 tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
    25741 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
    25801 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
    25861 aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga
    25921 aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt
    25981 aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc
    26041 aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg
    26101 aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc
    26161 tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg attctaacca
    26221 aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata
    26281 atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat
    26341 ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc
    26401 aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga
    26461 agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt
    26521 gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt
    26581 tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta acctacagac
    26641 attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat
    26701 acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt
    26761 taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg
    26821 agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca
    26881 tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat
    26941 gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
    27001 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
    27061 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
    27121 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
    27181 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
    27241 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
    27301 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
    27361 catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa
    27421 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa
    27481 agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta
    27541 tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat
    27601 ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta
    27661 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta
    27721 ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta
    27781 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
    27841 ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
    27901 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
    27961 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
    28021 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
    28081 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
    28141 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
    28201 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
    28261 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
    28321 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
    28381 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg
    28441 gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt
    28501 atctgaattt aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat
    28561 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
    28621 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta
    28681 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
    28741 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga
    28801 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
    28861 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
    28921 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
    28981 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
    29041 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
    29101 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
    29161 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
    29221 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
    29281 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa
    29341 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg
    29401 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
    29461 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
    29521 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
    29581 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
    29641 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
    29701 aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg
    29761 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
    29821 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
    29881 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
    29941 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
    30001 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
    30061 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
    30121 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
    30181 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
    30241 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
    30301 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
    30361 acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg
    30421 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
    30481 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
    30541 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
    30601 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
    30661 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
    30721 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
    30781 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
    30841 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
    30901 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
    30961 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
    31021 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
    31081 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
    31141 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
    31201 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
    31261 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
    31321 tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga
    31381 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
    31441 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
    31501 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
    31561 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
    31621 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
    31681 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
    31741 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
    31801 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
    31861 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
    31921 ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct
    31981 acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
    32041 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc
    32101 tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt
    32161 tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc
    32221 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
    32281 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
    32341 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
    32401 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
    32461 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
    32521 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
    32581 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
    32641 tgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
    32701 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
    32761 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
    32821 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
    32881 caagtataat cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac
    32941 acaaccaact acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac
    33001 accacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga
    33061 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
    33121 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
    33181 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga
    33241 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
    33301 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
    33361 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
    33421 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
    33481 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
    33541 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
    33601 gcggaagtag tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca
    33661 atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg taccaacaaa aa
//
LOCUS       DACEMS010000018        31750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEMS010000018 DACEMS010000000
VERSION     DACEMS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.018"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            363..731
                     /locus_tag="IW735_000767"
     CDS             363..731
                     /locus_tag="IW735_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1043785.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IW735_000768"
     CDS             911..1852
                     /locus_tag="IW735_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1043786.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IW735_000769"
     CDS             1921..2652
                     /locus_tag="IW735_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1043787.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IW735_000770"
     CDS             2796..4976
                     /locus_tag="IW735_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1043788.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IW735_000771"
     CDS             4996..5478
                     /locus_tag="IW735_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1043789.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IW735_000772"
     CDS             5494..6255
                     /locus_tag="IW735_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1043790.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IW735_000773"
     CDS             6386..7720
                     /locus_tag="IW735_000773"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1043791.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IW735_000774"
     CDS             8027..8512
                     /locus_tag="IW735_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1043792.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IW735_000775"
     CDS             8505..9188
                     /locus_tag="IW735_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1043793.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IW735_000776"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IW735_000776"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1043794.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IW735_000777"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IW735_000777"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1043795.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IW735_000778"
     CDS             12804..13433
                     /locus_tag="IW735_000778"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1043796.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IW735_000779"
     CDS             13485..13817
                     /locus_tag="IW735_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1043797.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IW735_000780"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IW735_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1043798.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IW735_000781"
     CDS             14643..16016
                     /locus_tag="IW735_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1043799.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IW735_000782"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IW735_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1043800.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IW735_000783"
     CDS             17071..17511
                     /locus_tag="IW735_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1043801.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="IW735_000784"
     CDS             17655..18356
                     /locus_tag="IW735_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1043802.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="IW735_000785"
     CDS             18353..19474
                     /locus_tag="IW735_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1043803.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="IW735_000786"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="IW735_000786"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1043804.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="IW735_000787"
     CDS             20853..21761
                     /locus_tag="IW735_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1043805.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="IW735_000788"
     CDS             21796..22068
                     /locus_tag="IW735_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1043806.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="IW735_000789"
     CDS             22079..23125
                     /locus_tag="IW735_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1043807.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="IW735_000790"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="IW735_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1043808.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26807)
                     /locus_tag="IW735_000791"
     CDS             complement(26136..26807)
                     /locus_tag="IW735_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043809.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26800..27294)
                     /locus_tag="IW735_000792"
     CDS             complement(26800..27294)
                     /locus_tag="IW735_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1043810.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="IW735_000793"
     CDS             complement(27314..28534)
                     /locus_tag="IW735_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1043811.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="IW735_000794"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="IW735_000794"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1043812.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30610..31737)
                     /locus_tag="IW735_000795"
     CDS             complement(30610..31737)
                     /locus_tag="IW735_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1043813.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10568 a         5670 c         6794 g         8718 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtattact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcttt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaaccatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaacctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg
    30601 tggcaaaaat caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat
    30661 cttatctgtt acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg
    30721 ttcagagatt tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct
    30781 gagagaatgg aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt
    30841 cactacactt ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat
    30901 gtagagcttt ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat
    30961 tgctagcaag gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc
    31021 atatacccat gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga
    31081 aaaatcgaca tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa
    31141 tccaatattc aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc
    31201 aaagtcctca agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt
    31261 tggtattttt acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat
    31321 attcagcgca ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt
    31381 tttaaattca gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt
    31441 ctttcctaaa taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt
    31501 gtattttaga tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg
    31561 agtatccgta cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa
    31621 agcagcttcg gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc
    31681 atcatcattg atatcttcgc cgtttgtata attaagcgag acaaataaaa aagccattta
    31741 tgttacactc
//
LOCUS       DACEMS010000019        31660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEMS010000019 DACEMS010000000
VERSION     DACEMS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.019"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..343
                     /locus_tag="IW735_000796"
     CDS             <1..343
                     /locus_tag="IW735_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043814.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            424..1044
                     /locus_tag="IW735_000797"
     CDS             424..1044
                     /locus_tag="IW735_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043815.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1374..2600)
                     /locus_tag="IW735_000798"
     CDS             complement(1374..2600)
                     /locus_tag="IW735_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1043816.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2672..2830)
                     /locus_tag="IW735_000799"
     CDS             complement(2672..2830)
                     /locus_tag="IW735_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1043817.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3518..4258
                     /locus_tag="IW735_000800"
     CDS             3518..4258
                     /locus_tag="IW735_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043818.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(4350..5537)
                     /locus_tag="IW735_000801"
     CDS             complement(4350..5537)
                     /locus_tag="IW735_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1043819.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            5859..6701
                     /locus_tag="IW735_000802"
     CDS             5859..6701
                     /locus_tag="IW735_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1043820.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            6698..7648
                     /locus_tag="IW735_000803"
     CDS             6698..7648
                     /locus_tag="IW735_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1043821.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            7663..7896
                     /locus_tag="IW735_000804"
     CDS             7663..7896
                     /locus_tag="IW735_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1043822.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            8227..8811
                     /locus_tag="IW735_000805"
     CDS             8227..8811
                     /locus_tag="IW735_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1043823.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            8942..9610
                     /locus_tag="IW735_000806"
     CDS             8942..9610
                     /locus_tag="IW735_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1043824.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            9612..11036
                     /locus_tag="IW735_000807"
     CDS             9612..11036
                     /locus_tag="IW735_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1043825.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            11054..11623
                     /locus_tag="IW735_000808"
     CDS             11054..11623
                     /locus_tag="IW735_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1043826.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            11733..13487
                     /locus_tag="IW735_000809"
     CDS             11733..13487
                     /locus_tag="IW735_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043827.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            13441..15213
                     /locus_tag="IW735_000810"
     CDS             13441..15213
                     /locus_tag="IW735_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043828.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            15736..16005
                     /gene="rpsN"
                     /locus_tag="IW735_000811"
     CDS             15736..16005
                     /gene="rpsN"
                     /locus_tag="IW735_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1043829.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            16020..16199
                     /gene="rpmF"
                     /locus_tag="IW735_000812"
     CDS             16020..16199
                     /gene="rpmF"
                     /locus_tag="IW735_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1043830.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            16231..16608
                     /locus_tag="IW735_000813"
     CDS             16231..16608
                     /locus_tag="IW735_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043831.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            16629..16778
                     /gene="rpmG"
                     /locus_tag="IW735_000814"
     CDS             16629..16778
                     /gene="rpmG"
                     /locus_tag="IW735_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1043832.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            16802..17785
                     /locus_tag="IW735_000815"
     CDS             16802..17785
                     /locus_tag="IW735_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1043833.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            17815..17937
                     /locus_tag="IW735_000816"
     CDS             17815..17937
                     /locus_tag="IW735_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1043834.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            17955..19478
                     /locus_tag="IW735_000817"
     CDS             17955..19478
                     /locus_tag="IW735_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1043835.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTDEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            19502..19741
                     /locus_tag="IW735_000818"
     CDS             19502..19741
                     /locus_tag="IW735_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043836.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(19934..20395)
                     /locus_tag="IW735_000819"
     CDS             complement(19934..20395)
                     /locus_tag="IW735_000819"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1043837.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(20590..20808)
                     /locus_tag="IW735_000820"
     CDS             complement(20590..20808)
                     /locus_tag="IW735_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1043838.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            20926..22542
                     /locus_tag="IW735_000821"
     CDS             20926..22542
                     /locus_tag="IW735_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1043839.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(22783..24123)
                     /locus_tag="IW735_000822"
                     /pseudo
     CDS             complement(22783..24123)
                     /locus_tag="IW735_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(24399..24554)
                     /locus_tag="IW735_000823"
     CDS             complement(24399..24554)
                     /locus_tag="IW735_000823"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1043840.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(24746..24940)
                     /locus_tag="IW735_000824"
     CDS             complement(24746..24940)
                     /locus_tag="IW735_000824"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1043841.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(24850..25182)
                     /locus_tag="IW735_000825"
     CDS             complement(24850..25182)
                     /locus_tag="IW735_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1043842.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(25696..25887)
                     /locus_tag="IW735_000826"
     CDS             complement(25696..25887)
                     /locus_tag="IW735_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1043843.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(25981..26367)
                     /locus_tag="IW735_000827"
     CDS             complement(25981..26367)
                     /locus_tag="IW735_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1043844.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            27061..28266
                     /locus_tag="IW735_000828"
     CDS             27061..28266
                     /locus_tag="IW735_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1043845.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            28283..28717
                     /locus_tag="IW735_000829"
     CDS             28283..28717
                     /locus_tag="IW735_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043846.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            29334..>31660
                     /gene="esp"
                     /locus_tag="IW735_000830"
     CDS             29334..>31660
                     /gene="esp"
                     /locus_tag="IW735_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1043847.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT        10868 a         4614 c         5822 g        10356 t
ORIGIN      
        1 aaatataaaa actagtacga aattaatttg taaattattt aagcttgata aaataagcaa
       61 tatcgatgat gtaaagtcca agatattaac aagaaaagta agcgaaccag taaagctaac
      121 ggcacaagat aaaaaaaatg gaattgttct ttatggtgat gatgcaaagt ataactataa
      181 ttgggatacc tcctcagaag ctgaggtatt aagtattcct actaccgata cttcagggga
      241 aaaagtcacc tttaaaataa attttataac ggcaatttct aagctggagt atgggaagca
      301 aaaaaagaga gataacaatg atcaatatct acctaaaaaa taacgaatta atgatgcata
      361 tgttaaagtt gtttttttga attaaacaac tcaatttatt cagttatatg ttcatcgcag
      421 caagtgaaca ttaatcgagt tataaaatta ataggagcta aaaatataaa taaagttcga
      481 tcagaattta tttttccaga agattttttt aactggtttt tttatcaatt cagtgaatgc
      541 aagcgactct taggtgagaa ttattactta aaaggaatcg gcggatttat ggggaatgtg
      601 gctgatgaac aaaatattat aaaaggaata tctgaacaaa catcagagct tacagtaact
      661 aaagcattta taagtaatgg tgaaagtttg aaaaatgtaa ctgcgcgaat tagaaaagaa
      721 aatgaagtag aaattgtttt tggaattgac catgaatgta attcagtgat atatgtaaca
      781 caatcagcaa atttaattct ttttgagaat ggttgcaagg acgattttat ggtggtctat
      841 ttgtacgata tactaattcc aaaacttcgt tccttatata tgtcttctga aagtattttt
      901 gtgagtgaac aaaagggatc tttctcgaaa aaaataggta ttgaagtaat tgaatcgatt
      961 atgtgtcata atcatattac gctagaagaa ttagaaattt tgatagggca aaaaaataat
     1021 aacacgaaaa tacaaatttc ttagaaatca ttttttattg agatatctag ataaatgatt
     1081 tattcttcaa tcaaccaact cgccaagctg aggtaatgtt ttgaggtaat gataacatac
     1141 aactttttat accctagtaa aactctataa acaaagactt attaatttcg catgaaatac
     1201 attcctgtaa aatgcaataa aatagagttt tgtttaacca ggtctcaaaa aggctcgtaa
     1261 agcatctcaa ttctctaaac gttaatatta tttatatatc aatgtttcaa ggcacttttc
     1321 aattgtttga aaagtgtctt tttcttgttt aagaacacat ttaagaacac atactaatca
     1381 catttattca atcctctctg aaaggcttca aaaacagttt tatctactac atctggtgta
     1441 tttacgtata ttctcgttgt agaaatatct gagtgtgtta aagcactaga tatttgattc
     1501 atatttgctc cgccttgtct agctatagtt gcataggtat gacgaagctt atgtggagat
     1561 aaatgagcca attcaggatg tcgtctttta attgaattga ttcgatagtt taggtaatca
     1621 gcgtgaacag ggcaatttac ttctccggat ggtttagtat aggtaaagag atactgatct
     1681 ggtgtttgat taatttttaa tctcaacaat tgctcagctt gcactgattt ccatttgctt
     1741 agtaatgcca ttacaatttc gggtacttga attacggtat cctttcgacc ttttgtaaat
     1801 ttcttttttt gatatttatc caaagcatgt ttaaggcgaa ccgtttgttt ctcaaagtcg
     1861 atatatttcc attgcaaagc atacgtctca ctttttctat caccaagata tagggtaagc
     1921 atgaaaagag tatagtcatg aagagtaagc aagtgattat tcaagtcagt gtttactgct
     1981 tctatccaag caataagctc ttctgacgat aatgcttgat taccttcccg tatccttctt
     2041 tcttctaaag caattttttt aggtgcagtg atacattgaa taattttggt agttcggtta
     2101 aattcaatat aatttagtat ttctgcaata tcaaacatag aacggacata tcctttgata
     2161 atttttatat ttgcgtattc tttcgatttt tttgttaatt catctgatat tatctcagta
     2221 tttgaattta gatagttcat tgcatactca ccaaacattg gaaggatgtg caatcggaat
     2281 atatctttag tattactaat tgttacgtaa cttggagctc tttttgatct agtagtctga
     2341 cctagttcat atctaggaag ccattttgat tcatagaatt ccttaaattt tatttttcct
     2401 tttaattcta gtgaatttgc attctcctct cttaaaactg tttcaatttt ccttttcatt
     2461 gcattttcgg ctttaatagc caattgccta gttggaaata ctttttgata cttttctgat
     2521 gtaattccag tgatttctct gaattttttt ggatacctca gattagtttt atatttccca
     2581 tttgaaagtt ttgtaggcaa aattcctcac tcccttaaaa agtaaggcag tatatttcgc
     2641 tattcttagt atatgcggcg agaagaaatt ttcaaattgt tttaaatgta ttcataaaat
     2701 ctttgatatc ctctatgtca aagcgaacta taccgtcaat tctgattact ggaaaattat
     2761 atatacgaat ccacttatct aaagtattat tatcaattcc tagaaactca caggtttgtt
     2821 ttttattcaa gtagactctc aattttataa aatcatctcc taaaaaagat tagaattttt
     2881 gaattgcttt gggtaagcac ataggaattt cacctctccc cagtttacgg cgagccttac
     2941 ggaagtatca ttatttgctg ctattgtcat ggcattttgt tttcccagaa caaaaagaaa
     3001 tctctaagta tttgtcgcta atcaggtcat ggcgtacttg agtttctagc tcgaatagca
     3061 gggatcgtta ttcaaaaatt gtatttaatt gtcaatgatc aaaaataggt tttttcctat
     3121 tcactattaa taacgataaa catgtataaa aagacacact acgaaaaaag ttaaatggag
     3181 tttactgatt ttcatatttg tatatggaac agacatagtc ttgaataatt tttataaatg
     3241 ttttgatgct tatcaactta ttcaacccgc tatccacttt aagtataatt tttccatacg
     3301 ccaataaagt agttttagga gcatgttctc atttgagaac gctccttttt tatatatttt
     3361 gaaagctact tactatacgc gtagtttcaa atagttttgt cattctaatc ttcagcaaca
     3421 gaaaaaaatc taaattataa aatttagggt tgactaaaat ataatttttg tataccatat
     3481 gatcgtgata atcattatca tttagaagga gaaagttatg ttagaagtga aaaagcttaa
     3541 tgtttgttat aacgattttt tagcattgaa cgagatttct ttaaaaatac aagaaggtgc
     3601 tttaacaggt attattggtc caaatggtgc tggaaaatct acattgctta aagctatgtt
     3661 aaatattatc cctcatcagg gagaggtctc cattaataat gaagatatca ataaaaaact
     3721 ggtgaagatt gcatatgtag agcaaaaagc tgatattgat tttacttttc caataaaagt
     3781 aaaagaatgt gtatctatgg gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa
     3841 tgcagaatgg caaagagttt caaaggcttt ggaaaaggtc gatatgggaa aatattctaa
     3901 tcatcaaata ggagaattat ctggaggaca atttcaacgt gtacttttag caagatgtct
     3961 tgctcaaaat gcggatttca tatttttaga tgaaccattt gtagggatag acttagttag
     4021 tgaacgaata attatggata cgttaaaaga attgaaacat cagggaaaaa cagtgctaat
     4081 tgtgcatcat gatttaagca aggtaaagaa gtattttgat aatattattc ttttgaatcg
     4141 aaatctaatt gctcacggaa gtgtagaatc tgtatttaat gaagagaatt taaaaaaagc
     4201 ctatggagat actatattta ttggagagac taactatata gagtcaagta aaaattagcc
     4261 aaatcaaaaa atattttata acagataatt ccgaaaaaag ggtacaacaa ataagcttct
     4321 taccattagt ttttttgaat gttcaatagc taatcagaaa caattatata aggctcgttt
     4381 ttagtcaaaa tagcatgaat cacgtaacaa agcttacgag aaaccgcacc taaggcagtt
     4441 agatgatgtt ttccttcgga aacttttttc tggtaaaacg cactaaaaac aggatcacat
     4501 tgagaagcta ctaaagccgc cgaaaacaag gcttttcgca aatatggaga ccctcgttta
     4561 ctcataacat tatgcgttgc ctcatattgc cctgattgtg tcacggaagc gtctatccca
     4621 gcgtaagcaa cgagtttttt cgggttagaa aatttttgaa tatctccgat ctcaccaaga
     4681 atagtcgcac cgtttatagg accaattcca ggaattgtga gaataacaga atttaaagtg
     4741 tccattatgt gcttgatttc tctttccgtt tcctttattt ggctttcaat gaattttaat
     4801 tgttcaatca ttgaacgtag ttggaaggtg aacgcttctt gagcaaattt tactccaaag
     4861 gagttagaag ctgcttgctt caattggtcc gctttttctg ccttgattcg gttcctgctt
     4921 aactcagcta agtattgggc caaagtttct gaggaaatgt tttctaagtc gatcggggca
     4981 ctaaacgcca gcaataactc tttagaagct tttccgaaaa cgccctgctt ggtaaaaatc
     5041 gtcgcgtatt ctggaaagac ttgatccaat acggctataa tttttctttt gaagtcgctc
     5101 gctgtaccta ctaaataagt tcggtaacga gaaagttgtt tcaaagaaaa aacattttca
     5161 tcggataaga ccgtttcgac aaatgagcca tagcgcatta agtcagcaat caaaacagaa
     5221 tcaataatat cgttttttcg tttccgtatt tctgtacctt tacgccatcc gtctgtttga
     5281 aggggattca caacgtgaat caaataatct gattcgtgaa ggtatgaaaa gatggacagc
     5341 caatagtgcc ccgtggcttc catccctata acaaagtcac tgggtgctcc ggaatagtcg
     5401 accattcgct caattaatga ctctgctcct tcagtggtgt taggaaatga aaaacctttt
     5461 agtaatacct ttccttcctc gttcatgata gaagcaacgt gagtgcgttt accgatatca
     5521 atgcctaaaa agtacatgta tatctgacct ttcaaaaaaa gaattttgga ttgaaatcca
     5581 ctcaactgaa aagcgtcact accttgtacg agatacggag taaagtaagg actttcaaca
     5641 tctaactcat acgcaatcca cttatcagag agaggagcca gtctctcgag tgcgggttca
     5701 atggcccaag gggggaacgg cttagcctct caatccaata aatatattat ctcaaaagaa
     5761 atgagttaat accaaattaa aataacgatg gttcataggg ttatccaatc aaaaccggaa
     5821 ggttaaaacc ctaactctaa tatacaaggg ggaataatat gcttcaaaat tttattaatg
     5881 gtctgtatga ctttcacttt ttacagaatg ctctaattac atctgtagtt atagggattg
     5941 tttctggaac agtaggttgt tttattattt taagaggaat gtctcttatg ggtgatgcta
     6001 tttctcatgc tgttttgcca ggagtagcaa tttcttatat tttaggtatt aattttttta
     6061 ttggtgcgat tgtatttggt ctattagctt ctatcattat tacttttata aaaagcaata
     6121 gtgtaataaa aggagataca gcaattggta ttactttcag ttctttttta gcattaggag
     6181 ttatccttat aggagttgca aatagctcaa cggatttatt tcatatctta tttggaaata
     6241 ttttagctgt acaagatatt gataagtgga ttacaattgg cgtttctgtt ttagtgttgg
     6301 ttacagttgt tctatttttt aaagaattat taatcacttc ttttgatcca ttaatggcaa
     6361 aagcaatggg gatgaatgtc agtttttatc attatctatt aatgattcta ttaactttag
     6421 tagcagtaac agctatgcaa agtgtgggaa ctatattaat tgttgcaatg ttaattacac
     6481 cagctgctac agcatattta tatgcaaaca gcttaaaaac tatgatattt atttcggcga
     6541 cagtcggagc gtgctcatca ttgctaggtt tgttcattgg ttatagtttt aacatagcag
     6601 caggatcaag tattgtttta acagcggcat tcttatttgt aattagtttt ctaatctcac
     6661 caaaacaaaa atttttaaga aagaaggaga gaaagttatg atgagaaaat ggaaagcagt
     6721 actgggaagt ctggggattt tgattgctct ttttatattc ggggcatgtt caacaaatag
     6781 taaagacaaa gatacagtgg cttcgaacga aaaattaaag gtagtagtta ctaattcgat
     6841 tttagcagat attactgaaa atatagcaaa agataaaatt gatttacaca gtatcgtacc
     6901 tattgggaaa gatccccacg aatatgaacc tttgcctgaa gatgttcaaa aaacttcaaa
     6961 agcagatttg attttttata acggtgttaa cttggaaact ggaggaaatg cttggtttac
     7021 aaaattagta aaaaatgcga acaaagagga aaacaaagac tattttgcag caagtgatgg
     7081 catagatgtt atttacttag agggtcagag tgagaaaggg aaggaagatc cccatgcttg
     7141 gttaaattta gaaaacggta ttatttacgc taaaaatatt gaaaaacagt tagcggaaaa
     7201 agatcctgat aataaaaaat tctataaaga aaatctagat aagtatattg aaaaattgga
     7261 ttctctagac aaagaagcta aatctaaatt tgcttcaatt ccgaatgata aaaaaatgat
     7321 tgttacaagt gaaggatgct ttaaatattt ctcgaaagcg tataatgtgc cttctgctta
     7381 catttgggaa atcaacactg aagaagaagg aacaccagat caaataaaac acttagttga
     7441 aaaattacgc acaacaaaag ttccctcctt attcgtagaa agcagtgtgg acgatagacc
     7501 aatgaaaaca gtatcaaaag ataccaatat tcctatctat tcaacgattt ttactgattc
     7561 aattgcagaa aaaggacaag atggtgatag ttactatgcg atgatgaaat ggaacctgga
     7621 taaaattgct gaaggccttt cgaaataatt tggagggtag gtatggtaca aattaaaacg
     7681 actactatac taatttattt actagcaatt caggaattat caaaaagaaa aaagaaatta
     7741 aaaaataatg atttagcaaa agttctaagt gttagtccag catctgtaag tgagatgctg
     7801 gctaaactta gatatgagga ctatttagat ataggatttc aattaactga taaagggaaa
     7861 aaatttattg acgattacaa aaaaggtttt aagtaaaaga ctttattttt agggaggata
     7921 agaaaatggc tattggagtg attatgatag gacttaccct aatagctgga tataattttt
     7981 ttaaatcttt ctcggattat tttagaaaag gtgaatgatt tagatgagtc tattaattct
     8041 tgtaggattg ctttttgtat cagttatttt gctctttctc attagtttag ctctaaggaa
     8101 tgagttcagt aatagacgtt aacgattgaa acttcaagtc ctgtgtaaga taatatagtt
     8161 aattcatctt attaggagga agtacccata gaaaccagag attatattac aactcaccaa
     8221 aaattaatag aaagtggact aagccatttt gaaaaagaag gatttcagca aactaagata
     8281 aggcaactgt gcttgaccgc aggcgtaact acgggtgctt tttataaaca ttttacttct
     8341 aaggaagcgt tatttgaaga aattattcag ccgcatatta atgcgcttat taatatgtat
     8401 aatgaagagt ttaccaaata cctcttaaac ttagaaaata tagatataga gaaaagttgg
     8461 agagagaact tcaatgaatt agatccattt attaattata tttttgagaa taaacgtata
     8521 ttcgatttgg ttttattcca atcagctgga accaaatatc aagatattgt tgaaaaaatt
     8581 acatcatttg tgacaaaaaa gacagtctgt tgtctagata agttaaagac tatcggaatg
     8641 atcaatcaaa atttagtctt taatgaagaa gaaatccact tctacattta ctcattctac
     8701 gctacattct atgatatatt gaagcatagc tattcaaaag aaaagactat gctattaacc
     8761 agaagattgt atcaattttc tgttcctggg tggattgaat tactgagtta attaaaaaaa
     8821 agcttgcatt gagaattgtt ctcagttatg ctttttgata agatcgcact gttatctgaa
     8881 taacggtgca tttttgtcag cttaagataa cagtgcgatc ttattttttg gaggtgaaag
     8941 agtgtcaaaa atcaatccgt taaccttgat tactttaaat attttttttc cggtcattat
     9001 ttttttagga aagggaaata tatttcaagt ggggtgtttc attattgcta cagttttagt
     9061 attgatgttt cgtaaaataa agacatgtat ttatttcatc ttgatttatt cagttctgtt
     9121 tttagtaatg aaaattattg atgctatcaa gatagagttt tttactatat tttttgggac
     9181 atccttatat attgtatttc gaatgttgcc ggtcgtaatg atctcatggt tgttggttag
     9241 tgtctatact agcaatgaat tattatcctc tttagaaaaa atacatttac cgaaaaaaat
     9301 aatgttaagt attacagtag tgatcagatt ctttccgact tatcgttctg aaataagaat
     9361 gattaaagaa tctttaaaaa tgagaaacat tcgcttgtct ttttttcagc ctctgcaata
     9421 tttggaattt ctgttagttc ccgttcttat acgtgcaact atgattgctg aagaaatgac
     9481 cgctaatgca ataacaaagg ggatagaatc tcctgtgaaa agaagttctt actataaaac
     9541 aaacatgagc ttattagact atatcatcct gttttccgtt actatttttt tcttaagtat
     9601 tttgatttga ggtgaaagaa ttgtttgaat ttaacaatgt ttctttttca tataaggatg
     9661 ttaaggaaca agcgcttgtt gaccttaatt tttcaataaa aaaaggcagt cttacattga
     9721 ttactggttc ttctgggagt gggaaatcca cactattaaa gttaatgaat ggacttattc
     9781 caagacttta tgacggaaaa ttggttgggg aaattttatt tgaaaataac tcgctacaat
     9841 ccttaacaca agaaagtata tcaaaaaata ttggctacgt aagtcaaaat cctagaggac
     9901 aattctttac aacaaataca acgagtgagt tagtattttc gatggaaaac tttggattta
     9961 caaaagagca tatggataaa agaatcaatg aattagctga attattagat ctttcaaata
    10021 tattagataa aaacattttt tcgctatcaa gtggtgaacg gcagaaaatt tctattggat
    10081 gtagtttatc gctaaactct aaattaatta tattggatga accgtcttca aatttggact
    10141 tcaaagcgac taagaaactt gctgacttac tttgtgattt aaaaaataaa ggatttacta
    10201 ttgttatagc tgaacatcgt ttttactatt tgaaagaatt aattgatcaa gttctatttg
    10261 ttaaaaatag aactgtaaaa actacaacta ttcagttgtt aaaaactgaa aacaataacg
    10321 cattaaggaa atttgacatt tttaatagtt cgattgaagt tccctatatc gaaaaggtaa
    10381 gtgcagatgt attaaaagtt gaagaaatta cttatgaaaa tattctaaaa gacataaatt
    10441 ttgatgtcaa agttggagat gttattggtt tggtagggaa aaacggtgtg gggaaaacaa
    10501 ccttactaag aatactcatg aagattattc gccctaccaa aggaaagata atcgaaaata
    10561 aaaaaaattt gtcatcgccg tttttagtaa tgcaagaaat ggattaccaa tttttcacag
    10621 aatcagtgag gagcgagtta gccctgggga atgaaatagt tagtaaagat cagcaagaaa
    10681 aaattttaaa aaaaatggaa ctttataaaa tgaaagatca attaccgttt gatttatccg
    10741 gtggagaaaa acaaagatta ttggtttcta tagcttcatt gtcaaaaact aatttatttc
    10801 tatttgatga acctacaagt gggttggatt atatcaatat ggaaaggatt gctgacctta
    10861 ttaaagactt aaaagaaaag ggtgcagtag ttatagccac tcatgatccg gaatttcttt
    10921 ataaaacatg caatagaatt atttatttaa aaaatggtgg aataaaaaaa gatataaaac
    10981 ttagagtaga agattcaact ttgatagaac agatttttaa tgacataacg aaatagaatt
    11041 gaggagaata tatatgaaaa caaaagatta tatttttatt ggtttagcta cagttatttc
    11101 cctagtaatt tacttttttt caataatggt tagtagtatt ggtggggcgt ttggtcacag
    11161 catatcccca gggatttttg gattattatc tggaatcata tttgtttata ttagctataa
    11221 ctaccctaga aaaggaattt ttactattta cacaattgtt ttattgttat tttttacgtt
    11281 aatgggcggg gcgtacttac cttggtttat cagttcgatt agtatggcga tcttagctga
    11341 tattattcta tctgtatttg gatatgatcg agcaattcct caagttgtgt cttgggttct
    11401 aatgcaacta ggcagtgctg ccggacagtg gattcccata tggttcttta cagatcgttt
    11461 tcgtcaagac tggatagata gaggacaatc tgctgcaaca atggatgcaa tgatccacta
    11521 cgcggtgggt atatggggaa ttatttcagt gttagttgta gcaagtttat ctatgattgg
    11581 tgtattgatt ggaagaaaag tcttaaaaaa atacaaaaaa tagaacgtag tttaccttct
    11641 atctgtttgt acatctctaa tttacgctat tacggaaagc attaacagtt gtaaacgaat
    11701 aagagaagaa attgaatgcg ggggaggaaa aaatgaaagt atacaaaaaa ttatttagtt
    11761 atgtccctaa aatgaaatac tacggatggt tagccacttt attttctgat ttttctgtcg
    11821 tattaacagt ctatggatat tacagtattt acaaatttct tcagtctcta attattgtag
    11881 gagatgaata tttagctaag agttatgcgg ttcaaactgt tgcttggtta actttaggag
    11941 ctttatttta tattttttct ggcttatttt ctcatatcct tgggtttcgc ttagagacta
    12001 atttgagaaa aaagggaatt agcggtttaa cagaagcaag ttttcgtttt tttgatttaa
    12061 attcttcggg atatattcga aagacaattg atgacaacgc ggctaaaaca catatggcaa
    12121 ttgctcactt gattccagat agtgctcaag ccatagtaac acctgtttta tcagttgcct
    12181 taggcttttt cattaacttt aaaattggat tggttctaat cgccatgtta gtaattggtg
    12241 tattcttgtt aaagaaaatg acagagaata caaattttat tcaaaaatat caagaatcgt
    12301 tagatgtgtt aagctctgaa acggtagaat atatccgcgg aattcaagtt ataaagatat
    12361 ttggtgctaa agtaacatca tttaaagcct tgaataaagc aattaacgat tatgctaagt
    12421 ttgcatataa gtattctttg agtgggaaaa caccttttgt tctttttcaa tggctatttt
    12481 ttggcatagt ttgcattttg atagttccta taaccttttt tataacttct cttaccactc
    12541 ccaagatctt agctgttgac ttgttgatgt tgtttttttt aagtggaatt atttttgttt
    12601 ctttcatgag aataatgtat gtaagtatgc acttgtttga agcaagatat gcagtcgaaa
    12661 cactagactc cctttattta gaaatgaagc aagaccaact aaaatatggg aacgagacac
    12721 agattaaaaa ttgtgacatt gcatttgaaa atgtcagctt ttcatacaaa gaagagtgtg
    12781 ttttagaaaa tttcaactta gttttagaac aaaaaagaat ttatgcatta gtaggaaaat
    12841 caggaagcgg aaagagtacg atagctaaac taatttcagg tttctacaat gtggataagg
    12901 gacagattaa aattggtggt aagcctttaa atcactattc aaaagacaca attattaaca
    12961 caattgcttt tgtctttcaa gatacgaaac tattcaatca gagtatttac gataacgttt
    13021 ctatagcaaa tcctcaagcg ccagcaagta aagtatttga cgcgctaaga ttagccggtt
    13081 gcgaatcgat tttagaaaaa tttcccgaaa gagagcatac cttaattggt agtaaaggag
    13141 tttatctgtc aggaggagaa aaacaacgta tagctattgc cagagcaatt ttaaaagatg
    13201 caaagattgt tattatggat gaagccagtg catctattga tccagataat gagtatgaac
    13261 tacaaaaggc ttttcaatat ttgatgaaag aaaaaacagt tattatgata gctcatcgat
    13321 tatccagcat taaatctgta gatgaaattc ttgtcttgtc agaaggaaaa atagtagaac
    13381 gtggaaatca taatgaatta atggctaaac atcgagtgta taaacaattg gtcactaatt
    13441 atgagaatgc aaatgattgg aggcttgaca atgaaaaatt tttataaaaa aaaatttgct
    13501 ttaactgatc agggcgcaga agctttaact aaagcttcta tttctagttt ttttgtatac
    13561 tgcattaata tggtaccagc attcattata atgatgctga tagacgaatt agttttagaa
    13621 aatgcaaagc cccgttggct ttattttgca gtatcgtttg ttactttgct tttcatgtat
    13681 tggttgttag atagggagta tgaaaactta tataatagta cttataaaga aagtgcgcat
    13741 ttaagagtgc aaattgcaga cgatttgtca aatttaccat tatcctattt ttcaaaacat
    13801 aatttatcag atttatctca aactatcatg tctgacgttg aaggtattga gcatgcgatg
    13861 agtcatgcaa tacctaaagc cggcggtatg gctctgtttt tcccttttat ttcagtgatg
    13921 cttttggttg gtaatgtcaa aatgggatta gctgttattt tgccaacgtt atttagtttt
    13981 gtcttaatct tgttatcaaa gaaatcccaa acgaaagcca atactaaata ttacgatact
    14041 ttgagagaaa actcggaaga atttcaagaa actattgaat tgcagcaaga gattaatagc
    14101 tttaatctat ctaaaaaagt tcaagacaga cttttcaaaa aaatggaaga gagtgaaagg
    14161 attcatttaa aggtagaatt aagtactttt tcagtcatgg ccttatcctc tattttctca
    14221 tatgttagtt tagcagtagt cattctagta ggtgttcact tactgttaac gggtgaagtg
    14281 actatactct acgtcgttgg ttacttacta gccgcaataa aaataaagga ttccttcgat
    14341 tctatgaaag aagcagtgct tgaaatattt tatttagctc ctaaaataca gcgaattaga
    14401 gctatgaagg aaaccagtat ccaagaggga tcagatagtc ctctgaaatc ttttgacatt
    14461 gagttgcgag atgtctcttt ctcatatgat aacaataccc caattttaga tcatatctct
    14521 tttacggcta aacaaggaga agtgactgcg ttagtaggtg ctagtggttc cgggaaaact
    14581 agtatcttga aattagtgtc tagactgtat gattatgatg aaggctgtat tttaattgat
    14641 gggtatgata ttaaacgtgt atctccagct tctttgttta gcaaaatcgc tattgttttt
    14701 caagaggtaa ctttgtttaa tacaagtatt ttggaaaata tacgtattgg aaatagtcaa
    14761 gcgtcagatg aggaagtgaa aaaagcagct cgtttagcta actgcgaaga ctttattgaa
    14821 aaactacctg acggttatca tacattggtc ggagaaaacg gaagttcact ttctggagga
    14881 gagcgacaac gcctatccat agcacgggct tttttgaaaa atgcgccaat tttaatttta
    14941 gatgaaatta ctgccagttt agatgcggaa aatgaaaaac gtattcagga aagtttaaat
    15001 cgtttaatac aagataagac agtactaatt atatcgcatc gcttgaagtc tattgaaaag
    15061 gtaaataaaa ttgtagtgat ggatcaagga aaactagttg atcaaggtac tcataatgag
    15121 ttgtatcgaa ggtcagaaat atacaaaaat ctcataaaga aaactaaact atctgagaaa
    15181 ttcgtctatg aaaaagaagc tcaatcacga tgatgaaata ggctttaaat taacctacaa
    15241 tttttgggat atagttataa ttctttttca aaaagaataa ttttattaaa cagcatctaa
    15301 ttttgaatac acgaaagaat cagtatgatt gaattagttc acttaatgtc tattcttata
    15361 atataaggaa taatcaaagt tttaaaaaat tggcgataaa ccagttaaac ataaaagatt
    15421 gaataaaaag ttatagtttc tttcatattg ccgttttaag tagaggaaaa gctacttaaa
    15481 acggtatttg tgtttgattg gactaataaa agtgaattct tatttttcag agcctaaact
    15541 gcatattctt ttaaatgatt tgattatctc tatgtaccga taaattcgtt ttcaaatagt
    15601 tatctattca tatgagtttt atttcatgtt atttattgtt gacaagagca actcttgttg
    15661 gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag gtgtctaaat caaaataaaa
    15721 ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa acaaatggcg
    15781 atgattgaaa agtatgcaga taaacgtcag gaattaaaag ccgcaggaga tcgcaccgct
    15841 ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga tcagacggat
    15901 ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc gtattaagtt tagagaactt
    15961 gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga ggaaatatca
    16021 tggcagtacc agcaagaaaa acatctaagg cgaagaaacg gttaagaaga actcatcaaa
    16081 ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg tgattataga cgtagccatc
    16141 atgtatcttt aaaagggtac tataatggaa agaaagtaat taaaggagct tcaaaatagt
    16201 ttaatatata acttttagga gggaatcaaa atggaaatta tttacccacc tttagttgaa
    16261 gaaggattaa agtattatct tgaaacgacc cagcaatcgc tagataaatg cactttttat
    16321 cgttcgatgg tagagagagg aattattact gaaacgggtt taccgaccca acaggcaatt
    16381 gaaaatggat tagtcaaaga ttactatgag gaccaaggct tatcatttga tgatttttta
    16441 agaatctatc caatttttga agagtatgac gaggagttat ttcaatgtat cgatggttat
    16501 tgggagatac ctgttgatat gaaagaaaat ttagtttcac aattggaatc tggcgagtta
    16561 acttttgaag atgcacaaca aattcaagca tatcttgaag atcgataagt attgaaggag
    16621 gaagatttat gcgtcaaaat attattttgg aatgtgtcga aactggagaa cgtctgtatc
    16681 tcaccagcaa aaataaacgg aataaccctg aacggttaga attaaaaaag tattcgccaa
    16741 aattgcgcag acgggcaatt tttaaagaag tgaaatagct aagtggtaaa aaggtgacaa
    16801 aatgggcata ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa caacgctaat
    16861 caatcaagcc ttgcaagtct caccctaccc cagggaagaa attattataa ttgaaaatga
    16921 gtttggtgaa gttgggattg atcacaaatt attagttcat agcgaggaac gggtgttaca
    16981 actaaataat ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa tattgatttc
    17041 tttactggaa acaatagaag aacatcaaca accaatttca caaatcatca ttgaaactac
    17101 aggtatagca gatccccaac caatcattca aacactttta acagcgcctc aactaagagg
    17161 aagagtatat atcgatagtt tattgactgt tattgatagt gacagttttg aacttattga
    17221 aagagaacca caagccctta agcaacttgt catggcagat cgcatttttg tgtctagaaa
    17281 acaaaacttg aatttatctg cactaaaaaa attacagaga gaaatcaaaa gtattaaccc
    17341 tttatccgat attcgaatgt tcacacatag tgaaacgatt caaaaaaagg atttttataa
    17401 tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat tcatcagaaa atcactataa
    17461 tggcaaatac tatcatcaac accatcaagt acatgagttt aaatcaattc tcttaacttc
    17521 agatcaagac ataaaaaaag attttttact ccaatggatt gactggctga ttttaaccaa
    17581 ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta caagagcagg attttatagt
    17641 tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac accactcgac aaaaagacca
    17701 aaccactatt attctaatcg gaaaaatgtt ggatgaagaa aggatcaaac agagttttga
    17761 taaattactg gcggaaagca aataatgata agtagtgatt gggggtaatt tctaatggag
    17821 aaaacggata tatctagtgc atatcgacga cttaaaagcc cgaatattaa aactagaaaa
    17881 agggcattaa aaattatcaa agaacataaa cgaaataaaa tgaagaaatt agcataatta
    17941 aggaaggaaa aagaatgaag aaaatcagtg taggacttat tgggatagca gctctaggat
    18001 tattgggagc ttgctccagc actaatgatg caaaagtatc gaatgataaa gatggaaaat
    18061 tagaaattgt aacaacattc tatccaatgt atgatttcac caaaaacatc gtcggcgatg
    18121 aagcgaacgt ggatttaatg gttccagcgg gatcagaacc tcatgactat gagccctcag
    18181 ctaaggatat ggcaaaagct catgatgctg atgtttttgt atatcataac gaaaacatgg
    18241 aatcatgggt accaaaagct aaagaaagtt ggaagaaagc tgggcctaat gtagtagaag
    18301 gaacaaaaga catgatttta ttgccaggta gtgaagaaga agatcatgat cacggagaag
    18361 aagatcacca tcatgaacta gatcctcata cgtgggtttc acccaaaatg gcaattaagg
    18421 aagtttcaaa tatcaaggat cagttagtta aattgtatcc gaaaaaagca aaagtatttg
    18481 agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac tataccacta
    18541 gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca cgcagccttt ggatatttag
    18601 ctttggatta tggattgaaa caagttccta ttgctggact aagtcctgaa gaagagccat
    18661 cttctggtcg attagctgaa ttaaaagagt atgttaaaaa aaataagatc aattatattt
    18721 actttgaaaa gaatgctaat gataaaattg caaaaacttt ggccaacgaa gctgggataa
    18781 aattggaagt actgaatcct cttgaaagtc ttacaaaaga acaaatggat aatggtgaag
    18841 attatgtttc agtaatggaa gacaatctaa aagcgttaga aaaaacaact atggttgctg
    18901 gtgaagaggt agttccagaa aaagaagcga aggatgaaaa gacagttgct aatgggtact
    18961 ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta tacagacgaa tggcaatctg
    19021 tttatccgtt gttaaaagat ggaattttgg atgaagtttt cgattacaaa gcaaaaatca
    19081 ataaagacat gacagctgcc gagtacaaag attattatac tactggctat aagacagata
    19141 ttgatactat caacattaaa gacaacacca ttgattttgt agtcaacggt gaacatcatc
    19201 aatacacata caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga aaccgtggtg
    19261 ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg ttttaaatat attcaattta
    19321 gtgatcatgg aattgcacca agtaaggcag agcatttcca tattttcttt ggtggagaaa
    19381 gtcaagaaaa actctataat gaattgcata attggcctac tttctatcca gcttcattgt
    19441 ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa ttcaattttt ggaggaaata
    19501 catggtaaga aaagtattat tgtctatgga ttccaagaat attgatgctt atcggcgtta
    19561 tcgatttgca aagcaaatga gattaaatgg gagtacatta gctgatattg ctgcaacttt
    19621 acatgttagt attgacacag ttcatagagt attaaaacgg gatcctgatt gttgttttat
    19681 caaggtcaat actggaagaa gtagacgttt agcaaaacgg cataatagct tggccaatta
    19741 attgataata ccagagtaga tcatttttcc cttatttgga tttgatctgc tttttattat
    19801 gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga agacttcata aggtgttctc
    19861 caatctaaac actttctagg tctgttattc agtttttcaa tcatctgatt tatcgttgag
    19921 ttatctaccg tagctatctc tttcgatttt ggcaagtatt ctcgaatgag tctattcgta
    19981 ttttaatttg ttcattgttg ccaagggcat gaggatctgg aaaataaaag ggcatattat
    20041 tcaatgcttc tgtaactgcg aaatgattgg agaattcttt tcctctatct ggagtgatcg
    20101 ttttgaccgc ctcatttgga atactgctta aaagttcaat cattttctcg ctcacataag
    20161 cggctttctt tctggggacc ttctttcctg ctaaaaggaa gcatgatttt ctgtctgtca
    20221 aagtcactaa acaagtacca ccagttttgc cagctacaat atcggctttc caatgaccaa
    20281 aatcacttct atcatctgcg gattttggac ggccatggat cgaatgagta aggcaaatct
    20341 ttccacgaga ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg tgcaatgaac
    20401 ggatcaggcc tttattgccg tgtgagaggg gttctgtatc gaaaagatca ttgcaaattg
    20461 cacgttagat cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc aaacgattcg
    20521 agatttcctc tggcgaccac tgaaaatcga caaataaatg acgaacaatt gaccaaactc
    20581 acggatcatt tagaatgtga taacgtccgc atttttttct tcgataagca taattttttt
    20641 ccgctttgga aggtgaatag tttttttctt gtttcttctc agttcatgta aaatagtaaa
    20701 cgggctatgc tgaatgtcgt tgccaattat actaatagaa aatccttctt cgtgaagcag
    20761 aaagattctt tctcgttcga gtatagtaag atgacgatac tggctcataa ttattttcct
    20821 tctaggagtg ttctcgacaa acttatacta gttggatatc gattatgact catttttatg
    20881 tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa aaaaatacct
    20941 attacaatct taactggcta tcttggttca ggaaaaacga tacttctaaa tcatattctg
    21001 tacggtgatc acggaaagga aattgctgtc attgtaaatg acatagggga agtaaatgtt
    21061 gatggggtat tagtcgacaa gaaaggtttt aaaagaactg gagaaaaact tgtttcgatg
    21121 ccgaatggat gcatttgttg tacattacgc gaagatttga ttgttgaact agatcaatta
    21181 gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt caggaatcag caaaccaatt
    21241 ccaattattc aagcaattat attagctaaa aaccctttag acattgattt gacccaacga
    21301 ttagagattg gtgccattat tatagtagtg gatgtaaaac gtttttggga taatttcgaa
    21361 acgggtgaaa agattcagga aagaaaagct gaaggtaatg tagttgaaga agaacaggat
    21421 attcgtggct tgttaattga tcaattcgag tgttgtaaca tattactttt aaataatagt
    21481 gacttagtgt caaccaagac gatttacaaa attaacggtc taattgctaa gttacagtcc
    21541 aatgccaaaa taattaggac aattaaaggt gaagtagact tatcgaaaat tctaaataca
    21601 aatcttttta atatagatga gacttcctat tcagtgggct ggttcaaaga gttagagctt
    21661 ggctatcaaa atcatattcc tgaaacagaa gaatatggta tctcaagttt tatctatcga
    21721 aatcggttac tccttgacgc acaaaaattt gcccatctta cggataaaaa ttttcctggc
    21781 aatattacaa gagcaaaagg gtttctttgg tttgcccaac atagtgattt ttcgacgatc
    21841 tttgaaacat cgggtagcta taaaagtatt gaatcattaa attattgggt agctagttta
    21901 cctaaaattg agtgtcaaat gattatacaa aaaaatcccg aattgaagga agcctggcat
    21961 ccgttgtttg gtgaacgcat taataaacta gtttttattg gtatgaagat ggatcaacaa
    22021 tgtattaagg aacaacttga gggttgttta actgattctc ttaatgcaga taatttccga
    22081 aaaatttcac catttccatg gaaaaagtct aatatgttta gccagatatc gttaatgaaa
    22141 tttttaagaa aagctggtgt caagataggc gatgtcataa ttattggcat tttgattatt
    22201 acctctttta ttcccttatt tgtattctct atagaaaagg agaatgaaac aactgaagat
    22261 gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa tggatcgctt cgatttggat
    22321 aagatcaatc ataaactcgt gaattatcat cctgcgaagg gacaatataa tattgttgag
    22381 attaaagatg gaagaattcg agttaaagaa gataatagtc ctgatcaaat cgcggttaga
    22441 actggatgga tctcaaaacc cggtcaaacg agtatttgtt taccccataa gttagttatt
    22501 agtattgaga aaaaagaaag cacagattac tacatttact aatctggaaa tagatgagga
    22561 ggtttttaat aagaaaaaga tattattatt aataatagca ggggctttat taagtgtgtt
    22621 aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc acaagtatct ttaacgggac
    22681 ctgcaatggt ttagttaaca gtcggttaga ttacttattt tggctcttcg tcaatgagtg
    22741 ttggtgaaga aattcaatag aataatcgag atactcacac ggctagacag ctatcttgtc
    22801 tagccgtgtt gttgttctgg ctttagtcag ttgggtagta ttcttagttt tcttaaaata
    22861 ttttcctcga tataaaaaga ttcgttctcg gtagttttga aagtttctat agccataggc
    22921 aattcgtttt agtactttga tatggttatt caaacattct atagggccgt tggaataggg
    22981 aacgcgcaac gctcgtttga tttctgtttg gtatttcttg aaggtcgtga acactggttg
    23041 gtagtgctct ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt caggaacatc
    23101 ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg tagcctcgct ttaaggaatc
    23161 atcataagat aatagccgat cgactatctc tgcttctgtc agatgatctc gaaatgacgg
    23221 acgccagtag cgatgctgac ctttcaattc ccacgcattc ttttgaagta gtttccagta
    23281 tttctttaag tgcttcgacc gacgatcccc ttttttaaag gtcttcatta cttgaattcg
    23341 gtggttggta aaagcccgac caatgtgttg gctaatatga aagcgatcgg cgacgattcg
    23401 ggcattcgga aaacactctt tcaccaatgt gcgatagggt gtatagaaat ctgaaaccac
    23461 tagacgcact tgttcacgga cttttatagg gtagcgcaag aaatagtcgc gtaaaggatt
    23521 gagttgccga ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca tcatgacaaa
    23581 gctcatggct cccgagacat ttttcactga tttgaattca tcgaaacaca ccacttctgg
    23641 taacagctgg ctcaaagaaa tggttttagg ccgaaggctg cgaagtattc gaatgacagt
    23701 ggtcggagaa atgtgcttca tacgtgcgat caaagacata gaaagaggtt cgcttaatag
    23761 ctcaagaatg gcttgtttga ctctgcgagc aatggagcag cgacgatcac taactgaatc
    23821 ttctgctaaa aaagtctttc cacagtgttt acatttgaag cgctgtttgt taatccgcag
    23881 aatggtcttg tagttactga catcattcaa taagatggat acttttttcc agccctattt
    23941 aatgatcgtt tgttcatttt gatagtggcg atgaaagcat tcctccggtt gataggtcaa
    24001 tgtgccatca aaaacaaaac atgtttcgtc attgattcgc tctttgcgaa aggcatcttc
    24061 atgaaagata agatttaggt ctagaatatc tagtaactct ttggtaaact ctgaatagga
    24121 catcttgaaa cactcctttg aatggttttc gtcgacttta ttctaaagga ttttaaggtg
    24181 tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt caccaacact
    24241 agatattata gatcccttat tttcttcgct tcaaattgtc aaggagtcat cataaatttt
    24301 gtatggattc ttttgtttgg gtagtagtct tctaaataat cttggattaa caccttcatc
    24361 tctattttac ttttatattt gttggagtcg attaattctc atttgattga tcgatgaaac
    24421 gcttcaatta ctgcattgtc aacaggagtc ccagtttggc tcactgaatg tgtgatgtgg
    24481 tggtcgttca acagctcttg ataaataaaa gaccgatgct ggctacctct atcggaatga
    24541 attaccgtag gcaattctcc ttaataaatt tgtaatggac ggacgacaat ctcttttttc
    24601 atgtgtgaat ctaccatata gtaacgtaga atacgagttg ctaagtcaat ataggtggac
    24661 aaatagaggc gaccgtcatt acatggaatg taagtaatat ccatcaccca aacagcatca
    24721 atttggttct gttcaaatta tcgattcaac atttttttac catttctttg attgtttttt
    24781 ctctaccatg agaagatttg cgtcgatgaa atcctcttgt gcataaattc atttcacgca
    24841 taagaacagc tacaacacgt ttattgattt cgattccttc atcgtaaagt ttttgagtga
    24901 tccctggaga tccgtatcga tctttgtgtt cgtagaacac tcgtcttcct cttttagtcg
    24961 tttattttcc tgttcaagaa tgtagaaatc tgctttatca ggtaatcttt ttccttgtcc
    25021 agcaaaagca tttttcccgt atcgacgata ttctgaaacc catcgggaaa ttgttggacg
    25081 agagatattg tattcttcag caattttctt ggtagttact tttttctcaa ggatatcctg
    25141 tgaaatcttt agtttaaatt tacgatcata tgtttttctc aatagagttc attccttccg
    25201 gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca acttagtatc agggtttgtc
    25261 aactaaactg tggaagttga tagataagag tttttaacaa ccaaaattct cttgactatt
    25321 tttgaaatca gacaaatttt gattggacac atagttgaat aaatctaccg tcgttacgga
    25381 aggtaaatcg catgcttttc aattctagag gagaagtcct cgttgaagta ccatagccca
    25441 gttctggatt cgacctcctg cccacttcat acgtaaataa agtaatttta ggagtgagac
    25501 tgtgacaaaa gttattttta agctaaaaac gaattaaaaa acgttgataa ttaaaatcat
    25561 tcgttttaga ttctatgaat cattatagaa cgctgtaaaa tcgatacttt ataattataa
    25621 agaagatagt ttcaaattta ttgttcgaaa tttgaaacta tctttttact tatgtcacag
    25681 actcccttag ttgagttatt ctttaatttc gaatttcgag atagctagat atattgcaag
    25741 catacattct tgataacaat cttgatcata ttttccagtt ctccaggatt gcttatgaat
    25801 caaagagcta aatcttttga gaattgcctc aacagcttct tcatttccag cctttgctaa
    25861 aatgaaatct tcttttaaag aattcataaa tctaccttct tcccatggac ttataatcta
    25921 tttctttggg aaggaggaga acgagcaaag aagcatacaa aattagttat tatgcagata
    25981 ttaaaccagc attttttgaa tttttttgta aagtcgatgt tttaaatttg tcgcaccctg
    26041 tcttgtaatc cccagaacct ttccaatttc tttatcagtt ttatcaaaga taaatttttc
    26101 gataagaaaa atttgttcag gttctttcaa caaaggaagg atttttgcta attttatatc
    26161 ttcaacaagg agagtcacat ttaaaacatt aatcggttgt ttaaatttta tagtatcttt
    26221 agtaaacaaa tatggtggga gttgatcaag ttgttcatgt ttttgaattt cttcttgttt
    26281 tttataataa gtattagcgg tgttcaaaat gaccttttca aagaatcgaa tcaactgggc
    26341 atttacgcgt tcgtcgttat ctgacatatt gcacacctcc tccattaaga tttaaaggga
    26401 aaagaggcaa aaaacacgaa aagaagctaa tgcaattatg ctgcgctgcg aataacttct
    26461 ataagctcac atgcattgat aggactgctg atgtttattt tcaaaaatgt ttttttagtt
    26521 gataatggat agttatcttt atacttagaa aagaaaattc atatatcttc ttaaatatca
    26581 aacatgtcct tatggctaag aaaatgtctt gagcttttta cgtataataa atcttttcta
    26641 aaacctcctt ttggataata ttcttattcg tgtatagatt gtatttttta tttcttcccc
    26701 cttcataatt aacaactaga aaagagttcg aatagacacg tatttgaacg aaaattttta
    26761 ttggattccc aaattggaat atttagaaac gaaaaattcc tattatctgt atagtgctta
    26821 gataataacg gatcataata attattgtct ttgaactaga tatatgtaaa tagtattcat
    26881 catgatgaaa aatgatgagt tctcggtgat aaaatgtact ttttacggtg gaatatacat
    26941 gaaagcgata agtggatatt ttataatgaa gtaagttagc ttattttgac agaggaatag
    27001 tattttagat aacgtataaa atactattta atgatgtaga aaggaaagag agaaacaaaa
    27061 gtgttttcag ataaagaagt cttattaaag tctcttcatg tttgtttaaa gttacctatc
    27121 gtggttttta attccgaatt cagtattata aaagagtatc gctcagatag aactacttat
    27181 tttttttatg attataaaaa gttactaact gataatatta aaaaaactaa agatttccat
    27241 ttctttactg gtgattttaa tgagatgttt cttctataca tgtttaaaac cagatattac
    27301 ttatttggtc catttagagc aaataatatt gataaagttt tttttaaact aaaaatgaac
    27361 aatttgaatg ttgcaatggc tgatagagaa cgactctata attcattgca aaatttgact
    27421 ttgtattctt taggagacat tcgagatatt ctgattttag ttcattattt ttttactgga
    27481 aaaatagaag atctattcca tgaaccgtta attgaatata cagaaaatct aagtaaaact
    27541 atcgagcaaa tcaaaataga taatctgtta tcacaaaatt acgatccaga aatatatctg
    27601 tttctttatg aaaataaaat actggaatat gttaaaaatg gagatattcg aaatttagaa
    27661 aacatggttt ttaatctgag taatggaata attccaagtg ttagtggaga tacgattcgg
    27721 tctgaaaaaa attattctat cattgttttt gaaaagttag cacaaacgtc tataacatta
    27781 ggaatggata taatagaagc gtatcaaagt agagatgctt tgatacaaga aaacgaatta
    27841 gctgtatcgt tacctgaggt cttaaaagtg agggattctg ggattgttta ttatactaag
    27901 gaaatcggaa aaactaagat agagcatctt tctcccttaa tttcttctgt cgttcaattc
    27961 atcggcttaa atatctataa aagaattact gtaaaggaaa tagctaatta tttttctgta
    28021 agtgaaacta aattaagaga aagctttaag aatgaaatgc atattacgat ttataattat
    28081 atctctaagc gaaaaatttc tacggctaaa ataatgttga aatcaaacca tactatcagt
    28141 gaagtttctt taggtttagg attttcagat tcttcacact tttcgagagt attcaaaaag
    28201 tacgcaggtg tatctcctaa gcaatatcaa ttaggtttag tggacaattt caacaacatt
    28261 agctgagagg ttttatttta ttatgagact atatcattta cgtagtttgg aggagttgag
    28321 gaaattggat tttaacaaaa aatttttttg ggaaatttct gatttgagtt gtttcgtaga
    28381 atctgagcgt ctaagtaatg gtaataatag tgtgttaaat gaatatgaca aagtagtaat
    28441 taataaagta aaaagtttat gtgaagaagc tagagaatct atcagtcata ttaatggtag
    28501 caattttaaa caaacattat ttaatctgat taagctagat gctaaaatat caagttattt
    28561 attctttttg atacatgatg aatttatgga ttatcaaaaa ttggatcagt taattgaaag
    28621 agaaagctgg gaagattatt acgtgggtct aactttttca gaaaagttaa acaactacaa
    28681 gttaatagat tataagtatt tgagtgaaag caattaatta ttcatgtaga gtactagaca
    28741 ctttaaatat ggacaattca attataaaga aaaagaatca ataatttaat gaggctgtga
    28801 caaatgtcat tttttgtttc gctaaaaacg agtgaaaaaa tgttgataac tccaatcatt
    28861 cgtttaagat tctaggaatc attcagaaat gctgtgaaac cgatacttta taattataaa
    28921 gaaggtgatt tcaaattccg aagaaggaat ttgaagtcac ctttttactt atgtcacagt
    28981 ctcattcagt aatgcgggat aaggctcctt attccgttat ttgaatactc tgaagtttaa
    29041 tctaagaaag ggtaatattt tgaaaacatg cttcatcgca tagaaatgaa cttaatttca
    29101 gaaagaatac atacatatct ttttatatgt tttcagctgc tgaatggtta aaaagcacaa
    29161 atatccgaaa aaaacatgaa gaaatgtact ttttttgttg taatgttcat gaaattctct
    29221 aagtaaatta gttaacataa ggttagtaag attcctattc tgtgaataat acgtatcaga
    29281 tttatctttt attcttacta ggaaagtaat tattaaggga gttgatttga ataatggtta
    29341 gcaagaataa taagagagta tttctagaaa aaacaaagaa acgagtattg aagtacagta
    29401 tcaaaaaatt aagtgttggg gttgcatctg ttttgattgg tgttggtctt gtcttgggaa
    29461 caactgaact agttaaagca caagatgaaa tttctccaag cagcccactt gaaacagcga
    29521 taagctccgt acaagtaggt gacaaagtag cttcggggaa tacttttcag gagaatcctg
    29581 gttatacgaa aaattataac ttttcggatt tacaatttag tcctcaagaa ctaactggag
    29641 acacactaaa aggcaataca attggatttg aagtttacgg aaaacataat attgcagcat
    29701 caactaaaaa ctgggaaatc cgtcttcaat tagatgaacg attagctaaa tatgtagaaa
    29761 aaattcaggt cgatccaaag aaaggaattg gatcttctag acggaccttt gtaagaatta
    29821 atgattcgct gggtagacct acaaatattt ggaaggtcaa ttatattcgt gcaagtgatg
    29881 gattatttgc tggggcagaa acaaccgata cgcaaaccgc tcctaatggg gtaattacgt
    29941 ttgaaaagag cctagatgaa atttttaaag agataggcat agacaatctc aagaccgatc
    30001 gtttaatgta tcgaatctac ttggtaagtc atcaagacga tgataaaatt gtaccgggta
    30061 ttgatagtac gggctacttt ttaacagatt cagatgattt ctataatagc ttagatgtat
    30121 ctgaaaataa tcctgaccag tttaaacatg gatcagtaag tgcaaaatat gagaaaccta
    30181 atactcaaac aaaagatggt tcaggatcca caggtgctaa tggagctata atcttagatc
    30241 ataagttaac gaaaaattat aatttctcat attcatcttc cgcaaagggc acgccttggt
    30301 atgctaatta caaaattgat gaacgattag ttccatatgt agcgggtata cagatgcaca
    30361 tggttcaggc tgacaaagta acatatgatg tttcgtttga atccggaaca aaagtagctg
    30421 atttagcaat cgagcgacgg gaaggtcatg aaaattatgg cataggttca ataactaata
    30481 atgatttaac taaacttatc gattttgcta atgcaagtcc acgtccagtc gttattcgat
    30541 atgttttaca attgactaag ccattagatg aaatcttaga agatatgaaa gcaacagcac
    30601 aagttgaaga aaataaacca tttggtgaag atttcatctt tgattcttgg ttgtcggata
    30661 cgaataaaaa attaatccag aacacttatg gaacaggtta ttattatttg caagatattg
    30721 atggtgatgg aaaccctgac gataaagaag agagcggaga cacgaatcca tatatcggga
    30781 aacctgaatt agaagaagta tatgatgttg acacaacagt taaggggaaa gtattcatcc
    30841 acgagttagc gggaacaggt cacaaagccc aacttgttga taaagaaggt actgtattag
    30901 cagaaaaaac tatcgctcca aatgaaaaag atggggctcc aatttcagat actgtagaat
    30961 ttgaatttac gggtgtagat tcaagtaaat taatcgcgaa agatgaatta aaaatccaaa
    31021 tcgtttctcc aggttttgat aaaccagaag aaggttcaac cgttattaag gaatcaccaa
    31081 aagcggttga taaacaaacc gtggtagttg gatttaaacc agatgctaaa gaatcaattc
    31141 ggaataataa aaacttacct gaagatgcag agtattcatg gaaaacagag cctgatactt
    31201 ctaacgttac tgatagtacg aaaggtattg taactgttaa gatcggaaat cgaactttcg
    31261 acgtggatgt agagtttgct gtaaaagctt ctcaagctat ggaaaatgat gcaacatacg
    31321 tacctataac aacaactcca gaaacgacag ttcaaagtgg aaaacctaca tttgataaac
    31381 cagatgttcc tttagctaaa gatgcctttt caatttcaga tgtctataat aaggacttcg
    31441 gtaatgcaag tgttgatgca aacactggta ttgttacatt cactccagct aaaggtgtag
    31501 gagaatcgga gccgattact ggaacaattc ctattaaaat tgtttaccaa gatggttctg
    31561 taggcacgac cgatttagca gtaactgtaa gtaaagatat ttatgaaaat ccaggagaaa
    31621 acattcctgc aggctaccac aaagtaacct tcaccgcagg
//
LOCUS       DACEMS010000020        30465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEMS010000020 DACEMS010000000
VERSION     DACEMS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.020"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..554)
                     /locus_tag="IW735_000831"
     CDS             complement(<1..554)
                     /locus_tag="IW735_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1043848.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(680..2608)
                     /locus_tag="IW735_000832"
     CDS             complement(680..2608)
                     /locus_tag="IW735_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1043849.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2914..3630)
                     /locus_tag="IW735_000833"
     CDS             complement(2914..3630)
                     /locus_tag="IW735_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1043850.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3631..5454)
                     /gene="glpO"
                     /locus_tag="IW735_000834"
     CDS             complement(3631..5454)
                     /gene="glpO"
                     /locus_tag="IW735_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1043851.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5468..6964)
                     /gene="glpK"
                     /locus_tag="IW735_000835"
     CDS             complement(5468..6964)
                     /gene="glpK"
                     /locus_tag="IW735_000835"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1043852.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7100..8560)
                     /locus_tag="IW735_000836"
     CDS             complement(7100..8560)
                     /locus_tag="IW735_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1043853.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8550..9845)
                     /locus_tag="IW735_000837"
     CDS             complement(8550..9845)
                     /locus_tag="IW735_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1043854.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10177..10854)
                     /locus_tag="IW735_000838"
     CDS             complement(10177..10854)
                     /locus_tag="IW735_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1043855.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10884..11975)
                     /locus_tag="IW735_000839"
     CDS             complement(10884..11975)
                     /locus_tag="IW735_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1043856.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12076..12510)
                     /locus_tag="IW735_000840"
     CDS             complement(12076..12510)
                     /locus_tag="IW735_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1043857.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13094..14551)
                     /gene="ascB"
                     /locus_tag="IW735_000841"
     CDS             complement(13094..14551)
                     /gene="ascB"
                     /locus_tag="IW735_000841"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1043858.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14548..16443)
                     /locus_tag="IW735_000842"
     CDS             complement(14548..16443)
                     /locus_tag="IW735_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1043859.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16501..18276)
                     /locus_tag="IW735_000843"
     CDS             complement(16501..18276)
                     /locus_tag="IW735_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1043860.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18326..19822)
                     /locus_tag="IW735_000844"
     CDS             complement(18326..19822)
                     /locus_tag="IW735_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1043861.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20016..21638)
                     /locus_tag="IW735_000845"
     CDS             complement(20016..21638)
                     /locus_tag="IW735_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1043862.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21966..23279)
                     /locus_tag="IW735_000846"
     CDS             complement(21966..23279)
                     /locus_tag="IW735_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1043863.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23524..23748)
                     /locus_tag="IW735_000847"
     CDS             complement(23524..23748)
                     /locus_tag="IW735_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043864.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23768..24136)
                     /locus_tag="IW735_000848"
     CDS             complement(23768..24136)
                     /locus_tag="IW735_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1043865.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24332..25042)
                     /locus_tag="IW735_000849"
     CDS             complement(24332..25042)
                     /locus_tag="IW735_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043866.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25060..25557)
                     /locus_tag="IW735_000850"
     CDS             complement(25060..25557)
                     /locus_tag="IW735_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1043867.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25594..26046)
                     /locus_tag="IW735_000851"
     CDS             complement(25594..26046)
                     /locus_tag="IW735_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1043868.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26181..26786)
                     /locus_tag="IW735_000852"
     CDS             complement(26181..26786)
                     /locus_tag="IW735_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1043869.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27236..27856)
                     /locus_tag="IW735_000853"
     CDS             complement(27236..27856)
                     /locus_tag="IW735_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1043870.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27858..28727)
                     /locus_tag="IW735_000854"
     CDS             complement(27858..28727)
                     /locus_tag="IW735_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1043871.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28750..29157)
                     /locus_tag="IW735_000855"
     CDS             complement(28750..29157)
                     /locus_tag="IW735_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1043872.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29176..30465)
                     /locus_tag="IW735_000856"
     CDS             complement(29176..30465)
                     /locus_tag="IW735_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1043873.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8631 a         6627 c         5021 g        10186 t
ORIGIN      
        1 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
       61 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
      121 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
      181 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
      241 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
      301 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
      361 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
      421 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
      481 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
      541 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
      601 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacagc cactgatatt
      661 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
      721 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
      781 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
      841 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
      901 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
      961 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctggcgttct tttttattct
     1021 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
     1081 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
     1141 caatccacaa agcttggctt tctcctgttt ctccaacccg caactgatat ttaggacgaa
     1201 gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt
     1261 tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt
     1321 ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat
     1381 ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat
     1441 gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa
     1501 tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg
     1561 ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc
     1621 cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct
     1681 caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag
     1741 gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg
     1801 tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg
     1861 ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat
     1921 tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga
     1981 taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc
     2041 ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt
     2101 tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca
     2161 aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt
     2221 ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt
     2281 gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga
     2341 tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc
     2401 tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg
     2461 tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc
     2521 gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt
     2581 gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc
     2641 caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg
     2701 tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac
     2761 accaaataaa ctttgtttat tggctattcg acttactgat tagataccag ctacactcac
     2821 aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag caagacacat
     2881 tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat agacacctac
     2941 cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga
     3001 agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc
     3061 agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc
     3121 acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc
     3181 aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat
     3241 agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg
     3301 tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat
     3361 aatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac
     3421 tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc
     3481 gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa
     3541 attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc
     3601 ccccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc
     3661 tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca
     3721 ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg
     3781 ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc
     3841 ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc
     3901 catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata
     3961 agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt
     4021 ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat
     4081 ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc
     4141 ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg
     4201 ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt
     4261 ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc
     4321 acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc
     4381 attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc
     4441 gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata
     4501 atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt
     4561 cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt
     4621 tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg
     4681 gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac
     4741 tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc
     4801 atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc
     4861 aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc
     4921 aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc
     4981 ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc
     5041 atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga
     5101 gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg
     5161 ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac
     5221 tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg
     5281 agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc
     5341 tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa
     5401 ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat
     5461 ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt
     5521 tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc
     5581 ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta
     5641 gcgccgttgt ttccaaattt tctgctcgtt ggatcggtac attcaagata tctgtttgga
     5701 attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga
     5761 ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta
     5821 aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc
     5881 gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct
     5941 catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt
     6001 gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag
     6061 catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata
     6121 attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga
     6181 ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc
     6241 ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg
     6301 agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct
     6361 gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag
     6421 taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca
     6481 tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta
     6541 tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt
     6601 gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa
     6661 ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt
     6721 tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg
     6781 caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt
     6841 gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa
     6901 agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg
     6961 tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta tcatactact
     7021 gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta
     7081 ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt cttcttttca
     7141 atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt
     7201 ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct
     7261 caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa
     7321 tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca
     7381 tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt
     7441 tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat
     7501 gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc
     7561 caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt
     7621 ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat
     7681 ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt
     7741 cgtctactat cgaaaaagaa acaaaagagc tgtaaaacat cattgtttcg tatacattca
     7801 gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc
     7861 ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca
     7921 atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa
     7981 acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt
     8041 gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc
     8101 gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta
     8161 agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg
     8221 tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact
     8281 tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca
     8341 caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa
     8401 tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc
     8461 tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct
     8521 gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt
     8581 gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac
     8641 tgttcaaacg tccatccttc ttttactgca attgtcagtt ggtcaagaag gtggaacatc
     8701 tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg
     8761 agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt
     8821 ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca
     8881 aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt
     8941 gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt
     9001 tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga
     9061 gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct
     9121 tcccacatct gattgtcggg gcgagaatga ttagccagca aaacaagatc atagttctcc
     9181 tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct
     9241 tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca
     9301 ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt
     9361 cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt
     9421 ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg
     9481 tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga
     9541 atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat
     9601 cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc
     9661 gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg
     9721 ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc
     9781 aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg
     9841 ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca
     9901 cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc
     9961 tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga
    10021 gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct
    10081 tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt
    10141 gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac
    10201 tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat
    10261 ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc
    10321 atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact
    10381 gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa
    10441 caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga
    10501 agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc
    10561 ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa
    10621 ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa
    10681 aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat
    10741 cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg
    10801 acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc
    10861 atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg
    10921 cttggcaaat aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca
    10981 cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct
    11041 gcaagttctg catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca
    11101 ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg
    11161 ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct
    11221 agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt
    11281 gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt
    11341 tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag
    11401 ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa
    11461 tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg
    11521 gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt
    11581 tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa
    11641 gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct
    11701 ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg
    11761 agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca
    11821 ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg
    11881 aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct
    11941 gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct
    12001 gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa
    12061 tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc
    12121 taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca
    12181 gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa
    12241 gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc
    12301 aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt
    12361 ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc
    12421 ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc
    12481 atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata
    12541 gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt
    12601 tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct
    12661 ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt
    12721 ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt
    12781 ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca
    12841 gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct
    12901 gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag
    12961 caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg
    13021 gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc
    13081 ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa
    13141 atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga
    13201 tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc
    13261 acgacgtata accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat
    13321 gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt
    13381 ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt
    13441 gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa
    13501 gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg
    13561 gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg
    13621 ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca
    13681 ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag
    13741 ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa
    13801 gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg
    13861 tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat
    13921 ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga
    13981 tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag
    14041 cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca
    14101 aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac
    14161 attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac
    14221 catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag
    14281 caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat
    14341 aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc
    14401 attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag
    14461 ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc
    14521 cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct
    14581 tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac
    14641 aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat
    14701 caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc
    14761 atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac
    14821 taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta cagaactgta
    14881 gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt
    14941 tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat
    15001 atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca
    15061 ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata
    15121 aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc
    15181 agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt
    15241 aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga
    15301 taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc
    15361 tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga
    15421 aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg
    15481 ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa cagctaaata
    15541 tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta ataaaataat
    15601 tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa
    15661 gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat
    15721 aaccgtactt gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct
    15781 atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat
    15841 gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata
    15901 gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa
    15961 attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc
    16021 taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt
    16081 ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac
    16141 gtcatacact tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac
    16201 tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt
    16261 tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc
    16321 gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt
    16381 aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc
    16441 cattcaatta gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac
    16501 ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc
    16561 aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag
    16621 ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc
    16681 ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc
    16741 tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt
    16801 tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga
    16861 ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc
    16921 ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag
    16981 atacactggt tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt
    17041 ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt
    17101 tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt
    17161 gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata
    17221 acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt
    17281 tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg
    17341 aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt
    17401 gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg
    17461 tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc
    17521 taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag
    17581 aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt
    17641 atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat
    17701 tgtactgatt tgttgaggaa accttgcgtg ttggatcacc gtttctgttc caacaagaga
    17761 taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac
    17821 ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga
    17881 tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt
    17941 ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt
    18001 atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa
    18061 ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt
    18121 tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct
    18181 tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa
    18241 gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca
    18301 atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac
    18361 cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc
    18421 ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc
    18481 ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac
    18541 catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg
    18601 gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta
    18661 tgaataatgg taattcataa cggtctgtca attcattcat gacataacga aaccctagag
    18721 gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac
    18781 catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat
    18841 aataactaaa tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta
    18901 acattactgg tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc
    18961 ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata
    19021 cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac
    19081 ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa
    19141 acatatgatg acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt
    19201 tatctctagc agggaatgta accaatccag cagctgcata aggcatacga aaagcattat
    19261 tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct
    19321 gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta
    19381 ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat
    19441 ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt
    19501 ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact
    19561 ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa
    19621 gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa
    19681 tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt
    19741 cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt
    19801 ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa
    19861 gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa
    19921 attgaatgct actcttcatt tttaatattt atatataaca aatacataag acgatccccg
    19981 aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct
    20041 tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca
    20101 ataaccctta aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa
    20161 ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc
    20221 ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc
    20281 aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt
    20341 tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca
    20401 cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc
    20461 aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca
    20521 atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct
    20581 tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt
    20641 tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta
    20701 ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt ggataatact
    20761 tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga
    20821 tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa
    20881 taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg
    20941 tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt
    21001 gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca
    21061 atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt
    21121 ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt
    21181 tcatccaact gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca
    21241 ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc
    21301 tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg
    21361 atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct
    21421 gccatgattg cttgataatt actgatatca tacccattgt catcattcgg agactcgtat
    21481 actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata
    21541 atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt
    21601 tgataaacaa ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat
    21661 gtttctattt tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt
    21721 caaatagcta ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa
    21781 ttccctcaac tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac
    21841 ttctttataa acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat
    21901 ataagaaact attttttcgt ttttttctct aatactcagg ccagaacgct gatttcacaa
    21961 cctctttatt tcacattcaa tatccacatg aatcccacaa atacaaagaa cagaccatac
    22021 atgattggag atacttcttt tcctctttta gctgctaaca tggtaattgg atagaagata
    22081 aaacctaatg caatcccatc tgaaatacta taagtcaaag gcatgccgat caagatcaag
    22141 aatgaaggga tcgcaatctc taagtttccc cattcgattt gtcgcaaaga ttgtgccatc
    22201 aatactccta ccataatcaa tgctggtgca gttacttgtg aagtaacaac tgatagtaat
    22261 ggagaaaaga acaagcctaa caagaagaaa atccctgtag taatagctgt gatacctgaa
    22321 cgtcctccaa cggcgatacc agctgatgat tctacaaatg caccaacagg agaagtacca
    22381 aataaggaac ccgcaagcat agctgttgaa tcagaagcta aagctttccc tacacgaggc
    22441 atcttgttgt ctttcatgaa acctgcttgg tttgctaatc caactaaagt tcctgctgta
    22501 tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc acatttgaag agaattgatg
    22561 tcccctacat gttttaatgc aactaaaaat gttggtttca aacttggtgc tgcggcaaca
    22621 atttcagaag gcattggaat caaacctgtg accagaccta aaatcgttgt agctgccatc
    22681 ccgatgaaga tgccacctgg tacacgccga acaagcaaga tagcagttac gactaatcca
    22741 aaaatcgtca accaagtaga accaacgttg agtgatccta atcctactaa tgtagattca
    22801 ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag cgataaatag accgataccg
    22861 ccagaaatcg catatttcaa atctgctggg atcgcatcaa tgatcatttc ccgcagcttg
    22921 aagattgtga tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc
    22981 catggaatcc ccattcctaa acaaacagaa taagcaaaaa aagcattgat cccaagagca
    23041 ggtgccgttc caattggata acgggctaat acccccatta aaacacagcc gatcgcactg
    23101 gctaatgcag tcgcagtaaa cactgctcct tcatccattc cagaagcacc taatacagtt
    23161 ggatttacaa ataagatata agccattgag ataaatgttg taaatcctgc cagtacctca
    23221 cgttttaccg tggtgttcaa tttttctatt tcgaagtagg aaagtatttt ttccttcatc
    23281 ttccatcccc ctaaatccga acattataaa taaagtccgt taaaatttcg atttattaat
    23341 aaaacaacgt gttttatcat atactaaaac aaaacataag taaacaaaaa attcggattt
    23401 taagcaaaat ccgaacgttc catctgatta aatcccttta tctcaaagtt catgaacatt
    23461 cactttggat tacaagaatt tctttattgt gttaataaag tttcttcatt ctagtctcaa
    23521 aaatcaatag tgtgatttga ttttaccatt tcgtttctgt tcttctttga tgaattcttt
    23581 tgtatcgaca ggattcagat tatttagtcc gccaaaacct gtatcgacca tttttcccca
    23641 gccttttgtc ttcttttttc ctgatttgag attctttgct cgaagcttct cactatgttt
    23701 tgctcgccga tacttcaaat aaccttctaa tcttcccatt agatccactg tttttcctcc
    23761 tttttattca atcagacgac tcagcttttc ctataacaaa ataattgcct tcagggtcag
    23821 aaaaattgaa agtcaagatg ccattgttat ctgtgatttc tcctggttct tccatgttct
    23881 cgtagatagt ttcaaattca tccgtaaaaa atactaaaga aggcggtggt cctaatacct
    23941 ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc taattctaat tcttttgaga
    24001 tgctgagtac aatactctta aattcttctg gcaattcgat tgtttcagaa atttctgcac
    24061 caattttttc tacaaagaat cggcaaatca tctctacgtc attgacataa agcatcacac
    24121 gtgttttttg tatcatcatt tgacccttct ttctatttga ttttctttcc tgtctataaa
    24181 ctgattccat tatatcacga aagcgcaaac accttgtgtt tgatactatt caagaaaaat
    24241 aaagcaatag tttctaggat tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc
    24301 aatgaatgcc gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg
    24361 ttagtgtgtg tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt
    24421 cgattgcctg tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg
    24481 ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc acgccagttg agacagcgac
    24541 agatcgctcc ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat
    24601 ctgcacctaa aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg
    24661 cgatcttttc gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat
    24721 agatatctac aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta
    24781 aagcttcaat agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc
    24841 actgagcaat gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt
    24901 ttgctgcctc attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat
    24961 cgataccatt tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg
    25021 tatccactat tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa
    25081 tagatagtct gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa
    25141 tcctttttta cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat
    25201 ggctgttttt tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat
    25261 accttgatgt ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc
    25321 catttcctca gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc
    25381 gatactcact aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag
    25441 tgcataagct tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt
    25501 atctgtaaat gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt
    25561 tcgaatttcg tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt
    25621 gacaaaattc caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg
    25681 ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg
    25741 aatgatgaaa ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt
    25801 attttcttta tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg
    25861 ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg
    25921 attttcgcaa atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa
    25981 ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc
    26041 agccatttca tcccaccttt attttgtttg taagactagt tcacctcttt ttgttgcaaa
    26101 tgcaactatt ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt
    26161 ttcttctttt acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt
    26221 aactgtttct tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat
    26281 cccatgttgg aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa
    26341 gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca
    26401 aaaactgctc gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta
    26461 cgcacatttt cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct
    26521 ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca
    26581 tctacttttg gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa
    26641 gtttcttttc ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc
    26701 tctttatcaa atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt
    26761 ttgtattgag ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta
    26821 tgtaatcaag tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat
    26881 aaataaaaga atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga
    26941 ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga
    27001 cgagctattc ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc
    27061 cccactaaac ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca
    27121 tgagaagctg tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga
    27181 cctctcggta aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact
    27241 tattgtcatt gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat
    27301 agtctgttac atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg
    27361 cgatgatctt tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca
    27421 atacgtcacc atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat
    27481 cacgtttgta ccgacgttgc cataacactt caaagtctgc tgttaggcga atcgtgatca
    27541 cctgatatcc gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag
    27601 gatactcgga cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat
    27661 agaaaggata aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga
    27721 tggattctgc atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc
    27781 gttcccgtag tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa
    27841 tgaaatactt tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac
    27901 agtccaatct tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg
    27961 cgaagagact tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg
    28021 actaaaagct ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa
    28081 tcttcttctt tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg
    28141 atttctttca gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta
    28201 ccgatagcaa tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc
    28261 ggtcttgtat ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt
    28321 gttccaagtt ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt
    28381 gtcttcagga tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat
    28441 ccgctgtcga ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact
    28501 gcatcaacca tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg
    28561 atatatttag cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct
    28621 gcgcctagca tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa
    28681 ttcaactctt ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac
    28741 cttatctttt tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc
    28801 cattttgccc atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg
    28861 cttctggtta tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc
    28921 caaactcttt tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag
    28981 tccaacttgt tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg
    29041 tttcaattgt gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc
    29101 ttcttttgcc agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat
    29161 tttccctact ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag
    29221 tgctgcggct actgtatcta catcggttgc tgtagcattg ttgaatccca tcgaattaaa
    29281 gatcgttact agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat
    29341 gatcgctcct cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa
    29401 gaaattggtt agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat
    29461 ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata
    29521 aggaaagaaa actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat
    29581 ccctcgaaat gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata
    29641 cacacccact acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc
    29701 tgataattca gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac
    29761 catcacgacc atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt
    29821 gatatcttcg atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc
    29881 ttcaaataag tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat
    29941 tttacgaatg atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa
    30001 accaccgacc aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt
    30061 catcgttgcc atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa
    30121 acgagccaaa ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc
    30181 taaacccaaa tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg
    30241 catatgaaat ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct
    30301 cgaaccggca ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg
    30361 tcctgctgat ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga
    30421 tgcctgagtc aaaatatttt cgatgataaa attgataacg cccat
//
LOCUS       DACEMS010000021        30367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEMS010000021 DACEMS010000000
VERSION     DACEMS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.021"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            376..1065
                     /locus_tag="IW735_000857"
     CDS             376..1065
                     /locus_tag="IW735_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1043874.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1052..2260
                     /locus_tag="IW735_000858"
     CDS             1052..2260
                     /locus_tag="IW735_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1043875.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2554..3825
                     /gene="serS"
                     /locus_tag="IW735_000859"
     CDS             2554..3825
                     /gene="serS"
                     /locus_tag="IW735_000859"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1043876.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4070..4765)
                     /locus_tag="IW735_000860"
     CDS             complement(4070..4765)
                     /locus_tag="IW735_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1043877.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(4855..5955)
                     /gene="ychF"
                     /locus_tag="IW735_000861"
     CDS             complement(4855..5955)
                     /gene="ychF"
                     /locus_tag="IW735_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1043878.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(5964..6155)
                     /locus_tag="IW735_000862"
     CDS             complement(5964..6155)
                     /locus_tag="IW735_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1043879.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(6176..7066)
                     /locus_tag="IW735_000863"
     CDS             complement(6176..7066)
                     /locus_tag="IW735_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1043880.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(7053..7820)
                     /locus_tag="IW735_000864"
     CDS             complement(7053..7820)
                     /locus_tag="IW735_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1043881.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(7832..8545)
                     /gene="rsmG"
                     /locus_tag="IW735_000865"
     CDS             complement(7832..8545)
                     /gene="rsmG"
                     /locus_tag="IW735_000865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1043882.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(8777..10678)
                     /gene="mnmG"
                     /locus_tag="IW735_000866"
     CDS             complement(8777..10678)
                     /gene="mnmG"
                     /locus_tag="IW735_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1043883.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(10695..12092)
                     /gene="mnmE"
                     /locus_tag="IW735_000867"
     CDS             complement(10695..12092)
                     /gene="mnmE"
                     /locus_tag="IW735_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1043884.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            12436..12774
                     /locus_tag="IW735_000868"
     CDS             12436..12774
                     /locus_tag="IW735_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043885.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(12942..13700)
                     /locus_tag="IW735_000869"
     CDS             complement(12942..13700)
                     /locus_tag="IW735_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1043886.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(13718..14536)
                     /locus_tag="IW735_000870"
     CDS             complement(13718..14536)
                     /locus_tag="IW735_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1043887.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(14533..14889)
                     /gene="rnpA"
                     /locus_tag="IW735_000871"
     CDS             complement(14533..14889)
                     /gene="rnpA"
                     /locus_tag="IW735_000871"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1043888.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(15007..15141)
                     /gene="rpmH"
                     /locus_tag="IW735_000872"
     CDS             complement(15007..15141)
                     /gene="rpmH"
                     /locus_tag="IW735_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1043889.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            15664..16998
                     /gene="dnaA"
                     /locus_tag="IW735_000873"
     CDS             15664..16998
                     /gene="dnaA"
                     /locus_tag="IW735_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1043890.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            17196..18326
                     /gene="dnaN"
                     /locus_tag="IW735_000874"
     CDS             17196..18326
                     /gene="dnaN"
                     /locus_tag="IW735_000874"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1043891.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            18555..18797
                     /gene="yaaA"
                     /locus_tag="IW735_000875"
     CDS             18555..18797
                     /gene="yaaA"
                     /locus_tag="IW735_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1043892.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            18784..19908
                     /gene="recF"
                     /locus_tag="IW735_000876"
     CDS             18784..19908
                     /gene="recF"
                     /locus_tag="IW735_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1043893.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            19905..21851
                     /gene="gyrB"
                     /locus_tag="IW735_000877"
     CDS             19905..21851
                     /gene="gyrB"
                     /locus_tag="IW735_000877"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1043894.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            21871..24342
                     /gene="gyrA"
                     /locus_tag="IW735_000878"
     CDS             21871..24342
                     /gene="gyrA"
                     /locus_tag="IW735_000878"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1043895.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            24578..24877
                     /locus_tag="IW735_000879"
     CDS             24578..24877
                     /locus_tag="IW735_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1043896.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            24927..25439
                     /gene="ssb"
                     /locus_tag="IW735_000880"
     CDS             24927..25439
                     /gene="ssb"
                     /locus_tag="IW735_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1043897.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            25465..25701
                     /gene="rpsR"
                     /locus_tag="IW735_000881"
     CDS             25465..25701
                     /gene="rpsR"
                     /locus_tag="IW735_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1043898.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            25844..27817
                     /locus_tag="IW735_000882"
     CDS             25844..27817
                     /locus_tag="IW735_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1043899.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            27823..28275
                     /gene="rplI"
                     /locus_tag="IW735_000883"
     CDS             27823..28275
                     /gene="rplI"
                     /locus_tag="IW735_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1043900.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            28549..29916
                     /gene="dnaB"
                     /locus_tag="IW735_000884"
     CDS             28549..29916
                     /gene="dnaB"
                     /locus_tag="IW735_000884"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1043901.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         9315 a         5898 c         6025 g         9129 t
ORIGIN      
        1 cgactcagtt atcttatcat gtcatttcca acatgtcaac aacttatttt ttattattta
       61 ataagttatt ggtgttcttt tgaacaaata ataatatatc aacatcacat gtgaaagtca
      121 ataagaaaat gcttatttat caacatttct agcttacatg atcattggtt agttctggtc
      181 ctttttcgtt cgtctttata actccgagag acgtaaaatc cttttgtttt cagtttaatg
      241 ttcatgttct tgtcttattc acaccaacta gatgtctcac cttctaaaat tttccttaac
      301 aaagtgctgc ttttatctat ttctgctttt ttttgctaga ataatagtaa caatatgact
      361 ctggaggatt tatttatgaa aattttgatt gctgacgatg ataaagaaat cgttgaatta
      421 ttaagtatct atgttcacaa tgaggggtat gaagcagtaa aggcttatga tgggaaagaa
      481 gcattatcta aaatcagaac aatccctgat attgaattac ttattttaga tatcatgatg
      541 cctgagatgg atgggatgca agtagtgaaa gagctacgaa aagaatccca aataccaatc
      601 attatgctga cagcaaaaac tactgatatg gataagatca aaggactggt cgctggtgca
      661 gatgattatg tgaccaagcc atttaatccg ttagaagtaa tggctagagt aaagtcttta
      721 ttacggcgta caaaaatgca gattgcccca gatcagcctg atgtattaga agtaaatgca
      781 ttaatcatca ataaagactc tcatgaagtt aaaacaatcg atgggaaaga aatccagtta
      841 acagctttgg aattcggtat cctctacttg cttgctagtc atcctaatcg agtatttagc
      901 gcggatgaaa tttttgagcg tgtttggaaa caagaaagta ttgtctctgc taaaactgtc
      961 atggttcacg ttagtcattt aagagataag attgaagaag caactggtgg tgaaaaagtc
     1021 attcaaaccg tttggggagt gggctataaa attgacgcaa gatagtaaac gagcaactga
     1081 aaaacgccgt cggatcaatt tgacttcaaa agaaatcagc gaattgttag cggaaggaat
     1141 catcacgatt atccttttgc ttctattaaa tgtttctatt ctggttgtta tcagttcggt
     1201 gatcaacagt tcaccttctt tgacgaatgc gatttgggat tcaaagaata tttttgcaga
     1261 acgattgaac actgatttgt tctggaacgg ccggaatttt attattccct ttttctttct
     1321 attagatatt ggtgttttgt attggcgctt gattcgaaga tatcgccaaa tgcaattgcg
     1381 gcatatcatc agcgaacttc actacatcgc aaacggaaat tatgatcatc gtatcccttt
     1441 tgaactgagt ggtgacttaa gccgagtggt aacaagtatc aatggattgg tggatagtac
     1501 cgtagcagcg attgaagacg aacgtaaaat cgaaaaatca aaagatgaat tgatcacgaa
     1561 tgtcagtcat gatatccgta ccccactcac gtccatcatt ggatacctag gactgataga
     1621 agatggccag tatcactcag aagaagatct tttaaagtat acgcatactg cttatatcaa
     1681 agcaaagcag atgaaatctt tagtggacga cttatttgaa tacactaagg tacgccagcc
     1741 tgctgtcccg gtgaattttt ctgcctttga catgatccag ttgatcgaac agttagcagc
     1801 tgattttgag ttggaagctt ctaaaaaaaa tatccaaata cttgttcaat caaaagtaga
     1861 ctcccttatc atggatggcg ataccgaaaa acttgtacgt gtcttcaata atcttttgac
     1921 gaatgcgttg aaatacggaa aaggagcaac gaagatcgta atcgaagtag aacgtatcgg
     1981 ttcagaagtt gttgcaacag tcaaaaataa cggagcaatg atcccacaac aggcaatcga
     2041 taatctattc gaccgttttt atcgtgtaga agaatctcgt tcacaagcaa ctggaggaac
     2101 tggacttggt ttagcaatcg ctcaaagcat tgtggctttg cacggcggat acatttatgc
     2161 aaaatcagat aagcaatgga cttctttcat catgcacctt ccaatcaaga aaaatgaaaa
     2221 attacctatt gatacacagg aagttattga cgaaagctga aatattcgct attcttatta
     2281 caattattag aataaatgaa aaacacagaa cccagtacac tttattcttt ttcagagagc
     2341 tgatggcagg tgtaaatcag caaaggataa tttggaagtt ccagttcgtt tacgtgccct
     2401 ttcgaaataa gaaaggtcgg ttcaacagcc gttatgtgtt caacaagtgc agctgacgct
     2461 gaacttgggt ggtaccgcga atttcagact gattcgtccc aagaataatg ggatggatca
     2521 gtcttttttt attttcaaaa aggaggaaat catatgttag atgtaaaaat gatgcgacaa
     2581 aatattgaag aagtacaaga aaaattgatg actcggggcg taaaaaaaga aacattagct
     2641 gaattcatgg aactagatga aaatcgtcgt aagctactag tcaaaagtga agaattaaaa
     2701 aaatatcgaa atgatgtttc tgctgagatc gcccaattga agcgtgcaaa agaagatgct
     2761 tctgctaaaa tcgctgaaat gaaagaagtc ggtggcaaca tcaaagcatt ggatactgaa
     2821 attgctgaag tggatgaaaa attgaaacaa attgcaacta ctttgccaaa ccttccccat
     2881 gattctgtgc cagttggaac agatgaagag gacaatgtag aaatccgtcg ttggagcgaa
     2941 ccacgtacat ttgcttttga accaaaacca cactgggaaa ttgcagaaaa tcttgatatt
     3001 ttagattttg aacgtggcgc aaaagtagcc ggtagccgtt ttgtctatta caaaggatta
     3061 ggtgctcgat tagaacgtgc tgtctacaac ttcatgcttg atcagcatat ttacgaacac
     3121 ggctatactg aaatgatcac tccttatatc gtcaacagta aagcgatgtt tggtacggga
     3181 caatttccaa aattcaaaga agatgttttc caattggaga atactgatct aacattaatc
     3241 ccaacagctg aagtaccatt aactaactat tataatgatg aaattttaga tggtgcagat
     3301 ttacctattt atttcactgc actaagccct gcttttcgtt ctgaagccgg aagtgctggt
     3361 cgggatacac gaggactgat tcgtcttcac caattcaata aagtagaaat ggtcaaattt
     3421 tcggatgtag agcattctta cgaagaattg gaaaaaatga cagctgatgc agaagatatc
     3481 ttgaaaaaat tgcatttgcc ttatcgtgtg atggccttat caactggaga catgggcttt
     3541 tctgcagcta aaacttatga cttagaagta tggatacctg cacaagatac ttatcgtgaa
     3601 atcagttctt gttctaactg tgaagatttc caagcacgcc gcgcaatgat ccgttatcgc
     3661 gataatgatg gaaaaatcca ctatactcat actctgaatg gttcaggact tgcagttggc
     3721 cgtacagtag ctgccatttt ggaaaactac caaaatgaag atggttctgt cacagttcca
     3781 gaagcgctcg ttccttatat ggggaatctg aaagttatta aataaagagg ctgtgaaaaa
     3841 gccgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
     3901 aaattggact taataccgag aaactggctt ttgaacaccg tttatcgtaa aaagggactg
     3961 tgacaagact tttgtcacag tcccttacta ttatttaact atggataaac aaaatgaggt
     4021 tctttactac ccctagatat ctagcaaaat agctgatctt tttgctagtt tatcttcttg
     4081 ggacggataa acttccaata atcccgtatt ttttcttcaa gtagtaacgg attgctaaag
     4141 cagttgcccc gataatgata actactactg gatctaatac tggattgata actggtgtca
     4201 atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt cgttccacct aggataagca
     4261 atgtcttacc cattcctggt ttcttgcttc gatctgctcc gggatattca tattgataga
     4321 tgtatttgta catcatataa aagacccagc caccgattgc agaacctaga acgagtgtag
     4381 aaaagccata agctggcgtt gttccacgtg aaaacaatcc ggtaatcgca atcatgatac
     4441 ttaatagacc aagcaacagc aacgtattat ctaaccacat caataaaggg gtggatgaat
     4501 tttttacttc tgctggctta ttgatgattg cttccgttct ttcagagact gtcccaaaca
     4561 attgacgggc tgttcttccg cctttttgtt ctgcaactaa agtaggcaga atgtcatgaa
     4621 gggcttttac tttttcttct tcagacaaat tcgctgcttc taaagatttt ttcaaatcaa
     4681 aaatgtactg ttgatttctt ttggttaatt ttgtttctaa actattgttt tcttttacga
     4741 ttgctcgtaa ttgttctggt tccattatct aatagtcctc cttagccgtt ccttttctca
     4801 acggtcgtca caaaagacat tctaccatat tctacagcta gtttgatagc ccttttacac
     4861 attaaagcgg aataacatta cgtcgccgtc ttgtacgaca taatcttttc cttctaatcg
     4921 aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa tgatccaagt cttcaaaaga
     4981 gacagtttct gcacggataa atccgcgttc gaaatctgaa tggatgattc ctgctgcttg
     5041 tggtgctttg attccttttt tgaatgtcca agcacgtact tcttgttccc cagcagtaaa
     5101 gtaagtagct aagcctaaca aatcataagc agcacggatc aactgatcca aacctgattc
     5161 ttcgattcct aaagcttcta aaaattccgc tttgtcttct tcatctaatt ctgcgatttc
     5221 ttcttctgca cgagcactga ccacgatcac ttctgcattt tcttctgcag caaagttgcg
     5281 cacttcttgt acataatgat tcgcgtctgc atctgctacg tcatcttctg agacattggc
     5341 tacataaaga attggttttg tcgtcaataa aaatagtgat ttaacgattt tttgttcttc
     5401 ttcagtaaat tcgatcgttc gggcagattt tccttcttcc agtacaggtt tgattttatc
     5461 cagtacagct agttctgcca ctgcatcttt atctttggtt ttagcaactt ttgctacacg
     5521 agtatagcgt ttgttgatag attctaagtc tgctaacact agttccaagt tgatcgtatc
     5581 aatatcttct agtggatcaa cacgtccttc tacgtgagta atattatcat catcaaaaca
     5641 gcgaacaacg tgacaaatag cgtctacttg gcggatatgg cttaagaatt gatttcccaa
     5701 accttctcct ttacttgcgc cttttacgat tccagcaata tctgtaaatt caaaagtcgt
     5761 cggaacagtt tttttcggat gtacaagttc tgttaagcgt tgcaaacgcc agtcaggtac
     5821 ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga tagtttgctg cttctgctcc
     5881 tgcttttgtg atcgcattaa acaaagttga ttttcctacg ttgggcaacc caacaattcc
     5941 agcagtcaat gccataaatt tattcactct ttctctgcat ttttttcaat tacttttttt
     6001 aatttctttt caaaatctcg acgggtcatc atgacgatat gtccgcaatt catacatctg
     6061 attttgatat ctgcgcccat tcgaatgact tcccagcgat tcgtctggca agcgtgaggt
     6121 tttttcattt caacgatatc gtgcaagtcg tacataaaaa tagcctcctt tttgtttatt
     6181 cttcatcaaa ttgaatattc aagatatcaa gaattcgagt caaatctttt tgagaaagat
     6241 attcaatctc gatcttacct ttcccatttt tttcttggat agctacactt gtaccaaatt
     6301 tatccatcaa acgatcttcg ctttctcgga tataatatgg tttttccttt gctagccttg
     6361 ggattttttt cttttctttt ccatcatttt gatttaactc agaaacaagc tgttccagtt
     6421 ggcggacagt taaattttca gttactgcac gattggctaa tttcaagatc aatgttttat
     6481 tttttaaacc taacagcgta cgggcttgtc ccatagataa acgctgatct tgtaccattt
     6541 ccttgacaag atccggtaaa gaaagcaagc gcaaataatt ggcgatataa ggtctgctct
     6601 ttcctaaacg actagctact tctgcttgtg ttagtttcag gtttttcatc agcatctcat
     6661 atgcttctgc ttcttctaac ggattcagat cttctcgctg caagttttcc agcactgcta
     6721 cttgcatcat cgcttcttcg tcaaagtctc taacgatagc agggatcgtt tctttttcag
     6781 ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat ctcatatcct tttactgcgg
     6841 aacggcgtac gatgatgggt tgaaagacac ctgattgttc aatagaatta gctagttcct
     6901 gcaaagatac ttcttcaaat gttttacggg gctgatacgg atttggtcgc agctcattca
     6961 atggtagctg gacaacggta tcatctttta catccaccgt ctctaagttt tctaaatctt
     7021 gaaacagtgc atcgattcct cggcctaatc ctttactttt cttcacgact taccacttcc
     7081 tttgccaatg cttggtagac ttcagctcct cgagaacgtg gatcgtagtc gatgatcggt
     7141 tttccatgac taggtgcttc tgataaacga atatttcttg gaatgatcgt ttcgtaaact
     7201 ttttcgcgga agtatttacg tacttcttct acgacttcat ttcccagatt tgttcgtgca
     7261 tcatacattg tcagtaagac tccctcgatc tctaattctg gattaaagtg tttttggact
     7321 aaacgaaccg tattcagtaa ttggcttaat ccttccaatg cgtaatactc acactgaaca
     7381 ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg tcagatggcc aagagaaggc
     7441 gggcaatcga tcaaaatata atcatactga ctgcttactt ctgctaaaga tccttttaat
     7501 cttgattctc ttgccatcat tgacgtcaat tcgatctctg ctcctgctaa ttggagagtc
     7561 gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag ttgcctcatc aattggcaat
     7621 tcattgacta gaacatcata gatgtctctt gtcacatcag gttttctgat cccgacacca
     7681 ctggtagcat ttccttgtgc atccatatcc actagcaata cttttttgcc aaggctagca
     7741 agacaagcac ctagattcac cgtcgtcgtt gttttgccga cgccaccttt ttggttcgca
     7801 acagatatta tttgtgccat ctcttctcct cctatttgat tggctgctta ttaggcagtc
     7861 cgggctttct tgggtatttc tttggggttg gtttcttttt ttgaataacg atgatatgcc
     7921 gttgatcaga gacattcggc aaggtaaccg cctcttcttt gaggaattta ccacctaata
     7981 aagcaatcgc ttgttttgct tctgtcaatt cctcttcact ttttgctgct ttcaatgcat
     8041 aaaaatagcc ttcttgttta gccaagggca aacacaattc cgctaacaca ttcaaacgag
     8101 caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa gtgaggattt tgcccaaatg
     8161 tttctgcccg gtcatgatat aaggaagcat cgattcctaa taatcgggtt aactcagata
     8221 agaaggttat tcgtttattc agagaatcaa cgatcgttat ctgcagttct ggaaaggcaa
     8281 tttttaaagg tatgctagga aaccctgccc ccgaaccaac atcgcataaa gataaaggtt
     8341 gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta aaaatgcttc agatatacgt
     8401 ctttttcatc cgtaatagct gtaaggttca tcttttcgtt ccattcgact aatgtccgat
     8461 aataaatagc gaattgattc atttgatgat cggtaagctc tatccctttt tctgccaagg
     8521 attggcgaaa ttcttctggt atcataaata ttcctttcat tgtgaaacag caattgtttc
     8581 acagattctc tccctacttt aacgcaaaat ctctagacat gcaatccttc catatggatt
     8641 cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt ctttctagta tgactcataa
     8701 aatatacaca gaagagagaa ctagctttga gcaatttctg caaaaactag ttctctcttc
     8761 tatttattat tacgtattac gctggttcac tgactttcgc aatcttccct tgttcgatat
     8821 agaccattag gatacttagg tctgcagggt ttacgccact gattcggcta gcttgggcaa
     8881 ttgtttctgg ttggattttc tgcaattttt gttttgcttc tgttgctaat ccgttgattg
     8941 ctgcgtagtc gatgttttct ggaatctttt tcgcttccat gcgttttaat ttttctactt
     9001 tttccatggc ttttttgatg tagccttcat atttgatttg gacttctact tgttcgattt
     9061 cttttcgtgt caattcttca ttttctggaa taaaccgtag aagttctgca taagtaatct
     9121 caggacgtct taaaaagtcg cttgctagta caccatcttt taatggtgcc gcattaatcg
     9181 ttgctaaaaa gtcttggact tcttctgttg gtttgattcg tacattcatc aaacgtttga
     9241 tttcttgttc aactgcttct tttttcttca aataattctt gtattgatct tcttttacta
     9301 agcctacacg gtgacccatt tctgttaatc gcaaatctgc attatcatga cgtaaaagca
     9361 gacggtattc agcacgtgaa gtcagcaaac gataaggttc gttcgttcct tttgttacca
     9421 aatcatcgat catgacaccg atgtaaccgt cacttcgttt gatcactaaa ggttcttttc
     9481 cctgaacttt caaagctgca ttgataccgg caatcagccc ttgtccagcc gcttcttcat
     9541 aaccgcttgt accatttgtt tgtccagctg tataaagatt ttcaacgact ttagtttcta
     9601 atgtcggacg gagctggtgt ggctctacta cgtcatattc gatagcatat cctgtacgca
     9661 tcatttctac tttttctaat ccagcaatag aatgaagcat gttgacttga acatcctctg
     9721 gtaaagaagt agataaacct tgaacataga cttcttccgt atttaagcct tctggttcca
     9781 aaaataattg atgacgtggt ttgtctgcaa aacgcacgat cttatcttcg atggatggac
     9841 aatagcgagc gcctacccct tcaacgatcc ctgtaaacat tggggcacga tgtaagttgt
     9901 ctcgaataat ttggtgagtc gtctcacctg tataggtcag ccagcaagat tcctgatcta
     9961 gtttataggc actatctggt gtgctgaagc taaaatgatt cggctctttg tcaccaggct
    10021 gctcttctgt aacagaatag tcgatcgtac ttgattttac tcgaggcggt gttcctgttt
    10081 tgaaccgttc gatagcaaag ccaagttctt ttagatgatt cgctagtcca atagaaggtt
    10141 gagagttatt cggacctgat gaatatttca attcaccgat aatgatttct ccgcgaagtg
    10201 ctgttccggc tgtaataaca actgctttgg cccggtagct cgctcctgtg gacgtcacta
    10261 ctccatgaca aacaccatct tcgacgatca gttcttcgac gattccttgt cttagcgtca
    10321 aatttggttc tttttcgatc gtatgtttca tttctgttga ataagcatgt ttgtctgctt
    10381 gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt caacatacgc atttggatat
    10441 aggttttatc gatattctta cccatttctc cgcctaacgc atcgatttct cgaacgacaa
    10501 cgcctttagc tggtccgcct acagaaggat tacatggcat gaatgctacc atgtccaaat
    10561 ttattgtcag taataatgtt tttgatccca tacgtgcagc agcaagtgca gcttctgaac
    10621 cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc tgcttcataa tgttccaatc
    10681 gtttgttcct ccttttattt tcctaaacaa aattgactga ataattgagt gattagctca
    10741 tcttgtacac tgtcgccgac aacttctcct agatagtccc agcaacgcgt catatctatt
    10801 tgaacgagat ccacaggcat tcctgaatca atacctgcaa tgacttcttc aagtgagagg
    10861 gaagcttttt ctaacaacgc gatatggcgg gtattagaaa cataagtagc gtctctttct
    10921 cctgtttctc cgctgaagaa taaatcagaa atagctgatt ccaaacgatc tagtccgtcg
    10981 tttttagcta cggaaattga aaaaactggt tcattttcga tcaatttctt caatttttct
    11041 tgttccagct gtgctggcaa atctgtttta tttagaagaa tgatccgttt caatccactt
    11101 gtcgcttcca ataattgttc atcttctgct gtcagtggtt cgctttggtt tagaacaagt
    11161 aagatcaagt ctgcttctgc taatgctttt cggcttcttt cgaccccgat ccgttccacc
    11221 acatcttctg tttcccgaat tccagctgta tcgattagct tgagcggtac cccccgaaca
    11281 ttgacatact cttcgattac atctcgagtc gttcctgcaa tatccgtaac gattgctttt
    11341 tcttctctta acaaatggtt taacaaactt gatttgccga catttggccg gccgatgatt
    11401 gccgtactta acccttcccg cagaaccttc ccttgcttgg atgtagcaag tagcgcttgg
    11461 attctttgtt ggatcatctg cgctttttca agcaataact tcgtagtcaa ttcttcgaca
    11521 tcatcatatt ctggataatc aatatttact tctacttgtg ctaatgtctc taaaatctct
    11581 tgtctcaatg agcggatcaa agcagacaga ttgccgtcta actgattaag agctagtccc
    11641 attgctttgt ctgtttttgc ccgaatcaaa tccatcacag cttctgcctg tgaaaggtca
    11701 acacgaccat tcaagaatgc tcgttttgtg aattcgcctg gctcagctaa tcttgcacct
    11761 tctcttaaca atagctgcaa aatttgattg actacaacga tcccaccgtg acaattgatt
    11821 tctactacat cttcccgcgt aaatgtcttt ggtgctctca tcacggagac catgacttca
    11881 tcgacaagct gattgctctt gggatcaacg atatgtccat aatggatcgt atgacttggt
    11941 acttctgata atcgtttgtt tccagattga taaactttat ctgctagttg cacagcttga
    12001 tcgccactta aacgaacgat actgattgca ccttctccag gtggggtaga aattgccgca
    12061 atcgtatcaa attctaaagt aatttcagcc attttttcac ttcctttttc attttgcgca
    12121 caaaaaaagt gcccactcca ccctttatgc acaataaacg gtgaacttcg cactttgact
    12181 gcttgttctt gattaaattc tggtcataga ttatcacact tgctataagg aatcaagtgt
    12241 ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat taccttatat ttaattgtcc
    12301 ttggatgtct ctttgcgttc tttggtttat tcgaagagaa gatactgtat aaacaaggct
    12361 ttatctttct taaaaaaaac ggtacactaa atggtagcgc tatctaaact atacactttt
    12421 gaaaggagaa aatatgtgag cccgttcaga tctgctgcat tggaagaaga aagtgaccct
    12481 cggatcaacg atgtcgtttt atctacaggg aatatcaacc agaaatatat cgtccgcgat
    12541 atcgtttttg ttgtggaatg cttagaagct gatttgtttg acccatcttt tcccaaagat
    12601 gaccttttct taacaacgaa gaggaaatta aagacgaagg tacttgaata tggtgcgaat
    12661 gcagtaatca actgtcattt tgagtatcag caaaaaaaac gcgacggaaa gtattttctg
    12721 gaactttttg cttatggaac agtgatccaa tttatccaaa caacgattgg ttaaatgaaa
    12781 cagacaaaaa aagcggatca aaactgaaga cagtttcgat ccgctttttt ctatcaaagt
    12841 tagtgactac ttcatcgtat actctgataa gaatgatttc tagtaaaata tacttggttt
    12901 gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga atcaataata ttttttagca
    12961 ggttctacta ctaaataacg ataaggttcc tcaccttccg aatgtgtttt tacatgttca
    13021 tctttgctta atgcagcatg gatttgttta cgttcaaatg caggcattgg ttctaagaaa
    13081 acaggctgcc ctgtccgttt tactttttca gccgtgcgat cagctaaacg ctgaatgatc
    13141 tcttggcgct tttcacggta attgcctaca ttgactacta cagacagctt atttcttgct
    13201 aaacgatgga tgaagacttg tgccagatat tgcaatgcat tcagtgtttt tccatgtttc
    13261 ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa caaccatatt atttgattct
    13321 ctttccattt tgacaagagc tggagcatca agctgttttg aaatttcagt aagataaact
    13381 gccaattccg ttaaagctgc ttcatcacct agctcactta cttcttctgt tttttcttca
    13441 agaatcgttt cgttgactac ctcgatctca gctgttggtt cttcttccaa aatttcttct
    13501 acagtctgtt ctactgtttc agaaactttt tcgctgatac ttggctggat cgaaagccgt
    13561 gcattctttc gacccattcc taggaaacct ttttttcctt cttccagtat ttcgatttcg
    13621 acttggtctt tggccaaacc caaatcagcc agacctagtt ggacagcttc gtctattgtt
    13681 gcagcttcat aaatcggcat tagcattacc tcccctatta tttccgtttt ttcttaggat
    13741 tcattgcttt cttaagagca cgttctcttt cgcggattct ccgtgcttct tcttcacgct
    13801 cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg tacaacttgg aaggcattgg
    13861 aaactaccca gtataatgaa agaccgctgg ctaaattgac acccatcact aaaatcatta
    13921 caggcatcac gaaattcatg atcttcattg atgcattcga ttcaatttga ctcatgctag
    13981 ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat cggcaaaata aatgttggat
    14041 ccgcgttccc taagttcaac cataagaaat gcccttgttt caactctggt acacgtgaaa
    14101 tagattgcca taacgccatc aagattggca tttgtactaa taaaggaaga caaccagcat
    14161 atggattgac gccattttct gcatataaac gctgctgctc ttctcttaac ttactttgtg
    14221 tttccggatc tcttgaacta tattgttgct gcagtgcttt taatttcggt tgtaattctt
    14281 gcgttttacg catgctcttt gtttggaaat gcatcaacgg caataggatc acgcggatga
    14341 tcagtgtaaa taaaatgatc cctatccctg cattaccaaa tgacaacgcc ttgatcgcct
    14401 cagcaaagta ataaacaata taacgatccc aaattcctgt actctgtgcg ctgacttccc
    14461 cagttccgca accggataag acgatgacca aactaacgat accggccatc aaaagtaaac
    14521 gtttgtattt cttcacttac tcttttcctc tctgtcgatt atttttgcaa gttttaagac
    14581 gtggactaaa ttggagcgaa cctcatcata agtcaacccc ttcacacttg gtctagcaat
    14641 cacaataaaa tccaactccg gatcaatttc ttcttttata gaataaactg ctgctcggat
    14701 ttttcgtttg acttcattgc ggtttaccgc gttgccgacc ttttttccta cagataaacc
    14761 aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa acgacgaatt tgcggttagc
    14821 aaaagaatct cccgtatgga acacggcttg aaactctcgt tcttttttta ctcgatacgc
    14881 ttttctcatt agttttcttt ccttttctat ctctcactta cgctcataat cataccataa
    14941 ttacgattgt cctttcatca tttccctaaa aaagagtata aaaaaaacca ctgaaacccc
    15001 agtggcctaa gctgaaagaa cttttcttcc tttgcgacga cggctagcta acacacgacg
    15061 tccattttta gtactcatac gtttacggaa tccgtgaact ttttgacgtt tacgtttatt
    15121 tggttggtat gttcttttca tatattccac ctccaaaatg ttcttatcta ttttataaac
    15181 acagacatac tttgatagta taacgacaaa gtagtgattt tgtcaatata aaatagaaga
    15241 tcgattgtta aaacttatcc acaagattat ttttttctat gggaatccta tgtggaaaaa
    15301 gttatgcaca gggattatcc acagataata aaaggctggt tttacacaga aaacacacgt
    15361 gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat tccgtctcct aactattggt
    15421 atatctgtat tttttatcca cagacgcaag aggaaatcta ttggttttta cgagttttcc
    15481 acctgtgcat tagtggtgtg gaaaactttt tttgacaact gtgtttttct cttttttctt
    15541 tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa
    15601 ctattgaaaa aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct
    15661 tatatggtat ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta
    15721 tcgcctgcta gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac
    15781 cagctcgttg ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg
    15841 acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt
    15901 actggcgaag aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca
    15961 cctagtccgc taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt
    16021 ggtaaaggaa accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct
    16081 atttataacc cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat
    16141 gcaattggtc atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt
    16201 gaaacttttg ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt
    16261 caagaatatc gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa
    16321 gaagctacgc aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa
    16381 atcgtactaa ccagtgatcg gttgccaaac gagattccga aactgcaaga acgccttgtt
    16441 tctcgctttg catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca
    16501 gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc
    16561 tacatcgcag gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg
    16621 caagcttttg ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg
    16681 aaaacgttaa aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa
    16741 gtggcaaagt attatcacat ccaattgaaa gatctcaaag gaaagaaacg cgtcaaatct
    16801 attgttgttc ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta
    16861 ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa
    16921 aaaatccagc agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa
    16981 aactttttac taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga
    17041 aaaaaacgtt caaaagttat acacactttt tccacagctg ttttttcgtt tctctcttac
    17101 gttttttgag ttttccacag aatcaacatg ccctattact tttattattc tttttaacta
    17161 ataataaata aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta
    17221 gctttatgca ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta
    17281 ttttgacagg tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg
    17341 ctgatatatc aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg
    17401 aatctactgg ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc
    17461 ctgaagaaac atttacttta gaagttttag aaaataaaca agtagcgatc acttctggaa
    17521 aagcgaattt tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg
    17581 aaagccataa ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag
    17641 tttttgctgt ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat
    17701 ctgataattc tttattagct gtagctactg attctcaccg tctaagtcaa cgcgtgattc
    17761 cagtagaaca agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat
    17821 tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag
    17881 tgttgttcaa aacagaaacg atgtatttct attcccgttt gctagaagga aactatccag
    17941 ataccaatcg tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct
    18001 ttttagcggc gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc
    18061 gtttatctat tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag
    18121 tagaagaaag cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc
    18181 cggattatat gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta
    18241 tctctgctat tcgtccattt acattggaac caacggaaga tggcgttcaa tttattcagc
    18301 tgattactcc tgtacgtaca aattaacaat gattttcaat gttcttgaaa atttaattga
    18361 aaagaaatag aaaaaataaa attttaaaca gaaggcccta aaatctcttt taaggctttc
    18421 tgtttatttt tgtttaaatc acctttttta gattaaatcg tcttatatgg aaaaatatat
    18481 ggataaattt gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa
    18541 atgagggatg ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc
    18601 aaatgttgaa agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag
    18661 aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg
    18721 gcatgatggt tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg
    18781 ataatgaggc tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc
    18841 gaattttcca agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt
    18901 ttggaaagta tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa
    18961 ttgattggct ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc
    19021 actcttccat tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc
    19081 gaacagaaaa agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa
    19141 gatctttctt tagtaaaagg cagcccgcag atcaggcgga agtttttaga tatggaatta
    19201 ggccagatca atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa
    19261 agaaaccagt acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat
    19321 attttgtccg aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc
    19381 gtcaagcgat tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag
    19441 cagctggaaa tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa
    19501 atccaagaac aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga
    19561 acaacgacag ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg
    19621 cagacttatg gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa
    19681 atcgatttga ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg
    19741 agcgagctgg atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa
    19801 acgttcttaa caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag
    19861 attttttatg ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa
    19921 gtttagtaga acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg
    19981 aagccgttcg taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc
    20041 accttgtttg ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga
    20101 aaatccatgt gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga
    20161 tacctgtaga tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt
    20221 tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag
    20281 taggatcttc tgttgtaaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag
    20341 gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg
    20401 aacaaacgga tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag
    20461 agacaactga gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga
    20521 accgcgggat gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat
    20581 accattatga cggcgggatc aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga
    20641 tttttccaga acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta
    20701 tgcaatatac agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt
    20761 atgaaggtgg aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt
    20821 atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc
    20881 gagaaggatt gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa
    20941 caaaaacaaa attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac
    21001 atttcatgaa attcttgctg gaaaacccaa gtgtgggccg gcaaattgtt gaaaaaggat
    21061 tactagcttc cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag
    21121 gtgctttaga gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg
    21181 aaaaatgcga attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc
    21241 gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag
    21301 caagcatgga caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta
    21361 cagggtttgg agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga
    21421 ctgatgccga tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt
    21481 acatgcgtcc tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg
    21541 taaaacaagg gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga
    21601 aacaagtcgt tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac
    21661 ttggagaaat ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga
    21721 tggcccgcgt cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg
    21781 gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact
    21841 tagatattta atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat
    21901 gatgtcaatc taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt
    21961 gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta
    22021 tacggcatga acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc
    22081 gtcggagatg tcatgggtaa ataccatcct catggagacc gtgcgatcta tgagtcaatg
    22141 gtacggatgg cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt
    22201 ggttctgttg acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa
    22261 atcgctacag aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat
    22321 gatgatacag aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac
    22381 ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag
    22441 gtagtagcgg ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg
    22501 gaagtattac ctggaccaga ttttccaaca ggtggtttgg ttatggggaa atcaggaatc
    22561 cgaagagcgt atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact
    22621 gagatgccaa atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa
    22681 gcaaaattga tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca
    22741 gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac
    22801 gccagcgcat ccgttatctt gaataactta tataaattga catccttgca aaattcattt
    22861 ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatc
    22921 cttgaaaact atatcgaaca tcaacgcgaa gtcatcacac gacggacggt ctttgaaaaa
    22981 gaaaaagcag aagcaagagc acacatctta gaaggtttgc gtattgcttt ggatcatatc
    23041 gatgaaatca ttgcgattat ccgcgggtct aaatcagatg acgaggcaaa agcaacaatg
    23101 atcgaacggt tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc
    23161 cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg
    23221 attgaagatt taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat
    23281 gaattagcag acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt
    23341 gaggtcttaa gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg
    23401 acaaacaatg gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt
    23461 ggacgtggtg tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct
    23521 tgttctaccc atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa
    23581 ggatacgaaa ttccagaata cgggcgtaca gccaaaggaa tcccaatcat caatttacta
    23641 ggtatcgatt cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa
    23701 ggccactatc tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct
    23761 tttgctaaca tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc
    23821 gtcaatgtgc tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac
    23881 tctgtcactt tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggtcaga
    23941 gggatacgcc tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaaaatcaa
    24001 gaagtattag tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca
    24061 gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca
    24121 ctagctggat tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt
    24181 gtcattatcc gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta
    24241 cgtttgatga agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa
    24301 gctgcagaac aaacagttga agaacaagga tcagaagaat aaccatcaag actaagtgtg
    24361 taagctagca agaataaccg attaaaaaat ctggttattc ctgctggctt ttttgattag
    24421 ttgaatttct cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga
    24481 gtgtataatt agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga
    24541 acctaagagc cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat
    24601 catgtacatt attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt
    24661 cgatgcaatc ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg
    24721 ccgtttcgct tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc
    24781 ttctccatca tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga
    24841 tatcattcgt cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata
    24901 gaaaacgatg agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga
    24961 cgaaagatcc agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg
    25021 ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg
    25081 tgatttggcg caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag
    25141 gtgttactgg acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg
    25201 tgacagaagt agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc
    25261 gccaacaaag cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca
    25321 ataactctaa tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt
    25381 ctgacccatt cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa
    25441 cgaatttgat aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt
    25501 aaagtagact acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta
    25561 ctaaaaagat ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc
    25621 gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta
    25681 cctttcgtta gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc
    25741 tattcaattc ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga
    25801 aagttatggt aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa
    25861 tcatttatcc ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt
    25921 cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg
    25981 tgttcgctat tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca
    26041 gcaggcttcg actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg
    26101 agtgattgct tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa
    26161 aatcaaagat caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa
    26221 agaaaaaaag aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac
    26281 agtaaagggt gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca
    26341 gacgaacagt cagccggtga ttggtatttt atctattgat aactatgcag atgccattga
    26401 taaattagat gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga
    26461 ctggatgaat gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt
    26521 agcacagatg atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt
    26581 tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg
    26641 ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt
    26701 ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta
    26761 tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac
    26821 cgctttgcgt cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc
    26881 ggatatggat gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa
    26941 aaaagcttac atcgttatca atgagaacga gatcattcca gatgtagaac ggtgtcttga
    27001 agaaatccat aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga
    27061 gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat
    27121 ctctcagggc ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg
    27181 ggaagagttt ccaagtaaac ctctgctgac ttatattgaa tcttcagctt cttcagcttc
    27241 agagctagtt tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt
    27301 tgaggccaca ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac
    27361 aactgcacga acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt
    27421 agtacaatat ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc
    27481 tggaagcgac tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta
    27541 taatagtgtt atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc
    27601 agctttcgtc attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg
    27661 cacgatcaac gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc
    27721 agctactcaa ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat
    27781 caaagaaaat atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta
    27841 caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca
    27901 caaaattatc taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag
    27961 ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa
    28021 aagttaaaag aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg
    28081 gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa
    28141 caatacaaaa tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta
    28201 ggctacacaa acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat
    28261 gtcgtacaag aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga
    28321 aaaaagacaa accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca
    28381 aatcgatttc agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa
    28441 cgagtataat aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc
    28501 tctcattttc ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg
    28561 tggcaggatc gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt
    28621 tttcttgacg cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat
    28681 cgtcgtggcc atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg
    28741 attgacgtgg ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt
    28801 ggtttaagtt atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac
    28861 tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga
    28921 atcgttacga aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa
    28981 cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa
    29041 gtactgaact ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca
    29101 ggtctaccaa ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa
    29161 ctaatcattc ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct
    29221 caaaatgtag gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca
    29281 gaatctcttg taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt
    29341 acaggtcagc tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct
    29401 cgagcaaata tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa
    29461 tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag
    29521 ttgattgaag gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa
    29581 ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt
    29641 ggagtagaac agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca
    29701 attgaacaag atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa
    29761 ggcggagatg acgatgatgg aaacgagcca aaaaatgaca acatcatcga agtcatcatc
    29821 gagaagaacc gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat
    29881 aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca
    29941 atcccgagag ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta
    30001 atttaaacag atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa
    30061 taaacggtgt tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga
    30121 actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt
    30181 tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg
    30241 agaagcagtt ttttgaaatt gctccttgtg agtcagagtt aaacggcagg ctcacaacct
    30301 ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa
    30361 tatgaga
//
LOCUS       DACEMS010000022        29050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEMS010000022 DACEMS010000000
VERSION     DACEMS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.022"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(48..1112)
                     /locus_tag="IW735_000885"
     CDS             complement(48..1112)
                     /locus_tag="IW735_000885"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1043902.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1109..2194)
                     /locus_tag="IW735_000886"
     CDS             complement(1109..2194)
                     /locus_tag="IW735_000886"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1043903.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IW735_000887"
     CDS             complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IW735_000887"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1043904.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IW735_000888"
     CDS             complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IW735_000888"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1043905.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4443..5096
                     /locus_tag="IW735_000889"
     CDS             4443..5096
                     /locus_tag="IW735_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1043906.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            5181..6086
                     /locus_tag="IW735_000890"
     CDS             5181..6086
                     /locus_tag="IW735_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1043907.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(6088..6720)
                     /locus_tag="IW735_000891"
     CDS             complement(6088..6720)
                     /locus_tag="IW735_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1043908.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            7040..7564
                     /gene="infC"
                     /locus_tag="IW735_000892"
     CDS             7040..7564
                     /gene="infC"
                     /locus_tag="IW735_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1043909.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7636..7836
                     /gene="rpmI"
                     /locus_tag="IW735_000893"
     CDS             7636..7836
                     /gene="rpmI"
                     /locus_tag="IW735_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1043910.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7889..8248
                     /gene="rplT"
                     /locus_tag="IW735_000894"
     CDS             7889..8248
                     /gene="rplT"
                     /locus_tag="IW735_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1043911.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8600..9838
                     /locus_tag="IW735_000895"
     CDS             8600..9838
                     /locus_tag="IW735_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1043912.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            9886..12015
                     /locus_tag="IW735_000896"
     CDS             9886..12015
                     /locus_tag="IW735_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1043913.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11990..12679
                     /locus_tag="IW735_000897"
     CDS             11990..12679
                     /locus_tag="IW735_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043914.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(13087..13773)
                     /locus_tag="IW735_000898"
     CDS             complement(13087..13773)
                     /locus_tag="IW735_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1043915.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13909..14103)
                     /locus_tag="IW735_000899"
     CDS             complement(13909..14103)
                     /locus_tag="IW735_000899"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043916.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(14153..14593)
                     /locus_tag="IW735_000900"
     CDS             complement(14153..14593)
                     /locus_tag="IW735_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1043917.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(14597..15442)
                     /locus_tag="IW735_000901"
     CDS             complement(14597..15442)
                     /locus_tag="IW735_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1043918.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15591..17009
                     /gene="pepV"
                     /locus_tag="IW735_000902"
     CDS             15591..17009
                     /gene="pepV"
                     /locus_tag="IW735_000902"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1043919.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFININFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(17085..18287)
                     /locus_tag="IW735_000903"
     CDS             complement(17085..18287)
                     /locus_tag="IW735_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043920.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(18274..20061)
                     /locus_tag="IW735_000904"
     CDS             complement(18274..20061)
                     /locus_tag="IW735_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043921.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(20074..21027)
                     /locus_tag="IW735_000905"
     CDS             complement(20074..21027)
                     /locus_tag="IW735_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1043922.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(21212..21562)
                     /locus_tag="IW735_000906"
     CDS             complement(21212..21562)
                     /locus_tag="IW735_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1043923.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            21762..22841
                     /gene="pepA"
                     /locus_tag="IW735_000907"
     CDS             21762..22841
                     /gene="pepA"
                     /locus_tag="IW735_000907"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1043924.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            22984..23304
                     /locus_tag="IW735_000908"
     CDS             22984..23304
                     /locus_tag="IW735_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1043925.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            23326..23790
                     /locus_tag="IW735_000909"
     CDS             23326..23790
                     /locus_tag="IW735_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1043926.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            23986..24591
                     /locus_tag="IW735_000910"
     CDS             23986..24591
                     /locus_tag="IW735_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1043927.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(24630..25919)
                     /locus_tag="IW735_000911"
     CDS             complement(24630..25919)
                     /locus_tag="IW735_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1043928.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            26347..26826
                     /gene="rlmH"
                     /locus_tag="IW735_000912"
     CDS             26347..26826
                     /gene="rlmH"
                     /locus_tag="IW735_000912"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1043929.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            27179..27493
                     /locus_tag="IW735_000913"
     CDS             27179..27493
                     /locus_tag="IW735_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1043930.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            27509..27895
                     /locus_tag="IW735_000914"
     CDS             27509..27895
                     /locus_tag="IW735_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1043931.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            27924..>29050
                     /locus_tag="IW735_000915"
     CDS             27924..>29050
                     /locus_tag="IW735_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1043932.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
BASE COUNT         9195 a         5449 c         5877 g         8529 t
ORIGIN      
        1 tgcttccgcc gtttatacgt gtttagagcg tgggataaaa gtgttcttta cttttgtccc
       61 acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa
      121 tccttcactg gtccggaaaa gatcagtgat tgttggtgta gggccgcggt attcgttgcg
      181 ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt
      241 tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cactgcattt
      301 gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg
      361 atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc
      421 cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag
      481 cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg
      541 agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct
      601 gtttgagtct cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata
      661 accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata
      721 gcttcttggg ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga
      781 ggatattctt gtcgcataat cgtataagta tcggctaaag aagggtcttt caaggcagcg
      841 ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg
      901 ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt
      961 agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa
     1021 gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta
     1081 gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga
     1141 catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa ggaaggatgc
     1201 cagatttctg gtcaacaatt acgattccgc aatcgccacc acctgcacca gaagattttg
     1261 cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga
     1321 cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt
     1381 ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac
     1441 tgttttttaa gaaaagctga taagccacca ttttgtcttc tctcgaacgg tgaacttgat
     1501 cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt
     1561 caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt aactcactga
     1621 tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc
     1681 agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat
     1741 tggctagtgc tgcaatcttg aaaatctcca attgagataa attcaacgcg taaaaaacat
     1801 tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc
     1861 catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt
     1921 tttcttgtgc atacttttca gtcaaacgaa tagcagcaag gatataatga aaaggatttt
     1981 cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat
     2041 attgtgcaga ttggatactt ccgacttttc gtgcggattc tacagtgact gttacgaatt
     2101 gatcgactgc agcgataact gctggatgtc ctgtttcaac aactgcatat tctccggcaa
     2161 tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg
     2221 caattcctgg accagcatgg gcagtaatta cttgttcttt gctaaataat ttggtaaatg
     2281 tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat
     2341 ccatcgtaaa gtagcatgga atgccttttt ttctcaattg ccgcaccgca tccatcgctt
     2401 ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca
     2461 tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg
     2521 cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg
     2581 atgtttcgac tgttcgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga
     2641 tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt
     2701 aagaacttaa gtcgtcatgg cctttttccc attcaacgaa accaccgaaa atacttcggc
     2761 aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcgtct aatccaagct
     2821 taagagcagt attgcaagct cctgctaaag cagctagccc gctggcagag gaggctaatc
     2881 cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt
     2941 ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgtaagg
     3001 tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg
     3061 tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat
     3121 ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa
     3181 acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt
     3241 gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg
     3301 cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat
     3361 agattaagaa aatcattgct gattgtcagc ttttccagaa gtgactgtga ttcatccatt
     3421 gtctgtgcca acgtttctgg tgaattctca atgattgctt gttttgcttg cttcgtcaag
     3481 tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg
     3541 acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa
     3601 gcatcgatgt tcaaagaaaa gtagtcaaaa ggatgccctt ttgtaaaata aatcggctgc
     3661 aagctgctag tagcggctgc atcgattcca ctaggattac cgtgggcgat tttttccgaa
     3721 agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa
     3781 gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc
     3841 gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg
     3901 aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag
     3961 gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt
     4021 gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat
     4081 atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg
     4141 taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat
     4201 ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca
     4261 gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg
     4321 actttttttc ttctttgact agaaaaaata caattgacac tactagatat tgggggtata
     4381 tttaaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
     4441 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
     4501 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
     4561 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
     4621 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
     4681 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
     4741 ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
     4801 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttagagcgta
     4861 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga cccaagtcta tacgattatt
     4921 acaaagaatt gaacagacgt tatgatcaat ggtatgaaga atacaaagaa agtccaaaaa
     4981 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
     5041 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtatcc atttgattta
     5101 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
     5161 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
     5221 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
     5281 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
     5341 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
     5401 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
     5461 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cggatgccag
     5521 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
     5581 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
     5641 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
     5701 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcactggct atttatcgca
     5761 agttgtcaga aaaggaaaaa gaagagttgg acgatgcact gatcgaagca cgacaagcag
     5821 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
     5881 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
     5941 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
     6001 ccgttttcgg atgggaaatc gccttgatca tcagtttggt cttatctatt tggatgctta
     6061 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
     6121 gatcacaagt gcttgctctt gattgacgat tagtcccgcc aaagaatctt gagataaagc
     6181 gattgttgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
     6241 cgaaccggtt aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
     6301 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
     6361 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
     6421 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
     6481 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaacc
     6541 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
     6601 gagatagctt tggttacctc cgttgatcag aagaccagag aagatctcct catcttctaa
     6661 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
     6721 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg caagaaagcc
     6781 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
     6841 attagttttt gctatactat aatggttgag aaataaaagc agaagcaccc gcttctcgcc
     6901 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
     6961 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
     7021 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
     7081 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
     7141 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
     7201 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
     7261 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
     7321 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
     7381 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
     7441 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
     7501 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
     7561 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
     7621 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
     7681 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
     7741 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
     7801 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
     7861 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
     7921 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
     7981 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
     8041 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
     8101 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
     8161 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
     8221 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
     8281 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
     8341 ataacgtata agtctaaaat aacataatta aggtttttta attgaaaact gaacatttat
     8401 ggtcatcctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
     8461 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
     8521 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
     8581 atggtcggct tttgtcatca tggctactgt atggatggga ttagcgctag tcggaaacac
     8641 gttttcacca aactcggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta
     8701 tacgacgggg ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt
     8761 tttcgggcgt gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga
     8821 acttggaggg gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat
     8881 attcatgtcg atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccgat
     8941 cttgatgtca ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat
     9001 ggctggaatc tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt
     9061 caatccagcg gctgaaagct atacttataa tgactatttc caaattggta tgattgggat
     9121 gattgtaagc ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg
     9181 ctatgcaatg gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt
     9241 agccgtatta ttaccagttt taggggtcgt tcttttagat cttcaaatca tcttaggttt
     9301 tattctagca ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga
     9361 aatctgtcgt cagtttgcga aattatttac agatggcgca gtcgatgttg caccaatggt
     9421 tggtttcttg atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt
     9481 ttcagctatt tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc
     9541 tatattgaca ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc
     9601 tattttagct gtcgtgttag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt
     9661 atttgcaatt acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg
     9721 gggattaggt tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg
     9781 ttggatcatc ggtgcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta
     9841 aacaaaacgt cgataaaaac gattagataa atggaggaaa agaaaatgaa acgttttgtg
     9901 cgaatgggaa ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg
     9961 cacgaaatca tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc
    10021 gcaggtgtcg ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat
    10081 gttgtgtttg ttgcacattc taccacacaa gcgactaatg cactgattga aggagatgta
    10141 gcaaaagtcg gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga
    10201 caaactagat tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca
    10261 ttcttacctg tcaaaaactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa
    10321 agagaaagag cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca
    10381 gaacgagttg tttatgaagc agcatctgtg aaagcgatcc ctacaacgat ggcttctgac
    10441 atcacaaaat tatatggatt gacaagaaga actcggacag cagcaatcaa cgcatctatt
    10501 ttaccaaaaa tgctagatac ggcaacctcg acagaagact ccgtacgaga agcaggggtc
    10561 aatgtttctt tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa
    10621 aaacgacctg ttttaaccat gctttccggt cctgctgcat cagtcatggg ctctttgatg
    10681 tacttgagag cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg
    10741 gtcattaaaa atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat
    10801 atctcttcat tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat
    10861 cagtcaggaa tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca
    10921 gtatttacgg agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa
    10981 ggagaccctg ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca
    11041 aatacatgtg cagccaatgt tcttggatta gtgcaagaag aacatttttc ttatggaaat
    11101 gtcccatctg cacgaaaagc catacaggca ttagcagact attgtcatac gacggttgaa
    11161 gacatcgcag agcagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa
    11221 ttggcagaca agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc
    11281 gctgcctcat tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa
    11341 aacgccgaag tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa
    11401 cgtatcatcc cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat
    11461 aaagcgattg aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca
    11521 catacttcaa aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta
    11581 accaaggaga tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat
    11641 aaaaatgaag tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat
    11701 cgttcaaaaa atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa
    11761 cgtggacatg cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa
    11821 ctttgggaag atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc
    11881 tgcttaggag caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg
    11941 atggatctgg aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat
    12001 atcaaacaaa cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat
    12061 ttggggattc taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca
    12121 gaaaattctt atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa
    12181 aaagtaccct tttgccgatg taggagaaat ctatgaacaa atggctgttc caatcgtttc
    12241 ctgtgaatca gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg
    12301 gatccgattg atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc
    12361 taaagaaaca gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc
    12421 tgttaaggga atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac
    12481 acatcaatca aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat
    12541 gaatcaaagc gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga
    12601 aattcgtagc caagggatct tagaagaaat aaaagaaata gaaaaaagat gtacggagta
    12661 tgatttagat tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg
    12721 aataaacggt gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga
    12781 gaagctgttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
    12841 ttggaaaaag gtgttcaaaa gcttgcatct gcggtaataa gaacagccaa gttaaaaaca
    12901 taagaagcta tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac
    12961 ctctcaataa acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa
    13021 atatgaggaa ctattttgcc gtttatttct ctaatactca ggtcaaaacg ctgattccaa
    13081 aacctcttat ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat
    13141 cacgtattct tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc
    13201 atcaaagtta tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc
    13261 ccagtctaat tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata
    13321 actttctagc caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa
    13381 atcagtatcc aaatcaaaaa aagctttttt attttgagcg atcactgcct gatcggtttg
    13441 ttctagtatt ttttctagtt tagcttgatc ttgcttggtc attttcgcac tgcattcttg
    13501 caggatcggt ttttccaact tttcccgcgt ataacgggct aatgaagcag atcggatatc
    13561 gatttttgaa acataagtgc cggactgtgg aatggttttt acaagctctt gattgcgcag
    13621 ttggatcagt gcttctcgaa tcggtgtacg cccgatagct agttgttctt ctaacacttt
    13681 atcggagatt ttacttccag gtgctaactc ggaataaatg atttgtttac gtatagattc
    13741 ataagcttgc tgttgcaaat tattgataac catttaacct tcctttctat gcagatataa
    13801 tctattataa cataaatcgc ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa
    13861 agccctgata ggcatgccta ttagagcctc gaaaagaaaa atattgaatc aatctggtcg
    13921 tacctcttgg atcggtttga cacgaccgcc gattctttta gcaaaagctt cggattcttt
    13981 tttgtctttg aaaatgagca gattgtctaa gtttttttcc cgagtgcgac agccagtttt
    14041 atcgatgtaa ccgtgcatta ctttaacgac atacatgggg atcacctgct tttttctttt
    14101 cattatacga actactcgac acaatacaag aaaaatgctt agacggtatt tattaagaat
    14161 ttacatcatc caaagaagcg tacgcttgga ataactgttc taactccgga ttgactttgc
    14221 tcagcttcac taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag
    14281 tttttaattt tttgtcttca gttcccacaa tttcgtagct aaaatccaac cgtgttcccg
    14341 tatacttttg gatagttggg tggatgctca ctttttctcc gtaatggatc atttctttat
    14401 agctgcaatt tacttccaga acggggatga tgatcccaga ttcttcgatt tttttatagg
    14461 gaaaccctag atgtaccaat aaggctaccc gagcctcttc aaaccagcgg atataattgc
    14521 tgtgatgaat aatccccatt tgatctgttt cataataaaa aggttctcgc acataacctg
    14581 taaatttctc catgactcat gcctcgtttt ccattgattt tttacaaaaa taattcatcc
    14641 acttcggaag tgcttcgctg atttgtgtcg gcaaaacaat atattgttct tttgccagct
    14701 ggtctccaat gaaactatga agataaacag ctgctgcaat cgtttggtca ttttgaggaa
    14761 actgggctaa aaatcctgtg atcatcccag ctaaagtgtc acccatgccg cctgttgcca
    14821 tagcagcaga acctaaagga ttggtgtaga agtgctggtg accataaatt tttgtctcat
    14881 gacttttcaa aacgagcaca gcaccgattt cctgttgctt ctttttgttt tctttatccc
    14941 gttgttcttg tatcggtaga gaactcaacc gttgccactc catttgatga ggtgtaagta
    15001 cagtttgttc gggatagggt agcaaaagat gattagcaac aagtaaactt aaagcagagc
    15061 catcaatcac cagccattgt tcttttgatt gtttttctag tacttgtttg aaacgttcca
    15121 aactcttaga agaagtaccc aatccgggac caatcaagag tacatctgct tgctttatta
    15181 cggaaagaaa ctggtcagta tccgtccaat ctacaatcat tgcttctggc agatgactgt
    15241 gaagagcact ccgatttttt tcatcagtta ctacagtcgt gagacccgct ccactgtgta
    15301 cggcagctaa agcactcata atgattgctc cgccatattg ttcattgccg ccaatcaaaa
    15361 cgatacgtcc aaatgttcct ttatgtgaaa tctttggacg aggtgtgatg actcttttca
    15421 ctagttgttc gtttatcagc atcgaaaaac ctccttttac tcattatagc gtatattttt
    15481 tttagagaaa aacagataag aaaaggaatt gtcagatttt ttgaactatt tttttctgta
    15541 atttgtggta tcatgaacac gaagacttaa ataagtggag gattaacttt atgacaattg
    15601 attggcaaaa agaagtagaa gcacgtaaag aagacttgct tgaagacttg aaaaatcttt
    15661 tacgtgtcaa cagcgagcgc gatgattcaa aagtaacacc agatgcacca tttggacctg
    15721 gacctcgcga cgcattgaaa catatgttag cttatggcga acgtgatgga tttaaagtaa
    15781 aaaatgtcga taactatgca gggcatattg atcttggcga aggagacgaa acgctaggga
    15841 tatttggaca catggatgtc gttcctgcag gagatggctg ggacacagat ccttatgaac
    15901 cagtaatcaa ggatggaaag atctttgcac gcggatcaag cgatgacaaa ggtccaagta
    15961 tggcagctta ctatgcgatg aagatcatta aagaacttga cttgaaacta tcaaaaaaag
    16021 ttcgcttcgt agtcggcagc gatgaagaaa gcggctgggg cgatatggct tattatttcg
    16081 aacatgaaga agaaccagat tttggtttct caccagatgc tgaattccca atcatcaatg
    16141 gtgaaaaagg aaatgtttct ttagctttac gcttcaaagg cgacaatgca ggagattatg
    16201 tcttgaaatc ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca gcaactgcag
    16261 cacttcaagt accaagtgca gatgcagcta tcgcgatgga agaagcattt taccaattca
    16321 tcgaagcaaa tccagtaagc ggaacgattg aagcggacaa tacgtatgta aaaatcgaat
    16381 tagttggtaa aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca ggttctttct
    16441 tggcattgtt cttagataac tatgaattcc taggatctgc taaacaattt atccatgtag
    16501 cagctgctta tgtccatgaa gatttttatg gagaaaaact aggagtcgct tatgaagatg
    16561 aaaaaatggg caaattgaca atgaatgctg gactatttgc ttttgaagaa aatggtacag
    16621 aggaagcgaa cttcatcaat attaatttcc gttttccaaa aggtgtaaca gtagacggat
    16681 tgcaaagtga catcgaacaa acagtggggc aagaaggcgc aacagttact cgaggtgctc
    16741 gcgtaatgga accacattac gtaccaatgg atgatccatt ggtggctaca ttattgcaag
    16801 tctatgaaga ccacactgga gaaaaaggct acgaacaaat catcggtggc ggaacttacg
    16861 gacgtttgtt gaaacgtggc gttgcttatg gagcgatgtt cccaggatat actgatacaa
    16921 tgcatcaagc aaacgaattt atgagtcttg atgacttgtt cagagcaaca gcaatctatg
    16981 cagatgccat ctatcgtttg gcgaaataaa aatactaaaa aactccggct atctctgaca
    17041 gctggagttt ttatgtttaa aaatgaatta gtacatcggt attcttaaaa gtaatttagt
    17101 tcgtcttttg atttctcagg agggatcaaa aaggcgaaag cactaagtat agcaaaactt
    17161 gtgatgattt gcgtacgata cgtaaaccaa taatgaatct gtgagtgatt agctaaaata
    17221 ttgtaccaaa tatacggcgt tacagcagct aaaacaaaca aaactagaag ttggaacgga
    17281 cgccagctat ttcgtttacg ataagcaaaa tagagaaaaa caagacaagt aatcaatagc
    17341 aataatattg ctacttttgg atacatcaat ctaatattgc ttttcagcat ctctaatcga
    17401 tgcaacgggt atttgtcgtt tccttccgtt cgaaagagga tttggttgat tgcatctttt
    17461 acaatgtttt tgttcaatat gatcgatgat aggatccatt tagccaccca tgtcaaacca
    17521 tagcctaatc cccaaaagat accggtaaaa acaaaactgc ctacttgttt gctcaatgaa
    17581 ctgcgtttat ccattaaata cttatttgtt acataataaa taatgatcac aggtatcccc
    17641 caagagatga ccggtgttgt caacaagtca aaaaagttag ctaaagaacc aatgacaaag
    17701 aaatacaaga ataattgttg gttggtgttg gagagttgtg gttttgttaa aaatagtaat
    17761 acagacaata aagtcaaaat ccaaatattt gaaaattgca tactgaagaa aaaggttgca
    17821 gcatttccta tagcaagcga aatcaacaag gctgtcgtta aatagatgtc gctacacttg
    17881 gttaaaaagt agctcgtcaa tccaatcagc agaaaaagaa tggctgcgta aatcattcga
    17941 atactcccca aactcatccc tagcagtaaa ggtcgtaaaa aaagctgata accatgccaa
    18001 taacggggat aatccgccat agacatcgcg ttctccaagg agttcaattc aggatctgct
    18061 tttgtttttc gtatcatcaa atgatcagta aaattatcta accgagtgcc tagcatattt
    18121 ccttgattga tagaaggata cattccttct ttttcaacca tctgtagaga tttttctgca
    18181 ttcgattcaa tagagtgctg cggaatgaaa aaagtaattg tcagtagtac gcaatagcta
    18241 gcgatcaaga gaacaaaagc aaaaaatatc tttttaaata gtttcatgag tatctcctat
    18301 atctgttttc attttttctc ttttatctag taaaaaagga gagaggatat aaacaggtag
    18361 acctaatgct aaaacaaaca cataccgtaa actaaaagcc acaggagtag caatcataat
    18421 agtagcaaaa acaagcatac cgggaagaaa aaggagtaaa taccgatatt ttttcttaaa
    18481 gattgctaaa agtgcactgc cgatagctaa ccacagtaat gtgccggaac caaagaaatc
    18541 acgagcatcg atgatttttt tcattcctga caatccagta agatgttcaa tcaaatctac
    18601 ttgcttcata ccataattat tcgtgtttac acgagtatct aaaaagccgt aactattttt
    18661 tgtaccaggt acccagaacc cgaacgtttc taaaatataa gcatctgtat agatttttat
    18721 attacttggt aaatttcttg cccatagtga tagaaattct tttttatgtg tattcaaaaa
    18781 ctcattgtta aaatttttat gccacttgat tgagtctgca agactaggag aatacgcttc
    18841 ataatctttt atcgggagta gctgattcat gaatgcttta tctttcttag acaagtcacc
    18901 atccataaca atagttctag aaatttgctg caaaggaaca cctaattgtt cagcagtttc
    18961 tgtaggaata gcataacgag ccatcaacgg gtggatgagt agtccagcaa tcactgtata
    19021 acccagcacc acttgaaaga atgcttgata ccttttttgt ttcaaaataa tcaagccgca
    19081 aatcaaacct gtgccggtta ctacataaat cccattattt cttagatagg tggtcagtaa
    19141 agcaaggatg attacgtaaa tctgattgag aatcgttggc ttttctcgtt gaaaaataaa
    19201 gtcaaaaacc gctaaggaaa ataaaaagca agcgatagag aacaaagtat ctttttgcat
    19261 gttcaaggcg tagattggaa aaaccggtgc aaatgcaaaa tagcataaac taatgagtac
    19321 cggaaaaaaa tggatgtttc tttttaacag ccagcaaaga gaataactta tcgctgaagc
    19381 tgttatcaat gtttgaaaaa tcgaaaataa aaatacaccg tgattgatcg tatcagttgg
    19441 taaatcatga acaaatagct tgaccagtaa ggtgaataag actggatgat gattgcttat
    19501 aggaacatca cccatagatt gggcgaccga acttaacgaa tcaggcaaaa ccactccagg
    19561 atacaatgcc aacagataag gaagccaaca cagaactaaa aagacagtaa agaataaaaa
    19621 atattttctt gaagagaaag cctgtgtgtt ttgagcagta aaaaatgttt ccagttttgg
    19681 cattgataaa taaatcgatt gtaaaaataa aaaagaatac acaaaaatgg agaaataaac
    19741 aagaaagtaa aaaatacttc catcagcaat cgttgtcttt gtatagtttg ctgagacata
    19801 ctgaaattgt atctgtctgc caatagaaag tgtaagagca aaagctgcag aaaaaaacaa
    19861 tcgggcaata gctgcaactt tgttttttat tgaaaacaac tctattccat attgcttaaa
    19921 agcaagtagc aaaaccatgg ctaaaccaat gattactgtt ggattcaaag ttcctagacc
    19981 ataagaagta aaaaaagcaa aacctgacgc aaataaagtt gcaaaaaaag taggaaatag
    20041 attctccttt ttcttatgca taaaaaaggc ctcctacttc atttctttat tttgatattc
    20101 gtggtagaca tggtataaat tcaattcata ttctttcttg gcatttgtga cgaccgtatc
    20161 caaaatcaac cctgttacta atgaaagaag ggctaaagtc atcaatccag tacttaaaat
    20221 cgcagaaggg atgcgtgtaa taaagcttgt cagcatatat tcacgaatca caggaatccc
    20281 catgaccaat ccaaataaaa agaagaagat ggtccagatc ccaaagaaaa gcaaaggttt
    20341 ataatctttg cacattttga tgatcatcat gatcactttg aatccgtcag agaatgtatt
    20401 caattttgat tcgctacctt caggacgatc gcgataatcg attggaatct caacgaactt
    20461 gaatttttta tctaaagcat gaatcgtcaa ttctgtttcg atttgaaaac cagaactcat
    20521 aatagggaag cttttgacaa aaattcgatt gaatcctcga tagccggtca tgacatcgcg
    20581 aatttttgtt ttatataagt aagtaattgt atttttaacc agattattcc caaaatcatg
    20641 gaaaggacgc ttattttcat caaaataggt tccatttgat agacgatcgc caatgaccat
    20701 atctgcttcg ccagaacgta atttctcaag caaaccatgt gcagcttctg caggataagt
    20761 atcatcacca tctaccatca aataataatc ggcatcaata tcaaaaaaca tttgacggat
    20821 tacattccct tttccttgac gtggttcttt ctttacgatt gctccgcctt gaacggccaa
    20881 ttcgtatgtt cgatcggtcg aattattatc atagacataa atatctgctt ctggtaattc
    20941 ccttttaaaa tcttcaataa cagttgaaat tgtcgcctct tcgttataac aaggaatgag
    21001 cacggcaatt ttttcacgtg attccatttg tcactctcct tatataataa aactaatctt
    21061 atttcaataa taccgtataa taactacaaa tactagctat tccaagagga aatagcccta
    21121 tttttataaa aatcacccac tataaaagaa ggataggata cctctctttt agatagtggg
    21181 tgcgtattcg ttatttcatt tttttgacgt attaatctat tgcgcccttt tttcttgaaa
    21241 tgtctaatac ggttcctgta tgagcatctg ctaaaaattc ataataaaca gtttcgccat
    21301 cttctatccg ggaaagccca ccccgataag cactcgaccg aacggcgaat ttccgaatag
    21361 gttctttttc aaaagagatc caagcacctt cgattgcgcc ttcttttaaa aaagatgttt
    21421 tgatattttc aagcacttgg tctgctgata acactttttt gcgatgatac caaatcgttg
    21481 aagctacccc actcaataat cctagaccga tcccaacggc taacccgcct ttaaaatagc
    21541 ttaattcgct ttcttcatac atgtggatat acctccttct tctcacttgc tactctttgt
    21601 gtttatttta ccacactctc atattttctt tgaaataaat aaccgctacc agaaaaaata
    21661 agctataata aatacgatcg gtctgcagaa aaaatcatat tttatccgca gatcaatata
    21721 agtctgagag gagtttagtc tgaaatttag acgaatagca tatggaagaa aaaacatttc
    21781 aacgaatcaa agagttaaca gaattacaag gaacaagcgg ttttgaacaa gacgttcgcg
    21841 cctatatgga aagtcatatg gaaccgctgg tagatgagtt acagttagat ggtttgggcg
    21901 gcatttttgg gctgcgccac catgaagaag cggatgcacc tcgtgtaatg gtcgcagcac
    21961 atatggatga agtcggattc atgctgacac aaatccaaga aaacgggctt tttaaagtcg
    22021 tacctctagg cggatggaac ccttacgttg tttctgctca acgttttaca cttaaaacct
    22081 ctactggtaa gaattatcca tgtatttctt cttccgttcc cccacattta ttacgaggaa
    22141 caagcggaca aaaatcagta gaagtaacag atattttgtt cgatgctggg tttgaatcaa
    22201 gagaagaggc aatgagttac ggtgttcttc caggagacac aatcgttcct tacgctgaaa
    22261 cgatcaaaac agccaacggg aagaatatca tcagcaaatc gtgggacaac cgctatggct
    22321 gtacaatggt attagaagct ttagaagcgt tacaaaatga aacattaggt catacattga
    22381 ttgcaggcgc gaatgtccaa gaagaagtag gcttgcgtgg ttctaaagct tctgtcaata
    22441 aattcaagcc agatctgttc tttgcagtag actgctctgc agcagatgac acagtgacga
    22501 aaaatggcac attcggacat ttaggagaag gtacattgat gcgtatccaa gatccaggtt
    22561 tgatcatgct tcctcgtttg cgtgagtact tgctagatat cgctgaaaca aacaacatcc
    22621 cttatcaata ctttgtttca aaaggcggta cagatgcagg tgctgctcat acacaaaatg
    22681 aaggaatccc aagtacagtg attggcgtag tgggacgtta tatccatacg catcaaacga
    22741 tgttcagtat tcgcgatttt gaagctgcac gagaaatgtt gattcaaaca ttgaaaggtt
    22801 tagataaatc aacagtgaac acgatcgtat acgggaaata acgcgatagt gaaactataa
    22861 aatctggcaa aagcaacctt ttgtcagatt ttttttataa aaagaggatc aatgtttgtt
    22921 agttcgaaag aaaggtgtat aatttaattg aaaaatacat aaacagaaag tagaggaaaa
    22981 agaatgatta tcccaaatag tttagaagaa ttagcaggct acgtagaaac tggaaaaagt
    23041 gtatttttct ttactgccgg ctggtgcgga gactgccgtt ttatcaaacc gcaaatgcct
    23101 gaaatcgaaa atgacttttc tgattggcaa tttattgaag tagaccgtga caagtacata
    23161 gatgtagccg ctgaatggaa catttttggt atcccaagtt ttgtcgtgat ccaagacgga
    23221 aaagaattag gtcgtctagt gaataaagat cgtaaaacaa aacaggaaat cgaatcgttc
    23281 ctagaaagtg taataaaagg ctgacatctg ttaggagggg tcaagatgaa tcaagaatat
    23341 aagaacattt tagtaggtat tgacggtagt gaacaagcac ttgctgcttt caaaaaagcg
    23401 ttagaagtgg ctcgccgtaa taatgggaaa gtatatgtag caaacgtaat cgatcagcaa
    23461 ttttataatt ttatgggcta tgctcctatt gatcaaaatc tagtcgatca gcaaactgaa
    23521 acagccaagc aaatgatcga agaatgtaaa gaatatggaa aatctgttga ttacacaaat
    23581 atagaaggaa ttattgcata cggctctgtt cgtgaatcca tggcagtcaa attgccaaaa
    23641 aaatatgcaa tcgatctaat catggttggg cagtctggac taaatgcagt cgaacgcttt
    23701 atgacaggaa gtgtagccag ctacgtcata cgtcattcac cttgtgatgt cttgatcgtt
    23761 tctgacgaag aagaacagcc aagtaattaa gcaagtggaa cttgtataac ctttttaatc
    23821 aaacagtaaa gggactgtga caaaagtctt gtcacagtcc cttgttacga ataaatggtg
    23881 ttccagaaga agcttcttcg gaaataaagc atgaaaaaaa tctcatgcag tgaatgcaat
    23941 tttttgttaa gataaatctc agcaaaggat aatggaggaa agaacatgat ttttgcttat
    24001 aacaaagcac acgtcggaga cactttgatg gttatcatag ccgacgataa aggaacagaa
    24061 aacactgtcg aacgtaaatg gaatgttgct cggatcaaag atgaacaagg tcaagttgtt
    24121 gcttggaact ttttccatat ctccgatcat ctaacaattg aagggaacgg ccaagtaagc
    24181 ttaacagatg aagaattagc gaaattgaat cgattgatta aagaagcagg attcgaagaa
    24241 cagcttgaag ctgatcatga accgaagatc gttgtcgggt atgtaaaaac atgcgtcccg
    24301 catcctgact ctgaccattt atcgattact gagacagaag tggacaatgg gacagtattg
    24361 caaattgttt gcggtgcacc aaatatcgaa gctggacaaa aagttgtcgt tgcaaaacca
    24421 ggagccatga tgccagatgg aatgatgatc tggccaggaa ctttgcgagg ggtagccagc
    24481 tatggaatga tttgttctgc tcgtgaattg catttgccaa atgctccaga aaaaaaagga
    24541 atcttagtat tgcctgagga tgccgtagtc ggagaagtat ttccaaaata aagaggttgt
    24601 gacaagtctt tgtcacaacc tctttattat taattttcac tattttgatt ttgttttaat
    24661 gccgattgat cgatcgtcaa ttcaacagta gttgtttttt ctttcgagcc ttcatagtaa
    24721 gtgattttga cagaatctcc cacttttttc ttatacaaag cagattgaag ttctacacca
    24781 gaagaaactt ctttgtcatc gatcttagta atgacgtcgt attgtttcaa cccagctttt
    24841 tctgccggtg tagctgtttg gacatttgtc acgatgacac cgttagttac agatgaagga
    24901 atcttcaaga tttgttcttg ttgctgagta gaaacggcag ataagtcgac cattgtgata
    24961 cctagagctg gacgtgtcac ttttccgtct ttttccagtt ggttgatgat attcactaca
    25021 tcgttacttg ggatggcaaa tcccattcct tctacgctta cattagaagt cgattcagaa
    25081 gtacttgcga tcttacttga gttgataccg ataacttgtc cttcgatatt cactaaagga
    25141 ccaccagaat ttcccgggtt gatcgctgca tctgtttgga tcgcattgat attgactgtt
    25201 tcgttcgatt cattcgtact tgttacttgt cggttcaagg aagaaatgat tcctgaagtg
    25261 acagaattcg catattcaga tcctaatggg gaaccaatcg caatcgcagg ttcaccaacc
    25321 ttcaaagcac ttgagtcccc aaaagaggca accgtttcga ctttgtctga attgatcttc
    25381 aacaccgcta aatctgaata ggcatctgtt ccaactaatt cagcttttac cttcgtaccg
    25441 tctttcatca tgacttccaa accttgctgt ccatctacta cgtgattgtt tgtgactaca
    25501 taagcagtat tcccgtcttt tttgtagatt acgccgcttc cttcactata agcttccaaa
    25561 ttactatcat ctgagctgct actttcttct tgctgcccga ataatccgcc aaaaccgctt
    25621 gattggtttt gactttgtaa gttgataaca gaaacgacag cgtcttgaac tttatctacc
    25681 gctttggtga tatcagaatc tacattgact ttgacatttt ccacaactgt ttcaccggca
    25741 gaattttgat tgccgcttgt agcagtagaa ctattgttgc cagtgcccat cgctgcataa
    25801 aaaatgccag ctgtcagtag tcctccaacg atccctccaa caagccccag acccaactta
    25861 cgccagaggc tattattttt attttttttc atctttggtg tcacattttt tctatccatg
    25921 tgttcctcct ccaattacat tcatttatct actgttagta tagtcttatt tgttgacttt
    25981 agtctacttt gagactttga actttttatg aaaaacttta ggaaaaatca ttgttttcag
    26041 atgaaaatat ttgattttct ttttccacag atattgtgta aaactacgcg gataggattg
    26101 gaaaaataag ggataaaaca tatccacagc ctgtgtataa agtggataaa ttcttataaa
    26161 acgtatgttt gcctaatgaa tgtgatgaaa taaggtaaaa caacgctgtt ttctgtggat
    26221 aaacctgtgg atgttgtgca taactgttgg ggaaatcttt ttaacaaaaa tagtcaaaat
    26281 aaacatatag acgaaaaaat tgattataat aaacaaagtg agcgtgaaga tcaaaggaga
    26341 gaaaacatga atatcaaaat tatttcagta ggaaaattaa aagaaaaata cttgatccaa
    26401 ggtatcaatg agtatgtcaa acggctgaat gcctatgcga aaatcgaatt gatcgaagtt
    26461 cctgacgaaa aagccccgga gaatttaagc gaagcgcaaa tgagacaagt aaaagaaaaa
    26521 gaaggagaac ggatcttagc gaaaatcaaa gaacaagaat acgtctatgg cttagcaatt
    26581 gaagggaaaa atcccacaag cgaatctttt gctaaacaaa tcgaccaatt aggtatccaa
    26641 ggaaaaagcc accttgtttt tgttatcggc ggttctttag gactcagcga agccgtcatg
    26701 aaaagaagca atgctcagat ctcttttggc aaaatgacgt atccacatca attgatgcgc
    26761 ctgatcctcg tcgagcaaat ctaccgtgcc ttccggatca acgcgggggc tccataccac
    26821 aaataatgat attttttaag agaaaaatag cataaaaatc tagttatccg cataaaaact
    26881 ggacttatca cactttatca aggtcaaaac cactcaattt actactaatt tactacttat
    26941 gaatgagctt tgatacgacg atttatcctt gaaaagtgaa gatataaaga tacttccaat
    27001 aaaatttgaa tatttaatag gtagacactt caaaaaatga ggtgtctatt tttttacccg
    27061 attttgaaag gaagtgaact tatgaaaaca aaaaatcaag aatcaaaagg tcgttcccca
    27121 ctctttaaga ccatcaaaca ttcattcagc caataaaaaa gaaaggatag gtaaaaatat
    27181 ggaacttaaa tttgtgattc ccaacatgga aaaaacattc ggcaatttag aatttgctgg
    27241 cgaggataaa gtcgttcagc gaagaatcaa cggacggcta actgtcttat caagaagcta
    27301 taatctctat tctgatgttc aaagagcaga tgatattgtg gtggtgcttc ctgctgaagc
    27361 tggcgaaaaa catttcggct ttgaggaacg tgtgaagtta gtcaatccac gtattaccgc
    27421 agagggctac aaaatcggca ctcgtggttt tacaaattac cttttacatg ctgacgacat
    27481 gataaaagaa taaagaaaga gaggaaaaat gatgagatta gcaaatggca ttgtattaga
    27541 taaagacacg acttttggag aattgaaatt ctctgctcta cgtcgtgaag tgagaatcca
    27601 aaatgaagac gggtcggttt cagatgaaat caaggaacgt acctatgact taaaatccaa
    27661 aggacaagga cgcatgattc aagtaagtat tcctgccagc gtgcctttga aagagtttga
    27721 ttataacgca cgggtggaac ttatcaatcc cattgcggac accgttgcta ctgccaccta
    27781 tcaaggagca gatgttgact ggtatatcaa ggcagacgat attgtgctga caaaggattc
    27841 tagttcattc aaagctcaac cacaagcaaa gaaagaaccg acacaagaca aatagtcgct
    27901 aggtagaaag gagacttttt cgcatgaaac agcgtggtaa aaggattcgc ccatctggta
    27961 aagatttagt ctttcatttt acgatagcgt cactcctgcc tgttttcctg ctggttgtcg
    28021 gactgtttca tgtgaagaca atccagcaga tcaactggca ggattttaac ctatcacaag
    28081 cagataagat tgacattccc tatttaatta tcagtttcag tgtcgcaatt cttatctgct
    28141 tgctggtagc gtttgtattc aaacgggttc gctatgatac ggttaaacaa ctttaccacc
    28201 gtcaaaaact ggcaaagatg atacttgaaa acaagtggta tgaatctgaa caggtcaaaa
    28261 cagagggttt ctttaaagat agtgctggtc gtacaaagga aaagataacc tacttcccta
    28321 aaatgtatta tcgacttaaa aatggcttga tacagatacg ggtggaaatc acgctgggaa
    28381 aatatcaaga ccaactctta cacttggaaa agaaattaga gagtggcttg tactgtgagc
    28441 tgacggataa agagttaaag gattcctatg tggaatatac tttgctctat gacaccatag
    28501 ccagtcgtat ttctattgat gaagtagaag ctaaagatgg taaacttcgc ttaatgaaaa
    28561 acgtatggtg ggaatatgat aagctccctc atatgttgat tgctggtggt acaggtggcg
    28621 gtaaaactta ctttatactg acactgattg aagccttgct tcatacagat tcaaaactgt
    28681 atattcttga cccgaaaaat gctgaccttg cggacttagg ttctgtgatg gcaaatgtct
    28741 actatagaaa agaagacttg ctttcttgca ttgaaacatt ctatgaagaa atgatgaaac
    28801 gtagtgagga aatgaagcag atgaagaact ataagactgg caaaaattat gcttacttag
    28861 gtctcccggc acacttctta atctttgatg aatacgtcgc tttcatggaa atgctgggaa
    28921 caaaagaaaa caccgcagtt atgaataagc tgaaacagat tgtcatgtta ggtcgtcaag
    28981 ctggcttctt tctaatactg gcttgtcaac gtccagacgc aaaatattta ggcgacggaa
    29041 tccgtgatca
//
LOCUS       DACEMS010000023        28804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEMS010000023 DACEMS010000000
VERSION     DACEMS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.023"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(105..629)
                     /locus_tag="IW735_000916"
     CDS             complement(105..629)
                     /locus_tag="IW735_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1043933.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IW735_000917"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IW735_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1043934.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IW735_000918"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IW735_000918"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1043935.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IW735_000919"
     CDS             complement(2351..3031)
                     /locus_tag="IW735_000919"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1043936.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IW735_000920"
     CDS             3157..4026
                     /locus_tag="IW735_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1043937.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IW735_000921"
     CDS             4052..4567
                     /locus_tag="IW735_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043938.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IW735_000922"
     CDS             complement(4614..5177)
                     /locus_tag="IW735_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1043939.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IW735_000923"
     CDS             complement(5232..5411)
                     /locus_tag="IW735_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043940.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IW735_000924"
     CDS             complement(5488..5997)
                     /locus_tag="IW735_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043941.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IW735_000925"
     CDS             complement(6093..6473)
                     /locus_tag="IW735_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1043942.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IW735_000926"
     CDS             6803..7552
                     /locus_tag="IW735_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1043943.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IW735_000927"
     CDS             complement(7736..9013)
                     /locus_tag="IW735_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043944.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRNTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IW735_000928"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IW735_000928"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1043945.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IW735_000929"
     CDS             complement(9935..10879)
                     /locus_tag="IW735_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1043946.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IW735_000930"
     CDS             complement(10881..11567)
                     /locus_tag="IW735_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1043947.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IW735_000931"
     CDS             12041..12361
                     /locus_tag="IW735_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1043948.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IW735_000932"
     CDS             12515..13420
                     /locus_tag="IW735_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1043949.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IW735_000933"
     CDS             13417..14190
                     /locus_tag="IW735_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1043950.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IW735_000934"
     CDS             complement(14371..15795)
                     /locus_tag="IW735_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1043951.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IW735_000935"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IW735_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1043952.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IW735_000936"
     CDS             complement(16517..18499)
                     /locus_tag="IW735_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1043953.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IW735_000937"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IW735_000937"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1043954.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IW735_000938"
     CDS             complement(19412..20473)
                     /locus_tag="IW735_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1043955.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="IW735_000939"
     CDS             complement(20684..21211)
                     /locus_tag="IW735_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1043956.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /locus_tag="IW735_000940"
     CDS             complement(21278..22780)
                     /locus_tag="IW735_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1043957.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="IW735_000941"
     CDS             complement(23038..24159)
                     /locus_tag="IW735_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1043958.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="IW735_000942"
     CDS             24285..25226
                     /locus_tag="IW735_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1043959.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="IW735_000943"
     CDS             complement(25376..26272)
                     /locus_tag="IW735_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1043960.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="IW735_000944"
     CDS             26541..27881
                     /locus_tag="IW735_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1043961.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="IW735_000945"
     CDS             28099..28542
                     /locus_tag="IW735_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1043962.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT         8297 a         5975 c         5173 g         9359 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtat
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatgaca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgttttt
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atgg
//
LOCUS       DACEMS010000024        28188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEMS010000024 DACEMS010000000
VERSION     DACEMS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.024"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(76..543)
                     /locus_tag="IW735_000946"
     CDS             complement(76..543)
                     /locus_tag="IW735_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1043963.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="IW735_000947"
     CDS             complement(663..2201)
                     /locus_tag="IW735_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1043964.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="IW735_000948"
     CDS             2281..2967
                     /locus_tag="IW735_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1043965.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IW735_000949"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IW735_000949"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1043966.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IW735_000950"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IW735_000950"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1043967.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="IW735_000951"
     CDS             complement(4453..5583)
                     /locus_tag="IW735_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1043968.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="IW735_000952"
     CDS             complement(5583..7766)
                     /locus_tag="IW735_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1043969.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="IW735_000953"
     CDS             complement(7763..8788)
                     /locus_tag="IW735_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1043970.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="IW735_000954"
     CDS             complement(8833..9660)
                     /locus_tag="IW735_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1043971.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="IW735_000955"
     CDS             complement(9657..10724)
                     /locus_tag="IW735_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1043972.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="IW735_000956"
     CDS             complement(10750..11586)
                     /locus_tag="IW735_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1043973.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="IW735_000957"
     CDS             complement(11583..12452)
                     /locus_tag="IW735_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1043974.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="IW735_000958"
     CDS             complement(12522..13805)
                     /locus_tag="IW735_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1043975.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="IW735_000959"
     CDS             complement(13819..15504)
                     /locus_tag="IW735_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1043976.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="IW735_000960"
     CDS             15779..16804
                     /locus_tag="IW735_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1043977.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IW735_000961"
     CDS             complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IW735_000961"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1043978.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IW735_000962"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IW735_000962"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1043979.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="IW735_000963"
     CDS             25476..26444
                     /locus_tag="IW735_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1043980.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="IW735_000964"
     CDS             26462..27367
                     /locus_tag="IW735_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1043981.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="IW735_000965"
     CDS             27364..28170
                     /locus_tag="IW735_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1043982.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT         7781 a         6134 c         5317 g         8956 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctccct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgagaa ggagtaacaa ctgatttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttgcttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggac
//
LOCUS       DACEMS010000025        27999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEMS010000025 DACEMS010000000
VERSION     DACEMS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.025"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..586)
                     /locus_tag="IW735_000966"
     CDS             complement(5..586)
                     /locus_tag="IW735_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1043983.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IW735_000967"
     CDS             complement(769..1395)
                     /locus_tag="IW735_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1043984.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IW735_000968"
     CDS             complement(1417..2748)
                     /locus_tag="IW735_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1043985.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IW735_000969"
     CDS             complement(2903..3304)
                     /locus_tag="IW735_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1043986.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IW735_000970"
     CDS             complement(3390..3596)
                     /locus_tag="IW735_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043987.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IW735_000971"
     CDS             complement(3691..5193)
                     /locus_tag="IW735_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1043988.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW735_000972"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW735_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1043989.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IW735_000973"
     CDS             complement(5764..6381)
                     /locus_tag="IW735_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043990.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IW735_000974"
     CDS             6602..7975
                     /locus_tag="IW735_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1043991.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IW735_000975"
     CDS             8050..9162
                     /locus_tag="IW735_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1043992.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IW735_000976"
     CDS             complement(9234..10871)
                     /locus_tag="IW735_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1043993.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IW735_000977"
     CDS             complement(11039..11647)
                     /locus_tag="IW735_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1043994.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW735_000978"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW735_000978"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1043995.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IW735_000979"
     CDS             complement(13201..13953)
                     /locus_tag="IW735_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1043996.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IW735_000980"
     CDS             complement(14094..15044)
                     /locus_tag="IW735_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1043997.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IW735_000981"
     CDS             complement(15045..15884)
                     /locus_tag="IW735_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1043998.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IW735_000982"
     CDS             complement(15881..16627)
                     /locus_tag="IW735_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1043999.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IW735_000983"
     CDS             16865..18235
                     /locus_tag="IW735_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1044000.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IW735_000984"
     CDS             complement(18290..19186)
                     /locus_tag="IW735_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1044001.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IW735_000985"
     CDS             complement(19467..20144)
                     /locus_tag="IW735_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1044002.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IW735_000986"
     CDS             complement(20235..21140)
                     /locus_tag="IW735_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1044003.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IW735_000987"
     CDS             21315..22769
                     /locus_tag="IW735_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1044004.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="IW735_000988"
     CDS             22785..23816
                     /locus_tag="IW735_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1044005.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IW735_000989"
     CDS             23823..25010
                     /locus_tag="IW735_000989"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1044006.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IW735_000990"
     CDS             25166..26548
                     /locus_tag="IW735_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1044007.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26520..26906
                     /locus_tag="IW735_000991"
     CDS             26520..26906
                     /locus_tag="IW735_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044008.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27106..27774)
                     /locus_tag="IW735_000992"
     CDS             complement(27106..27774)
                     /locus_tag="IW735_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1044009.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7795 a         5725 c         5146 g         9333 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggatag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt ttgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgcagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc acttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag
    26641 aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta
    26701 tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg
    26761 aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt
    26821 actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg
    26881 aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat
    26941 aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg
    27001 aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag
    27061 ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg
    27121 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    27181 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    27241 ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    27301 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc
    27361 attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat
    27421 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    27481 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    27541 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    27601 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    27661 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    27721 taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc
    27781 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    27841 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    27901 aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat
    27961 ttttacacaa ccgttacggt tgttttacga aattggacg
//
LOCUS       DACEMS010000026        27868 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEMS010000026 DACEMS010000000
VERSION     DACEMS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.026"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(203..3481)
                     /locus_tag="IW735_000993"
     CDS             complement(203..3481)
                     /locus_tag="IW735_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1044010.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3752..4297)
                     /locus_tag="IW735_000994"
     CDS             complement(3752..4297)
                     /locus_tag="IW735_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1044011.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4309..5001)
                     /locus_tag="IW735_000995"
     CDS             complement(4309..5001)
                     /locus_tag="IW735_000995"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1044012.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(5020..5304)
                     /locus_tag="IW735_000996"
     CDS             complement(5020..5304)
                     /locus_tag="IW735_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044013.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5317..5880)
                     /locus_tag="IW735_000997"
     CDS             complement(5317..5880)
                     /locus_tag="IW735_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1044014.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6108..6770
                     /locus_tag="IW735_000998"
     CDS             6108..6770
                     /locus_tag="IW735_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1044015.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            6894..8090
                     /locus_tag="IW735_000999"
     CDS             6894..8090
                     /locus_tag="IW735_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1044016.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8191..9567)
                     /locus_tag="IW735_001000"
     CDS             complement(8191..9567)
                     /locus_tag="IW735_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1044017.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9604..10470)
                     /locus_tag="IW735_001001"
     CDS             complement(9604..10470)
                     /locus_tag="IW735_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1044018.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10559..11584)
                     /locus_tag="IW735_001002"
     CDS             complement(10559..11584)
                     /locus_tag="IW735_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1044019.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11602..12276)
                     /locus_tag="IW735_001003"
     CDS             complement(11602..12276)
                     /locus_tag="IW735_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1044020.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12460..13353)
                     /locus_tag="IW735_001004"
     CDS             complement(12460..13353)
                     /locus_tag="IW735_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1044021.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13427..15124)
                     /locus_tag="IW735_001005"
     CDS             complement(13427..15124)
                     /locus_tag="IW735_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1044022.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(15117..16430)
                     /locus_tag="IW735_001006"
     CDS             complement(15117..16430)
                     /locus_tag="IW735_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1044023.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(16430..19948)
                     /locus_tag="IW735_001007"
     CDS             complement(16430..19948)
                     /locus_tag="IW735_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1044024.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAISDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(20241..20699)
                     /locus_tag="IW735_001008"
     CDS             complement(20241..20699)
                     /locus_tag="IW735_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1044025.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(20997..21515)
                     /locus_tag="IW735_001009"
     CDS             complement(20997..21515)
                     /locus_tag="IW735_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1044026.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(21517..22134)
                     /locus_tag="IW735_001010"
     CDS             complement(21517..22134)
                     /locus_tag="IW735_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1044027.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(22131..22985)
                     /locus_tag="IW735_001011"
     CDS             complement(22131..22985)
                     /locus_tag="IW735_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1044028.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(22982..23893)
                     /locus_tag="IW735_001012"
     CDS             complement(22982..23893)
                     /locus_tag="IW735_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1044029.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(23934..25268)
                     /locus_tag="IW735_001013"
     CDS             complement(23934..25268)
                     /locus_tag="IW735_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1044030.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            25476..26306
                     /locus_tag="IW735_001014"
     CDS             25476..26306
                     /locus_tag="IW735_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1044031.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(26550..26799)
                     /locus_tag="IW735_001015"
                     /pseudo
     CDS             complement(26550..26799)
                     /locus_tag="IW735_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26990..27184)
                     /locus_tag="IW735_001016"
     CDS             complement(26990..27184)
                     /locus_tag="IW735_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044032.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         8102 a         6041 c         4827 g         8898 t
ORIGIN      
        1 ggtcagctga ttgaacaccg tttagtggag agattgtgaa aaagctgtcc tgcatcaagt
       61 aataagaaca gccaatcaaa agcagcaagg agaacctcat ctataaaaag atagaaggaa
      121 gtgagacatc tattggtctc atttccttct atttttactt tcctcagtca attgttttac
      181 atcataaaag gagaaattcc acttattttt ttcgctgttt catgacataa acaactacac
      241 cgattaacaa aattcctcca gctaatgcta gcaacgggtt attaaattct cccgtttttg
      301 ggagtccttg agtgccattt ttttcacttt cttttgctgt cctatccgga tgagaaggtt
      361 ttccatttgg ttttccattt ggtgattcat ttcctggatt ttctctttct ggtttgtcat
      421 ttcccggggg cacatcggca gtttctcgca cagtgatcgc tttttgctgg gtaacagatt
      481 ctgtatgagc agaccatttt tccccatcaa atatttccga acgataagtg acttctagcg
      541 tatattctcc ttttgaaggt gctgaaaaaa tagcggtatg attagaaaat tgagcacttg
      601 actgtgtttc aacgatttgg atcttttctg ggaaataacg catatcttca aagacaggcg
      661 ttccttgatt tggtgtgaaa gttgacacaa aaactgtaaa aggcttcagt tcaatgatat
      721 ttccttctgc attctccgcc acaccatttg cttcttgtat aataatcgga ttattttgtt
      781 catttggttt tgtctgattt ggttcttgta cgattgcata ctctggatat tccgaagacg
      841 gtgaggtttc tccgtcaaaa atagcaccta cagataatgg aagaccatcc actctttcct
      901 tttctgtttg acgattcccc ggtttaatct gcatcagtcc atgaacatgg atacttccat
      961 tttcatagcg cgtaacaggc tgctgacctt ctttcgaaca tgtatttaga gactcgatcc
     1021 aatcttttga tatggtatgt ccaagagtat tttctgattg tttctctcgt tcattccaat
     1081 agtagttcaa cgagtggtag atttgatctt cgttttgatt caatgggcgg atctgctctc
     1141 tcatctcaag attctctgtt cggaaagata agataccaga agcatggtag tctgtgtttc
     1201 ctgctgaaga ggtataaaga gccacattat atgaaccgtt attaaaactt gtactgctat
     1261 tgacgataat atctgttccg ctattggaat cgattccttt cgcaagattt tcgaaagaca
     1321 aactattgat caattcatga gggcccgtta aagaagaacc gcccattttg aatccattac
     1381 cgttaccttc tccctcaatt cctggtaccc agccgtttcg ataagcaact gagtttttga
     1441 tgattacttt tccgatcgaa cctgtttcat ttttagcaaa aaggtcccaa ccgtcatctg
     1501 cattatgata cgcgatacaa ccgtcaaata catttccttc tcctattgtc agtttggccg
     1561 caaatccatc ggcatcttca aatcctggat cggcattttc aaaagatgta cagttcttga
     1621 ctaagttatg cgcgggccat gcttcgaaag gatcatttga gctaccgctg atttggattc
     1681 ctgtatttcc attccgataa gcatgaatct cttcgatcaa attatgattt ccagaaatct
     1741 gtaatccttt ttgcatagct gcactatttg taatgtcaaa ccctcgtaaa ccccagtagt
     1801 gactgaacag tgtcatccca tttccagttc ctttaaaatc aagtacagga cgcttgtttt
     1861 ctggattttt ctctgcaatc aactgaatcg ggttttctct tgtcccgtta acgccttttg
     1921 gaatcgtcaa tcctctttcg aaggaataag ttccaggcgc catgacgatt gtctgcccag
     1981 catatgcgta tctcaatgct tgtgtcagag ataatggatt ttgtctgctt ccgtttcctg
     2041 catctgaccc ttctggtgtt acataaactg tcgaatattt aggtttacgg tagtccactg
     2101 tatgaagaat ctcttttgtt tcataactag tcatctctac gtattcttct ggcggatagt
     2161 ttttatctgg cgtaaaactg aaagtaaatt cgttcgttcc ttccatcagt tttgtctgat
     2221 aatcaaattc ccgattcgca gtgatagcca agttttttgc gttcttaagc acctctcctg
     2281 ttgctttatt tttaattgtc aataatccgt cagcatttgc tctaaaggac atagtatgat
     2341 cttggtttcc tgaatgatta ctagaggtca cagtgtaaat cggatcaatc aagcgagtag
     2401 gtctttcttg cccaggagga tctgtcgcag gatctgagta agcaacttcg attccttcta
     2461 ccttgatcgt catattcctt gctgcagcaa atcctgcata aataaccgat tcatctgaag
     2521 cgtataattt ttcccagtca tacatgatgt cagaagccat ttcttctcct gtttcaggat
     2581 cgtaataata tgcctctaaa ccagtattcg tttttttcat ctctacgtcc agtttagtaa
     2641 tcgagttcgt ggccggaaac agattcccat gaccgtttcc gaatatatta taagaacctg
     2701 tttctttttc caagaaccct gctgaagtct ctaaaggtgt tgtttccact gagacactcc
     2761 caggagctgg cgcagcttgc ggatcttcag atgatattcc tgtgattgcc cgtgtcccca
     2821 atccaagtcg catcgtatat ttgctgccgc tcgtgttgac cacttcttgt ctatccgagt
     2881 cccagacata ttcgatccga ctgcctagta tggcaaatga atttgaataa aatgcctgcc
     2941 caaacaaatg attttttggt actgcgtctc ttaccattaa agcaaatcct tcttgggcat
     3001 tcgtataggt ccaagattct atttcgatcg tcgcccgcat cctaaagttg atgtctttgg
     3061 ggattgcttg ataataatag gtcattccat ctgatccgct tgactggaat tttccgccgt
     3121 tattcgtgga agagagccgc acgccgtctt ctaatggttc aatggcatac ctactagcac
     3181 ttgtgctttc tccgaaaaca acccctttcc actcttcatt tgcttcttca gattgctttt
     3241 ttgtcttttc attttctgat tctttcatca taggatcatt tccttcttcc gtatccatat
     3301 cttcactagt tcttggtatt tcctccattg ttgacatccc ttcaatgttc acgtgtgcat
     3361 tttcttcgtt cacagactgt tgttcgttag cagcaacttc aacgacagaa attaaaaccg
     3421 gggtactgag cataaggaga ttcatcatta gcgcactgta ttttatacgt ttttttaaca
     3481 agtgtattcc tcccgtaaca aattagaaag cgtttactta aagtattacc taatttattt
     3541 ttactgtcaa taaaaaacaa aattataata agtgtatttt ataaaaagaa aaaatataac
     3601 ttaagttgtt gataataaag agatttatta acgtaaaaag aataagtttt tttaaaataa
     3661 cgtataaaaa agagcataaa acaaagtcgt ttgttttatg ctcttctttt attattttgt
     3721 gctttttatt ttatcaaatc actttgtttt tttataaaat ttctgcgcct agtgtggact
     3781 caaaatgtct caatgcccat tcatgtccaa cgggatcgaa acttgccacg gcatctttga
     3841 aaatatggag tttgtatcct agattatacg catcgactgc tgtatgcaac acacagatat
     3901 ctgtacatac gccggtcaaa taaatatctg tgatctggcg ttctcgcaaa cggatgtcta
     3961 gatctgttcc actaaaagcg gaataatgcc gtttgtctat ccaataaaca ttttcctctt
     4021 gctcatagtc ttggtataaa ctttttaaag agccgtacaa ttcccgtcca cccgttccta
     4081 tcacattatg cggaggaaat aagcggttct ccggatgaaa ggaatctttt ggatcatgcg
     4141 catcaatggc aaaaacgaca aaatcctttt gatcaataaa cttctttgta tgagcagtca
     4201 gctttttttc aattacttgt cctgctgtcc cagtagtcag ctttccatcc tctgcaacaa
     4261 aatcatatgt gtagtcaatc gagatcaatg cgttcatctt agtcctcctt atacgagatg
     4321 tttgacaaat tcgatcacca tttctgctga tcgtttgcct gcgtcttcga taaattcgtc
     4381 aaagctttgt gtcgccgagt gatctgctgt gtcgctcatt gcacgtacga tcaaaaatgg
     4441 gatattgaac tgacgagctg tttgtccaac agcggctcct tccatttcgc aagctaatgc
     4501 ttcagggaaa ttcgttaaga tttctttgat tttatctggc gagtcgacaa atgtatcacc
     4561 tgtaacgatc aatccttctt ttgcgttcaa attcgttgct tttgctgctt taaccatttc
     4621 actgcgcaaa tatgtgcttg cttcataata tagaggcatt cctggtaatt gtcctgggtt
     4681 gtagccaaat cctgttgcat ctgcatcaaa ataagctact ttgtctgata taacgatgtc
     4741 acctacttgt aagccttcac cgattccgcc agctgaacct gtattgatca ccatgttcac
     4801 gccgtattgt tggatcaaca gacttgtggt aatagatgct aagacttttc ctataccgga
     4861 acgtacgacg atcacttcgt ggttacctaa tgaacctgaa ataaataaag cgcctgcgcg
     4921 ttcccatgat aatggttcgc ttaggttctc tctcaaaatt tttacttctt cttccattgc
     4981 gccgataata ccgattttca tctttttaat tccttctttc tatataaatg cgattgctaa
     5041 aaaaaccaca cataacatta ggataacaat aataagagat ttcatcaaaa aagagttcca
     5101 tttggtcgtc ttttcccgtt cgctgatcct tgttttcgtg attggtttat tctttttgct
     5161 ttctttacga taaaagctgg caatcttttt ttcttcttgc tgataagctg tttctgcttt
     5221 tcgctgtttt tttaatgact cgcttgcttg tgcttgttgt cgcttgcgaa gttcgcttcg
     5281 cgtaataagc ggtcctttgg ccataagccg atccctctac tttcctttgg tcaatagttc
     5341 ccagtattga atcgcataaa tcgtctttgc atcacaaatc aactgatctt tcatcgcttg
     5401 ttgcgcctct tcaagagtta gatgatacaa ctccagtact tcgtcttcat cttgtgctaa
     5461 aggattctcg accttctcta ccccttgtgc atgataaata tgaaggactt catttgcaaa
     5521 acctggtgaa agatacatcg aagtaagatg gctcagcgac ttcgcacgat agccagtttc
     5581 ttcttctaat tctctagctg ctgtcatttc gggattttgt ccttctcctg gatcgatttt
     5641 tccagctgga atctccaaaa tcactttttc taaaggtttt cgaaattgtt tgacaagaag
     5701 cagacgatct tgttcgtcaa aagcaatgat cccgacaccg ccaggatgaa ataccagttc
     5761 tctcttcgaa gttcctccat ctggcaaacg aacttcgtct acagcaacat caataatttt
     5821 tccgctatag atttctttgc gactgattgt tttttcttca aattgctcat tgttcagcat
     5881 atcttctcct gctttctttg taatcataac tgcttttttt tcatttttca atttttctca
     5941 aaaactcctt attagtttac catagataaa ccttagattt tctctaaata tagaaaatcc
     6001 ttctttagtc cgatactctt tatagtagtc cgtatgctaa aataagtatg taatcgaata
     6061 acgaaaagaa ttcaatactc aacgataaat gacatggagg gtttattatg ataaaacgaa
     6121 attggccgtg gcttctagca ttggtcttgc tatttctctt tgtccgaaac aatagttttt
     6181 cttttgttct aggactttta ttgataggtg tagttttagt tttgtgggga ctctttggtt
     6241 cgcgcaggaa aaagggaaaa caagaagaaa ttcctgcact gactgacgaa cttgaaactc
     6301 actatgaaaa aagcggtatg acagcgaatg aaatttcatt ttttcgacaa acaatgaatc
     6361 aaacaaaaaa tgagatcaat caattgcaaa tgaatatgca gcagacagcc aaacttaaag
     6421 caatcgatct ccgccatgat ccagtcaaag cagcgaaagc tttattcaaa gaattggtga
     6481 aagaaccaaa ccgactgcat gaagcaagtc agtttcttta tacgcatctg ccgaatctag
     6541 ttgatttgac aaataagtat aatgagatca atgaccatga gatcaaaaac aaacagacct
     6601 atgaaaaact agaagaaagc gcgcagatca tcgaccagct gtcagcttta atcgcacaag
     6661 attatcaaaa attcgttgct gaggatctag atgatatcga cgtagagata tctgttgcga
     6721 aacagagctt aaagcgtgat aataaatatg aaccagaaca aaaggattaa ctcttttgtt
     6781 cataaaagaa gactcggaac attcagatga tgtctggctg tttcgagtac tttttctaaa
     6841 gtagagacat tcatgtaaga atcgagaaca gattcaacaa ggaggaattt tttatggatc
     6901 ataaaccgac tgaaacaaaa gatgttacac cagttaacga tacacttgaa gatttattga
     6961 acaacccgtt ttcaactcca acagatacgc tgaccacttc acaacaagcg gagattgatc
     7021 agctccaaga gcaacagaca gctgctcgtt taattgacaa actaccggcg gaacgccaag
     7081 aacaagctaa acagctagca gcaaaaatcg atgctaatga tgcacagtct gtcatcagct
     7141 acggatcggc tgcccaagca aaattaagtg aattctcaca atcgatgttg aatcatgtgc
     7201 aagcacaaga tattggtcct gtaggagatt ccttaactga actgatgtat cgtctccaag
     7261 aagccaatcc tgacgaactg cgtgccggag aagggaacat cttccagcgt gtcttcggga
     7321 aggtcaaaca atcgatctat gaagtgacag caaaatatca aaagattggt gcacaaattg
     7381 acaagatttc agtgaaatta gataaagaaa aagacggtct gctaaaagat aatctgatgt
     7441 tagaacagct ttaccagaaa aacaaagact actttgatgc actgaatatt tatatcgctg
     7501 ctggagaatt gaaaatggaa gaacttcaaa caacgatcat tccagaagcg atgaaaagag
     7561 cagaagaaac tggcgatcag atggatgttc agattgccaa tgattacacg caattcctag
     7621 atcgtctgga taaacgtacg catgatcttc gcttagctag acagatcacg atccagcaag
     7681 caccacaaat ccgtttgatc caaaacacaa atcaggcctt ggctgaaaag atccaagcat
     7741 ctatcgctac agccattccg ctgtggaaaa accaagtcgt gatcgcattg accttgcttc
     7801 gtcaaaaaga tgcagtaact gctcaacgcc aagtctctga aacaacaaac gacttattga
     7861 agaaaaattc agaaatgctg aaaatctcag ccatcgaaac agcgaaagaa aatgaacgtg
     7921 gaatcgtgga tatcgagacc cttcaaacca ctcaaaatga tttgatcgaa acaatccaag
     7981 agacattgcg tatccaaaaa gaaggaaaag aaaaacgtcg tcatgcagaa gtagagcttg
     8041 gacacatgga agaagatctg aaacaaaaac tgttggattt aacccaatag atcacctttc
     8101 gcttgttact aaaacaaacg cccaacaaca gtttattaca actgttgatg ggcgtttttc
     8161 ttatctttct tctaacttct ccattggagt ctatttctca ttcatcttgc tgtgtccata
     8221 gccatagcca aaataaatga tcagtccaat aaccattacg ataccgaaca cgatccatgt
     8281 cacagtttgc agacgaagca tcaatacaat acaagtcaag atggagacga ttggcaatag
     8341 cggaacgaat ggcactttga attctccagc ttttggtttg ccaaacattt ttctcaactt
     8401 cagtaagcct agactaacaa gcatcaaata ggcgagcgcc atgatattcg ttagttccgc
     8461 taatacattc aatgggaaaa atccggcaaa gaatacagct gccacaccag cggcataagt
     8521 cgcttttttc ggtgtacgat gcttttgatc gatttctgtc ataaacttag gaagtaagcc
     8581 atccttgctg acagcaaaca gtaaacgagc aagtgaatac atcatcgaga tcgttaccgt
     8641 cagtagcgtt aaaattgccc ctacagcgat caccgcgccc actgttccat gtcctacata
     8701 cctcattgca aaagccactg ggtcattgac tcctagttca gtatacggaa cgattcctgt
     8761 caagatcagc gtcacgatga tgtaaaggac agtagcaatc agtatcgaac caatgattcc
     8821 tcttggcaca tctttttgcg ggtttttgac ttcctcggct gccattgcaa cggcatcaaa
     8881 tcccaagaaa gcaaaaaaca caagagctgc accatctagc acaccggaaa aaccaaaagg
     8941 catgaatggc tgccagttat caggtttaac gaaaaatatt cctacaatga taaacaacgc
     9001 gatgatacca aatttgacat ataccatggc attattcaaa cgtaatgctt tcttggcttc
     9061 aagcgacacg atgtaagtga cgaatatcac tacgagcacc gcaatcaagt caatgtatgt
     9121 accattttcc ggattgtacc cgccagttaa tgcttgtgga agttcggccc ctaaactttt
     9181 caaaaaacct tgcatatagc cagaccatcc ggaagcaaca gaggaaaccg ctagtaaaaa
     9241 ttccccgatc aacaaccacc ctgtcagcca ggcagcaaat tcaccaaata caacatacag
     9301 ataggcatat ggcccaccaa tcactggcac tcgcgaagca aattctgcga aacttagtcc
     9361 agacaaaatg acaacgattg cagctaagac aaatgaaagt gacagtgctg gaccagcatt
     9421 ttgattggcc gcaacccctg tcaccacaaa tattcccgta ccgatgatcg caccaattcc
     9481 aagcatgatc aaatcagacg tttttagttc ttttttcatc tcactcgtat gattgagatt
     9541 gatctctttc ttacgaaaca aatccattga agttcctccc cattttaggt tggaaaacag
     9601 cgattaaact gttttcatat accctaaaag gataccacct aaaatgacta aaaggcaacc
     9661 aacaataaca taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc
     9721 taaagtagag ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga
     9781 tttgactgtt agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt
     9841 tttccacaca aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat
     9901 cattccgata ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat
     9961 gatggtatat acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa
    10021 atcaagcatt ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata
    10081 cgcaccgctt acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt
    10141 ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga
    10201 caatggtagc cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc
    10261 aacactccaa gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg
    10321 actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt
    10381 ttgttgattg gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa
    10441 catagggact aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag
    10501 aaacttatca gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct
    10561 aacgtttttc attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc
    10621 cttcagttga ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat
    10681 aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct
    10741 tatcaaagat ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa
    10801 ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat
    10861 atttgatggc attactaagc aattggttga agataaaagc gatccattta gcatcggtca
    10921 aaacagtatg atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg
    10981 cctgactgcg tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt
    11041 cccgcgagaa actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt
    11101 ttgataattc attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac
    11161 gaactagcaa tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat
    11221 aatctttttg gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga
    11281 tcagctgatt gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat
    11341 aattttgcaa aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc
    11401 tcaagtaacc acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag
    11461 caattgtgct caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg
    11521 caataaaaaa cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat
    11581 tcatcttaac cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat
    11641 atatccttgg atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa
    11701 tgtattgtca tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact
    11761 taagatcttt ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa
    11821 atcaatgatc tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt
    11881 gtggctcatc atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt
    11941 gatttttgca atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat
    12001 gttcatagcc atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac
    12061 tttcgatact tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat
    12121 atctagtaat acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc
    12181 ggtcgttcca aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat
    12241 gatctcttcg tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct
    12301 tgctactaca atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat
    12361 tttagcaaac gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag
    12421 cagcttaaat ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg
    12481 ttgtttttgt cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa
    12541 tatacctaga aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt
    12601 tgaagagact tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt
    12661 cacggtgtaa ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa
    12721 tgtatttgtt tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg
    12781 accatacact tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag
    12841 ttttttctca gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt
    12901 tggaaaagag gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc
    12961 atcaaataaa ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg
    13021 ttcgtaatat tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt
    13081 gacttgtccc attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg
    13141 cactgctaat tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt
    13201 ctcgatcctt ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa
    13261 aactgcagct ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg
    13321 ggtttgaggt ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac
    13381 tgcagcaacc tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact
    13441 tcattttaga tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta
    13501 agaatgccaa gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta
    13561 ctctttctgg ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat
    13621 aagagatgat aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg
    13681 ccatggtctc tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat
    13741 ttccatctgc gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc
    13801 ctgattcata aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt
    13861 cttcattcca caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt
    13921 tttcaaactt tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat
    13981 caaattcatc atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt
    14041 ctagttctgt ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg
    14101 ctaatctacc taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga
    14161 caaacccgtt gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc
    14221 cggctcgcac ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga
    14281 ttttgtaatt tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa
    14341 taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg
    14401 tttcttgttc tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat
    14461 tcatctgttt atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt
    14521 atgtgtcttt ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg
    14581 gcatcataac aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta
    14641 agaaaagata gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga
    14701 ataccgcttc tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta
    14761 aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac
    14821 cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta
    14881 cgtcaaacaa atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca
    14941 atccgctatt cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg
    15001 tcacttcact tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa
    15061 tccctattcc cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat
    15121 tcattttttt ctccccgttc tttttttatt aataaaacag cacctaacaa taaaagaatt
    15181 cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga ccattttcct
    15241 gattgacctg gattgcttgg accactcggc tctttcggtt tgtccgttgt ttcttttacg
    15301 gtctcgttat aaacacgtgg tgttgcggat tcataggcag agactggaac tttcccggat
    15361 tctaattcag ccatctcctg tccgttaact actacatatt gtggttggcc ttttgcatcc
    15421 acccaaaatt cagggatgat gacctcgatt ctcttacttg cttcatccat ctcgtaacct
    15481 tccacacctt ttaattcttc aaaataatag gtgccagaag gcaagaaacg ttgtcctgtc
    15541 gtaacaaggc cctctttatc cgaaatgaat ttgcttacat ttttgtcatt caaaggatca
    15601 ttgtctgctg gtttgatcca ttgatttttc aagtcattag caggagccat atctaaataa
    15661 tatttccccc cttctatcat ctgatataga gcaaaaacag caccttctaa tggttttcct
    15721 ttttcagttg ttcctctttg acgtccatac ttgaagaagt atggatctct tagataaccg
    15781 acatttttag gatagatatg aatctcttgc agttcctcgg ttgattctgt cgggtgataa
    15841 attggcaaaa tagcagcaat tgggatagct gtctgatcga tatcaacatc caatccaact
    15901 tctggatcaa tcgattcttc gaaaatcaaa taaactgcat cccgatcttg gtttttctta
    15961 ggtgctttga tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt cccagttgtt
    16021 accgaagtcc ctatttggac aagcctttct tctgtgatta gctgacggat attgtttctg
    16081 ttcgtttcag cgatttgctg gacgaattct ttaggagtca tgttttcagc agcttttttt
    16141 tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag tggcaccgtc
    16201 tgtgaaatga tatctgctgt ttcccctgct tcctcagaga tagccaagcc atcattttca
    16261 tggtattcaa attgatcgtt ataatcgata tctcgaatca tccgtttatg caacacgaat
    16321 tccacgacat cttcccctga ttcttctgcc gacatcgcat gtggaagaag gactgctgca
    16381 agcggtagta aaacaagtaa ttgaagcatc aaacacaaca gctttttcat taccttgctc
    16441 cctccttttt agattgtctg acaaacaaac cataggctgc tcctgcaagc gccatgaaga
    16501 atgcagcaag caggatatag aataatcgtc cggatcctcc tgtagcaggc aacggatttt
    16561 caggatcgtt cccgacacta agctgtaatc tgattcgatt tgtctccaaa agttcatatt
    16621 ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt tggataatac gaaatctcaa
    16681 cttttagtgt acccgcttct tttcctggaa ctacccgatc attttctatg tcaggttctt
    16741 ctcgataact aatttctaaa ataaagtaac ctggaagttt tctatagctg tcaggtgact
    16801 tcacttcctt gatccgatag accccttcca acaagtctga agcattgaat agggaagaat
    16861 cgtttggatc tggtgccaag cctccctcga tgatttcata ctctcctgca ctattttgtt
    16921 tctcaattga aaattctgcg ccttcaagac gtcggctatt catactgtca attttcaaaa
    16981 gttccaaatc gattggcgct agttcatctt ctaaggttac ttgtagtaca ccatcgatga
    17041 tttcatagcc ttccggcaag ggagcagttt cgtcttcgat gaccgtatct tcaatgataa
    17101 acttaccttc ttcattcagt gcaaactgga acacttgatc agatagccga tacccatttg
    17161 gtgcgacaga ttccgagacc tcatattcac caatagacag atgcagaggt tggtcgtaat
    17221 ttaaatcgac atacttttct actcctacgg cacgagaata gaagtatccg atcccaggca
    17281 aacgtttcga ttcttctggt tctaacggat taccagtgta tgctcgatac agcgggaagg
    17341 aagaatcccc cactttcctg atatcgaaac ctgcaccatc taatgtatgg ccttcatggt
    17401 ctaattttct aactcggatc tcccgataat caaacgtatt gtaagcagtg aatgctaccc
    17461 ataaacgatc ctcttcatct gttgtatacg tgagatattg ccaattttct tcacctgttt
    17521 gcttgagttg gatagtccct atagcattat cgtaaagtcg caagatccat gtgtcatttt
    17581 catcaggttc tttgaaacct tctgggttct ggctctcaat gattttgtat tctgcatagt
    17641 tggtagtttc ttcactatcc ttataaacaa atgaatctgg ctctccgatt ggatgaataa
    17701 ctttctcacc atccacgatt acatatttcc cgtcttcatc tactgaatag aacgtgagtc
    17761 tccctttctc ttcctctact tcgttcacac cagaaagccc tttcgctact ggatatccga
    17821 tttccagtcc gttaccatct gtttcataaa gttcaaactc tgcgccattg attggcatat
    17881 tactgccttc aattagttta ttgatattca tgtggattgg cttgaattca tttcgcattt
    17941 ccagtttgac atacaactca tcatactctg tgccttctat gttaggctct tgtacaacag
    18001 aaagcttgtg atattctcct tcataatagg aattcggatc tttaccttcc ggtccgccga
    18061 ctctcgaacc agatgtatat tcatctacaa gctcgtagtg acctaacatc tcatcttcgt
    18121 tggctgcata ttttttgatt tggaattgat acgtctcatc tactaactga taacgtggtg
    18181 gtccagcgat ctctctgatc caaaatatgt cgctgattgc tccagtttca tctcctaaaa
    18241 tcggcatctt cgtttcaaag atcgctattc cgtccgtggt agttttactt gtttcgattg
    18301 gttcggcttc cccactctct ggattttggt catataatgc aaattcgact ccagccaacg
    18361 gctcgccatt ttgacccttc ttgctgactt ggaaattacg gcctttatgt tcgtaacgaa
    18421 tggaagaaga tgggaaatac atccgtccag tagtgatatc attctcataa tcctccaggt
    18481 tctgaatata ggccatattg ttcactgtgc aaaaatttcc gtcttgagct cccgcatcaa
    18541 gttttacttg gtacttgatc ctgaccattt ccccttcacc taaagaaatc ggagaaaatt
    18601 tataagcttg gttttcccgt ccgacatacg ttgtcggagt cgccactact tctggtgggg
    18661 cttgtccatg acgttcttca tcccatattt ctggctcttg gtacttcatg ccgttttctc
    18721 ctggatctag atggtaaaac tgaatatctg ccgatctgcc agctccaaat ggtgagacat
    18781 taaaaccagt ttcatcataa agtgtggctt gctggatcgt tctcttgaag gtagaagaga
    18841 tatcctccaa ggctgtaaca atgtcattct tgttgttcgc attgtaatat cgcggtgtta
    18901 cgccatcttc ttctaaatca gcaatatttt tcaatacatt tcgtccaata gcatctagtc
    18961 gttgtctccc gctaacgttt ccatcgattc ctaaaccaat ggagaagata cttggcgggt
    19021 tctcttgata cttgtttttc agccaatgat gataagcaac tgtgttgatc gcagatgact
    19081 tttcttgcga agtaggactg ccattcgtat tgttattaga agaaccatca ccaacccagt
    19141 gagtcgcttt attatctcct tcttttacct ctccataacg atagtgtaca tttacttcat
    19201 cttctggacg gttaaagtca gatgcatagg taactggatg agtaaaccct gcattagaag
    19261 tattcccaga aaactcctca aaatttctga acaaaacgtc attatcttca gcaatcttat
    19321 cttccatcac agtccagttg ctccaagctc cattagtgct cgtacgataa cttgactgaa
    19381 tcggtgcata actgaaggtc ggtgtcccat ctcccacaac gattaggatt ttttctggat
    19441 ttctattttc tccgccattg tctttataca atgtttcata gccattttta agccctagtg
    19501 taagcggagt tcctccgatt ggtgtacgat ttaatgctgt attctcactt cccgtcaaac
    19561 gattgatatc atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg ttattgatag
    19621 ctgtactatt atggtaaaaa ttcaccatac cgattcgaat atccagactc gggtcagaga
    19681 gcagatcttt actcatttcg tttactgcct caatcaacgc agcattttta gtttgactat
    19741 tggtcccagc ggttaattca ctcatactcg ctgacttatc tagaacaaaa acgatatcga
    19801 ttggactgat ttcctttttc tcgcccccga tgacatctag atagacattg taaagggttg
    19861 ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg cagaacaggt tctacccatt
    19921 ttttgatgta cgccccgtta taatccaagt aactgttttc aaaattccgt tcatttcccc
    19981 aaagctgaac ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt gatccattat
    20041 tagtagcata gtcgtaattt cgccagttcg ctcccccgtt tgtataatca ctatcatcga
    20101 tcaagttctc tccgtcatat ggcgagcctt tgtctccttg atagcgttca ttgctttgtg
    20161 tccaatttcc taacactttt gtatagtcat tcgttgtaaa cttcatctat tctttcatca
    20221 accaccactt gaagaggtag ttacccttcg ttcacatcgg ggatcttttc tgtttcaaaa
    20281 atcaatgtgc cggtttctgc ttcttttgaa taaacttttc cgacatacca ttcttgacca
    20341 tcgatcgtct cgacttttag gtcttctcca tcttcatcat aggaggatag acctgttcca
    20401 cgaacgtttt ttacggtacc aatcccggcg gctgccgcat cgattgctaa acgtattcta
    20461 ccttcattgg cactttcctt tttttcaaaa gccagatgcc attcaagggt ttcttctttt
    20521 tcaatatact tcccttcaat ttttgcatag tcctcatcta agaatacctc atcctccttt
    20581 gtttctgttg actcagcagc tacatccagg gtcgtcatat gatagactga ctggaaatat
    20641 ggtccaaata gcaaactgac agttactaac tgcaaaagct tcaattttaa ccctttcaca
    20701 tccttatact cctttcaaaa atatttgtct tctttcacaa caatcaacaa aacaatgccc
    20761 atctaattat aatttaattt aattttaatt ttaaatcaaa tgatatcctt tttagttaaa
    20821 aaacaccaat tatacaacat ttattattaa aattaaatta taaaatttta taacgttctc
    20881 atttaacaaa acttaagtta ttaagacaaa tgaataaaca actacgcttt cctcgaaaca
    20941 aaaaaataaa gcacaaatgc gcatttacgc actcatgctt tattcgtaca attaattcag
    21001 ataacggcag cgcataggat gccaataaca cttcctaaaa tagctcctcc taaaatgtca
    21061 gtaggataat gtaaaagtaa aaaaatcctc gaataagcga tcaaacaagc aaataaaaag
    21121 gctaatattc ctgcaactgg acttaattcg aacaacgcag ctactgataa aaaggctagc
    21181 gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac ttgtgtaaag gacaagccga
    21241 cgattttttt cataaggtct tttccgtctt gtcgtttttt tgataaaaag caaaccaaca
    21301 aaaaaataga tcaaactgcc gataagaatg gctagaccat attctctagt atcaggtatc
    21361 aagactaaaa cagcagcaag gagcaaccaa ataaaaccac cattagagat attactgata
    21421 aatttcaaaa aattgatttt tcgactatcc gttttccgtt gcttggcgaa cttgttgata
    21481 atactatcat cgattttagc aatcaaacgc aacatgttat tcctcctttt taagaacctt
    21541 ttcttgcgta cgatggataa aaagcaggac ttgaaaataa aacaagaaac tgaaacctaa
    21601 taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca agaaggatca acgtgcctgc
    21661 caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag aaaaatgcag taatccaaga
    21721 agcttctttt tgttccagtt gaagcaagat tttgtaacta aagagaagcg cgcttaatag
    21781 aagcaaaaac aacgtagtat acatcaatgt atagccaatt cccccacccc aatagcggaa
    21841 aatctgatag tcgatcaata gaagaagaaa ataaacactt gctaaactat cttgctttcc
    21901 ataaagagag aagttctcac gaaacttttt ccagaatagt gaaccgagct ttttctcttc
    21961 attttttttg taatgataaa tcatatacag aactgttcga cttgcagttc ctaatgtgaa
    22021 aacaggcaag acgcacagga accatagtag ctgtaaatgg ataagacgtg gaatccattc
    22081 aatgatttct aagaaataat cattggcttt tttaccaatc gatcgattca tcatcctttt
    22141 gaacctcctg tcaaatccat cccttcaatg aaataacgtt gtgcaaaaaa gaacagcaaa
    22201 atcgtcggga tcaagacaag tgttgctcct gccatcagat aattccattg tgtcgttgct
    22261 tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt tttcttgatc ttggatatat
    22321 aacagaggac ctaagaaatc attccaagct gcgttgaagg aattgatcgc aatggtaatc
    22381 aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa tatacaaatg attcgctccg
    22441 tcgatttcag cagcttcgat caattcatta ttgatcgata agtaaaattg acgcatcaga
    22501 aatatattga acgcattgcc gaagaacgat ggaacgatca ggggcaaata agtacctacc
    22561 cagccgatct tggagaataa tacatattga gggatcatcg ttacaaaccc cgggatcatc
    22621 atcgtagaaa gaaccaaagc aaacatcagc tttttcccag gaaaatcaag cttcgcaaat
    22681 ccataagcaa taaaagaatt tgaaaggaca ttaccgatca ctactagcac agtgatgaat
    22741 aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa tcgtatccag atagttttcc
    22801 catacgattt tttctgggaa aaaggtcgga ggataacgaa cgatttcttc cattggtttc
    22861 aatgaagtaa acaccatcca aagcaataga atgatcaaaa tgattcctat tactgtcatg
    22921 aggacataac ttagtatttt ttttgtttgc cgattcacac tggtcttttt gaaataatcc
    22981 atcatttgcc gtcacctcca gaataataga cccacttagg tgcaagcttc aaattgacaa
    23041 acgtcagaac caacacgatc agaaacagga tcattgacat agccatcgca taacccatat
    23101 caaaagctac gaacgccttg ttccacatat gcagattata aaaaagcaaa gaatttgcag
    23161 gtccgcctgt tccatttttc gtcatgacat aagcttcttg aaaaatttgg aaagaaccga
    23221 tcgtagaggt gatcacatcg taaaaaataa taggagtgat taatggcaaa gtaatacttt
    23281 tgaactttcc ccaaacgcct gctccatcga tttctgctga ttcatataaa gatctaggaa
    23341 cattttgtaa tgtagctaag taaagtagca ttgctcctcc agaactccat attttcatca
    23401 aaatcaacgc aggtttcgtc cattgtggat caaacagcca agctggtcca gggatatgga
    23461 accagcccag aaatgtgttg atcaatcctg cagatggaga aagcagctgc atccataaga
    23521 tgtagactgc tactcctgat aagactgccg gtaaatagaa agctgtacgg aatacactga
    23581 ttcctttgat tttttgattg agtactgtgg ctaaaaacac actgaaaatc gtcgtcagcg
    23641 gtacattgaa gatgacgaaa taaacggtgt tgtataacga tttccaaaat aaatcatcgt
    23701 gaacgaacat tcttttgaaa tttgctagac caatgaaatt catcttggat gtcaaattat
    23761 aatcggtgaa actaccaata actgaaaaaa gcataggccc tagcataaat accaagaacc
    23821 cgataataaa aggcgagata aataaatagg cccatcttgc ttcttttttc tttcggatag
    23881 ataaacgctt caaattttct tgcctcctct gtacctagac agtcaaaaat ggattattga
    23941 ttttgcttga ctaagttttc tactgctttt tgtgcatctg ccaatccttc ttttggtgat
    24001 tttgttccta aaagaatctg gtcaatctgt tcattcacta atgtcgtgta atccggagca
    24061 tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt cgtccgccat ttgatagatc
    24121 atttttcctt tatccgttag gttgtcatta ttcactaaat tctcattggc tgtcttgctc
    24181 gccatgatat caaaactttt ttcgccgaat ttttcttgta cttctggaga agaaagatat
    24241 ttgatgaatt cataagaggc ctcaggatct tttgctccgt atggtacttc caagacgaat
    24301 ccgccacccc acgaccaatg gcctgaacct tcttcttttt ctgggagctg catcacgcca
    24361 aaatcaaaat catccggtgc attttccatc aaactagagt aataattgat attttgggca
    24421 cgcatggcta ctaatccgga aataaatgga tcagatacac cagacccaaa ttcggcttct
    24481 aatttattga tcgtattttt cccataataa tcttgccagt cttggatcca ctccaaggct
    24541 tccactttat ttttagtatc aatcttgact ttttcatctt tgtcaaacca actagtcccg
    24601 ttatctgcgt ttagcgccca tacatcagcc cctaaattcc ataatggata aaaaccgatg
    24661 cgtgtaaatt cttttccttc ttttttatct agtttacgtg ctgtttcttc taattcatcc
    24721 caagttgtcg gtagatcttg ttcactgatt ccagcttctt taaataactt tttattataa
    24781 aaaatgactt gtgtatctgt attgaatggg atcgcataag gatcaccttc atattctact
    24841 gtatcccaaa gctgcggata aaaatcctta gaagtgtctt tttctacata tttggagaga
    24901 ttagttgctt gctgtgcttc tgctctttgg gcaacggaat ttatgtcctg tacaataaca
    24961 tcgggtgcat ttccagctgt tattgcagat aaagatttag tccagatgtc accccatggc
    25021 tgatacttat aatttacatg gatctcgttt tgtgattgat tgaaatcctc aatgatttct
    25081 tcgatagctt cttttcgtgc accggatccc caaaaggacc agaaatcaat attttttctt
    25141 ccatcacttg ttttttcaga agaatttgct gcacttgaac tggcatcgct tgcattactt
    25201 gatccgcatc ctcctaataa aaacaatgct gaacttgcca gcaaccctgt aaacaataat
    25261 tttttcatat tttacctcca aattttaatg taaccgatgc cggttttaat ataacgatat
    25321 cattttgttc tgaaaataac aatttataag gtatgattct aaaaaaatta ttaatgatac
    25381 caatacttta tataattcgc gagcgctttc tttgaatttt ttaatctttc gtagcagaat
    25441 gagaatgtat taaaaataga ttaggaggaa ttttgatgtt tagacaccag aaagaattgc
    25501 agtttgaagc aaaaccagat cgtccgaatc cattaatcgc gaaatatctg caggagctga
    25561 tcggcggcca atatggcgag atgagtgtag ctatgcagta cctgttccaa ggctggagct
    25621 gccgaggaga agaaaaatat aaagatatgc tgatggacat tgcaacagaa gaaatcggcc
    25681 acgtggaaat gttggcgaca atggttgctc gtttattaga gggtgcgcct gttgaagatc
    25741 aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat tatctctgga atgaatccac
    25801 agcacgcgat cgtagctggc ttaggacctc gtcctgctga cagcgtgggc aatccatgga
    25861 gcggtgccta tatcattgcg agtggcaact tgttagcaga ctttagagct aatttaaatg
    25921 ccgaatcaca aggaagactt caagtagcac gtatttacga aatgatcgat gatcgtggcg
    25981 taaaagatat gctgtctgtt ctccttgcaa gagatagcta tcatcaaaat ctatgggaat
    26041 gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt agtcccaagt actttcccgc
    26101 gtgacaaaga acgaaaagac attgcttatg acttctataa cttctcagag ggagaccaaa
    26161 gtagtgaagg ttcttgggct agtggcaaag cactggatgg cgaaggcgaa tatcaatggt
    26221 tgaaagaacc taagatcgaa ggcgctgcac caaaattaga accagtaaca ccaaaaatgt
    26281 atgggacacc tccattcaag aaataaatat ctacccgtag aaacacccgt tttttgttcc
    26341 acacgctttg tgagcaaaac tgactaaagt cattaatcat aaccaccctt caaaagggtg
    26401 gttttctcta gagctataag ctcaaatcac cggccagcgc ctaaagacgc tggctttcat
    26461 attttatgtt caagccatat tgcctttgcc agttttgccc acctcttaaa aggtttatgt
    26521 actactcctt agcccttaaa aggatcgtct tactctcgta cactcagctt atctaacgca
    26581 ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg tcgcttcatt ttgtcctact
    26641 tttgtcacat agtatccttc cacccaaaag tgtcgatacc caaatttata ttttagattt
    26701 gcacaatctc ttgatgatct cctgaatatt ggctcgatat tgattataaa ttatttttct
    26761 tctatacttt ggtgtgaata caatgtggta tttacacatc tactttgtac gggctaaact
    26821 atttcccttt attgctccac ccgcatagca agtggttttg tatttcaatc gctcggcgcg
    26881 ctcaattagg tctaaagaca ataaatctaa aaacccgctg cttcactgac agaaacaacg
    26941 ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg caatctattt tatttttttg
    27001 attttttata aatggaaaat gcttcaagaa gtccaagaaa taaagccgta taaaaacaag
    27061 aacctatgaa atctttattt attagatatt caacagaaat cccgataaca ctagccaaca
    27121 atatatttat cagaagtgta aaataaggat gctttttata gaatagaact agtgttgttt
    27181 tcatagactc tcctttcttc atatttccaa ttaatcacaa attcacggca ttactagaaa
    27241 aattaaaggg gcgcagaagt ggaaaaatac tcttcgactt tccctacaaa ccaggccatc
    27301 ttgtgtttaa taactatacc aaatctaagg tacctctaaa tctaaattcc atttgtggcg
    27361 gtgacctcca atttttaact cttctatctc ccatccagta tacataacct tgatacttcg
    27421 accacttata tatcgaacac acaaatacct atccattgca ggagtactac ctaacttagt
    27481 tgctacttgc atctaaataa cagctgaaaa gataggactt actcttagta aagtatctga
    27541 actattaatt tttaaactat catttttatc ctcaactaca tgcacatctg ttgcaacttc
    27601 actttctgtc tcatccagat aaatgattaa ggaatcggga attgcaatat cttcagctga
    27661 cgctggaata gttgagctaa attagtcatc atatctttat tataaaataa ccaacagcaa
    27721 aaaatacaat gaaaaacaaa cttggaacta atgtatcatt aataaatgtt gaaaaattca
    27781 gcaactcatt actgtatgat tttagcagcc caaccaacat ataccctata aaagctaata
    27841 agatggttag gaatccttta gaattttt
//
LOCUS       DACEMS010000027        26632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEMS010000027 DACEMS010000000
VERSION     DACEMS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.027"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..403
                     /locus_tag="IW735_001017"
     CDS             5..403
                     /locus_tag="IW735_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1044033.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IW735_001018"
     CDS             387..1082
                     /locus_tag="IW735_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1044034.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="IW735_001019"
     CDS             complement(1666..2553)
                     /locus_tag="IW735_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1044035.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="IW735_001020"
     CDS             2738..3577
                     /locus_tag="IW735_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1044036.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4747
                     /gene="sgrA"
                     /locus_tag="IW735_001021"
     CDS             3773..4747
                     /gene="sgrA"
                     /locus_tag="IW735_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1044037.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5045..5401
                     /locus_tag="IW735_001022"
     CDS             5045..5401
                     /locus_tag="IW735_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044038.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5404..6561
                     /locus_tag="IW735_001023"
     CDS             5404..6561
                     /locus_tag="IW735_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044039.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6587..7027)
                     /locus_tag="IW735_001024"
     CDS             complement(6587..7027)
                     /locus_tag="IW735_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1044040.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7187..7693
                     /locus_tag="IW735_001025"
     CDS             7187..7693
                     /locus_tag="IW735_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1044041.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7861..9774
                     /locus_tag="IW735_001026"
     CDS             7861..9774
                     /locus_tag="IW735_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1044042.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9779..10780
                     /locus_tag="IW735_001027"
     CDS             9779..10780
                     /locus_tag="IW735_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1044043.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10982..11188)
                     /locus_tag="IW735_001028"
     CDS             complement(10982..11188)
                     /locus_tag="IW735_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1044044.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11834..12682)
                     /locus_tag="IW735_001029"
     CDS             complement(11834..12682)
                     /locus_tag="IW735_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1044045.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12846..13748
                     /gene="gnd"
                     /locus_tag="IW735_001030"
     CDS             12846..13748
                     /gene="gnd"
                     /locus_tag="IW735_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1044046.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13750..14751
                     /locus_tag="IW735_001031"
     CDS             13750..14751
                     /locus_tag="IW735_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1044047.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14748..15032
                     /locus_tag="IW735_001032"
     CDS             14748..15032
                     /locus_tag="IW735_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1044048.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15065..15862
                     /locus_tag="IW735_001033"
     CDS             15065..15862
                     /locus_tag="IW735_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1044049.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15876..16709
                     /locus_tag="IW735_001034"
     CDS             15876..16709
                     /locus_tag="IW735_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1044050.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16727..17584
                     /locus_tag="IW735_001035"
     CDS             16727..17584
                     /locus_tag="IW735_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1044051.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17606..18115
                     /locus_tag="IW735_001036"
     CDS             17606..18115
                     /locus_tag="IW735_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1044052.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18152..18553
                     /locus_tag="IW735_001037"
     CDS             18152..18553
                     /locus_tag="IW735_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044053.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18665..19018
                     /locus_tag="IW735_001038"
     CDS             18665..19018
                     /locus_tag="IW735_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044054.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19052..19495
                     /locus_tag="IW735_001039"
     CDS             19052..19495
                     /locus_tag="IW735_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1044055.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19512..20597
                     /gene="uxuA"
                     /locus_tag="IW735_001040"
     CDS             19512..20597
                     /gene="uxuA"
                     /locus_tag="IW735_001040"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1044056.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20590..21303
                     /locus_tag="IW735_001041"
     CDS             20590..21303
                     /locus_tag="IW735_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1044057.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            21632..22933
                     /locus_tag="IW735_001042"
     CDS             21632..22933
                     /locus_tag="IW735_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1044058.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            23556..23756
                     /locus_tag="IW735_001043"
     CDS             23556..23756
                     /locus_tag="IW735_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1044059.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            24324..24887
                     /locus_tag="IW735_001044"
     CDS             24324..24887
                     /locus_tag="IW735_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044060.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(24948..25292)
                     /locus_tag="IW735_001045"
     CDS             complement(24948..25292)
                     /locus_tag="IW735_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044061.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(25389..25634)
                     /locus_tag="IW735_001046"
     CDS             complement(25389..25634)
                     /locus_tag="IW735_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1044062.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(25704..25958)
                     /locus_tag="IW735_001047"
     CDS             complement(25704..25958)
                     /locus_tag="IW735_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044063.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(26122..26445)
                     /locus_tag="IW735_001048"
     CDS             complement(26122..26445)
                     /locus_tag="IW735_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044064.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT         9232 a         4165 c         5391 g         7844 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag
     4381 cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg agagtagcac
     4441 aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg atttggtacc
     4501 tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa gtgtgctaaa
     4561 acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag aaacagataa
     4621 tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa ttacatggat
     4681 gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct tccatcgagt
     4741 gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt tttgagtgaa
     4801 tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag atctattttt
     4861 gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa ataagagcaa
     4921 gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt aacgaatcta
     4981 tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa aaggagtatg
     5041 tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat ttttgaacta
     5101 ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag atctaaacta
     5161 caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg aaaggtatcc
     5221 agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt tttcttctca
     5281 gtttttactg agtaaagttt attctatact tttagaggga acgattggtt atattttact
     5341 ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa aaattatata
     5401 agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa aaactatcat
     5461 ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg tatccgatac
     5521 ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat attatcaagt
     5581 ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata tacgcgtagt
     5641 atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat gaaacaaggc
     5701 cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt gataacaaaa
     5761 gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg gtcgatgaat
     5821 cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat gaacacctat
     5881 ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg ctggattgaa
     5941 tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac ttcagaaaaa
     6001 aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt tgggacaaaa
     6061 aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca tatatttcca
     6121 gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat tccagagctt
     6181 aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt agtcagggat
     6241 atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca
     6301 caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt
     6361 gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca
     6421 tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg
     6481 gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa
     6541 aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa
     6601 aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt
     6661 gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa
     6721 gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct
     6781 caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa
     6841 tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa
     6901 attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga
     6961 cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa
     7021 tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg
     7081 aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta
     7141 gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc
     7201 cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca
     7261 cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg
     7321 gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg
     7381 ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc
     7441 gaattcattt tataagacta atttgagtgt tttattaact gaattggact gtcattcgat
     7501 cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg
     7561 attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat
     7621 gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt
     7681 tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat
     7741 tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt
     7801 tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga
     7861 gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta
     7921 agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct
     7981 cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca
     8041 caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa
     8101 acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac
     8161 gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc
     8221 ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca
     8281 gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact
     8341 gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact
     8401 tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt
     8461 acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt
     8521 catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc
     8581 gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac
     8641 gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt
     8701 gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca
     8761 gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta
     8821 ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct
     8881 gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt
     8941 tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac
     9001 atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt
     9061 gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca
     9121 ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa
     9181 aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat
     9241 ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa
     9301 gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc
     9361 aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa
     9421 aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat
     9481 gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag
     9541 caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca
     9601 agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa
     9661 aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga
     9721 tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat
     9781 gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac
     9841 tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt
     9901 tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga
     9961 acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga
    10021 tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg
    10081 cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga
    10141 cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac
    10201 accagttatc caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc
    10261 agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata
    10321 tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga
    10381 gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac
    10441 tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga
    10501 taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga
    10561 tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat
    10621 ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc
    10681 ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca
    10741 agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag
    10801 gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca
    10861 tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta
    10921 ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa
    10981 gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa
    11041 gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt
    11101 tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa
    11161 aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata attgtatgat
    11221 aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc
    11281 tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag
    11341 gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa
    11401 aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt
    11461 attttacata acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatgag
    11521 gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt taatttttta
    11581 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
    11641 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
    11701 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
    11761 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
    11821 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
    11881 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
    11941 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
    12001 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
    12061 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
    12121 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
    12181 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
    12241 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
    12301 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
    12361 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
    12421 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
    12481 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
    12541 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
    12601 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
    12661 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
    12721 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
    12781 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
    12841 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
    12901 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
    12961 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
    13021 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
    13081 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
    13141 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
    13201 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
    13261 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
    13321 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
    13381 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
    13441 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
    13501 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
    13561 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
    13621 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
    13681 cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg
    13741 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
    13801 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
    13861 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
    13921 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
    13981 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
    14041 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
    14101 aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga
    14161 tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt
    14221 ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat
    14281 gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa
    14341 gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca
    14401 gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat
    14461 ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa
    14521 agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat
    14581 catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg
    14641 gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac
    14701 cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa
    14761 tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta
    14821 tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg
    14881 gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc
    14941 aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta
    15001 caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga
    15061 tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc
    15121 tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga
    15181 agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt
    15241 gattgattcc cctgttccat atttcattgc cgggttagca gatacactag caaaatggta
    15301 tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc
    15361 acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca
    15421 agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc
    15481 tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc
    15541 tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa
    15601 agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga
    15661 gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt
    15721 tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt
    15781 gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga
    15841 aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa
    15901 gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca
    15961 cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg
    16021 aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt
    16081 ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg
    16141 cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc
    16201 tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag
    16261 acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa
    16321 cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc
    16381 cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag
    16441 tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa
    16501 aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag
    16561 atattacgat ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt
    16621 cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg
    16681 acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat
    16741 ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct
    16801 gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact
    16861 actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc
    16921 agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat
    16981 tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga
    17041 gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc
    17101 aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt
    17161 accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt
    17221 gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc
    17281 tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg
    17341 gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt
    17401 tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat
    17461 attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt
    17521 cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt
    17581 ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg
    17641 tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt
    17701 tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg
    17761 aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga
    17821 tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta
    17881 cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa
    17941 aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga
    18001 aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc
    18061 atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa
    18121 tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg
    18181 ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt
    18241 tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta
    18301 cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa
    18361 agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga
    18421 ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt
    18481 cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct
    18541 gctatccgta taaacaggta tcactgtact agcatttggg agctactttt tcttattggg
    18601 aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt
    18661 aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga
    18721 tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc
    18781 tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta
    18841 tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa
    18901 ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt
    18961 acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt
    19021 aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg
    19081 taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat
    19141 tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga
    19201 aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc
    19261 aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc
    19321 cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat
    19381 gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt
    19441 aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga
    19501 aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat
    19561 taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa
    19621 tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc
    19681 aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga
    19741 cagatgaacg agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat
    19801 gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt
    19861 tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc
    19921 agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc
    19981 cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa
    20041 tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt
    20101 gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg
    20161 gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt
    20221 ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt
    20281 tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg
    20341 agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg
    20401 atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg
    20461 atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg
    20521 caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg
    20581 gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa
    20641 actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg
    20701 cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg
    20761 ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat
    20821 aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt
    20881 ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta
    20941 aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca
    21001 ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa
    21061 atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca
    21121 gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat
    21181 cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac
    21241 tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc
    21301 taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca
    21361 gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta
    21421 gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg
    21481 agaatcgtcc atatgagggt ttccacggct ctataatttc taggactgat agaaaatcat
    21541 tctatcggtc ccttttgagc atgaaaaaac aaaacataca gaagctccgt tagaattgaa
    21601 tcaccacaac acaatcaaag gagcgttctg tatgcctgat cttcatcata tcaaactact
    21661 acttggaatc aaagataaaa acattttcat cacaaatgtg gaatcgaaga gcattaagaa
    21721 tatcaagagt cttgttgttt ctgcgacgtt aagtaaagaa attcgtcgct gtcctttgtg
    21781 taaacagatg aatcatgaag gcatgatcgt taagaacgga aagaaaaaat cgctcattca
    21841 acttaataaa tgtgcgaacc aactgactta tcttgcttta gcgaaacaac gctaccactg
    21901 tcgtggctgc cacacctatt ttacggcgaa tacctacatt gtcgatagaa actgctttat
    21961 cgctaaacaa gtgcgctata aaattttaga agagctaact gaaaaacaag caatgacgac
    22021 aattgctaag cattgcggcg tttcttggtc gaccgtttct cggacactcg cttctctgat
    22081 ccccatgaca aaagtaaaaa gaaattggtt accgcgttgt ttattaatgg atgaatttcg
    22141 ttcactgaaa aatcaagtcg gtccctactc cttttcttgt atggatggcg atactggaaa
    22201 attactggat attcttcctt cacggaagaa aaaggctctc gtttcttatt ttatgcaatt
    22261 tgaacgacgc gcacgattga aggtaaaggt catcgtgacg gacatgaatg tttcttatgc
    22321 ctctctgata aaggaatgtt ttccaaaggc aaaactcgtt gtggatcgtt ttcacattgt
    22381 caaacatctc attcgtaaat ttgaggatat tcgcgtacga atcatgaaaa gctttgatcg
    22441 aaacgatcca acacaagcca aatattatcg acaattaaag gcgttgtctc ggcttctgat
    22501 caaacgacag gacaagcttg tttatgacaa atggaccaaa tggcgcaatt ttggttgggc
    22561 gtatctcacc gaaagtgaag tggtggatcg tctgctttcg atctctgacg aattacgaat
    22621 cgcctatttt tactatcaag aaattcttca agcgtttcac gacaaagagg cggacacttt
    22681 ctttaaactt gtaagaacga tgcccaattc cgttccaaaa gaactgcatt ctataaaaaa
    22741 agcttttata aactatgaat ccggcattcg tttagccctc gaattgcctt attcaaatgc
    22801 caaaatagaa aatctgcata cccacatcaa agcattgaaa cgagtcgctt atggctttcg
    22861 aagctttcgg aaaatgaaga ccagaatttt cttactaaat aatctgatta cctatgaatc
    22921 aaaaaacatc taaagtagaa aaggaattct accttagatg ttcttatctc actctatcag
    22981 tcctatttga caaagagccg tttccactcc ttatgcagat ttttttaatt cttgcactgc
    23041 ttgttcgatc gtaacacctg tcgctacatg attggtattt tctttgtcaa aaacgacaaa
    23101 tagattcaaa tctgtatcca aggttactcg atattgtata tctttgttat agaatgtcat
    23161 agccattctc ctttctttaa atacttgctg aaaggcaacg aacgcgttga taacgaaatt
    23221 ccatttataa gtataccacc aattgaaagg ttttcatagt taagaaattt taaagtcaaa
    23281 agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa
    23341 ctttttcttc gattcgaaga caatagaaga caatgaattt tatatttcat tgcattatcc
    23401 ttgtgttatt agaaaaagca tgatatatta aatgagtaat ctattttgaa acgtaatttg
    23461 agcgatatat ctacatagat aaaactcctt ttgcctatca aatatttatt gcaacgaaaa
    23521 aacgtacatt gatacgtatc aggaggaatt tatttatgaa taacggtaca gtaaaatggt
    23581 tcaacgcaga caaaggtttt ggctttatca ctggtgaaga tggaaatgat gtatttgcac
    23641 atttttcagc tatccaaggt gacggcttca aaactttaga agaaggccaa gcagtgtctt
    23701 acgatgttga agatggtcaa cgtggccctc aagcaacaaa cattgttaaa gcttaataaa
    23761 aattgacttt taaccgtctt ttcgaaagaa aagacggttt ttttgtacga aaaaattcat
    23821 tggtcgttga atgggctgaa tagtagtgta taaaacaatt ttgtggagcg tgaagctttt
    23881 atggtagagg aaaatacgaa taaagtgatg ttgataaaaa gattcctatc ttgaatatga
    23941 atcgagatgt cttgaaaatg aatgagtttg accaatcatt atcctatcga aatttacagt
    24001 ttcgttcgat tggtctgtca attaatgaaa aagtaaaaaa gaatatgttc taggaagagg
    24061 gcggcacatc taaattttta agtaacggac agttcaaata tcaagcattg aaagaggcta
    24121 tcctactgtt tgaaggaata cgaatatata aaaacgacaa agaggaaaaa gtagttcatt
    24181 aaatcccttc aagtgatgaa agaatgagat ctaataaaac ctgatctttc atctgttttt
    24241 ttaataacgt aataattttt ttattttttg tgctttcgcc gttattgtag agtgatttag
    24301 cattcttatt tttaaaaata actgtgttat atattaaaaa aggcataata gttataagct
    24361 tttttaaaga atatttagaa attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta
    24421 ttattttagt ctattttcag ggggggaacc ttatgataaa aaaactattt ttactatcaa
    24481 tattaacgct agcaatggca ggttgttctg actctggctt agcaactgaa ctgatcacaa
    24541 aatcaactga agccacgaca gaggagttaa aatgtggaaa cgatcacagt gactacagag
    24601 gcttaaatcg acgatgcatg ggaatattag gcagaagcaa ttgtaagcat cgatgtagta
    24661 atcgaagaag gtgtagaaga agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc
    24721 tgtgctgtgc atcgtataca gacgtcgaag atgcggtact tttaaaccac gtcaggagat
    24781 gttcatttat gtctagggaa gttggcgatg aaccaaaact agagtacgct tttagttttg
    24841 cacgtgagga tgaagataac cctaaaactt acgacatttt agcttgatct aagatggtta
    24901 aagcttttta atgaagtaag aggagataga aggcttttct agttatatta ggtaagataa
    24961 attccttttt gctccaaatt tgctaaacat tctgctagat aaacttcatc gtgttccgga
    25021 taaatcggca cgttgcgctc tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca
    25081 cgatacatag gatttttcca ctctggtgct gggctgtaac cacgtttttc catttcatcc
    25141 atgacaagct gatgatattg gaataatcta taaggagaat agtgaaaaac ataatccaca
    25201 gtagcatgtt tctttcccca gccatttcct cttaatgcac agcattcccg atgttgtcct
    25261 aaaagctgtt gacgaggaag ctttgaaatc aatttttcat gccataatca catagaaatg
    25321 tctcctttat atatagattg ttaaccttag acgttaaatg aaaaaaccag caccaaggct
    25381 ggaaaaaatc acttcagttt gcttgctgtt tctgagcgac caattaggta atcaacggaa
    25441 atatcgtaaa agtcagcgat atagattaat tcaatgatgc ttggaatttc ttctccttct
    25501 tcgtacttac gatatttgaa taaaggaata cttaacgcgc tagaaacagc tacttgagat
    25561 aaagatcggc cagttcttaa gaaattcaga cgacaacgta aagtatccag taaaatagct
    25621 tcttcgatac tcatttaatc acctttcatt atagtagaat agttataatg actatagaaa
    25681 gctacacatg caatcaatag tactcaatgt tttttattta aacgtatcaa ataaagaatg
    25741 acgatcaata aagacaaaac cagtcctgat aattcttcta taaaaacact atcgggttca
    25801 aagtagttgt aattaaaaac tttccacata aagtagatag ccggaagagt caaaatagct
    25861 aaataaaacc gttctttgtt ccaattcata agataaacaa tacttgcgac cagtgcgacc
    25921 gcaggcaccc aaaacaaaac tctcaccaac gtaatcatgt aacggactcc tcaacgattc
    25981 atataataaa agataacatg ttattgcata catcacaaat gaaatgctag gaaaataaaa
    26041 gaagaataga tcaatgaaaa atgaaaaagc agaacagttt tctgctgata agcaggaact
    26101 gttccgctga atgtgtctta tctatcgaga aaaaatcaat ggattgatat ctaagtaatt
    26161 caatacatat aaaaagagga cgatcgcatt cactaaaaag gaaagaatga tgatcccata
    26221 gcgaatggaa acatatttaa caaatacttt tgtttgaata cgggcatatc tttttccttt
    26281 atagctgagc atcatgacaa gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat
    26341 aaacagaatt cctgaggttt gataatttaa ttggagaaaa taagctgctg ccgcaactaa
    26401 atcgaataaa atcgcaataa atggcaaaat aatcactcct aacattgaga ataaataaaa
    26461 gaataacatt ttttctaaaa taagcaaata aaaattaaag taacaaaata gttatgacac
    26521 aagtaaaaga aataagaata acaatattat tttacttata ttttgaaact tgtgagagtt
    26581 tgtcattcgt gctatagttg aaaagtaaca aaaataaatt ttatttatga tg
//
LOCUS       DACEMS010000028        26352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEMS010000028 DACEMS010000000
VERSION     DACEMS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.028"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(98..1228)
                     /locus_tag="IW735_001049"
     CDS             complement(98..1228)
                     /locus_tag="IW735_001049"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1044065.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IW735_001050"
     CDS             complement(1877..3079)
                     /locus_tag="IW735_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1044066.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IW735_001051"
     CDS             complement(3139..3342)
                     /locus_tag="IW735_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1044067.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IW735_001052"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IW735_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IW735_001053"
     CDS             4879..5691
                     /locus_tag="IW735_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1044068.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VQIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="IW735_001054"
     CDS             complement(6715..6867)
                     /locus_tag="IW735_001054"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044069.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IW735_001055"
     CDS             complement(7058..7936)
                     /locus_tag="IW735_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1044070.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="IW735_001056"
     CDS             complement(7975..9093)
                     /locus_tag="IW735_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1044071.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="IW735_001057"
     CDS             complement(9090..11189)
                     /locus_tag="IW735_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1044072.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="IW735_001058"
     CDS             complement(11240..12061)
                     /locus_tag="IW735_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1044073.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="IW735_001059"
     CDS             complement(12058..12867)
                     /locus_tag="IW735_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1044074.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="IW735_001060"
     CDS             complement(12888..13382)
                     /locus_tag="IW735_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1044075.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="IW735_001061"
     CDS             complement(13394..13882)
                     /locus_tag="IW735_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1044076.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="IW735_001062"
     CDS             complement(13959..16655)
                     /locus_tag="IW735_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1044077.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="IW735_001063"
     CDS             complement(17083..18156)
                     /locus_tag="IW735_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1044078.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="IW735_001064"
     CDS             complement(18153..18980)
                     /locus_tag="IW735_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044079.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="IW735_001065"
     CDS             complement(18981..19787)
                     /locus_tag="IW735_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044080.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="IW735_001066"
     CDS             complement(19799..20884)
                     /locus_tag="IW735_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044081.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="IW735_001067"
     CDS             complement(20901..21443)
                     /locus_tag="IW735_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1044082.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="IW735_001068"
     CDS             21701..22690
                     /locus_tag="IW735_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1044083.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="IW735_001069"
     CDS             22687..23424
                     /locus_tag="IW735_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1044084.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="IW735_001070"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="IW735_001070"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1044085.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="IW735_001071"
     CDS             23988..24620
                     /locus_tag="IW735_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1044086.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="IW735_001072"
     CDS             24705..25352
                     /locus_tag="IW735_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1044087.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="IW735_001073"
     CDS             25357..26340
                     /locus_tag="IW735_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1044088.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8060 a         5571 c         4465 g         8256 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatc
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcaaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacggtc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg at
//
LOCUS       DACEMS010000029        25943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEMS010000029 DACEMS010000000
VERSION     DACEMS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.029"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW735_001074"
     CDS             complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW735_001074"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1044089.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW735_001075"
     CDS             complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW735_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1044090.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(3549..4358)
                     /locus_tag="IW735_001076"
     CDS             complement(3549..4358)
                     /locus_tag="IW735_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1044091.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW735_001077"
     CDS             complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW735_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1044092.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(7968..8654)
                     /locus_tag="IW735_001078"
     CDS             complement(7968..8654)
                     /locus_tag="IW735_001078"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1044093.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(9002..10783)
                     /locus_tag="IW735_001079"
     CDS             complement(9002..10783)
                     /locus_tag="IW735_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1044094.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(10809..11723)
                     /locus_tag="IW735_001080"
     CDS             complement(10809..11723)
                     /locus_tag="IW735_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044095.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(11750..12712)
                     /locus_tag="IW735_001081"
     CDS             complement(11750..12712)
                     /locus_tag="IW735_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044096.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(12712..13656)
                     /locus_tag="IW735_001082"
     CDS             complement(12712..13656)
                     /locus_tag="IW735_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044097.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(13657..14661)
                     /locus_tag="IW735_001083"
     CDS             complement(13657..14661)
                     /locus_tag="IW735_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044098.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW735_001084"
     CDS             complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW735_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1044099.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW735_001085"
     CDS             complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW735_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1044100.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW735_001086"
     CDS             complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW735_001086"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1044101.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(18496..20178)
                     /locus_tag="IW735_001087"
     CDS             complement(18496..20178)
                     /locus_tag="IW735_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1044102.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(20230..20592)
                     /locus_tag="IW735_001088"
     CDS             complement(20230..20592)
                     /locus_tag="IW735_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1044103.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            20862..21050
                     /locus_tag="IW735_001089"
     CDS             20862..21050
                     /locus_tag="IW735_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1044104.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21288..23957
                     /locus_tag="IW735_001090"
     CDS             21288..23957
                     /locus_tag="IW735_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1044105.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(24048..24668)
                     /locus_tag="IW735_001091"
     CDS             complement(24048..24668)
                     /locus_tag="IW735_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1044106.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            24780..25181
                     /locus_tag="IW735_001092"
     CDS             24780..25181
                     /locus_tag="IW735_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044107.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            25316..25654
                     /locus_tag="IW735_001093"
     CDS             25316..25654
                     /locus_tag="IW735_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ1044108.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         7026 a         5499 c         4644 g         8774 t
ORIGIN      
        1 gaaaaaacac ataaattttt acatgcaaaa aagcttttga tcagattgac atcaaatagg
       61 acaatctgtc aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatt
      121 catcgtcaat cttctgcctt ttcctgcaaa gggataaaca tgcatatgca tggctggatt
      181 gaaatttgct tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc
      241 tggtataatc aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc
      301 gatttttttg taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact
      361 gatatttgag ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt
      421 caacccttgt tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg
      481 tgcacgatga ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac
      541 tgttcggata ccgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc
      601 tccgtcgatc acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga
      661 actgtcttca cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc
      721 cttgaaaatc gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg
      781 ctcggcaaat ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc
      841 tggtacccgg aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat
      901 aagtgggacg atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg
      961 gtcttggaat tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc
     1021 tttttggatg gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc
     1081 caactgatag ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac
     1141 ttcttgctga ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg
     1201 atgatccatt tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc
     1261 aaccagcatt tgcttcatcc cttcaagaat ttctctacct tcttgatgaa attctgtttg
     1321 atctgatggt tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg
     1381 gtcacagtct tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg
     1441 aacttgctct ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc
     1501 aatataacga acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag
     1561 cggtgtataa aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc
     1621 tgctaaatac tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc
     1681 cagcgcactg ttacgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta
     1741 gttcttctca ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat
     1801 aaagtttctc gccgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc
     1861 ttcctgatgg ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatggat
     1921 accgcttacc atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac
     1981 atcttcgaaa ttttttaatt ttgcaataat gatcgcttcg tctttttccg gtaaggcagg
     2041 tggcatactt aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata
     2101 aacagcagcg agatactgaa ataattcggg aatagaatcg gtaacaggag tgatcatctc
     2161 tgtttgtgtt tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat
     2221 tgctggatga tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc
     2281 tataccaaat cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt
     2341 aaaattcatc attaagtgac ctccaattca tatgaatcta attataatca aaaagtctaa
     2401 gaaaaagaac gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa
     2461 aaacacagct atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc
     2521 ctgcttttta gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa
     2581 atcatttgga tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac
     2641 tggtaaatgc aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa
     2701 ttttgtcaaa acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat
     2761 cgcgttttga ccagttgttg catctagtac aagaagtgtt tcttggggtg cttctggtaa
     2821 ttcccgctgg attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg
     2881 cagtcggcct gcagtatcaa ctagtaaaac atctgcctgc tctatctttg ctctttccaa
     2941 agcatcgaat acgacagctg ctggatcgcc gcctgcattg ccacgaacaa cttcaacacc
     3001 agcgcgttcg ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc
     3061 tgccatcaat acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt
     3121 tgttttccct acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat
     3181 atttagttca tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat
     3241 gatcgcattt tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa
     3301 tgcttcggtg attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc
     3361 taattcttca aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg
     3421 ttcaccaaat gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac
     3481 ttcttctgtt ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg
     3541 actcccccct actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg
     3601 ctggctgtca cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg
     3661 cctagtccag cgtattggat catcggtaaa tcattttctt catcaccgat tgccatgatt
     3721 tcttcttgtt tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca
     3781 ccttttggca taaattccaa aagcatactt ctcgttttga tgatctcata catttcatgg
     3841 aattctgctg gtatttcttt gattttagga tctaattctt cttggtcata agcgattact
     3901 gccttattaa gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc
     3961 agcttattca acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc
     4021 acattgtcag acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt
     4081 tggatatctg atacttccat cactttcttt tccatgattt caccagtatc atttttttgt
     4141 accaaaccgc cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc
     4201 agataatgca ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc
     4261 tgttttgctt gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat
     4321 aatgtaccgt ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt
     4381 cgttgatttt cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac
     4441 cggattcctg catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt
     4501 gtgtgactac gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt
     4561 tcacgttggc ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac
     4621 agacacggat aatagaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa
     4681 gcaaacttaa actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt
     4741 tcagcaaatc agttggttct gtcagtacta attcagcttt accgccgcca aacatatttg
     4801 gaaagacaat tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca
     4861 cttcatcatc catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat
     4921 ctcgctgtgt tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat
     4981 ttaagtttac tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac
     5041 tgtcttcgat atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat
     5101 attctgactg cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga
     5161 cttctaattt ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga
     5221 ctgcttcaag cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca
     5281 actcggcttt caatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt
     5341 cttctgtgac ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg
     5401 cttcttgacg ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca
     5461 gatgattaaa ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt
     5521 gtacttgtgc tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt
     5581 cgtcgatttt ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt
     5641 gcttttcgta atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct
     5701 tttctttttc aaaacgttca agttcattta tgatgttttg cagctgattt gtcgcttctt
     5761 gttcttcaaa acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac
     5821 gagctgtttc ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg
     5881 cttcttcaaa ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt
     5941 ggtttcctcg tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt
     6001 ctaaggaaac cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta
     6061 agtctttggc taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg
     6121 gataactgac ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca
     6181 atatatgagc cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc
     6241 ctcctctttg ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga
     6301 tgacatgctg ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa
     6361 catcgatcag ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga
     6421 ccaatcgaac cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct
     6481 tacgagcacg tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg
     6541 ctaatgcttc ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt
     6601 cagttaaaga tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca
     6661 atcggtctac tgatgcctct acttccgatt gcttggctaa cgtctgctta ctttttgctg
     6721 tttcttgtac atattgacgt tctagatact tcagttcgtt tcctacagct gcttgctctt
     6781 gcatcaaatc aacatattga tctcttagtt ctgccagtaa ttctttcgtt gatttttggt
     6841 atttttctaa ttcttgctgt gtttttatga gacttgcttc agctttttgg atttctgttt
     6901 ctttttctgc tacttctttc aacaagcttt cttgaagctt ttcaaaatgt ttaacctttt
     6961 tttgtgcttc tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc
     7021 gctcctgcaa aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata
     7081 atacttgctg atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg
     7141 ccaaaatgga ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt
     7201 tcgcaagctg tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcggcaacca
     7261 tcaaagagac atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct
     7321 cactctgggc ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc
     7381 ggctgaggtt gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt
     7441 atttcaagac accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga
     7501 aaatcgcttc tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagagt
     7561 ctaagaacaa ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc
     7621 tttccccagt ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat
     7681 aatgatcact attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat
     7741 cggaaccggc aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt
     7801 ctcctaatac ccagcggata gcttctgtaa tattgctttt tccactgcca tttggtccaa
     7861 ccactgctgt gacgctattt tcaaagtcaa tgactgtctt gtctgcaaat gatttaaaac
     7921 cggctatttc aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc
     7981 aatgcatttt ccgccgcagc ttgttctgct aattttttcg attttccttg tccagtgcca
     8041 atcaattgat catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct
     8101 tcattgatca atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt
     8161 tttgttttat gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact
     8221 ttttcgatga atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa
     8281 gcttcgaata ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc
     8341 ttgcccaaca aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct
     8401 tcgcgaacga tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa
     8461 cggaataaat actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc
     8521 tcattatccg ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct
     8581 tgttcaagca aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc
     8641 agctggttgt ccattttcca cattcctttt atggatattt tttcgatact tccttattat
     8701 actggttttt tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc
     8761 ctagtgattg acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt
     8821 tgtgaaaatg gctttccata tttttgcata aatttgactt aataccgaga aactggactt
     8881 tgaacaccgt ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat
     8941 aaacagaaaa atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg
     9001 attatgaacg tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag
     9061 accaatccca gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg
     9121 gaatcgcaaa tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt
     9181 actcttcatc aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg
     9241 attcataacg agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat
     9301 cagttcctgt attccaagca gcttggtaaa catcaatttc aggatcatca ttttctaatt
     9361 tatcatagaa agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccga
     9421 tttctttcca ctgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca
     9481 tcgaagcaaa gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat
     9541 ccacatcttt gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga
     9601 atcctttcat ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat
     9661 tgcttctcaa acctgcatag aaacgctcac ctactgcatt attatctaat gcatagccca
     9721 ttgcttgacg taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg
     9781 tatctgcatt ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc
     9841 ctaagtttgt atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga
     9901 aaatcatatc atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt
     9961 tcatcgtgat ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt
    10021 ctactgattc tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt
    10081 tacgtacttg atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg
    10141 gtgctgcata tgaccaaata ccaccacccg attgaagcat gcttgggttt acttctttat
    10201 attcgatttc aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc
    10261 catcatgata ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag
    10321 tgtaatcttt attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt
    10381 caccatctga ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat
    10441 ctagatcaag cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata
    10501 gcggttcatg agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact
    10561 ggaacaatcc tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga
    10621 ttgcatcatc attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt
    10681 tgcctccaga gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga
    10741 cagcagacat cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg
    10801 cttctatact agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca
    10861 taattgatac tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc
    10921 cgattcacta agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca
    10981 tttgccggca agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca
    11041 aagttcatcg tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac
    11101 atgattttaa aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct
    11161 tcagataatg tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg
    11221 atgaatataa aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc
    11281 agcatgatcg gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc
    11341 cctccgtagt agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg
    11401 atcgcaaatc cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca
    11461 cgtccgcctt catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt
    11521 gcagcgtatt tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca
    11581 ccgataaaga tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta
    11641 tcttttttaa attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact
    11701 gctgtattgt ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc
    11761 gaatacgcgg gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg
    11821 cgattcctaa aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat
    11881 caataaatag ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag
    11941 caatcaacga agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga
    12001 aaatatgtcg actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa
    12061 aatcgagcga tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg
    12121 ccaaaaatgc ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag
    12181 tgataaatcc accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac
    12241 gataaccaaa aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa
    12301 agaagttgta gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca
    12361 tccccagagg cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag
    12421 tgttgatgat ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac
    12481 caaaatcgcc ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat
    12541 tcaagccagc tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca
    12601 aaccggtaaa tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa
    12661 taataacttg cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc
    12721 acctccatct ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg
    12781 accagtcgca tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg
    12841 attttctttg tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg
    12901 tttcgtatag atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa
    12961 tcgacctttg tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg
    13021 tgaaatgaat aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac
    13081 ttgcgcttga acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg
    13141 attggtagca acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg
    13201 aggatattta tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt
    13261 tttcttttct tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc
    13321 caacactcgc tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc
    13381 cttgttataa cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat
    13441 gatttgtccg gctgtcacct tttctaatcc cacaacggct tttcccgtag tagattttcc
    13501 agagccagat tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc
    13561 atctacagca tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg
    13621 aactttcagg tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct
    13681 aaagtggaaa tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag
    13741 gataggctct tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca
    13801 acctttacga ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc
    13861 atcatgattt ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg
    13921 atgatcaaac agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc
    13981 cactcgatca gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt
    14041 ttttgtttct tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc
    14101 tagggctgtc gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc
    14161 gatgatgaca cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct
    14221 cgcaggatta ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc
    14281 tgcattcatc tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag
    14341 aggatttaat gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt
    14401 attatagagc gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt
    14461 aatttttgtg ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc
    14521 gcacccagat tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc
    14581 atctactgca tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc
    14641 taataattgg ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct
    14701 taataaaaaa ggacattaca cagaataaag cgttttataa caaaggttat tcttatcttt
    14761 gtgtaatgaa ttattaataa ttatatagaa aatcgcttta ctttgcaaca taatttctgt
    14821 ttatcgccag aattatcaga aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg
    14881 ataaaaagct gtatgcagct ttttatcctt ctttgctttt atttgaaatt atttaagatg
    14941 gctgctgata taatctactg cgccaccgac tgtttcaatt tgttcagcgt cttcatctga
    15001 aatttccgta ccgaattcat cttcaagctc taaaacgaat tccataatac taattgaatc
    15061 agcttttaaa tcatctttga tgttcaattc atccgtcact ttatcggtat ctatctcaaa
    15121 atgatttgaa ataattttcg ctactttgtt aaatacttct tcgcgagtca actttttcac
    15181 ctccaagaat catacacttg tcaattacct acaccatggc taattttact ggcatttttc
    15241 aggaatgtcc accttttttt caatggaacg gattgcttca gatatccaaa tactattctt
    15301 cgcttttttc aaattgttcg actaattgtc caactacatc tgtctcaagc attgtgtgga
    15361 tttggcggat cgtatagcga acagcatcgg ggcctgttgc tccatgagtt ttaatgactg
    15421 gtgctttcaa gccaaacaaa acggcaccgc catgtttgga ataatccatt tctgctttca
    15481 aagaacgtaa gccgtctttt aaaagcatag cgcccatttt tcctttgatc ccttcattta
    15541 gaatagcaga ttttaataga ttcatcatat tcattgctgt tccttcaata gatttcaaaa
    15601 cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc cacaccattg agtagctcac
    15661 gagcttctac gtttccaata aaattcaagc ttgtttcatt ctgaaggagt tcataggctt
    15721 ttttcgtcac ttcactacct tttgttgctt ctgttccgtt gtttaagagt gcgacacgag
    15781 gacgtccgat acctcggact ttacgtgcat aaaaagaacc taaaataccg tattgcaaca
    15841 aatgttctgg tttgttttct gcgttagcgc caagatccag catatcgaaa ccggcacctt
    15901 ctttccctac tactggcaag gtagacatca agccagggcg ttcgatattt ttgatacgtc
    15961 ctacgatgaa caaccctgct gctagtagcg cgcctgtatt ccctgctgaa aatacagcat
    16021 ctgcttcacc gtttttcact gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc
    16081 gaattgcttt tactggctca tcatcactat tgattttttc gtctgtatgg ataatcgtga
    16141 tgtttttttc atttgttaag tatttcttga tttctgcttc ttttccgtaa agtaagaatt
    16201 ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt
    16261 gatctccgcc cattgcatct acggcaattt tcattgtttc cactcctttt tccgtcatca
    16321 gtataaagta tagcacagtc tgctaatcga aataacgatt atcctgattt tctatttgga
    16381 ggctttgcgc taaaggctga tactcaggaa gcatctgcca atcctttatc tgccaaaggg
    16441 ctgcagcctc ttctctggct acctccaaaa catttccatc cgtcaccaag tcagcaatcg
    16501 caaattgagg cagaccagat tgacggaagc caaatacttc accaggacca cgtaattcca
    16561 gatctttttc gcttagtaca aaaccattgg tggtctctgt catgattttc atccgttcca
    16621 cacctagttc atttttagga ttagcaacta agatacagta ggaagcatca cttccgcgtc
    16681 caacacgtcc acgaagttga tgcagctgtg ccaatccaaa tcgatccgca tccatgatca
    16741 gcatgacggt cgcgttcggc acgtttaccc ctacttcaat tacagtagtc gaaactaata
    16801 tctgcatctg gttgtctttg aaggcttcca tgattacttc tttttcctga ttcttcattt
    16861 taccatgtaa caagccaact tcatattttg gtgcaaatag ttcacgaagt ttttcataga
    16921 tttccactgc attttttaca tctaatgctt ctgattcttc aatcagcgga cagatgacat
    16981 acatttgatg tcctcttgcc agttcttttt tcgtccaatc cagtacactg tccaattgag
    17041 gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc tggaagttca tcaatgatag
    17101 atacatccat ctcaccatat gctgtaatag ctaatgttct aggaatcgga gtcgctgtca
    17161 taaataagac atctggtcgc cagccttttt cacgaagtac tttccgctgg ttcacgccaa
    17221 aacggtgctg ctcatccgta ataactagac ctaaattctg aaattcgact ccatcttgga
    17281 tcaatgcatg tgtgccgatc acgatgttaa tttccccttg ctctagtttt tcaaggagct
    17341 cacgacgttc ttttgctttc gttgaccccg taagcaatgc gatggttact tcattgggat
    17401 caaacagctg ttgcaagctt tccatatgtt gttgtgccaa gatttctgta ggcaccatca
    17461 acgcaccttg gaaacctgct gtaacggtag cgtataacgc aatcgcggca accactgttt
    17521 ttccgctacc tacatctccc tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc
    17581 gacagatttc attcgttaca cgtttttgtg catttgtcag ttcaaaaggt agcttttgag
    17641 taaattcttt caatttttgg acatcataaa ggatctccaa accattcgtt tctgcttttt
    17701 ctgctttttt caatccttga agacgcattt gaaataaaaa gaactcttca aaaacgacac
    17761 gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa atgcatactt cttacagcag
    17821 aagctcggtc aagcagtcga tatttctcca caaggttgtt tggcagattt tcttcaatca
    17881 atgtgccgta ttcttgaaac ccttttttga ttagatcaac taatgtcgtt tgacgaattg
    17941 atttgttgac gtgatatatc ggcgaaaagt catccattgt ttgagaacct aaaatcttca
    18001 tcccgttcaa tgctttacgc tttgcatccc atttaccgta tacggcgatt tcctccgata
    18061 aaatgacttt ttcttttaga taaggttgat taaaaaaaga aacgttgaac acatcatgtt
    18121 cttgcatcat acgaaattgc aatcggcttt ttttataacc aaatcgattc atcacaggag
    18181 gcgagactac gattcctttc aaagtaacct tttcttgatc ttggatctcg tttagatttc
    18241 tttcctgaat atcttcgtag cggaaaggat aataagtcag taagtcttca atcgtttgga
    18301 tgcccaagct ggcaagacta gctgctcttt tttctccgac tcctgccagc atagtgattg
    18361 gatcatctaa agaacgcata aatttttcct ccctccctta acaaaaaaag gacttggtca
    18421 gcgatcatac ctgatcacta accgaaggcc ttttatgaat gaatactgtg ttagaaagat
    18481 attctgacac agaaattatt cagcagaaaa tagataagga tagacaggct gatctccttg
    18541 gtggatctcg atttccaaat caggatgttt ttctgttagt ttctcagcaa attcatttgc
    18601 ttgttcaact gttccttcat cacctgccaa gatcgttacg atctctgtgt cctcattgat
    18661 catttgttct agtgtactta acgctgttgc tgtgatatct gggttagaga tcaggatttt
    18721 tccatcgatc atgcccaaaa agtctccttc tttgatctct acaccgtcaa ttgaggtatc
    18781 tctgatcgcg tgagtgactt gaccgctcac aacgctagac aacatttctg tcatcgtttc
    18841 tttgttttct tctaaagaag cttgatcatt gaatgaaagc atagctgtca atccttggga
    18901 aatcgttctt gaaggaacaa ctgctactgg gatatctgct acttctgctg cttgatcagc
    18961 tgccatgaag atgttcttat tgtttggcaa gacaatcact ttatctgcgt ttacttcttg
    19021 aatcgcttta acaatgtctt ccgtacttgg attcattgtt tgcccaccac tgataacata
    19081 attagcgcct aaactttcaa acagctcttg gacgccttta ccagcagcga tcgctacgat
    19141 accatacggg atacgtggtg tttctttttt cgcaaactcg ttcacttctt catcgtgttc
    19201 caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg attttgacta atgaaccgaa
    19261 tttttgtcca taattcaaga cttctcctgg gttttctgta tgcacgtgga ctttgatgat
    19321 ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca tctaaatagt tacggaattc
    19381 ttcgtaatcg aatgtactat cgactgtagg tccttctccg atttttacca tgatttctgt
    19441 acagtaacca aaatggatat cttctgtcgc catttgacct tggacactgc gatgatgttc
    19501 tgcattgacc atatcatcca tttctgctgg agaaggttca tatgtttcat ccgcttggaa
    19561 ttctccattc aatgcactta aaaagccctc ataaacaaac agcagtcctt gccctccact
    19621 gtctacgaca ccgacttctt ttaggactgg aagcaagtct ggagttttag ctaaagcacg
    19681 ctttgcccct tttacaacag ctgtcattac ttcaacgaca tcatcagtac tttttgcttt
    19741 cttttctcca gctttcgctg cttctctagc tactgtcaaa atcgttcctt ctactggttt
    19801 cattactgct ttatacgcag tttcaactcc atgagtgaag gctgcagcaa gatcggaagc
    19861 atttaatgag acaacatctg gaatttgctt agaaaatcca cgaaacaatt gagacaaaat
    19921 aacgccggaa tttccgcgcg cacccatcag caatcctttt gaaagcattg ctgctaattc
    19981 tcctactttt tctgaggttg aatctactac tgccttagct ccactagtca tagatagatt
    20041 catatttgtt cccgtatcgc catctggcac agggaataca tttaatgaat tgacgtattc
    20101 tgcatttact tgcagacgat tcgcacctgc ctggaccatt tcctggaact ggcttgcact
    20161 tatttctgtt acactcacct agataatcct ccttcagatc gactgctgag ttaccagcca
    20221 gtctcctgcc tagtctggca gtacacgaac accttgtaca aagacattaa ctgaattggc
    20281 agttacgcct aataatgcct ctagattgta tttcactttt tcttggacgt tgcgggaaac
    20341 ttcagagatt ttcgtaccat agctgataat cgtataaaca tctacagcaa taccattatc
    20401 ttcttggcgg acaactactc ctcttgagta attttcttta cgcaaaatct cagtaatatt
    20461 atctttaatt tgatttttgc tggccattcc tacaacaccg aacacatctg tcgcggcgcc
    20521 accaacaact gttgcaatta catcattggt aatttcaatg gttccagctg acgtttgaat
    20581 ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac tcccattttt cttctatcat
    20641 attttatcat agcgcaactt gaaataaaag gcagttaccc aaagtactca aaaaccttgc
    20701 aaaatttcag gtataacctt gtttaattta caattattct tctcaattgt caattgtatc
    20761 tatgaaaatg tacttgcaaa tttccattct atatgatatg ttagtctggt atgagctgaa
    20821 ttattctatt gctccaaatc aaggcaaagg aggaaattac catggcaaaa gtttgttact
    20881 ttacaggtcg caagacaaaa agtggaaaca accgttcaca cgctatgaac tcaacaaaac
    20941 gtactgttaa acctaactta caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag
    21001 tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata
    21061 cttggaccgt tcagatagtg aacggttttt tatttaataa aaatttttaa gggtgggact
    21121 tttgattcct accctttttt tgcgtttatt tttgttatac gaacaaatta tgaagttatt
    21181 tattctaaat tttatttttt tgtgaaaatt agactctttt taagcagaga aaaacaagga
    21241 tatgataaac tgaatacaac ttcatagaaa tttggtggaa aataaatatg aaaaaaaatt
    21301 taaagtcatt ctttagagaa aacggacgct acgcagcagc tagtttcctg attccgtttt
    21361 tgattttagc acttgtttat ctaagtatcg ggatctatcc tggaagcagc agaagtattt
    21421 tggcaagtga tgccttttca cagttttcta attttcatgc cagcttccga aatgttcttc
    21481 ttggaaagca aagtattttt tatacatgga atgcttctct aggattgaat tatttatctt
    21541 taatttctta ctatctaggc gggatcttca caccactggt gttctttttt ccaaaccaga
    21601 tgatgccaga tgcactgtat gttctgactt tgcttaaggt cggctgtgcc ggattggctt
    21661 tttggtttct tgcacgtacg ttcaagattc ctcgctgggg gcatgttgca cttagcgtca
    21721 gttacgcatt gatctcattt atcactgcac attcggagat catcatgtgg ctggatgcat
    21781 ttgtttatct tccattagtg attttgggga ttcatcgtgt tatggatctt aaacggccga
    21841 aacttttatt tatcagttat ttactcttgt ttttatccag tttttatatg ggttttatga
    21901 ttggcgtatt ttctttctta tattttatcg cccgtctatt gacaaactgg tcagaatata
    21961 aaaaaacgat tgttccttat ggtatcactt ctttactggc aggcggcgct tcaatgatca
    22021 ttgtattgcc agcagtgctg gatcttcggt caaatggcga ggcactgagt cagatcacta
    22081 cgttgaaaac agaagcgact tcttatctgg atctaatcat gaaaaatatg attggtgttt
    22141 atgacacgac aaaatacgga tcgattccgt tcgtttacgc aggtctgcta ccattagtcc
    22201 tctgtctgtt ttattttgcc agtcggaaag taccgttgaa aaataaattt ttgtacggca
    22261 gtcttttcgc catacttatc gcaagtttct atcttgttcc attgaatttg ttctggcatg
    22321 gtatgcatgc accgaatatg tttttattcc gttatgccta tctgttctct ttcttagtgc
    22381 tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga cgatcgtggg ctgttgttag
    22441 gaatcatttt ggtcgtagct gctctctttg cggtggcatg gggcatcaaa cctgaaggaa
    22501 gctacaccta tgtgacgatg acttcctttg tattgacagt ggttttctta gggatttatg
    22561 cttttgctgt gggattttat cagcataaaa aactttcatt caatcatctg agtatattgt
    22621 tattattgat catgtctgct gaagcactgg tcaatacgaa cagtatgatc catggcattc
    22681 ttgatgattg gaattatgct tcccgcagtt tatatactga tccttatcct gacttgaaag
    22741 ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta ccgcttggaa aatctggatc
    22801 cagtatctgc aaacgattcg atcaactatg gttacagtgg tatcagtttg ttttcttcta
    22861 taagaaatcg taattcttcc tcttacctgg ataaattagg tttccgttca agagggacaa
    22921 atttgaatat ccgttataac aataatactt tgctaatgga tgggtttaca ggaatcaaat
    22981 ataacatttc taaaaacgaa agcacgttca gtaaatatgg cttccgccaa gctggtcaaa
    23041 gtggcgacta tacactgtat cagaatgtca atgcattgcc actagcattt actgcaccgt
    23101 tgtccgttaa tgatgttgag cagccagtga cagataattt gaccagtcag acaaacttga
    23161 tcaatacatt gtctggttta aatgaacagt attttacatt ctatacacct actttgaaga
    23221 atcagttgaa tacgacgatc acaaagacaa cgactggcgt cacttactca gaaacagcag
    23281 ataaccagcc aaaagagctc acttgggaag tccatgtgcc tgcaaataca caagcttact
    23341 taagtttgtt cccaaccaat tttgcacaat tagaaagttc tagtgcgacg atcactgtca
    23401 atggtacatc gcgaaaaacg caaatcaaca tttctggtca atactatgat cttggctatt
    23461 atcctcaaga gacaacagtg acatttactg caagttttta tggaacgaaa gaagtaagtt
    23521 tcatggagcc taaagtcgtt ggtctagatg ttgtagccta tcaagccgca atgaatcaga
    23581 tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa agcttctggg actgtaaata
    23641 ccgctgaaga ttccatgttg gtgacaacta ttccttatga caaaggatgg aaagctaaaa
    23701 ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc attcttgatg ataaaagttc
    23761 cagcaggtaa acatactgtc actttcagtt acttgcctga aggatttacg ataggtgcaa
    23821 tcctttttgt cctatgtatt gcattgttta ttctttatgt ctggtacctt gaccgcaatc
    23881 gcccctttta tggcacgatt gatccagcaa tagaagaaaa accgcgaaat acacacagaa
    23941 gaagacgaag aaaataaaga aacgatggaa tctgtgtcac tcttctattt aaatggcaaa
    24001 aatttcaatc tttaaatgac aaaaaccacc tgatttttgg tggtttttta tatacctatg
    24061 actaaactat ctcttgctgc ttcgacaact tctacagtga tcttatccat ttgattgatt
    24121 cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa gcctaaaatt tccttttgtg
    24181 atcttaatga tagttgtgat agcctcataa tcagaaaaat cttctagctt gatatcaatt
    24241 cctaaatctg cccatttgta ttccaaaata tgatgtgttt cgtccttact aaggttatca
    24301 aattcatgtg caaatcctat gcgagagtaa agctgcggat aacgagacaa tcttttttca
    24361 ataccaggca taccgataag aaccattgcc agatcatttc tatcgtaaat atctcttagc
    24421 tgttctaaat gctgaagctt taaacgatca atttcatcga caataattaa gtcaattgct
    24481 tcataggttt taggcacagg aggatcttct tgattgtatt ttagtaccat atgcattgct
    24541 ttagtcagac ctattctact agttattgtg ttaatatccg aactgacacg agacatcttt
    24601 tcagctggta cagtataaaa aactttattt acacttaata tttcatctgt tgcatttatc
    24661 aagaacaaga aaaagtctga atattcaaaa aataacggtg tacattttta aaaggagatg
    24721 tataatcgag atttagaaag cgttttccta aaaatttaat gaattaggaa gtgattacta
    24781 tgaaaaaatt atttagcgca ctttttattc tgttatttgg ctttattggt attgggactt
    24841 ctggaaatat agttaaagct agcacgatgg ttgaacaaac aagtgaaatg gcaaaaactt
    24901 ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc caaaggattt agtgataaag
    24961 atacttttta cactcaaaag atagagcctc aagatccgaa aacacgtttt tctgcaaatg
    25021 tattgcggtt aaccgcatct actaaaaaaa ttactaatac gcaggggcgg acagaatata
    25081 ttataacaag tcccaattcg gcgatgtatc gtatttctac acgcttgagt ttaggtagtg
    25141 caaaatcata tactcaaaca gtaaatctag ctaattctta aggttatggt ggaggaatgt
    25201 attttacatt tactgggaaa agacaatatt acagtgtaaa attagctatg caagtttata
    25261 atacatttgg tgtagggtca gtctcttgca ctgcgggagg attgacaata ggaaaatgat
    25321 aatttcttac tatgtcttaa atagaagtga tgatgaagga ctcttcgttt ttcaatcatt
    25381 acatgaggat acgatgctaa aagagtttta taatcggatt aagaataaac agatagaaac
    25441 aaatttatat tttttaagtg actctatctt gccttttatt agcaacccta aaattcttaa
    25501 cttgttgcag acaaaagatt tgaaaagttt accgattatt gtcattgatg aggaaattgt
    25561 aaaatatggc gcttatctaa gcgtagaaga attagaaaaa ttaactaaca ttggaatgag
    25621 tattcaggag gataatgggg tacatggaac atagaatgaa tatgcacagt tgagaacgga
    25681 atttgacatt tatttagacc aacagggtat accctgttgg tctttttgtg cgttcatgat
    25741 atacttggtg tcatttttat atttaaatgg cacaaatatt tctaattatt tgtcatttaa
    25801 cgctgaaaat ttttgctatt tatctaaaaa agtgacagga tactcatttg gccttcatag
    25861 aattttctaa atatgaatca tcaatgaatt taattaaaaa aataagtagg gcaaaatata
    25921 aatgtttttg ccttacttgt ttc
//
LOCUS       DACEMS010000030        25933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEMS010000030 DACEMS010000000
VERSION     DACEMS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.030"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW735_001094"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW735_001094"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1044109.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW735_001095"
     CDS             complement(937..1518)
                     /locus_tag="IW735_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1044110.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW735_001096"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW735_001096"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1044111.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW735_001097"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW735_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1044112.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW735_001098"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW735_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1044113.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW735_001099"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW735_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1044114.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW735_001100"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW735_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1044115.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW735_001101"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW735_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1044116.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHEYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW735_001102"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW735_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1044117.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW735_001103"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW735_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1044118.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW735_001104"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW735_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1044119.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW735_001105"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW735_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1044120.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW735_001106"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW735_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1044121.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW735_001107"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW735_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1044122.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW735_001108"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW735_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1044123.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW735_001109"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW735_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1044124.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW735_001110"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW735_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1044125.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW735_001111"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW735_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1044126.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW735_001112"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW735_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1044127.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW735_001113"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW735_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1044128.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW735_001114"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW735_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1044129.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW735_001115"
     CDS             13972..14157
                     /locus_tag="IW735_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1044130.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW735_001116"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW735_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1044131.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW735_001117"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW735_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1044132.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW735_001118"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW735_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1044133.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW735_001119"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW735_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1044134.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW735_001120"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW735_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1044135.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW735_001121"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW735_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1044136.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW735_001122"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW735_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1044137.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW735_001123"
     CDS             18232..18879
                     /locus_tag="IW735_001123"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1044138.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW735_001124"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW735_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1044139.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW735_001125"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW735_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1044140.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW735_001126"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW735_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1044141.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW735_001127"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW735_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1044142.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW735_001128"
     CDS             20317..21255
                     /locus_tag="IW735_001128"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1044143.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW735_001129"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW735_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1044144.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW735_001130"
     CDS             21935..22774
                     /locus_tag="IW735_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1044145.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW735_001131"
     CDS             22750..23619
                     /locus_tag="IW735_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1044146.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW735_001132"
     CDS             23609..24406
                     /locus_tag="IW735_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1044147.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW735_001133"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW735_001133"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1044148.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8412 a         4777 c         5585 g         7159 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcatgaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt ttggttgggc
    25561 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgt
    25621 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
    25681 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    25741 atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt tgaggaatat
    25801 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    25861 aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc ggaacaggtt
    25921 tgtcattttt ata
//
LOCUS       DACEMS010000031        25675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEMS010000031 DACEMS010000000
VERSION     DACEMS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.031"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            171..515
                     /locus_tag="IW735_001134"
     CDS             171..515
                     /locus_tag="IW735_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1044149.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IW735_001135"
                     /pseudo
     CDS             516..935
                     /locus_tag="IW735_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="IW735_001136"
     CDS             complement(1027..1377)
                     /locus_tag="IW735_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044150.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IW735_001137"
     CDS             complement(1343..2674)
                     /locus_tag="IW735_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1044151.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IW735_001138"
     CDS             2930..3496
                     /locus_tag="IW735_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1044152.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IW735_001139"
     tRNA            3641..3728
                     /locus_tag="IW735_001139"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /locus_tag="IW735_001140"
     CDS             4062..5036
                     /locus_tag="IW735_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1044153.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="IW735_001141"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="IW735_001141"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1044154.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="IW735_001142"
     CDS             complement(7451..7897)
                     /locus_tag="IW735_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1044155.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="IW735_001143"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="IW735_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1044156.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="IW735_001144"
     CDS             8362..8880
                     /locus_tag="IW735_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1044157.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="IW735_001145"
     CDS             complement(8957..9913)
                     /locus_tag="IW735_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1044158.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVRKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="IW735_001146"
     CDS             10079..10885
                     /locus_tag="IW735_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044159.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="IW735_001147"
     CDS             10882..11871
                     /locus_tag="IW735_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1044160.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="IW735_001148"
     CDS             11868..12872
                     /locus_tag="IW735_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1044161.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="IW735_001149"
     CDS             13004..13546
                     /locus_tag="IW735_001149"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1044162.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="IW735_001150"
     CDS             13566..14264
                     /locus_tag="IW735_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1044163.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="IW735_001151"
     CDS             14285..14497
                     /locus_tag="IW735_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1044164.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="IW735_001152"
     CDS             14497..15459
                     /locus_tag="IW735_001152"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1044165.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="IW735_001153"
     CDS             15477..15872
                     /locus_tag="IW735_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1044166.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="IW735_001154"
     CDS             16035..16400
                     /locus_tag="IW735_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1044167.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18208
                     /locus_tag="IW735_001155"
     CDS             16397..18208
                     /locus_tag="IW735_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1044168.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18270..18437)
                     /locus_tag="IW735_001156"
     CDS             complement(18270..18437)
                     /locus_tag="IW735_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1044169.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18685..19674
                     /gene="galE"
                     /locus_tag="IW735_001157"
     CDS             18685..19674
                     /gene="galE"
                     /locus_tag="IW735_001157"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1044170.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19686..21170
                     /gene="galT"
                     /locus_tag="IW735_001158"
     CDS             19686..21170
                     /gene="galT"
                     /locus_tag="IW735_001158"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1044171.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21194..22174
                     /locus_tag="IW735_001159"
     CDS             21194..22174
                     /locus_tag="IW735_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1044172.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22263..23087
                     /gene="tsaB"
                     /locus_tag="IW735_001160"
     CDS             22263..23087
                     /gene="tsaB"
                     /locus_tag="IW735_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1044173.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23071..23613
                     /gene="rimI"
                     /locus_tag="IW735_001161"
     CDS             23071..23613
                     /gene="rimI"
                     /locus_tag="IW735_001161"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1044174.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23620..24084
                     /gene="rimI"
                     /locus_tag="IW735_001162"
     CDS             23620..24084
                     /gene="rimI"
                     /locus_tag="IW735_001162"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1044175.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24081..25097
                     /gene="tsaD"
                     /locus_tag="IW735_001163"
     CDS             24081..25097
                     /gene="tsaD"
                     /locus_tag="IW735_001163"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1044176.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8327 a         4397 c         5376 g         7575 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tattgacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaagaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attattcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaat aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 cacacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcaac caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatct cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt tagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat tttttctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga ttttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaattga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggt aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaaaga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgccta
    17521 tcagaatgag cccttttcag aagctagccg aatatttatt gaagggcaga taaaagaagc
    17581 ggaaaaaggc aattgcacag gagcatttgc ttcaacattt gatgcattaa aagactggcg
    17641 tgatccaatc cggcaggcta ttgaatggca cgttttttct gctaaggaat acaaagaaat
    17701 actatgggga tggtttactc atctaaatag ctttttaacg atcggtccgc caatcattcg
    17761 gacgaaagaa ttagcagcac tgatagatgc gggtattttt catttgatcg aaccgccatt
    17821 tgatattcag caaaagcaca accgctatgt tatttctcta caagataaag aaatcagcag
    17881 tcgctatttg attgaagctc gattgccaca aacaagcttt tctcgttcaa gtaatcctgt
    17941 cataaaaaat ttgaaggaaa acaaacttgt tcgccctttt tcattttcag ataatacagg
    18001 aagttatcat tcagaagcta tagatgttga gcgaggtacg aatcgcgtga ttgatgcttc
    18061 aggggaaatc cagtctgact tttattgcta tggaatacct gttgaaggtg tggattggct
    18121 aactgcagca gtagctagac cttatacaga tccatggaat ctgagacagg cagataaaat
    18181 agcacaactt atattgactg acaaataaaa atacgcatga tgacttatct gttttttaga
    18241 aagtcttcat gcgtattttt aaataattat taacgagaaa cgcgtttacg cattgctttt
    18301 ttacggtttt cttcgatcat tgcttctttt tcgtcaattg gatcgatcac tgtttttttc
    18361 acagaagcaa ccaaaccagc aacggcagcg gctgttgcca gacttcctac taatattcca
    18421 gatacaaatt ttttcattga atccacctca tttattattt gttatatcta ttatggaaca
    18481 aaagagaaaa aaaggctagt tttacgtttg tttgtaaacc tttgcaaaaa atccttgaaa
    18541 atgtttacta aatgttttat tataaattta ccatatgaat ttacgaaatt ttactttgtt
    18601 tgttttcacc ataaaagtaa acttcgtttt ttaaattata tagaatcaac aaacacaatt
    18661 ttactaatca aaggagtgtt ttttatgaca attttagtac taggcggagc cggttatatc
    18721 ggttcgcacg ctgtagacca gctagttcaa aaggggtatc aagtagctgt ggtagataat
    18781 ttgctcacag gtcacaaaca agcagtacat ccagatgctc atttttatga aggagacatc
    18841 cgggataaag aatttctacg ttccgtattt gaaaaggaac cgattgaagg agtaatccat
    18901 tttgctgcaa gctctttagt aggagaatct gtcgaaaaac cattgatgta tttcaacaac
    18961 aacgtatacg gtatgcaaat cttacttgaa gtcatgcacg agttcaacgt gaataaaatc
    19021 gtattctctt ctactgcagc aacttatgga gagccgaaag agtctccaat cacagaagat
    19081 acaccagcta atcctaaaaa tccgtatggt gaaagtaaat tgatgatgga aaaaatgatg
    19141 aagtggtgtg accaagcata tggcatgcgc tatgtcgctt tgcgctactt caatgtagcc
    19201 ggagcaaaag cagatgcatc tatcggagaa gatcatacac cagaaacgca tcttgtccca
    19261 atcattttac aagtagctct tggtcaaaga aaagccttgg ctgtttatgg agacgattat
    19321 gatacaccag atggaacatg tatcagagat tatgttcaag tagaagattt gattgctgca
    19381 catattttag ctttggaata cttgaaagaa ggaaatgaaa gcaacttctt caatttaggc
    19441 agcagcaaag gatactcagt caaggaaatg ctagaagcag ctagagaagt gacaggaaaa
    19501 gaaattccag cagaaatagc accaagaaga gctggagatc ctagccgatt agtcgcttca
    19561 agtgaaaaag cgagagagat tctgggctgg aaacctgaat acacagatat caaagcgatt
    19621 atcaaaacag catgggattg gcatgtcagc catccaaatg gttatgaaga ataggggaat
    19681 gagatatgtc aatcagtcaa acgatttgtg attttgccac acttgcgatc gaagctggcg
    19741 gatggatgga aatggaccgc ttatatttac agaatcgtct tttagggatg gtaggagaag
    19801 atgctctaga aagcattgaa gtaagaccag ttaaggaaga atctgtagac ttattggatc
    19861 agcttgtcgc taaggcaaga gaaaatggtg ttctttcaga attgccagca gaacaagaga
    19921 tttttgaagc gcaattaatg gattttctca caccgccacc ttcagtcgtc aatgcattct
    19981 ttgcacagca ctattcgaaa agcccagagg aagcaactga gtatttcttt aatttaagta
    20041 agaaaaatga ctacatcaaa acaagagcca tcgcaaaaaa catcaaattt ccggcagagt
    20101 cggagtatgg tgaactggaa atcacaatca atttatctaa gccagaaaaa gatccaaaac
    20161 aaatcgcagc agaacgcgag gcggtaaaag tagactatcc tacctgcatg ctttgcatgg
    20221 aaaacgaagg atatagaggt cgcttgaact atccagcccg caccaatcac cggattattc
    20281 gaatgaatct agacggtgaa agctggggat tccaatattc cccttatgct tattacaatg
    20341 aacattcgat cattttatca gaacaacacc gcccgatgaa aatcacaaag gaaacatttg
    20401 ctagactgct tcggattact gaagtcttgc ctcattactt tgttggttcg aatgctgact
    20461 taccaattgt tggaggatcg attctttctc atgatcatta tcaggcagga cgtcatgaat
    20521 ttcctatggc aaaagcaccg atagaaaaac ttatcaaact agaagattat ccgcaagtca
    20581 tcgcaggagt cgtgaaatgg ccgatgtctg tgatccgttt gcaatcaaca gagaaaaact
    20641 tgcttattga agcagctaac tcagtattgg aaaaatggaa agcatattcg gatgaaacag
    20701 tatctatcag agctttttca gcagatggta caccacacca tacgattacg ccaattgcac
    20761 gaagaaggga caattacttt gagttggacc ttgtccttcg agacaacaat gtttcagaag
    20821 aacatcctga tgggattttt catccacata aagatgtcca acatatcaaa aaggagaaca
    20881 ttgggttgat cgaagtaatg gggcttgctg ttttaccacc tcgtttgaaa ccagaactag
    20941 tagaagtaga gcatttctta ttaggagaag aaaaccagat ggctgaatat catcgtccgt
    21001 gggcagaaca attaaaaaaa gaacatcctg atttgacgaa agatacggtc accgatgtcg
    21061 tacaaaaatc tgtaggtcag gtttttgcac gtgtactgga agacgctgga gtattcaagc
    21121 gagatgaaaa aggacaggca gcgtttgaac gatttactgc gattttataa acgtaaaaaa
    21181 ggaggagaga caaatggcaa caattaaaga cattgcgcag ttggcaggag tttctcctgc
    21241 tactgtttca agagttctca actatgatcc tgatctttcc gttggcttag ccacaaagca
    21301 aaaaattttt gaagcagcag aacgcttaaa ttatacgaaa catcgtaaaa atgcagcaaa
    21361 tactaagcaa acgcttcgcc tgatccagtg gtacgacgat acggaagagc ttgaagatct
    21421 ttattattta tctattcgcc taggaatcga aaaaaaggca gaagaagcaa acgttacttt
    21481 attgaaagaa acgtgggatt ccattagtga tcttccggca gatggaacga tcgcactagg
    21541 aaaattcgac gaagctcagt tattagaaat gaaaggatcc caaaatgcct tgcttttggt
    21601 agattctgac ggcacttctc aaggaatcga ctctctagtt gtcgacttta agtcaagtgt
    21661 tcaaaaagtc attgaacact ttgtctccca taaagcgcaa catatcgcta tgcttgctgg
    21721 aacagaatat acgaagaaaa accatcagcg gctaaaagat cccagaatta tggcatttca
    21781 acaagttttg gcagaaaaag gctacgacga aggtccaatt atcgaagcag atttttctgt
    21841 cgagtcagga tatcaagcag tgaagcagta tttaaaaacg aatccgcatg ttcctgatgc
    21901 gctttttgca gctaatgatg cattagcgat tggcgcactg aaagcgattc aagaaaaagg
    21961 tttagtggta ccagaagata tttctgtgat gggcttcaat gatgtcagtg tagcgaaata
    22021 tgtaacgccc tcgctttcta ctgtaaaagt ttataccgaa tggatgggag agctagcagt
    22081 ggagacgatt cttcaattaa gtaattccaa agcaccggtt tcaagaaaga ttattgtgga
    22141 aacagagttg attattcgag aatcaacgaa ataaaagatg gaaaagctgt gggaaaatgg
    22201 agtgataccg cagctttttt gtgtttcagg aaggggcaaa atttgcccgc cagcagataa
    22261 tcatgtatta ttgtactagt tttgcctttg gtattttcat cctcagaata aaaaagtatg
    22321 ctacaatgga aggcgagaat gtcatagcag aaaggatcaa atatatgatt acattaggaa
    22381 tagatactgc aaaccaaact ttggcgatcg gtgtggtaga agacgaacaa attcttggac
    22441 aaatacaaac aaatatcaaa cgcaaccaca gtgtaacact gatgcctgca atcgaccaat
    22501 tgtttgcaga tcttcaaata agtccaaaag acattgaccg aatcgcggtt tctgatggtc
    22561 ccggttctta tacagggctt cgtataggtg taacgacagc aaaaacgatt gcttatacat
    22621 tagataaaga gctagtagga gtatctagtc tgaaaaccat cgcggctaat tgcatcggtg
    22681 tggataatat tattgtgccc atgtttgatg ctcgaagaaa aaatgtatac gctggggcat
    22741 accgctggaa agaaaataga ttagaaactg tacttgaaga tgtacatatt tctgcttctg
    22801 atttgttcac acaactacaa aagctgaatg aagctattta tttcgtcggg agtgattgtc
    22861 acaaattctt ctctgaaata aaggaaatat tgcctagtgc gatgattaat acagttccag
    22921 catgggatat tccaagtggt gtaactgtgg ctcaattagg tgctttagtt gaaccagttg
    22981 cagagaaaca agcttttctt cctcgctatt taaaacgagt agaagcagaa gaaaaatggt
    23041 tggagacgca cacacctgga gacgaaagtt atgttgaaaa aatttagagc gtatgccaga
    23101 agcttttttc aagataaaat gatttataca gaaaaaacag tcgaacaaat cgatcgtcgt
    23161 tacgtactaa gagaattgac tacagaagat atcaaagagc tgctgggtgt cgaacgagaa
    23221 gtctataatg gcgaattgcc atggacaaaa agtgccttta tatcagaatt gaagtcaccg
    23281 cttcctcatc tatatctagg tatctttgat aatggaagaa tgattggttt tatcggttca
    23341 agagcgttcg gtttagattg ccatatcacg aatattgccg ttcgaccggc atatcagcgt
    23401 aaacgaatcg gttcgctgct tattgacgaa atcgagaact ttgctataat gaatcgctgt
    23461 gagacgatgt cattagaagt gcgtatgagt aatcaagatg cacaaagact ttatcggaaa
    23521 ttggggtttc aagcaacaac gatcaaaaaa agatattaca ctgaaacaaa agaagatgcg
    23581 ttggatatga taaaactttt agaaaatgag tgagattagt tgatttgcag aaaaaaagat
    23641 tttgatgaag cgtacttggc agaatgcgta tggatgatta gcgaaaatgc ttatccacat
    23701 ggttcacctt ggacgaaaat gcagtttttg gcagacatcc aacagcctca ctctgattat
    23761 ctgcttttag ctgaagataa ccagatctgt ggctttattg gatactcgaa agtaatagat
    23821 gaagtcgaga tcacaaatat tgctgttgca gtttcagaac aaggaaaagg gcatgcacgg
    23881 catttgttac aattactgat tacggaacaa ataaaagatt cgctgcatgt ttttttagaa
    23941 gtacgtctat ctaatgaagc agcaagaaaa ctttatgaat cagaaaagtt tcgtatatta
    24001 ggtaaacgga aatcatatta tcgcaatccg acggaagatg cgatcattat gagcagaaag
    24061 ttaaaaacgg agacgatgaa atgaatgaag aattgattct ggcgattgaa tcaagttgtg
    24121 atgaaacgag tgtagcggtc gttagaaatg gaacagagat cttatctaac atcgttgctt
    24181 ctcaaataaa cagtcataaa cgttttggtg gcgtagtacc tgaagtagct agccgccatc
    24241 acgtagaaca aatcacctta tgcttggaag atgcactggt agaagctggt gtgtcggcag
    24301 aagatttatc ggcagtcgct gtcacttatg gaccaggact tgtcggctcg ttgctgattg
    24361 gtatttctgc agctaaggct tttgcttggg cacaccaatt gccgcttatt cctgtaaacc
    24421 atatggcagg gcatatctat gcagcccgtc tggttaaacc attccagttt ccattgatgg
    24481 cacttcttgt tagcggagga catacagaat tagtctatat gcaagaagat ggatcatatg
    24541 aaatcattgg ggagacaaga gatgacgcag caggagaagc ttatgacaaa gttggaagag
    24601 tattaggtct ttcttaccca agcggaaaag aaatcgatca gctagctcat cagggaaaag
    24661 ataattacca ttttccgcga gcgatgatcc atgaagataa ttatgatttc agttttagcg
    24721 gattaaaaag tgcatttatc aatttggtgc ataatgcaca acagcgtgga gaagatctag
    24781 acaaaaatga tttagcagct agctttcaag caagtgtgat cgatgtattg attactaaaa
    24841 cacttcgagc ttgtcaaaat tatccagtca agcaactgat tatagctgga ggtgtagcgg
    24901 ccaatcaagg gttgcgtgaa ggattgcagg atgctttggc aactaaactc cctgaggtag
    24961 aactagtgat tccccctctt cgactttgtg gagataatgc tgcaatgatt ggagcagcag
    25021 cacatgtgga aatgcaaaaa agaaattttg ctagctatca gttgaatgct gatccaagct
    25081 tggttttatc tgaataaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
    25141 cccaggaaaa acggcttctc atgtttttga cctggctgtt cttattaccg cgtatgcaag
    25201 cttttgaacg ccgtttaatc aagagttcgt aagcctgtcg tttaactctg attcacaagg
    25261 aacaatttca aaaaacagct cctcatgttt atgaacatta tgacttgtga ccgaagagtt
    25321 gacaggtaca caccgtttaa tcgaaatagg gctgtgacac gtttttgtca aagccctatt
    25381 ttttacatac ttttaatcat tattgttatt ttgctatttt ttacagctta tttatcatta
    25441 tccgttcctc tgattgccct cactgagtaa tcgtttattg tcgaggaata tcgaataatc
    25501 tagatcggat gaggacaaga cgcttcatct agttaatcta acagtatttt ttgcaaacag
    25561 tgaatattac ataactgaaa cacacataga aaatctttga gtcttgtcca gtgatctaaa
    25621 actgccggat ctttcagaag ttttagggag gaaaataagc agtgaccctg atatt
//
LOCUS       DACEMS010000032        25516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEMS010000032 DACEMS010000000
VERSION     DACEMS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.032"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="IW735_001164"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="IW735_001164"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1044177.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="IW735_001165"
     CDS             complement(860..1678)
                     /locus_tag="IW735_001165"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1044178.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IW735_001166"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IW735_001166"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1044179.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="IW735_001167"
     CDS             complement(2967..3923)
                     /locus_tag="IW735_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044180.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="IW735_001168"
     CDS             complement(3923..5056)
                     /locus_tag="IW735_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044181.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="IW735_001169"
     CDS             complement(5049..6611)
                     /locus_tag="IW735_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044182.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="IW735_001170"
     CDS             complement(6823..7905)
                     /locus_tag="IW735_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1044183.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="IW735_001171"
     CDS             complement(7971..8369)
                     /locus_tag="IW735_001171"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1044184.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IW735_001172"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IW735_001172"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1044185.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="IW735_001173"
     CDS             complement(9098..10399)
                     /locus_tag="IW735_001173"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1044186.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="IW735_001174"
     CDS             complement(10540..11556)
                     /locus_tag="IW735_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1044187.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="IW735_001175"
     CDS             complement(11864..12469)
                     /locus_tag="IW735_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1044188.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="IW735_001176"
     CDS             12665..13588
                     /locus_tag="IW735_001176"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1044189.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="IW735_001177"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="IW735_001177"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1044190.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15703..17334)
                     /locus_tag="IW735_001178"
     CDS             complement(15703..17334)
                     /locus_tag="IW735_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1044191.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17348..19021)
                     /locus_tag="IW735_001179"
     CDS             complement(17348..19021)
                     /locus_tag="IW735_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1044192.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19034..19351)
                     /locus_tag="IW735_001180"
     CDS             complement(19034..19351)
                     /locus_tag="IW735_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1044193.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IW735_001181"
     CDS             complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IW735_001181"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1044194.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21013..21798
                     /locus_tag="IW735_001182"
     CDS             21013..21798
                     /locus_tag="IW735_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1044195.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21801..22493
                     /locus_tag="IW735_001183"
     CDS             21801..22493
                     /locus_tag="IW735_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1044196.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22767..23138)
                     /locus_tag="IW735_001184"
     CDS             complement(22767..23138)
                     /locus_tag="IW735_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1044197.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23538..23768)
                     /locus_tag="IW735_001185"
     CDS             complement(23538..23768)
                     /locus_tag="IW735_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044198.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23862..24200)
                     /locus_tag="IW735_001186"
     CDS             complement(23862..24200)
                     /locus_tag="IW735_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044199.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(25128..25244)
                     /locus_tag="IW735_001187"
     CDS             complement(25128..25244)
                     /locus_tag="IW735_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1044200.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         7435 a         5604 c         4499 g         7978 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aacgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcactg attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggcccctagt ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagc agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatcca ttaggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aagtatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaacaat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcaa tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat aaagagccaa cataatatgg agggggaatc
    15241 cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt
    15301 tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag
    15361 ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca
    15421 tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa
    15481 ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca
    15541 aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct
    15601 ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg
    15661 gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga
    15721 aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca
    15781 aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac
    15841 aaccgcagta tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa
    15901 ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac
    15961 attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc
    16021 aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt
    16081 ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag
    16141 atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa
    16201 atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt
    16261 catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa
    16321 gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata
    16381 ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa
    16441 agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat
    16501 cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga
    16561 accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt
    16621 gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat
    16681 tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc
    16741 agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat
    16801 tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat
    16861 ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag
    16921 accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg
    16981 aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt
    17041 ttccagatca tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca
    17101 caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga
    17161 aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc
    17221 tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc
    17281 ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc
    17341 tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta
    17401 atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt
    17461 ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct
    17521 gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt
    17581 cagatgcacc tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa
    17641 gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta
    17701 cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg
    17761 cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt
    17821 caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa
    17881 ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca
    17941 atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa
    18001 taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga
    18061 aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga
    18121 gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat
    18181 tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat
    18241 tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat
    18301 gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc
    18361 cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt
    18421 ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag
    18481 gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg
    18541 taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg
    18601 aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga
    18661 cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt
    18721 tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa
    18781 cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag
    18841 attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg
    18901 aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat
    18961 tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca
    19021 tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat
    19081 ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct
    19141 ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg
    19201 ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta
    19261 cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa
    19321 aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa
    19381 taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct
    19441 tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg
    19501 agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg
    19561 ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa
    19621 tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt
    19681 aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca
    19741 gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca
    19801 ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat
    19861 cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg
    19921 atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt
    19981 cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat
    20041 ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg
    20101 cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt
    20161 cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg
    20221 cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat
    20281 tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat
    20341 gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc
    20401 catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt
    20461 ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt
    20521 tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac
    20581 caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat
    20641 cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac
    20701 cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt
    20761 ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa
    20821 ctgattgcat tgtaaacgca tccgaaacat ttgtcaacaa aaacaaacac aaataatcat
    20881 aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt
    20941 tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta
    21001 gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa
    21061 aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt
    21121 cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct
    21181 caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag
    21241 attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg
    21301 attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa
    21361 attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca
    21421 gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc
    21481 accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt
    21541 cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta
    21601 aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt
    21661 tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg
    21721 gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat
    21781 accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt
    21841 tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg
    21901 gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga
    21961 aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag
    22021 aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc
    22081 ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta
    22141 ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact
    22201 gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc
    22261 ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt
    22321 actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat
    22381 tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag
    22441 attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc
    22501 aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt
    22561 gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac
    22621 tttttcattc ttttaaccat atgacgcgtt catctctatc tataattaac attaaaaata
    22681 gtgttttcac attatttctc aaaccaatct taaaaatcat caggagtact tttttgatat
    22741 acttttatta atgctgggcg taatggttaa aagatttcca ctagttcctc tccaccaagc
    22801 tgagttgaaa aatgcttatc tgtaaacaac atagacaaag aggaaataaa cgtttccgta
    22861 tctttctctg tcccaaggat attaattaat tttcctacta ggtaagtatc attaaactta
    22921 agatctgcct tcacatcaaa agttttgata aaagctttta tcatgcgtac aattttgtct
    22981 ttatcgccat aattgatttc accactcata tcaatatagt cactaatccc tgaagaaata
    23041 atggcaactg cttcttcttt cgccttatca gataattcat ttaatgactg ggattgattg
    23101 atgacttctt gagttttatc tgttacgtaa tctctcatta tcctatcggc atactccgca
    23161 aagtaatctt gaaaaaattc ttctacaggg taaccaactt cgttttccca accaggagaa
    23221 atttcattat cagtacttat taaatctatt aaaaaccaac ccgttctctg agtgttgatt
    23281 ctataatatt tttaatttcc tatataaatc aaaaatatta tagaatcaat aagctaatta
    23341 agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt gtgtagctac
    23401 ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac tgcattaaag
    23461 ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg tcaaaatttt
    23521 tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc ttctacttca
    23581 gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga tccttctctg
    23641 taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc tttccactca
    23701 ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt tcccttttct
    23761 accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta catctagtaa
    23821 attttcatca atttctatat cataaagtat ttcactagtg tctacatcca caatatatac
    23881 tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc gtcactttca
    23941 tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag ctctaagcct
    24001 actgtatttt ttactattga gtcattattt accaccataa cttcgtactg tatataccat
    24061 cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt ctccggaaca
    24121 atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc atgttcctca
    24181 cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa aataaacact
    24241 acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc caatcacaaa
    24301 ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac tacaactatt
    24361 catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga tacttgtaat
    24421 agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa ttccaataca
    24481 caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt attctagcaa
    24541 tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta aagttaactt
    24601 tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga aatggagtga
    24661 gctgaacgtt agagatattg ggagctttcg taactgctta ttaaacgata atggttattt
    24721 gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa atatttcgca aaacctttga
    24781 ttatgtagtt acgtttccat tcttgactaa aaatgtttca aaaacgaaca aaggtgttaa
    24841 aaggtaaagt gatcaatact actggtcaaa ataagaggtt gaaagggaat tgaaaaataa
    24901 ctacagataa tacttttata aacgtacgtg ctttgtggtt ctgtagttaa ctatataact
    24961 ggaattaggg aatggtatca atttgaaaag aaagattcta ccaatttatc atatgttaaa
    25021 aatggaaaat caacagcagt tctttcggaa accttacaca aaaaccgatt attgaaaata
    25081 gccgaaaaac gtttttcggc tattttttta attaagtttc cttcttctta ccttttccca
    25141 ctattccgtt tcacatcttt gatgatcttc agcgcacgtt ttcgtgtttt gatatttggg
    25201 cttttcaagc ggcggtatgc acttgagata tctgtttttt ccatatggac gacccacttt
    25261 cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa caattacgat ttgtgtttta
    25321 aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa cagtcgatca gactagctga
    25381 ttgactccta tctctggaaa taaattttca ttattacgtt ttagctacct aaaaaaggcc
    25441 ctcttgtccg ttaactttaa tatctattgt tatagctagt ttacctattg aacgtactgt
    25501 gtgttcgata gaacac
//
LOCUS       DACEMS010000033        25379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEMS010000033 DACEMS010000000
VERSION     DACEMS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.033"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(153..530)
                     /locus_tag="IW735_001188"
     CDS             complement(153..530)
                     /locus_tag="IW735_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044201.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            786..1514
                     /locus_tag="IW735_001189"
     CDS             786..1514
                     /locus_tag="IW735_001189"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1044202.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1514..1768
                     /gene="purS"
                     /locus_tag="IW735_001190"
     CDS             1514..1768
                     /gene="purS"
                     /locus_tag="IW735_001190"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1044203.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1770..2441
                     /gene="purQ"
                     /locus_tag="IW735_001191"
     CDS             1770..2441
                     /gene="purQ"
                     /locus_tag="IW735_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1044204.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2442..4664
                     /gene="purL"
                     /locus_tag="IW735_001192"
     CDS             2442..4664
                     /gene="purL"
                     /locus_tag="IW735_001192"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1044205.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4649..6088
                     /locus_tag="IW735_001193"
     CDS             4649..6088
                     /locus_tag="IW735_001193"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1044206.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6120..7163
                     /locus_tag="IW735_001194"
     CDS             6120..7163
                     /locus_tag="IW735_001194"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1044207.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7160..7738
                     /gene="purN"
                     /locus_tag="IW735_001195"
     CDS             7160..7738
                     /gene="purN"
                     /locus_tag="IW735_001195"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1044208.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7735..9270
                     /gene="purH"
                     /locus_tag="IW735_001196"
     CDS             7735..9270
                     /gene="purH"
                     /locus_tag="IW735_001196"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1044209.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9286..10560
                     /gene="purD"
                     /locus_tag="IW735_001197"
     CDS             9286..10560
                     /gene="purD"
                     /locus_tag="IW735_001197"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1044210.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10682..11596
                     /locus_tag="IW735_001198"
     CDS             10682..11596
                     /locus_tag="IW735_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1044211.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11729..13294)
                     /locus_tag="IW735_001199"
     CDS             complement(11729..13294)
                     /locus_tag="IW735_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1044212.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13287..14738)
                     /locus_tag="IW735_001200"
     CDS             complement(13287..14738)
                     /locus_tag="IW735_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1044213.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTIEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14752..15768)
                     /locus_tag="IW735_001201"
     CDS             complement(14752..15768)
                     /locus_tag="IW735_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1044214.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15988..17076
                     /locus_tag="IW735_001202"
     CDS             15988..17076
                     /locus_tag="IW735_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1044215.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17130..18656
                     /locus_tag="IW735_001203"
     CDS             17130..18656
                     /locus_tag="IW735_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044216.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18658..19863
                     /locus_tag="IW735_001204"
     CDS             18658..19863
                     /locus_tag="IW735_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1044217.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20120..21097
                     /gene="guaC"
                     /locus_tag="IW735_001205"
     CDS             20120..21097
                     /gene="guaC"
                     /locus_tag="IW735_001205"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1044218.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21361..22785
                     /locus_tag="IW735_001206"
     CDS             21361..22785
                     /locus_tag="IW735_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1044219.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23246..24355
                     /gene="pdhA"
                     /locus_tag="IW735_001207"
     CDS             23246..24355
                     /gene="pdhA"
                     /locus_tag="IW735_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1044220.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24358..25335
                     /locus_tag="IW735_001208"
     CDS             24358..25335
                     /locus_tag="IW735_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1044221.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8217 a         4317 c         5698 g         7147 t
ORIGIN      
        1 aaatagaata gcaaaaataa ccaaaaaaga agcagatccg ttagcggttc tgcttctttt
       61 agtttattat attttcttat caataatagg aacctgattt taaaagcggc tacctcttta
      121 ccgtgcctct ttacatacaa aaaatagagg aattaagcgt aaatggcttg gctattataa
      181 ctaaaatgct ggttatggag atgttgcagc aagttttcga gaaaagcgta ttccatctct
      241 tgtccattga ataagatctg gatacgataa tgatttttga aacgcttctg ttcatgaatc
      301 tgactgattg aaagttcgcc tttgtgttct gtctgataaa gaaaagtcct tttacggcat
      361 tcagcacagc gcttggttgc tcggcggatt tttcggaatg tataaaaact agtatcttta
      421 ttgcataaat aaacgtcttt gatcgacagc aaatcattat atagacagga acgttcttca
      481 gctgttaatt ctgataaaaa aagctgacct tgttttattg catcttccat cggcaagtgc
      541 ctcctcgaac ccattatcca aatttttagt taattctaat ataccatcat taagtataaa
      601 tgtaaacgct tgttgatggt ataagatttt cgtttctgat aaaacacgaa cattaaaaga
      661 atctaatgcc ttaaatgtac atatttttgt tgatttataa aaagatgatt gctaaaattt
      721 aaagtgaaaa aacgaacaaa aaacgatgta tttgctttta atgtaaataa aaaggagaga
      781 cgtacatgga gaagaaagaa cttttgtatg aaggaaaagc caaaaaaata tatcttactt
      841 ctgatgagaa cttgatttgg atagaatatt tagatcaagc aactgcgttg aatggcgcga
      901 ggaaagacca gataagtgga aaaggagaat tgaataatca aatcacaagt gcaattttcc
      961 gttatctgac tcaaagagga ataaaaaatc attttattga acaattgtct aaaaccgaac
     1021 aattagcaaa aaaagtacaa attatccctc tggaagtggt tatccgaaat attgcagcag
     1081 gaagtttctc aaaacgtcta ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg
     1141 aattctacta taaggatgac cagttggatg atccatttat caatgaagag catatctatt
     1201 tcttacaact tgcaaagccg gaagaaatca gtgagctgaa agcccaagcg ttggctatta
     1261 atgaaatttt gaccgaatta tttgctaagg tcggtttggt tttagtagat ttcaagctag
     1321 aatttggccg gtcgaatgga caaattttgc ttgcggatga agtaacaccg gatacttgcc
     1381 gattatggga tgaacgtacg aatgaacata tggacaaaga cgtttaccgc agagatctag
     1441 gtgatttgat cccagtctat caagaagtat ataaccgtct atcggaacta actgaagaaa
     1501 aggagcgagt atgatgtatt tcgtaaaagt atatgtaacc tataaagatt cagtgttaga
     1561 tcctcaaggg gaggcagtaa aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga
     1621 tatccgaata ggaaaatact ttgagataca acttgctgat gcagaagaac caatcgatac
     1681 agtgattgaa acgatttgtg acaagctgct tgcaaatgta aatatggaaa cataccgata
     1741 tgaaatcaaa aaaatggagg aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt
     1801 caaattgtga cgcagatatg ttatgggcag ttcgcgagat catgggcgct gatgccgaat
     1861 ttgtacggca tgatgagtcc tctttagaag gatttgatgg ggttttactg ccgggaggat
     1921 tctcctatgg tgactatttg agatgcggtg cgattgctcg gttttctaca atcatggaag
     1981 aagtgattcg ttttgctgaa gaagggaaac cagtattcgg tacatgcaat ggattccaga
     2041 tattgacaga agcaggactt ttgccaggtg tactgagacg aaatgagtct ttgcatttta
     2101 tttgcaaaac tgtaccattg aaagtcaacc ccaagacggc ctttacttca gagtatgaag
     2161 ccgacgaaat aatctatctt ccagttgctc atggggaagg aaattatttc tgtgacgaag
     2221 aaacacttca agaactaaaa aaaaataatc agattgcctt tacttataat gacaacatca
     2281 acggcagcgt ggaaaacatt gcagggatta cgaacaaagc agggaatgtg ttaggaatga
     2341 tgcctcatcc agaacgtgca gtagaagact tgctaggatc agaagatggg aaacggttct
     2401 ttgcttccat tttaaaaaac ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac
     2461 cgacagctga agagatcaag aaaaaaagaa tctatgccga atggggattg actgatgaag
     2521 aatatcgaat gatcgaagaa gatatcttag gaagaatgcc taattatacg gaaactggac
     2581 tgttttctgt tatgtggagc gaacattgtt cctataaaaa ttctaaaccg gttctacgca
     2641 aatttcctac tagtggacct catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg
     2701 acatcggtga tggactagca gttgtcttca aagcagaaag tcacaatcat ccttcagccg
     2761 tggagcctta tgaaggagct gcgacaggag ttggcggaat catcagagat attttcagca
     2821 tgggggctcg tccaattgcg ttgctagatt cattgaggtt cggcgaatta gataatccaa
     2881 gaacgaaata tttactagaa gaagtagttg caggtatcgg cggatacggc aattgtatcg
     2941 gcataccgac tgtaggcggc gaaattgcat ttgatccttg ttatgaagga aacccattag
     3001 tcaatgcaat gtgcgtaggt ttgattgaac acaaacatat ccagaaaggg caagccgctg
     3061 gcgtcggtaa ttcgatcatg tatgtaggtg caaaaacagg tcgcgacggt atccacggag
     3121 caacttttgc ctcagaagaa tttaatcaag aagaagaaca gcaacgttct gctgtacaag
     3181 taggcgatcc ttttatggaa aagctgctgc ttgaagcgtg tttggaatta atcttgcatc
     3241 attcggattc tctaataggt atacaggata tgggagcagc aggacttgtt tcatctagtg
     3301 cggaaatggc ttcaaaagct ggctcaggac ttatattgac attagatgaa gtcccacaaa
     3361 gagaaactgg gatgacacct tatgaaatga tgttatcgga atcacaagaa cggatgttga
     3421 tttgtgttaa atcaggagag gaagaaaaaa tccaagaact atttcagaag tatgatctag
     3481 atgcagtcac tatcggtaaa gtaacagacg acgggcaata tcggctatat catcaaggag
     3541 aagaagtagc aaatctgcca gtcgatgcat tagctgaaga tgcaccagtt tatcataaag
     3601 aaatgaaaga accagctcga atcgtagaat ttcagcagtt agctccttat cagccagtta
     3661 tcgaagaacc tggacaagta ttgcttgatc tgctgcagca accgacgatt gcttcaaaac
     3721 gttcaatcta cgaaacttat gattcacaag tacaaaccaa tacagttgtc cgtccaggaa
     3781 gcgatgctgc tgtgatgagg gttagaggga cgaataaagc gttagctatg accacagact
     3841 gcaacgcgcg ctatctgtat ttggatccaa aaatcggagg acaaatagca gtggcagaag
     3901 ctgctagaaa tattattgct agcggtggta agcccttggc gatcaccgac tgcctgaact
     3961 atggttctcc tgataaacca gaagtttttt gggaattatc gacttcagct gacggaattg
     4021 ctgctgcgtg tgaaacgtta ggaactcctg ttatttcagg aaatgtctct ttatataatg
     4081 aaacggatgg tcaagcaatc tatccaacac caatgattgg tatggtcgga ttgattgagg
     4141 atcataaaca tatcactact caggaattca aaaaatcagg ggatctaatc tatatattag
     4201 gtaagacgtt tgcagatttt gatggcagtg aattacaaaa gatgcagctt ggaagaatcg
     4261 aaggtgtgat ccgaaacttt gatctatcca ttgaaaagag aaaccaagag ttagtattga
     4321 cagccatcca aaacggattg attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa
     4381 ttgcactggc agaatcagct ttcaaacatc agctagggat atctgtacag tttgaattgt
     4441 cctctgctca attatttgca gaaactcaat caagatttgt gttgacagtc gcaccggaaa
     4501 ataagacacg attcgaagaa atgatgggag atgctgctgt tttagcaggt aaggtgacgg
     4561 atgaagcgat aatcgaaatt tcagcaactg acggtcagat caaaatagaa actgcagtag
     4621 caagaaagtg ctgggaggat gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga
     4681 atgcgggatc ttcggtgttt ggggtcatcc agacgccgct agtgtggcat acttcgggct
     4741 ccatagtctt cagcatcgag gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa
     4801 gcttaagggg catcgagatt tggggctgct ttctgaggta ttcaaagatc aaagaaaatt
     4861 gcagcagtta acaggagaag ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg
     4921 aagtgtggac aatattcagc cttttctatt caaatttcat gatcagtcga tcggcttagc
     4981 gcataatggt aacttgatca atgctcgttc ccttagaaaa gagttagagg aaaatggggc
     5041 gatcttccat tctaactccg atacggagat attgatgcat ttgatccgac gaagtgaaga
     5101 aacagatttt ttaaataaat taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta
     5161 tttattaatg acagaaacgg caatgattgc cgctttggac ccaaatggtt ttcgtccatt
     5221 agcaatcgga caaatgaaaa acggagcata cgtgattgcc tcggaaactt gtgcattaga
     5281 agttattggg gcacgtttta taagagatgt ccagcctggt gagatagtga tcatcaatga
     5341 cgaaggaatc cagattgatt gctttacgaa ggacacgcag ctttctattt gttcgatgga
     5401 atatatctat tttgccagac cagattcaaa catcgcaggg atcaatgtgc atactgcacg
     5461 gaaaaatatg ggacggaatt tagcgaaaga atcgccagta aaagcagata tggtcgttgg
     5521 cgtacctaat tcttctttgt cagccgcaag cggttatgcg gaagctagtg gaatccctta
     5581 tgaaatcgga ttagtaaaaa atcaatacat tgcccgaaca tttatccagc caacgcctga
     5641 gctaagagag caaggtgttc gaatgaaatt atctgctgta cgaggagtag tggaaggtaa
     5701 aaaggtgatt atggtcgatg attcaattgt tcggggtacg acaagcagac ggatcgtcca
     5761 tcttttgaaa gaagcagggg caaaagaagt ccatgtgagg attgcttctc ctcctttgaa
     5821 atatccatgt ttttacggga ttgatattca aacgcgtaaa gaactcattg cggcaaatca
     5881 cacgattgat gaaatcaaag aatgtatcgg tgcagattct ttaggttttt taagtgaaga
     5941 gggtctgatc gaatcgatcg gtctggaaaa agatgctccg tattcaggat tgtgtatggc
     6001 ttatttcaac ggagattatc cgacgccgtt atatgactat gaagaaaaat atcaggaatc
     6061 actaaaagaa aaagtatcgt tcttttaaaa gcagaccaag aagggagtat atgaattcaa
     6121 tggcaaatgc atacgcaaaa gctggcgtag atgtcgaagc tggatatgaa gtagtagaac
     6181 ggatcaagaa gcatgtcaaa aaaacggaac gtttaggtat gatgggcgct ataggaggat
     6241 tcggcggttg ttttgattta tcggtctttc aattaaaaga gccagtcctt gtctcgggga
     6301 ccgatggcgt ggggacgaaa ttagcagtgg caatcgaagc agacaaacac gatacaatcg
     6361 ggatcgactg cgtagcgatg tgtgtcaatg atatcttagc acaaggcgca gagccgttat
     6421 atttcttaga ctacttagca acggggaaaa atgatccgtt acgtttggaa aatgtagtaa
     6481 aaggtgtagc agatggatgt gttcaaggaa atatggcgct gattggcgga gaaacagctg
     6541 aaatgcctgg tatgtactca ggtaatgatt atgatctagc cggatttgct gtagggatag
     6601 ctgaaaaatc tcaattattg acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa
     6661 tagcatctag cgggattcat tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc
     6721 agcagcagtt ttctttatca tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat
     6781 tgcttgagcc aacgaaaatc tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa
     6841 taaaaggagc tgcacatatc actggtggag ggtttgtaga aaatatcccg cgaatgttac
     6901 cagaatctat ggctgcacaa atcaaacttg gctcatggcc gattcttcca atattcggct
     6961 gtttacaaaa atatggtgaa attcctgaga tggagatgta tgaaatcttc aatatgggca
     7021 tcggtatggt cctggcagtc gaagaatcga aagtaaaaga aatagtacac gaaattgagc
     7081 aagctggcga aaaagtttat gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg
     7141 tgtttatagg agaagaacga tgagaatcgc tgtttttgct tctggaaacg gaagcaactt
     7201 tcaagcgcta gccgattatc tatcgaaaaa aggattggaa tcttcgattg actggttgtt
     7261 ttgtgatcag cctgaagcat atgttttgaa acgtgcaact gcgttgagtg taccggcaga
     7321 ttgtttttca cctaaagaat ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa
     7381 attgaaagaa aaaaagatcg atttgatcgt attagctgga tatatgcgaa tcattggacc
     7441 ggtattgctg aaaaattatg ataaaagaat catcaatatc catccgtctc ttttacctgc
     7501 cttccctggt ttgcatggca tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt
     7561 cacaattcat tatatcgacc aaggtgtaga tactggacca atcatccggc aggaaaaagt
     7621 gagaattgaa caggaggaca cgttcgattc tttagaagaa aaaatccata gagtagagca
     7681 tcgaatctat ccagaagtga ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca
     7741 agagcattga tcagtgtatc agataaaact gggattgtag cattcgccca aggtttgatt
     7801 gaacaaggag tagaaatcat ttcaacagga ggaacaaagc atgcgttgga taaagctggg
     7861 atcaaaacgc tttcgatcga agaagtgaca gggtttcctg aaatgatgga tggccgtgtc
     7921 aaaacacttc atcctaaaat ccatggtggt cttttaggaa gacgcgatct ccctagccat
     7981 atggaggcaa tggaaacgga aaatatccaa ccaatcgact ttgtttgtgt caatttatat
     8041 cctttcaaag aaacgattat gaaaccagat gtcgaaatag cagaagctat tgaaaacatt
     8101 gatatcggtg gtcctagtat gctccgtagt gcagcaaaaa accatgaatt tgtcacctct
     8161 gttgttgatc catctgacta tgaagaagta ctttcagaat tacgtcagta tgggcatact
     8221 tcactttctt tcagacaaaa actagcggca aaagtctttc gtcatactgc ggcatatgat
     8281 gcactgatcg ctcaatactt aacggattgg gtggaagaaa aagaaccgga aaaattgaca
     8341 cttacgtatg aacgaaaaca aacgcttcgt tacggtgaaa atagccatca agccgctgcc
     8401 ttttatcagt ccgctcttct ggaaaaatat tcgatcgcag cagcaaaaca actccatggg
     8461 aaagaacttt cttataataa tatccgagat gcagatgctg cgctacggat catgaaggaa
     8521 tatgacgagc caacggtagt cgccttgaaa cacatgaatc cttgtggtat tgggacagca
     8581 tctacgattt tagaagcgtg ggaagcagcg tatgaagcag atcctgtttc aatctttggt
     8641 gggatcatcg ttttgaatcg agaagtagat ctgccaacag cagaacagat gcataagctg
     8701 tttctagaaa tcatcattgc cccttcttat acacaagaag ctttggaatt actgcaaaaa
     8761 aagaaaaaca tccgattact gacagtagac tttgcgcagg aagaaaatca aaatcgagaa
     8821 gaaacggtct cagtcttagg tggacttctt gtacaagatc aagatctggc aatagaaaat
     8881 gaagaagagt ggaaagtcgt gacaaaacgt aagccgactc cagcagaagc aaaagcatta
     8941 gtgtttgctt ggagagctgt caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa
     9001 cgtcagacag taggaatcgg agccgggcag atgaatcgag tcggttcagt aaaaattgcg
     9061 attgaacaag cgcaagagaa aatggaaggt gcagttttag ctagcgatgc ttattttcca
     9121 atggatgatt cggttgaata tgcagccaaa catggtataa aagcaatcat tcagccagga
     9181 ggaagcatca aagaccaaga ttctattgat atggcgaata aatacgatct agcaatggtc
     9241 tttactgatg ttcggcattt tagacattag agaggagtcg gataaatgaa cattttggtg
     9301 atcggtgcag gtggtcgtga acatgcaata agtaaaaaac ttttagccag tccgaaggtg
     9361 gatacggtgt attgtgcgcc aggaaatccg gggatgacaa aagatggtat caattgtgtc
     9421 gctatcagtg agcgagatca ttcagcgtta atccattttg ttaagaaaga ggctattgat
     9481 tggagtatcg ttggtcctga agttcctttg ttaaatggga tcgtcgatga ttttatggcg
     9541 gaaggattga aaatcttcgg tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt
     9601 tttgctaaac aattaatgga tacttatcaa atcccgacag ctaaatccag aagttttgaa
     9661 tgttacgaaa aagcagcagc gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg
     9721 gacggcttag cagctgggaa aggtgtagtc gtggctacca cagtgaagga agcgctggat
     9781 gcggcagaac aaatgttgaa ccagcatcga tttggggata gtagcaaaaa aatcgtcgta
     9841 gaagagtttt tggcaggaga agaattctcc cttttggcgt ttgtccgtaa taccaaggtg
     9901 tatccgatgg tcatttcgca agatcataag cgggcatttg ataacgataa agggccaaat
     9961 acaggcggaa tgggtgctta ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa
    10021 gctgtggaag aagttttaaa accagcggct ttgggaatga aagaactagg acgctctttc
    10081 acgggtgttt tatatgcggg tttgattgct acgaaggaag gaccaaaagt gatcgaattc
    10141 aatgcgcgtt tcggtgatcc tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt
    10201 gcgcaagtaa tcgatgattt attagagaat cgtacagtag agttaaagtg gcagcaagaa
    10261 ggatacaatg taggcgttgt ggttgcagct gccggttacc ccgaagaata tgagacagga
    10321 atggttctac cagattttct ggaagaagag ctttctgttt actatgctgg agtttcctca
    10381 aataacggca aactagttgg agcaggagga cgtctttatt tagtagaagc atacggtgag
    10441 acgatcaaag aagcacagaa aaaaatttac accgcgttaa tggataaaac gacagatgga
    10501 acattttatc gtacagatat tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa
    10561 atcagctgct tccatttata aaaaatacaa caaacataat cggaagcgcc gattttagtg
    10621 aagccttttc ataaagagtg tggtacactt ttgttgtgta tataaataag gaggagttca
    10681 gatgaaaaaa attcaattag gtacttctga tcagaaagca gctgcagtga tcttaggctg
    10741 tatgcgtatc aacaatgcgg aagatccagt aaaagtgatt gagacagctt atgaaaacgg
    10801 cattgatttt tttgaccatg cagatattta tggaaacggt gaatgtgaag aaatctttgc
    10861 agatgctcta gcaaaaacat caatcaaacg agaagatctt tttattcaaa caaaatgtgg
    10921 aatcgttcca ggtgtgatgt ttgatttttc taaaaaacat atcattgaat ctgtagaaaa
    10981 aagtttgacc cgtctgaaaa tggattatgt ggatgcttta cttcttcatc gtccagatac
    11041 attggtagaa ccagaggaag tagctgaagc gtttgatgaa ttggaacggt caggaaaagt
    11101 tcgttatttt ggcgtcagca atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt
    11161 tcgtcaacca ttagtagcta atcagctgca atttggcttg aaacatactg gaatgatcga
    11221 tcaagggatt catgtcaata tgacagatga agcaagtatc gatcatgacg gtagtgtact
    11281 tgattattcc cgattgcatg atatgacgat ccaagcatgg tcaccatacc agtatggatt
    11341 ttttgaaggt gtatttatcg ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga
    11401 actagcagct aaatatcaaa cgacaccaac tggcttggct tctgcttgga ttttacgcca
    11461 tccagccaat atgcaagtga tcgccggtac gatgaacgta gggcgaatca aggaaatcgc
    11521 tcaagcttca gaaattaata tgtctcggga agattggtac gaattgtacc gtgctgctgg
    11581 aaacatttta ccttaaagat tgtcataagc aggtcaaaag ggctataaat taaaaagaca
    11641 gaataacttg aaagctgttt tcagcttcga gatattctgt ctttttttat tgcaaatgga
    11701 aagagtatca ttgtacggat ttcgtttatt agttattttt gtttttaagg aaaagctgtg
    11761 tccgatattc ttttggagaa atcccttgtt ttttcttaaa taaataactg aaatagttag
    11821 ggtcattata accaatttca aaagcaatat ctgaaatacg atagtctgat tgctgcaata
    11881 atttttttgc taattcgatt ctttgttctg tcagataatc gatgaatgtt tggttaaacg
    11941 attgagaaaa aatcgtactg aaatgagagg gacttaatgc aacttgttga gcgacccaag
    12001 ttaaggacat atctggatcg gtaaaatttt ctttgataaa atccaatgca tggttcaaaa
    12061 ttggctgata tttgttcctt gaaggctgtg agggcagagc agttaagaaa gaaatgaaca
    12121 tataaactgt cgaacggtaa agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg
    12181 atgtgcaatg aaccatttct tgcgggattt tcttttcttt caggcagaca tgctggagta
    12241 gcttagtcaa gacaaataaa cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg
    12301 tcaagtgttc gatgtactta gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga
    12361 ggtcaaagta aaagacagaa tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc
    12421 tgataatttg ttctttaagg gaactggcgg tttcaagtat catctgcgtg atctcaaaag
    12481 atgcaggtaa ttcgctgatt ctttctgcga caggaccaat ggcgacagaa aaattatttt
    12541 tttgtccctt ttctaattca tggaccaacg tttgggctac ctgataactg gtctcgatca
    12601 cattttcttc aagagaatca actattaaaa atttgatgaa tcgagaggaa acagcagaga
    12661 agatcacttg ttgattctcc ctaaataaaa acttcaaata atcactgaga tgagcgtaat
    12721 cttcaaagtc tgtactgact tggtttgctg ctaataatac ggtcattttt ttccctgcta
    12781 aagagcgatt gaaagattca gcaagtttca atgtttcact gatcgtaagt gagcctttga
    12841 acaaaccgtt caagaaatga ttttttttca attcgaaaac aagatcagaa gaagtgtgcg
    12901 tttctttttg ctgcaaatct aaagcagtta ctgctttttc tattgcttgt ttcaattcgc
    12961 tacttttaat cggtttaagc agataatcat ctgcctgtac ctggattgct cctttcatat
    13021 aatcgaaatc gtcgaatcca ctaataaata tgagacgagt ccaaggaagt agtttccttg
    13081 cttcttttga aaaagtcaac ccgtccatga aaggcatttg gatatcagtc agaatgatgt
    13141 cgggctgtaa gtcgataatt gaagacagtg ccatctcacc atctccagct tcgccgacat
    13201 aagtgattgg ataataagaa ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga
    13261 ggtattcgtc ttcgatgata aaaactttat acatgtttgt ttcctccaat tgttttggga
    13321 gatagaggga tcatcagtgt gacagttgtt ccttctcggt atttgctgtc aatcgtgata
    13381 ggtttttgat catttccgta ataaagaagg agacgcttgt ttacgttgta aagtccgtag
    13441 ccgatactga aatctgaatc gatccctttg gctaactctt tacgtatttc tgccagtttt
    13501 tctgcaggga tgccaatccc gttgtctata actttgaatt ggatcatctc atcaattttg
    13561 catgtggtaa tacgaataaa tcctttgtga cggacgtact tcgttccatg gtatacagca
    13621 ttttcaatca atggctgcaa gatcattttt aaaatagaaa caggaaaaag tgtttctggt
    13681 atatcgatct tatagtcaag catatggccg tatcttattt ttaagatgac tagataatca
    13741 ttgatatggc taatttcttt ttcaagagga atccagtctt gtcctttgct taaagacgta
    13801 cgaaaaaagc cggaaagtgc atgggtcata gtcagagctt cttcaaagca gtcttgttcg
    13861 atcaaagaaa taatggcatc caaactgtta taaataaaat ggggagtaat ctgagcttgc
    13921 aatacacgaa cttcactttg tgctaaatgg tattgtttca aagcgttctc ccgtaaagac
    13981 tggtttagat cttccgccat tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt
    14041 tccggcaggg ttaaacgaaa gccaaggttc cctgaagaaa gttgttctga catctcgatc
    14101 agctgattga tcggttcttg gacctttctc tctaaaaagc gacgattctt tttcgcatag
    14161 aaaaaaatgg tactaagaac tccaagttcc actattgtta atagaatgag cgagcggaca
    14221 agtgtcgtat tttttttgct tgctaagtcg atctccacac gaacaaaatc ttgtaagata
    14281 tctgacagca gttgagtgac agaatctacc tgattgatga tctcttcaat ggtatccata
    14341 aaatcttttg ccaagatatt attgatcagc tcgttttgat aatcttccag tgtatcgatc
    14401 gtacgtagcg atacatctaa cgtactaatt tctttaagtg tatttgtatt atttttgatc
    14461 tctcgaatat cattcctaag ttcttctagg atattttcct cattgaactc atctacggtt
    14521 acttggccag aaatcaagtc ccacatactt tccaaaactt tttgatcaac cttcaaagca
    14581 attgcatctg ctttttggat atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa
    14641 tataacagta tgctcagtag caaagggata acagcaaata gaagcatggc tttgtttgct
    14701 tgggcaaaaa tctgttttat gcttaaattt tttgtcatga tacttctcca gtcaataatt
    14761 tctggttggg atgttagcta tattctgctg ggaaaagaca gtctcggaaa taaaaatatc
    14821 tttaggtaat gtttttcctg tttgaatatt tccaaaataa cgagtaacgg tattggctac
    14881 ataccagccg acatcaggtt tacactcgac tacacaattg acgattcctg agcgtagctt
    14941 ctcgatcata tcttcttgtc catcaatagt tacaatgatc aaatcttctc caggtttgat
    15001 tgatgtttct tttatcgcag ctaaagcacc gtgagtcatt tcatcactat ggctgtataa
    15061 tacatcgatt tcggataaat catttgtttc taaatatcgt ttcatctggt cttttccttt
    15121 catccgaata aaatcaccga caattcggtg aacaatagtc atcggaggtt tatggtcgat
    15181 ggttccatct ttttttaggg ttggtttacg attgattgat tccaaaaatc cttcggagcg
    15241 tagcttggtc ggtgtggagt tctctgaacc agcaagttct aggattctga tttcctgttt
    15301 ctttgaattt gtgaagtgat tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa
    15361 actggggcca atgtgggtga cgaaaagctc gggatcattc gtttgaatat gtctgtcgac
    15421 aagtatgaca gggatccctg ctcgctttgc ttcttttaat acagagtccc acccggattc
    15481 ttgcaaagga gtaaaaatga tcatatccac tttataggca atgaacgtcc gtatatcttg
    15541 gatttgccgt tcctgattca tgtagccatt tcggtagaga acttggtaat cttctttttc
    15601 aagcgcagtg ataattgatt cagtatgctt tttccgccag ctactctccg ttcctgattg
    15661 cgaaaaacca acgactagtg ggtcaggtgc ttcctttgta ttaggtgtac accccatcat
    15721 ttggtaagcg attacaacaa gggctaaaaa aaataaaaca cgtttcactt taaatcactc
    15781 catctggatt tgttagtact atcttatcag ataaaaggga aaatcaaagt caaaaggaat
    15841 caatcgaaaa aaatccaaga aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa
    15901 atttaaagta aatcccataa aaaaatcaat gttaacgctt tctttattag ctatactcgg
    15961 tgtatcaatt gaaatggggg ataaaaaatg aaaaacaaat ttagaaagat tgttgggact
    16021 atcgctattt tgaccgcaag cattctatta agtgcgtgtg gcaattcagg atctgcagat
    16081 gatagtacgg ggtatgtagg aatcgctatg ccgaccaaat cagcagaaag atggatcgct
    16141 gatgggaaca acatggtctc ggaattagaa aaactgggtt ataaaacaga tcttcaatat
    16201 ggagaagata aagtagaaaa tcaagtagca caaatagaaa atatgattac aaaaggggta
    16261 gatacacttg tgattgcttc tatcgatggt tcggctttaa ctgatgtgtt agctaaagca
    16321 aaagaagcag atatcaaggt gatcgcctat gatcgtttac tgatgaattc agaaaatgtc
    16381 gattattacg cgacttttga taattttgga gtaggtgtat cgcaagcagc ttatattgaa
    16441 gagcacttag gattgaaaga aggaaaagga ccattcacga ttgaattatt tggtggctcg
    16501 cctgacgaca acaacgcact aatcaattac aacggagtga tgtctgtttt acaaccatat
    16561 atggacaacg gtcagttggt cgtgccatct ggacagacga gtttcagtca aatcgctacg
    16621 cttcgctggg atggttctac ggcgcaagca aggatggata atctgttaag cgcaaattat
    16681 actgaccaaa cattggatgc tgttttgtcg ccatatgatc caataagttt aggaatcatt
    16741 tcgtcattaa aaggcgtggg gtatgggagt gaaagtaaac ctttacctgt gattactgga
    16801 caagatgcga cagttgctgg cgttaaatcg atcattgccg gggaacaaac acaaacgatc
    16861 tttaaagaca cacgtatctt agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat
    16921 ggagaagagg ttccggtcaa tgatactgaa acttatgata atggggtgaa aactgtccct
    16981 acgtatttag caaatacagt atctgtcgat aaagataatt atcaagcaga gctgatcgac
    17041 accgattatt ataaagaatc cgatctgaaa aattaagagg aaccaaacaa ggaactaaag
    17101 agatctaagt cagagagagg ataagaatta tggcggattg tattttaaaa atggcagata
    17161 tcaccaaaaa attttctgga atccgagcgt tagattgcgt gaatctgatg attgaacgtg
    17221 gggaaatcca tgcattgtgc ggagaaaacg gagcgggaaa atcaacattg atgaatgtct
    17281 tgagcggctt gtatccatat ggctcctatg acggggaaat cttttatgat ggaaagctgt
    17341 gtcaatttaa agatttgaaa gatagtgaag aaaaaggcat cgtcatcatt catcaagaat
    17401 tagcactaag tccttatctt tcaataaaag agaatatttt tctcggaaat gaacaagcag
    17461 aaagaggcgt gattgattgg gaactgacag aaaagaaaac atatgaactg ctgaataaag
    17521 tcggtttgtc tatcgacccg aatcttctag tatcccagat tggcgtaggg caacagcaac
    17581 tagttgaaat agctaaagca ttatcaaaat ccgtccgtct gttgattttg gatgagccta
    17641 ccgctgcatt gaatgaagaa gaaagcgcga atttgctgag attgatccga gagttgaaag
    17701 agcaaggtgt aacctccatc attatttcac ataagctgaa tgaaatcgtg gatatcgcag
    17761 atcgaatcac cattttacgg gatggacaga cgattgagac actagaaaat gaaatgatca
    17821 gcgaagagca tattattcgc ggaatggtgg gcagagattt agttgatcgt tatcctgaaa
    17881 gaaaaccgga tattggagaa gtttatttta aagtagaaga ctggacggtc tatcaccctc
    17941 ttgatcacga gcgaatcata aacgaccaca tcaacttgca tttgaaaaaa ggagagattg
    18001 taggaatagc tggactgatg ggagcgggca gaactgaatt cgctatgagt ctatttggac
    18061 attcttatgg aagcaagata tccggacgta tattaaagga aggggcagag atatcagtta
    18121 agaccgttcc ttcagccatt gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt
    18181 taggactgaa tctgctgatg gatattcggg aaaacgtgac gctgagcagt ttggataaaa
    18241 taagtcaaaa aggagtatta gataaagaaa aagaagtaac tgaggcagaa aatttccgga
    18301 aaaagatgcg tataaaaaca aactccatcc atcaaattgt cagtagtctt agtggtggga
    18361 atcaacaaaa agtcgtttta tccaagtggc tgatgacaca acccgatatt ctttttttgg
    18421 atgagccaac ccgtgggata gatgttgggg caaaatatga gatctacacg attatcgaag
    18481 aaatggcagc aaatggcaaa gctgtctgtc ttatctcttc tgaacttcct gaaatattag
    18541 ggatgtgtga tcgaatctat acgatgaacg aaggacgtat tacaggagaa gtctcgcgag
    18601 aagatgcaga tcaggaagtc ttgatgaaat taatgacgaa agaagaggtg ggttaagttg
    18661 gaacaggcaa aaaatcttac ggataaaaaa gagaaaaaac taaatttgaa aagtagagca
    18721 gctgatcttt ttggtcgcta cagcatggtg atcattttag cgggactact tattgcgttt
    18781 caattgatga cggatggtat tttctggcgt ccattgaata taactaacct tgttctgcaa
    18841 aatagccata tattagtact agctgcaggg atgttattag ttgtgctctt aggtcatgtg
    18901 gatttgtcgg tagggtcagt gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc
    18961 aatcgccaaa tgaacccgtg gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc
    19021 ggcgcatggc aaggtttttg ggtagcttat gcagggatac cggcgtttat cgtgacttta
    19081 gcaggattgc tgatgtttcg tggactgaca caagttgttc taggaggcca atctctagca
    19141 ccttttccag ctgttttcca aaaaatgtct acgggttatt taccagactt aagcgaagga
    19201 agcagccttc acttcttgac attgatttta ggaatgatca tcacgatcgt gttagttgtt
    19261 ggtcaatggc gaacaagaaa tcgtcggaaa aatcatttat ttgaagtgga atctccaaca
    19321 gctttcttgg taaaggcggt atttacagca gtcgttgtac taggaataac ttatatcttt
    19381 gcctcttatc aagggtatcc gattattttg atcattcttg gtttgatcgt tgcagcatat
    19441 gggtttttga caaataaaac agtagcaggt cgacagattt atgcgacggg cggaaatcga
    19501 aaagcggctg aactatcagg gatcaaaaca aagcgaatca cgttctgggt attcgtcaac
    19561 atgggagtca tggcagcttt agctggatta gttttagctg ctcgattgaa tgcggcaaca
    19621 cctcaagcag gtacatcgat ggaactggat gcaatggcgg cagtatattt tggtggtgct
    19681 tcgacttccg gcggcattgg aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt
    19741 gtattgaaca atggcatgtc gattttaggc gtcggggtag actggcagca ggcaatcaaa
    19801 ggattgatct tgcttttagc cgttgtgcta gacttgtata ataagaaaaa acgaacaacg
    19861 taggagaacg ccggttattc agaaaaaaga ctgtgacaag acatttgtca cagtcttttt
    19921 ttcgctaaaa gcgaacattc attgtttcgt ttgtatagac taaaaataat atatgttttt
    19981 ttaccattcg tataaattct ctttttgctc ttgatttttt tgcttaattg agttaaatta
    20041 catagggcgc taaaaaaagc cttgattaaa ttaataatgt tcgtgttttg ctggatgtga
    20101 acatcaggag gataatgtaa tgaaagtatt tgattacgaa gatattcaat taattcctaa
    20161 taaatgtatc gtaaatagcc gttctgaatg tgatacaacg gtcacattag gtaaacatac
    20221 attcaaaatg ccagtagtgc ctgccaacat gcagacgatt attgatgaaa caatcgcaga
    20281 attcttagcg gaaaatggtt atttctatat catgcatcgg tttgatgaag cagctcgaat
    20341 tccgtttatc aaaaaaatga aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa
    20401 gaaagaggaa tattccttta tagagaagtt ggcagaagaa tcgctaaatc ctgattatat
    20461 cactatcgac atcgcgcatg gacatgctaa ctcagttatt gatatgattc agcatattaa
    20521 aaaatatttg cctgaaactt ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg
    20581 tgaattggaa aatgcgggcg cagatgcaac aaaagtcggg attggtccag gaaaagtatg
    20641 tattacaaaa atcaagacag gctttgggac tggaggctgg caactggcag ctcttagatg
    20701 gtgtgcgaaa gcagctcgca agccgatcat cgctgacggt gggatcagaa cacatggcga
    20761 tattgcaaaa tctgtccgtt ttggtgcgac aatggtcatg attggttcat tgtttgccgg
    20821 tcacgaggaa tctcctggcg aaacaaaagt cgaaaatgga atcgtgtata aagaatactt
    20881 tggttctgct tcagaattcc aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg
    20941 gatccaacac aaaggatctt tgaaagatac actagtggaa atgcaacaag accttcaatc
    21001 atctatttct tatgctggag gaagagatct ggaagccatt cgaaaagttg actatgtcat
    21061 cgtgaaaaat tcgattttta atggagacac aatctaaaaa ataaataaaa tattaggatg
    21121 ttgtgaaaaa gttgtcctgc attaagtaat aagaacagcc aaacaaaaat agttctttat
    21181 atgttttgtt tggctggaat tattatcgca gatacaagct tttgaacatc attattcaga
    21241 ataagggact gtgataagat ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc
    21301 ttttttctcg taaattcggt aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt
    21361 atgtgggaaa aagtagagaa aaaacaagga ccgatttatc gtcaaattat ggaacagatt
    21421 atgagagaaa tcgaaaacgg aaggatcaat cctggaagtc aacttgaatc agaacgaaaa
    21481 ctagggcaac tttttggtgt gaatcgttcc accgttgtcc atgcattgga agagcttagg
    21541 gagcttgggg tactaacgag caaacgtggc agcggatggt ttgttaatca gactgaatgg
    21601 gggaaattcg ctgttccaag aatcgattgg cggcaaatga tctctcctcg ttatgagcag
    21661 acggactggt atgagcaaaa aataagagaa gcaaagcaga tccaaaaaac atcgtttctt
    21721 gatttatacg ctagcgagat gccagatgag cttttgccga attttgagtt tccttcttat
    21781 tctcttgaag aaatcttagc ggaagaaaaa gaaatggatc ttttaggcta tactccgtta
    21841 cgtaacaaaa tcaaacggta tttggaaagt aaatttgaat tggcattttc ccccaaccag
    21901 cttcttgtga ctggtggcgg tcagcaagca atctttttga ttttacaaac gattctttca
    21961 gctggagagg ggatagctgt agagtcacct tctttttttt accgattacc tcttttcaaa
    22021 gcttcaggaa cgcgtttatt tgggataccg atggatgaag aagggatcga cttagaaaaa
    22081 ctgaaagaat cgatccataa aaataaattg aaagcagtgc ttatcaaccc gaattttcaa
    22141 aaccctacag gaaaagtgat gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga
    22201 cagtatcggc ttccaattat tgaagacgat gtatttacgg atctttcatt tctaaaccaa
    22261 gaagaagtcc ttcctatacg aacgattgat ccggataatg tgttatacgt cggttcgtta
    22321 tctagggttt taggaaaaac aacgaaaatc ggttgggtga taggacctga gacttttatc
    22381 tcgcaactag ctcaagcgca agaaatgatg gagttttcga tgaatatcct tacacaaatc
    22441 acagtggcta atgtatttaa tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag
    22501 ttgaaagaaa aaagccgact gataagcgct tggggagatt cacaatcgtt tttcaagctt
    22561 cattcaattt taggtggtta ctatgcctgg attacttggg atgggaaaaa actagacccc
    22621 gaactagcag aaaaagtagt agatatgggt cttggaattg caccaagtct actttttggg
    22681 caagatataa atggaatcag aatcaattac agtaggctgg atgaaaacca aataccgttg
    22741 ttttattcaa agatgaaaat tctagaaaag tggttaacag aataatttca caaagtaaat
    22801 agattaaaga atataacagc ttggatttaa atttcaagct gtttcttttt tttcggaaaa
    22861 agaatacgat ataaagaaac taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt
    22921 aaataaaata aatgtttgca acaaaactgt ataacaccaa cgtttttaaa tagaaacaga
    22981 tggttgtaag tttgattaga aaatagaaaa aatgcagatg aaatataaaa cgctttcctt
    23041 attgtgattt tattcactaa tgctgttaat atgcggattg gcataatttc ctttcatcgt
    23101 gaaaatttgt gcttttagct tgtatttctc ataaatccat gataatttat taatgaagac
    23161 ggttaaatag tgatacagtt ttattcactg tttcaatcgc agaatgaaga gattttctta
    23221 tagatgagag aggaatgtgt gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc
    23281 actgctagat gaagtcaatg cagatttccc agtatatcaa gcacttgatc aagatggaaa
    23341 cgtagttaac aaagatttag tacctgattt atccgatgat gaactcgttg aattaatgac
    23401 tcatatggta tggtctcgtg tattagacca acgttcaaca gcgctgaacc gccaaggacg
    23461 cctaggcttt tttgcaccaa ctgctggaca agaggcaagt cagctggcta gtgcttacgc
    23521 atttgataaa gaagatgttc ttcttccagg ttatcgggat gtacctcaac tagtaaaaca
    23581 tggtttacct ttatctcaag cttttttatg gtctcgagga catgctgcag gtaattctta
    23641 tccggaagaa ttgaaagctc taccaccaca aattatcatc ggtgcacaat atgtacaagc
    23701 agccggtgtt gcattaggtt tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac
    23761 tggtgatggc ggttcatcac aaggtgattt ttacgaagca atcaatttcg ctggcgctta
    23821 ccatgcaaat gccgtatttt atatccaaaa caacgggtat gcgatctcta cacctcgtga
    23881 aaaacaaact gcagcaaaaa cacttgcaca aaaagctgtc gcagctggga ttcctggtat
    23941 tcaagtcgat ggaatggatc cgcttgcagt atatactgta tctaaaatgg cacgggaatg
    24001 ggcgatcagt ggaaatggac cagtcctaat cgaaacactg acttatcgtt atggaccaca
    24061 tacactatct ggtgacgatc caacccgcta ccgtacaaaa gacacagatg acgattggca
    24121 gaaaaaagat ccattgatcc gcttccgtaa atatttgacg gaaaaaggac tttggtctga
    24181 agaaaaagaa gaacaagtca tcgaagcaac taaagaagaa atcaaagcag ctattgctga
    24241 agccgacaaa gtaccaaaac aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc
    24301 tccacaatca atcaaagaac aaattgcaat ttatgaagcg aaggagtcga aataatcatg
    24361 gcacaaaaaa caatgatcca agcaatcaca gatgccttag cacttgaact agaaaatgac
    24421 gagaatgtcg ttgtttttgg tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact
    24481 gaaggattgc aggaaaagtt cggagaagac cgagtattcg atactccttt agctgaatct
    24541 ggaatcgcag gattgagttt cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc
    24601 caattctttg gttttatttt cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca
    24661 agatatcgaa tgagcggcac tagaaatcta cccatcacaa ttcgttcgcc atttggtggc
    24721 ggtgttcata caccagaatt gcattcggat aatttagaag gtttgatcgc tcagtctcct
    24781 ggtattcgag tagtgattcc ttccaaccct tatgatgcaa aaggactatt gatttcttct
    24841 attagaagta acgatccggt tgttttccta gaacacatga aactatatcg ctcattccga
    24901 gaagaagtgc cggatgaagc ttacgaagtt cctttggata aagcagcagt tactcgtgag
    24961 ggaacagatg tatctattat cacatacgga gcaatggtgc gtgaagcaat caaagcagca
    25021 gacaatcttg caaaagaaaa tatatctgtt gaaatcattg atcttcgtac agttgcgcca
    25081 ttagatgtag aaacgatcat tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa
    25141 gaagcacaac gacaagcggg cgttgccgcg caagtagttt ctgagatctc agaacgcgct
    25201 atcctttcat tagaagcccc aatcggacga gtttctgcac ctgatacagt cttcccattt
    25261 ggacaagcag aaaatgtctg gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa
    25321 atcgcggaat tttaagaaaa gaagggacga aacaaagtca tggcttatca atttaaatt
//
LOCUS       DACEMS010000034        25111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEMS010000034 DACEMS010000000
VERSION     DACEMS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.034"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            365..1276
                     /locus_tag="IW735_001209"
     CDS             365..1276
                     /locus_tag="IW735_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1044222.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1493..1930
                     /locus_tag="IW735_001210"
     CDS             1493..1930
                     /locus_tag="IW735_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044223.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            2025..2909
                     /locus_tag="IW735_001211"
     CDS             2025..2909
                     /locus_tag="IW735_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1044224.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3133..6300
                     /locus_tag="IW735_001212"
     CDS             3133..6300
                     /locus_tag="IW735_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044225.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6320..6865
                     /locus_tag="IW735_001213"
     CDS             6320..6865
                     /locus_tag="IW735_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1044226.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            6893..7348
                     /locus_tag="IW735_001214"
     CDS             6893..7348
                     /locus_tag="IW735_001214"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1044227.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            7345..8103
                     /locus_tag="IW735_001215"
     CDS             7345..8103
                     /locus_tag="IW735_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1044228.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8189..8929)
                     /locus_tag="IW735_001216"
     CDS             complement(8189..8929)
                     /locus_tag="IW735_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1044229.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            8990..10243
                     /locus_tag="IW735_001217"
     CDS             8990..10243
                     /locus_tag="IW735_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1044230.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10565..10792
                     /locus_tag="IW735_001218"
     CDS             10565..10792
                     /locus_tag="IW735_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1044231.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10915..11241
                     /locus_tag="IW735_001219"
     CDS             10915..11241
                     /locus_tag="IW735_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1044232.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11309..12022
                     /locus_tag="IW735_001220"
     CDS             11309..12022
                     /locus_tag="IW735_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1044233.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12332..13183
                     /locus_tag="IW735_001221"
     CDS             12332..13183
                     /locus_tag="IW735_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1044234.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13304..15169
                     /locus_tag="IW735_001222"
     CDS             13304..15169
                     /locus_tag="IW735_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1044235.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15217..16626
                     /locus_tag="IW735_001223"
     CDS             15217..16626
                     /locus_tag="IW735_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1044236.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            16734..17435
                     /locus_tag="IW735_001224"
     CDS             16734..17435
                     /locus_tag="IW735_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1044237.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            17594..18073
                     /locus_tag="IW735_001225"
                     /pseudo
     CDS             17594..18073
                     /locus_tag="IW735_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            18921..19496
                     /locus_tag="IW735_001226"
     CDS             18921..19496
                     /locus_tag="IW735_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1044238.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            19496..19936
                     /locus_tag="IW735_001227"
     CDS             19496..19936
                     /locus_tag="IW735_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1044239.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            19952..20653
                     /locus_tag="IW735_001228"
     CDS             19952..20653
                     /locus_tag="IW735_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1044240.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(21141..21566)
                     /locus_tag="IW735_001229"
     CDS             complement(21141..21566)
                     /locus_tag="IW735_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044241.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            21940..22911
                     /locus_tag="IW735_001230"
     CDS             21940..22911
                     /locus_tag="IW735_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1044242.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            22943..24340
                     /locus_tag="IW735_001231"
     CDS             22943..24340
                     /locus_tag="IW735_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1044243.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            24499..25107
                     /locus_tag="IW735_001232"
     CDS             24499..25107
                     /locus_tag="IW735_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044244.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         8573 a         4132 c         5295 g         7111 t
ORIGIN      
        1 tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga acataaaagt
       61 gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac atttgttcta
      121 agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta acaaaagaag
      181 tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa ctttctggat
      241 tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg ttcttgatga
      301 ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt aggagaattg
      361 ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct tattgtcagg
      421 ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa tcagcgaaca
      481 agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc aacagttgat
      541 cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag aaatcgattc
      601 tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat tgaaatctgc
      661 aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct ttcaagaagg
      721 tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg aatctttcca
      781 tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa aagatgtgaa
      841 gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt caactgatac
      901 tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa cggttccggc
      961 agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg tgatcacttc
     1021 taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga aaaaccaaaa
     1081 caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa cagacacaaa
     1141 attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag acgctaatgt
     1201 aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag attctagtac
     1261 agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc aataaaactg
     1321 ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta cttgatttag
     1381 ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat atttttctgt
     1441 ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat aaatggagtg
     1501 gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg tttttctcac
     1561 aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc atcaattttc
     1621 cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta ttcaaggccg
     1681 tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc ctctgatcgc
     1741 tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc tatttttgtg
     1801 tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat cattttcttt
     1861 tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt tttatcttgt
     1921 caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt ttttctttat
     1981 gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa aaagttttat
     2041 tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa ttagcgatag
     2101 caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa gcaggggatg
     2161 caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt gtcatgggcg
     2221 gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat cggccaaatt
     2281 tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg ataccgctag
     2341 aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg gatattggaa
     2401 aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct gaatcaatca
     2461 atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc atgtcaggaa
     2521 tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat ggaaagaagg
     2581 aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc ggttttgaaa
     2641 cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat ttaaaagatt
     2701 cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta gatgaatcta
     2761 cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct gatgaggaag
     2821 aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca atcaaagtat
     2881 tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga gttggcggga
     2941 gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc cttttttttg
     3001 tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca tattttaaat
     3061 atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa acgtcagaga
     3121 ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt gtttgtcttc
     3181 cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga atcaacgcaa
     3241 tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac ttctgaaact
     3301 acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa gacaagtgag
     3361 acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc aagagctgtt
     3421 tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct tctcgcagac
     3481 gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga attagagatc
     3541 aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac acaccatatt
     3601 tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac agctgcacat
     3661 ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta tatgaccgat
     3721 ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac acttgatttg
     3781 tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac attagatggt
     3841 gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc tccgggtaaa
     3901 gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact ttatggaccg
     3961 tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag tggaaaaaca
     4021 gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga tcgggcagat
     4081 ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat gtttgaagtc
     4141 agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa tgacaacaat
     4201 cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa tggtgggaaa
     4261 atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc tagtcaaatc
     4321 catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga tcaacccggg
     4381 aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg agccggatca
     4441 gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga gaatggtacg
     4501 caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg tgatggcaaa
     4561 tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga cgcttctggt
     4621 tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc aaatatcgat
     4681 atacgcgcag attccggagc aactgtagat acaactggag atattgattt aaattttacg
     4741 gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa gaccggcatc
     4801 atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga tttccaaaac
     4861 aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg aaatggagat
     4921 tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg cgcaacattc
     4981 gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc gatcaatggg
     5041 accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga atttgctcag
     5101 acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct cccgatgatt
     5161 gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt cgttgttccc
     5221 gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt gactttacgt
     5281 attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac gcaaggacaa
     5341 acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca aggcggcttg
     5401 ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt gcaagttgtt
     5461 gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc caaaacgaca
     5521 gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa tgcagcttct
     5581 cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa aaatcaagct
     5641 gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat cccagaaaat
     5701 caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg agaagctact
     5761 gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca caatccaagt
     5821 gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa gatcgtagta
     5881 gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat caaagcaact
     5941 ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg gatttatgat
     6001 cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc tttcacaaat
     6061 gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga gtatcaatta
     6121 gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt gcaagcagat
     6181 tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa agaaaatcag
     6241 atagctggta attactcagc agagataaca tggacattag cagatgcgcc gacagaataa
     6301 agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa acaactgctc
     6361 tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc atgcctccga
     6421 attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga acaaaacata
     6481 tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg tctttaaccg
     6541 aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga attcaaacgg
     6601 tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt atgatatgaa
     6661 aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg tcactgagac
     6721 actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag ggatcacgtt
     6781 tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca atcagttttc
     6841 tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag atttgaaatt
     6901 aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga atcttcaaaa
     6961 tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca aaaaagaaac
     7021 aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca atacaaattt
     7081 tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc aagcggttgt
     7141 gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa taactgatga
     7201 tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag aaacatttga
     7261 ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg gattatttat
     7321 tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac cgttctagct
     7381 ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc agccactaca
     7441 gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt tgttccagtt
     7501 cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc aaatcaggga
     7561 acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac aatcaagtta
     7621 ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac tggagcacaa
     7681 acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc cggttggaaa
     7741 ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa actaactggt
     7801 tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag cagtggaatg
     7861 ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga tcaggcagta
     7921 gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc attaggagag
     7981 aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa ttatgctgta
     8041 gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga agaggaagcg
     8101 taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc cctgaaatct
     8161 gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg aagtcatgga
     8221 aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca ccgttgaaaa
     8281 attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg atacagtaag
     8341 attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt tttaaagaag
     8401 tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc aaacctttga
     8461 tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct ttttgatcaa
     8521 taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt tctagcactt
     8581 gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga aggattttcc
     8641 gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata ttactatgtg
     8701 catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc ttcaagtcga
     8761 tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac tgattgattt
     8821 gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat tctctgatga
     8881 tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata tgcaggctcc
     8941 tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga tggtcgcaac
     9001 agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt tattcttttc
     9061 gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg tgacactttt
     9121 acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga tgtttctttt
     9181 ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt tttttgctat
     9241 cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa agagacatct
     9301 tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat tagtaaaaag
     9361 tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg aacactattt
     9421 tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg gaagtgttca
     9481 cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca gttttttttc
     9541 tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac atcaggaatc
     9601 gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat tcaaacatga
     9661 ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa aagaaacgtt
     9721 ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac cgcaaataca
     9781 acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat ttctagaaaa
     9841 agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga aaacaatccc
     9901 gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg cattcgatca
     9961 tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag agggatcacg
    10021 ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga tggtcaaaag
    10081 aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa aaatgttgga
    10141 tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact atttttttgt
    10201 agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg tcaaaggata
    10261 agtttgagga aagtctacac ggagatagag acaacaacac agcatattca aaagaaacag
    10321 aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa caaagtagtt
    10381 gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa gtagataaga
    10441 agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca ccagataaag
    10501 ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta cgatcacaaa
    10561 aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc atcgaaaagc
    10621 ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa aatcattaag
    10681 agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac aaaaagcctc
    10741 aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt gattttctat
    10801 gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc tattttttat
    10861 gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa gcaaatgaat
    10921 agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt tcataacgaa
    10981 aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact ttttcaatgg
    11041 aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga actggcagat
    11101 gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga tgagatcatt
    11161 gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc aggtgtacga
    11221 aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc aaaagaaaaa
    11281 cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa aaagatggct
    11341 agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt tcaatgaaca
    11401 gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga atgtaacaaa
    11461 agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg cggtagaacc
    11521 aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac cagtgattgc
    11581 tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct tggataatac
    11641 gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa aaggaaatta
    11701 tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc tagaaaattt
    11761 agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat acaaaacatt
    11821 ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg agggaaaaga
    11881 aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag ttgtacaagg
    11941 agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat ctgctttcaa
    12001 tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt aaaaacagcc
    12061 aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa cgcaaagagg
    12121 tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt tattttttat
    12181 atgaaaaggc ttgcataaat aaaaattcga tggtatgatg attttgaata agaaatggat
    12241 tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg aattctaggt
    12301 ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt tgaataacaa
    12361 tgtcgtcatt tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa aaggactagc
    12421 ttttcaaaaa aagattgggg acgagatttc gccagaagcg gtccaaaaag aatttgtttt
    12481 aagagattct tttgctaaaa accaattcca gcagctgatg gctgatattc cggcagagga
    12541 gatcgaatgg gtcaaacaag tagtcgaact ggctgaagat aaactaaaag ttgaattttc
    12601 gcctaatatt tatctgacat tgaccgatca tcttcattat gcgatcacac gagcaaaaga
    12661 aaatatccaa ctgcctaatc cattattatt tgaaacaaaa aaattttatc cgaaagaata
    12721 tcaagcagcg aaagaggctc ttttgttcgt gaaacaaaag acgggtgtgg aactttctga
    12781 ggatgaagcc ggttttattg cctttcattt tgtgaatagc caacaaggaa acgaagacat
    12841 gcaagtgacg atgaccgcta ccacaatggt aagagatata ctgagtatca tcagtaaatt
    12901 cttcggcatc gtgttcgatg aagaatcttt gaattatcag cgtatcatta ctcatctgca
    12961 gttttttgcc cagcgttatc tcaaagggga aggatctgac gagcaagatg aatttctcta
    13021 cgcattagtt caaggaaagt atccaaaagc gtttcattgt gtcgaacgga tcaatgagta
    13081 tcttcgacaa acgcaaaaac aggaaatggg gatcgcagag cagatttatt taacgattca
    13141 tattcaaaga gtagtaagtg agaaaaaaag catccaatca taaggattgt tactgtatgg
    13201 caggcaaaac ctggattgaa aaaataggtt cacgaatgtt tgtgactgtt tttttcaatc
    13261 tgggttttta ttttttaaat aaaaaagaaa gaaggaaaaa acaatggata atcaagcagt
    13321 tggtcgtcgt gtatgggaag cggtaggagg acaaaagaat gtcaaaagtt tagtacattg
    13381 tgctacgcgg ttgcgattca gattaaaaga tgaatcttta gcagatacgc aaaagttaaa
    13441 agaagacccg gacgtgatcc aagtcgtaca aagtggcggt cagtatcaag tagtgattgg
    13501 cagtaatgta gcagatgttt atcaatcgat cgtggatgag gaaggtcttg cagtttcgga
    13561 ggatggaaaa gaagagtcca aaaatatttt gaatcgtctg atcgatatta tctctagtat
    13621 atttacacct tttctagggg cgatggcagc cgccggggta ttgaaaggat tcttatcatt
    13681 ggcaacggtg atgggctggc tgacagctga tagcggtact tatcaaatat tgtttgctga
    13741 agcagatggt gtctttacct tcttacctgt catgcttgct tttacagcag ctaaaaagtt
    13801 caaagccaac cagtttttag cagtagcaat tgctatggca cttgtgtatc cagctatcac
    13861 agcagttgct ggtgcgggag aagctatcag tttctttggg atcccagtga ttctttcacc
    13921 atctggttat acttcttctg tcataccaat cattctagcc gtatgggttc aaagtaaatt
    13981 agagccattc gtaaaaaaag tgattccgca atttctgcaa atgattttag ttccgcttgt
    14041 cgttttagta gtgatggttc cattaacatt tcttgcttta ggaccaatcg ggacggttgc
    14101 cggtaatgct ttaggcggtc tcttcaactc gatttatgga tttagtccaa tcgtagcagg
    14161 tctaatcatg ggaagtttat ggcaagtatt cgtgatgttc ggcatgcatc ggggattcgt
    14221 accgatcatg tttttgaata tcgaacaata tgggtttgat gtattgatgc caatgcttct
    14281 tccagccatc ttggcacaag gtggggctgc tttagctgta gcattgcgaa caaaagatac
    14341 gaaacttcga gcattaggga tttcttctac agtgacttcg ttatttggaa tcactgaacc
    14401 aactgtgtat ggtgtgacat tacctttgaa gaaaccattc attgccgctt gtatttctgg
    14461 aggtattggt ggagctatta tcggattttc cggagtaaaa gctttctcca gcagtttggt
    14521 cagcttattg acgattccga ctttcattaa tacggtggat ggtgtagagt caaatgtgac
    14581 agtagcagta atagctacag gaattgcttt tgttcttgca ttcgtaggta cattgatttt
    14641 aggatttgac gaacaaacac aagacaatca attagaaaac aaacatgcga atgcaggaga
    14701 accgattact tcagcacggc ataccttaaa aagtccttta acgggtaaag tactgccatt
    14761 gagtgaagta ccagaccaag ttttttcttc tggcgtgatg ggaaaaggta tcgctattga
    14821 tccagaagta ggagaactag ttgcacctgc agacggagag atcacgacta tttttccaac
    14881 tggacatgct gtagggatca caacgactga cggagcggag attttgattc atatcgggat
    14941 ggatacagta gaattaaatg ggaatggctt tgagatctta gtcaaacaag gggatctcgt
    15001 caaagcagga gatttattga ttcgttttga catcgaagca attagagcag ctggctatag
    15061 cgtaatcact ccagttgtca ttacgaatac tgatgcattt gccgatatac ttgaattaga
    15121 ccaaaaagag attattgcaa acgaagatgt tctggcaatt gtaaaataag gaggaaatca
    15181 ggatgcaatc acgtttttca gaaaaatttt tatggggtgg agcaactgct gccaaatcaa
    15241 ttagaagggg cttatgatcg agacggaaaa ggactatccg ttgcagatgc tatgcctgga
    15301 ggtaagcagc gctttgcgat tatcggcagt gaagaatttg actggacgat tgatcaagaa
    15361 aaatatattt acccgaatca tcgaggaatc gatcattacg atcgtttcaa agaagatctt
    15421 gccttgtttg cggaaatggg attcaaatgt tatcgttttt cgattgcctg gacacgaatt
    15481 tttccaaatg gagacgaagg tactcctaac gaagcgggat tagaatttta tgatcagctg
    15541 attgatgagt gcttaaaata tgatattgaa cctgtgatca cgatttctca ttacgaaatg
    15601 cctcttcatt tggcaaaaga atacggtggc tggaaaaatc gaaaactgat cgacttctat
    15661 gaacgatttg ctcagacagt attagagaga tacagcagta aagtcaaata ttggatgact
    15721 ttcaatgaaa tcaattcggc ctttcacttt ccagctttaa gtcaaggatt agtaaaaagc
    15781 aatggagcag gagagtacca aaatattttc caagcatggc ataatcaatt cgttgcaagt
    15841 agcaaagccg taaaaatcgg ccacgaattg cgttcggata tccaaatcgg ctgcatgatt
    15901 atctatgcaa caacttacag catcgatgcc aatccagtca atcaggcagc aacaatgatc
    15961 caaaaccaag aattcaattt tttctgcacg gatgtccaag tacgtggaga ataccctgca
    16021 tacacagcgc gtacccataa aaaatatgga gtcgatccgg aaaaattaga gcaaacggaa
    16081 aaagacttcc agttgctaaa agaatatcca gtggactaca tcggatttag ttattatatg
    16141 tctacggcaa tcaatgagac tgatccacaa gcagcaactt cagaagggaa cttgctggga
    16201 ggagtaaaaa atccgtttct tgaggcaagt gaatggggct ggcagatcga tccagaaggg
    16261 ctgagaatcg cattgaatga attatacaat cgttaccaga aaccattatt cattgttgaa
    16321 aacggtcttg gagccattga tcatatggaa gaggatggca cgattattga tgattatcgg
    16381 atcgattatt tacgtcgtca tatcgaagcg atggctaatg cagtagcaga cggtgtagat
    16441 ttgatgggct atacgccttg gggatgtatc gatcttgtca gtgcatcaac aggagaaatg
    16501 agtaaacgtt acggctttat ctatgtggat ctagacgatg aaggaaatgg gacactgaat
    16561 cgatcaaaga aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa tggcgtaaac
    16621 ttgtaattaa agcagctgaa tgaaaaagaa cacacttctc acatgtgatt tgtgttcttt
    16681 ttttattttt tatgtgctat tcttttagta atagtaaagc aaagggtgac gttatgaaaa
    16741 ggacacgtgt actatacttg gaagtcgcag ataaaattaa agaagatatt ttctcaggaa
    16801 aatatccagt cggttcgatg ttgccgacag aaagtgaatt agaagaactg tttcaagtca
    16861 gcaaaatcac tgtccgaaag gcaatcgaac tgctggcatc agatgaatat gtcgcaaaga
    16921 aaagtggtcg tgggacgacg gttctaagca atcgtcctta caatcgatta tccaaagcgg
    16981 caagctttac gcaaatcttg gagaactccc gatatgaagt ccggaaagaa acgctggatt
    17041 tgcaattggt ccatataaag aaggaagatc ctttgttttc ctttttcggt gagcaagctg
    17101 tttgttttaa gcgcttatat tatttgaaca atcagcctta tatttatttt gttcattatc
    17161 ttccacaaga aatgatgcaa ttcagcagag aagaatttgg caaagaatct ttgtatcgtt
    17221 tgctgacgaa aaaaggatat actattgact cattttctga tgatttctcg gcagtatttt
    17281 taacagaaga agaacaagga attctgaaaa cagacgcaca gatcggcatc aaacggatac
    17341 ggaaatcgtc tacgcaaatt ggggaaatga ttgaatattc ggaggcggtc taccatacgg
    17401 agctgcatcc ttaccatatt gattatgaaa cataaataaa aaaagaggtt gtgaaaaagc
    17461 tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg
    17521 gttaggctta ttaccgcaga tacacggctg tttctggatt accatttatt ctggataagg
    17581 gggtgtggca cgattttcag aaggaatcta tgaaccaatc actgtattga ttttagcttc
    17641 gatggtaacc ccattcggct tgacgatcgc ttatttctta gggaaaatca ttcgtaaaaa
    17701 tatactcaat cgtcaagaaa tcgatacatt gaagaccgct tttcctatgg ggatctgtca
    17761 gatcacagag ggatgtttcc ctatcgtatt gaacgacttg gcacgcaatg tcatagcaac
    17821 gggagtcggt gggacagtag gtggcggatt aagcatgttt tggggcgcag atagccgtat
    17881 tccggcatcg ggaatgtttg cagtcgctac gatgaccaga ccatgggcat tcattggtgc
    17941 attgttagct ggttcttttg tcacaggaat aaccatttta ttattgaaaa aaccagtaga
    18001 tccgaatgca gaaatcatcc aagaagaaaa agaggaagaa gacatatctt gggatgattt
    18061 gacaattagt taggagaggc agaatggatt acaagaaaaa agtacaatag atgtaccttg
    18121 agacaggaat ggtatgaaag aatcagagtt agaaagaatc gaatatacag attttggttt
    18181 aggagcgatc gaaactgtcc agagcatcgg caccctcctt gaaatgacga tgaaggaaaa
    18241 atgagaacct ttcgttgtcg aaagggtctg gtatacctta tgtcgaagga aaggaaacaa
    18301 aaaaactttc ctgtactatt ccaaaaagga aagaaagtta ttatacagta aaaaaagaaa
    18361 ttattcttca tgctagagat cagtacacgt tcgagcccaa tatcaaacac tagttccagg
    18421 caggcgcaac aggtgcggtt atcactgaat tttcttccag cagtgacgat tcaagtgata
    18481 tttttacaaa tccagctatt tatcgttaac aaaaagattt ttaggagaag ctgatgaaca
    18541 aaaatgatca aattttgatc gtttagttcg tcggcttttt tatttagaga aacatcattt
    18601 atcagtgaaa cataacacga gaacaaaatg attttttcgt taataaaaga aacaagtatt
    18661 tgttgtttaa aataatacac gttattggca tataggacga aatcccagta tcttctataa
    18721 gtaagtcatt tcatatcgat gaaaacacgt tatttatttt tgttttatct ataaaatggc
    18781 tgaaaaatga tttttttcta aatactatat atagtggcgt ctatcctata taccactaaa
    18841 aaactacaaa atctagtctt ttattttaaa aaaacacgtg tagaatgaag atagactttt
    18901 gagttaggga gtgttttttt atgaaactta ctgttttttg cggttcccgt tttggtaata
    18961 aagaaagtta caaatttatt gcgcaaacgt taggaaaata catggcacaa gaagaaatcg
    19021 aactggttta cggtggttca tactccggga ttatgggagt gattagtaaa acagtactgg
    19081 aaaataacgg aaaagtcaca ggtatctatc cgaatgggct ttttgaaact gaattgcctg
    19141 gaaaagatgt tactacattt attcctacag aaacgatcga tgaaagaaag gtattattat
    19201 ttgaaaaggg agatgctgtc ctcgttttcc ctggaggtct aggtacattg gaggaattct
    19261 cacaacttct ttcttggata gcaatcggtc tgacgcccga caagccaatc ggaatattga
    19321 atattggcgg ttattacagc ggcttgcaac aattattaga aacttttgca aaagaggatt
    19381 ttatggataa aaaatggttg gatcaagtaa tcttttccaa caatccattg gaactggtca
    19441 atgaattacg agcagtatct attaaaaatc agttattatt gaaggaggca aaataatgag
    19501 tcaaatatat ttagcaggac catttttttc agaggagcaa atcgatcgag taagtcgtat
    19561 cgaaaaagcg ttggaggaaa acaaaacagt cacaagtttc tactctccac gtcatcacca
    19621 agaaagcaat tatgaactat tcagcgctgg atgggcgcag gaagtatacg aaaaagatat
    19681 ggaagaactg acaaatgcag aatttgttgt tgctatcctt gactttgagc atcaaacaat
    19741 cgatccagga acggcttacg aattaggtgt ggcaactatg ttgaaaaaac cgatgatcat
    19801 tgtacaagaa gagacggtac ctacgaactt gatgattaca caaagtctcc atacgtattt
    19861 aaaatcagac caagctgtac gtgaatatga ttttgaaaca ttgcctgttg aaacttacgt
    19921 aggcgagtac ttatagaaga ggaagggttg tatgtcagtg attttgattt caggaacgat
    19981 tggtgcaggt aagagcagtt tgacggatat gcttgcaaaa gagattgatt ctaaaccatt
    20041 ttatgaaaat gtcgaagata acgaagtatt gccgctcttt tattcaaatc ctgagcagta
    20101 cgctttttta ttgcaaatct tctttttgaa caagcgattt ttagcaatga aaaatgcttt
    20161 agtgaacgat gacaatgtgc tagaccgatc catctatgaa gatagcctgt tgttccatct
    20221 aaacgctgat ttaggtcggg taacggatat cgaagtacag cagtacgata gcttattaga
    20281 tacgatgcta aacgagttag atgatgttgc tccaaaaaaa cggcctgatt tgatggtcca
    20341 tatcaaagtt tctttggaca cgatgctaga acgtatcaaa aaacgtgggc gtgactatga
    20401 acagctagaa agcgatgaga cgttatatac ctattatgaa acgttgaaca cccgctataa
    20461 tcaatggtat gaagattttg atgtatgtcc taaaatacaa gtggatgggg ataaattcga
    20521 ttttgtagaa aatccagaag atgcaaaaaa ggtgatccgt cagatccaag aaaaattagc
    20581 tgaattggaa ggaaagcctg ctggaaaata ttttacggat aaccagcatg caaaaggtca
    20641 agaattaggc taaaaataaa aaaccaaacg acgggaaaga aaaattacta cgtgatgtag
    20701 aataagcctt tctatcgttt ggttatttta tattcaaata tttgaaataa ataaatcttt
    20761 actctttgat ttgtaaactg aaatgatcca ctgtttgttt tactgatgaa gcttgatcaa
    20821 aggaagaagc tgagtttgaa gttcataaaa atcagccata gtgagttttg cttggatttg
    20881 tttatcttct tctacttttt acataatttt gaacaggata agattcaaat gttttaaatg
    20941 aatctttgac tagtttgtga cagttgtcgc atatgctcaa gcttccttgg tcattaaatt
    21001 ctgtatggat atttttgttg ttattacatg tacaataatc aggactatcg atcatccatt
    21061 tcgtctcctt tacaatcatc ttgcttcttt actatagcag aaaagaaggg gagaaacaaa
    21121 agaagatcca acagacggca ctatagactg tagatgttgc catcctcatc cttcaaacgg
    21181 taattaccga tttttgtaaa tatgactgtt gtttcttttt tattttcttt tcctgaaata
    21241 atatattcgt catctttggt agtgatcttg atatctgtca gcactgtttt ttcttcagat
    21301 gattcagaag aatcagttcc ttgtgtctga tacgtgcgtg tccattccat ttttttatct
    21361 gtaaatgtca actgtcccgc ggataaatcc atatctttac gtacttttag cgtatataca
    21421 ctgttggtgg tatctgcttt ttccgctttt aagacagaag aatcggttgt ttcgtccgag
    21481 gattggtcag agccgccaaa caaatcacag ccagtcaatg tcaaaagtcc cgcagacaaa
    21541 ataaatagta cgattaattt cttcaaaata cttcccccat ttgataggat aaagaaatta
    21601 taccattgga atgtaaaact tcaaacggat ggaagagaaa aacgcacaaa acacccacaa
    21661 atagagtaaa ttttagaata aaattttaaa aaggcaaaaa atccgccact ttcagaaagc
    21721 gttttaaaaa agtgttgcaa tcttttaaaa aaatgctata ttaaaaatga accggttcaa
    21781 atacttgttt aagtataatt aatagtgaga ggaagttgct ctactgaaga ctttagttat
    21841 atggatattg atgacaagca acactatgca aagaatgcgg tcatcataat cgaacaacaa
    21901 gaaagagaaa tcaaatgtaa ctggcaggag tgagaatgta tggcatcgat ccgtgatgtg
    21961 gctaagatgg ctggtgtttc agtgggaaca gtttcccgtt acttgaatgg tcagcaactg
    22021 aaggaaaaga acatgaaaaa aatagcagaa gcgatttcag cgttggacta taaagaaaac
    22081 atcattgcta aagggttgaa gaataatcga agcttctcta ttggactttt gataaacggg
    22141 atttccagcc gttttgggtc ggaagttgtc tcagggatcg aaagagtagc tgaagaaaat
    22201 gggtatagct tattattaag cgggtttgcc gatcagccag aaaaaattga acaaaaaatt
    22261 gaatacctga tgaagcatgt gatcgatggg ctgattgtct tcttatccga agaagagtgg
    22321 tctggatttg aaatgctcac aaaaatgagt attccagtta tcgcattgaa ttgtccagaa
    22381 ggaccagcag gtattgatac aatattagtg aatgatcgag aaagtgtagc aaaagtgatc
    22441 gctcatcatg gtgaacaagg acataaaaaa atcggattta ttgctgccac tcaaacagac
    22501 tacgtagcaa gagaacgttt agcaggagcc aaagaagcgg tccaaaatga tccatcgatc
    22561 caattagagg tctttacagg agactattct agaaaaagcg gctattacgg ggcaaaagca
    22621 ttattagaaa aaggggcaac tgctattttt gtcagcaatt acaatatgtc gattggtgcc
    22681 atcgaatgtt tcaatcaaga aggtgtcaga atcgggaagg atattgcgtt cagtcactat
    22741 gattatttag ataaaaacga tctgagtatc cttccgaaaa tcacgatcac gccaccaaca
    22801 gataaaatcg gggaacgagc agcagaacag ctgttaaaac gaatcaaaaa agaagaggaa
    22861 gaaaatgaaa aagtgattgt gatggaaaat atcatacatg gaatcaacta aaacgaacta
    22921 aaaaagaaaa agaggtgctt tgatgaaaaa atatcaattt ccagcaaatt tttggtgggg
    22981 ttctgcagca agtggcccac aaacagaagg aaggatttca ggcgatggaa aaggagagaa
    23041 catttttgat tattggtaca aaaaagaacc gcaaaagttc tttgatcaag taggacctga
    23101 aaaaacctcg caagtttata cgaagtatca agaggatgta cagttgatga aacagactgg
    23161 gcataattca tttcgaacat ctatccaatg gagccgtttg atacctgatg aatctggaac
    23221 aatcaacgaa gaagccgttc gtttctatca tcgttatttt gatgagttga ttgaaaacgg
    23281 tatcgaaccc tttgtgaatc tgtatcattt cgatatgccg atgtatctgc aagaaaaagg
    23341 cggatggttg aaccgagaaa cggttgaagc ctatgaaaaa tatgccaaaa gttgttttga
    23401 attgtttggt tcgaaagtaa aaaaatggtt tactcataat gaaccaatcg ttccagtcga
    23461 agcaggttat ttatatcgat ggcattatcc agaagagtca gatatgaaaa aagcgatcca
    23521 agttggctat catgaagcgt tggcttcagc gttagccatc aaatcttacc atgaaatgga
    23581 gcaaggtggt gttattggta tcatcttgaa tctgacacca agctatccaa gagacgaatc
    23641 aaaccctgaa gatgttaaag cagcgcaaat agcagatgcg ttcttcaatc gttcattcct
    23701 tgatccagct gtaaaaggcg agtttccaag tgaattgatt gagatagtaa aagaacttga
    23761 tctcatgcct gtgatccatg cagaggattt gacggttatc aaagaaaata cagtagatct
    23821 tttaggtatc aattattacc agccacgccg ggtaaaagca aaagaaacac caatcgatac
    23881 taaaaacggt ccaatgcctg aggattattt cgacaattat gaaatgcctg gtcgtaaaat
    23941 gaatccttat cgcggatggg agatctatga acaaggaatc tatgatattt tgatcaatgt
    24001 acgagatcat tatggaaata tcgactgctt tatttctgaa aacggtatgg gtgtagaaaa
    24061 cgaagcacga tttatcaaag aagatggcat gatccacgat gattatcgga tcgaatttgt
    24121 ccaatctcat ctgaaatatg tccatcaagc cattcaagaa ggtgccaatt gtttaggcta
    24181 tcatatgtgg acatgtatgg ataactggtc gtggaccaat gcatataaga atcgatatgg
    24241 ctttatttca gtagacttag cacaagatgg aaaaagaaca atcaaaaaat cgggtcattg
    24301 gttcaaagac gtatcagata aaaacggatt tgaggcttaa gaaagaatag ttgagagcaa
    24361 atgcgtgaaa ttctccattc ttaccatttt gtgaaaaaga agttcaatta tttacgatct
    24421 ttttcaactt ttttgtcttt ctatttcatt ttttgtatat aatagaatag tcaaaattaa
    24481 ataggagtgg aggaaagcat gaggaaaaaa agaccgtttc ttctcttttg gttattcggt
    24541 tttttcttac tgcttatttt atccgcagca ttttcaggaa aaatgacagc agatcttgct
    24601 cagaaagaaa cagaaaaaat cgtagcaagc aaagaatcag tagataattt caaaaaagaa
    24661 ttctcaactt attggtgggc ggagtataaa ataactgcca attacgaaaa aggcgaattg
    24721 gacatttatt tgccaaaagg aaaagaagaa aatcaattta ttaatatcga tgacgtactt
    24781 agtgcgattt ctctcagaca atatcaattt aaagacaagc atttgaccgt tttgcttttg
    24841 actcatgatg gcgacaaagt agcccgtttg atcaccgctt attctgaaaa agtagacaaa
    24901 aaagtggtcg ccgatcgttt agcggaatgg aaaaagacaa atgaacaagt caagaaaaca
    24961 actgtctctt ctcaaaaaaa cgagtcgaca ggaacaagtc cttcagattc tggcagtgga
    25021 caaacgataa cgggaagtga agaaaatcaa gcctcaatta ttacgaatga gtcatcacaa
    25081 ggacaatatc aatcctatgg acaatgaaaa a
//
LOCUS       DACEMS010000035        23859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEMS010000035 DACEMS010000000
VERSION     DACEMS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.035"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(196..501)
                     /locus_tag="IW735_001233"
     CDS             complement(196..501)
                     /locus_tag="IW735_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1044245.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(526..957)
                     /gene="ruvX"
                     /locus_tag="IW735_001234"
     CDS             complement(526..957)
                     /gene="ruvX"
                     /locus_tag="IW735_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1044246.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(954..1220)
                     /locus_tag="IW735_001235"
     CDS             complement(954..1220)
                     /locus_tag="IW735_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1044247.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1475..2200)
                     /locus_tag="IW735_001236"
     CDS             complement(1475..2200)
                     /locus_tag="IW735_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1044248.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2561..2631)
                     /locus_tag="IW735_001237"
     tRNA            complement(2561..2631)
                     /locus_tag="IW735_001237"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2597..2599),aa:Gly,seq:ccc)
     gene            2748..3500
                     /locus_tag="IW735_001238"
     CDS             2748..3500
                     /locus_tag="IW735_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1044249.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3516..3995
                     /locus_tag="IW735_001239"
     CDS             3516..3995
                     /locus_tag="IW735_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1044250.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4246..5826)
                     /locus_tag="IW735_001240"
     CDS             complement(4246..5826)
                     /locus_tag="IW735_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1044251.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5819..6562)
                     /locus_tag="IW735_001241"
     CDS             complement(5819..6562)
                     /locus_tag="IW735_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044252.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6738..7514)
                     /locus_tag="IW735_001242"
     CDS             complement(6738..7514)
                     /locus_tag="IW735_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1044253.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7762..8598)
                     /locus_tag="IW735_001243"
     CDS             complement(7762..8598)
                     /locus_tag="IW735_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1044254.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8609..9538)
                     /locus_tag="IW735_001244"
     CDS             complement(8609..9538)
                     /locus_tag="IW735_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1044255.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9730..10083
                     /locus_tag="IW735_001245"
     CDS             9730..10083
                     /locus_tag="IW735_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1044256.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10157..10813)
                     /locus_tag="IW735_001246"
     CDS             complement(10157..10813)
                     /locus_tag="IW735_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1044257.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            11039..12973
                     /locus_tag="IW735_001247"
     CDS             11039..12973
                     /locus_tag="IW735_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1044258.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(13059..14282)
                     /locus_tag="IW735_001248"
     CDS             complement(13059..14282)
                     /locus_tag="IW735_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1044259.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14276..16174)
                     /locus_tag="IW735_001249"
     CDS             complement(14276..16174)
                     /locus_tag="IW735_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1044260.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(16168..17286)
                     /locus_tag="IW735_001250"
     CDS             complement(16168..17286)
                     /locus_tag="IW735_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1044261.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17369..18202)
                     /locus_tag="IW735_001251"
     CDS             complement(17369..18202)
                     /locus_tag="IW735_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1044262.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18195..19112)
                     /locus_tag="IW735_001252"
     CDS             complement(18195..19112)
                     /locus_tag="IW735_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1044263.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19339..20463)
                     /locus_tag="IW735_001253"
     CDS             complement(19339..20463)
                     /locus_tag="IW735_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1044264.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20646..21554
                     /locus_tag="IW735_001254"
     CDS             20646..21554
                     /locus_tag="IW735_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1044265.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21695..23308
                     /locus_tag="IW735_001255"
     CDS             21695..23308
                     /locus_tag="IW735_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1044266.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7431 a         4655 c         3993 g         7780 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gtattgaata cttcttcaat
      241 catgtcccat tctgcgtctg tttcgatttg ttctaactcg ccttctgttc catcttcgtt
      301 ttcgatgtat gcataagctt gtaattctac atcttcatct tctgaaacac cagcaggata
      361 taaaagaaca tagttacgtc caaattcttc ttgtccgtca actgttagta ggatttcata
      421 taatgtttca tttccttggt catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg
      481 ggaatggtta tgttctgtca taagattacc tcttttcttt tattattaaa ataattgctt
      541 acctgagccg tctaaataat tttgtaagat catcacagct gctaatttat cgatgacttt
      601 ttttcgtttg gctcttgaag tatttgcctg ctctactagc atacgttctg cctgaacagt
      661 ggttaatcgt tcatcttgat aatcaactgg caaggagaat agttctttga tcttttctcc
      721 gtatgacatg gaagcttctg cccttggtcc gatggaattg ttcatatttt taggtaatcc
      781 aactacaaac cggtctactt catattgtgc tactaattcc ttcaaccgat caaagccaaa
      841 ctcttcttct gcttcattga tccgaatgat ctctactcct tgagctgtcc agccaaaggg
      901 gtcactgata gcaacaccta ctgtcttaga accgacatcc aagcccatta ctctcataaa
      961 ttcactccat gatttgagag gtagtccttg acaattactt ccatgatctc atcgcgttcg
     1021 tgacggcgaa tcaaatttcg agcatcacga taacgaggaa tgtaggccgg gtctccagaa
     1081 agcaagtagc cgacaatctg attgattgga ttgtagcctt tttcttccaa agcccgataa
     1141 acaatcgcta atgtttcgct cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg
     1201 gtttcatctg taaaacccat gattctacac ctctttcccg caaggtaatt ccagcttacc
     1261 ttctacaacc ttattttact cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc
     1321 gaataaatta agaaacacta tttgtatgtt tttttccttt tttctgtagc tttatcaaaa
     1381 gtactctttt cataaagtat acgctatatt tctttgaatg tatgttttta cacaattttt
     1441 tcataatatt cataaaaaat tcgaatagct gctactaatt ttttgttttt gttgcttgtc
     1501 ctgtctcata attgatcgtg tagccatctt gaacattgta gataaagaca ttgaattcaa
     1561 tttttttgtc ttcaatgctt tctgcttcca attgcactcc cctggctacc agttcattcc
     1621 cctcaaaatg aggagtgaca cggtagcgta catgatgatt tgtttctttg atatatgccg
     1681 caatatcatt ttcgtgagca agcatgtatg gagcattcaa cgaacgtgtc cctgtcatca
     1741 agtttttaat gttgttattt tgaccagtca gctggaaacc aattaaatgg ctgcgattat
     1801 ataaccattg cccgtcactt aatttctttt gtttccaacc tgtcgggtca atatacaaag
     1861 gctctctctt ttcagtaggg aacaaatctt ttccaagcat agcattggct tgtcctactc
     1921 tgttcaattt atcaatattt gaatagcttt cccatgttcc tttggaaagg ctaagatcct
     1981 cttttgtaaa ttccggctga ttcccattga tttctatttc ttgatgtttt ccatcatatt
     2041 ctggcacatc cgctacttga gaagcttgtg gtgtggaatc aaaaatagtt gtaatctgct
     2101 gaaagatatc tgaatctttt tgtgtggtgg aaacaccgaa tgcccctgca atcacagcag
     2161 cgacgatccc cacgatcgcc ttggttgttt ttttgctcat ttctttactc ctttactaac
     2221 agctccctaa atcttaacat atgttcccct ataaataaat actagaaaga aatagaaaca
     2281 gcaaaaaata aaacctgttt taagatggct taaaaataca aaaaagagac ggattcctcg
     2341 tctctaatca gtcaccactc tatctgtaag gtatttatta ttattcaaaa attttcttca
     2401 tactcaagac aatacactga tttcacaacc gcttggtaaa cggtgcgcaa aagcttgcat
     2461 ctgcggtaat aagtccatcc aagttaaaaa cataagaagt tgtttttgct tggctgttct
     2521 tattacttgt tgcagagcag ctttttcaca acctatttta agcgggtgac gggaatcgaa
     2581 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg caatacaaaa
     2641 acagtatacg cctcttttat tccttcctca agttttttta ttctttacgc ttctggtatg
     2701 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     2761 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     2821 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     2881 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     2941 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     3001 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     3061 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     3121 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     3181 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     3241 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     3301 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     3361 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     3421 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     3481 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     3541 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     3601 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     3661 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     3721 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     3781 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     3841 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     3901 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     3961 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     4021 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     4081 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     4141 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     4201 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     4261 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     4321 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     4381 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     4441 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     4501 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     4561 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     4621 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     4681 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     4741 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     4801 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     4861 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     4921 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     4981 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     5041 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     5101 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     5161 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     5221 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
     5281 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
     5341 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
     5401 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
     5461 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
     5521 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
     5581 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
     5641 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
     5701 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
     5761 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
     5821 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
     5881 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
     5941 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
     6001 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
     6061 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
     6121 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
     6181 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
     6241 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
     6301 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
     6361 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
     6421 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
     6481 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
     6541 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
     6601 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
     6661 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
     6721 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
     6781 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
     6841 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
     6901 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
     6961 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
     7021 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
     7081 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
     7141 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
     7201 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
     7261 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
     7321 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
     7381 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
     7441 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
     7501 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
     7561 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
     7621 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
     7681 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
     7741 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
     7801 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
     7861 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
     7921 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
     7981 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
     8041 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
     8101 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
     8161 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
     8221 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
     8281 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
     8341 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
     8401 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
     8461 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
     8521 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
     8581 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
     8641 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
     8701 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
     8761 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
     8821 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
     8881 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
     8941 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
     9001 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
     9061 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
     9121 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
     9181 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
     9241 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
     9301 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
     9361 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
     9421 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
     9481 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
     9541 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
     9601 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
     9661 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
     9721 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
     9781 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
     9841 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
     9901 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
     9961 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    10021 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    10081 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    10141 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    10201 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    10261 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    10321 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    10381 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    10441 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    10501 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    10561 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    10621 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    10681 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    10741 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    10801 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    10861 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    10921 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    10981 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    11041 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    11101 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    11161 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    11221 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    11281 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    11341 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    11401 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    11461 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    11521 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    11581 acaccctgac attttgattc tagatgaacc aacgaaccat ttagatatcg agacactttc
    11641 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    11701 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    11761 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    11821 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    11881 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    11941 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    12001 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    12061 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    12121 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    12181 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    12241 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    12301 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    12361 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    12421 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    12481 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    12541 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    12601 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    12661 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    12721 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    12781 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    12841 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    12901 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    12961 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    13021 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    13081 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    13141 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    13201 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    13261 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    13321 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    13381 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    13441 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    13501 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    13561 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    13621 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    13681 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    13741 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    13801 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    13861 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    13921 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    13981 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    14041 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    14101 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    14161 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    14221 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    14281 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    14341 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    14401 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    14461 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    14521 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    14581 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    14641 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    14701 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    14761 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    14821 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    14881 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    14941 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    15001 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    15061 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    15121 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    15181 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    15241 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    15301 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    15361 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    15421 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    15481 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    15541 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    15601 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    15661 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    15721 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    15781 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    15841 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    15901 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    15961 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    16021 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    16081 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    16141 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    16201 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    16261 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    16321 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    16381 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    16441 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    16501 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    16561 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    16621 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    16681 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    16741 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    16801 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    16861 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    16921 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    16981 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    17041 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    17101 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    17161 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    17221 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    17281 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    17341 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    17401 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    17461 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    17521 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    17581 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    17641 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    17701 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    17761 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    17821 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    17881 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    17941 tggctgtagc taaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    18001 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    18061 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    18121 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    18181 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    18241 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    18301 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    18361 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    18421 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    18481 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    18541 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    18601 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    18661 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    18721 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    18781 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    18841 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    18901 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    18961 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    19021 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    19081 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    19141 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    19201 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    19261 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    19321 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    19381 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    19441 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    19501 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    19561 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    19621 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    19681 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    19741 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    19801 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    19861 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    19921 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    19981 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    20041 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    20101 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    20161 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    20221 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    20281 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    20341 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    20401 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    20461 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    20521 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    20581 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    20641 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    20701 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    20761 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    20821 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    20881 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    20941 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    21001 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    21061 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    21121 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    21181 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    21241 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg
    21301 aacaattagc ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag
    21361 tgaatggaaa aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc
    21421 tcgtgctaga aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga
    21481 tccatcattt ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc
    21541 tatccttaaa atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc
    21601 tgtctattca aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac
    21661 atagtacgat caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt
    21721 agctggcggt ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat
    21781 tatctatgaa cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg
    21841 aggtgtgatc actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa
    21901 aaacaacatc gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg
    21961 attaacagtt aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat
    22021 catctcttct ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa
    22081 cggctttgtc ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa
    22141 aaatccacaa catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa
    22201 tttcaagcat cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc
    22261 ttatgatcca gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt
    22321 tcatttagaa acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga
    22381 tggctccgtc ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga
    22441 agtggtgaaa gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca
    22501 gttacaaacc aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt
    22561 agtgactggt cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat
    22621 gctggcagat attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt
    22681 cgcaaaaatc tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc
    22741 atcaggcata acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta
    22801 tccagaagct acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt
    22861 aggtggacaa gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc
    22921 aatcaccgga aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc
    22981 gtattctact acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac
    23041 gaatatcgta gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt
    23101 ctttttagat atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa
    23161 tattccaatg aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta
    23221 cattactttc agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg
    23281 tatcaaagcg attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag
    23341 gagttatttt tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt
    23401 atatagaata gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt
    23461 atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa
    23521 aaaaacatgt tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta
    23581 cgcctaatat tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc
    23641 tttcgaatat tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc
    23701 gactggcttg cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta
    23761 atcatatttt tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt
    23821 gtattaaata atttaatttt tttgttccta tttatttaa
//
LOCUS       DACEMS010000036        23657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEMS010000036 DACEMS010000000
VERSION     DACEMS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.036"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            255..530
                     /locus_tag="IW735_001256"
     CDS             255..530
                     /locus_tag="IW735_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044267.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2621
                     /locus_tag="IW735_001257"
     CDS             543..2621
                     /locus_tag="IW735_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1044268.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2781..4667
                     /locus_tag="IW735_001258"
     CDS             2781..4667
                     /locus_tag="IW735_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1044269.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IW735_001259"
     CDS             4679..5626
                     /locus_tag="IW735_001259"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1044270.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IW735_001260"
     CDS             5646..6173
                     /locus_tag="IW735_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1044271.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IW735_001261"
     CDS             complement(6236..6889)
                     /locus_tag="IW735_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1044272.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IW735_001262"
     CDS             7021..7863
                     /locus_tag="IW735_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1044273.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IW735_001263"
     CDS             8021..8917
                     /locus_tag="IW735_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1044274.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IW735_001264"
     CDS             8920..9633
                     /locus_tag="IW735_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1044275.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IW735_001265"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IW735_001265"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1044276.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IW735_001266"
     CDS             10168..10395
                     /locus_tag="IW735_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1044277.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IW735_001267"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IW735_001267"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1044278.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IW735_001268"
     CDS             complement(11181..11723)
                     /locus_tag="IW735_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1044279.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IW735_001269"
     CDS             complement(11960..12358)
                     /locus_tag="IW735_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1044280.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IW735_001270"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IW735_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1044281.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IW735_001271"
     CDS             13471..14238
                     /locus_tag="IW735_001271"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1044282.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IW735_001272"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IW735_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1044283.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IW735_001273"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IW735_001273"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1044284.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IW735_001274"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IW735_001274"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1044285.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18894..19799
                     /gene="xerC"
                     /locus_tag="IW735_001275"
     CDS             18894..19799
                     /gene="xerC"
                     /locus_tag="IW735_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1044286.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19836..20384
                     /gene="hslV"
                     /locus_tag="IW735_001276"
     CDS             19836..20384
                     /gene="hslV"
                     /locus_tag="IW735_001276"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1044287.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20398..21798
                     /gene="hslU"
                     /locus_tag="IW735_001277"
     CDS             20398..21798
                     /gene="hslU"
                     /locus_tag="IW735_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1044288.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21816..22607
                     /gene="codY"
                     /locus_tag="IW735_001278"
     CDS             21816..22607
                     /gene="codY"
                     /locus_tag="IW735_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1044289.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22689..23564
                     /locus_tag="IW735_001279"
     CDS             22689..23564
                     /locus_tag="IW735_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1044290.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4079 c         4983 g         6403 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcattttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacaccggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt cttgcgtttt tcttaagaat ttctcaaaca aaatgtgagg
    18781 aattgcgcca attgtgaaca aacttagaaa tattggttaa attctgacaa tttatttgtc
    18841 tttttataaa cgctgtgcta aattattctc tgggacaaaa ggaaggtgag aaaatgacag
    18901 aagccacgtg ggaacaaaaa tttttgcgtt atttgattgt cgaaagagga tattcagaaa
    18961 aaacgaaaga agcatatgaa gaagacattc tccatttcaa acattttttg gaggaatctg
    19021 gagacgctga tctgctgaag gcggaatatt ttacggttcg tgtctatttg agtgcattag
    19081 ctgatcatcg tcctagctat agcagaaatt ctatcagtcg caagatagcc agcctacgtt
    19141 cgttttatca gtatcttctc aaagaagaag tgataaaaga aaatccattt tcttacgttc
    19201 atctcaaaaa gaaaaatttg agactgccac gtttttttta tgaaaatgaa atgcaagcac
    19261 tttttgacag cgtagcagga gatagtcctt tggatttgag gaaccgcgct ttactggaag
    19321 tcctttacgg gtcaggcatc cgtttaagtg agtgtagcag tttgtcggta tcagacattg
    19381 attttgattc agaggtcatg ttgatccatg gtaaagggaa caaagagaga tatgctcctc
    19441 taggttcttt tgctcaagat gctctgcaag aatatttcag ggaaggccgc caggtattga
    19501 tgacaaaata tcacgaagag catgatatcg tttttgtgaa tcatcatgga aaaccaatca
    19561 cgcctactgg aattgaatat gtcttgaatc aaatgatcaa aaaaagcagt ttggatagtg
    19621 atatccatcc tcacatgctg cgccatacat ttgctactca tttattaaat aatggggcag
    19681 atttgcggac agtccaagaa ttgctaggac atgccaattt atcgactacc cagatctatg
    19741 cccatgtaac aaaagaaagt ttacaaaaaa actatcgttc tttccatcca agagcgtaaa
    19801 aataaggaaa agaacaaatt taggaggaaa caaacatggt cgaatcacca tttcattcaa
    19861 ccactatctg tgccgtcgaa aaagacggga aatttgctat ggctggagat ggccaagtaa
    19921 caatgggtga atctgttgtc atgaaaggca ctgcaaagaa agtacgacgt atctacaatg
    19981 gagaagttgt tgtaggtttc gccggaagtg ttgcagatgc gtttacttta gaagaaaaat
    20041 ttgaaggcaa actaaacgaa tataacggga atctacaacg cgcagctgtc gaattagctc
    20101 aagaatggcg gacacagcaa tctatgcaaa aacttgaagc gatgctgatc gtaatgaatg
    20161 ataaagaaat gcttcttgtt tcaggtacag gagaagttat cacaccagat gatggtatct
    20221 tagctatcgg atcaggaggg aattttgcgt tggctgcagc ccgagcaatg aaaaactatg
    20281 ctcaaaacga catgagtgcg aaagaaattg cagaaaatgc gttaaatatt gccgcagata
    20341 tctgtatctt tactaatcat aatattatcg tagaagaact gtaagaggtg agtagatatg
    20401 tatgaattaa aacaaacacc aagacaaatc gtaagtgaat tagatgaata tatcgtagga
    20461 cagcacgcag caaaaaaatc tgttgccgtt gctttgcgta accgctatcg ccgtttgcag
    20521 ttagatgaaa aaatgcaaca agatattaca ccaaaaaata tgctgatgat cggacctaca
    20581 ggggtcggta aaacagaaat cgcacgacgt ttggcaaaaa tcgtcaatgc accatttgtc
    20641 aaagtcgaag caacaaaatt tactgaagtc ggttatgtcg gaagagatgt agaatcaatg
    20701 gttcgggatt tagtagaaaa tgcgatccaa atcgtagaaa aagagcagta cagccgtgta
    20761 tacacaagtg ctaaaaaaaa tgcagaaaaa cgtttggtaa aaatcttggt acctggtatc
    20821 aaaaaagaac agaaacaatc cactaatcaa tttgaacaaa tgatgaatct ttttaatgct
    20881 agtcagcaaa ataccgctga accgcaagaa gaagtgacag aagatattcg aatcaatcgc
    20941 cgtacagtct tggaacaact acaaaagggt ttactggata gtcgtgaagt aaccattgaa
    21001 gttgaagacc aaaagaaacc tgcaccaatg atgaacaatg gattggagca aatggggatc
    21061 gacctaaatg aaactctagg tgctttgaca ccgaagaaaa aagtggaacg gacggtaaca
    21121 gtcaaagaag ctttagagtt attgatcaag gaagaatcag caaaattagt caaggatgct
    21181 gatatccata gtgaggcaat caaattagcg gaagaaagtg gaattatttt tatcgatgaa
    21241 atcgataaga tcacttctaa atcacagcaa tctggagaag tatccagaga aggcgtccaa
    21301 agagacatat tgccaattgt cgaaggttca caagtaaaca caaaatacgg gatcatccaa
    21361 acagatcacg tcctattcat tgcttcagga gctttccatt tgtctaaacc aagtgatcta
    21421 attccagaac ttcaaggacg cttcccgata cgggtagaat tagatgattt gacagctgaa
    21481 gattttgtga agattctgac cgaaccaaat aatgctttag taaaacaata cgtcgcgttg
    21541 atcggtacag aaaatgtgtc agttacgttt acaaaagaag caattgaacg gatcgctcaa
    21601 atcgctttcg atgtcaaccg tgacacagat aatatcggcg cacgcagact gcataccatt
    21661 ttggaaaaac tactagaaga tctattattt gaagcaccag atatgcaaat gggagaaata
    21721 acgatcacag aagcatatgt ggatgacaaa cttggctcaa ttgcggcaaa tgaagattta
    21781 agtcgctata ttttataaaa aatcggagga cgacaatgac agacttgcta acgaaaaccc
    21841 gcaagataaa cacacttttg cagcaaaaga atacttttga catgcaagca gatttgcctt
    21901 acgataaaat ggctgtcaca ctaggtggta ttcttgacag caacacctat atcataggaa
    21961 gtgacggagt attactaggt tataatgaac gtcacgatgt gaacaatgaa cgaatcaaaa
    22021 caatgtttgt tgagaaacaa tttcctgaat cttatactga tacagtggca cagttgatga
    22081 aaactgaagc taatatccca attactagtg atattactgc atttccggta gagtcaagag
    22141 agaaatatcc atttggtctg acgactgtcg ttccgatctt tggagctggc gaacgtttgg
    22201 gtacgattat cttatctcgt atggaacaat cgtttgcaga tgacgatcta gtcttagcag
    22261 aatatggagc tacggttgtc ggtatgcaga tcctttatca gaagtctcga agcatcgaag
    22321 cagatgtacg gagtgcaacg gctgtccaaa tggcaatcaa cacattatct tatagcgaat
    22381 tgaaggctgt acaagctatc tttgaagcat tggatggaga tgaaggtcgt ctgactgctt
    22441 ctaatattgc cgatagtatc ggcatcactc gttctgtgat cgtcaatgct ttacgaaaat
    22501 tggaatcagc tggaatcatt gaatctcgtt cgttaggcat gaagggaact tatttaaaag
    22561 tattgaattc ccgttttaaa gacgagttag caaaacacag ttattagaag taaggggact
    22621 ggggcagata gaaatgtttg ttccagcctt cccattttaa aaagtaagtc gaagttggag
    22681 gaatggcaat gactgttatt attcaaaatg accagctgat cgccgaaatc gctgaacacg
    22741 gtgcggaatt gatcagtctg aaatcaaaag aacatggttt agagtatatc tggcaaggtg
    22801 atccagcgta ttggggcaga catgcaccgg ttttattccc ttttgttgga cgtttaaaag
    22861 atgatcagta tgtttatcaa gggaaaacat atcaaatggg gcaacacgga tttgctcggg
    22921 atatggattt tgaagtcatt gaacatggac aagaaatggc tagtttcatc ttaaggagta
    22981 cgccggagac attagaaaag tatccatttg attttgaatt agtgatcagc tatgaattag
    23041 gaggagacgg catcacggtt cattatcaag tggcaaatac cggtgaaaaa gaaatgttct
    23101 tttctatcgg tggacatcct gctttcaatg tacctcttga agaaggattg tcatttgaag
    23161 atttctatct ggcattttct ccattgaaat caagattacg cctaccattg aaaggcccat
    23221 tcatcgatat ggatcaaaag acgttaggtc agacaaatac gaatctggct ttgaatcgtg
    23281 aactttttga gcaagatgcc ttggtttttg aaacaagggg tctaaatgcg tacgccattc
    23341 gaagtgaaaa atcttcccat agcgtaactt taagctataa agatatgcct tatgtaggga
    23401 tctggtctcc ttatccaaaa gaagcgcctt tcgtttgtat cgaaccgtgg tgcggaatcg
    23461 ctgacacggt gaacagtagt ggaaacttga tggaaaaagt agggatcaat caactaggtg
    23521 cccatgaaat cttcaagaca aaatattcca tcacagtgaa gtaagaggtt gtgaccgaga
    23581 agttagcagg gaacaccgtt taccaaggtg tgtaaaaaag ctatcctgta agtaataagg
    23641 gagtgtgaca tcgattt
//
LOCUS       DACEMS010000037        22340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEMS010000037 DACEMS010000000
VERSION     DACEMS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.037"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            410..2245
                     /gene="tdc"
                     /locus_tag="IW735_001280"
     CDS             410..2245
                     /gene="tdc"
                     /locus_tag="IW735_001280"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1044291.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2550..4028
                     /locus_tag="IW735_001281"
     CDS             2550..4028
                     /locus_tag="IW735_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1044292.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3994..5325
                     /locus_tag="IW735_001282"
     CDS             3994..5325
                     /locus_tag="IW735_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1044293.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5439..7991
                     /locus_tag="IW735_001283"
     CDS             5439..7991
                     /locus_tag="IW735_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1044294.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7998..9437)
                     /locus_tag="IW735_001284"
     CDS             complement(7998..9437)
                     /locus_tag="IW735_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1044295.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9783..10145
                     /locus_tag="IW735_001285"
     CDS             9783..10145
                     /locus_tag="IW735_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044296.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10332..11189)
                     /locus_tag="IW735_001286"
     CDS             complement(10332..11189)
                     /locus_tag="IW735_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1044297.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11309..12220
                     /locus_tag="IW735_001287"
     CDS             11309..12220
                     /locus_tag="IW735_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1044298.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12227..13873
                     /gene="mdcA"
                     /locus_tag="IW735_001288"
     CDS             12227..13873
                     /gene="mdcA"
                     /locus_tag="IW735_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ1044299.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13975..14274
                     /locus_tag="IW735_001289"
     CDS             13975..14274
                     /locus_tag="IW735_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1044300.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14264..15940
                     /gene="mdcD"
                     /locus_tag="IW735_001290"
     CDS             14264..15940
                     /gene="mdcD"
                     /locus_tag="IW735_001290"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1044301.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15944..16537
                     /locus_tag="IW735_001291"
     CDS             15944..16537
                     /locus_tag="IW735_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1044302.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16561..17637
                     /locus_tag="IW735_001292"
     CDS             16561..17637
                     /locus_tag="IW735_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1044303.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17662..18531
                     /gene="mdcB"
                     /locus_tag="IW735_001293"
     CDS             17662..18531
                     /gene="mdcB"
                     /locus_tag="IW735_001293"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1044304.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18642..19406
                     /locus_tag="IW735_001294"
     CDS             18642..19406
                     /locus_tag="IW735_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1044305.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19895..21988
                     /locus_tag="IW735_001295"
     CDS             19895..21988
                     /locus_tag="IW735_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1044306.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            22013..22315
                     /locus_tag="IW735_001296"
     CDS             22013..22315
                     /locus_tag="IW735_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1044307.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7253 a         3799 c         4745 g         6543 t
ORIGIN      
        1 ttttgaaatc atcagttaat ttctgaaaaa gcatataaat gccgttaaaa aaggtttttt
       61 atgttataaa atttggaaaa cagataattc tattaaaaaa taaattgcga atttttaaca
      121 atataaaaaa ataaaaatat gtttagtgaa tgaaggacag tcattttatt tgtttgttcg
      181 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
      241 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      301 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      361 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      421 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      481 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      541 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      601 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      661 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      721 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      781 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      841 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      901 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      961 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
     1021 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
     1081 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
     1141 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1201 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1261 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1321 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1381 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1441 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1501 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1561 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1621 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1681 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1741 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1801 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1861 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1921 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1981 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     2041 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     2101 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     2161 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2221 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2281 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2341 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2401 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2461 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2521 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2581 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2641 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2701 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2761 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2821 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2881 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2941 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     3001 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     3061 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     3121 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3181 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3241 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3301 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3361 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3421 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3481 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3541 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3601 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3661 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3721 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3781 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3841 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3901 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3961 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     4021 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     4081 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     4141 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4201 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4261 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4321 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4381 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4441 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4501 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4561 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4621 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4681 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4741 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4801 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4861 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4921 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4981 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     5041 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     5101 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     5161 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5221 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5281 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5341 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5401 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5461 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5521 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5581 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5641 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5701 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5761 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5821 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5881 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5941 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     6001 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     6061 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     6121 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6181 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6241 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6301 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6361 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6421 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6481 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6541 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6601 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6661 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6721 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6781 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6841 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6901 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6961 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     7021 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     7081 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     7141 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7201 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7261 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7321 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7381 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7441 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7501 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7561 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7621 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7681 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7741 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7801 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7861 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7921 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7981 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     8041 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     8101 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     8161 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8221 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8281 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8341 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8401 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8461 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8521 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8581 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8641 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8701 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8761 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8821 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8881 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8941 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     9001 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     9061 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     9121 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9181 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9241 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9301 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9361 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9421 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9481 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9541 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9601 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9661 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9721 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9781 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9841 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9901 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9961 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
    10021 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
    10081 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
    10141 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10201 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10261 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10321 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10381 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10441 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10501 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10561 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10621 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10681 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10741 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10801 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10861 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10921 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10981 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    11041 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    11101 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    11161 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11221 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11281 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11341 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11401 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11461 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11521 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11581 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11641 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11701 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11761 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11821 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11881 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11941 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    12001 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    12061 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    12121 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12181 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12241 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12301 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12361 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12421 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12481 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12541 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12601 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12661 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12721 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12781 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12841 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12901 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12961 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    13021 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    13081 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    13141 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13201 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13261 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13321 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13381 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13441 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13501 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaccagt
    13561 gatgatttat agcgaagata ctacacatat cgtgacagaa gaaggaattg cttatttgta
    13621 caaagcaaaa acaaaagaag aacgacaagc agcaattgca gcaattggcg gtgttacacc
    13681 tcttggaatg actagcacga aagaatcact agatagcttg cgtgcagccg gcatcgtgaa
    13741 attaccagaa gatctaggca tcaaacgtag tgaagcgaaa cgttcattat tagctgcaca
    13801 aaccatcgat gatttgatcg aatggtcaga tggcctgtat gaaccaccaa tgaaattcag
    13861 aacttggtca taaaattggc gagaaaaacc ttttgtcgcc atttacccag atggaagaag
    13921 aaagacgaat aagcagaacg tgtatgctct gttttacaaa aaggggagag atccatggaa
    13981 caattagctt ataaatatcc aacgaaacag ccgatcaata agaaaatcca tgtaggagtg
    14041 gtcggttcag gagatttgga aatcttgatg tttccaacag aaaaaacaga atctactgta
    14101 aaaatttgta caggaagcga tggcttcggc gaagtatgga acaatgtatt aacgagattt
    14161 tttgatcgtt atccaatttc agcagatatc gtgatcaatg acttcggtgc tacaccagga
    14221 gttgtgtacc tacgattgac gcaagcaatg gaggaacttt cagatgaaaa atagttttgt
    14281 agaattaaac gccagagaac gagtgaacgc tctacttgac aatggacgtg aattgattga
    14341 tccattcgat caaatcatcg cgccaagttt accagcacaa ggaatcgtcc ctgaaagtga
    14401 tgacgggatc gttgtcagcc gtggcacgat caatggcaaa caagcagtag taatcgctat
    14461 ggaaggaaaa ttccaaggtg gcggtatcgg tgaagtttcc ggtgcaaaaa tcgttgctgc
    14521 acttgaacat gtgttagaag aaaataaaaa aggaaatgag atctttccag tcattgtact
    14581 agatacaggt ggtgttcgtt tgcaagaagc aaactacgga ctgttatcta tttcagaaat
    14641 cggaaatgta atcattgctc taaaacaata tgtaccagtc atcggtttga ttccaggacg
    14701 tgtaggatca tttggcggta tgtcgattac ttcagcatta atgagttatt taatcgcaac
    14761 aaaaaaagcg agagtcggtt tgaatggacc agaagttatt gaacaagaag caggcgttcg
    14821 agaatttgat tctagtgaca aagacttgat ttggaataca ttaggatctc gtcaacgggt
    14881 agaatcaaaa atcattgatg aattagtgac tgatagtgta gaagcaatca aagctgctgt
    14941 catcgcagca atggacgaac gaaaagacac gcatcgcagt gaaagctcag cattttattt
    15001 atcattactt gattctttag atctttcaaa accaatggcc attgacgatt acaatgaagc
    15061 aattgctaac catcaagcaa aatcaattga cttaccagaa gtagcagaag gaaatgaacc
    15121 agctgcagca agtgttggtt acgattggtt tgcggcacta actggcatga aaaatccaac
    15181 ttcttctatt agtactgtgt attatggtaa atcaagtaaa ttcatagaag aagccatcgt
    15241 gacatcaatc gtacctgatc ctaaaaaccc gatgtaccgt gtacgaaacg gtgaagttgg
    15301 tttagttgaa ggtttccgca tgggacaaat tttggatcac gtttacgaag aagataaaga
    15361 caaaaaagtg aaacggccaa tcatcgttgt gatcgatgta ccaagccaag catacggcta
    15421 taacgaagaa ttgatcggta tccatgtggc tttggctaat agtgctgctg cttatgcaaa
    15481 actccgtcaa gctggacatc cagttattgg attgattgca ggaaatgcta tttcaggtgc
    15541 atttttagca catggattac aatcaagccg cttgattgct ttaaacaata aagaaattac
    15601 cgtccaagcg atgtctaaag aaagtgctgc acgtgtgaca aaacgttcgg ttgctgaaat
    15661 cgaagcagct gctgaaaaag ttccagctat cgcttacgat atcaataatt atacaaaact
    15721 aggagcgatc tatcatttcc tagaagatat caccgaccaa gctgcttcag aacaaaatgt
    15781 cagcaaagtc atcgaagcaa tcaatgaagc cattcaagat gtccgcaata caaatgacac
    15841 gatgctgaca aatcgataca caaacgaaac agccaaaaca ttcggacgtg tcgaaacaaa
    15901 taaagtccgt gccaaaatgg acgaagaatg ggatgcataa aaaatgattc aagcacatga
    15961 tattgtcctt tttgataaac atgaaataga agggttcacc ttgcctgaat gggtcgttct
    16021 aactgaaaag atgttcggaa cagttcgtcg tgcgtcgacg gatcacgcag aaatactacc
    16081 gattggttta cgtggacctg cccgcaatca gagatggtca acagaaatct caaaacatgc
    16141 cgtcaaacaa gtcattcatc cgtgggacat catcgaacaa gaatcgtata gacaagagaa
    16201 aatcatggat tatcctgttt atcagcaatt tcatgctgct agaaacatat tatcaggatg
    16261 taaatgggga atcggcggca gcttaggatt tgaattatcg actgggattc cggcagtaaa
    16321 agaaaccagt gattttgatt tgctgctgta cgcaaattca ccgattgaat tgccaattca
    16381 ggtaattcaa tcgcatcctg ctttttttga gcaattcgat acccaagtag tgactcggca
    16441 aggcggtttt tcactaaaag agtatttgag agagccagag aaaaaaattc tattgaaaac
    16501 agtcgaagga cctaaattaa ccaaagaact ttggtaataa agaaaaagag agagaaagag
    16561 atgtttgaaa acaaaaaaat caaaatcaac gtaagtgact tccagaaagt atcagctgta
    16621 acggctgtta tgatgcaaac gatattgagc tttgctttgg caaacagcca cagcgcaaca
    16681 aatacagctt ttcttggtac cttttacgtc atcgttaaat acacagcacc aatgtttatt
    16741 tttgcaattg tttataatat ggtaaaaaca agtcagcatt tgtcttattg ggaatttttg
    16801 aaagaaaagt tttttgaact agtcgttccg tatcttctat ggacgacggc ttatctttgg
    16861 ctatttccag cagtccaaca acaaactccg tatacggata ctgcaagctt tttactgaaa
    16921 tatgtcacag gtgatggagc gcctcattta tggtataccg ttatgatgct gcagatccaa
    16981 ttgttcatgc cgttttttgt ttggctcggt tacaaagtat ttgctaataa aaaatatgta
    17041 tggccagtat tgattggttc aacgatttta tatgtcgcat ggtatctgtt ctatcaaaca
    17101 caagtattga ctgggcctta tcatgacagc tggtacttat tagatcggtt tgttgctagt
    17161 ttcatgattt atggggtagc cgcatttgtt tatcacgaaa aagtctatca atatttggac
    17221 agagtacgtt atcttttctt acctgttgcc ttagttatcg cgttcttttc tgtccgttca
    17281 ttgctcgcac atccaggaga cttgagcttt gcgaatgcac cttacttgaa tacgatccaa
    17341 agtttataca gtttagtcat catctttgct gtcttcatag gcgcttccaa aatgattgta
    17401 aacgattcgc caaaattgcc attgttcaaa tggttgtctg tttatgctta tcgtacgtat
    17461 ttagccaatg tatttgtttt ccaagtactg ttattattgt ttaaagatag ttggctgcag
    17521 ttaccttcag gagtcatgat tcttgtagct tatctaatga cggcaagttg tgcatttgct
    17581 ttaagctggt tacttcatat catttgggta gctatcaaaa aaggtttcag caagtaatag
    17641 taggaagaag gagtgagtgt catgataaat gacgaacgaa aaaaagcagt gactcagcaa
    17701 ctcgctgcgt tggtagagga agcattgatc gcagaagtaa cattaagtcc aaaacctgga
    17761 ttagtggatg cactggatgc gggtgcacat tccgatatgg actatgcttt atttcttaag
    17821 tctgctaaga cgctaactcc tttttttgaa gaaatggctc aaatagcatg gcatcatgct
    17881 attgatcagg agttaagaga aagaattgct gagattgggc gaaaagcgga aaaggcaatg
    17941 ctgattgcta caaatggcat caatacccat aaaggagcga tttgggcaat ggggctattg
    18001 atcagtgtca ttgcacaaaa aatcagtatg gaacagcctt tttctttatc gaaaatactt
    18061 gatgatgtca gcaagcttgc tgcttttcct gatacaaaat atcgtcataa aaaaaccact
    18121 catggtcata gggtgaagca aaaatatggc gtaaatggtg catatgaaga agcgatatta
    18181 gggtatccgc atatcaggct tgcaattcaa gcagctaagg agctagataa tgattcatat
    18241 gtacagaaac aaatgcatat gttgttagtt ttgatgagtt cgctagatga cacatgcgta
    18301 ttgcatcgca gtgatcagaa aacgttagtg gaaatgcaaa aacttgctca tgctgctagt
    18361 caaagtgctt taccaaatcg tccatttaca gcactgttgc acttttgtca ggaaaaccaa
    18421 atctctcctg gaggaagcgc agatctcttg tctgcaagct tgttcatgct gaagatagag
    18481 gaattatgga ctgccgacac atacatttgt ccgatgttac tcaatgacta aataagacaa
    18541 aattgctaaa aaaacagttt atgcatctgg ttattgactc ttccttttag caaggctatc
    18601 ataaaaatag ttttttgtaa taaaagataa cgaggaagac aatgaaaaaa atcaattatt
    18661 tagcaatcat tttgttagtg ggcctatgtt tgtttgctct gccgacccaa ggattcgctg
    18721 tcgcgggtgg tcatggtgga ggtacatcta gcggtcgaag ttcgggcaga ggttcatttg
    18781 gtggtcgttc atatgggggc agttattttg gcagtcgttc ttacggcaga gattcttatg
    18841 ggtattatgg tccaggtaca ggtccttcta gttatgagca aacgggattt cttgcattag
    18901 ctgttttgtc agtaggaggg gtttctgctt tttctatcgt caagaataaa agacaaaaga
    18961 aaaggctgaa agcggagata ttgtcccgca tgtcaggaac ggctaaagaa aagaaagcac
    19021 agtttctgga aatccaaaag atattcatga agatccaatc cgcttgggaa gaagaaactc
    19081 ctgaaaaagc cagaagctgt tatacagaca agctgtttgc cgaacatcag aaagttctgc
    19141 aaaaaaacaa agcagacggt gtaagaaatc atactaaaaa aataactatt caggaagtag
    19201 gaaattatcg acagatccac gaaaatagtt ttgccatccg aattgatttc agatgctgtg
    19261 attattcagt cgatcgaaaa gatgggcgat tgatctcagg aagtaagaca caacgacagt
    19321 atttttctca gatatggtat tttgattttt ctgagaaaga aaaaagatgg caagccgatt
    19381 ttattcagcc gattatgctg gattgacagg aaatatttat gaacggagtt gtgacaaaag
    19441 ccttgtcgca gctccttttt ctgaataaac ggtgtgccag agatagccta tcggcaataa
    19501 ggcacgattt tgtaaaatat gggaagcgat ttttcaaaat cgcttcttat cgctcaaggc
    19561 tgaacatctc tgtcacaacc tctccactaa atggtgttta aaagcttgca tctgcgataa
    19621 taagaacaac caaacgaaaa ataggaagca ctattttagt ttagttgttc ttattacttg
    19681 atgcaggaca gctttttcac aacctctttt ttgtatagat gggcaaaatt tttgtaataa
    19741 taggcgtatg tctttttcgt tgaggagatg aaaaagcata tggcttattt tttgtttgct
    19801 ttgcttactt tctttccact tcaagtggca gaaatatcta ctttttatta tgtaagcgga
    19861 accatatact tgaaatcaga aggagatcat ttctatgaaa acaagaacaa ttgaaatact
    19921 tcacagaatc atgacagccc aatatccagt aaaagcaaaa catctagcaa gtgagttcaa
    19981 tgtcagtcag cggacgatcc gtcaggaagt tcttgaagcg aatatctggt tgagcagtaa
    20041 aaagcttccg gagatccgta caattcgcaa caaaggtttt ttgttgaagc tggaaaagga
    20101 aaagcaagct cgattagaaa ctgaaataaa agatgtgaaa gacggctttt tgaatcggcg
    20161 cgaacgtatt tttgatctag ttttagccgt tgcttacagc cgaatcccga tccatctaaa
    20221 caaaaaagaa gaagaatttc aaatctctaa aagtacatta gatgaagata tgagacggtt
    20281 acgagcaaca ctggtcagtt atgggataga gatcgtcagt tatggaaagc aaggactggt
    20341 ttataaaggt tcagaacgat ctatccgtac gatgatctat gattttatca atcagaatct
    20401 gggaatgacc gacttcttta tgaatcaaga tgcacaatta acaccggctc aaaaaatctt
    20461 tctccattat tttcctttgg tggaaatcaa aaaaatacag gagatctatt ccaataagtt
    20521 gttgaaacaa gaagatgata tttataaaaa acaaatcctg ctttttacat tgatctggat
    20581 ccagcgaatc aggaaacatg aatttatctc tgccattaat tggaaaaata cagaaataaa
    20641 agacagtagc ttttctgcat ttattgacca catgatcgag gaatttcaat tggacaatgt
    20701 cccgcaagta gaaagaaatt atcttcgttt cactattgag acgttcaatg ctagagatat
    20761 ctccaattca atcgagtggg tacaagctca attactaacg atccaattga tccaatttgt
    20821 agagaacgag acacacgtac ctttccatct aaaggaagaa gctttattcg aaaatctcta
    20881 taagcacatg gctgcactga ttgtaagaat caagaatcat atccaagtca tgaatccatt
    20941 gaaagaaaac atccgaacaa attatttccc aatctaccgc gcagtcactc attttgttcc
    21001 tacgatcgaa gaagtaattg gtggcaagat actggaggac gagatagctt ttcttgtcat
    21061 ccacttttcg acgatcgcca gtgtaatcaa gcgagacttg aattatgttt ataaatcagt
    21121 cgttgtctgt aaccatggga tggctactgg aaatctgtta gcagagaatc tgaaagaaaa
    21181 gtttcctcag atcgagatta ctgcagtttt aagttcaaaa gaaatcgaat tagtagataa
    21241 attggatgtc aatctggttt tcagcacctt tcaattgaat tatcgaaata agccgctttt
    21301 agttgtcgat ccgattttaa ctgagacgaa tatcttggtt atagaagact ttttgaaaag
    21361 aaatgaagaa ctacagcggt tagagcctaa cagtcatgaa tcgacggagt tattcactca
    21421 aataatagat gtcatgaaag aaagtggtgg ccgagtagat cgaatcatct atggcaagtt
    21481 agaaaaatta ttcgaaacaa acaatttaga aatcaacaaa agggagatcc agcctatgtt
    21541 aaaagatatt ttgacggaca gcaatatcat gatcaaagaa aaagccacta cttgggagga
    21601 atcgattgaa ctagctgcta gaccattggt taaagaaaac attgtcgaag agcgctatgt
    21661 cgaagcgatg attcgtgcag tgaatgatta cggaccgtat atcgtgattg gcaaacacat
    21721 ggcactagcc catgcccgac cagaagatgg ggtcaataaa ttaggagtca gcgtagctac
    21781 gattgagcaa ccaatcgatt tcggtaatcc tgaaatggat ccagtaaaga ttattttttg
    21841 tttggcagca gtagactcct actcgcatct gaacatcatg aaagaactaa ttgaattgat
    21901 caacaacgaa gagaagttgg atcgattgat tgaatgcaca aatatcgaag atttcaaaca
    21961 actgcttttt tcagtagttg agaaataata aatgatacgg gggagaacaa agatgaacaa
    22021 attgaacatt ttattcgtat gcggtgcagg tttaggaagt agttttgcag cacagatgtc
    22081 tgctgaggat gttttgaaca aattaggagt agaagccaaa ctggaccatt gtgatatttc
    22141 ttccgcagtt tccatgaaac cagatgtgat catcacagca gaaaattttc gttcgcagtt
    22201 cgaaaagttc gcagttgatc ctaaaacggc gatgatctat ttgaaaaata ttgtttcaaa
    22261 aaatgaaatt gaagaaaaaa tcaccccagt tctgcaagat aagggagtta tctaaataca
    22321 aaaagaaaag agggtaaaaa
//
LOCUS       DACEMS010000038        22217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEMS010000038 DACEMS010000000
VERSION     DACEMS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.038"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..177
                     /locus_tag="IW735_001297"
     CDS             <1..177
                     /locus_tag="IW735_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1044308.1"
                     /translation="ILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEI
                     FLSYVQEAFYSSLI"
     gene            complement(248..1558)
                     /locus_tag="IW735_001298"
     CDS             complement(248..1558)
                     /locus_tag="IW735_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1044309.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1570..2988)
                     /locus_tag="IW735_001299"
     CDS             complement(1570..2988)
                     /locus_tag="IW735_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1044310.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3228..4796)
                     /locus_tag="IW735_001300"
     CDS             complement(3228..4796)
                     /locus_tag="IW735_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1044311.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4800..5081)
                     /locus_tag="IW735_001301"
     CDS             complement(4800..5081)
                     /locus_tag="IW735_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1044312.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5078..6001)
                     /locus_tag="IW735_001302"
     CDS             complement(5078..6001)
                     /locus_tag="IW735_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1044313.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5998..6876)
                     /locus_tag="IW735_001303"
     CDS             complement(5998..6876)
                     /locus_tag="IW735_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1044314.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6863..8200)
                     /locus_tag="IW735_001304"
     CDS             complement(6863..8200)
                     /locus_tag="IW735_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1044315.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8203..9306)
                     /locus_tag="IW735_001305"
     CDS             complement(8203..9306)
                     /locus_tag="IW735_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1044316.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9380..10888)
                     /locus_tag="IW735_001306"
     CDS             complement(9380..10888)
                     /locus_tag="IW735_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1044317.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10854..11546)
                     /locus_tag="IW735_001307"
     CDS             complement(10854..11546)
                     /locus_tag="IW735_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1044318.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11568..12542)
                     /locus_tag="IW735_001308"
     CDS             complement(11568..12542)
                     /locus_tag="IW735_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1044319.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12842..13714
                     /locus_tag="IW735_001309"
     CDS             12842..13714
                     /locus_tag="IW735_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1044320.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14296..16494)
                     /locus_tag="IW735_001310"
     CDS             complement(14296..16494)
                     /locus_tag="IW735_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1044321.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16726..17718
                     /locus_tag="IW735_001311"
     CDS             16726..17718
                     /locus_tag="IW735_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1044322.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17778..19199
                     /locus_tag="IW735_001312"
     CDS             17778..19199
                     /locus_tag="IW735_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1044323.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19192..20691
                     /locus_tag="IW735_001313"
     CDS             19192..20691
                     /locus_tag="IW735_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1044324.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20730..22124
                     /gene="uxaC"
                     /locus_tag="IW735_001314"
     CDS             20730..22124
                     /gene="uxaC"
                     /locus_tag="IW735_001314"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1044325.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6739 a         4525 c         3851 g         7102 t
ORIGIN      
        1 attctgcgtc gtaatggact gccgaaatca atggatttta gaaaagtgaa tcagacattt
       61 atttccagtg tcagcaatca acgtaatcat attccaagaa aatcattgaa ttacagaaca
      121 ccaatcgaaa tatttttgag ctatgtacaa gaagcatttt attctagctt aatttgacaa
      181 atcatatatc attaaaaaaa ataaaggtca agctaaaata ttatttaagc ttgaccttat
      241 tataatttca acgattgtca aaagtattgt ttgcagctgt gttgccagtt tctatagata
      301 aatcacagct tttgaaacat aagttttgta ctgcttcgat tcgcccgaat tcttttgcag
      361 ttatttggca atgagaaaag cagacatctt ctataggctt ttcaggaaat gctactaaat
      421 caaaagctcg ggaaggaagt gagtaacctg gagaaaggca tgcctgtacg tttttgatgt
      481 gtatgtcttt gactttcgtc atttgctttg attcaggtat ttcttcagcc actgcttgcc
      541 aactttctgg catttgagca aggtcattgc tattttttct attgtattca ttatgccagt
      601 ccattatcca agaaaaagga aattgtacat caatcatatt cagattttct accctgatat
      661 tttcgatcac tccccctctt tcttttgaag atttcattcg aataccacag tctgactgaa
      721 aaaaattgat atcgtgaata tatacgtcac taactccagc actaacttcg ctaccaatag
      781 ttacaccgta tcctgaacgg attttacatc tagcaacctc gattcttgat gattttcgat
      841 tgactcttgc accattgcca tctctgccag acttaatagc aatacaatca tcaccacagg
      901 ccaaatcaca ttcatagaca cgcacatttg ttgaagaatc aatatcaatc ccatctgtac
      961 ttggtccatc attgttgcgg atgattactt tttctactaa tacatcgtta gaataagtga
     1021 tttgcaagtt ccaaaaacct gatttctgaa aaatcaaatc acgaacaaca acatgttctg
     1081 catgatacag taaacaagcg cgtggacgtt taatagcata atccgcaatc caacgaagtc
     1141 ccttttgatc gtattcagct cttgtcccgc ttttttgatc ttttccccaa tacagctccc
     1201 accaatggtc tccttgaccg tcgattattc cttttccttc tataagaata tctttcgccg
     1261 agagaacatt caaaatcgct gctggccatt tcatttctac gccggctact cgtgtatcga
     1321 tttctggaaa tgcttcgata tctgttctcc ccttgaggac tgcaccttct tcaaagataa
     1381 gatgactctt atcttttaaa aaaagggctc cgactaaata aatgcctttt ggaataaata
     1441 caggtaagtg ttctttcgca ccttgatcga ttgctttttg cagtattatt gtatcatctt
     1501 gttgagtgtc gtttgtttta aagttcaaag cattgataat cccttttttt gtccgcaata
     1561 cgtttctccc tatctttcat tgattttttt caattcttca ccaataaata cagtttttga
     1621 acgattcagt gggtaaccta ctagcaaaat gattgcggat acaaataagc agaatgctgg
     1681 taacaagttt gccatcgtat aaatcctttc ttgtactaaa actgattgga tggctccacc
     1741 tgttgtagac gattggtaac caatgagtgc cagcataaat ccgcctaccg ctcctgcaca
     1801 tgcttgtcct acttttctcg caaaagaatt tactccatat actgttccat cttctcttaa
     1861 gcctgtctta tattgatgat aatcaatcac gtcagtaatg aatgcccaga ccatcaaatt
     1921 gaataaacca gatcctaaag tagcgataaa taaaagaatc agatataccc atgcatttgt
     1981 tatatgaagg aaatacatca gcaagtacat acatgaagaa gcaagtaacg caaatacgct
     2041 tgcttccttt ttaccaaata attttgtcag ctttgtagca aatggtgcta aaactactac
     2101 tgttccgtaa gtaaataaaa gagcaacaga cattgcttgt ttattatgga agtaatcatt
     2161 gaataaataa gtcatattcg tcccagctaa aatctgattg atcacaatga aaatgtcaac
     2221 aatgactaaa ataatcagtg ctcgattagc taacaaacct tttaccaact gagaagcaga
     2281 tactttttcg tttttgacaa ctcttactct ttcagtagtc aatttacaag ttaacatata
     2341 tgcgacaaat gctattgcag cacatccgat tgaaatccaa aagaaatgat tccctgaaac
     2401 gacttgttgt ccactcttgt tatgggtata cattaaaatc ggaatgaaga atcctgtagt
     2461 tgatccgcca atcgcagaac caatgcttcg gaacgtggac aacgaggtgc ggtcattagg
     2521 atttgagcta attgctgctg ccattgctcc atacgggata ttgattgttg agagaaaaat
     2581 cccataaaca atgtatgaaa caaaaatata agcaatcttc accgtcattg gaagatgttg
     2641 gacgaatggc aaaaacaaaa tgacagtcac taagcaaaat ggatacttca tttttctgat
     2701 ccaaggaata aatcggccag tttctgttaa ctctgagaca tcacataatc ttccaaccat
     2761 cacatcagca aacgcatcca aaattctcga aatcaagaat aacgtaccta cgattgcccc
     2821 tgaaacgcca agaacattcg tataataaat catcaagaaa ctattgacta aacctaaaat
     2881 aaaacagtta cctagatcac cgaacatgta gcctatctta tcggaaatcc caaattcctt
     2941 tttttctttt tttactgtat ttgctgtaac agcattttgt gaaagcattt gaattcctcc
     3001 tacctcttct agtaaacgtt ttcaaaatta taactctttt tttcttgttt ttatattcac
     3061 aatgttggct ttttattgca ttatcttgac agataaaaaa caaaaaataa tctaaaaaca
     3121 ttgatgcata agattgataa ctttttttac ataacaatat atacaaacaa aaaaattcac
     3181 taactagtga aaataagtta gtgaacctca tttattaaag aattaaatta cagatgatct
     3241 tttccatcat acttgaaaaa ctcaaaatca gcataacttc ctcttttttt atttagatca
     3301 tgcactgcta tcccgataaa atttccagta aaccctcctg agagtataca catgtttttt
     3361 tgcggaccta ctttcttcca ttcttcagtt gatacgtctt tcaaatgaaa ctgtgctgtc
     3421 tctttcttta cagtgacttt caaaaaagtc ttcttttctg tgagtggaat cgtccaatct
     3481 tctatttgtg ctactccgtt ttctgattct atcatacgta ggcattttcc cttgtcctca
     3541 ttccaagtaa cataacaata aatataattg gattcattta agtacagtac cagtccagcc
     3601 atctgattaa aattattagg catgtattca aatgctgtct ccgcatcaaa agtaaaatcc
     3661 gtttgccgaa tagctaacaa atgatgttcg aaggtagatt gaagagattc accggaaatc
     3721 agacgtaaaa aacctggtct acattttgta tcacaccaat cagcatttgg caaaatgcgt
     3781 ctggcattcc agttcttctt tagctctcct tcaaatgtat ccataaactg atgatctttt
     3841 tcttgttgta cctcttcatt tgtctcaatc attatttctt ccatcggagt tgtctggcta
     3901 tctttaagtc ttaaccaatc gtcggacgtc caatacactt cttgaatagc ggtttctctt
     3961 cccaaaatag ccgcaccgtt taatggtctg gtacaaagat atgtcatgta ccatcttcct
     4021 gacggggttt gtactaagct cccatgtcct gtacattgca atgtagaagc aggtttgtca
     4081 ctagctgtca gcataggaaa accaggatca agctcgtaag gaccagtaat attctttgct
     4141 cggcaaactg taactgaatg tcctcttcct gtaccgcctt ctgccgtcat caaataataa
     4201 tacccgttac gaaaatacaa atgtggtgcc tccgttttcg ctaattctgt acctgcaaat
     4261 attttataga tgggaccatc aagcttcttc ttctctgcat catattcttg taatacgata
     4321 ccgctggatt tattcggtgt gtccagacga taatcccata gttcattgac aagccatttt
     4381 tttcccgttt caagatcatg gaacaaagaa ggatcaaagc cgttactatt taaataaatt
     4441 ggttcggacc aaggacctgt gatctcagat gcagtgatca aataattgtg gacatcttta
     4501 aacggacggc ctgcactttt gacatctgtg taaacaagat aaaacaatcc atcagcataa
     4561 ctgatctgtg gcgcccatat acttccattt gtaggatttc cttgcaaaga tacttgagtg
     4621 gtcaaaatat ccgttgtatg tgaccagttg actaaatttt tcgactcata tactcggatt
     4681 cctggaagcc attcaaaaga ggagacaata atgtaatacc gctctttcac tcttaatata
     4741 ttaggatctg gattgaatcc tcttaaaata ggatttttca ctttgatagt ctgcatgatt
     4801 caggctcctt ttctcattct tactcgatat aaactgaatg ccccagctgc taaaaaagca
     4861 agtgaaataa acaacacaac ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta
     4921 ttttctaaag acaaagttga atgatctaca gcaagcgact gataactttt tttaaactct
     4981 gacgcagaca tattacttaa agtaactgct aacccgccaa agaaaatcaa agatagactg
     5041 ccaccgacca ttcctagcaa tagttcccat tttcttttca tttctgaacc ccctgctttt
     5101 ctaaatgtat ccattcaggt cttttgatcg atgtttcata attgttatta cgttcagaga
     5161 atttgacagt ctgttcattt ttcttgtcat cccaattatc ccaaccagtt gggtgaacat
     5221 gcccacctat cttacacgct tcaaataaga catttgcata cggacgccac ggtcttccta
     5281 aatagacgtt tctagtatca ggttctgcag tcaaggtaca gctttcaaaa cgataccctg
     5341 ttttttgatt tttcggcgta gatgctgctg tgatgtatcc aggattttta ctggctaagc
     5401 tatggatatg acactgatga aaaacggcat ctgccccacc aaatatgaaa tccaccgttc
     5461 cttcaatcca gcaatattca taggtctgac gacaatactc aggattggca gaagccggtg
     5521 tacaaaaagg agtaccatct ttttgcaaat caggcaacgg acccgtgcat aaggtatcct
     5581 gataaccaga tagtcgacag ttttttaaag atacgcgatc tcctgaattg aataaagcaa
     5641 cagcctgtcc taccttttct cctggacctg ctgtattttc gatgtgaaga ttttctaata
     5701 cgacattcga tccttctaag aaaaaagtag cacttcggaa agtccctaac tggctaccgt
     5761 caagatactc ctgttttgca aaacgattac cggagataac tactgttcct gctcccacaa
     5821 gtttaaaatt ggataatctg ctttctatat attcatcata tcttccttct tttactatca
     5881 agacctttgg atggctatcc ggcaggcctg ccaaataatc gattccttct tggatagttt
     5941 caaaatccgt cggggtatct atagcattct ttccaattat cagtttgtct ttcatcctca
     6001 taaatgctcc cttatgaatt gtttccaaat atttctcatt tcttgtgttt ccatatgacc
     6061 gcctgttaaa gaaaaagaag aaaagttttc tggactaccc attgattgat actttttgtc
     6121 cagccctttt ctcagcttca gtacgccttc gtccaagcac atcgtatcgt tttttccaac
     6181 taagctaaga cgagggcgcg gagcgatttt ttcttggatt gctaacgttg tataagctgt
     6241 caatagattt ggcacatagt aataaaaccc atgatgatct aggcggcgat gtagcaaaag
     6301 tgtttcgatg tccacctgtg ccgctagatc aatacaaact ttgacacgag aatccaatgc
     6361 tgtcacccac cagcttaata agcctcccat agaaagacca atcgttgcga gccgttgagt
     6421 atctgtgtct tcccttgtct ccaaataatc gatcaagctg atcacatcat aaatacgcat
     6481 tccccataac gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc
     6541 tccgcgttct tcaaatcccc aagcatcaat cgaccaaaca gcatatccca ttcctgtcag
     6601 tacttctgca aaagatggag aagctaagta aggagcacca tggatcaatt cacttttccc
     6661 gactgaaaaa tctccgccgt gagaatgcag gtaaaggacc gtcggaaacg gtccttcttt
     6721 ttctggataa gcatagaggg ctggtactgt ttccaattca tttaagtaca attgatagta
     6781 ttcaagcata aaaccgtttt gtttttctcg gtataatcgt tttccactag gggtatgtct
     6841 gctagatgga gcacctaaaa aatcattcaa ctgcataagc aaagttttta cgaacaacca
     6901 gttcatccat attttctgat tgatttgatt ggaatttctc aatactgatt tctttagtat
     6961 tttctaatag aaatgctggt ccctcgtgat tttcaatcga catctggtta aaggaaacct
     7021 tacccgcaaa gccaatataa aatccttgtc tgctcatttc ctctattcct gtcatcatag
     7081 caggaacacc gggaactgca tcttgggcca tcgagacatc gatattgtca aatgtgattt
     7141 cttgtatgaa ttgttccgct aatccataaa taaaacctgc agaagcatgg acattccttg
     7201 ccgtgatatt cgaaaaatgg atccttctga atgctgatgt ccgttcatct atcggataag
     7261 cctttttttc ccatacatac ggttctttcc ctcgcggacc acagaaataa taaagattca
     7321 gtataaatgg acacatcaca ttatccatga ctatattgga tactcgaata tcttcaacga
     7381 tcccgcctcg acctcgtcta gatttcaatc gtattcctcg atcagtttct tggaagatac
     7441 aatttgaaat ggtaatatta cgaatactac cactcatttc acttcctaat acgacaccgc
     7501 catgtccatg gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt
     7561 acgtatcttc agtccctgcc ttgattgcaa tacagtcatc tcccacgtca atatggcaat
     7621 tactaatacg cacatttttg catgattctg gatcaattcc gtctgtattt ggtgaatccg
     7681 ctgggttcaa gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc gggttgatcg
     7741 tccaacttgg tgactggatc aatttaatat ctttcacagt aattcgatga cagttatgga
     7801 aactcatcaa tttaggacga ggataagcga gattatctgg ttcatttcta aaagtatgcc
     7861 accatttttt tccatttcca tctaatgtac cgaaaccagt gacagagatg ttctctacat
     7921 tttgagcata gatacaggaa gcataaactt ttcgatggac tccttcccaa cgtgaatgaa
     7981 caacaggata atcttttgga tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa
     8041 gttcgacatt gctttttaaa aataaagctc ctgttagaaa ttcacctgct ggaacaacta
     8101 cggtcccgcc tccgtcactt gctgcagcat caatggcttg ttgtattgct tcagtattca
     8161 attcatcaat tgatgcgcca aatgtcaaaa tatcatacat atttataatg cctccacttc
     8221 aatcaatgct tgcaagaatg caccaacgcc tttttgatca ttacaaataa ttggttcact
     8281 gatgtagtaa gcatatgaac catctcgctg atctgctccg cctaaccctg ctacttgaca
     8341 gtttttattc aagttcagcc agccttcttt tgtttccaga acaaattcat caagtaatcc
     8401 ttgatgtgat ttcttcactt gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat
     8461 tcctttagcc attgcgtaaa caaacatact actgccggaa gcttccagat aatttccttt
     8521 ccgttttgtt tctgttgtta cttgatacca tgtcccgctg ttttcttctt gatatggaag
     8581 taatgcagcc agtgtctttt ctagaatttc tttcagcaaa gaacaatctg tttcttcatg
     8641 aagtaattca taggtatctg ctgcagccat caaataccag ccaatcgatc ggctccaaaa
     8701 atgatgggat agacctgttt ttggatcagc ccatggttgg accttctttt catcccatgc
     8761 atgaatcagc aaatttgttt ttggatcgaa tagatgttta taactgattt caaactgtct
     8821 caaaacatct tggtaatctt tttcttcact aaaattctgg ataaattcag cataaaaagg
     8881 agaacccata tataagccat ccaaccaaat ttgataagga taaatttctt tatgccaaaa
     8941 agctccttcg gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt ctgctgcttt
     9001 tttatatttt tcttcgcctg tttctttgta caataaaaag aaaagctttc ctgtatttac
     9061 atggtcgata ttgtattctt caacgctgta gccacggatc gttccatctt cttgcacgaa
     9121 atagtcgata ttgtctttta tgaaatcaaa ataaattttt tcttgggttc gttcccaaag
     9181 taattggaac cctttaagta cgacgccata gtcataagac cattttcctt tataccactt
     9241 ttcttcatat aagcgaggtg ttctttccat gatacttttt gctaatttct ttgaccagtt
     9301 ttccattatt tgctctcctt taaaaattaa aataggattt gggacaaata ccaagaagta
     9361 tttgtcccaa tcgtctatat tactgtgccg ctgctttgta tgcttcctcg tactcttgtg
     9421 tgattcgatc gcctccttgt gctttccagt cttctacagc ttttttgaat ccatcttcgt
     9481 caagctgtcc catgatgtat tgcaccgtag catcaatgac gattttttca atttctgatc
     9541 ctacttcatt gttcgtcggt gaatccaatg gcacagaagg atccattaca gcaaattctt
     9601 cattttcttt gatcaattga ttagagaatt ctttttcagg gtctgtatct ttgaaagaga
     9661 acgtcacttc actaggacga gagcttgata acggttgcac ctcttgttgc caaagatcca
     9721 tatcgataaa ttcgatagaa ccatcatcat tcaattcata atgcgttcct tcgataccgc
     9781 cagtcatcaa tttatacaca tcttcttcta aaagatcatc aacaaattga agtaatttct
     9841 tcaattgtgc ttcgtctttt acttgagatt tgggaaatgc tagcaagccg ccaataccat
     9901 ttccttctga ccaaacatga taaccgccgc cttcaccatc tgagatcttg tttacaggca
     9961 ctaattccat atcatcttga ataccttgac ccatcgtctt cagattcttg atatccacca
    10021 ttccagtgta gattccagtt cttccttgtg caaattgttc ttgctgatca gtcttttggg
    10081 taactgcaaa gtcttgtgcc aaatagccat tctcaaatag atctcttgaa aaattcaaag
    10141 ctttcatata ctcttctgta tcaaattctg gagtgaattt gccattatcg tctacagccc
    10201 agccatttgg tgctccaaaa taagaagtga aaagtttaaa gctggtataa cgaatatcac
    10261 taggcgcacg atcgccaaaa ccaactgtgt cgtccacacc atttccatca ggatcgtttt
    10321 ctgtaaattg tttagctact tcataaagct catccatggt agttggaaca tctagtccta
    10381 gattatccaa ccagtctttt cgaatgacta agccagcacg agcgtagtct ttttgtatcg
    10441 gtacgccgta caaggcaccg tcgatactcg cagcttcgat tctatcttca gaaatttgac
    10501 ttaaattttt atagtctttt aaatattctg atacatccca gaatacgcct gattttaacg
    10561 cattgcggac agaagagttt ttcaacatcg caatttgaag tgtaacgata tctcctagtt
    10621 catcagaagc caatgcagtt gttaatcttt cttcttttga agcatcagga atccagttga
    10681 atgtcagttc cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg
    10741 aagcatgatg caaaatattt aaccaagtga ttttagcttt tccatcttct gtctctgaag
    10801 aactgttact tgaactgctg ccgcatgcgg caagtgctag aattcctgca aaactaagac
    10861 ctaattttac gagcgttgct tttttcataa attttctcct tcactgtttt aataatattt
    10921 ttgttttctt ctgttttttc tgtatatgag ttcttccatt ccaaatggca aaatatagaa
    10981 tttgctgtcc acgtgactgc ggaaaagaag agactgccac cgataaaggt taaaacacct
    11041 ggcattgttt ggatgatcat cagataaatc cctaccatac ttaatatcaa cactaatgag
    11101 taatgaagat gtgagaatcc gaataaaaat gccattttca ttttgaaaaa atatcctttc
    11161 caatcataat gagccatgat tgggaaaata tagagtgctg agagcacata tatgaacgca
    11221 gcaatataca aagcgatcct aagataccaa ttgtgtatga aagaaagatc cacaagtaaa
    11281 acaatcccga tggcgccata tatccagctc atcagcaagg tttcacgata gttttcttta
    11341 aaatatgtga agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag ccattttcta
    11401 atcactgcaa ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg
    11461 actaaaccgc cccacagaaa attcaaataa accaactgta aaatacgatt tagccttccg
    11521 tttattttat ctaatgactg cagcatatct ttccctctct ttctatctta gtaaacgccg
    11581 tcttctccta gctttttggc aatcttatta gctaaaatta ccataaacaa tcctacgacc
    11641 cctttaaaca atcccattgc tgtactgaaa cttaactgtc cattcttcaa tccagctgta
    11701 taaatatagg tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa taacaagtac
    11761 atatgctcga accctaagtc taatgtctga ccgattttta gaatcagcaa ggtaatgatt
    11821 actggacgaa ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc
    11881 atttctgcag cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc
    11941 gtagaccagc ccaactcttt ccagatcact tgtccgatat atactgtgcg tgtccattca
    12001 ggggatgtta gaaaactgat tttttcaaat cccattgttg caaggaattc attcaatacc
    12061 cctccgtcaa cattcaaaaa tacataagta atcgaaacaa tgattaccca agacatgaag
    12121 tgagggatgt aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt tacttcattt
    12181 aatagtaaag acaaaatgat tggcatcgga aaaaacacga agatatttaa tccgaacaag
    12241 atcaacgtat ttcttaataa tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt
    12301 ttgaaaccga cccaaggact tcccattatt cctaaaaatg gttgataatc ttgaaaagca
    12361 ataacgagtc cccccatagg aagataccta aaaatcaaga aataaaggac tcccggcaga
    12421 atcatcacat ataaaaattt attcgttttg attcctgcaa ttattttttt tcgtcttctg
    12481 agcttttctt cttttttcga tagcgtcagt tgaaatggtt cttgtttctt tacactttcc
    12541 ataagcccct ccttagtttt gtgaatgttt gctgcttatg acttcatgat agataactat
    12601 ctgaattacg tatataatca tttttatcgt aagtaaaaac gcttacatag caatgatttt
    12661 tgtgctatac aaaacgataa agtgtatttt ttcacaaata aactacgcat agcaaacctt
    12721 ctattttttt ctatctgaca cttagcatag gaaatgatta tatacgtaat ctttcaccga
    12781 tgataaattg aacatataga aacagaaaca tttaatttat tttgaggagg agcctatcta
    12841 tatgaaaaag aaagtctcta taggagaaag aagttttaca cttttcaaca atacgtttct
    12901 aattttgcta gctttgatct gtatcgttcc ttttttaaat atcatcgcta catcttttgc
    12961 ttctacacaa gaagtcgtcg cgaaaaagtt tatccttttt cctactacct tttctttaga
    13021 cgcgtatcgc tacattcttt ctacgccgac catttttaga gcacttgctg tttcgattgg
    13081 tgttacaggt gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg cctacggtct
    13141 ttccagaaag tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt
    13201 gttcagcggg gggatgattc caacgttctt agttgttcgt tctctagggc tggtcaattc
    13261 ttactgggcc atgatcctgc ctgtggctgt taatgcaatg aatatgatca ttatgagaaa
    13321 ctttttccaa gccttgccgg atagtttgga agaatcagca aaaatggatg gttgcacaga
    13381 ttttggtgta tttttcaaaa ttatgctgcc attagcttta ccgtctattg ctactatctc
    13441 gcttttttat gctgttactt actggaacac ttatatgact gctattttgt atatcaacga
    13501 ttcatctaaa tggccgatac agattctttt acgccaaatc gtcattgtat caagtgggat
    13561 gcaagcagaa agtagtgccg ttgatgtgat tccaccagca caaacagtta aaatggctgt
    13621 tatcgttatt gcaacagtgc ctatgctgat tgtttatcct tttgttcaga aatattttgt
    13681 caaaggagca ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca aaatggtagt
    13741 tgtgtcagaa gtggtcagct tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa
    13801 tttttgtgaa atttcggctt atttttcgaa gaatctactc ctggaacacc gtgtatcaga
    13861 ggttgtgaaa gagacgttca gccttgagcg atgataaaaa ctctcacaaa cattgcgacg
    13921 agtaacccac aggagcaata agaagcgatt ttgaaaaata gcttcccata tttcacaaaa
    13981 ttatgcctta ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa aaagaactgt
    14041 gacaagactt ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa ctatccataa
    14101 atacaaatac tagggttatt ctaacttctt ttattgcttg aatcagtcca caagaaaaac
    14161 agcctcttga ttaaacggcg tttatcttgt tgattctgcg gcaataaaat cagccagcag
    14221 caaaacatga agtgctgttt tttgctgtcc tcttttattg cttgaatcag atcacaagaa
    14281 aaacagcctc ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt
    14341 ctttttaaag aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc
    14401 ttttacggag aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg
    14461 aacaaaatca ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg
    14521 gttataatgc aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta
    14581 gacgatatgc ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga
    14641 gcattctgtg attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg
    14701 ataattgttc agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac
    14761 tcccaataac tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg
    14821 actcaaagga tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt
    14881 cacttcctga tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata
    14941 tctaataagt gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact
    15001 tggaccagta tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct
    15061 ttcattcagc gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc
    15121 ttttacacgt ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc
    15181 atctttatcc attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat
    15241 tcgattttcc tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc
    15301 acgctcccca ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca
    15361 gtcattttga gaactataac caaattgctg caaccgctga tctagttctc gcctttctac
    15421 tcgatttcta aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa
    15481 gtgtgcactt aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc
    15541 tagttctttc ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat
    15601 cggacgggca aatctgtccg aaaataagta ggaaaaaaga ccaatgagtc ctaccagtgt
    15661 taaagacact gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga
    15721 gtcaacttct aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt
    15781 atctttttct cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt
    15841 tggttgctct ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat
    15901 agaaagtatg tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt
    15961 agcaattccc aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat
    16021 tcctttgttt ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata
    16081 cttttcaact tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc
    16141 agccaaactg actaaatcat aattattatc atccataaca atcgtttcta taaaatttag
    16201 ttctttcctg atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat
    16261 gtccgagaaa tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt
    16321 ccattcaacg ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga
    16381 ttcgatcgat tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc
    16441 tcctaataaa aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt
    16501 cctctcctcc ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat
    16561 taaatgagca ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt
    16621 ttaacgtgtg caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat
    16681 gatgaatgcg ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa
    16741 gatatcgcta aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc
    16801 agtttaataa aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat
    16861 tcgccaaact atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt
    16921 ttctcaagtt tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc
    16981 cattcagtat tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta
    17041 acagaaatca acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat
    17101 cagcctttca tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac
    17161 atcaatgatc cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca
    17221 gtagattacg gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat
    17281 tttcgttcga caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc
    17341 cctgtcaatc tagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac
    17401 atggaatacc tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac
    17461 atagcgattg gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt
    17521 tctctgatcg ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc
    17581 gtacgtaagc ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc
    17641 gaaggcagga cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag
    17701 tcgatcaatt acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac
    17761 acagaaagga agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac
    17821 ctgtaaaagt tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga
    17881 ttcagcagct gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag
    17941 aacaaggtct tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag
    18001 gactcagcaa tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga
    18061 tcaatcctta tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct
    18121 atatcgtctc caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag
    18181 atactccccc acagagcttc ccgggcaaac tgacagcctt tttataccga cgtttcaaat
    18241 taaacaaagc tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc
    18301 taaaagaaat cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt
    18361 ggttagaaaa agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc
    18421 ctcgcgaaga tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca
    18481 aagcagaacc attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac
    18541 cattcaaaca ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac
    18601 gtaaggtgca tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg
    18661 aattggaaac tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt
    18721 tattcaatct agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg
    18781 ctgaccaaat caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt
    18841 cattaaatag tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg
    18901 aagaacaaaa tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt
    18961 atcgaggcga tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg
    19021 cttggtctga taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag
    19081 aagatttgtc tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg
    19141 atcaaaaagg tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa
    19201 accctgcaac caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct
    19261 attccaaaac aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc
    19321 ccgcaaggtc ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat
    19381 ggctatccaa tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat
    19441 aatgtaaaaa ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca
    19501 agtcattacc tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta
    19561 gggattcgta atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa
    19621 ctgatcgtca aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg
    19681 attttaaagc atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa
    19741 attttagcgg acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt
    19801 tgtgagaaca atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga
    19861 gtgaaatttc tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta
    19921 gaagagcttt taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg
    19981 aatgtcggtt tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta
    20041 cttggtgcat tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg
    20101 ccagaaatgt ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt
    20161 gactcaatcg ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca
    20221 gtttacgaaa acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag
    20281 tctttaggct gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc
    20341 gaaaaaatca gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct
    20401 gcctctgctc ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact
    20461 ccgtttggct catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc
    20521 aaaggtcatt ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc
    20581 ttagaatcgt ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa
    20641 caaaatgacg ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa
    20701 aacagtataa aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag
    20761 acgattgggc gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc
    20821 actgtcactt agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag
    20881 cttggttagg aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag
    20941 aaaaaatcac aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac
    21001 caaaaattat cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg
    21061 gtattgactt gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa
    21121 ttttggcagc tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga
    21181 tttgtacaac agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag
    21241 aaccagattt gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag
    21301 aaggatttaa tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct
    21361 atcaagaact gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc
    21421 gtgtttccga ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag
    21481 aagagatttt tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt
    21541 atcttacaga aacactgata catttgaccc acttctatca ttctcataat tggacgatgc
    21601 agctgcacat ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg
    21661 ataccggcta tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga
    21721 accgtgcaga agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg
    21781 actatcctgc gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc
    21841 agttaggttc tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac
    21901 aatttgctga tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt
    21961 tcctgtccta cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa
    22021 tggtcatgcg aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga
    22081 ttagttatac aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca
    22141 gtaacgaatc aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa
    22201 gaagacggac caaattt
//
LOCUS       DACEMS010000039        21744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEMS010000039 DACEMS010000000
VERSION     DACEMS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.039"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            162..1901
                     /gene="yfcC"
                     /locus_tag="IW735_001315"
     CDS             162..1901
                     /gene="yfcC"
                     /locus_tag="IW735_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1044326.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2058..3242
                     /gene="iadA"
                     /locus_tag="IW735_001316"
     CDS             2058..3242
                     /gene="iadA"
                     /locus_tag="IW735_001316"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1044327.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3353..3556
                     /locus_tag="IW735_001317"
     CDS             3353..3556
                     /locus_tag="IW735_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1044328.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3657..4319)
                     /locus_tag="IW735_001318"
     CDS             complement(3657..4319)
                     /locus_tag="IW735_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044329.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4332..5834)
                     /locus_tag="IW735_001319"
     CDS             complement(4332..5834)
                     /locus_tag="IW735_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044330.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6382..8061
                     /locus_tag="IW735_001320"
     CDS             6382..8061
                     /locus_tag="IW735_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1044331.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8188..8667
                     /locus_tag="IW735_001321"
     CDS             8188..8667
                     /locus_tag="IW735_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1044332.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8886..9566
                     /locus_tag="IW735_001322"
     CDS             8886..9566
                     /locus_tag="IW735_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1044333.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9574..10905
                     /locus_tag="IW735_001323"
     CDS             9574..10905
                     /locus_tag="IW735_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1044334.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10920..11882
                     /locus_tag="IW735_001324"
     CDS             10920..11882
                     /locus_tag="IW735_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1044335.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11993..12331
                     /locus_tag="IW735_001325"
     CDS             11993..12331
                     /locus_tag="IW735_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1044336.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12410..13117
                     /locus_tag="IW735_001326"
     CDS             12410..13117
                     /locus_tag="IW735_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1044337.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13220..14701)
                     /locus_tag="IW735_001327"
     CDS             complement(13220..14701)
                     /locus_tag="IW735_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1044338.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15010..15546
                     /locus_tag="IW735_001328"
     CDS             15010..15546
                     /locus_tag="IW735_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1044339.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15624..16994
                     /locus_tag="IW735_001329"
     CDS             15624..16994
                     /locus_tag="IW735_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1044340.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17061..17396
                     /locus_tag="IW735_001330"
     CDS             17061..17396
                     /locus_tag="IW735_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044341.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17477..18334)
                     /locus_tag="IW735_001331"
     CDS             complement(17477..18334)
                     /locus_tag="IW735_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1044342.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18441..18956
                     /gene="tadA"
                     /locus_tag="IW735_001332"
     CDS             18441..18956
                     /gene="tadA"
                     /locus_tag="IW735_001332"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1044343.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18979..19063
                     /gene="ffs"
                     /locus_tag="IW735_001333"
     ncRNA           18979..19063
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW735_001333"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19273..20658
                     /locus_tag="IW735_001334"
     CDS             19273..20658
                     /locus_tag="IW735_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1044344.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20695..21372
                     /locus_tag="IW735_001335"
     CDS             20695..21372
                     /locus_tag="IW735_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1044345.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21380..>21744
                     /locus_tag="IW735_001336"
     CDS             21380..>21744
                     /locus_tag="IW735_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1044346.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLD"
BASE COUNT         7114 a         3634 c         4664 g         6332 t
ORIGIN      
        1 agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag tgattttcta
       61 ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt atccattttt
      121 tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa acaaattctt
      181 caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat gtcattattt
      241 ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga aagttcagca
      301 cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact gttttacgtc
      361 aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat caattagaag
      421 aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa
      481 aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa atctccttga
      541 cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct aaaaaattgc
      601 ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc ttaaattttg
      661 tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc gctgcattga
      721 tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc gacgcaggta
      781 tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg ctattattct
      841 ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt ccattctcaa
      901 tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca gtaaccgtga
      961 cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt agcgtagcgg
     1021 tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga ttgattatgt
     1081 gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa aaaatccgta
     1141 aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct catatcaaaa
     1201 agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta ttagagatgt
     1261 tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac attccagaaa
     1321 tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt cttttcaaat
     1381 tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca gctgatttag
     1441 ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg ggaggatctg
     1501 atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg
     1561 gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg
     1621 ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct ccactggcag
     1681 atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt tcaggtttta
     1741 tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt gcacggatcg
     1801 aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt gttttaggaa
     1861 ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga cctgtaaata
     1921 aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg gtaaaatcaa
     1981 aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag ttatacttaa
     2041 agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca ccagattact
     2101 taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa gaacagctag
     2161 aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa gtactaaccc
     2221 cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc ggttatcaaa
     2281 atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg acagttgtcg
     2341 gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc aaagcgaaag
     2401 gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga ttgccggtaa
     2461 aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt atcggaatcg
     2521 gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag ttcgctcgtg
     2581 cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc atcaatgtcc
     2641 acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa gaaacagaaa
     2701 ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta tttgaagctt
     2761 gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa gatcctgatt
     2821 tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga ttgatcgagg
     2881 aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc ttgccttatt
     2941 ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca ttgctagtag
     3001 ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta cgagctataa
     3061 ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg
     3121 atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt atcgctaaag
     3181 gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa aaaagttttt
     3241 aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg
     3301 ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca caatggcaga
     3361 tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa aagaagcata
     3421 cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat cactaggtgc
     3481 tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa
     3541 agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa aaaatccgaa
     3601 ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc tgttttttaa
     3661 acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca gctttttgct
     3721 acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc gatttcttgg
     3781 aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg ctcatccgct
     3841 acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg ctgctgacct
     3901 cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac acgagccagt
     3961 cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc cattgctgct
     4021 atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa aataatcgag
     4081 acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc ttgatagtat
     4141 acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt cgtttttcca
     4201 gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag ttctacatct
     4261 ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt ccgtaacatc
     4321 aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat ccctatagaa
     4381 gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag tcccaattgt
     4441 gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa tggattattc
     4501 gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc tggcatttgt
     4561 ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc aagtagctgc
     4621 tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa tgcaagaatc
     4681 atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga ttctcctaaa
     4741 gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat gatcagagta
     4801 agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa acttgccacg
     4861 ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa gctaaatgta
     4921 tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc tggatcagat
     4981 agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag ttcatttgca
     5041 aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt catacccata
     5101 ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata agtggtatct
     5161 tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt tgtttctgct
     5221 agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat tccttcacca
     5281 tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc tgacacacca
     5341 ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc agcaggttca
     5401 ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt gatggcagtt
     5461 attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt tacattatct
     5521 aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa actgcctgga
     5581 tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt tttaaatgaa
     5641 gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc ttgattcgct
     5701 gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag aatggctgtg
     5761 aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt ccatgcacgt
     5821 ttcataaaat tcatattttg catccctcct agtagatgca tttgataata gacaacctat
     5881 cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt tctgtatata
     5941 taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac
     6001 cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac agagggattt
     6061 ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat ttgttgtata
     6121 ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt cgcaaaagat
     6181 ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca gaacgcggat
     6241 gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt tgcatatgct
     6301 tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta aacttggatg
     6361 tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg cggcgttcgt
     6421 gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat tttagattgt
     6481 gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc tgattttacg
     6541 tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg acatgcagat
     6601 gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt cgggacagag
     6661 ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa aggatttaag
     6721 gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt agtgagcttt
     6781 ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac aaaagaaggt
     6841 agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca
     6901 gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact aagtgaatcg
     6961 gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga tgaagtattt
     7021 gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa
     7081 cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt cttgactggt
     7141 caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca attaccaagt
     7201 gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga actgatcatt
     7261 ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc aaacggtaca
     7321 caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac gccaagcatc
     7381 gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg tggaacagtg
     7441 aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga cttgcaatta
     7501 atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta tcgtgaatta
     7561 gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat ctatattact
     7621 ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg aactacaagt
     7681 gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat tgttttacgt
     7741 gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat cagtcggcga
     7801 gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta cgtaaaagct
     7861 agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga gaaacattta
     7921 gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca attaggccgt
     7981 tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat ggaagctact
     8041 caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata gagtgctcgt
     8101 cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc gctttataat
     8161 aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga acgttattta
     8221 gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga agaatctttc
     8281 acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac tctcgtagtg
     8341 aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca cttagattac
     8401 aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat ggattttgta
     8461 ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa actcgcacat
     8521 cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt aattgtgtca
     8581 agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa gatcgtccat
     8641 cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca gagcagagac
     8701 ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc tgttacaatt
     8761 gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta tattttttca
     8821 ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt
     8881 aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg gaaaatggcc
     8941 aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta actgcagaag
     9001 aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata ggagatatga
     9061 ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt gctgattatg
     9121 cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat gcgtttccat
     9181 tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta gacggtatcg
     9241 ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa acgatttatc
     9301 atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa ttcgatattg
     9361 atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat gcagcaaagg
     9421 ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact ttccctgtca
     9481 ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga aaaataatga
     9541 gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga tttgatttta
     9601 gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat cgcaaaagaa
     9661 ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta
     9721 gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa aaaacatatt
     9781 gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt aggcggggcg
     9841 gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa acatgccatg
     9901 gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga agtccaaaaa
     9961 ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac gactgctgag
    10021 attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta tgatacattt
    10081 acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg
    10141 cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca gacagatacg
    10201 atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac
    10261 cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa atcgccaaac
    10321 cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt
    10381 gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt cgtttccgat
    10441 gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat gcagcttgtc
    10501 cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa tatccatacg
    10561 attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt cgtagtagca
    10621 ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga aatcatttct
    10681 gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg
    10741 aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg tccacgtgta
    10801 ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga aaacaccatc
    10861 gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa
    10921 tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc attatccaca
    10981 ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc gaactattac
    11041 gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa gggcttcgct
    11101 ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta gtcatcgtga
    11161 ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca gaaaaactga
    11221 tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa
    11281 aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct gaatctttaa
    11341 agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat acagggcgct
    11401 ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg agaaattatc
    11461 catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac
    11521 tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga agaataatcc
    11581 ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct tcgatcgttt
    11641 cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag cagccttcag
    11701 ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg gctaaacgcc
    11761 atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag
    11821 aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga aaaacagaat
    11881 gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc ttttcaagat
    11941 cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga agatgacatt
    12001 accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct tgttgatttg
    12061 cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag aagacggatt
    12121 agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga cagtgattaa
    12181 agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat
    12241 ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg gaccgatgaa
    12301 actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt cacattcttt
    12361 tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta
    12421 tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat tagttatttc
    12481 cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa
    12541 aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac agataaataa
    12601 tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag gtaataagat
    12661 gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc
    12721 tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga agattcgtgc
    12781 agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga ttatggtagg
    12841 tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt tttctttagc
    12901 attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc gagcaatcca
    12961 aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa gacacgtatt
    13021 atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc agctgttgcg
    13081 cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg tgaaatcagc
    13141 gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt tggaataaga
    13201 ggatgtgaca agaattttgt cacatcctct ttgttttgtt ctcataacct tttttcatat
    13261 acagttcatg aaccgctttt attaacttgc caaataacac tgtattgtgc gttgtatcca
    13321 gatagaaagt ctccgtttca tctgaaattt tctcatgctg atctgtaatg atcaaatcag
    13381 catgttctga atatgcggta attttaacag catctttgcg aaataactgc tgcaaatggc
    13441 tttgaagatg ataatttcct gtaaactgtt tggttatctg caaatggatg agtaactgct
    13501 ctttcgtttc tgtttgtagt aagtgttgga tcaattgact aaggtattga ctgatttggt
    13561 ttctttcccc attttttgaa aaaattgtat cgattttttc tcttaatttc ttttcctgta
    13621 taaaagaact agaatgattg gacgtggctt ctggaaagca aagtgtagaa aaactactgc
    13681 tggattcacc caacagttcg aaaaaaacaa ctgccaacac aagaaaataa aaataaattg
    13741 gtattttatt ttctaaatgg ttgagaggaa actgttgagt taagtaagtc atcaattgac
    13801 cgacttgtcg acaaataagg tttttgcttg tggaaaataa aaaaccaatc tcctgttttt
    13861 gtcgaaaaga gatttgattt ggaaaaaaac aatagaaaaa ataattaaag taggctctct
    13921 ctgtttctgt ctcttcacaa gggcgactca ctgaaaaaga agtaaagtat tctgaataat
    13981 catgtgtatg ctgcaataag tccatcaatt cagtgatttc agaagaagtt tttggaaaat
    14041 atgcctgttg tttgtttcgt aatatgaata aagttaacag caagtagata tttttttctt
    14101 gttttgaaat tttttctatt tgctcatctg tactgttcat ttttagtgca tttaattcaa
    14161 gtgaggaaaa tggccaatcg ttacctccaa atgtatctga aagaaaaaag taaagataga
    14221 gacggataga taactcattt ccttcaagag tacagcaatt ttttacagat aggatccgta
    14281 aatggaattt ttttaaggaa tgattgagct gttttgtcag tttttcgaga taggagggcg
    14341 atatataaag ttgttcaagt aaatattttt ttggtacagc agtttgtgga taattgatta
    14401 gtaacatcaa atagttaaaa cgattggatt cttttaggta gcataatttt aacttagaaa
    14461 aaacagctaa ctttttagtt tgatcggttt ctttggtttg gatctctcct ttatgattag
    14521 aataaataaa ttcatcaatc aataaaaatt gcttgagtga tacatttatt tttcggatca
    14581 gttctttgat tgtccgtttt ggaatatgta agtcattgga taattcttgt aaagaacaat
    14641 tatttttttg aatgaccatt tgtagaacaa gtaactcttt ttttcttgct tcaattagca
    14701 taaatacctc ctttttattc tgtttttgta tatagttaga caaaaactgt gagtggaaag
    14761 tcatttcagt ctgtgttatt ctcacagagt gtcgccttct tttttatata acgttattta
    14821 atttttttgt taccttgttt attctattgt ttatttccta gggtattttg tcaagatatt
    14881 cttgttttta aggcggaaaa attgattttt aatggatata actaaatatg ttttgataat
    14941 tatcaataat atttttagaa ttatttatgt tatttattta tgggtagatg aaaagagacg
    15001 gaggtaagaa tgatggggag agtaacaaat tatagtaaag aatatttgct gttcaaaagt
    15061 atggtatacg tggaagaata tggaatgaag tcaattactg caagagagtt agcagagttc
    15121 tgcggttgtt ctacataccc gatttataca catttcaaat caatcagcgg gttaaaagag
    15181 aagatattag aagaaatcac tgtttatttt gaaaactact tagctgaatg cagttccgat
    15241 gatgttttaa gcattgtcta cttattgaaa gatttttttt ctatccatga aagtattcgt
    15301 gaaatcattg caaaatctga tttagacatg gattcgctat ttaagcggcc atttttctca
    15361 tatgtcaaag gtcacacgaa gatcaaagaa gaaaagtcgc ttgaattggt atggcttaat
    15421 gcattgggaa gtttgcgctc agatgattct tctcaagatt ttcttgaaat gatgaacgct
    15481 gtttgggata aaaacgaggt gacggatgaa tacttggaaa atatcttaaa ggaacagtca
    15541 ccttaggcag caacatgaaa aagtagatca gccaccttta tttttcgtaa aaaagattgt
    15601 aaaatgtcta acgaaacatt ggaatgaagg aggaagcgac aacgttacca cagcaattta
    15661 tcaaaaaata tcgtcttctt ttaggcgaag aagcatccga ttttttttct gcgcttgaac
    15721 aaggaagcgt gaaaaaagga tttcggtgga atccactaaa accggcagga ttggacatgg
    15781 tccaaactta ccatagcgaa gaattacaac ccgctcccta cagcaatgag ggatttcttg
    15841 gcactgtcaa tggaaagtct ttcctccatc aagctgggta tgaatatagc caagaaccaa
    15901 gtgcaatgat cgttggaaca gctgctgcag caaaacccgg tgaaaaagtt ttggatttgt
    15961 gtgcggctcc cggtggtaaa tcaacccaat tagctgctca aatgaagggg aaagggttat
    16021 tagtgacgaa tgagatcttt ccgaaacgtg cgaagatttt atcagaaaat atagaacgat
    16081 ggggagtttc gaacgcaata gtgacaaatc atgcacccgc agaacttgtc cctcattttt
    16141 cgggcttttt tgatcggatc gtggttgatg ctccttgttc aggagaagga atgttccgaa
    16201 aagatccaaa tgcaataaaa gagtggacag aagaatctcc gctatactgt caaaaacgtc
    16261 agcaagagat tttgtcttca gcaataaaaa tgctaaaaaa taaaggacaa ctgatttatt
    16321 cgacttgtac gtttgcacca gaagaaaatg aagagatcat tagctggctt gtagaaaatt
    16381 atccagtaac gattgaggag attcctttga ctcaatccgt tagttcagga cgttctgaat
    16441 ggggaagcgt agctggtttg gaaaaaacaa tacgtatatg gcctcataaa gatcagggtg
    16501 aaggacattt tgttgcaaag ctgacttttc atggacaaaa tcagatgcat aaagaaaaga
    16561 agacacgaaa aaaaagtaaa gtgcaaatga caaaagagca ggaaaaactc tggacggagt
    16621 tttcaaatga ttttcattat gaagctactg gaagactttt agtgttcaac gaccatcttt
    16681 gggaagttcc tgagttagcc ccttctctcg atggtttaaa agtcgtacgt acaggtttgc
    16741 atttaggaga tttcaaaaag aatcgatttg aacctagcta tgcattagcg cttgctacaa
    16801 aaaagataga gaatatacct tgcttgccga tcactcaaaa ggaatggcaa tcttacacag
    16861 caggagaaac atttcaacgt gatgggaatc aaggatgggt tttgctagta ttagataaga
    16921 tacctgtcgg atttgggaaa caagtaaaag gaacagtaaa gaactttttc cctaaaggat
    16981 tgagatttca ctgagtggat tagttcgttt atttttttgt ctatgctatg atggagatag
    17041 ataaaaagga gctgatactt atgaaaacat caacaaaagt aacaatcgga ttaagcgtag
    17101 cagccgctgc cagtgtagcg acagcagtcg ttgtatcggg aaaagtaatg gaaaagatcc
    17161 atcatatgac tactcggacg aaagtaaaaa aattcgtcta tgacaaattt gatggaaatg
    17221 aaaaattact agaaatcgta gatggtcttt cagatagtga cttggattct ttgatgggaa
    17281 tgttagcaaa aatcaaatca ggaaaaaaga aaatctctgt ttatggtgat tcaatcaaag
    17341 attcaaccga agatgtaaaa gaccggctaa tgcattttgt tgaaaaaatg atgtaagaga
    17401 aactagcttt tgaacactat tcattcgaaa taaggggttg tgacaaaatt attgtcacaa
    17461 cccctttaat ctatctttaa ttatgttcat gaagcaataa aagatagcaa aacagtaaat
    17521 cgttggcttt ttttagattg aaaccagact gttcttgaaa tttttcgatt cgatatttta
    17581 gagtattacg atgaagaaac agtgtttttg cagcagagct gacattgcct aattcattcc
    17641 ataattcttg aatgatcact tccatttcat tttgtttgat cagttctatg taacttttga
    17701 tcaacttact atcttttaca gtatcttttg tatagtaatg taaagccaca tcttgcatag
    17761 aaaaagtacg gctgtgagta taaagattgt tttgctctgt taaaaaaatt tttctttctt
    17821 cagcaaacaa agggacgagg tcggtgccag gtgaatggaa agaaccaacg aatacagacg
    17881 ttgtcgtatc aaaatcagca tctaaagata gaaaaatacc gttgatttca gtagcatcaa
    17941 gataatcacg gtttatttgt tcgactaaga gcgcatctgt gtctgtataa aaaaagaaat
    18001 cagcagaata gcgaaataat tccaaaatag tatcttgcca ttcttttttt aagaattctc
    18061 cttttgattc gacgtgaagc tggatcaaac gaaagctttt ttcttgaaca caagaagcat
    18121 ctttaaaaag atagtgatac catgggtgtt ttttttgatc gttgattaca gggaataacg
    18181 ctttcaaaag gctggtttct tgttgagata atgaatcttg tttgatccag ataaaagcat
    18241 catcaattgc aaaagaaagg atatccgctt ctgatgctgg agagtttttt ttctgtgctt
    18301 gtgggtaaag agcaagtaat ttatccattt tcatcttgtt cacctcttgt atttatttta
    18361 ccatagaaaa aaaagttagt tacagccttt ttatcatatg ctaaagtaaa acaagaaaaa
    18421 acaaaggcag ggaagggtaa ttggtaaata caaatcttga tgcagtagaa aaagaaaaat
    18481 ggatgcgcct tgcattggca gaagcaaaga aagcagaaat gcttcatgaa gttccgattg
    18541 gagcagtggt tgtattggat ggaaaagtga ttggaagagg atataacctt agagaaacca
    18601 cgcaagatgc tactacacat gcagaaatgc ttgcaatcaa ggaagcctgt gaaaaagtgg
    18661 gaagctggcg cttggaagat gctgcacttt ttgttacctt agagccttgt cctatgtgca
    18721 gcggcgcgat gattttgtct cgtgtagcag aagtttattt cggcgcgtat gatccaaaag
    18781 gcggaacagc cggtaccttg atgaatcttt tggaggacga gcggtttaac caccaagcat
    18841 atgtagaagg tggcatttta gaagacgaat gtgggatgat tttgactgac tttttccgaa
    18901 agctccgcca gcgaaaaaag aaattaaaaa aagactggtc aacgaatgaa aaataaggta
    18961 tactattctt tgccgaaagg ctaggacaat ggtgggcttg tgaagcgtgt caggtctgga
    19021 aggaagcagc actaagcagg ttccgccatg tgtctgatta taatgaaacg acaacagact
    19081 ttcgggtctg ttttttttat ttttatagta ctgtaaaaat gaatgttcct gattttttag
    19141 ccagactgat ttatcgaata ttgtacttat acaatttgct tcaaggcagg aaaaactctg
    19201 aaatagagct tgtttcaatg gaagaaaaag ctgcctatgg tatgataaag ataaagagag
    19261 gcgagagggt acatggaaaa aatagtgatt ttacatacga atgatctgca ttctcattta
    19321 gaaaattggc caaggatcag aaggtttttg gatcaacgta aacgagaaaa cgaaaagaaa
    19381 gaaaaaacga caacgattgc agtagatcta ggtgattttg tggatcgttg gcatccattg
    19441 tcagaagcaa cgaatggaca ggaaaatatc ttgctgatga atgaagttgg ttatgatgca
    19501 gcgacgatag gaaacaatga aggcgtagga aattccaaag aagagttgaa tcatttatac
    19561 gatcatgctg agtttgatat catcctggat aacttattcg ataaaaatac gctccagcca
    19621 ccggcatgga cgaaaccata taaaatcatt acaacgaaac accagacgaa aatcggacta
    19681 attgcattta ctgctccatt tccgcttact tataatccga atggctggga catacggaat
    19741 ccttatgata ttttgccaga attagtcgaa gatttacgtt cacaagtgga tgttttagtt
    19801 ttgatgagtc atctcgggat tcaggatgat ttccaaattg caaaagaaat accagcaatt
    19861 gatgttattt taggatcgca cacccatcat ttattcagag aaggtcacgt aatcaatgat
    19921 gtccaaatag cagccgctgg aaagtacggg cagtatgtcg gtgaagtcca tttaaccatc
    19981 gatgaagaga aaaaaatcct taagcattca gccaaagcca tccctaccga aacaatgaca
    20041 gcttttacag aagatgaaca agaaataaat agttatctag aacgtgggca tgaattatta
    20101 aaagaaaaag aagtagcaga tttgccattt tctctgagtc tagatatctt caatgagcat
    20161 tcgtttatcc aagtagcctt tgaggcggtg aaagaacgag gaaagacgga agcagcaata
    20221 ttaaacagtg gtctgttttt aactgatatt ccaaaagggc gggtcaatca ggatcagctt
    20281 cacactgcgt tgccgcatcc gatgcacttg ttaaatgtca cattgggtgg aaatgatctg
    20341 atccgcttag ttttagaaat agaaaaaaat cgtaactttt tacgtaacta tcctatgaag
    20401 ggaatgggat ttcgcggtaa gatttttggg cagatggtct ataatggaat cacatatgat
    20461 tctattaacc atcaagtctt ttggaagaac aagccaatcg atctgaataa aaattatact
    20521 tttactaccg tagatcattt gatgtttgtt ccattttttc caacgattga gattgctgga
    20581 aaaaatgaat ttttgttccc tgaatttatc cgaagtgtgg taggagatta cttgaaagct
    20641 cactacccaa tcaaataaaa acaaggtata ataaaaaaga taggtggtga acgcatgtct
    20701 gcaaaacaaa atgaacagca gaacgttgag cataaaacag aaattgctaa gaaaaaatca
    20761 tcagctgaac aggaaacaac agtcaccgaa gaactgacag ctgctttgga agaaatcgtt
    20821 gaggagccta aagcagaaaa agtcaaaggc gagctagtga cactgttaga tgacagtaat
    20881 tttttgatag gtgaaagaca ttatcggctt gtgtccgatt atcgtgaagg atttaatgca
    20941 gaaaagcttg gagaacgtta cagtgatgta ctagcccgat atgattatat cgtaggagat
    21001 tggggttatg aacaactacg cctaaaagga tttttccaag cggataatcg acgggcgcat
    21061 ccagatcagc gaatcgattc cttggaagac tatctttacg aatattgcaa ttttggttgt
    21121 gcgtattttg tcatcgagag aatcggcggt aaaaaagaaa agacaactca gcgccgaaaa
    21181 aagaaaaaaa accataatcg tgcgtatatc gatgagaaaa aaggaccagt cgcatctaat
    21241 cgcaaaaaac cggttattaa aaatcgaaaa acagagtcag caaaaacctc tgaaaaagta
    21301 aaaaaacaag aacaacctaa acaagtagaa aaaacaaaaa cagcatttac gatcagaaaa
    21361 agggaagagt agtggatgaa tggactataa aggatattta attgatttag acggaacgat
    21421 ttatcgtggg acacaaccca taccggccgg taaacgattt gtggaagaat tgcaaaaaag
    21481 aaaattacct ttcttgtttg ttaccaataa cacgactaag tcaccagaaa cagtagctaa
    21541 tcgtctagca gatgagtttg acattcatgt agctccagag acagtctaca cagctacttt
    21601 ggcaacaatc gattttatga aagcagatgg aaaagggaaa aaagtctatg ttatcggaga
    21661 agcaggattg attgacttga ttttagctgc cggatttaca tgggaagaag aggctcctga
    21721 ctatgtagtg gtaggcttag ataa
//
LOCUS       DACEMS010000040        21516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEMS010000040 DACEMS010000000
VERSION     DACEMS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.040"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="IW735_001337"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="IW735_001337"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1044347.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="IW735_001338"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="IW735_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1044348.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="IW735_001339"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="IW735_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1044349.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="hflB"
                     /locus_tag="IW735_001340"
     CDS             complement(3986..6097)
                     /gene="hflB"
                     /locus_tag="IW735_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1044350.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="IW735_001341"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="IW735_001341"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1044351.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="IW735_001342"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="IW735_001342"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1044352.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="IW735_001343"
     CDS             complement(8297..8767)
                     /locus_tag="IW735_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1044353.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="IW735_001344"
     CDS             complement(8813..9238)
                     /locus_tag="IW735_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1044354.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="IW735_001345"
     CDS             complement(9305..9574)
                     /locus_tag="IW735_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1044355.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="IW735_001346"
     CDS             complement(9585..11180)
                     /locus_tag="IW735_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1044356.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="IW735_001347"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="IW735_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1044357.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /locus_tag="IW735_001348"
     CDS             complement(14733..15293)
                     /locus_tag="IW735_001348"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1044358.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="IW735_001349"
     CDS             15646..16620
                     /locus_tag="IW735_001349"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1044359.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="IW735_001350"
     CDS             16790..18190
                     /locus_tag="IW735_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1044360.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="IW735_001351"
     CDS             complement(18268..18990)
                     /locus_tag="IW735_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1044361.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="IW735_001352"
     CDS             complement(19069..19575)
                     /locus_tag="IW735_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1044362.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="IW735_001353"
     CDS             19698..21497
                     /locus_tag="IW735_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1044363.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5973 a         4703 c         3864 g         6976 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttctcctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 ggatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta tacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa ataaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggtcga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctgggaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ctgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaata
//
LOCUS       DACEMS010000041        20818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEMS010000041 DACEMS010000000
VERSION     DACEMS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.041"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(39..1241)
                     /locus_tag="IW735_001354"
     CDS             complement(39..1241)
                     /locus_tag="IW735_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1044364.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1358..1873
                     /locus_tag="IW735_001355"
     CDS             1358..1873
                     /locus_tag="IW735_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1044365.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2072..2518)
                     /locus_tag="IW735_001356"
     CDS             complement(2072..2518)
                     /locus_tag="IW735_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1044366.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2565..4043)
                     /gene="msr"
                     /locus_tag="IW735_001357"
     CDS             complement(2565..4043)
                     /gene="msr"
                     /locus_tag="IW735_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1044367.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKVQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4397..5026)
                     /locus_tag="IW735_001358"
     CDS             complement(4397..5026)
                     /locus_tag="IW735_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1044368.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5010..5474)
                     /locus_tag="IW735_001359"
     CDS             complement(5010..5474)
                     /locus_tag="IW735_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1044369.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5961..7229)
                     /locus_tag="IW735_001360"
     CDS             complement(5961..7229)
                     /locus_tag="IW735_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1044370.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7460..8197)
                     /locus_tag="IW735_001361"
     CDS             complement(7460..8197)
                     /locus_tag="IW735_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1044371.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8264..8833)
                     /locus_tag="IW735_001362"
     CDS             complement(8264..8833)
                     /locus_tag="IW735_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1044372.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8961..9833
                     /locus_tag="IW735_001363"
     CDS             8961..9833
                     /locus_tag="IW735_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1044373.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10175..11047
                     /locus_tag="IW735_001364"
     CDS             10175..11047
                     /locus_tag="IW735_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1044374.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11044..13002
                     /locus_tag="IW735_001365"
     CDS             11044..13002
                     /locus_tag="IW735_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1044375.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13253..14104)
                     /locus_tag="IW735_001366"
     CDS             complement(13253..14104)
                     /locus_tag="IW735_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1044376.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14227..14694
                     /locus_tag="IW735_001367"
     CDS             14227..14694
                     /locus_tag="IW735_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1044377.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14957..15439)
                     /locus_tag="IW735_001368"
     CDS             complement(14957..15439)
                     /locus_tag="IW735_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1044378.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15539..16402)
                     /locus_tag="IW735_001369"
     CDS             complement(15539..16402)
                     /locus_tag="IW735_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1044379.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            17000..17848
                     /locus_tag="IW735_001370"
     CDS             17000..17848
                     /locus_tag="IW735_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044380.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(18019..18360)
                     /locus_tag="IW735_001371"
     CDS             complement(18019..18360)
                     /locus_tag="IW735_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1044381.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18446..19153
                     /locus_tag="IW735_001372"
     CDS             18446..19153
                     /locus_tag="IW735_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1044382.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19140..19463
                     /locus_tag="IW735_001373"
     CDS             19140..19463
                     /locus_tag="IW735_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1044383.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(19552..20589)
                     /locus_tag="IW735_001374"
     CDS             complement(19552..20589)
                     /locus_tag="IW735_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1044384.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         6202 a         4309 c         3695 g         6612 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt tttcaaaccg atccgactag ataagaaacc tgtaaatagc
     1021 atcaccacac cagtgactaa catgtaacta gtaaataaca gggtcgtttc agctggcgta
     1081 gcgtttaact gtgcagcaat cgtttttaag attgggtcta ctagacccac tcccataaaa
     1141 gcgatcatac aggtagctgc tacaactagc gctgaacttt tcccctttgt ttcttcattt
     1201 gtttgttctt gtatatctat ttgattctcc aatatttcca tccctttctc tctattagca
     1261 tcatacatga tttattttac atgtaaatat gcacatgtaa aacgtggaaa ctttactgtg
     1321 atataataaa gaaaataaga cgagaggtag gatttttatg aatactttag cctatatatt
     1381 attatgcgtg cttttatcca aaccacgttc tgggtatgaa ttgaagcagc tgatcacaat
     1441 tttttgggaa gcccatcaca gtcagatcta tacaacgctt gccaagttag accagcaggg
     1501 ttatgtgacc gttttggaca aagacgagca ctctcaaaaa aagatctacc acttgactga
     1561 tgaaggacgt gctcttgtag aggaatggat caaagaggaa actcccgctc ctacgcaaaa
     1621 agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc gataaaacaa cagctactgg
     1681 actgctattc actagagaac aacagctgaa caaaatcatg caaaaaaatc aaaaaaaatt
     1741 gaatgacctc tcgctagaca agaaacaaga atttggacgt tatgttgtga tccagcgcag
     1801 aattatgctt tgtcagcagg aactccaatg gtgtcagcaa gtaagagaac aaatgcagca
     1861 gttttttgag taagggataa acagaacctt tctagaaata tctattgaag agcaaattat
     1921 cttcaataaa agagcaatac agttggaaaa gaaaaagcga tccgaacgac aggccaaaac
     1981 ctatcgttcg gatcactatt tttctagaac tgcattcaag agactatgac aaaaagcatg
     2041 ccaaggcctt ttttcaaata acagtgttcc atcattcatt actttgaatt gcttttagca
     2101 cacttcttga aagcgggcca acgatgatca actgcaacgg taaagcggca atcacattca
     2161 ttttccaagc attcatataa gcagagaata attgatcagg tattccttgt tccatgatca
     2221 caccgaataa agacatacat gtcaccattc ctaaaaccat caagcatgaa atagttaaga
     2281 tcatttggac cttgctttct ggatttactg gtagtttaaa ggcaattttt ttcgccagca
     2341 caccgatgat gaagacatcc aaaatgaaag ccaccacgaa tccaggcact agaccaatca
     2401 acaaacttga taaatgaaga tcttgatgca gccataaatt atacgcgctc atccccaaga
     2461 ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc ttttttattt gtaggcaatc
     2521 tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc acatttaaaa atttctcgta
     2581 agtacttttt ttattttctg cccttttatt tcataaactt catcagccgt acgctcgaca
     2641 aagtaagaat catgagaagt aaacaaaatc gttcccggat atatttgcat tagcttctct
     2701 aaagcttcga tcgttgccat atcaataaaa ttagtcggtt catctaacag caacacatta
     2761 cttggcttag taaaaagcaa agcaatcgtc agacgggtcg cttctccccc acttaatgta
     2821 cataacggtt tcgtcaaagt ttcctgagca aaccctaagt gattcagcaa actacggaca
     2881 agcgattctg aaaactctgt ttgtctcatg acaaaacgaa tgatggattc ttcagatgtc
     2941 atttgataac ccttttgctg atatacttga aagcttactt tcggagagag aaggattcct
     3001 tctccttgtt tgcggatatg ttctaataag gttgtcttac ctgaaccatt ttcaccgaca
     3061 atcgcgattt ttttacctaa agaaaactga aaatcaccat ttaccagtaa tgttctccca
     3121 cttctttcca attggacatt ttgtcccatg atgggatagc ggctgtggag ttcaagagat
     3181 tttggtactg gaaaacggat ttgtttcatc tgctgcggct tggttgtttc tcctattttt
     3241 tggagtcttc tctctaatgt tttcgcattc ttttggatgg ctttttgtac actgtctttt
     3301 tgtttagagg aagacaaccg atccggtctg atttgttgct ttttcttttt actggacact
     3361 ttttccaact tttccgcttg ttttttcttt tcttggatcg atttttccaa tcgttttttc
     3421 tctttcatga tcgactctgc ttctcgtagc tgagtctttt tctccaattc cttttgagtg
     3481 agataggcac tataattccc cgaaaactct cggatttctc cgtcttttac ttcccagatt
     3541 ttcgatgcta gctcatctag aaaatagcga tcatgactaa caaaaaccag cgtaccataa
     3601 taataacgaa gctcttcaat cagtcgatca atactttttc tatctagatg ggttgttggc
     3661 tcgtctagaa gcatcccttg ttcataagaa gaaagaagac gtatcaactc gatctttcgt
     3721 tcttcccctc cgctccattt ccgctctgaa gtttcttcca gtcttaactg gaaatgactg
     3781 gctaattcca aatttaggtc ctttgcttca gcgagatagg ttaattgagg caggtagttg
     3841 aaggtgatct ctgtttggac tttcccatga tcgggaaaaa gttcaccggc tattagtttg
     3901 agcaaagtcg atttaccgac accattttct cctatgatcc ctatacgatc ttgctgatag
     3961 acacgaagag aatcaataga tagttctagc tggttgccaa agctgacttg tagatttgtt
     4021 atatttactg ctaaattttc catatgtttt acactcctat ttcatgtaaa acaacaatta
     4081 tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa actgcatcac atcttttcct
     4141 actctgaacg aaataactat gactaaatca ccaaatctgt catagctttc acctattcta
     4201 ctgtcgttcg aaaaaatgga catactccat ccatgaaatt gttgtttata catgtctttt
     4261 gaatttattt agttgttatt caaaagttcg aaacgtaatt tcatcgatgc agtcattttt
     4321 ccgacctcct tttttattag gttaccacac acgattcaaa aaaagcaata gctactgaat
     4381 agtaactgaa aaccttttat tcgaacgtag tgatcttttc tggtgctaaa cgaacagact
     4441 cgtaaccttc ttctaccgct tttcctattg ataaaataat aactggaaca taacggtctt
     4501 tatccaaacc aaatgcctct gccaactgat ctgcttcgaa tccaccaatt ggatttgttt
     4561 catagccatg atcacgcgca actaacatga attgcatagc cgctaagcta gaatcgactt
     4621 tgactacatc gttcatttct tctcttgaaa aactcttgta ataaggaatg atcgcagcta
     4681 attgttgatc tctgacttct tttggcatct tccctgattc gtaagcttgg ttatagattt
     4741 cttcgccata ttcgtaacat tgcatatcgc caaaaatcat gaccattgca gaagaagtat
     4801 cattttgtct cgtattgaaa cggatcaaag gacgcaagat ttcttttgct tcctcgcttt
     4861 cagcgaccac aaagcgccat ggttgcatat tgacagaaga aggtgcagtc gtcgcttctt
     4921 tgatcatttc cagcatttct tcgtgtggga ttttgtatgt ttcatcatat acgcgaacgg
     4981 atcttctacc agtcataatt gttgaaaaat catttttttg ttccattatt attaacctct
     5041 ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt tatctaaaag tgttagaaat
     5101 tgtttcattt ctacatcgct gaaagccggg gatagcacgg aacctgtatc tttatggtcc
     5161 atttcacaat gctcgatttt cttctgggct ttttctgtta gcgctacata cacttcccgg
     5221 ttgttctgag gatttctctg acgcgaaaca tagccatttg cttccaatac cttcagatga
     5281 cgagtaactg ctccttgatc aatctgcaac ttttcttgca aactgctttg tagcaatgga
     5341 ttttcttctt tcagtctcat catgatctcg tatctagtaa gacttatacc gatattttct
     5401 tcaaagatct ggacgatttt ttgatcggct aatttgattt gatataaaag gtgactaatc
     5461 tcttctaaag acataatttc ctcctttcta ataattgact cgtcaacgat tgacttgtca
     5521 ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg tgtaataaaa aaaacgctct
     5581 caatagaaaa gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa atgataaaga
     5641 aagcacaacg ctaaaaccaa gattattgat tcttatcaaa ctatagaaga tagctcttct
     5701 gcagtagaaa attttacacc gccaaaaatt tccgaaatcg ccttttgcaa gaatcccgtc
     5761 gaatattaga aattggatca gtatgcttaa acagtaataa aaaagttatg acaagatgtt
     5821 gtcacaacct tttttgagta aacggtgttc aaaaacttgc atctgcggta ataagttcaa
     5881 ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt cttactactt gatgcagggc
     5941 agctttttca ctacctcttt ttaataacca taaaatccta ttccttcata tctccagccc
     6001 atcggtgtta gccaatctct ttccttagca ctcaaggtat agtgatgggt accagttttt
     6061 tcatttggat taaatagtcg ataaattggc ttggaaccac cagaataaaa acttagacct
     6121 tcataacgcc aaccgatttt tgttaaagag ttgatctcag attgtgccat tgtgtaatga
     6181 tggtctccac tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt tggtgcttgc
     6241 caagcaatcc cttcatattc ccagcctaca gttggcagat aatttttttc tccggcattc
     6301 aatgtatata aatgttctcc tgtatgacgg ttatacagac gatacatcgg agaagttgaa
     6361 gttggtgctg tatcatactg tgtcagatta taggtttcga ttacattgtt caatgtagaa
     6421 ccataattag gcgcagtggc ataacgacct gttagccaag ccgtagcatc tttgtaagag
     6481 ttcgtattgc ttttccatgc accggaatag tagtaaacgc ctggctgaaa agaagtcgtc
     6541 ttcaataccg ttacgttatc ttgcaatgat tctttgtacg aaggatattt tctaaaagcg
     6601 gcattcaccg taatccattg cccattcaca tattccgaag taggcatcgt aacagattgg
     6661 ccattatagc ttcctttaat cccaaataga ttgtaattag gggcagaagc caaagtactg
     6721 ttcccccagc cactttcaac gatggcttga gcaatcatga ctgatgcata taagtcattg
     6781 gcacttgcca aactttgtgc agttgaggca atactatcaa tgaacgcttg ttgagaatta
     6841 gcagatgagc gagcataaaa agcatagcca gtatagtttt cgaattcaga caaatctgct
     6901 tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc ttctgcattc
     6961 tctggattgt tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg ttctgttgtt
     7021 tcagttgttg gttcttcggt tgaaattgta ttttctgaga cagtagctga ctgcaaagta
     7081 ctctctgtat cgttttcaat tcctgtttct gaagaactcg atgtctgagt tgcgacagtt
     7141 tctgtcgatt cttcagctag cactgttgtg ttggcaactg ctagatttcc taaaagaata
     7201 acgctaaata ataaattctt tgctttcatc ttttttctcc tttgaattat gaaacttttc
     7261 ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt aaataacatt
     7321 tgtttctttt tgataaattt gctttttcta cataaaaaga cgtaaatgaa gaatattctt
     7381 ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt aattctcttt
     7441 ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc atgttactga
     7501 cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata tcttcggagg
     7561 tcaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg tcgctgatgg
     7621 tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg atgttatttt
     7681 gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt gttccgcaat
     7741 aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa atgatatgcc
     7801 cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac acacccatga
     7861 tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat ggagcggttg
     7921 gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc atcgctaaat
     7981 cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata tatagctcag
     8041 cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca gctataacta
     8101 gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg cttgaagcgc
     8161 ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc tgttttgaaa
     8221 aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata atccgttcga
     8281 caggtttgac gattttggca gggattcctg caacgatggt attgtctggg acatctttcg
     8341 taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct ggcagaatag
     8401 ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact gacaagcctc
     8461 ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga ggaccaatca
     8521 atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga cgattgataa
     8581 agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc agcagaacag
     8641 tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg atatctgctg
     8701 gttcatggta cccagtattc agcgctgcta aaggctcttc gttttcagct tggatctgat
     8761 ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt tgtttttcga
     8821 tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg gcttatcatg
     8881 tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg gtatgctttt
     8941 ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta gcagtagcta
     9001 gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct actctctcaa
     9061 aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcgg ggctcccgag
     9121 agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt
     9181 tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga gaaatctaca
     9241 ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat tcgttgatca
     9301 aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat atcatagaca
     9361 aactggatag cggattactt gattttggac tcgtaatcga accgacaaac aaacaaaaat
     9421 accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat cgaaatcatc
     9481 ctttagcaaa gcaaaccgtc atccatccgc aggaactgaa aactgttcct ttgatcattt
     9541 cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc atagatcagt
     9601 tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc gataacggga
     9661 cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa aaaatattca
     9721 tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa gaccagcctc
     9781 tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt taagtagagt
     9841 ttttttcctt cgctttcagc tttttaacaa cgaataagat aagcaaaaga tccaacgatt
     9901 aaataacata agtttcttaa tttatttctt ccttttattt ctgataattc cagtaaatta
     9961 ctataattat aaaaaaagaa ttgacaaaac ataactgtct atataaaatt gattacgtta
    10021 acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct gaggaacatg
    10081 gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa
    10141 acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca
    10201 aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa aagggattgg
    10261 atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga tatttgtcat
    10321 ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg aacgcttttt
    10381 atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga
    10441 acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca ttaataacgg
    10501 tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg atgaattcag
    10561 tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat atacttatga
    10621 cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga acaataacca
    10681 tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg aatcagaatt
    10741 ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg tgaatcattt
    10801 acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca cggaaatcgt
    10861 agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt
    10921 gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact tggccatcca
    10981 tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa
    11041 aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt tgcggtctta
    11101 cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag taacgacatt
    11161 gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc tttgctgttt
    11221 gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt
    11281 ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc tactttattg
    11341 aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt atttattggt
    11401 attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc aaaattaccg
    11461 atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg
    11521 ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc tttagtttct
    11581 tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg cttcttcaac
    11641 cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg gtttgacgta
    11701 gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg cgtaacagga
    11761 atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg agcatatgct
    11821 atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat gctagcagca
    11881 gcttttgctt cattctttac tggtgtgaca gaaccacttg aattttcatt tatgttcgtt
    11941 gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc agtgagtgca
    12001 ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta cttcctatct
    12061 ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt agtaacagca
    12121 gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat gactcctggc
    12181 cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga caataaatat
    12241 gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt aacttcgatc
    12301 gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat
    12361 aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc
    12421 atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca tggtggtgct
    12481 gcagcacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc cggtgaaacg
    12541 atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac tgaagtttca
    12601 gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc agaaaatgga
    12661 gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa acatgctgtc
    12721 ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa cactgttgac
    12781 ttaaaaggcg aaccattcac tttatatgta gaagaaggtc aaaaagtcgc aagaggtcaa
    12841 ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac ggatatggtc
    12901 gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc acgtgatgta
    12961 aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt cttccccaga
    13021 cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc tcctttttcc
    13081 gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagctaa acgaaacata
    13141 tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc tttttcacaa
    13201 aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata cattattcct
    13261 gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc aatacttttt
    13321 ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg atcgctaaaa
    13381 acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca aacgctgcat
    13441 cacttgcttc aataatttct aactctttac ctgtcgcacg ctctaatgcc ttggctaatt
    13501 cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca ctttctacta
    13561 gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt tttccatttc
    13621 ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt aattcattct
    13681 ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta atcatttttt
    13741 cagtgaattg gtgatctggc gctaatatac tgatcgagtg atcggcatct gcaaaacttg
    13801 tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc ttatgttcgg
    13861 cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct ttcaacgctt
    13921 ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca gcttctttca
    13981 gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt ggaaccatct
    14041 tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt actaaataat
    14101 gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta catttataaa
    14161 ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga
    14221 caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc ctatattgaa
    14281 atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat cgaaacaaat
    14341 gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa
    14401 accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt actagatgtc
    14461 tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac aaatccaaat
    14521 tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa
    14581 aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa aatagccgtt
    14641 ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt ataattctct
    14701 ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta ttctccgggg
    14761 gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag cgctgaagtc
    14821 atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc
    14881 aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt
    14941 cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct cccatatatt
    15001 tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag gcgtaatctt
    15061 ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt aattcttcag
    15121 gcgattctgc taaagcgctt acaacaagat acgttccttt tgggaatggg atgattcgcg
    15181 tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt ttcccgttat
    15241 agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat gcatctattg
    15301 ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat
    15361 ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct ggtttttctt
    15421 cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca tcttacataa
    15481 aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta ttttttaact
    15541 atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa tcctctcccc
    15601 attttgtaaa agagatctcg ggagcttcga ctaacccata atctgaagaa ggaagccatt
    15661 ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct ttattaggaa
    15721 aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac ggattttctt
    15781 ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag cggtctccat
    15841 caaatccata agaaacgttg attacttgat ttggatataa atctcctaac ttatgcatct
    15901 cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga ttgactcctt
    15961 caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct ttcttttcga
    16021 tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag cgtggaaaag
    16081 atttttgtat gccgtatcta ctgatttctg aaggcagata tcctgaccaa tcacgaaaag
    16141 ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct gtcactcttg
    16201 atccatctag aagatcgcga ttagccaaag ccaatctacg gtttttgata tagtccatta
    16261 gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag attcctgtat
    16321 tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca
    16381 attggttcag atctttaagc atatttatct tcctccttga ttgccttttt atcatattat
    16441 caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta gaaaatgttg
    16501 tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt attatctgcc
    16561 tatctatgtt ttacctttag aaaaataaat tttggggaag acagattcta agtaaacaaa
    16621 gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg tacaaagcaa
    16681 attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga tacatcatac
    16741 ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt tttgtcagat
    16801 aaataaaaaa caaaaaaagt tccatcttta aaacacgcta ttcttttact ttttctcaca
    16861 ttctctaaga aaagaaaagg agaatgaatt tttgacaatt cgagcaaaag tgattatcaa
    16921 atatgtaaaa tcagctactt ttttcttaaa cgtttcttta tactaaatat aaataagtgt
    16981 ttcatgaaag gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga
    17041 acgcggttac agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta
    17101 cttgtccgca acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa
    17161 gaccaacatg acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc
    17221 tgctgtctta ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga
    17281 actctcgtcc cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat
    17341 tcttatagaa catccgcact ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat
    17401 aaattattca gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa
    17461 caaaaacagg aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt
    17521 tgaatcgctt attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat
    17581 tgctactctc gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga
    17641 ggactgttat tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca
    17701 aatgttctct tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta
    17761 ctctataaaa atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa
    17821 aatagataaa aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta
    17881 aacgataaca accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga
    17941 atcatgcaaa ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta
    18001 ttaggtacgc gttttgtttt ataagtcatg acaaatttcc ttcgttgctg gatagatttt
    18061 ccggcattgt tgatagattt tatgatactt ttctacattt tgtaagagag gttccgctgc
    18121 atctgtatat tggataaaat tctgacaaca actttcaggt gtgtcgaacc accctaaccc
    18181 tagtgcagca atcattgttg cgcctaatgc aggtccttcc tccacactta aacaatagac
    18241 cgttgcatcg aaaatatcag cttggatttg tagccatgca agatttttcg cgcctcctcc
    18301 caccgaaaca atacgtgtaa atctgcgacc cgctagttct ttacgtttca atcccaccat
    18361 tgacaaatgc tcattaaaaa tttagaatat tcacaagttt aacgacaatt atgaaaattt
    18421 atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa
    18481 agacacgtta cctaccgttt tcggttatat cggtattgga cttgcatttg gtatcgttgg
    18541 gaaagctgcc ggatttcatc cactagtcgt cacgttgatg tccctgctgg tctatgctgg
    18601 ttctgcccaa tttatcacag tcagcatgct tgctagtcac agcccattgc tttccatcgt
    18661 tttctcgacc tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta
    18721 tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag
    18781 tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat
    18841 gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact
    18901 tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat
    18961 gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca
    19021 gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag
    19081 caacttagtg attgtagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca
    19141 tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt
    19201 ctagggtcct accatttgtc ttactgaaaa aattcgattt accgcaaccg ttgcttgagt
    19261 atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc
    19321 aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac
    19381 ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggcagga attctttccc
    19441 tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg
    19501 cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt
    19561 gaacggacca ctaacaacga cttttcctaa catatgtcct gtttctacgt ctttggttgc
    19621 ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat
    19681 aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc
    19741 atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt
    19801 ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg
    19861 tcggacaagg tgcttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata
    19921 ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat
    19981 tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta
    20041 gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccggatcc
    20101 gttgatcact aagatttttt tcccgcgatt ggcattttct tctggagtta gaccaaattt
    20161 ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcag cttcttcatc
    20221 tgaaagattt tttggtgcaa gagcgacaat acgttcgtca actagttggt attcttgatt
    20281 gcttcctgct cttgttgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg
    20341 cacttcgtca cctacagcaa caactttccc gactccatca aaacctaaga tacgtaaagc
    20401 atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt
    20461 gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa
    20521 gctttttgga tcatcgatcg gcaacccttc ataaaaaccg actaccttca ttaattcctt
    20581 ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac agtgttcatt
    20641 tcaatatata tagtatgcta tttgttccac cttttttatt catcataatt attctcttac
    20701 ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag acttttgtca
    20761 caacctctta taaacggtgt tctcctacca actcttcggt cacaagtcaa aatattca
//
LOCUS       DACEMS010000042        20784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEMS010000042 DACEMS010000000
VERSION     DACEMS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.042"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1991)
                     /gene="treP"
                     /locus_tag="IW735_001375"
     CDS             complement(3..1991)
                     /gene="treP"
                     /locus_tag="IW735_001375"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1044385.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2214..2927
                     /gene="treR"
                     /locus_tag="IW735_001376"
     CDS             2214..2927
                     /gene="treR"
                     /locus_tag="IW735_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1044386.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3004..3450
                     /gene="msrB"
                     /locus_tag="IW735_001377"
     CDS             3004..3450
                     /gene="msrB"
                     /locus_tag="IW735_001377"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1044387.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3620..4222
                     /gene="ruvA"
                     /locus_tag="IW735_001378"
     CDS             3620..4222
                     /gene="ruvA"
                     /locus_tag="IW735_001378"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1044388.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4235..5236
                     /gene="ruvB"
                     /locus_tag="IW735_001379"
     CDS             4235..5236
                     /gene="ruvB"
                     /locus_tag="IW735_001379"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1044389.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5265..5843
                     /locus_tag="IW735_001380"
     CDS             5265..5843
                     /locus_tag="IW735_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1044390.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5895..6377
                     /locus_tag="IW735_001381"
     CDS             5895..6377
                     /locus_tag="IW735_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1044391.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6494..6868
                     /locus_tag="IW735_001382"
     CDS             6494..6868
                     /locus_tag="IW735_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044392.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IW735_001383"
     CDS             complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IW735_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1044393.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9167..9757
                     /locus_tag="IW735_001384"
     CDS             9167..9757
                     /locus_tag="IW735_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1044394.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9885..11234)
                     /locus_tag="IW735_001385"
     CDS             complement(9885..11234)
                     /locus_tag="IW735_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1044395.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11402..12142)
                     /locus_tag="IW735_001386"
     CDS             complement(11402..12142)
                     /locus_tag="IW735_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044396.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12155..13747)
                     /locus_tag="IW735_001387"
     CDS             complement(12155..13747)
                     /locus_tag="IW735_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1044397.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14315..14611)
                     /locus_tag="IW735_001388"
     CDS             complement(14315..14611)
                     /locus_tag="IW735_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044398.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14787..15512
                     /locus_tag="IW735_001389"
     CDS             14787..15512
                     /locus_tag="IW735_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1044399.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15505..16347
                     /locus_tag="IW735_001390"
     CDS             15505..16347
                     /locus_tag="IW735_001390"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1044400.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16350..16871
                     /locus_tag="IW735_001391"
     CDS             16350..16871
                     /locus_tag="IW735_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1044401.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17124..17687)
                     /locus_tag="IW735_001392"
     CDS             complement(17124..17687)
                     /locus_tag="IW735_001392"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1044402.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17938..18207
                     /gene="rpsO"
                     /locus_tag="IW735_001393"
     CDS             17938..18207
                     /gene="rpsO"
                     /locus_tag="IW735_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1044403.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18395..20521
                     /gene="pnp"
                     /locus_tag="IW735_001394"
     CDS             18395..20521
                     /gene="pnp"
                     /locus_tag="IW735_001394"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1044404.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
BASE COUNT         6784 a         3997 c         4195 g         5808 t
ORIGIN      
        1 gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag tcctgattgg
       61 tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat gtcagttttc
      121 ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc tggtgtgaca
      181 aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac agtatctaat
      241 cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc atgttttgtt
      301 tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt tgacggttcg
      361 acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac ttcagttatt
      421 tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac tgccgtacca
      481 gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc tgtttgaagt
      541 tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat tccctgacgt
      601 ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc gatgatgaaa
      661 ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac tccgatcgcg
      721 ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc gaccattgca
      781 gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc agttacgcct
      841 aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt cccacgatgt
      901 aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc aatcatcggc
      961 cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt ggtcatatgg
     1021 tggagaccag taatcactaa aggagcatac aggaatccga atacggcact aaacaaccag
     1081 ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc gactgtccag
     1141 ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga aaacaatgga
     1201 acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc tagatacact
     1261 aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat catctggact
     1321 tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc tacactataa
     1381 gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa tatttgtgta
     1441 gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg gaaaattgct
     1501 tcacccggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg ttcaacgatt
     1561 gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc aaggatcaat
     1621 ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac ggcaatagca
     1681 cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt ggaaacccct
     1741 tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt tcctatgatg
     1801 acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc ttcgatcgct
     1861 ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc acaatgacta
     1921 actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc atctgcttga
     1981 tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt gtttcttcat
     2041 ttactatact ttttttcgga gagagttgca agcgttttat taaaaagtac gagagaaaat
     2101 gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat tgaaatacaa
     2161 aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa aacatgaata
     2221 agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa tatcctccgc
     2281 attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca cgagaaacaa
     2341 ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa caaggaaaag
     2401 gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg actagctata
     2461 aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg ttagaagaaa
     2521 cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg cctgtttggc
     2581 atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc gattatctat
     2641 taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc tatgaatatt
     2701 ttgaaaatga gctttctttg acgattgctt acgcgcaaaa ggagatcaca gtagaagaag
     2761 taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca gtagtagtca
     2821 gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg gttcaccgat
     2881 tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata gttcaaaaga
     2941 aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa gggggcattt
     3001 ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt atcacccatg
     3061 gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa atatgatgat
     3121 ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt cagctctgca
     3181 gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac gaaaagacaa
     3241 gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg aagcaaagaa
     3301 gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg tggtctacgt
     3361 tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga tgaattaggt
     3421 tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg tacaatacgg
     3481 ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga aagaaacgct
     3541 ccccagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg tatgtgtgca
     3601 aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct ttatcaaccc
     3661 ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag gaaatcctta
     3721 ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg taatacgtga
     3781 agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt ttttgcgatt
     3841 agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa gcgaagacca
     3901 ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga aatttccagg
     3961 agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat tcggtgagct
     4021 gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg ctacagctct
     4081 atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa tcaaacgtgt
     4141 agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac gccaagcact
     4201 caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact tgttatcacc
     4261 agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc tgctttctca
     4321 atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag ctgccagaaa
     4381 tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag gaaaaacgac
     4441 gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga gtggccctgc
     4501 tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag gggatgtcct
     4561 gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt attcagctat
     4621 ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc cggttcattt
     4681 ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc tttctgcacc
     4741 tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac aggatctgca
     4801 ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag aaggggcatt
     4861 ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc tcaaacgggt
     4921 tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag cggatcaggc
     4981 acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga aattgttgcg
     5041 aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt cagtaaacat
     5101 aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc aaaaaggatt
     5161 tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac attttggtta
     5221 cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt gatacaacac
     5281 gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa tcttttcgaa
     5341 aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa tcgacaaaca ttttattatc
     5401 attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca ttgcattatt
     5461 tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa gtgctgaaag
     5521 caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca gaaattttac
     5581 aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa caaatggatg
     5641 aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt tcttacggtt
     5701 gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg atggaaatcg
     5761 ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt ctttatgcaa
     5821 aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa taatcgctaa
     5881 aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt tgccgttcac
     5941 cgatggcaga aggcttgctg agaaaacgaa tagcaataga aggaagggaa gcagaatttt
     6001 ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac cctggaacga
     6061 aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg caaatcacac
     6121 cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat gtagaagaac
     6181 tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta agtagcattc
     6241 ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt gatgagacat
     6301 atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgac gaaaataaaa
     6361 taacaaacaa tttgtaaagt tgacgctttc tttttaaggc gatgttagag catttccttg
     6421 tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct tagctaaagg
     6481 aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta cgaagttagt
     6541 tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag aacatgctaa
     6601 gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta ctgaacatag
     6661 tctagttatc ttacaagtaa gagcgaatcg tttgagtagc aagtatgaaa cgatctcagg
     6721 ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac aaaacgaaga
     6781 gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc tttcaccagc
     6841 aggggaacag gaacgattat caaaataatt tataccagat agaaaagcac gtatgagctt
     6901 gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata tcagtaaaac
     6961 ggaacgtttt aggatgagta tcacagcatg acagcattgg gttgattagt tgaatcgatt
     7021 ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct agcaggataa
     7081 ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat ataatagtca
     7141 ctagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagttttt ttgcgaaaac
     7201 tatttttata tcatatatat gtctaaaatc tatattataa aagaaatgtt gtttgatcag
     7261 cttctcttcg gtattagttc aaaacaaaac ggcaaagtat atggagctac tttgccgttt
     7321 tgtttctcta atactcaggt cagtttatag acgctcaata aacggtgttc aaaagccagt
     7381 ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag ccattttcac aaaatatttc
     7441 ttaatacttg aaactaagcg gctttttcac gacctctcaa taaacggtgt tcaaaagtca
     7501 gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc aaaaaatatt
     7561 tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt gttcaagaga
     7621 cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca atctgctact
     7681 tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac tgctggtacg
     7741 atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata taaaagattt
     7801 tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc aaaaatcgaa
     7861 gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt aaacaaagga
     7921 ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat caaaacaggc
     7981 aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg aaggatacta
     8041 gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa tgtttctgca
     8101 aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat aaatgctaat
     8161 aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt gatcccgcca
     8221 ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag caaagtataa
     8281 atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt agcgatttca
     8341 gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc aaatgctaaa
     8401 gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa agcatgctct
     8461 tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa aaaagcaagg
     8521 agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc aacataatct
     8581 aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac aatactgaaa
     8641 attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc aatctcgaaa
     8701 gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta aagcaagatg
     8761 gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac acctgagctt
     8821 ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt aaccaagaaa
     8881 cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac gggaaagata
     8941 agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag atagtcttta
     9001 aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt aacaaaattt
     9061 cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt atttaataac
     9121 gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga caaagtcgag
     9181 attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg tattagagat
     9241 caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata cattattagc
     9301 ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc gtatatttca
     9361 gatggcagaa cgaattaact accgagtggt ctggtcgaat atctttttgc tcttttggct
     9421 gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt tttggcctga
     9481 gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct tgatgaggca
     9541 aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa aggaccgtcg
     9601 aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt ttgtaccgtt
     9661 gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct ataaaaaagc
     9721 agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg acagaagctt
     9781 ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaattc
     9841 cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt cttacgttca
     9901 tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag taataaagcg
     9961 attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta aagaaatacc
    10021 agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt gccgaggata
    10081 cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa gaaacaagta
    10141 gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt caagataaca
    10201 gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg atgtgttgtt
    10261 aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag catacgtcct
    10321 gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac taaattgatc
    10381 aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg actgttatct
    10441 ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc taagtaaaat
    10501 aagatgatcc tgataggaat attattgata gcttccggca acactttttc aggattttct
    10561 gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt taccatttgg
    10621 aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga ccatagattc
    10681 gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata agctcctacg
    10741 aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc taactcacca
    10801 aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat cagttcagga
    10861 agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc tgttaaatcg
    10921 gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa agccgctttt
    10981 ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt tgataacaga
    11041 agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag ataggcgagc
    11101 aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa ccccgttcca
    11161 atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg ctgcagttct
    11221 tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt ccatattaaa
    11281 agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac cttttatgag
    11341 taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc agctattgat
    11401 cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc ggatggacaa
    11461 agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa atgacacggt
    11521 cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc aagccagtat
    11581 gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct aacgccgaag
    11641 taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca attgcgacac
    11701 gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga tccataccaa
    11761 ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt ttcaggacag
    11821 ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg ttgaaggatt
    11881 ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga gctaatacat
    11941 tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg atacagcgca
    12001 agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca cctttattga
    12061 ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt agatgtttga
    12121 tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt gaattgtcga
    12181 tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc gcccgcagga
    12241 ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta aatgtttgga
    12301 agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca gctacgccga
    12361 ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct gttgctggca
    12421 agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca tgggtcatac
    12481 caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc ccacgaacga
    12541 tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca atcgttcggt
    12601 tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct tgtaccatca
    12661 tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg attttttgga
    12721 agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca atcagtaatc
    12781 caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt ccagcacctc
    12841 gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg atgccaggtt
    12901 tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata attttggttc
    12961 gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc gggtcgcctt
    13021 tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca tctgcaccgc
    13081 tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct tccggacgtt
    13141 cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct gagaaattat
    13201 ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag tggaatgtat
    13261 ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct tttgcatatt
    13321 tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg gtaaaatcaa
    13381 tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg atcttgcctg
    13441 attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat tttttcccta
    13501 aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa ctattttggc
    13561 cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag ggtgcatacc
    13621 cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca tttgccagag
    13681 atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa accatatttt
    13741 ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga tgaaatgcgg
    13801 taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa ggccgctaaa
    13861 attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca ctgctaagac
    13921 agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag tgcacatttc
    13981 ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt taatgattct
    14041 cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac aaaaaaagtt
    14101 tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa atactgataa
    14161 atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc tattcattgc
    14221 aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag aactagaaca
    14281 gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc tttggctcac
    14341 gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt agataaatca
    14401 aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat gtaatggcta
    14461 aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca ttttctttgc
    14521 gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata caagccagaa
    14581 taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt atacagcagt
    14641 attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt caaatgccca
    14701 taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc gagtatgggc
    14761 tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa gcagcaaaag
    14821 agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc acaccttctg
    14881 gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag ccggaatact
    14941 tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt gtcaaaaaga
    15001 tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat ctagcgaaag
    15061 aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag gctaaaagcc
    15121 cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa tgtcaacaaa
    15181 cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga ggacaaatac
    15241 gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag caggcggctt
    15301 atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa gttcatccgt
    15361 tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc aatcaaacgc
    15421 ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt gttttcaaaa
    15481 aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga aaaaatgatt
    15541 gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat gtttgcaaga
    15601 agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca attgttgaaa
    15661 gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa ttttgattac
    15721 ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac atttttagat
    15781 gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca gctttacacg
    15841 gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc aaaacccatt
    15901 aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg tgtcactcta
    15961 ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc cgacaatgta
    16021 gtgattgcag gaaatcctgc tcgtatcatc aaggcagtag aggaagaact aacagaagag
    16081 tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttggt tgctttatta
    16141 gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga aaaccaagca
    16201 gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct gctgaaaaat
    16261 aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca ctcgcgcgaa
    16321 taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg cgaaaacaaa
    16381 tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt gcgaaaactt
    16441 ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct gcaggtaaaa
    16501 gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat actgggcaca
    16561 tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg agactaatcg
    16621 ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac ggtgagttag
    16681 caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga cgttatcaaa
    16741 aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat acgatccgct
    16801 acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca ggaatcctct
    16861 acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat catctctgag
    16921 ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc caatacacag
    16981 aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg gagaaacata
    17041 aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct tgtagcatag
    17101 tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt tagtgcaaat
    17161 ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc gatttcatgt
    17221 tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc attcatatca
    17281 taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc tcgatcaaca
    17341 gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct caaaatttta
    17401 ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg acttggtacg
    17461 tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc aacaccgcct
    17521 cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa ctcaagcatc
    17581 tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt tgcaacttcg
    17641 cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga atatgtaccc
    17701 ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca caaaaatgga
    17761 aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta cttgattttc
    17821 aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc cacttggtta
    17881 agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt gaaagaaatg
    17941 gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca tgaaggggat
    18001 actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca cttgaatgaa
    18061 catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa aaaagttggt
    18121 caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta ccgcgaattg
    18181 atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt cacatgaatc
    18241 tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta gatggactcc
    18301 tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt tgtttagtag
    18361 cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag tattcaaaac
    18421 gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac aagcaaacgg
    18481 agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg cctctaaaga
    18541 agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa tgtatgcagc
    18601 agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg aagcaacatt
    18661 gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct tccgtaatga
    18721 agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag ctatggcagc
    18781 aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg gcccaatcgc
    18841 aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag tagaacaagc
    18901 agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca atatggttga
    18961 aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt ttggttttga
    19021 cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag ggaaagaaaa
    19081 aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct ttgatgcttc
    19141 ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg aagataatat
    19201 cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag gtcatgaaga
    19261 tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa aagatgtcgt
    19321 acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg atgaaatccg
    19381 tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt tgttcacacg
    19441 aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc accaaatcat
    19501 tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt tccctcaatt
    19561 ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg gacatggcgc
    19621 attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc cttatacgat
    19681 ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa gtatctgtgc
    19741 tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg ccggtattgc
    19801 gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc aagggctgga
    19861 agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta tcactgcttt
    19921 acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag cattaactca
    19981 agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg ctgctccacg
    20041 tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac ctgctaaaat
    20101 caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg aaactggtgt
    20161 gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg cagaaatgat
    20221 caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg gtgaagttta
    20281 tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga tcaaaggaaa
    20341 agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag tcgaagatgt
    20401 tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac aaggacgcgt
    20461 aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg aagaaaaata
    20521 atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga caataattat
    20581 tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg aataaacggt
    20641 gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacatt aggagctgtt
    20701 tttacttggc tgttcttatt acttgatgca gaacagcttt tttacaacct ctccaataaa
    20761 cgatgttcaa tcagctgtcc ctcg
//
LOCUS       DACEMS010000043        20721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEMS010000043 DACEMS010000000
VERSION     DACEMS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.043"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            121..360
                     /locus_tag="IW735_001395"
     CDS             121..360
                     /locus_tag="IW735_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044405.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            427..666
                     /locus_tag="IW735_001396"
     CDS             427..666
                     /locus_tag="IW735_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044406.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            793..1482
                     /locus_tag="IW735_001397"
     CDS             793..1482
                     /locus_tag="IW735_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1044407.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            1488..2654
                     /locus_tag="IW735_001398"
     CDS             1488..2654
                     /locus_tag="IW735_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1044408.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            2736..3407
                     /locus_tag="IW735_001399"
     CDS             2736..3407
                     /locus_tag="IW735_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1044409.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3460..5436
                     /locus_tag="IW735_001400"
     CDS             3460..5436
                     /locus_tag="IW735_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1044410.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            5449..6201
                     /locus_tag="IW735_001401"
     CDS             5449..6201
                     /locus_tag="IW735_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1044411.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            6217..6978
                     /locus_tag="IW735_001402"
     CDS             6217..6978
                     /locus_tag="IW735_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044412.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            6991..7251
                     /locus_tag="IW735_001403"
     CDS             6991..7251
                     /locus_tag="IW735_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044413.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            7248..9338
                     /locus_tag="IW735_001404"
     CDS             7248..9338
                     /locus_tag="IW735_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1044414.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(9374..11431)
                     /locus_tag="IW735_001405"
     CDS             complement(9374..11431)
                     /locus_tag="IW735_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1044415.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(11576..11767)
                     /locus_tag="IW735_001406"
     CDS             complement(11576..11767)
                     /locus_tag="IW735_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044416.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            11955..12410
                     /locus_tag="IW735_001407"
     CDS             11955..12410
                     /locus_tag="IW735_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044417.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            12407..12649
                     /locus_tag="IW735_001408"
     CDS             12407..12649
                     /locus_tag="IW735_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044418.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            12667..12969
                     /locus_tag="IW735_001409"
     CDS             12667..12969
                     /locus_tag="IW735_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044419.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            13038..15092
                     /locus_tag="IW735_001410"
     CDS             13038..15092
                     /locus_tag="IW735_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044420.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            15092..17704
                     /locus_tag="IW735_001411"
     CDS             15092..17704
                     /locus_tag="IW735_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1044421.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            17701..20079
                     /locus_tag="IW735_001412"
     CDS             17701..20079
                     /locus_tag="IW735_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044422.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            20140..20682
                     /locus_tag="IW735_001413"
     CDS             20140..20682
                     /locus_tag="IW735_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044423.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
BASE COUNT         7376 a         3242 c         4156 g         5947 t
ORIGIN      
        1 agaataaaaa ctggaggaat aaattttaca gataaaggct tagaaggagt cacgacgaat
       61 aataaaaagg tagtattacc ttaggcattg agtaaggaat ttatttagga ggaaaattag
      121 atggcgcgac cagttttaga ggcgatggat atggatactt tgacaaaagg aaggtatgta
      181 cagctgttag ctcgtggtta ttcacctaaa gatgtattca aatccctgtc taaaggaaca
      241 gatatggaaa aagaaagatt acgaaaagaa ttcgattatt ggagaaccca taatggaatc
      301 aaggatttga aaccagcacg gccagtaatt aaacgaaaga aaaagataac ccaagactga
      361 agtgtagagc gtatgtgatc ataggctttt ttatttttgc aaaaaacaag aaaggtagga
      421 aaactcatga attatgaaga aatcgaaaac agaaaaaaag tgtcgaaaga aatggaagaa
      481 aagttgctaa aaatgatgaa acaaaaacac cttaaaagat tatctgtcat gcaatatatc
      541 aatgatatga agataacagg aaaagagaaa gcttgtttgt taggttcaat gaaaaacttt
      601 gaacagttga ggcgaactta tgtaaaaaca ggttccaatt gccaactatt attagaagtt
      661 tcttgacgat tatgatatga aaatgatcaa ttatgatgtg gcattgttat tgaataaaaa
      721 gaatagtaaa atttaacatg gtttatcttg gttagatgta ggtaaattaa ttcatatata
      781 gaaggtagat agatgaataa aaacaataaa actttattta atccttacgt gggcgtaatc
      841 attgtttttc ttctttttag ttattttgct tatcaaggtt atcaaaagga caaaatcgct
      901 gaaaaagtag atggtgtaac aaaaacaatc catattcagg aacaggctaa aacctatcaa
      961 gataatttaa ccagtttaac ggatatttct aagagacaac tgattactaa tcaaaaacag
     1021 gcattagaaa aggatctcaa aggtgaaatt gctggaaaac ttgttttttc ttctacagga
     1081 tttattctcc ctgtttttca aggagcgaat agcgacacgc ttagtttagg agtagcttct
     1141 acttattatc tagatactga aatgggaaat ggaaattata ttcttgctgg ttataacgta
     1201 gatacaccaa gcgttttatt atccgatatg actgatttat ctgaaggaga agaaattgat
     1261 ctaatcgatg ctcattacaa gtatcagtat gaagtctcca aaagaggaaa aatttctgat
     1321 tacagccagg tagttcaatc taaggaaaac aattcctttt tatccttgcc aaaagcaggt
     1381 aaaaaaccac tactcacact gttagccttt caagcaaata atatgaaaaa gtgcgatgtg
     1441 atccagtgtg aattagtcaa tgtagaaaaa atcaataaat aagggaagtg agtcacattc
     1501 aagaacttgt agaaaaattg atcaataaaa tgagtgccat ttttctgtta ctgttggtat
     1561 ccattctata tttaatatgg acggtggggt taaatcctcc tacgatatta tttatttatg
     1621 ataatgctgc acagttagtg acgtttttcg taggctgttt gtttcttttg aatctgtaca
     1681 aggtaaagag aaacgatcta ttcttcattg cagcaggcat tcttttttac cttttctttt
     1741 ctttttttag aagctaccga ggagatactt tctatggtga tctgatcgtt ccgacaatta
     1801 ttgctattat tttgattgta aaatggacga aatttactgt aattgatcgt agcttgtatc
     1861 ttgtagtatt ctatgtcttt ttactcgtaa cggtttatcg agtatttact gagataccag
     1921 taccagaagg acaaagtatt tggaatccat cgaacaaatt atcagatatt tggatcaata
     1981 caaatacgat aggtagttct ttaatgacat tagcccttct tatcacagga tttgctagtt
     2041 catttggaaa atggtacatg cgattgctat cattaccagc gttgattctt gctggggcaa
     2101 cgatttgggt ttgccaatcg aaagcagcat tatatgcgct tgttatcttc attatcttag
     2161 aaattttacc taagaaggtt tttcgaaata cacacttgtc cttttggatg tatgcagcag
     2221 tttcggttct agctttaccg atttcttatt ttgctgcaat gtcagacaaa gttcacttat
     2281 ttacaggacg agaagaaatt tggcgcaaat tctatgaaac attaggcgaa aaaacaggac
     2341 aaatatttgt agggatgaaa cctttctttt tccatagagg aaatcagatt ctaggtaatc
     2401 ataatacgta tcacgcactc gtcaatcact atggattgat tggttttgtt ctatcgatta
     2461 ttcttttgct tagtttctta tggttgctcg taagaaaacg tgagctgaca aatggacaac
     2521 taacattctt gtgggctttt ttagcgatca tgtgtcaatc gttcatggaa gaaacattaa
     2581 cgacaatcag ttgggtgcca attgtattct taatgttagc aatggctaca caccaatata
     2641 aaaccaccag ataaatgaaa tagaatagta ataagagaaa acactttttc gttgtgaaaa
     2701 agtgtttttt gttttgtctg aaaggaggca gatggatgaa aaaatggtta tttggttttt
     2761 taggagtcgc tttaatcgtc gtttgcagtg tgtttggcta tgttagctac caaaaacatg
     2821 aaggagaggt gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa atcaatgcac
     2881 acgcaaagag ttataaggag gatgcaacga acgtcaataa tgatatgtct ttggggcaaa
     2941 tgttgtccat acaaaaagaa gcaatcgaaa tgggcgtcaa taaacaagta tttgcacaaa
     3001 ttcagattcc tgcacttggt ttagcattgc caatttttaa aggggcaaat caatacactt
     3061 tgagtcttgg agcggctact tatttttatg aagatgccga aatgggtaaa ggaaactatg
     3121 tcctagcagg tcataacatg gaaatgccag gtgttttatt ttctgatatt caaaagttgt
     3181 cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac aaggtaacgc
     3241 gtaaatttat cgtacctgag tactttaaat taatcgatgg cgttcctgaa gagaactctt
     3301 tcttgtcttt accaaagaaa ggagagaaac ccttattaac gttgtttact tgtgtttata
     3361 cgtcacaagg aaaagaaaga tacgtggtac agggagagct acaataacaa gaatttaatc
     3421 aacgcgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa tcggtatgta
     3481 aaaaaagctg gaatgattgt tggaatgtta ggaatcattg caggctgctt tcaggggtat
     3541 cgagcttatg cagaagatgt cacgcaaaaa acgccaccag aaagggtcaa tatcacggtt
     3601 cataaattaa tgtacgatca aagtacgcaa ttgaacgttg atattgacgg aatcaaaaat
     3661 gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa ggcagattat
     3721 ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgac tgaagattct
     3781 gatctaacca acgcgaaagt ggacgaaatc gttaaagatg tagaagacaa gggatctaac
     3841 agtgagtacg ttaagaatgc aaccaataaa attacttcag ctgtagatga aaatggtgag
     3901 atcacttttg ctgatcaacc agcctatgtc aattccaaag ggaatgtata tgtggtttat
     3961 gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt tgtgattgcg
     4021 ccaatgacag acaacacagg atcaggattt ttaaaagata ttcatttgta tccaaagaac
     4081 attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcac agctcaaagc
     4141 aaacaaacac ctttaaaagg ggcaaaattt gagttataca agggcgaacc tggaaaagga
     4201 acaaaattag gagagcttgt atcggatgat caaggaaagc tgactgcaac tgatttgacg
     4261 ttaggaaaat actactttgt ggaagttcct tcagaagttg tagttggatc agatcaagaa
     4321 ccaacagcag accaatattt gttaggcgca gacgctcgca acgatgcgca caataagtta
     4381 acctttgaaa ttacgaatga cggcgtgaca agtgatttaa aagcttccta cgtgaactat
     4441 aaagcaccag tgatcgataa aacagtgaca aatggaacag gagaagaaca ttctttccaa
     4501 attggtgacg cagtgaatta tcaaggtaca attcatatcc ctacagatat cgcaggtgga
     4561 acagatggga ttacagtaaa tggtgtaaaa agtgaaactt ctccttacag tgtatttaaa
     4621 tggggcgaca cagcaggtaa aggactttct tatgttgctg aaaaagcaga tattaaagta
     4681 acaaataatg atggttcagt cgtattaaaa gaaaatactg actataaaat cgaaaattct
     4741 gatagtggtt ttgtgatcga ttttatcgta aataatggac aagtaagtga tacagttgcg
     4801 aatttacatg gtcaagatct aaaaatgact tataacatgt ttgtgaatga tagtgctgaa
     4861 atcgcaaatc cattaacaaa tagcgtggat ttcgtttata acaataatcc atttaatcag
     4921 gaagaacacc acgaaaaaac gaaagctgac gttgtgactt atggcgcaaa attcttaaaa
     4981 gtcgatagcg gcctatttgg aacaggtatc aaagcaacac cgcttgaagg cgcagaattt
     5041 gcagctaaga atgcagaagg taagtactac ggtggactta ctgatacaga taaagatggt
     5101 gtcaaagaag cagtttgggt agacgatgta gcaaatgcag caatcctaaa atcagataaa
     5161 gagggtcatt ttgaaatcac tggtttaact gaaggagatt actcactgga agaaacaaaa
     5221 gctcctgaaa actatcaaaa attaacaaaa gagatctctt tcaaagtaga caaagattca
     5281 tacaaagaag aaaaccgaat cacgatcaaa aataatcaaa aagcttccgt accaatgact
     5341 ggtagcaacg gtttccaatc ttacgtattg atctcttgct tgttgcttgg ggcaggcgca
     5401 atttctgcag ttgtttactt taagaagaaa gcctagaagg atcagctaat gattatcaga
     5461 caccctaaga aaaagcgaat catgggtaaa tggatcattg ctttctggtt actttcagct
     5521 gttggtgtct tacttttgat gcctgctgaa gcgagcgtgg cgaaatacca acaaaatcag
     5581 cagattgccg caatcgatcg aacaggaacg gcagcagaaa cggattcttc tttggatgtt
     5641 gctaagattg agttaggcga tcctgttggt attcttacaa ttccaagtat ttctttaaaa
     5701 ctaccaattt atgacggaac ttccgacaaa attttagaga atggtgtagg gattacggaa
     5761 ggaactggcg atattacagg aggaaacggt aaaaatcctt taatcgcagg gcacagtgga
     5821 ctttataaag ataatctttt cgatgatttg ccttcagtta aaaagggaga aaagttttat
     5881 atcaaagttg atggtgagca gcacgcttat caaatcgatc ggattgagga agtgcaaaaa
     5941 gacgaactac aaagaaactt tgtcacttac ttagaaccaa atcccaatga agatcgagta
     6001 acgctaatga catgtacgcc taaggggatc aacacacacc gtttcctagt gtatgggaag
     6061 cgagtaacct ttacaaaatc agaattaaaa gatgaagaaa ataagaaaca aaaattatct
     6121 tggaaatggc ttttaggaag tactgttttt ctttcagtca tgatcattgg ttcgctattt
     6181 gtttataaaa agaaaaaata aaggagggta accaacatga gaaatagaaa atggctattt
     6241 gtttgtgcag ccgctctttt ttctttcact gcctacacac agttgacgtt tgcagacaga
     6301 caagaaagca cggatagcac agaacaaaca atccaaacgt cagatagcag cacgattcaa
     6361 agcagcgatg aaaccgtcga ttcgtcaaca gttgattctt cagaagaacc taaaattgct
     6421 gaagacgatc aaatagtcaa acagaccatt cacgttcaaa aaattatttc ttccgaacaa
     6481 ttagacaacc aggggcaaaa gcttgaaaaa caagaaggag taaatggcgc gaagttttct
     6541 gtttatgatg tatcggacat tttacaaaag atggatgtta aggatctcac tactgaccaa
     6601 attgaatccc aattaaaaga tcgagtgaaa aaactatcca gtgatcaact gaagctaatt
     6661 tctaatggag aaacaaagac aattgatcaa caaacaggtg tctttgaatt ctcggtagaa
     6721 gtacaggcaa atcagaaaca agcttattat attgtcaatg aatcaagtcc tgaaaacatt
     6781 tctaatagcg aggatatttt gctattaaca cctgtttctg ataaaaatgg tgaattctta
     6841 aaggatgtgt ggatttatcc taaatcagaa gcttcgcaac ctaaagaaga agtaaaaaaa
     6901 atcgtatcta ctggcgtaaa gaaaaacttc tttgagaatt gttgggattt tgttactcac
     6961 ttgttttcta gagattaagg aagaaataaa atgaaactga tcaaattatt attattcaat
     7021 ttttcttgtt taaccgcagt ctttttgtac tgcggtttat tattttctaa actatctatt
     7081 ttgccaagct accaactata cctaatgagg attttaaaca taccagctgc ttccatgccg
     7141 ctagatatta tggaaaagct cattaggaat ccattattta ctacgatcaa tttgatcgtt
     7201 attgtcggaa tttcactagt catttataaa aaagagggga tgaagggatg aattttaaac
     7261 actcgtggac aagacggtta aaatatgttt ttcccataat gatgatgtta ggtacattgt
     7321 ttgggttaaa aacagccaat gtctttgctg aaaaagttat tgtcgatccg tcaaatccaa
     7381 tccagaacgt aaagaataat tttattattc caaaagatgt tcctaatgca aaaacaaata
     7441 ttactgtaaa cggagcgtca aaagtttaca aatatcatgt tgatgccaac aaaggtggtt
     7501 ttacagacga ctacgtagga ttagtggagg gaaacaaaaa tatacgctat ccttctttcc
     7561 aaaaagaata cggtgaaaca aaccttgttt tagaaggcgt aacaaccaac accaaagtca
     7621 atattgacta tggaaaaatc ggtacgtata acgggaaaaa agtcaatatc aagctagttc
     7681 tatctaatat tcacttatac tcggatacgt tgccgtggaa tctactagat aacaactata
     7741 caaaaactca ttttcgagac gatggctata aaaatacgaa tggtgcaatg agtaaatcta
     7801 aaaaaagaac agtactttgg atctcagaca atcttttctc aggaattgtt taccattcaa
     7861 cacaaatgaa tgtgcagcta gtagccacat atgaggacgg tagccccgtt caattttcag
     7921 gagatacatt tatcagtttt aactctttga accctgcagg aggaaaaagc acggatctta
     7981 aaggagaata tgcccactat gacaaaatga atacgacaga ttggtatgtc agaaaagata
     8041 cggttctttc agaatttaaa tctttctaca acaatctgaa cgtggtaggt ggccacccag
     8101 gaggaagctc taagctgaca caagcggata atgatttcaa taatttgcat gataaattag
     8161 gagatccgaa gtttggtcaa ggtactgtgt cctttaaaat atctgaagca aatccgacgt
     8221 ttgtgattgg ttcttctaat gttcaaacct ggtttacttt atcttctgcg acaatcttta
     8281 gtgttgttcc tgatcaacct gaaaaaacag gggttgataa aaatggaaat aacgtgaacg
     8341 ataaaatgtt gcaagtagga gacacgattc aatatcgaat caaacagaag gtgaatcgat
     8401 taggagtgga tttactagcg gtttacgatc gatttgagtt aatcgataat ttaccaaaag
     8461 aagtaaatta cgtcgatgca cacgttgaaa gtggtacaaa taaaaaattc gatgtatctg
     8521 gtgaagtgac ctatgacaaa acaaaacacc aggtaaaata tgcagcgaaa gctgatacat
     8581 taaaaaatcg aatgaagtat aacggcgaaa cttacgaatt agtgatcaat gtaaaagtca
     8641 atgaattggc caatcaaaat agtgtagcca aaaatcaggg aacgtcgatc attaataaag
     8701 tagaaaagga tacaaatatc attaccgttt atttccctaa aattccagtg aaagaagtac
     8761 aacaaaatgg aaaagatgta aacggacgaa atgacggtaa gaagggtacg ccgacagctc
     8821 ctttaaacgc aggatctgaa gtacaatatc tagtcacaca aaaatggcac acaaaaggcg
     8881 tagacgctgc gtcagatcac tacaaacaat ttagtattca agatccaatt gaagctcgtt
     8941 tgacatataa agaaggttct gcgcaagtaa tagataagag taaaggaaaa gatattactt
     9001 ctgaaggcac actaacctat gatagcaatt caagaacgct gaagtgggaa gcttccgctg
     9061 acttcttaaa taacaatctt ttagatggac gagaaattca gctgatattt acagctaaaa
     9121 ctccattaca atcagaaaaa aatattgata accaagccgt agtagcagta gataatgttt
     9181 ctaataaaac aaatgttgtg acaattgggg ttgatcctaa cttgccacaa gttattgttc
     9241 ctaaaacagg ttctacacat ttagtaacga ttttagcggt cagcttacta ttacttgtgt
     9301 tagcaacttt tggctacgca gttctgaagt tcaattaaac aaaaaaacaa ggctgaaaat
     9361 cagccttgtt tttctatttg aaaatgggat tggcactaat aaatacgttg tctgaagaaa
     9421 gtgatccgtc tgcagaaact tcataagcat gggataaacc aacgatactt tgattgttgt
     9481 aaagcaagtt tgcgttatga gcagcgctat ctttccacat aaccatagca tttactgctc
     9541 ctgtatctga aaggatctcg tcaacgtaaa ggtagaaact attgatattt tctcctttag
     9601 caccgtagca gcctaagctt tctaagagat cttggaaagt accgccgcca ggtcggtaat
     9661 gactgaagct tacttgtaat tcttttgcac gaatatctgc agcttgttga acgatatcat
     9721 tgaattgcat tggggcaacg ccattttgtt gacggtagct atttaaccaa gcaagttctt
     9781 tttgacgcac atagttgttt aaatcactta gtgagatatt tttgtatttg aaagtgatcg
     9841 ttcgattttg gtcaacatta ttgattacac gttgtccatt tttatcaacg gtaacagatg
     9901 aatcgtcatt tctacacaag aagtagtttt gatcggtcat atttttccaa gaagtagaga
     9961 aactatctcc ctcgtaaacc gtttgtgttt cagtagataa aacagcgttt gtgtcaacgt
    10021 ttatatggtt cacagtgacg ttgtattgat gagtataggc aaaattgatt ccttgattac
    10081 ttgtaacgtt gtttaaagtc acagtgttgc tagagctttc tctaggatca agaacaaaat
    10141 tagtgaacat aggtgcttca attgtgatgc tttgtccata aacgacggga taactcttac
    10201 tgtaattata gagttcttgg atatttggat cagtatccga gactttattt ggacgagctt
    10261 tcacttcaac tagaaagact tcatcatagt aaaaagtaaa tgtggtattt gtgttaatcg
    10321 tttgtgtttg tgtttcattc ccatttactt gatactgtgg tgcttcaaag ttagtcaagt
    10381 cggttctagc ttttgcagtg taacttgtac cagcttttac tgagacatgt tcttctttta
    10441 gcagcgttcc agtttgtcga ttcatatgac ggacagttac cgcatattct gaaggtttag
    10501 gtttgtgttg taacccttta aatgcagaat caagactatt tttagcatta tttacttggg
    10561 tttgagaagc tttagaatcg tctagtactt ttttagcatt ggataaagca gtctgaaaat
    10621 tattccaaga tccttctgta aaatctcctt tagcagttga ttttacttgg ttgtacaagg
    10681 cttgtaattc cgttttgttc acagtaggag ctggtttttt ctttaatccc ttgtaagcag
    10741 aatctaatgt gtttttggca ctatccactt gtttttgagt cgcttttgag tcatttaaca
    10801 cttttttagc attgtttaaa gcggtttgga atgcgttcca ggtattgtcg gtatagtcac
    10861 cttttttagt tcccttaacg ctcgtataaa gagcttctaa agctgattta ttcactttgg
    10921 gtttagaggt gtcagcacca taccaaccaa taccttcgta tttccagcca tagtctttgg
    10981 ttaaccattg tacttctttc caacttgcag tgtagtgatg cgaaccagag gtaacacctg
    11041 gaaggaaaag gcggtagatc ggtttattat ttttttcggc agaacgccag gcaggccctt
    11101 cataagtcca accgtaattt ttggtcaacc atttcacttc atcccaacta gctgtgtaat
    11161 ggtgatcacg aaggccttta ttgtataaac gatagacaag gtggccaccg ctagtcggtg
    11221 cttcccaagc ggggccttca taattacccc acttaatttt cactaggtga tcacgttctt
    11281 ttgtgctaga ggtgtaaaaa tgttcatggt tatttggatt ataaaggcga tacatgacgt
    11341 tagttgaagc agcgtcagca gatccagcaa gagcaactgt ggcagcaaat gttccaatag
    11401 ctataacagt ttttgggttt aataatttca tgttttcaac tccctattaa atgattaaat
    11461 attttgttta cattcataat ataccacggt ataccgaggt agtcaacggt ataccgtgga
    11521 aattttgaaa taaaaaagcc gccctatgaa gagtgtaagg atggctgttt atatgctatg
    11581 aagaaatgga ttcggcgtat ttggttagtt tggcagcatt atcaaatcgc attttttcaa
    11641 tcgcactttt tttctttatt aaatccgcaa ttgttgattc tgcgactgaa gaatgtttgc
    11701 taatttcata tgctgtcttg ccagtttcaa atagtaacca ttcgattttc tctttatcag
    11761 cgtacattgc aatgctccct cttgattttt tctttattat agctcttttt tgtaataaat
    11821 cacaatatca agaggaaaag tttctataga aaggtgggaa attatgagta tccgttcccc
    11881 gtgtatcaaa atgaggtttg ttgctacttt cgttatttaa tatactaaaa taaaacaacg
    11941 aaagaaggaa tagaatgaaa aagttaataa tggtatttac ttcgttgatt gccatgattg
    12001 ttattttagg tggatgtggt agtgaaaaaa ataagcaaga tagtcaagat aatggcgtgt
    12061 taaaaccagg aacagtgtgg aaagaagaag taggcggact tgttcataat ttaaaaatta
    12121 tagatgatac aacatgggaa tattccgaaa gtgtttggca tcctgaacaa gtacaaataa
    12181 cagttgagaa gctaaaagac tataaaggat tagaaagata taaggtaatc gattcaggtg
    12241 gagtagattt attcataggt aaaacagcct taatagttcc ctataacaaa gacggtttag
    12301 aaacgtttaa atttattcca acaaacgaaa aagaacatcg agataaaagt gaaatattaa
    12361 aagaatacag tactaaatct ggatacaagt tacaaaagac tagcgagtga ttgctagtct
    12421 ttttatttat cctgaaggag gaatcgcagt ggcaaaagct aaaacgccat ttgaaaaagc
    12481 cgcgtctaaa atcttagaac gtcgcggtgt atctgaaaca cagtggaaac aagaagtcct
    12541 tgaaaaagca caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta
    12601 tcaacgtgaa cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac
    12661 aattttatgt ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg
    12721 gttgatattt ttagagcatt acaaggtgca gcaactagtt taaaaaacaa tctgatgatt
    12781 tttgcggctg tagctgccgt tgcctccatt gttcttgggg ggattttctt cttctttggc
    12841 cgaagaggag ctgaaaacgg gaagatgcac gtaggttatg tattgatggg ggtaggagtt
    12901 atattagctg ccgcctctat tgtggtttcg ttatttggaa tgtttggaca aaccccacag
    12961 ggactataaa aaataaatag aaggagtaaa aagtacgtgg ctacttttta ctcctttttt
    13021 atgaaaggag aatactcatg tactttacga tttcaggcaa taccaaaaaa gataaagagt
    13081 taataaaaaa agcaacgaag aaagtgggaa agcgtactgc ctttttaccg aaaacaatta
    13141 aatattctgt cattgaaacg gaagaacagc tacatgtgtt aggacaagtg ttagaacaag
    13201 agaatcaatc ttctgttaaa ttgactctca cgttacatga taaagaagga aaaatagaaa
    13261 aggctggcac agaatacgtt tttggcccac tggattatca aatttcagaa ggtttttatc
    13321 ctacattgat ggatttgatc cgccttgatg tgtttaagga aacggatgaa accatgagtg
    13381 tggaagagtc ttatcgaaac gaaaaactgc tacaagtttt agaagaagag ttagagagcg
    13441 accaaagaaa agtgagtcaa gtgagtgaag tcgaagtaaa tgatgaagta ccatgggaac
    13501 gaccaatctt tccaaagaag gagtcaaaag aaaaagagac agtctatgtt gacgaacaag
    13561 ctgatattcc gactcagaaa tcgatatttg aggaagagaa cgatgtaatg ggtcaattgg
    13621 aggtagatcc ctttgaagaa acaatggagg atgaagagac ggccgaagta gaagttgatc
    13681 tagttgaagc taatgaagag ccaacatgga ctgaagggga ttctcaggaa tatattgaac
    13741 cacttatcag tgaagaagta gagaaaaacg aaacagagat acaaaaggca actgtatttg
    13801 attatatttt ggatcaatat ccgccagagc atgaatggat gaaagaaaaa aataaattat
    13861 ttttaaatga gctttattcg aaatatcaat taccccaaac ctatcaagca tttttagaga
    13921 gtaaagaaga attaatctca cagggtaaag gcacactttt agaacagtta gagtctgttc
    13981 aatcaacaaa ttggcatgag cttgcacaag aagatttatt agccactttt gaagaacgtc
    14041 aacaagaaac agatcaagaa ataaatgagt atgcgacgga acagtcatct aattgggaga
    14101 gaacaaaaga ggaactaaat aaagaagaac aacgagtgat tgaagaagaa gtacaaaaaa
    14161 ttaaagagcg atacgaaaag aaacgcgaca atgcgtattc cttgtgtata gagaaaatta
    14221 ataactttac taaaactaat caagaagctt tatcgaaaga aaaagaaaac ttgttaaaag
    14281 aacgtataaa agaacgaaaa gaagagttct accgaggttt acgtacagat aagctaaaga
    14341 ttagcaaaga attaaatgaa caactgaata aactttatca aacaacgact gaagttctta
    14401 tggaaagaca cgggcagatc cagcaagaat tacaagcaca aattccaaaa tggaaaaaag
    14461 agtatgaaat tcaacaacaa gaattgaaag aacaagaaga aaaacaacga caagaagaaa
    14521 aagagcgcga acaattagaa ctaaaaaaac gtgagcagca tcttgcagaa caaaaagtgg
    14581 cgatagaaaa acaacggtta gagcaagaaa aggaaaaaga agcagctgct cgtaaggaac
    14641 gtcaagaaca aatggataca ttgcgacaag cacagttgaa ttatctagca caacctcaac
    14701 cagtagctgc tgttcaaacg ccagtgaaca accaagcaaa tttaactcct tcaccacagc
    14761 gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt ctaggtggag
    14821 gaacagtatt tgcttttaat catatcaatc aatcttcaca ggcagattct gtagccacaa
    14881 cggcgtcaat caaggaagaa gcccaggaac aagcaagaaa agagtatgaa caacaattgg
    14941 aagaattatt gaatcgtacc aatcagtcaa cagcagagtc aagtatggaa aatagcaaag
    15001 agagtgatcc tagtaaggct aaagaaggat caagcgattc aactgtacac tcaaacactt
    15061 ccgaaagttc agaatcaaat gaggtgaaat aatgcgtatt agagaaacaa cgagacgaaa
    15121 agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc gctatactcc
    15181 ttcaggagaa gagctaccag aaaaacatga caaacgtatt tctcaaaaga ttttttatag
    15241 tggcttagct tatcttcctt taaactatgt tgttacaaga ataccagaag catttcatcg
    15301 atttgagatt attccaggaa gtttacaagg agtgaatatt tttcagttac aagaaatgcc
    15361 gcagttattc cattttgatt caactggttt ttggaatcct ttgaccccaa caacaagttt
    15421 aggcacacgt ttagttgttt ctacgatttg tgttgttcca gctgtactta tgggattgaa
    15481 aatggacatg tggtttcgtc cgctagcaga tgggcaaaaa ggagataacc gattaaaaac
    15541 gttaaaggaa atccaacaaa tttatccgca aataccagaa tcaaagggaa gttttcctgg
    15601 ttatggcgga ataccaatta ctcattataa aaaatactgg tatattcaga cggatacagt
    15661 aaatacttgt attgttggta cttcccgctc agggaaaggc cagatagaag ttttagcaac
    15721 cattgacaat ttgtcgcgtg cagaaagaca aagttcaatg gttgttaatg atccaaaaac
    15781 agaattatat gttgctagta aagatgaact agaagctaga gggtatgatg tgtatgcctt
    15841 taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca ttgtgaaata
    15901 ctggaagcga ggagacatag acaccgcaac acagttaacc aatactttta cgaatacgat
    15961 ttattatgat gcaaatgctt cagacaataa atactttaac gaaaatgcgc agaaagctgt
    16021 caatgcgctt atttttgcgt tattggaaca agcggatcaa acaggggatt actccaaatt
    16081 aacaccaaat aatctcgttg aactactttc agaaattggc ggttttaatt attatccaga
    16141 tcctgagaat gaattcaagc agctcaatgc cttagatgag ttcatgaatc agttaccccc
    16201 tggaaatata gcaaaaaaac aatatggggc aacaaaaatt ggatctgata aagcgaaagg
    16261 gaatatcttg tctacggcaa ttacaggatt gaatccattt accctaacga aaattgcgaa
    16321 gatgacttct caatcgacca ttgattacaa gtccattggt tttccgaagt atttggatct
    16381 caaattacat gaaagcctat taaatcaacg cattgtcgtg gaattttatc ataagggaaa
    16441 gaaatttcac gaggaacgag tcaaagttgg ctatcgtggt ttttgtgaaa tcaactttga
    16501 ttgtcattta gagcagggag atcatgtttg cttaaaatac aatcatcaag gaaaacaata
    16561 tgttgcttgg tatgagtttt ctcaaaaagt attgaaagac gaagagggaa aaatcgttta
    16621 tcgaaaaaaa cgaggagaaa ctcatttgcc agaactagaa aaagaagccg ttctaacatt
    16681 agatgagaag aaaagtaacc tattagtaga gtctattcgg atgcactata ccgataaacc
    16741 aatggctatt tttatgttaa cgcctgatta tgatccatct aaccatatca ttgttagcat
    16801 ctttcttagt cagttgtata aagaattatc tacgcaatgt gttaagacta aaggagataa
    16861 atgtcaccgt aggattcatt ttattttaga tgaatttgga aatatgccac cgatggacaa
    16921 catggaagga atcatgacgg ttacggcagg tcgtaatatg ttatgggatc tgtttattca
    16981 atcttatcag caactttatg cgaagtatgg agaacaaaac gcagcgatta tcaaagaaaa
    17041 ttgtcagaca catatttata ttatgtcgac agatgataat accatcgaag aattttctaa
    17101 aaaagtgggg aataagacgg ttgaacaaga gaacagtacc atcaattcat taggtatgaa
    17161 tactcatatc aatcgcaatg ttgattcaga tcgtatctta actcctgaaa gaatttctac
    17221 ttttttagaa ggtgaaacga ttgtactcgc tcctttaaaa cgacaagatt tgaaaggtca
    17281 taaaatacgg ccgtatccta tttttaatca tggggaaacg aatcttcctt atgcttatca
    17341 atatattgaa gatttcaatc catcgtggga tatcaatgat atagacatac attgtacaca
    17401 tgcggacttg gatcttaagg ctttacaaat tgattatgat tatttcctac cagatgaata
    17461 tgctagaaat ctctatcatg gactgcatga tcaattagaa gaaaaaaacg aagaaaatat
    17521 gttttataaa atcgttcaat cttttgagaa tgagacggct aaaaaattgt taattcaatt
    17581 ttacgaagaa caagcaacgg atcgtcttca agaatatgtt gaaaagttga tcaaaaatga
    17641 agaaatttct gaaatgatta ggaaacggct gttattggaa gctgaaaagg cggtgaaaga
    17701 atgatcattg gaggaatttt ctctggcata gccgactggt ggtcaggttt attcggagaa
    17761 ggggatgcag atcccaaaac cgtaacgaaa gtcttacaag attatggtaa ttatctgcag
    17821 tatcaagatg tattcagttg gatttgtaat acttttttat ggggattgat caaagggtta
    17881 tataatctca atactttatt agaaaaaaca atttatcaaa gctttgattt aaaagactta
    17941 ttgaacgccg ctggtgttaa tgaactattt caaagtttga ttagtaaggt attggctctt
    18001 ttttgtgtcg tcacattaat ttatttaggc ttgaaatttt ttatgagtaa acacccaccg
    18061 aaagtcaaaa atatcttggt taatttattt atggccatga tcttgatttt tggtggtagt
    18121 tctttaatcg atgaagggtt aaatatcagt aaaaactttt atggagacgt cactgcagca
    18181 aataaagcag ataaaacggg ttcacctgct tttcaaatta ttcaaaataa tgtgattgat
    18241 gtcagtacat tactagcgac tgacccgaat aaagtagaat ccttaccaac agataaaaga
    18301 aatgctttaa cagcgaaaaa tttcaaaaca gcgaatatca atgggattat tacacctgat
    18361 caagctaaga aaatgtctga atcaagtgat attagtacag tacaaaaaga tcggttaaag
    18421 gcattacagt atcgactaga gttagacgat aatgggaaag aaatccctgt tcaaattagt
    18481 gatgaaggtt tagcaaaata tgtgtatacg tcaggctatc gtcgatacat tagtagtcca
    18541 gggattattt tggctgggga atcttctttg tcggtagcct atttatttat tttgtttaca
    18601 atcgttactt gcattattga gctagtattt aaaaaattct atttagtaat tgccgcgtca
    18661 acggactttg agacaggaca acggatgaaa agtgcgatag aagatatttc tcaatccttc
    18721 ttgttattgg cgtttacgat tttagaatta cgaatctata cattaatgct ttcaggtatc
    18781 ggtgatttgc atgcggtagg aaagatcaat ggattcttgt atgttgttgc tttaattgtg
    18841 ctaactattg cgctattcaa aggctctcaa tcggttacga agattttcgg tgttgatacg
    18901 agtctgaaaa acggcagcaa ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa
    18961 aatgtgggta gcggagctaa aaaccttgca agtgctggta agagtggttt aagccattta
    19021 aatgatattc gtaagaatgg atttggccat cgaaaagata ttacccatga taacggggaa
    19081 gaacaatcag acgataaagc tacaactgta cctaatagta agaagacagt ttccgaaatg
    19141 ttcgggaaag cgaaaaatgg tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa
    19201 cgtgggttaa aaggaacagt tgaagatctt acagaaaaag ccaaagacac cttagaagag
    19261 aaattagacg gcacgagagc taaagaattt gctgaagctg tgaagaatcc atccgaagca
    19321 ctagaaaaac aaaaagaaaa agcttcagca gtcaaagaag gactaaaaga tgcgtatggt
    19381 gaaggacaag tcaatgcagc agtgaaggca aatacaacaa gtgaaaaaga aaatcaagaa
    19441 ggtgcagcga ttccttcagc caaagatcac caagatgagt tacattctaa cgaaaattcg
    19501 atcggagaaa aagattcaga agaaggacaa catggtcagt taccaaaaac cgatccagaa
    19561 caatatcgtt ataaatctaa cattaattct gaaaacgaaa aacgcatttc agatttacca
    19621 aaacgaaatc aagaagtaca aacggatgag gattccatcg atctgccaaa tgataatggc
    19681 atcgaagttg gaactgaaaa atcaacgaaa attggtttgc ctaagacaca aaatttacca
    19741 atgaaagata gtcgtttgaa tcaattcaaa gagaatgatc aaagctttgt tcatcaatca
    19801 cctgcaggta ctgttcaaat gaaatcaagt cgtttgcctg aaaatggaaa tctgacaact
    19861 gtttcttaca atttagctga aagtggtgaa acagagaatg cgtccgttca aaatattgtg
    19921 acaaaagaag ctgcacctaa gccaatcaat aaagttcata atggatacag accagcaaac
    19981 gtagcattta ctgcaccgac aaaaataaat gtgacaagag aaactaaaaa caggaacaat
    20041 gaattgaaag aacaactatt gaaggatatc aaaccataaa aggcctattt aagggccttt
    20101 ttctttataa aacaagttta aaatggaaag gagaaaattg tggaggatat tttaatagct
    20161 acgtttataa ctgcactggg agcattttta acgatatttg tggtgactat taaaggattt
    20221 aaagatacgg atagtttaca aaaagatcat cagtctattt tagatggtca aaaaacacat
    20281 accgaaagca ttattgtaca cctttcagaa ggattcactt atttgaaaga tgctatgccc
    20341 acattcacag taaaattaac ggaaatattt cattctgttg agcaaatatc gtcagaattg
    20401 aaagagcaaa gacaacaaaa agatcgtcaa cttggctcac ttaattttag gcaagaaacg
    20461 atacaagaat cattggagaa tgtaaatcga ttaaacgatg aaatgcaacg agttcatttg
    20521 gaaaatgaag aattacgcgc acaaaattat gatttaattc aaaagaaaag aaatctggaa
    20581 caaaaaattg ttgaattaaa agaagaaaat cgtcaattga attatcaatt acgacgagga
    20641 aaaaatcgtt ctcgtgattt aggaaatgaa ttagagttgt aatatttaga aaaacgctgt
    20701 gagaaatcac agtgtttttt a
//
LOCUS       DACEMS010000044        20350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEMS010000044 DACEMS010000000
VERSION     DACEMS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.044"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..189
                     /locus_tag="IW735_001414"
     CDS             <1..189
                     /locus_tag="IW735_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1044424.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="IW735_001415"
     CDS             complement(1055..1585)
                     /locus_tag="IW735_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1044425.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="IW735_001416"
     CDS             complement(1582..2199)
                     /locus_tag="IW735_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1044426.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2781..3002)
                     /locus_tag="IW735_001417"
     CDS             complement(2781..3002)
                     /locus_tag="IW735_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1044427.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3180..4712)
                     /locus_tag="IW735_001418"
     CDS             complement(3180..4712)
                     /locus_tag="IW735_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1044428.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="IW735_001419"
     CDS             complement(4848..5933)
                     /locus_tag="IW735_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1044429.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGRLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="IW735_001420"
     CDS             complement(5948..6667)
                     /locus_tag="IW735_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1044430.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="IW735_001421"
     CDS             complement(6664..7974)
                     /locus_tag="IW735_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1044431.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="IW735_001422"
     CDS             complement(8001..8591)
                     /locus_tag="IW735_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAQ1044432.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="IW735_001423"
     CDS             complement(8909..9970)
                     /locus_tag="IW735_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1044433.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="IW735_001424"
     CDS             complement(10538..11605)
                     /locus_tag="IW735_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1044434.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="IW735_001425"
     CDS             complement(11625..12104)
                     /locus_tag="IW735_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAQ1044435.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="IW735_001426"
     CDS             complement(12121..12582)
                     /locus_tag="IW735_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1044436.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="IW735_001427"
     CDS             complement(12596..13846)
                     /locus_tag="IW735_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1044437.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="IW735_001428"
     CDS             complement(14224..15675)
                     /locus_tag="IW735_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1044438.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="IW735_001429"
     CDS             complement(15996..16760)
                     /locus_tag="IW735_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1044439.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="IW735_001430"
     CDS             complement(16788..17486)
                     /locus_tag="IW735_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1044440.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="IW735_001431"
     CDS             complement(17498..18277)
                     /locus_tag="IW735_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1044441.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="IW735_001432"
     CDS             complement(18293..19237)
                     /locus_tag="IW735_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1044442.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="IW735_001433"
     CDS             complement(19283..20062)
                     /locus_tag="IW735_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044443.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         6451 a         4049 c         2632 g         7218 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatcttttt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aatcttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttgatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
    17401 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
    17461 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
    17521 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
    17581 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
    19321 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
    19381 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
    19441 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
    19501 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
    19561 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
    19621 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
    19681 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
    19741 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
    19801 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
    19861 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
    19921 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
    19981 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
    20041 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
    20101 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
    20161 attcatagaa atatttataa acaggttttc cctatataat cataccacat acagtttttt
    20221 tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta taattaatcg
    20281 ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca aagaataaaa
    20341 taacaaaaat
//
LOCUS       DACEMS010000045        19988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEMS010000045 DACEMS010000000
VERSION     DACEMS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.045"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            308..1969
                     /locus_tag="IW735_001434"
     CDS             308..1969
                     /locus_tag="IW735_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1044444.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2039..2386)
                     /locus_tag="IW735_001435"
     CDS             complement(2039..2386)
                     /locus_tag="IW735_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1044445.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2470..3411
                     /locus_tag="IW735_001436"
     CDS             2470..3411
                     /locus_tag="IW735_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044446.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3412..4455
                     /locus_tag="IW735_001437"
     CDS             3412..4455
                     /locus_tag="IW735_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044447.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4461..5531
                     /locus_tag="IW735_001438"
     CDS             4461..5531
                     /locus_tag="IW735_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044448.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5521..6474
                     /locus_tag="IW735_001439"
     CDS             5521..6474
                     /locus_tag="IW735_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1044449.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6486..7496
                     /locus_tag="IW735_001440"
     CDS             6486..7496
                     /locus_tag="IW735_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1044450.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7781..8602)
                     /locus_tag="IW735_001441"
     CDS             complement(7781..8602)
                     /locus_tag="IW735_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1044451.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8699..8977)
                     /locus_tag="IW735_001442"
     CDS             complement(8699..8977)
                     /locus_tag="IW735_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044452.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9245..11254
                     /gene="metG"
                     /locus_tag="IW735_001443"
     CDS             9245..11254
                     /gene="metG"
                     /locus_tag="IW735_001443"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1044453.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11733..12320)
                     /locus_tag="IW735_001444"
     CDS             complement(11733..12320)
                     /locus_tag="IW735_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044454.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12495..13817
                     /locus_tag="IW735_001445"
     CDS             12495..13817
                     /locus_tag="IW735_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1044455.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13972..14748
                     /locus_tag="IW735_001446"
     CDS             13972..14748
                     /locus_tag="IW735_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1044456.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14741..15310
                     /gene="rnmV"
                     /locus_tag="IW735_001447"
     CDS             14741..15310
                     /gene="rnmV"
                     /locus_tag="IW735_001447"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1044457.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15303..16187
                     /gene="rsmA"
                     /locus_tag="IW735_001448"
     CDS             15303..16187
                     /gene="rsmA"
                     /locus_tag="IW735_001448"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1044458.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16638..17267
                     /locus_tag="IW735_001449"
     CDS             16638..17267
                     /locus_tag="IW735_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044459.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17391..18500)
                     /gene="ugpC"
                     /locus_tag="IW735_001450"
     CDS             complement(17391..18500)
                     /gene="ugpC"
                     /locus_tag="IW735_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1044460.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18709..19131
                     /gene="mgsA"
                     /locus_tag="IW735_001451"
     CDS             18709..19131
                     /gene="mgsA"
                     /locus_tag="IW735_001451"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1044461.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19454..>19988
                     /locus_tag="IW735_001452"
     CDS             19454..>19988
                     /locus_tag="IW735_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1044462.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         6504 a         3591 c         4078 g         5815 t
ORIGIN      
        1 accgtttatt cagataaggg actgtgacaa gacttttgtc acagtttctt tttgtttatt
       61 cttttttatc tttgtatggc aataatagac aatataacga aaattattta atggcttttg
      121 gaagaccatg gaaaaccttg tacaaagcta ttttttctga aaaattcctt gaaacctcac
      181 gaaaaaatca ttattttatg aaaaagacaa aaaaaagtgt tgatttttta ataattattc
      241 attaaactct taaacaactt cagaaaatat taattattct gaaaattcca ataaaggggt
      301 acgtaaaatg aaaaagaaat ttacatttgg tttggttgcg gtatgcggcc ttgtacttgc
      361 tggatgttat ggcggcagtt cagccacaag cagtagttca gcaaaaagcg gttcagcaga
      421 tggaggaggc gtatttaact tagtcgtgcc tcaagaaatg cctacagctg acttatcagt
      481 tgcaacagat acaatcagtt ttacagcatt gaataacgtt tatgaaggaa tttaccgtct
      541 tgatgaagac agcaaaccac agccggcagg agctagcgag ttggcagaag tcagtgaaga
      601 cggattaact tacaaaatca aattacgaga agacgcaaaa tggtcaaatg gtgatcctgt
      661 tacagcagcg gattacgttt atggctggca acgaacagta gacccagcta cagcttctga
      721 atatgcttac ctgtatgcgc cagttgaaaa tgcagaagac attacagctg gaaagaaaga
      781 taaatcagaa ctagggatca aagcagttgg tgactatgaa ttagaaatca aattgacgaa
      841 acaaacacca tatttccaat atctgcttgc tttcccatca tttttcccac aaaaacaatc
      901 cgtagtggaa gaaaatgggg atgcctatgc atctgcaagt gataaagctg tttataatgg
      961 accattcaca ttagaagatt ttgatggacc aggaacagat acagaatgga cttataagaa
     1021 aaatgatcat tactgggaca aagatacagt aaaactttct gaaatcaaag tcagtgtagt
     1081 aaaagaatct tctactgcat tgaacttatt caaagatggc caagcagatg acgtgatctt
     1141 gtctggtgag ttagcacagc aaaacgcaaa cgatccagct tatacttctg ttaaagaagc
     1201 gcggacaagc tatatcgaat tcaatcaacg tgaaaagaat tcaccattca acaatgtgaa
     1261 cctaagaaaa gctatttctt attctatcga tcgtgaagct ttagtaaaac aagtactagg
     1321 tgacggatca gttgtctcta caggattgat cccatcagat atggtaaaaa gtccaacaac
     1381 gaacgaagac tttgcaaaag aagctggaaa attagtttct tatgacaaag ataaagcaaa
     1441 agaatattgg gaaaaagcga aaaaagaatt gggcgttgat tctttagaat ttgatttgat
     1501 ggcttctgat gatgattcat cgaaaaaagt aatcgaatat gttcaaaact caatccaaga
     1561 aaacttggat ggcgtaacag tcaaaccaac acctgttcca ttctctgtac gtcttgaccg
     1621 ctcaacttcg ggtgattttg acgcagtact aggcggatgg gctgctgact atgcagaccc
     1681 aagcagcttt actgatttgt ttgtcacagg taattcatac aaccgtggtc gctggagcaa
     1741 tgcagaatat gacgaagcag ttaaagcagc ttcatcaaca gacgctggcg atgaacaagc
     1801 ccgttgggat gatttccaaa aagcaatcaa gatcatctct gatgatatgg gtgtcgttcc
     1861 ggtgtaccaa aaagcagaag gtcacctagt gaatgaaaaa atcaaaggta tcgttcacca
     1921 tgcggcaggc gcttcatggg attacaaatg gacatacgtt gaagaataat cgttaatacc
     1981 gtgagcatga gaaaaaacga ggctgcggca aatgtcgcaa cctcgttctc atattcactt
     2041 atcctaacaa agaaaaaagc atgaaaatag cagaaagagc aattagtatt ccggcaatga
     2101 tcagccaaaa gctgtacata ccgtagctgg cagacgagag cgaggaaaat tctggttttt
     2161 tctttcggtt ccttgttcgt gcaagatgga ccgatcgctg aaaatgatcg aagaaacccg
     2221 aagaaaagac ccataaaaaa ccgccgataa tcagaaacgg caatgaccat aagaatagat
     2281 cattagataa ttgaatgagt gtaaaggaat ggttgatgag attttttatt aaaataacca
     2341 gaatcagtag aaaagctact gcgtagggga tagatttttt tttcatttga ttgctccttt
     2401 atttttggtt cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg
     2461 gaaacattag tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc
     2521 acgctttggt taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca
     2581 tatacgaatc aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt
     2641 ttggataaac cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat
     2701 tttggaattt ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc
     2761 ggaccatcct tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt
     2821 ttaggaacga tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta
     2881 gcgatattag gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt
     2941 gcaatcaaat tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta
     3001 ccaacaattg cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa
     3061 atggtggaag tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt
     3121 tgggaaatcg cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta
     3181 ggaccgttag ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt
     3241 cctggtatcg gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg
     3301 gctgtaacga tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg
     3361 tacggtctag ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg
     3421 ataccaaagc gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa
     3481 aaaaataccg aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat
     3541 tcatggcgcc gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc
     3601 atcattttga tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac
     3661 gtggcttata tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga
     3721 aaagcaatgg ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac
     3781 gtaaatttct ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg
     3841 ggaatgagaa tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt
     3901 gtagcttacg ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt
     3961 tttttggaaa tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt
     4021 tttgaaccag gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg
     4081 gcacgtatcg ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga
     4141 ttgacattag gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct
     4201 agtgtcatta ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt
     4261 ttaagcttta tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc
     4321 gaagggtaca agacattcct gtatctgcct tatctattat ggattccggc agtaacttta
     4381 tcggttatca tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct
     4441 aaaatgaaag agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt
     4501 ttgatacgta tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag
     4561 gagaaacatt ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca
     4621 tcatgcgctt gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta
     4681 acgatatcgt tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga
     4741 tgattttcca agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg
     4801 ctgagtccct tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag
     4861 aattgcttaa attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc
     4921 agttctcagg aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg
     4981 aaatcctgat tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt
     5041 tggagctgct gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg
     5101 accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg
     5161 ttgaagttgg aacatcggaa gaaattttct acaacccgca acatccctat acttggggac
     5221 tgcttggttc aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt
     5281 ctccaccgga tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg
     5341 cgatgaaaat cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg
     5401 cagcaacttg gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac
     5461 gtcgctgggc tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg
     5521 atgccaaata aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag
     5581 aatgaagtac atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt
     5641 ttagttggag aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat
     5701 gaacctactg atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa
     5761 aaaggtttga cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg
     5821 ttgaatcctc gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta
     5881 gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg
     5941 aatccaagcc acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt
     6001 ggtattgcac gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct
     6061 gcactagatg tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa
     6121 catcaattga cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac
     6181 cgaatcggtg taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat
     6241 aatcacggtg ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca
     6301 gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta
     6361 cgtcaacttc gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca
     6421 tattacgcaa aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga
     6481 catagatgct aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat
     6541 tgattcgtac ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga
     6601 aaagttggca acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag
     6661 aatacccatt cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg
     6721 atcgggcttt tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa
     6781 aaagtatgtg gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg
     6841 gctacttaaa gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg
     6901 aaggatcgat tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac
     6961 tactttgtga tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct
     7021 atcatccgaa gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc
     7081 accagacaga agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg
     7141 ctcaccatag aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag
     7201 taatggatct ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg
     7261 ggaaattggt caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac
     7321 atgctgattt ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc
     7381 aatggtataa taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg
     7441 taagaaagag ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa
     7501 tgaatgaaac tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt
     7561 tgtttctagg aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca
     7621 atagcagaaa aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt
     7681 tacgaataca tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt
     7741 ttttcttgaa tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct
     7801 tgagctgata gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga
     7861 aatattggag catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta
     7921 agaatttagc aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta
     7981 gaacttgcgg ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt
     8041 gactatggtc aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg
     8101 agatagccac ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc
     8161 agcgtacttc cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt
     8221 agctgactgt gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt
     8281 gctcaatatt ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc
     8341 tttctccagt tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat
     8401 aaggatgccc ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc
     8461 acatcagtac gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt
     8521 gataccagtt aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt
     8581 ctttagaagc aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct
     8641 tattgtagca gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt
     8701 aaatatgaaa aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca
     8761 atactttata ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa
     8821 tgataaaacg caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa
     8881 aataaccata catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag
     8941 caatactaag tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag
     9001 atagatagtc tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga
     9061 tatgaaacaa aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt
     9121 gaacgtagat agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg
     9181 ctttttttat ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag
     9241 ccccgtggca gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa
     9301 acttcatatc ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg
     9361 gttgatgggc tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga
     9421 aacaaaagca gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga
     9481 tgtcaagaaa ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga
     9541 tgattaccat aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat
     9601 ttacttaggt gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac
     9661 acaacttgca gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag
     9721 tggacacgaa gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc
     9781 agaccgtcta ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa
     9841 aaacgaaatg atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac
     9901 aacttttact tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat
     9961 cgatgcatta tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt
    10021 cgaaaaatac tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac
    10081 gatctattgg ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg
    10141 gcatggctgg ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta
    10201 tcctgaaatg cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc
    10261 tgttccgttt gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta
    10321 tgatctggca aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata
    10381 ttgtgatgga ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc
    10441 tacgacggct gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa
    10501 tacagcattg gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac
    10561 agaaccatgg gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat
    10621 ccatttagct gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac
    10681 gccagagaag atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt
    10741 acactttggc gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc
    10801 acgtttagat atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca
    10861 aacaaacgaa gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga
    10921 aaaacaaatc aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa
    10981 ctgtcaaaaa gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag
    11041 ccaagaccgt caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat
    11101 cgggaaaaaa gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag
    11161 tcaaggaatg atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc
    11221 aaaatccatg ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt
    11281 tgtgtcatac cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta
    11341 cttgaagcca atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag
    11401 aagtacgagc ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac
    11461 agaaaaaaag cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt
    11521 ttttattgtc cttacattca taaagtttga atgcaatgac tttattacat ctatatttat
    11581 atctaacagt agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt
    11641 gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg
    11701 atgattgttt tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt
    11761 tttgtcttca aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca
    11821 taccagaaaa tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt
    11881 aacaagatca accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg
    11941 acttcactaa gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc
    12001 atccgccagt tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa
    12061 ataccgatag gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc
    12121 acaggacctt tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt
    12181 gataacgttg caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac
    12241 tgctgttgtt ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga
    12301 taagaacgct taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac
    12361 gaaaaataaa aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa
    12421 aaagaatggt aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa
    12481 gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag
    12541 ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg
    12601 ctagtactca gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga
    12661 tctgtgctgg attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg
    12721 taaaatatat tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa
    12781 gcttgattta ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga
    12841 cgaacttatt acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt
    12901 ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt
    12961 tgattgttaa attaatacca attgctgtga ttgtcatatg gggattattg accccaggac
    13021 aaggaactgt ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat
    13081 tgagcagtgc gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa
    13141 tggcagggga aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga
    13201 gttttgtaac agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg
    13261 atcatcttgc cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa
    13321 tcggtggaaa gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt
    13381 atacactaac tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca
    13441 gtaaacagct gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc
    13501 agttagcagt agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt
    13561 tgatttttgt gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa
    13621 aaaaagcgcc ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta
    13681 ttgcgattct cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag
    13741 cattgatcgg tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa
    13801 aacgctcaga agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct
    13861 agatcttcgt gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact
    13921 tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt
    13981 gattctcata cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag
    14041 cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt
    14101 gaaaagtctt tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat
    14161 ccgacagagg ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact
    14221 ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg
    14281 aaagaagtcc aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta
    14341 ccaatcagta ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa
    14401 gatgttcgag atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa
    14461 cgatttcttg acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca
    14521 ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat
    14581 gccccttatc tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt
    14641 tatacagtag aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa
    14701 acatggacaa atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca
    14761 agagattata gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc
    14821 agatacgata gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca
    14881 tgcccaagag actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat
    14941 aagaaaaatc attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca
    15001 agctaaacca aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt
    15061 agaagcattg aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac
    15121 tagacaagac cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga
    15181 aaaactaggt gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt
    15241 gaagatgttt cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca
    15301 acatgagtga atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac
    15361 atggtttttc atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa
    15421 ggaaaatcgt agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag
    15481 gaatcggtgc attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga
    15541 tcgatgatcg tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta
    15601 ttgtacatca agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg
    15661 aggagcttcc gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga
    15721 tgcattttct tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag
    15781 tagctgatcg tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg
    15841 ttcaatatta tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc
    15901 agccaaatgt agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag
    15961 tgacagacga aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa
    16021 cgctttggaa taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag
    16081 caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac
    16141 aagaattcgc cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt
    16201 gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    16261 ttcctcatgt ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16321 ttaagaaaga ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa
    16381 acagcttctc atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact
    16441 attatgccga acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg
    16501 atcaaaaaat tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat
    16561 gtgtcttgac agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat
    16621 attaggagag agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat
    16681 tgattgggat gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt
    16741 cctttacttt ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg
    16801 cagtggctgt tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg
    16861 cattgcgagc attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc
    16921 atccgagtat cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt
    16981 tcaattttgt gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt
    17041 actttattgg taatatgcct gaaacatatt attctcaagg atatttttat acagtattca
    17101 tcttgatggg gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta
    17161 tagcaggaac actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa
    17221 atcggaaaga gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca
    17281 aaaaaaaaac tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc
    17341 taaaaagagc tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt
    17401 ttgatgactt gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg
    17461 tcgactattt cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta
    17521 gaaccagttt ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct
    17581 gatttcacta cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct
    17641 ggacgaatac cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt
    17701 tttccttcag gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat
    17761 gtcacattaa agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg
    17821 ggtgtatcat aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata
    17881 acgattcggt cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt
    17941 gtttcaagac gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca
    18001 tctaaatttg ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt
    18061 cccaatgcca cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat
    18121 tctgtcagac ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca
    18181 tatttacgta atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat
    18241 gcgtagtttt ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact
    18301 tttccgccga tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa
    18361 gtagttgact ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt
    18421 gatagattaa aatcagttac agaataattt tctgcattgt catatttttt atatacgttt
    18481 ttcagagcca tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt
    18541 taaagtataa atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt
    18601 ttttttgtca atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat
    18661 acactggagt aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct
    18721 ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca
    18781 atattggaaa aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca
    18841 accggcttgc cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga
    18901 gccatgattt cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca
    18961 cagccccatg aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg
    19021 ttagctacga atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta
    19081 gattttcgaa gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc
    19141 gtatggaaaa ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga
    19201 taatatgcga tataatacga acgtattgga taatataaac aataatatta gtttttcgta
    19261 tttgcagtaa atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac
    19321 agagtagaaa ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga
    19381 cgtaatgttc gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa
    19441 ggagatgcta aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt
    19501 attgattgca ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa
    19561 aatcagtgtc acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc
    19621 agggataggc gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt
    19681 ctttattgga tttacattga atgcatttat tgaaggagca atctacggat tctttttcta
    19741 tcgaaaagag atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat
    19801 caacttattt ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg
    19861 ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc
    19921 catttattac gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca
    19981 cgctaaat
//
LOCUS       DACEMS010000046        19474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEMS010000046 DACEMS010000000
VERSION     DACEMS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.046"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..3654
                     /locus_tag="IW735_001453"
     CDS             127..3654
                     /locus_tag="IW735_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1044463.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IW735_001454"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IW735_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1044464.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IW735_001455"
     CDS             7442..7783
                     /locus_tag="IW735_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1044465.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IW735_001456"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IW735_001456"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1044466.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IW735_001457"
     CDS             9140..11560
                     /locus_tag="IW735_001457"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1044467.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW735_001458"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW735_001458"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1044468.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="IW735_001459"
     CDS             12994..13731
                     /locus_tag="IW735_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044469.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="IW735_001460"
     CDS             13745..14560
                     /locus_tag="IW735_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1044470.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="IW735_001461"
     CDS             14573..15220
                     /locus_tag="IW735_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1044471.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="IW735_001462"
     CDS             15236..15892
                     /locus_tag="IW735_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1044472.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="IW735_001463"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="IW735_001463"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1044473.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="IW735_001464"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="IW735_001464"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1044474.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="IW735_001465"
     CDS             18234..18752
                     /locus_tag="IW735_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1044475.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="IW735_001466"
     CDS             18859..19359
                     /locus_tag="IW735_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1044476.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6553 a         3344 c         4180 g         5397 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aagaaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaag
//
LOCUS       DACEMS010000047        18952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEMS010000047 DACEMS010000000
VERSION     DACEMS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.047"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(146..1111)
                     /locus_tag="IW735_001467"
     CDS             complement(146..1111)
                     /locus_tag="IW735_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1044477.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="IW735_001468"
     CDS             1345..2190
                     /locus_tag="IW735_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1044478.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="IW735_001469"
     CDS             2381..3568
                     /locus_tag="IW735_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1044479.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            3574..3930
                     /locus_tag="IW735_001470"
     CDS             3574..3930
                     /locus_tag="IW735_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1044480.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="IW735_001471"
     CDS             3932..4270
                     /locus_tag="IW735_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1044481.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            4665..5699
                     /locus_tag="IW735_001472"
     CDS             4665..5699
                     /locus_tag="IW735_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1044482.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="IW735_001473"
     CDS             5692..6378
                     /locus_tag="IW735_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044483.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="IW735_001474"
     CDS             6402..7250
                     /locus_tag="IW735_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1044484.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="IW735_001475"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="IW735_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1044485.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="IW735_001476"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="IW735_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1044486.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="IW735_001477"
     CDS             9516..10751
                     /locus_tag="IW735_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1044487.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /locus_tag="IW735_001478"
     CDS             10738..11208
                     /locus_tag="IW735_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1044488.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="IW735_001479"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="IW735_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1044489.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12699..13841)
                     /locus_tag="IW735_001480"
     CDS             complement(12699..13841)
                     /locus_tag="IW735_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1044490.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13880..14614)
                     /locus_tag="IW735_001481"
     CDS             complement(13880..14614)
                     /locus_tag="IW735_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1044491.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            14846..15121
                     /locus_tag="IW735_001482"
     CDS             14846..15121
                     /locus_tag="IW735_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1044492.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            15219..17216
                     /locus_tag="IW735_001483"
     CDS             15219..17216
                     /locus_tag="IW735_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044493.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(17292..17627)
                     /locus_tag="IW735_001484"
     CDS             complement(17292..17627)
                     /locus_tag="IW735_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1044494.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            17754..17969
                     /locus_tag="IW735_001485"
     CDS             17754..17969
                     /locus_tag="IW735_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1044495.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            18006..18368
                     /locus_tag="IW735_001486"
     CDS             18006..18368
                     /locus_tag="IW735_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1044496.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            18414..18740
                     /locus_tag="IW735_001487"
     CDS             18414..18740
                     /locus_tag="IW735_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044497.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         6292 a         3267 c         3950 g         5443 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 gatgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgta agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa tcataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atatataagg gttctagctt ttttgttttg
    12661 cctgtatact agaaatgacc agattttgac cagctctttt actgatgagc catcatatca
    12721 gcaaagtctt gacctgtttt cttaggtgca ttatttgtaa tatgtgcata gatgtctaat
    12781 gtcactttac tattttcatg ccctaatcgt tgttgaacta ccttgacaga ttctcctgct
    12841 tctaataata aactagcatg tgtatgtcta aacccatgag gtgtaatacg aggcaagtta
    12901 tattttttca aaatatatct taaccaatca ttaacagtgt ttggaatgta tagagtgttt
    12961 ttaactgttg taaaaacatg ttgctcttta gagctagtat tgtatccaag ttttaaatac
    13021 tcttcttttt gctgtaattt ccagttgttg aggatagaaa gagtctcagt atctaaagag
    13081 ataactcgtc tagaagctct tgttttcgga gattgaatta taactttacc aaattcatct
    13141 acagcaagag ttttattgac atgtagttct ttattaaacg tgtctatgtc tttccactgt
    13201 aaagcgagta cttcactctt tctcatacct gtgtatgcca ataatctgaa gaaggcgtat
    13261 tgtttcatgt taccaaaatc tttaaaagca tccagtaaat tattaagctc ctcttttgag
    13321 taaaatttac gatttttctc atactctttt tttcgtggaa gaagtgtttt ttccatagga
    13381 ttattgtcaa tatattgcat tgcaaccccg tagcgtaaaa tttgtgcagt ctgttttctc
    13441 atataatcgt aactttcata tttactgtgc cattcatcta ccactttttg acaatatggg
    13501 acagagatat tcgatagttt aagcttacca aatgctggaa gtatttgatt atttgcgtat
    13561 cttactgaga ttgcgattgt tgaagcttta acactggtac gctgatgagt tatccattca
    13621 ttatacaggt cttcaaatgt catatcaggt gctttctttt taacaccgta taatatttga
    13681 tgtctgtact cgtcataatc tttttgagct tcttgtttgc tactaaaacc tcttctcgta
    13741 gtatttattt tttcaccagt tcgtggatcg gtggcaatgt gtcctttata catccacgcc
    13801 acttgaccgt ttttcttttt gtactttgat atttttgcca ttaaatcatc tcctcttagt
    13861 ttacaaggag tacttaccac tagatggtat cacctccctt catttttttc ttagtgtatt
    13921 ttttcccaat tgatgaaatc gtttgttcta gcttgtttat attatcagta atttctctca
    13981 cagcttcttc gggataagac atgtctttca attgaatagg tagccattca tctaccattc
    14041 tttctagtag ttcttctata ctgctagtga ggaaaagaat gttattgtca aattcttcta
    14101 caatagcatt gatgaataat tcttctattt ttaagatatt gaaaagacta attttttcat
    14161 ttttaacttt attacaagtc ttgttagcaa taaattcttg catctctggt tctaatttgg
    14221 aatatctatc cagccatgta tctaaattag agtgtactgg tgggaactgt tgacgaagat
    14281 gctggaacag gtttttatct ggaaatgtta taccatctag tacaatgcta tctctataaa
    14341 gaataattcc caaaatatat tcttctacat tgccacaaac taactcctct atactcatat
    14401 ttcccaaatc agcaattcgg gataatcgct ctggtattgg taaataggtt cccttctccc
    14461 attcgtagat agtttttttg tcaattaatt gaccgttgtt aatcataaga ccaaatgctg
    14521 gttgtgttaa ttttttactc tttcttatag cttttatttt tgttccgagt atcttttttc
    14581 gttctaaata atttattggt ggacgtttta tcatattgac cacacctcct aaaattagta
    14641 tacctcaaaa aaggggtgtt gtaaataaat tttacaaagc tgttgacacc ccttttttat
    14701 cgaggtatta tgtgtatgtc gatgaactgg taatcatttt ttgtttgatt aatcggttaa
    14761 taacaaaata cttttatttt gagcaaaaaa tacgaagata attcttagaa aatttgttca
    14821 agcaatcacg aaaggagggg gaaatatgtc tctacaaatc attgatagca ttgtttctat
    14881 acaagttgaa caacaagtga aagagataat tacagtgcta aagcctatgc tagaagacat
    14941 tatttctgga gtcactctag agtatatgga ctatcagcag gcaagtgaat atctaggtgt
    15001 atcagtagga acgattagaa aatatgtttc tcaatacggg ttacctgtta taaaaattga
    15061 tacggtcatt agatttaaaa aatctgatat tgatagtttc atggaacggt ataaaggttg
    15121 agtaaacaaa taagactaaa acatatagta ataaagagtt tgtttttatt atctgtatgt
    15181 tttagtcttt aacagaattt tataaggagg aaacacaaat ggaaacatca atgattaata
    15241 ttccaattta tgtagatcaa gaaaaattat tagagaagcc agttacaaaa aaagaaaaaa
    15301 ctgatgtaag agataggtgt ttagataact atagagaagt tacaagagat gagtttgtag
    15361 agatactaaa ttcatcacaa tcatttattc caagcaagac aaagaataaa ggcaatgaca
    15421 aggagagttt tgtggaaacc agagtaatta ttctggatgt cgataacact gttaaagatg
    15481 agaaggggaa ggtagtagat ttgagtaaag atgatagtag atacttaagc atagaaaagg
    15541 ttttatctat caatttagtt cataattctg cgtttgcgat acaaaaaagt attcggtact
    15601 ctgaaaatct agaaaaatac aagattgtct ttctattaaa agaagcaatt actgactatg
    15661 gtaaaatggt tgctgtttac gaatacttga aaaagaacat ccccgggtgt gatgacaatg
    15721 taaatgcttc gaatagaatg ttttttggag gatataagtc agatgcagtg ataataatta
    15781 atgaagacaa catgttagat attactgagc ttccgattga ttttaccatg acatgttcta
    15841 aaaattgtca aagtgattca gggagagagt atgaactagt caatgaaacc gattttgtta
    15901 agttgataaa aaatgacgac aaagaagaaa tgaaacagtg gtttaaagac tgcctgtttg
    15961 attcctccga catgtctttt aatgagattt atgaaagatt gttaaccatt gatatgaata
    16021 aattacttaa gtcaagtaaa aacttaagat gtctgtttca caacgatcat aatccctctg
    16081 ctagtatttt taccagtaaa actgggcatt ccatttttta ttgtcattcc agtaattgtg
    16141 atatcagtga aaattttatt ggagttgtga tgctaatttt aaagaaagac agtcgggtag
    16201 aaacatttaa ttggctatta gagaacctag acttgtatcc tacacatttt cgaaaattga
    16261 aagaggaatc aaaagtactt tttgacactc ttgaatcaat gaaagacaaa cttttcagga
    16321 caataaggaa ttatctagag gaaatgcgac gtgtctatga tgtcttgctt tctgatgtta
    16381 atttttttga tgatagtaag gaaagtgtga cttgtatttt gtcgggggaa caattagcta
    16441 aaaaaatgag ttcttactat ggtaaaactt atgacataga taaatgtaat aagttactat
    16501 cacttatgac ttttatagga ttgataaaaa aattagatga tgaagatgtt cctttgaaga
    16561 tgctcgaata cctaaatagg ttaaaggaag agaaagcgtt aggaaacttt aatcataaga
    16621 gaagcaatgt ctatcgatta gacgttttta atgcaacttc tgtgattcaa aaattgaatg
    16681 aagatatcct tccacttctg gaaaaaattc attttacata tcaatatttt tcttatgaat
    16741 gggttaagat atgttttaac gaaaaagagg ctaaaagagt atttcctcaa aattcccgat
    16801 cagaactcag caaggaaaaa caattgattt tagatgaagc acatgaagtt ttacagggct
    16861 tatcgtggca agggacatat ctaatgacag agaaagaact tattaacact gtttgtgaac
    16921 gcctacgtaa tgttggggat aacagcgtga aaaatgcttt aataaaaaac agaggctact
    16981 ttgttaaaca aggattcact cttgaacgag cttctaagga tattaagaaa ctatacaatg
    17041 tgaaagcaag tagtagtttt ttaatatatt tagcgaaagg ggcagggata gatgaaacaa
    17101 taaaagaatt gatattatca ggagctggga tagttcctct gtatcaattt acaagtgtaa
    17161 caagagatga tggacggaga gtaaggaaga gaaaagaccc aattgtatta aactagacag
    17221 gttatccata gctcttaatc ttatttcatc tcaatacacc caccgttaaa gttcggtggg
    17281 ttatttattt ttcaatcttt caatagctga ttgcgttcat tttcttgcct ttttaatagc
    17341 ttcatatagt caattgttcg aactgcatct tcagtgctaa gagaacgata gagagccaaa
    17401 aactcttcct cataatctac gacaacattg tctagaaaat ggcttacagg ggttcctagg
    17461 gctctagaaa gagctgttaa gctatcgagg ttaggctctg ttacagaatt ttcccatctt
    17521 gctactgcct gttgtgtaga gaagattttt tcagctaatt ctttttgtgt ccacccgaga
    17581 gcttttcgct tttctctaat gacctttgca tattgtttct gtttcattaa tacaccccca
    17641 tctcagtata gtacaacaag ttgttgtatt tgtaatggta tttgaagttt tttatatttt
    17701 atgagttgaa atacatcata ttgttgtata taataaaaca ggaggggaga atcatgaaaa
    17761 aagtaagtaa tctcagccga tacagaaaag aaaaaggact ttctcaaacg gagttagcaa
    17821 aaaaaatgaa tgttacacag caatgtgtaa gttcgtggca aacagggaga acgattccta
    17881 aaccctatca aatgaagatg ctttctgaga tattaagtgt accaatgaat gagctgtttt
    17941 ctgatgtatt taataaagta aacagttaac taataagcaa ttgaatgatt ttaggaggag
    18001 aagacatgta ttgtagaaaa tgtggcaaag aaataacaga tgaaagtaag tattgccagt
    18061 tttgtggagc actacaagaa acagatgaga caaccggaac agacacagta aagaaaagta
    18121 ctgagcagac aattaatgaa atgaaggaac aaatcgtaca tggacaagag acagggaagc
    18181 tgtatttgat tattggttgg atttctatgg tagtatcgtt agcatttatt cctgttgtat
    18241 ttggtgctgt tgctgttatt atgggctact tatatcgaga aaaagatgag aaaatgggta
    18301 cgattttgat gatagcaggg gtagctggag caatctttgg tgtactttta gggatgtcaa
    18361 tgtactgata agagggataa gaacaaatta ttttggagat aggagataaa aagttgaata
    18421 aagtaaatat gaacaagaag tttgtcatta taggtattgt tgtcttggct attataggta
    18481 ttattttttc aaaaatgaca ggtacatctg gattagagaa gtcatcaaaa ccattggtaa
    18541 cacagatttt acaagaacag tacaacttaa atcgatcatg tgatgatgtg acgattactc
    18601 atgaaaatgg agataacaca taccgggcta aagctatatt agacaatgga agtgctatca
    18661 acataaatat tgagtactat ccaaagaaag accgtatata tgtggagata ccatatgctg
    18721 aggtgttaat gttaaattga tttaaatgta ttatgcatac cgtccaaaca ggaactccca
    18781 aaggtgtaaa ttcactaggg agtttttata gtttcctata aattaaatac taatacaggt
    18841 gacaaaaaaa tattacaatg attgtcggga atttttatgt tattcactgt tataacaata
    18901 taaaagaaat gaaaaagaaa aaattattat ggatatgttt ggtattttta tt
//
LOCUS       DACEMS010000048        18829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEMS010000048 DACEMS010000000
VERSION     DACEMS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.048"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            21..1448
                     /locus_tag="IW735_001488"
     CDS             21..1448
                     /locus_tag="IW735_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1044498.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="IW735_001489"
     CDS             complement(1540..1905)
                     /locus_tag="IW735_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1044499.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="IW735_001490"
     CDS             complement(1902..2267)
                     /locus_tag="IW735_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1044500.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="IW735_001491"
     CDS             complement(2459..2707)
                     /locus_tag="IW735_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044501.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="IW735_001492"
     CDS             2706..2834
                     /locus_tag="IW735_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1044502.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="IW735_001493"
     CDS             2891..4585
                     /locus_tag="IW735_001493"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1044503.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4589..4708
                     /locus_tag="IW735_001494"
                     /pseudo
     CDS             4589..4708
                     /locus_tag="IW735_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4790..4981
                     /locus_tag="IW735_001495"
     CDS             4790..4981
                     /locus_tag="IW735_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044504.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            5359..6492
                     /locus_tag="IW735_001496"
     CDS             5359..6492
                     /locus_tag="IW735_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1044505.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6523..6843
                     /locus_tag="IW735_001497"
     CDS             6523..6843
                     /locus_tag="IW735_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044506.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6872..7336)
                     /locus_tag="IW735_001498"
     CDS             complement(6872..7336)
                     /locus_tag="IW735_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1044507.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7467..8724)
                     /locus_tag="IW735_001499"
                     /pseudo
     CDS             complement(7467..8724)
                     /locus_tag="IW735_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8753..9070)
                     /locus_tag="IW735_001500"
     CDS             complement(8753..9070)
                     /locus_tag="IW735_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1044508.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9096..9416)
                     /locus_tag="IW735_001501"
     CDS             complement(9096..9416)
                     /locus_tag="IW735_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1044509.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9880..11295)
                     /locus_tag="IW735_001502"
     CDS             complement(9880..11295)
                     /locus_tag="IW735_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1044510.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11312..12628)
                     /locus_tag="IW735_001503"
     CDS             complement(11312..12628)
                     /locus_tag="IW735_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1044511.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12778..13476)
                     /locus_tag="IW735_001504"
     CDS             complement(12778..13476)
                     /locus_tag="IW735_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ1044512.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13765..14754)
                     /locus_tag="IW735_001505"
     CDS             complement(13765..14754)
                     /locus_tag="IW735_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ1044513.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14834..15667)
                     /gene="msrA"
                     /locus_tag="IW735_001506"
     CDS             complement(14834..15667)
                     /gene="msrA"
                     /locus_tag="IW735_001506"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1044514.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15842..17476)
                     /locus_tag="IW735_001507"
     CDS             complement(15842..17476)
                     /locus_tag="IW735_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044515.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17463..18797)
                     /locus_tag="IW735_001508"
     CDS             complement(17463..18797)
                     /locus_tag="IW735_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044516.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         5942 a         3724 c         3156 g         6007 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
     4741 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
     4801 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
     4861 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
     4921 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
     4981 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
     5041 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
     5101 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
     5161 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
     5221 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
     5281 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
     5341 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
     5401 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
     5461 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
     5521 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
     5581 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
     5641 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
     5701 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
     5761 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
     5821 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
     5881 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
     5941 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
     6001 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
     6061 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
     6121 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
     6181 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
     6241 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
     6301 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
     6361 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
     6421 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
     6481 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
     6541 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
     6601 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
     6661 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
     6721 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
     6781 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
     6841 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
     6901 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
     6961 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
     7021 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
     7081 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
     7141 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
     7201 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
     7261 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
     7321 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
     7381 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
     7441 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
     7501 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
     7561 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
     7621 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
     7681 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
     7741 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
     7801 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
     7861 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
     7921 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
     7981 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
     8041 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
     8101 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
     8161 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
     8221 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
     8281 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
     8341 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
     8401 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gcaagaaagc
     8461 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
     8521 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
     8581 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
     8641 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
     8701 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
     8761 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
     8821 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
     8881 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
     8941 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
     9001 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
     9061 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
     9121 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
     9181 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
     9241 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
     9301 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
     9361 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
     9421 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
     9481 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
     9541 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
     9601 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
     9661 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
     9721 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
     9781 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
     9841 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
     9901 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
     9961 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    10021 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    10081 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    10141 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    10201 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    10261 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    10321 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    10381 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    10441 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    10501 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    10561 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    10621 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    10681 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    10741 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    10801 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    10861 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    10921 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    10981 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    11041 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    11101 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    11161 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    11221 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    11281 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    11341 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    11401 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    11461 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    11521 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    11581 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    11641 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    11701 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    11761 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    11821 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    11881 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    11941 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    12001 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    12061 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    12121 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    12181 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    12241 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    12301 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    12361 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    12421 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    12481 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    12541 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    12601 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    12661 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    12721 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    12781 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    12841 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    12901 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    12961 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    13021 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    13081 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    13141 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    13201 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    13261 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    13321 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    13381 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    13441 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    13501 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    13561 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    13621 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    13681 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    13741 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    13801 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    13861 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    13921 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    13981 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    14041 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    14101 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    14161 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    14221 agtcattttt tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc
    14281 ttgtcttaca agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg
    14341 gcgatgatga tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca
    14401 acttgaaatg atggatttac gagagcccca ttccccatat ggcccttccc acatatgata
    14461 acctgctttg ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat
    14521 acgcccaaag tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa
    14581 atgggtaatt cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc
    14641 ataatcacca aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg
    14701 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    14761 tcctcctcat caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt
    14821 taaagaaaag gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta
    14881 ctcgttgata gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt
    14941 gttttttgta aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga
    15001 tttttgtgac gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt
    15061 ccgctaattc tttttgtcgt tcgccttgat aaaaaatcac tggacgatat tgcttccctc
    15121 tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca
    15181 actcgctata acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg
    15241 tgtaaccacc acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg
    15301 acaaaacaga taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc
    15361 cccctgcaaa tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt
    15421 catatttttg ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag
    15481 gcgtcagatt cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt
    15541 ctttaacgga tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc
    15601 aatctcgtgt attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct
    15661 catccataga ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat
    15721 cataaagtga ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga
    15781 gagaatcctt atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa
    15841 tttacctttt atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa
    15901 gataagaaat aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt
    15961 tgactccgct tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat
    16021 ttatttctac attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct
    16081 cattagttac tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt
    16141 gactgccaat ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag
    16201 gagaataatc atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt
    16261 tgtttacaac aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag
    16321 aaacagttaa agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct
    16381 tattgtatct tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt
    16441 gcaagcttaa gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat
    16501 accatggcac caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt
    16561 tttctatgtt tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa
    16621 ctagtcccat tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca
    16681 cattagatgc atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca
    16741 tttgttcaaa catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac
    16801 taaataattt tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac
    16861 gaataaaaat aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt
    16921 acactagcaa actcatactc aatgctagct ttacagacgg atacttttct tcttttccag
    16981 agatgatttg ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg
    17041 caattgcgga acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac
    17101 taaataaaaa tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga
    17161 gtgttgtaca caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg
    17221 tcatccaata taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac
    17281 catatccagt ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc
    17341 ccttgattcg agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt
    17401 tgaaaaagat gaaagaatat atactactaa ctataattaa tccactcaat attactactt
    17461 ttttatttat tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc
    17521 aacttattag gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata
    17581 ctgatccata aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt
    17641 tctccagcat ttacatccac attcactaag ccatgttttt tataagagtc atttacgact
    17701 ttaccgttta cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc
    17761 tttttgttgc ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt
    17821 tcccacgatt ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct
    17881 tctgttgggg cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt
    17941 tcttcattga taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt
    18001 gctgctatag acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat
    18061 tttttattca tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt
    18121 tggataccta agatacgcca aggaaactgg agcaagctta tcggtgtttt ttgaaacaaa
    18181 aaccatggaa ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt
    18241 ttttcttttt tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct
    18301 aataacccca tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa
    18361 atagaaagtg cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc
    18421 atctgctgca acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc
    18481 tttactaaat tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa
    18541 cgaactataa tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta
    18601 tacacaagta gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc
    18661 aatccataag cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt
    18721 gcggatcgat aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat
    18781 aaaaaagcag catctatctt tttaaaaaac ttttctccat aataaaaac
//
LOCUS       DACEMS010000049        18339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEMS010000049 DACEMS010000000
VERSION     DACEMS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.049"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(131..697)
                     /locus_tag="IW735_001509"
     CDS             complement(131..697)
                     /locus_tag="IW735_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1044517.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(697..1653)
                     /locus_tag="IW735_001510"
     CDS             complement(697..1653)
                     /locus_tag="IW735_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1044518.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1653..2309)
                     /locus_tag="IW735_001511"
     CDS             complement(1653..2309)
                     /locus_tag="IW735_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1044519.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2679..4697
                     /locus_tag="IW735_001512"
     CDS             2679..4697
                     /locus_tag="IW735_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1044520.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5124..7538
                     /locus_tag="IW735_001513"
     CDS             5124..7538
                     /locus_tag="IW735_001513"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1044521.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7886..9439
                     /locus_tag="IW735_001514"
     CDS             7886..9439
                     /locus_tag="IW735_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1044522.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9820..11604)
                     /locus_tag="IW735_001515"
     CDS             complement(9820..11604)
                     /locus_tag="IW735_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044523.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11619..13340)
                     /locus_tag="IW735_001516"
     CDS             complement(11619..13340)
                     /locus_tag="IW735_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044524.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13440..14201
                     /locus_tag="IW735_001517"
     CDS             13440..14201
                     /locus_tag="IW735_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1044525.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15168..15436
                     /locus_tag="IW735_001518"
                     /pseudo
     CDS             15168..15436
                     /locus_tag="IW735_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16108..>18339
                     /locus_tag="IW735_001519"
     CDS             16108..>18339
                     /locus_tag="IW735_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044526.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6017 a         3270 c         3713 g         5339 t
ORIGIN      
        1 ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga
       61 aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg
      121 cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt
      181 tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat
      241 caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc
      301 gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca
      361 tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga
      421 gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga
      481 cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct
      541 ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat
      601 cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca
      661 gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac
      721 atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac
      781 ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt
      841 caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac
      901 atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt
      961 tttgcgcatc agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt
     1021 gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg
     1081 cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata
     1141 atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc
     1201 tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag
     1261 acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc
     1321 aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac
     1381 gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg
     1441 gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa
     1501 agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat
     1561 cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata
     1621 tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta
     1681 tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg
     1741 agtaagtagc cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc
     1801 ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt
     1861 tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca
     1921 tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct
     1981 cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat aaatccagaa
     2041 acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca
     2101 aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc
     2161 agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac
     2221 gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca
     2281 gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta
     2341 tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct
     2401 ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa
     2461 tgcaaatgaa atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac
     2521 gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat
     2581 gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa
     2641 ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag
     2701 aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg
     2761 tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc
     2821 aagcggatga aacacctgtt cagttcggcg caagaatcaa tacagcggca tttatcacag
     2881 aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga
     2941 tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt
     3001 ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg
     3061 aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg
     3121 ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt
     3181 actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga
     3241 cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt
     3301 acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag
     3361 gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa
     3421 actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa
     3481 tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca
     3541 atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga
     3601 cgaataattc cggtaacaat accaactctt caggaaatgc tggtacatca aacggaggac
     3661 aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg
     3721 atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt
     3781 atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg
     3841 gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag
     3901 cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat
     3961 acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc
     4021 caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag
     4081 gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag
     4141 gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca
     4201 cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc
     4261 aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag
     4321 cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga
     4381 tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga
     4441 tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag
     4501 tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa
     4561 cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa
     4621 tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt
     4681 tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat
     4741 ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg
     4801 aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg
     4861 agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga
     4921 ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc
     4981 aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga
     5041 aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt
     5101 tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc
     5161 aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat
     5221 tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg
     5281 aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc
     5341 tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag
     5401 gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt
     5461 ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata
     5521 aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag
     5581 tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg
     5641 ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga
     5701 aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa
     5761 gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta
     5821 aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat actctattcg
     5881 gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg
     5941 aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga
     6001 gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag
     6061 ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag
     6121 gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg
     6181 gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag
     6241 atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata
     6301 aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac
     6361 gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg
     6421 aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa
     6481 aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca
     6541 atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat
     6601 gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag
     6661 cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac
     6721 atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg
     6781 ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa
     6841 acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa
     6901 cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga
     6961 ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt
     7021 tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc
     7081 ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt
     7141 tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc
     7201 tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg
     7261 gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa
     7321 catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta
     7381 aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag
     7441 cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg
     7501 cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac
     7561 tgatgagttg tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt
     7621 tgttatttca aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac
     7681 acgttcaatg tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt
     7741 ttgtctatta atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc
     7801 aaattcataa attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa
     7861 tgaagtatga gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc
     7921 agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct
     7981 tttaagctta gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg
     8041 ccatatgagt gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca
     8101 cttttcgtgg ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat
     8161 ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca
     8221 acagttttta cataataaac caacttctca agatgatgac tatctttatt ttgagaagac
     8281 ttcgacgagt caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact
     8341 tgatgattta cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca
     8401 tattttttta ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata
     8461 cctgacaact aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac
     8521 aatctattat gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca
     8581 catgacaatg aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc
     8641 tgatcctaag caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac
     8701 agagacgcac tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat
     8761 tgattcgcaa tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg
     8821 ttattggatg gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc
     8881 tggggatgat gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat
     8941 tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg
     9001 tctatttatc tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac
     9061 tatggatggc ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca
     9121 aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta
     9181 tacaaacaac ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca
     9241 gacagggggg atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac
     9301 gttagagaca aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc
     9361 ctgcctcttc cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca
     9421 agtagaacaa ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca
     9481 agaaacaaat tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga
     9541 gttgacaaga gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt
     9601 gtcttattgc cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca
     9661 agtcttttgt cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt
     9721 attattcttt gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga
     9781 ctaatcatac atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact
     9841 attataaaga ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc
     9901 gacgatatcg ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg
     9961 gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa
    10021 tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt
    10081 cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt
    10141 ttcgctagaa atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg
    10201 tgcgatttca gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat
    10261 attatcagct accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg
    10321 acgtagttct ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt
    10381 attcagttca taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg
    10441 accaacgatc gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat
    10501 tttatctgat gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc
    10561 taaaagtctt gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat
    10621 cctatcgatt gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat
    10681 tggttcagaa atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc
    10741 acctgataga acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat
    10801 gaaacgaatg gccggataga tagcaaagtt cataaattga gcacgtttaa aagcgttgta
    10861 ttgcttttta tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt
    10921 gaccactaga ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata
    10981 ctgattctgt tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact
    11041 gccaacgatc agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag
    11101 accagcagca gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac
    11161 ttgagaaagt tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt
    11221 gtcaaaaaac gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa
    11281 tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct
    11341 taagactgaa cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact
    11401 gaaagaaagc tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat
    11461 tcccatgatc aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa
    11521 tccgtaaaaa tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt
    11581 gttcaacgtt tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca
    11641 aactcctgat aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt
    11701 tttccttcat ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca
    11761 acaatcacga gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct
    11821 gttttatagt ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc
    11881 aacgctcgag caatacatat ccgctgtttc tggccccctg aataattaga accgccttga
    11941 gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc
    12001 ttgatcgctt tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt
    12061 aaaattgtcc cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc
    12121 tgttcttgag tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg
    12181 tcactaagtc tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg
    12241 atcgctgttg tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag
    12301 atttcagtat atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt
    12361 ggttcagcta ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct
    12421 tctatccgtc gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc
    12481 atcaagtaag caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt
    12541 aatgctcctt ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc
    12601 atgagcaaaa aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga
    12661 ttagcatagt cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag
    12721 gcacggatca tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt
    12781 tttggctgga tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat
    12841 acataaaaaa caacagagat caatgtaacc catgttagta atagagaata acgaaacatc
    12901 atgagaatgg tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca
    12961 atcatttgct ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta
    13021 aaacgactga tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt
    13081 tggaatccaa acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt
    13141 gttcctagaa acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg
    13201 ccacttgcta tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg
    13261 gcgatttgta aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag
    13321 aatgttttta agatagacat attattcctc cttttttcct gatacttttc gattctatac
    13381 cttcaagtaa cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa
    13441 tgaaaaaaat gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga
    13501 tccgctattg ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc
    13561 gattgtttga tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag
    13621 atataccgat caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga
    13681 cacttgatga aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa
    13741 aagagataag ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat
    13801 ttcctgtaga agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg
    13861 aaatcaaaaa atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca
    13921 gttcacagac gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag
    13981 acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga
    14041 tgaaccatgc ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat
    14101 ggaaaacagg agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca
    14161 gaactgaata ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata
    14221 actcgaaaat gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt
    14281 aatgtggggg attattagtc gtggatagat agaattttag ttttttagag gaaatatgtc
    14341 tttaaaaatg gaatggttgc aattcttagc gatatatacg atgtaattgt cctcttaaaa
    14401 aagtatggaa aaataattgt attgggtgtt atgtataatt catggtgaac tgagactaat
    14461 gagaagaaaa attcatattg ttatgagtga gattgtatca gatgagaatg ataaattaaa
    14521 taggtgcata aaagcattac acttaaaata cattgttgct tacattatta caatatgtta
    14581 ataaggcaaa aataaataga tggattgaat aaacatattt tttaatcaaa tagtccctac
    14641 tgtgactttc ctcagctatc ggtgatacta ccgatagcat ttttttgctt ataaaataat
    14701 tataagtatt tagagatttt tcttctaaca ttcttcaaac aagaaaaatc acaaaggtta
    14761 ttgttatttt tatatattct ttttaagaat gattgttttt ttatagaaat tttaaaaaat
    14821 aaaacacgtt attgatggat agttagtttg ttatttggta atattataga agttgactgg
    14881 tagtgattta tttttcgtcg ttttataaaa atgttatttt ttggaaaaat tcacaaatat
    14941 gtaacgaatg tttttctgga atttgctgtg ttaaaaagca gtatgactaa tataaatact
    15001 attgtcattt atgcaatttt ttatggatag taattttaga ggggaatcaa tgaaggaaga
    15061 atagaaggga aaaatagttg caaatacgcc agttagcaaa aatagcaata catcttcaag
    15121 ctttaaaatt cgagagaatc atcttggtag tggaatcaac tacaacaaaa ggattaaacc
    15181 aattaataga tagaattacc aatggaaaaa tagaaaaggt agttattcta tacaaaagtt
    15241 gtttaattcg cttcggttat gaacttagtg aaaccctttg caatatatgg tacagaatat
    15301 agaaattatt gacattacag aaaatgatga agatttaata cagattgtca ctatttgtag
    15361 atacctttta caaggaaacc gagccaataa agctaagaag atgattaagg agttgatgga
    15421 agacgataca agttaagaaa gtgaaactac gcccaaataa agagcaaaaa gagtaattgg
    15481 acgtggaaaa tgcaagaaat gaaccatcga ttcggtggac agtttattag taatgacggt
    15541 ttgagaacat ccatgacgag aatgaaggaa catcctaaat gcgtttagtt gaaccaagtg
    15601 ccaaataata tggccaaata agcgatcagt tatcgatgaa tcccaagtat tctaaactat
    15661 atattcattt tgatgctaag cattgatata tttttttggg attgatgaag gttaaccaat
    15721 acagggactc actgatgaaa ttctagagtc tgaccttggt atcaacgatt tagtgattgt
    15781 ttctaatgga aaagtataaa agtatataag tacattaaca aaataactat tagggttacg
    15841 ctccagcttg tgcaaggcac tggcaggagg aatcgtgaaa tcataattat ggttaagcca
    15901 cacggacaac taaaagtcat acaaagctat cgaatcaatc aacagaagac aatcggcaat
    15961 cattagcttt gtttacagct tacattgcaa cgcttgagtt gaaccgaaaa tacgagggag
    16021 gtaacgcagc cattttgagt tggggaatct ttttagaaaa ttatcgggta ccttgttgtt
    16081 tagtcaaaat tataaggggg agtaatagtg aggaacaaat ttcataaact gttaagtatg
    16141 tttctagtta tgcttattgt gatcaatcaa tttgggaaca taacggttct agctgagtct
    16201 ttagcagaac agccagagcg ttcgctgacg atcgaagttg atcaagagga ggctgctttg
    16261 ggtgagcagt tccagttgcg agtcagagga acaccagacc tgctaaatgc attggtcatt
    16321 gataagagtg aggatttgat tgaaggggaa gtgaagcaac tcaatgatta cgaacgtctg
    16381 attacttatt cttcccagca ttcaggtaaa tacgaattga ccgcaagaag cggtgatatt
    16441 caatcaaata cggtctcaat agaaatcagt gtaccagaag aaagcacaag tgagtctttt
    16501 aatgtaccag aagaaagcac aagtgaatct tttaatatac cagaagaaag cttaagtgaa
    16561 tcttttaatg aagccagatc atctgagcca tctataacgc aggaatctca gattgaaaga
    16621 caagaggagc tcttgaatta tgacatcggc atctctgctg agcgccccac tttgccgagc
    16681 aggcatacac aaacgttaaa gggatacatt caaggaggcg ctggcgatat tcttgaagaa
    16741 tatggggaga tggtcgtacg agttcctaga gcatcgattg gcttaattga gttcattaga
    16801 gtggaggtcg ctgatgattc acctttaaag ccactggcat tttaccaaga aggcacagac
    16861 gttgtttttc catttacctt tgattatgaa ggattaaatg aagccgctca gtcgtataca
    16921 ttcagtatca cgtttgaagt tgctttcttt caatcaggcg gtactgagcc aaataatgac
    16981 tttcaagtga gtcttactta tgctgagcga gcagcaaaca cggaattttc aattacgcct
    17041 attccttcat caagacccat tttttcgaaa ttatactatg gctctagaga aatcgacagc
    17101 caaggaaatc ttgggaatct gctgattgac gggcaagacg tgaccaacag taataaattt
    17161 attttgcagg taaattatga aggatcacga aaagacggtg tagtggttac cgatgtacta
    17221 ccgcaaggaa caactttggc accgaatccg actacatgga gttccactgg aaatagagaa
    17281 cctatttcag gtggggttcg gatcgcagaa gtaacgaatc caggtgaagc aaacgttaga
    17341 actcattatg tttccgagga atttgtggac aaagtatctt ttaataagga aacgaataca
    17401 cttgttgtgg actttggtaa tctagcagag ggtcaatcgt attttattga atatgctctt
    17461 gaagtgaccg atgtcgactt gttcaatgaa ggaagacaag aggatgggtt ttctcggaat
    17521 cgagcgagta tgacctacaa taatcaggta atgagccgag actaccgctt gagaatccct
    17581 gaaagtaatc ttccggcaat ctctttaaga aaaagtgtgg acaaaaatgt tatcaatctc
    17641 aatgaaaatg aactaacgta tactctgcat gtctcgatgt tttctggaac aagaaatacg
    17701 cttgttattg aagatgtttt ggaagaaggc atggaatttg tgtcatttct atcagatatt
    17761 ccctctttca ttgatgccga agtcaataat ggaatagttc gtttcagtgc gaataatacg
    17821 attggtggtc cgacaagttt taccctctct tttaaggtca atgtagaggc gtatgcggtc
    17881 ggcgatcaaa ttttaaactt tgcaacgctt gtgaatagtg acaatcgatt gaatacgagt
    17941 actgtaagaa caaggaaaat cgatgggcgt attaaagtaa ttaaaactga tgagaatggc
    18001 caaaggctgg caggagcaaa atttgaaatt ttatcaagcg attcacaagt gatacaagaa
    18061 ggtataacgg cagaaaacgg agaattttta agtgaaccgc ttcctgtagg tagctatcaa
    18121 gtccgagaaa cagaagcacc ggaagggtat cttttagatg cgacggtaca tgatgttttt
    18181 ataaatgaat actccataga agaagtgacg ttaaccattg aaaacatcct tgatacgggt
    18241 ggtgttgaac taattaaaca tgctgaaggc agtggtgaag tccttcaagg agctgttttt
    18301 gagttacaga accgagaagg agaaacgcta caaaccggt
//
LOCUS       DACEMS010000050        18166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEMS010000050 DACEMS010000000
VERSION     DACEMS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.050"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..722)
                     /locus_tag="IW735_001520"
     CDS             complement(24..722)
                     /locus_tag="IW735_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1044527.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IW735_001521"
     CDS             complement(715..2067)
                     /locus_tag="IW735_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1044528.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCQHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IW735_001522"
     CDS             complement(2290..3690)
                     /locus_tag="IW735_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1044529.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IW735_001523"
     CDS             3851..4588
                     /locus_tag="IW735_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1044530.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IW735_001524"
     CDS             4713..5192
                     /locus_tag="IW735_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1044531.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IW735_001525"
     CDS             5189..5956
                     /locus_tag="IW735_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044532.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IW735_001526"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IW735_001526"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1044533.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IW735_001527"
     CDS             complement(6859..7461)
                     /locus_tag="IW735_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1044534.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IW735_001528"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IW735_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IW735_001529"
     CDS             8142..8726
                     /locus_tag="IW735_001529"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1044535.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IW735_001530"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IW735_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1044536.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IW735_001531"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IW735_001531"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1044537.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IW735_001532"
     CDS             10627..10806
                     /locus_tag="IW735_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044538.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IW735_001533"
     CDS             10828..11853
                     /locus_tag="IW735_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1044539.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IW735_001534"
     CDS             12028..13272
                     /locus_tag="IW735_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1044540.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IW735_001535"
     CDS             13425..14372
                     /locus_tag="IW735_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1044541.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IW735_001536"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IW735_001536"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1044542.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="IW735_001537"
     CDS             complement(15274..15516)
                     /locus_tag="IW735_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044543.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="IW735_001538"
     tRNA            15637..15709
                     /locus_tag="IW735_001538"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="IW735_001539"
     CDS             complement(15828..16466)
                     /locus_tag="IW735_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1044544.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="IW735_001540"
     CDS             complement(16589..17221)
                     /locus_tag="IW735_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1044545.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="IW735_001541"
     CDS             17572..17871
                     /locus_tag="IW735_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1044546.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6240 a         3164 c         3576 g         5186 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgctg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa
    18121 tatagattga aaaaatgaaa gcaataagta aaataaaatg aatata
//
LOCUS       DACEMS010000051        17546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEMS010000051 DACEMS010000000
VERSION     DACEMS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.051"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..970
                     /locus_tag="IW735_001542"
     CDS             83..970
                     /locus_tag="IW735_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1044547.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IW735_001543"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IW735_001543"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1044548.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IW735_001544"
     CDS             2272..2718
                     /locus_tag="IW735_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1044549.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IW735_001545"
     CDS             2991..4343
                     /locus_tag="IW735_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1044550.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IW735_001546"
     CDS             4514..4801
                     /locus_tag="IW735_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044551.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IW735_001547"
     CDS             5027..5470
                     /locus_tag="IW735_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1044552.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IW735_001548"
     CDS             complement(5476..6660)
                     /locus_tag="IW735_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1044553.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IW735_001549"
     CDS             6928..7626
                     /locus_tag="IW735_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1044554.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IW735_001550"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IW735_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1044555.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IW735_001551"
     CDS             complement(8436..8969)
                     /locus_tag="IW735_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044556.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IW735_001552"
     CDS             complement(9266..11647)
                     /locus_tag="IW735_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1044557.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW735_001553"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW735_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1044558.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IW735_001554"
     CDS             12348..12887
                     /locus_tag="IW735_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1044559.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IW735_001555"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IW735_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1044560.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IW735_001556"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW735_001556"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IW735_001557"
     CDS             13994..15160
                     /locus_tag="IW735_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1044561.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IW735_001558"
     CDS             15206..16702
                     /locus_tag="IW735_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1044562.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IW735_001559"
     CDS             16783..17466
                     /locus_tag="IW735_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1044563.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         5931 a         3018 c         3631 g         4966 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttac
//
LOCUS       DACEMS010000052        17258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEMS010000052 DACEMS010000000
VERSION     DACEMS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.052"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(273..2180)
                     /locus_tag="IW735_001560"
     CDS             complement(273..2180)
                     /locus_tag="IW735_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1044564.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2310..3185
                     /locus_tag="IW735_001561"
     CDS             2310..3185
                     /locus_tag="IW735_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1044565.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3265..4239
                     /locus_tag="IW735_001562"
     CDS             3265..4239
                     /locus_tag="IW735_001562"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1044566.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4428..5564
                     /locus_tag="IW735_001563"
     CDS             4428..5564
                     /locus_tag="IW735_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1044567.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5701..6045
                     /locus_tag="IW735_001564"
     CDS             5701..6045
                     /locus_tag="IW735_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1044568.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6316..7602
                     /gene="efmA"
                     /locus_tag="IW735_001565"
     CDS             6316..7602
                     /gene="efmA"
                     /locus_tag="IW735_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1044569.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7740..8339)
                     /locus_tag="IW735_001566"
     CDS             complement(7740..8339)
                     /locus_tag="IW735_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1044570.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8743..9864
                     /gene="mnmA"
                     /locus_tag="IW735_001567"
     CDS             8743..9864
                     /gene="mnmA"
                     /locus_tag="IW735_001567"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1044571.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            10110..11531
                     /gene="arcD"
                     /locus_tag="IW735_001568"
     CDS             10110..11531
                     /gene="arcD"
                     /locus_tag="IW735_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1044572.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11545..12663
                     /locus_tag="IW735_001569"
     CDS             11545..12663
                     /locus_tag="IW735_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1044573.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12723..14015)
                     /locus_tag="IW735_001570"
     CDS             complement(12723..14015)
                     /locus_tag="IW735_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1044574.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14165..14725
                     /locus_tag="IW735_001571"
     CDS             14165..14725
                     /locus_tag="IW735_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1044575.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14854..15231
                     /locus_tag="IW735_001572"
     CDS             14854..15231
                     /locus_tag="IW735_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1044576.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15164..17236
                     /locus_tag="IW735_001573"
     CDS             15164..17236
                     /locus_tag="IW735_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1044577.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         5513 a         3129 c         3671 g         4945 t
ORIGIN      
        1 aggtcaggac gctgatttca caacctctcg ataaacggtg ttcaaaagcc agtttctcgg
       61 tagtaagtca aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttactac
      121 ttgaaactaa gcagcttttt cacaacctct aatacatggt attccagaag taacttcttc
      181 gaaaaataag ccgaaatgtc acaaaaattt gagaagcgat tttcccaaat tccttcttat
      241 ttcttgaagc tgaccacttc tggcacaacc tcttaccaga ttcgcactct tttttctggc
      301 gctagataca tagcgtcttg ttccgtgata ccaaatgttt catagaattc gtcaaggttt
      361 tttggttgca cgtttgctcg gagtttagca ggggcatgga catcgatttg cagtaaaagt
      421 tgttggtatt ctttttttgc ttttgtacgc caaatcgttg cccaattgac aaagaattcc
      481 tcgatagaaa gatctgattc cgtttttgct gcttccaatg cacagcttag accgcctgca
      541 tcagcaatat tttccgaaac ggttaatttt ccgtttactt ttccgtcagc aaaaggcaga
      601 ccatcgaatt cttcgatcat tgcttgtgct ttgttttgaa attgtgtaag atcttcttct
      661 gtccaccagt tgttcaagtt gccatattcg tcaaataaag cgccgttatt atcaaatgca
      721 tgggaaatct catgagcgat aacagctccg attccaccat aattagcaga gcttgattgt
      781 tctagactgt aaaatggtgc ttgaaggatc gctgccggaa agacgatcac attccgaaat
      841 ggatgataat aagcattgac cgtgtcagca ctcatttccc attctgaacg atccactggt
      901 ttgttccatt tgctgaaacg atcttttaat gtgagtttgc tgaaaacaag agtatttgaa
      961 agcaaagtac caccctgatc tgcaggtact gttttgaact ggtcgaataa tgctgggatc
     1021 ttatcggggt agcctacttg gatacctaag ttatttaatt ttatgacggc tttttctttt
     1081 gttgcatccc caagccagtc attttcttct agacgttttt tataaacggc gatcattttt
     1141 ttcaccattt gttctacgtc ttttttagca gcttcgccaa aatatttttt tccgtagtaa
     1201 tcgccaacta cttgatcgaa ttgtccagag gcaaggtaat acgctgcttt tttctgaggc
     1261 attgcttcgt ctgttccaga caatacgcga gaatagattc cgctgacttg gcggaattct
     1321 tctgatagat aaccactaag agaacgtact gttttaatga tcatccagct tttgaataac
     1381 ggaaaatgag cttctgtcag gatgtcattc aaatgatcga agtacactgg atcagttacg
     1441 atcactttat ctggagttgt cccaatcaaa cccgtgacaa gttttttcaa gtccagttta
     1501 tctgtgtatt taataaatgt atcaaaagat tgcggattat acattttgct gtaatctgcg
     1561 ttttcttctg cactttttac atgaggagca atcaatttat caaattgcac tgcttgttcc
     1621 gtgattgctt ctgcctgact tttttctttg ccggcaagct ctaataactg aacagccata
     1681 tcaaagaaaa cgcctattag ttgtgcaccg ttgggatggt ctgatgcata atacgtctta
     1741 tctggaagga ataaagaagg cggataagca aagaaggcgt tcgtttgcgc attcttcata
     1801 tctgcatcta catccagatt aaatggcaat ggtaatgtat ccaacagcca ttctggaaat
     1861 tgcttattca attcgtcgaa agactgcaat gattcaactt tttcgattat aggttgaagc
     1921 ggttgagcat gcaactcatt tcttcggtca taatcgtttg ccagttgata atatgcaagg
     1981 aaatgtttca ttttttctga agacactttt tctggatggg caagcagatc gtctgtttct
     2041 cccatcaaaa gttggtcgat cccgtcaact aaatcttgga aaccgcctgt agctggttta
     2101 tctgcaggga tttttgctgt tttttgccat tcttcattga ctgcttcata gaaatcttgt
     2161 ttcaatcgtt ctttgttcat tcatcgatcg ctcctttttt gtttcgtgtg cccattatct
     2221 tacaaaatga aggaattaac aaatagaaca taaaaaattg aaaattcgaa caatcaaact
     2281 gatttgtttt tttcaatcct tcacttataa tgaaaaaaag gaggcgatcc caactgaatc
     2341 aagtagtgga taaaccttta tatttcaaag tagataaaaa aattaaaaaa ttagccagca
     2401 ctcagcagct gcagtcaaga aaaagtaaat tgcttttttt agctctggtt tttgaagatc
     2461 aaagttatgt gattatcgat caatcaggac atccggtaga atattctcca gcaaaatata
     2521 cttatcaaga gggtctttct tgtaaaaaat ggaagctgat caatgaagag ccaatcgagt
     2581 tatctagatg gatcaacaga aaagaagata ttcccgtttt gatcgaagaa aaaaggtcag
     2641 aaaaggaact cgcaaattgc tgggtgggat tgccagaaga acgttttttg aggtataaga
     2701 aatgggctac cccctcaggc tatctttgtg gtacttacgc ggcagcagtt ctccttgcct
     2761 attatcaaga ctatcgtaag gggtggatgc ttccaaatga gattcgcaag aaaaatacgg
     2821 ctgattcgtt ggttttgacg aaggcattgc gtagccagat ccaaccacta ggacttccta
     2881 cgattccttt tcaggttagt acagggattt ctagtttttt aaagaagaat ggcaatcatg
     2941 agcgtgcacg agcaactctt ctaggttcat ggcagcgagc tacaaaacgt atacgtgaag
     3001 gcaaacctgt gatgattggt attttgaaag tacttggcag tacatatggc aatcattggg
     3061 tgacagcgta cgcttatttt gaaactgaaa caggagaacg ttattacaaa gtacacgaca
     3121 actggggcga ttatcataaa gtcattcctg ccagttggag taacggaaca gtttcacttc
     3181 cttaagaatt tgctgaaatt aacagaaaat cttggaaaaa tatgttaaac tatcgaagaa
     3241 tagagaagtt ttggggagga ctacatgagt aataagtatc aggatgacac gttgaagatt
     3301 tttagtttga atggcaaccg tccattagct gaaaaaatcg cgaaagtgtt cggaacagaa
     3361 ttaggtaaaa gcgtcgtgaa gcaatttagt gatggcgaga tttccatcaa tatcgaagaa
     3421 agtattcgag gggatcatgt gtatatcgtt caatcaacga atgcacctgt caatgattac
     3481 tatatggaac tactgatcat gatcgatgca atgaagcgag caagtgcgaa aacgatcaat
     3541 gtcgtattgc cttactatgg gtatgctagg caagaccgca cggcgaaacc tcatgaaccg
     3601 atcactgcga aattgatcgc taacttgatc gaagaagcag gggcaactcg agtactgact
     3661 ttagatctgc acacggttca agtacaagga ttctttgata ttcctgtaga taatttattt
     3721 acgatgccgt tgtttgctca ttattaccgt caattaggtc tgacgggaga tgacatcgta
     3781 gttgtttcgc caaaaaacag cggtgttcag cgtgcacgta gtctttcaga atatttgaac
     3841 agcacattag ccatcgtaga tcatgcagat gatgcttcaa ctgagggtgg ttatgtgatc
     3901 ggtgatgttc aaggaaagac atgtatcatg gtagatgata tcttgaacac aggagcaacg
     3961 cttgcacgag cagcaaatgt attaaaagaa aacggcgcaa aagaagtata tgcctgtgcg
     4021 tcacatggat tattgtccga tcatgcgaaa agtattttag acaatgcacc aatcaaagat
     4081 atttgtatca ctgattctgt tttcacagaa gaaaatcgtc atccagaaaa cttaacaatc
     4141 gttacatgtt cagaattaat gggtgaagcg gtcaaacgca tccatgaaaa tacaccgatg
     4201 agtccgttat tccgtctgga agaaaaagaa ttcgaataaa caaaaaaaag agggaaagaa
     4261 gttgtgacag aagtgagcag cttcaaggag ctgcgacaag acttttgtca cagctccttt
     4321 tttctatgcc catgaaacgt cctctagaca aaaatgagct cttctttggt ttgttcgcta
     4381 ttttatgcta gaatgtgcct agcaataatt tggaagggat gaatgaagtg aaaactattt
     4441 atctggatca tgcggcaact acccccatgc atccacaagt aattgaagcg atgacggcaa
     4501 tcatgcaaga cacgtttgga aatccttcaa gcatacatgg atttggtcgc cacgcgcatg
     4561 aaaaattgga aacagcaaga caaatcattg cagacagtct gcaagcaaaa cctcacgaga
     4621 tcatctttaa cagtggggga acagaagggg ataatacagc tatcttagaa acagcgttct
     4681 cgcgtcaaaa agaaggcaga catctaatca ctacagctat cgaacatcct gctgttctta
     4741 ataccatgaa atatttagag acaaaggggt ttgacgttac atatctgcct gtagatgaaa
     4801 aagggaatct gtcgatcgaa gaggtcaaaa aagcgttacg agaagatacg atcttggtct
     4861 ctgtgatgta cgggaacaat gaaatcggaa atttgatgcc aatcaaagaa atcggtgaac
     4921 ttttagcgga tcatccagct tatttccata cagatgcggt acaagcgtac ggaaaccaaa
     4981 cgatccatcc agaagaatta ggaatcgatt tattaagtat ctctgctcat aaaatcaatg
     5041 ggccaaaagg cgtcggtttc ttatataaac gagacggagt aaatatccct gcattgcttt
     5101 taggcgggga gcaggaagaa aagcggcgtg caggtacaga aaatttagca gggatctggg
     5161 gtatggcaaa agcagttgaa ctgctgtcac ctgaagaacg tgaagcacgt aacaacaaat
     5221 ataaaggatt ccagcaattg attcttgata cattagaagc aaacgaaatc ccttatcaag
     5281 taaatggtga tttaacgaat aaactccctc atgtgttgaa tcttcgctta attggcgaag
     5341 caaatgattt gctactgatg aagcttgatc ttaatggggt cgctatttct actggttcag
     5401 cttgtacagc aggaaatatc gagccttcac acgtattaga agcaatgtat ggagaagata
     5461 caccgatact aaaagaatcc atccgtatca gctttggttt gggaaatacc gaagacgaca
     5521 tccagtattt ccttgatcaa ttagttcgtg ctgtcaaaaa ataactagtg aagtcattct
     5581 tatatgaaca gatgtctgag agaacgcctt cattttttag ggaacttaac tgaaaaacct
     5641 agtaaaaatc aggataaact tttataataa agagaaacta attttatcga ggtgaaaatc
     5701 atggcatttg aaacaacagc aacggtactg ggcgcaccaa ttagctatcg tttgcatcct
     5761 gatgcaaaaa gatatacatt gcgcgataat ggatttacag aaacaaacgg aggcaattat
     5821 caattgatcc gaccattaga tgccacgccg caaagcaaag aaggattcaa attaaaaatc
     5881 actgtatcaa aagatattca aacattgaaa atgtcgatca ctacagcaaa tggattgaaa
     5941 gcagtcaata ttttcaaaga tccaaaacag aaaatgagcc aagataaatt ttattttctg
     6001 atggatggga tgattagccg tgggttattc gaaaaagtcg aatgatctgt ggatataaaa
     6061 agacaactag ttttgaaacg gcctattgtt agatgactaa ctttgtaagg acgacgcaaa
     6121 ttagttgtct tttttatttt taaatttaac acatgttgtt atttttaata ttttgctgat
     6181 ttattgtttc taagtaaaga aatttaaatt gaaacgattg ttatttaggg aaaaaggtat
     6241 tttttgaaaa aagaatcccg tataatgatt gttgtgcaaa cgctatacaa aatgaaaaag
     6301 aaaagaggga tgtttatgga aaatgaacag tcggtcgttt taacgaattg gaagagaaat
     6361 tatttgttct ttttatcggg acagtttttg tcaggaatca ctagtatggt cgtccaatat
     6421 gcgatcatct ggtatttgac aagagaaacc ggttcggcga caattttgag ttttgctact
     6481 ttactaggaa tgattcccat ggttttacta agcccatttg taggaccgct agtggatcgc
     6541 tgggataaaa aagctctttt gattgtcacg gatattattg tagcgatttt cgcgcttatt
     6601 ttagcagtag tcggaacgat ttcagaatcc ttcccgattt ggctagtttt tgtgtctttg
     6661 tttatgcgtt cggtcgcaca aacattccaa atgccaacga tccagtcgat tatgccgaca
     6721 atcgttccat cttctcacat aacaaggacg aatggacagt taggaatggt ccaatcagcg
     6781 aatttcatca ttgcgccagc tctaggtgcg gctttgtttt cagtcgttcc tgtcaattac
     6841 cttattttat tagatgtatt gggggcagtt tttggtgtcg gcttattgat ttttgtgaag
     6901 atcccaaaag tttctcctga aatattggaa gttccgctta ctatttttaa agatgcaaaa
     6961 tttgggctgc agcaattgat ggataataaa gggttatggt acataacgat caatggtgca
     7021 tttgtgatgc tgttgttcat gccagcaatc agtctgtatc cattgatgac gctagattac
     7081 tttggtggtt ccgttggaca ggcgggagca gtggaagtag tctacgcagt cggaatgctc
     7141 ttaggcggtg cactgatcag ttttatagga acatggaaag accgaatgaa acctattatt
     7201 atagcctaca tcattatggg cctgacgatc ggtgcaagcg gattggttcc aaacgatagt
     7261 caagggttct tgtactttct tatcttgaat gcaggagcag gttgtgcaac gccatatttc
     7321 aatacactgc tgatggcaat gatccagcag agttatgaat cgaatgtttt aggtcgtgtc
     7381 ttagggaact tcaactcttt gatgaacctg gcaggtccaa ttggattatt atttgcagga
     7441 ccattagctg atcgtttagg ggtagaaaaa atgttcttgt tttccggaat cgggatttta
     7501 ttatgcggaa tcgttttatt tttaacgtca gctgcccgaa attatgataa agaattacag
     7561 aaaaaactgg taaaagaaca ccatgagcaa aaagacgaat agtatggaat ggagttgtga
     7621 cagaagtctt gttacaactc catttctgat cctaatgttc ctgtggcaat aagcctactc
     7681 aaatgaaaaa tatgagtaat tatctttatt tgtttaatac ttgatatagg gcagtttttt
     7741 cacgacctat cgtacttcct ctttgcttca tagatttctt tgatttgttg ctcagataac
     7801 ggagtgccaa atggtagttt ttcctttaag agttctcttt ccggtgcgtc tacgcctaca
     7861 ttgacatcgt tctcgatagg tacgctgtaa tgatggatat gtccatgaag attgcggata
     7921 gtgtcgttag gcccgagcaa catcggataa tgagtcaagt aatattgttg atggtcgaat
     7981 ttaacgatgg cacctacatc ataaaaataa aatttgggac ttccgtcagt aagcctagga
     8041 tcattttttt ctaaataacg gaaaaaagcg cgactgtcat ggttcccttt gatcaagtgg
     8101 attgttcctt gcagttgagt gaccagttcc aaaatttctg catttgcttt ttcatattgc
     8161 ggatgcatag caaggtcgcc tagatgataa acatggtcgg tttcttttat tactgcattc
     8221 cagctagtga tcaactgttg gttcatttca tccacatttg caaagagccg cggagcaaag
     8281 tcgttttttc ctaataagcg ttcatggaaa aagtgcatat cactgataaa gtaattcatg
     8341 tatcttttgc ctcccttttt tcttagagta aacaaaatca cttatctgag caactcataa
     8401 catctaaaaa gaaaaggttg taaatgagac gtgcagcttt aagcgataag aagcaatttt
     8461 gaaaaatagt ttttcacaat ttataaaatt attccttatt gccgaaaggc tggccctttt
     8521 tatgagtatg ccaatttttt atatcgaatg ctcaaaagat gagtgatttt ttggttgact
     8581 atcatgtttg tgtactgaat aggtccagta gaacagtatt tttacgactt gataattaag
     8641 ggatgaaatt ttcagaaata tgacgtataa tgtaaaatac tgaaaagttg cgaccttcaa
     8701 ctcgtagatg tttcagaatc taattgaaat gatggtgata tcatgacaga caacagcaaa
     8761 acacgcgttg tcgtagggat gagcggcggt gtcgactcat ctgtaacagc acttttgtta
     8821 aaggaacaag gatacgacgt gatcggaata ttcatgaaaa attgggatga cacggacgaa
     8881 aacggtgtat gcacggccac ggaagattat aaggatgtag cgaaagtagc tgctcaaatc
     8941 gggattcctt attactctgt gaattttgaa aaagaatatt gggatcgtgt atttgaatat
     9001 ttcctagccg aataccgcgc aggacgtaca cctaacccag acgtgatgtg caacaaagaa
     9061 atcaaattca aagcattctt ggattatgca atggatcttg gcgccgatta tgtagctact
     9121 ggacattatg cgcaagtcac tcgcgatgaa aacggcgtgg tccacatgct tcgtggagta
     9181 gacaatgata aagatcaaac ttacttcctt agccaattat cacaagaaca gctttcaaaa
     9241 acgatgttcc ctctaggcgg catggaaaaa tcagaagtac gggcaattgc tgaacgcgca
     9301 ggccttgcga ctgctaagaa aaaagattca acgggaatct gctttattgg tgaaaagaac
     9361 ttcaaacaat ttttgagcaa ctatctgcca gcaaaaaaag gaaatatggt cactcttgat
     9421 ggtgaagtca aaggacaaca tgctggcttg atgtattata cgattggtca acgtcaagga
     9481 ctagggatcg gtggcggagg agattcgcaa gaaccctggt ttgttgtagg aaaagatcta
     9541 gcgacaaaca cattatatgt ggggcaaggg ttccatcatc ctgcattgta tgccacaagc
     9601 ttggatgcaa gtgaaatcca ctttacgacg aatgaaccaa tgccaaaaga attcaagtgt
     9661 acagcgaaat tccgttatcg tcagcaagac gttcctgtga cagttcgctt attggatgat
     9721 aatcgtgcag aagtcgtatt cgacgaacct gtacgtgcaa tcactcctgg acaagcagtt
     9781 gtattttatg atggaatgga atgtctgggt ggtggtttga tcgaccacgc atatcaagaa
     9841 acaaaagttt tacaatacgt ttaagcgata ttaaatgaat caaaacagct atggctaaca
     9901 aaagaaacac cttttctttt ggatagcata gctgtttttt tgcaataaaa ttaattttta
     9961 ttcattttat acttgaaata cttgtgtaaa aagttcataa tatccaataa tatgaataaa
    10021 gttgcttttt gttaagcaag agcagttgca aaatagaagg gaatcttttg caagtgattt
    10081 gttcaatttt ataagtgagg gtgatggaaa tggaaaagaa atcgacgggt attagcaaat
    10141 ggggactggt tgctctggtt gtcagttcgt caatcggaag cggtgtgttt gggatcacaa
    10201 gtgatctggc tagttctgca gctccagggc cagcgatcct ttcatggata atcgttggga
    10261 ttggtatttt agctttggta ttatcgctga atcatttagg agaaaagcgt ccagatctag
    10321 atggtggtat tttcggttac gcggaagctt catttggtaa attaggtggc ttcatcagtg
    10381 gttggggtta ctggttgtct gcgtggctgg gaaatgttgc ttttgcaacg atgctgatga
    10441 gcgccatggg agaatttttc ccgatattca aaggtggaca aaatgttcct tctattttat
    10501 ttgcaagtat ttttatttgg ggtctgactt tattagtaaa caatggagtt gaaggagcga
    10561 gcgtcatcaa tacaatcgtg acgatctgta aattagtgcc attattccta tttttaatat
    10621 ttgtatttat cgcatttaaa gtgaatatat tcactgctga tttttggggt aacgtatctg
    10681 ataatctagt gaatggaaca gggcagcaag gaacgatctg gttgcagatt aaaggttgtt
    10741 tgatggttat gatgtgggtc tttgtaggga tcgaaggcgc aagtgtttta gccaaccgcg
    10801 cgaagaaacg ttcagaagcc cagcaagcat cgatcattgg tctgctttgt ttattgttta
    10861 tctatatcct tgcttcgatc ttgccatacg gcgtcttatc tcaagaagag ttagcaacta
    10921 tttcacaacc tgctatggca aatattttaa aaggaatcgt gggaaattgg ggagcagcgc
    10981 tgatcaatat cggcttgatt atttcgatca ttggttcttg gttgtcttgg acgatgctcc
    11041 cagcagaaac aacgatgttg atggctcgag acggtctgtt gccgaaaggt tggggaaaaa
    11101 cgaataaaaa aaatgcccca acttattcgt tagttgttac tgcaatcttg actaatctat
    11161 tcttattgac tttcttagtt actgattatg cctatcaatt tgcctattct ttatgtacag
    11221 cggcaatttt gatttgttat ctacttgtcg ggatctatca agtccagttt tctttaaaag
    11281 aaggaatcaa aaaacaagta gtgatcggtg ggattgctgt agtttttgaa ttgatgggca
    11341 tcattttagc aggattttct tatgtccttt tatgtagtat tgcctatctt cctggttttt
    11401 gcttctactg gatcgcttgt cgtgaaacca aacatcaaat tacgggaaaa gaaaaagtaa
    11461 tgattgggct gatttgtgtg gccgcactgc tttcaatcgg gttgcttgct gtaggctgga
    11521 tcaagatata agggggaaag aaacatgaaa atcgcaaatt tcggtgtaga agaatggcta
    11581 aataaatggg aaaagaaagc ggtatatgat attgcacaaa gctcgatcga agcattaaca
    11641 ttagacgaac tcgttggctt agatgggact agcgtaagta aatttttcga aaaacgcaaa
    11701 aacgaaccat tggactatgg ctggatcgaa ggatcagatg ttttcaaaca agaagtcgct
    11761 tctctttatc aaaccgtcga tccggaaaac atcttacaaa cgaatggagc gactggtgct
    11821 aatcttttag ctctatacgc gttagtggaa gcaggagacc atgttgtttc gatgcttcct
    11881 acttatcagc aattatatga catccccaaa tctttaggtg ccgctgttga ctttgttcat
    11941 ttgaaggaag aagaagattg gcagtttgat ttagaacagc tagaagcctt agtcaaacct
    12001 gaaacaaaaa tcatctgttt gaatagtgca aacaatccta caggcacatt gctagatcgg
    12061 aaaactttag aaaaaatcgc agaaattgct cgtacagtag atgcatatgt attgatagat
    12121 gaagtatatg cgcctttaac ggataaaggt gaattcttgt cgatcgtaga tgtctatgaa
    12181 aaaggtatag caaccaattc cctatccaaa acctactcca ttccaggtat tcgtattggc
    12241 tggacagcta ctggacctga gttggcagaa gtttttcgaa agtatcgaga ctacacgatg
    12301 atttgcggcg gagtcctgtc agatgatctt gctgtacatg cattgaagaa taaagaaaaa
    12361 atattggcgc gaaacaaaaa gatcattaca gagaaccttg tgattttaaa acaatgggta
    12421 gccaatgaac cgaaagttga acttgtcgct cccaattacg tctctacttc tttcattaaa
    12481 ttagccatcg aagaagatga ccagacattt tgtatcaatt tattagaaga aacaggagta
    12541 ctattagtac caggaagtgc ctttgattta ccgaagcatg cccgactagg ttactgttgt
    12601 aaaaaagaaa ccttggaaaa gggattattc ctcttatctg agttcttgaa aaaacaggcc
    12661 taaacaaaaa cactgcaagg acagtctgtg acaactgttc aagcagtgtt tttcatttac
    12721 gcttattgtc tatgagaagg agagtgccct gaagctatgg agactcctga agctttttct
    12781 tctgcagttt cttgctgatg gatctcttgt gtattgtgtc gtacaagcaa gaagttgacc
    12841 aaaacaagaa gagaagtcga aagaaagacg ccacgataac caaatgcact ggaaacactg
    12901 gagccgatca taggccctac tacgttacca gttgcttgga aagattggtt ataactgaag
    12961 atccgtccag cagcttgatg aggcgaatat tttgtgatca atgcttggac agcagggagc
    13021 agacaagcat cagagatacc aatcaaaaaa cgtaaggcag ctaattgcca aacattcgtg
    13081 acgaatgcca ttgggatata aaccagaatc gcaaaaccca aaccaatcgc aagtattctt
    13141 tcactcccaa tcttgtcacc taagcgacca aatcttggag cggctatcaa agtggcaatc
    13201 ccaggtatcg aagcaatgat tccgctgatc aacgtaacat tcccatgtcc atgcagcaat
    13261 tggcgaatgt acagactgat gattggacta atggaattgt tggatgcttg gatgatcatc
    13321 gttgtgacga acataccgat gacgacgtgt ggatatttca agtctttgaa aatctgtttg
    13381 gcagaaacca tgttttcttt tttcactggg acaaattttt catggacgaa aaacagactc
    13441 aacaagaaga cgaaaaataa gatgatccct gtaatgaaaa aggtagggcg atagccaaac
    13501 gctgaagcag agatcccgcc aagaagcggt ccaagtaatg ttcctgtaac agaaccagtt
    13561 gctaaggttc caagaacttg accacttttt tcttttggcg tcccagttgc gacaagggca
    13621 gtggcattac tgatataacc agaaaaaatc ccttgaagca gacgcaatgc gactaattga
    13681 tacacactag tcacgcaacc catcaaagaa atgacgattg ccattcctaa tgaagcacgc
    13741 aacagcatca attttcttcc tttttggtcg gctaattttc cccaccaagg agagataagc
    13801 gtagtcacca agaaagtaga tgaaaaagtc aatccgctcc aaaagttcaa ttgtgacgta
    13861 ctataattac ctaacgtatc tatgtaaagt gacataaaag gcatgactaa actaaatccg
    13921 ataccagcca taaatgttcc aaaccataag acaaacaagt tttgttccca agtttctctt
    13981 ttcctaaata atttttcctt tattcttgca gccaattaga ctccttcttt ctgaaaattt
    14041 tcatctacag ctctttatta taaactacaa tatgtagttt atcaatgaaa gtctcttttt
    14101 tctctttctt tttcgtggta aactgaaaga gtgaaggatt tattttatga gaggaaacat
    14161 ggaaatgaaa aaaagaaatc tgtctcaaga gaaaatcatc gactgctttc gagaactagc
    14221 agaagagatg gatgttcagc aaataacctt tcagcattta gccaaacgct tggatatcaa
    14281 ggctccctcg ctgtataacc atttcaaaaa tattcgtgat gtcaaaacag cgctaacagc
    14341 gaaacttctg caggaactta atgaccagct gcgacgggct cttgtgggaa aaagtggtca
    14401 ggaagcactg cagatctatg cagaaactta tcgatccttt gctttttcaa atcaagcagt
    14461 gtatgagtta ctgatcagtg taccgcatac caatgaagct gttttattag atggaatcca
    14521 tgaaacgaat cagatcattt tgcagatatt tgaggctttt caattaacga aagaagaaaa
    14581 agttcatcga agcagggaac ttcgtagtat gatccacggt tatctttcct tacgtttttt
    14641 aggatacttt acaaaagaac caacaatcga tccagaagac agctattttt ggatgatcaa
    14701 taactttatc gctactttgc cttaaacaat cagattcatt tgtaacaatt ttaagatgtt
    14761 cattaaatga cccttttata agagaaaatt cttgcaactt tcttgaaaag tgactaagat
    14821 gagtctagaa aagctgaaag aaggagcacg agtatgtatc aagtaattac catgttcggg
    14881 gacaatgagc cttggtggtt ttttgaagac tgggaagaag atattgaaga agaagaaaca
    14941 ttcgagtctt tcgaagaagc acgacaagcg tacgaaaaac aatggaacga aatccatcaa
    15001 aattatgaat atatcaatgc aaaaagtaac tttctcagcg ccttttggaa tgacggagat
    15061 gagcgctggt gtgaagagtg tgacgatgat ttacagcaat acaagggact tgcgttgttg
    15121 aaaaatcatc agcccatcac agtagaaagc agaaaggaat tttatgaaac aactaattct
    15181 agcggaaaaa ccaagcgttg cgaaagacct aagcaaggtg cttggtgcta atcaaaagca
    15241 caaaaattat tatgaaggac ccaaggtgat cgttacttgg gcccttggtc atttattagg
    15301 attgaaaatg ccggaagatc tgaataagga atggcagagc tggcaaatgg acacgttgcc
    15361 aatgatccct aaaaaactag gaatcaaacc acttcctaaa acaggacatc aattaaaagc
    15421 aatcaagcag ctggctcagc gaaaagatgt ctcagaagca gtcattgcga cagatgccgg
    15481 aagagaaggg gaacttgttg ctcgctggat cttagagtat gtgcatttca ataaaccagt
    15541 gaagcggtta tggatctctt ctcaaacaga taaagcaatc aaaactggat tcaagcaact
    15601 aagacctgcg aaagaatatg atgcattgta tgaatctgct cttgcccggg caaaagcgga
    15661 ctggctggta ggattgaatg tgaccagagc cttaactgtg aagtacgcgg ataatttatc
    15721 agcaggccgt gtgcagacac cgacactagc attagtgaga gaccaagaaa agaaaatcga
    15781 gcagttccgt ccacaaacgt atttcacgat cacgttgcaa gctggaaaag aacaagccaa
    15841 aatgcttcaa aaaaatcaat ttgctttaaa aacacaagaa gaagcacaaa aacttgttca
    15901 attgatcagt acaagcaaag gaactgtcac agaagtccaa gaaaaattaa aaacggaatc
    15961 tgcgccattg ccttatgatt taacagagat ccaaagagaa gccaatcaac gctatggcta
    16021 ttctgcaaag aaaacattaa gtcttgtaca aagtttgtat gagacccata agatcgttac
    16081 ttatccgcgt acagacagca aatatctgac aaatgacatg aaaagtacga tgaaagaacg
    16141 attgcaagca gtcagcgact ttgctcctga ggtcaagcag tatttgaaaa acggagccat
    16201 cgtccaacag acaaaagtat tccaagacag taaagtgacc gatcaccatg cacttttgcc
    16261 aactgaaaac agagcacgtt atgaaaaatt aagtaatgaa gagcgaaaaa tctatcaaat
    16321 gattgtctct cgttttttag gactgtttgc tcaaccccat aaagtcagcc aaacaaaagt
    16381 tacggttgaa tttggtaaag aacgattcat ttttcggcaa aatcgtgtcg tccaagcagg
    16441 atggaaaggc gaaactgaaa ctgagacgga aacagtcaaa tgggaaaaag gaatgagaat
    16501 caatcccgat ttcgccatca aaaaagaact atctgcaccg cctaaaccat tgtcagaagc
    16561 aagcttgctt ggtcaaatgg aaaaatacag tctgggaacc ccagctacgc gtgcagagat
    16621 tatcgagaaa ctaatcaaat ctgaactgat ggaacgtacc cctcaaggac tgcaagtatc
    16681 tcctaaaggt aagcaattat tagtattggt aaatccctct ttagtaacac cagatttgac
    16741 agaaagctgg gaaaaagacc ttgaagctat tgctgcagga aagaaacaag caggaacctt
    16801 cttgaatgga atcgaaaaag aaacaaaacg cttggtgaat gagatcaaat caagcaaaca
    16861 agaatatcaa gatttctcga ttacccaaaa gaaatgccca gaatgtgggg ccaatctgcg
    16921 cgagaaaaac acacgtgacg gcaaaatcta tgtctgcacc aattcagatt gcaactaccg
    16981 cagaagaaaa gatccaaaag tctctaatca tcgatgcccg caatgtcacc ggaaaatgga
    17041 aatcatcgaa ggtaaaaacg gtgcttattt ccgttgtaaa tacgatggaa cgacagaaaa
    17101 attgcttgat aaaaaagagc agaagaagaa aatgaccaaa catgaagaac gacgcttgct
    17161 gaaaaaatat gcacaagcag aagaaccaga agaaagccca ttagcagcag cactaaaagc
    17221 agcaatgaag aagtaataag atatgaaaga ggttgtga
//
LOCUS       DACEMS010000053        16638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEMS010000053 DACEMS010000000
VERSION     DACEMS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.054"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..783
                     /locus_tag="IW735_001574"
     CDS             <1..783
                     /locus_tag="IW735_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1044578.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            1161..3203
                     /locus_tag="IW735_001575"
     CDS             1161..3203
                     /locus_tag="IW735_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1044579.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            3214..3834
                     /locus_tag="IW735_001576"
     CDS             3214..3834
                     /locus_tag="IW735_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1044580.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3846..4304
                     /locus_tag="IW735_001577"
     CDS             3846..4304
                     /locus_tag="IW735_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1044581.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            4323..4601
                     /locus_tag="IW735_001578"
     CDS             4323..4601
                     /locus_tag="IW735_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1044582.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            4626..5993
                     /locus_tag="IW735_001579"
     CDS             4626..5993
                     /locus_tag="IW735_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1044583.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            6008..7153
                     /gene="dgoD"
                     /locus_tag="IW735_001580"
     CDS             6008..7153
                     /gene="dgoD"
                     /locus_tag="IW735_001580"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ1044584.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            7180..7671
                     /locus_tag="IW735_001581"
     CDS             7180..7671
                     /locus_tag="IW735_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044585.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            8114..9313
                     /locus_tag="IW735_001582"
     CDS             8114..9313
                     /locus_tag="IW735_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1044586.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(9808..10566)
                     /locus_tag="IW735_001583"
     CDS             complement(9808..10566)
                     /locus_tag="IW735_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1044587.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            10734..11669
                     /gene="pfkB"
                     /locus_tag="IW735_001584"
     CDS             10734..11669
                     /gene="pfkB"
                     /locus_tag="IW735_001584"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1044588.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            11666..13111
                     /locus_tag="IW735_001585"
     CDS             11666..13111
                     /locus_tag="IW735_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1044589.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            13139..13594
                     /locus_tag="IW735_001586"
     CDS             13139..13594
                     /locus_tag="IW735_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1044590.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            13613..14587
                     /gene="lacD"
                     /locus_tag="IW735_001587"
     CDS             13613..14587
                     /gene="lacD"
                     /locus_tag="IW735_001587"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1044591.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(14698..15259)
                     /locus_tag="IW735_001588"
                     /pseudo
     CDS             complement(14698..15259)
                     /locus_tag="IW735_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            15403..16017
                     /locus_tag="IW735_001589"
     CDS             15403..16017
                     /locus_tag="IW735_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1044592.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            16258..>16638
                     /locus_tag="IW735_001590"
     CDS             16258..>16638
                     /locus_tag="IW735_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1044593.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
BASE COUNT         5220 a         2755 c         3528 g         5135 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg
      661 gaacgtttct tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat
      721 aaaggtttcc aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat
      781 tagaaacaat ctacagtagg gaagaaccat ttacacaaaa ttattgacgc tcccaaaagc
      841 attgttttta gcagtttaat attattcttt ttcaattaga ctgacttgat ttagttacgg
      901 aatagatact ttaatgtgtt gaagaagcaa cgtcccgtgt ttatttacta aagcatccaa
      961 tattctaccg aatatttaat attcacatta gccctaaaat aattgtcata ccatgttaca
     1021 tttgatataa cagcgttcat tttaaaaata gctgttagtg tgacaaaaca ttaaaccaag
     1081 tgtgacaaaa aacgtgatga aagctttccg gaaacgtatg tgaacaagta tataatgatt
     1141 ttgtcaggag gatagtcaaa atgaataaac gaattcgtga aatactcaaa ctgattatta
     1201 aaaaccctga aatgaaactt tccactttaa caagtgaact tgatttgaca cgtcgtcaaa
     1261 tcaattacgc gatcaatcaa tttaatgaag aattgaaaac aaagaacatt ccgttaattc
     1321 agagaagtca ttctggaaat attacggtac caatcgaggt tatccaaatg atatcacagc
     1381 ctgattacga cataggtgat gatcaggaac ttgtcgttct cactgatagt gaaagacaag
     1441 cattgatagt gatgactctg atcgctaata tcgattacat cagtctggat catttggtag
     1501 atgcggttga tgttagtaag acgactgtta tggaagatat caaacgaacg gaaccgttca
     1561 tcagaaatta tagtctgaca attcgatata atcggatgaa tggttaccaa ttggttggtt
     1621 cggaacaccg tatcttacag ctgttatcgg atctagtaaa acggtttccg atttttcgcc
     1681 gagaaatgat tcgcagaaaa ttggcacaag atgtttctga agaagaaatc gtccatttga
     1741 tccataacat ggaacaaatg ttgcatctta gttattctga tgagtcggta gactatttgc
     1801 agacatcagc gagattttta gtaagccgtg ggatcaagat gtcagataag cacgcgtttt
     1861 ttcccgatga tgtgacggat actcctgaat tccgaatgct gatgatcttg gtacatgaaa
     1921 caggttggag tctatccaaa agttatatgg agtggttcac gttattattt ttaacttcca
     1981 atatttttga gagcaaaact actcaaaatt atgattcgga tcaagaattg cgaaaattga
     2041 tcagccaaat ggttgaaaat ttccaaaatc aaacgttgat tttgatcgat gaccgagaag
     2101 actttgaacg taggatactg agtcatctgc gtccagcctg ttttcgaata cgctataact
     2161 tgagtttagg agtttattca atagatagtc tgatcaagga tagtaatcat gcgattttga
     2221 ttgatttgat gaaagaattg attattccta ttgagaattg gctaaataag gcctttccaa
     2281 atgatgaatt agatctttta tcctattact ttggctatca attaagtaat cataataaat
     2341 tgaataagca acgacaaagg gctgtagttg tttgtactaa tggtgtcatg gtctcaaagt
     2401 tagtacgagc caatatggaa aaactttttc cagagatcca ttttttagct tccctatctg
     2461 tgcgtgattt ctacaaattt gaagctgatt atgatctggt ctttactacg acgccactaa
     2521 aaagttctat gctgcagttt atgatcgagc caatcatgac atatcagcaa caaatcagtt
     2581 tacgatatcg agttttgagt gatttaggaa ttactaaggt tgatcgagca atggatgaat
     2641 tgttaacaat cattaggaaa tatgcaaata tcaccaatcc agtttcgttg cgagaagaac
     2701 tgcagtactt tttgatcaag gaagatggaa gcacgccgct ggacaacttc aatgtattgc
     2761 cttcattgac ttattacttg cggccgagtt atgtacaaat cgttgaagaa gagatgtctt
     2821 gggaacgagc agtggaggtg gcttgccgac cgttattgga acatcaaatc attgactcgc
     2881 gttttatcgc tgattgcttg gatcaaatca gacaaccggg atatgcaggt tacttgggaa
     2941 tgaagacttg cattccccat acaacggtcg ataaaggtgt aatcagtgac ggtgtcagca
     3001 ttttagtttg taaaaagccg atacgatttc ctaatggtga agatgtcagc ttgattttgc
     3061 cgctttcatt ttttgattta acgaagcatc tgcgagccat caatcaaatc gctgatatcg
     3121 ccaaagatga ttcattgatt gagcagttat tgattgctga tgacgaaaag acagtttatc
     3181 aactattaag acaaagatct taaggaggaa aacatgagac tagcagatta tcctaaattg
     3241 acagtgatca tgcgtggtta tacttttgat caggcagatg ctattttaca agcaatgcag
     3301 gaatttgacc atcaatttgc cgtagagatg acaatgaaca caaaaggagc tcttgaaaat
     3361 atacatgaac taaatcaacg ttatggtgac acgacttata tcggcgctgg gacggtaagg
     3421 acattagaac aagcccaagc ctgctatgag gctggtgcaa aatttttatt aggaccacat
     3481 atgttcacaa aagaaatgtt gtcttacgcc aataagaaag gattattggc ggtacctgcc
     3541 gcaatgactc cttcagaagt cgaccgtatg tttaaagaag gagctgatat tgtaaaagta
     3601 tttcctgcag cggttgttac tccacgtttt ttcaaggata ttcaagcacc tttaggtaag
     3661 ttaccactaa tgggggttgg tggaatcagt aaggagaatg cgaaagagtt ttttgaacaa
     3721 ggtgctagct atttaggtct tggttccggt atgttcaaca agcaagacat cgaagagcta
     3781 aatgttaaaa acttagcacg ttcgatgaaa gaattaattg aggctattaa ataggagtga
     3841 ggactttgga actttatttg aatgaggaac tcgtttttcg aaatgttcaa gcaaaaacag
     3901 atagtgaagt cttagcgttt ttagcaagcg agatgtacaa aaaaaactac gtgaaggagg
     3961 aatatattca agcaatccaa gaacgggaaa aagaataccc tactggatta ccatcaacac
     4021 caccaggaat cgcaattccc catgcaaact atgaaatggt caataaaaca actttagcaa
     4081 ttgcgactct taaagatccg gtcctatttc ataatatgga agataacaat gagcagttac
     4141 caatccaaat tgtgatcatg atggcgattg gggaaccaca tgggcaagtt gaaatgttac
     4201 aaaaaattgt tggaatcata caagatgagc cactgcggca agaaatgatc agagcaggca
     4261 atgatactga attattagaa ctattaaaga aagcggtttt ttaatataag gaggaaatca
     4321 taatgaaacg aattttagta gcatgcggaa atggtatcgc cacttcaaca gtagtagcaa
     4381 caaaagttag agagtatttg caacaacatg gcattgatgt ttcaacgact caaacaaaat
     4441 tgatggaagt tccaggaaaa gttcaagact atgatctatt ggtgacgact ggtcaatttg
     4501 atggtcaaac aggtggtgta ccagtaatca aagggatgcc gattttaaca ggtatcggtg
     4561 ccgatcagac aatggaagag attttgaact tactgaaata atagctgatc aaaggagctg
     4621 aaaaaatgaa aatcattatg aacgtcttta acttctttat tgatgctgga ccaacagtta
     4681 tgttgccagt catcatcacg attattggtt tgatttttgg gttaaagatt tctcgtgctt
     4741 ttaaatcagg tttgacatta ggtatcggtt ttgccgggat caagttgata ttggacttta
     4801 tgacgaccaa tgttggacca gctgccaagg cgatggttga tcgaactggt gttaaactag
     4861 atgctttgga tgttggctgg ggttcgattg cggctgttac ttgggcatcg ccaatcattc
     4921 caatcttgat ttttgccatt ctattggtca atatcgtttt gctgattttg aaacgtacac
     4981 atacgctgga tgttgatatc tggaactatc accatatggc aatcgttggt gttatagtgt
     5041 attttgtgac taagaatgtc ttcttaggtg taggtgcatc agtagtgatg gcaattgcga
     5101 cttttaagat ttcagactgg tcgcaaccga tggtagaaag tttcttcgga atccctggtg
     5161 tttctttacc aactgtttct gcgctttcat cactggttat tgcttggccg ttaaattggc
     5221 tgctggatcg tatcccgtta ttcagaaaat caaaattcac catcaaagat gcacaaaaat
     5281 atttgggatt ttttggtgat tctatgatca tggggttagt tattgggatg gtgattggtg
     5341 ttttagccgg ctatgatatc aaagcggttt tacaattagg tgtcagcatg tcagctgttt
     5401 tggttttgat tcctaagatg acttcattat ttatggaagg attgatgcca atttcagatg
     5461 ctgcacaaaa atggtcacaa aaaaaattca aaggtacacg tttatttatc ggattggatg
     5521 ctgcagttgt tgtaggtaat ccagatgtta tcacaacagc attgatcatt attccactaa
     5581 cgattgcatt agctttagta ttgccaggaa accgtgtgtt accattcgca gatttagcgg
     5641 ttgttccttt ccgcgtggca atggtcgttg ccttgactcg tggtaattta ttaaaaaata
     5701 tcattattgg cttagttgtg acagcttcat tgctatggtg tgggtcagct actgcgccaa
     5761 tgctgacagc aattgccaaa tcggttggta ttaacttagg aacttcttca atgttaatct
     5821 catcatttgc tgcgacttcc atgatccaaa gttatttggt atttatcgca tttgcttaca
     5881 aaccaatcat cggtattcca gtgttgttat tagcctttgc gggcgcatgg tattacttcg
     5941 atggtatcaa aaaagttaat catgaaatgg atgaaccatt gaaggcacaa tagtaggagg
     6001 gtttaaattg aaaatttcag atgtcacagt gtataaagta aaaccaagat ggatttttgt
     6061 taagatctcg actgacgaag gaatcgaagg ctggggagaa atgatctcag gaactaaaac
     6121 agaaacggtt gttgctggag cttatgagat aggaaaccgg ctgattggac gtaatccgtt
     6181 tgaaatcgag cgcctcttcc aagaaatgca tcgatctttc ttccgaggcg gtcctattaa
     6241 tggaaccatc gtttcgggtt tagaaatggc tctgtgggat atcaaaggaa aagcgtttaa
     6301 tgttccggtt tatgaattat tgggcggtgc agcacgtgat aaaatcaagg tttattcttg
     6361 gattggtgga gatcgaccaa gtgaagttgc agaacaagca caagatcggt ttgatcgggg
     6421 ctttacggcc gtcaagatga acgcgacaga agagctgcat tatatcgatt cctaccaaaa
     6481 agttgatgaa gtggtggaac gagttgcgtc tattcgcgaa cgttttggtg atgcaatgga
     6541 tattggtgtt gattttcatg gacgggtcca taaaccgatg gcaaaagtgc tagcaaaagc
     6601 tttggagcca ttccatccaa tgtttttgga agaagtcgtt ttacctgaaa atgaagaaca
     6661 ctttaaagaa gtagctgatt ctgtggctgt accattagct actggtgaac gcttgtacac
     6721 tcgttggcaa ttcaagaacc tctttaaaca aggtactgtt gatattatcc aaccagatgt
     6781 ggctctttgt gggggaatct tggaaacccg taaaatcgca gcgatggcgg aagcttacga
     6841 catggctgtt gcgcctcatg cgccatatgg tcctgttgca ctagcagcaa ctctgcaagt
     6901 agactcatgt acgccaaacg tctttatcca agaacaaagc ttagggatcc actataacaa
     6961 aggttttgat ctgttagatt ttgtgaagaa caaagaagtt ttccaatata aagacggcta
     7021 cgtagatcta ccttcaaaac caggtctggg cttggaaatg gatgaagaca aaatcaaaga
     7081 aattgcacaa gaaggtttga tttggaccaa tccgcaatgg aaaaattatg acggtacgat
     7141 cgctgagtgg tgataaatga aaataaagga tgatgaaaga tggcagaagc acttatgatc
     7201 acagtttttt taggactgct gattattttg caaataatcg atcaaaagaa aattatcgtg
     7261 tttgctcaga gtagtcctgt aaggaagagt ttgagtattt tactgacagt cgccatcctg
     7321 ttgattttct ggccggaaca tctgactgat cagattaaac tggttgcttt tgcttctgtc
     7381 attttgatca atggtttttt gaaggagggt ctagcaaaag attctgttgt tagatttgga
     7441 gtattgaacg gtaactttaa tcgatatcct caagtccaag tagaaaaact aggtgacgat
     7501 aaatcattta tttcatttta caaaagcaag aataattctt tttcaatgat ttttgataaa
     7561 gatcaaactg aaatgatgga tgtctttcaa gaacttgata tccattccaa aattacttta
     7621 gatcaaagtt cggaagaaaa aagagcagtt gtaaaaaaag ataatgaatg attctactac
     7681 cgaaattatg atttctttat cacgaataat gaaacttgtt ttaatgaatc atattgattg
     7741 aagtatacaa tgtatattaa gttttggaaa gaaatatatt ggtacaagat atagcaggaa
     7801 tcgattactc gatccctgct ttttttaact tcctttgcag caaaaaaagc tcgtaatcta
     7861 agagatgatg gatagttttt agaatatata tattttcttc aattccagaa aaaaatgacc
     7921 aagctagtat gctaaaaaag tacgatgctt ctatgatctt ttttccgtct ttattaacag
     7981 atgttttaat tgatcaacgc atattgtaat ctaccctaat ttccatagtt cttcaccact
     8041 tttaaaaata gtaaaacccc tacaagtatg ctatagtaaa ttgctaaaga aaaaccaata
     8101 aggtgggaga tgtgtgcgcg atcaaacaag cggaaaaatg attcgttttt attatacaag
     8161 tttgcatttt ctttattgga tgatgttttg ttctgtctat gggtttgcga cattgttttt
     8221 gatttatttc aagattgatc cagctaaaat cggagggatc ttagcattgg tcaatattgt
     8281 ttcaacggtg cttcagcctt ttgtcagtca agtgatcatc aagaaaagaa agatcccact
     8341 gatcaccgtg ctgaagacga tgatagcagg attaggagtg attctcttag ctggggtatt
     8401 gattccgaat ttgccgatca ttacttatat cttgggtggg attttagtag tttctatgca
     8461 atcatttctc aatgcgctgg catttgagta ttcaaatacc ggctacagga tcaactttgg
     8521 cttttcaaga gctggtggtt cattttctta tgctgtaact tcttttttac tgggacagct
     8581 gttagcgatt tattctgccg cgattttgcc aatcttggtt atagttgaag cagtgatatt
     8641 tttcgttatt ttgcatctgc tgccggatgt aaaaaatcaa gaagtttttc ctatgacaaa
     8701 agaacaggat cgctcagttt ctttgagaaa aaaatatcct ttcctgattt tgttattttt
     8761 aggtttcagt tttcttttta ctttccacac catgatcaac agctttttgg cacagatctt
     8821 tgaaaatatc cacggcggca gtaaagaagt tggcatcgca ttgatgatcg ccgctttatg
     8881 cgaattaccg ggaatgatct tttttgaaca gcttgtcaaa aagaaatcca gcaaattttg
     8941 gctgtctgtt gccagtattt catttgctgt cagaggattc atgatgctgc tggccagttc
     9001 gatcctgatg atggaaatga cgcatctcct acaagccttc agtttcgctt tgtttattcc
     9061 agcttcggct tatttgttca atcaatttct ggcggatgat gatcgtgtat ttggacaaac
     9121 gatgatcatc atcgccacaa ctcttggtgg ggtgatagga aatgtcttgg gcggcagtct
     9181 gctgcagcat ttcggtgtat gggagatgct gattttcggt actggttttg cggtgctagg
     9241 tgctttatta actattttgg gcattcgaaa aataccgaag acaggaaacg atagtgattc
     9301 tcttgttgag tagcataaaa atacttagat actagtttta tataaataac atatagtgat
     9361 tattggcgta attacaaaaa acgagaataa cttaagtaag catttctaaa taattgagaa
     9421 acctcatcta aaaagataag aaaatatccc taataaaact tctgtaacat tttagataac
     9481 tgattgtaat ctgaataata aactataagg agacggttag acggtctcct ttttttgcgt
     9541 attataattc tagaattgtt ggaaattgat aataattaac aagaaatatt agacaggaac
     9601 ttcgaactgc ttatcacatt cttattatta tatatttgcg taagtagtcc tggtagtctc
     9661 aatgcactat tctatcttga aactaagaag ttatagaagg agcaatgata gaaagcacga
     9721 gaatacaaat agcgattaaa tatttttcta gacaacctta gttaattcac acagatatat
     9781 tttaggtaca gtcttacaga tatagagtta gtgctgtgtg aatcctgatg ttcgcagtgc
     9841 ataggagtca agtaagattc tagccagtat agaaagtgga agtctggagc gtacctcata
     9901 atctgggaag aaggagcctt ctgatttaaa gccaacaaaa ggaatctcga tatgttccat
     9961 caaacttgaa tgacgattag cagtaagcaa aattgtcccg atctcagctt tataacagtt
    10021 ttcagctgcg gtagtcaatt cctttgtttc accgtttagt gaaacaaaga tcacgatgtc
    10081 atctttggat aattttctac taatgttttt tatgatattg ggatcagtat gcatctcaca
    10141 atatttaccg gtaagttgaa atttgaccat catttcctga gcaatcaatt ctgaaaaacc
    10201 tcgggcaaaa atcatgattc ttctagcagc cttgattcgt tgaatagcat cttcaatatt
    10261 cccgctttct accatattga tggtgcggat tacctcttgc tcatttttaa gaatagcagt
    10321 ctttattcct tcatcaactt tatcaagagc ggcaaaattg atatttgtat tttctttttc
    10381 ttttaacgaa tgtttaaaag acgtaaaccc ctcatatcct tttttcttca tagtacgaac
    10441 gacggttgct gttgaaacgt tggcatattc acttagtttt acaatggaat aggaggggat
    10501 ttcgagtatg tgactttgaa taaaatccca gagatatttc tccgattcac ttaattttgt
    10561 atccatattt tgcctccgat tagttaggat aattctattg taaagcggga gtttccttta
    10621 cttcaagttt atttaatata ttttgtaaaa gttttcaatg aatttagtca tttatgaaaa
    10681 catttacatt cgatcttgat acataatgaa tatgtaagaa aggaggagcg taaatgtcag
    10741 tttatacatg tactttaaac ttggcaatcg acctttttat cgaaacagat gaaatgaaac
    10801 catcccaagt caatcgaaca aacgatgatg atatacaagc taatggaaaa ggtgtgaatg
    10861 tttcattgat catgaagcaa ttaggtatcg atagtacagc tttaggattt aaagcaggtt
    10921 ttaccgggaa ttatattgag gattatttgc atcaaaaaga tattagcact gattttatta
    10981 gcgtaccggg aatgactaga atcaacgttt ttacacaagt taataaaacc aatgaagaat
    11041 tcaaattggt taataaagga cctgagattc ctcaggaaca aatcaaaact tttctaaatc
    11101 aaatacaaaa gctgcaagct gacgattatt tgtgtgtttc tggcagtttg ccgcgagggg
    11161 tctcacccga aattcttatt gatatcagtc gtatttgttt tgaaaataag gtccatctga
    11221 ttattgattc aagctatccg gaaataattg attgtctgcc gtatcagccg ttcttattaa
    11281 aaccaaacga ggaggaattg caatcttggt ataatgtcga gcttagtgat cgtgaaggat
    11341 atattcatta tgctgaacgc ttgattgccg atggagcgaa aaatgttctc ctttctctag
    11401 gaggagaggg aggaatattt ttaaataagg atatcgttta ttctgggaat tctcctaaag
    11461 gtaaagtagt caatacagcg tgtgctggtg acactttgtt gggaacattt cttgcaggat
    11521 atttaaaagg tgaacaggta gatattaatt tgaagaaaag tttagcagct ggaagttcaa
    11581 ccgcttttcg taaaggatta acagattttt cggatgttgc agagttggaa aaacaaatcg
    11641 aaatcaaaga gggagcgagt aaaaaatgac aacttacaaa attgttgccg caacaggatg
    11701 tccaacagga attgcccaca cttatatggc tcaagaagca ttggagcagg cagctaagcg
    11761 caaaggggta tctatcaaag ttgaaaccca tggtcaagta gggatagaga atgaattaac
    11821 accagaagaa ataaaaaacg ctgacgctgt aatcattgca gctgacaaag atgttcaagc
    11881 agaacgtttc gcaggtaagc gtgtaatcga tgtgcctgtc agcaagggga ttaaagatgc
    11941 tgaccaattg atcgaagatg cgttggctgg taaaggttca atcactgcag aaaacaaaac
    12001 agttgacgct gctggtgaga aagcggagat gggggttgca gcaggcattg gtcacagtat
    12061 ttataaaaat ctcatgaacg gtgtttctca tatgctgccg tttgtagtgg caggtggggt
    12121 aattattgca atttcgtttg ccatctgggg tgtgtactct tttgatccca atgatcctac
    12181 ttacaatccg actgccgcta tgttaaaggg tgttggtgat gcggcaatgg gaatgatggt
    12241 tcctatttta gcagcttata tcgctgaagg aatcgctaaa cgtcctggtt tggttgttgg
    12301 gtttgttggc ggactgttag ccaattctgg cggagccggt ttcttaggcg gaatttttgc
    12361 aggtttctta gcaggctact ttatcttact attgcaaaag gtcatgaaag gcttgcctaa
    12421 acaattggat ggtctgaagg cgatcttctt atatccggtg atcggtgtct tgtttgttgg
    12481 tgtagtcatg tctttattag caaatccaat ggaagcagtc aaccaaggaa tgatgaattt
    12541 cttggcagga ttccaaaatt ctaatccatt aattttaggg attatcgttg gctgtatgtc
    12601 agcatttgat atgggaggtc cagtcaataa agcggcttat gttacaggga ctgcgttgtt
    12661 ggcacaaggt aaccatacat ttatggcagg ggtttcggca gcttgtatcg cgcccccatt
    12721 gattactgga tttgccgcac tgttttttgg aaaatatttc gacacgaatg atcgcaacgc
    12781 tggtttagtc aactttatct tgggctcaac gcacattacc gaaggagcaa ttccatttgc
    12841 ggctaaagat ccgctgcgtg ttctgccaac tatgatgcta ggttcatcaa ttgctgctgt
    12901 actgacatat atgtttggtg tccaagttcc cgcacctcat ggaggttttc tagtattacc
    12961 tgttgttact ggtaaggttg catgggtagg gtcgatttta cttggttcaa tcgttggcgg
    13021 agtaatttta gggttgatcc aaaaacgtag tgtagaaaaa cgtgaagcag aagcagctgc
    13081 tgttgaaatg aaagaagcaa ccaacaacta aaggttattc tagaaagaag gatttcaaat
    13141 gactttgatc aaaacaaata aagtttttat gaataaaaat tttacgacgc aagatgaggt
    13201 aatgaacttc cttgcagatc agtcaattgt cttgagcatt agtgaagacc gagatgcagt
    13261 ttacgaaaaa ttgatggaaa gagaagaaga aagcacgact ggtatgatgg atggttttgc
    13321 catccctcat gccaaagatg ctagtatcaa ggatgccgat attgtgattg ttcggttaaa
    13381 cggtaaggtc gattggaaaa gtttagatgg agaaggaacg gattttgtaa tcgctctgtt
    13441 tattccagat tcagaggctg gaacaaccca tctaaaattt ttatctcagg tagccagact
    13501 tttgatgcac aaagatgtga cagaaggtct gaaacaagcc cagtctgctg aagaaatagc
    13561 tgaactttta aaccaaaaat tagaagaaaa ctaaaaagga agtagtgaaa tcatgccttt
    13621 aacagaaaaa aagaaaactt atattcgtca actaagtaat gaaagtggca tcatcagtgc
    13681 gttagcaatc gatcaacgtg gtgccttgaa aaaaatgatc ggtaaatttc aaaataaatc
    13741 agcaaccgat gaacaaatca tcaatttcaa aaaaatcatt tcttctgaat tgactccata
    13801 tgcttcagca atcttattag atccagaata tgggttgccg gcagccgaag ttcgcgataa
    13861 aaatgctggt ctgttgttgg cctatgaaaa aactggttac gatgcttcaa ctccaggacg
    13921 tttaccggat attttatcta tttggtctgt aaaacgtcta aaggagcaag gggccgatgc
    13981 ttgtaagttc ttgttatact acgatgtaga tgaatctgat gacatcaatg accaaaagaa
    14041 ggcgtttatg gagcggattg gttctgaatg tgttgctgaa gatttgccat ttttcttaga
    14101 attggtttct tatgatgcca acgatcctga tacttcttcg aaggaatatg ccttgaaaaa
    14161 accgcataaa gtaaatgaaa tgatgaagga attttctaag cctcgctatg gagttgacgt
    14221 tttgaaagtg gaagttcctg ttaatatgaa ctatgtggat ggttacacta aaaatgaagt
    14281 tgcttatacc aaagaagaag ctgctgctta tttcaaagaa caatccgaga ctaccaacct
    14341 gccatttatt ttcttgagcg caggagtaac tgctgaattg ttccaagaaa ccttgaaatt
    14401 tgcaaaagag gctggctcta cattcaatgg ggtactctgt gggcgcgcaa cttgggcgaa
    14461 tggtgtagaa ccattcgtta ctaaaggtga atatgctgcc aaagaatgga tgcagacaca
    14521 aggacgcaag aacatcgaag agctgaataa tgttttgaaa gaaacagcaa cagctattaa
    14581 attgtaagaa agctttaact ataaccttaa gtttgtaacc taatgatttt taataatcaa
    14641 tttgagcttt taaataaaga attggaagcg tcccgacgct tcctttttcg tttgttttta
    14701 aggcatgata ggttctatgt tgtcgttcaa taagcgcaag aaggtcagca tttttttctt
    14761 agagtatgca aaattgcttt tctacccaat gatttaaaac ggagaggcac ccttggatat
    14821 ggtagatggt gaagtaacgc atttcttctg tgatcacggt ttttttgtct tcttgtagcc
    14881 agatttttaa ataattttct tcgatcagat tatttaatct cttttgaaat tggatatctc
    14941 cgtggttgat gacgaataac ttgtatacga acgtattcgt atagacatag tcgatcaatt
    15001 gagcgtatag atcctcgtag ctatgtgtcg tatcgtagga aatgatttca tccaagtctt
    15061 ggaaaaagct attttccagt ttttcataaa gatcatagat atccgtataa tgtgtataaa
    15121 atgtcgaacg gtgaagatca gcagtttcgg tcagttcacg gatggtgatg ttttgaattt
    15181 ttttatctac taataatttt gataatgctt ctgctaacgc tttttctgtt tttttgacgc
    15241 ggcgatcctc ttttttcatt ttgcagcctc ctaatcaaca taatcaagga attgtgtcgg
    15301 ttatccgaca atttcaccgc tatctgaaca tagataaccg acaccttgtc tattataata
    15361 taagtatagc atatcaatat ttaaagaata ggagaataag tgatggaatt agcctttaag
    15421 caactggaaa agaaagacta caaaaaagcc attcaattcg cgattaaagg tatgcacttt
    15481 gattggtata ccaagaacaa actccttttg aatctttatg gaagatactt ttggtatcta
    15541 gaattgaatc gcgcgacaca aatcattgct ttatatgcgg atgatgagtt ggcaggtgtt
    15601 ttattagcag acatcaaagg aaaatcaaaa aagcatcatt catttagtca aaagttatat
    15661 gttaaactat ttgatttctt acaaaatgca tttgtgaaag acagcaaagg cgggtatgac
    15721 gacaccaatg acgaattgct ttcagactat ttaaaaaaac attccccaga tggagaaatt
    15781 gtctttttag ctgccaaccc tgatttaaag atcaagggga tcggtagtaa attattaaaa
    15841 gaatttgaac gccgagagca aggaaaagaa gtatttctgt tcacggatag cggctgtacg
    15901 tatcaatttt atgaacgacg cggtttcgaa cggaaaaaag aaaaagataa cgtcatgaag
    15961 atccttaata aagaagtaga tctccgctgt atgttatata gtaagatcat tcagtaatgt
    16021 tttttactat cagaaagcag aagtaccacg aaggtcaatg gaaaaagcga gtaattgatc
    16081 catttagatg aagataggtg tagtctcaaa gatattagct ggcaaagata tccccagtta
    16141 aaaatagatt tctgtatcca acaataaaaa tatttttgta tttattagta tatatacttt
    16201 acaaaaattt gtatatatac taattttttt gtaggatcaa tgaaatgagg ggaaaatatg
    16261 ccaaaatata aagaaatagc cttggctcta aaagagagga tcgttgaggg aaaatatcag
    16321 gaaggttcta aattacctga gcaagaaagt ctggctagag agtttaaaac aagccgtgtt
    16381 actatccgca aagctataca actccttatt gatgaaggtt tgttatatac gagacgtgga
    16441 tctggtactt ttatacgaag caatatcaga caggacaatg agaatgtcac tcaagtaaat
    16501 aatgtatttg gcacttcctc acaagaaaat acaaatgtga caagtaaaat aatccgtttt
    16561 gatgtccggt ttccgacaga ttatgaaagg cggctttctg caacctactt ccgatcccct
    16621 tcctgcaaat catgggta
//
LOCUS       DACEMS010000054        16591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEMS010000054 DACEMS010000000
VERSION     DACEMS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.055"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(45..779)
                     /locus_tag="IW735_001591"
     CDS             complement(45..779)
                     /locus_tag="IW735_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1044594.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="IW735_001592"
     CDS             1105..1515
                     /locus_tag="IW735_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1044595.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1508..2209
                     /locus_tag="IW735_001593"
     CDS             1508..2209
                     /locus_tag="IW735_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1044596.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2209..3822
                     /locus_tag="IW735_001594"
     CDS             2209..3822
                     /locus_tag="IW735_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1044597.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3812..4033
                     /locus_tag="IW735_001595"
     CDS             3812..4033
                     /locus_tag="IW735_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1044598.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4030..4791
                     /locus_tag="IW735_001596"
     CDS             4030..4791
                     /locus_tag="IW735_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1044599.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="IW735_001597"
     CDS             5172..5681
                     /locus_tag="IW735_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1044600.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5751..6290)
                     /locus_tag="IW735_001598"
     CDS             complement(5751..6290)
                     /locus_tag="IW735_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1044601.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IW735_001599"
     CDS             complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IW735_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1044602.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7357..8154
                     /locus_tag="IW735_001600"
     CDS             7357..8154
                     /locus_tag="IW735_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1044603.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8182..8817
                     /locus_tag="IW735_001601"
     CDS             8182..8817
                     /locus_tag="IW735_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1044604.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8837..9610
                     /locus_tag="IW735_001602"
     CDS             8837..9610
                     /locus_tag="IW735_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1044605.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10018..10815
                     /locus_tag="IW735_001603"
     CDS             10018..10815
                     /locus_tag="IW735_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1044606.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10793..11206
                     /locus_tag="IW735_001604"
     CDS             10793..11206
                     /locus_tag="IW735_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044607.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11190..13835
                     /gene="mutS"
                     /locus_tag="IW735_001605"
     CDS             11190..13835
                     /gene="mutS"
                     /locus_tag="IW735_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1044608.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13853..15961
                     /gene="mutL"
                     /locus_tag="IW735_001606"
     CDS             13853..15961
                     /gene="mutL"
                     /locus_tag="IW735_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1044609.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15983..16543
                     /locus_tag="IW735_001607"
     CDS             15983..16543
                     /locus_tag="IW735_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1044610.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5387 a         2964 c         3598 g         4642 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagctattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gagggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca ggaagggtat ctgtaaacga aatggaggaa
     1501 aatcaaattg actgagtttt ttgtgaatcc actatttggc ttaagtttga ctttaggagt
     1561 gtatgtatta tctgataaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg cacttgccat
     1741 ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa tggctgcgat
     1801 tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact tcttctctct
     1861 tcataaagag atggtgatct cattactacc taaatctgta acgacggcta tttctgtcga
     1921 tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg tctctacagg
     1981 tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg aaagtccagt
     2041 tgctcgcggt gtggcgttag gaagtacttc tcatgctatc ggtacagcaa aagcaatcga
     2101 gattggcgaa atagaaggaa tcatttccgg acttgcgata tgcgtaaatg ggatattgac
     2161 cgttttgttg ctgccgttat tctttcagcc ttttgctggc ttgttttgat gcgaaaacca
     2221 ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa agacgtgtat
     2281 ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat tctcctccta
     2341 gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt tgttcagctg
     2401 gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca gcagtttagc
     2461 ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt ttatgatacg
     2521 ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac gctgaaattc
     2581 cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag
     2641 cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa
     2701 acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga agaaatcatt
     2761 cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg gccatttgaa
     2821 acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt ttttaatgtt
     2881 cgtctgaacg agatcaaaaa aagacgattg gaatacatgc taggtgcctt ttttttaaga
     2941 aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat ttctgacaat
     3001 ctgttgttcc agcgattttg ccaagtcatg gagccggtat ttcctaatta ttttcaagta
     3061 gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata ttatagtgat
     3121 cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc gccctttaca
     3181 gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg gctgccagca
     3241 gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt cgcctatctt
     3301 tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa tcatttagtt
     3361 attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag acacaaacat
     3421 cccaatcatc tggcatttaa aaatcatgcg tttttgatgg gtcggtatct gactgtgttt
     3481 aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt aatgacagat
     3541 ttcccattat ttgaagagca actgttagag gagggattac ggacattttt ccgtaatgag
     3601 tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct tctgatttcg
     3661 acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt gaccgaagaa
     3721 ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca aaaaaaaact
     3781 attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt
     3841 gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct
     3901 cgtatccgat gatgaatttg atggctgtcc agtgacacct tacttattga tcaaaacaag
     3961 tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc
     4021 gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga
     4081 gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta
     4141 tttggattag atcaaaagtt cggtaaaatg gaagaactct ctttgcgatt tcctgaaact
     4201 tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa
     4261 tgccaggatc gttttggtga agttacgatt ttactgaata catcggggat attggatgcc
     4321 tacaaaccat tgatggaaac tgacgaatcg ctgtgggatc ttatttatga gacgaatgtg
     4381 aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgccg aaaaagcgga
     4441 acaatcatca acatggcttc gattgcgggt ttggttgctg gcggaggtgg gatcgcttat
     4501 acaagtgcga aacatgcaat cgtcggattt accaaacaac tcgctctgga ttacgcagga
     4561 cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct
     4621 gattttgaag ggaaaggtga gatggctgag tgggtcgcaa atgaaacacc agtcaagcga
     4681 tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc
     4741 tacatacaag gaacgatcat cccaatcgat ggcggatggt tgttaaaata accttgtgtt
     4801 tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaagaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgaccttt tgatataaac ggtgttcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gactattctt attacttgtt gcagggcagc tttttcacta ccttctgcgg cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcagt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatg gcttctcctc
     5401 ttggactgat agatgttgta gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggctgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tatggcagcg tggaagagac tttgagtctt cttccatgct gcttttttga ttaatttgta
     5761 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     5821 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     5881 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     5941 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     6001 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     6061 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     6121 acaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     6181 gtttgccccg tcaaaggaac caagccaaaa ggaatggtca tctgagaaag gacagaaata
     6241 atcgcggcaa attccgcagc aagtattaat tctttcaatg ttcggttcat aatgccctct
     6301 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     6361 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     6421 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     6481 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     6541 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     6601 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     6661 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     6721 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     6781 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     6841 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     6901 gtattctgat tgtttgccac ggctgttagg tccatgttga cctggtgcgt aagggcgacg
     6961 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     7021 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     7081 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     7141 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     7201 ctgcattatt ttacacaaac tacagtatat gatagatcat ccacgaatgt caagataatt
     7261 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gcatgtgcaa
     7321 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     7381 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     7441 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     7501 gcaatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     7561 atgttcagct ggggtctggt catgattcta tacatgaacg ctgaattagg aacatcgaat
     7621 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     7681 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttcttatt
     7741 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     7801 gtaaaactag caaaacctgc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     7861 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgccgggaa agtgatcaca
     7921 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     7981 cctgccttta cgcttgctta ctttgcttct caaggacaga cggatggaat gacagtcagc
     8041 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     8101 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     8161 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     8221 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     8281 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     8341 ttagcttttg tttattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     8401 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     8461 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     8521 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     8581 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     8641 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     8701 aaagcaattg gttgggcatt acgcgattac agtaagaccg atgccgattg ggtaaaaaga
     8761 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     8821 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     8881 agattcaaac aaacaagctt tagatagcac gagagaagca ttggaaaaat tacgcaaagc
     8941 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     9001 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     9061 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     9121 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     9181 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     9241 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     9301 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     9361 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     9421 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     9481 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     9541 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     9601 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     9661 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagcttttg
     9721 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     9781 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     9841 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     9901 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     9961 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
    10021 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
    10081 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
    10141 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
    10201 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
    10261 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
    10321 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
    10381 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
    10441 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
    10501 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
    10561 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
    10621 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta
    10681 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
    10741 ttggatgata cgacaggtca tgcaaaaaaa atccagctga ttcagatcaa cgatgatcga
    10801 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
    10861 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
    10921 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
    10981 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaac aagaagcaat
    11041 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
    11101 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
    11161 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
    11221 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
    11281 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
    11341 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
    11401 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
    11461 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
    11521 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
    11581 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
    11641 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    11701 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    11761 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    11821 ttttgacaag cgaactagtc gatccgctga aaaaagaagc aaccggcaaa ctattgactt
    11881 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    11941 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    12001 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    12061 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    12121 aagcaagaca agaaatggtc gcttctttgt tagacagcta ttttgaacgt atcgatttgc
    12181 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    12241 atgtcaatgg gcgagacttg atccagctga aaaattcttt agaacaagtg ccaatgatca
    12301 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    12361 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    12421 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    12481 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    12541 gaatcaaaaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaatcacaa
    12601 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    12661 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    12721 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    12781 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    12841 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    12901 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    12961 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    13021 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    13081 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    13141 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    13201 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    13261 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    13321 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    13381 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    13441 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    13501 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    13561 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    13621 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    13681 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    13741 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    13801 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    13861 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    13921 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    13981 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    14041 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    14101 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    14161 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    14221 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    14281 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    14341 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    14401 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    14461 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    14521 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    14581 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    14641 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    14701 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    14761 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    14821 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    14881 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    14941 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    15001 cgtagaagaa ccaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    15061 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    15121 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    15181 aaagaaagaa tttcccgttg tggataacat ggagaaaaca aatgaagaac aagcagataa
    15241 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    15301 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    15361 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    15421 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    15481 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    15541 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    15601 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    15661 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    15721 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    15781 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    15841 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    15901 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    15961 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    16021 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    16081 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    16141 aattgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    16201 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    16261 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    16321 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    16381 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    16441 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    16501 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    16561 ctgattttcg attgaaaatc catgatagcc c
//
LOCUS       DACEMS010000055        16529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEMS010000055 DACEMS010000000
VERSION     DACEMS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.056"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..417)
                     /locus_tag="IW735_001608"
     CDS             complement(142..417)
                     /locus_tag="IW735_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1044611.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /locus_tag="IW735_001609"
     CDS             complement(660..1970)
                     /locus_tag="IW735_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1044612.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW735_001610"
     CDS             complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW735_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1044613.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3516..4196)
                     /locus_tag="IW735_001611"
     CDS             complement(3516..4196)
                     /locus_tag="IW735_001611"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1044614.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4245..4880)
                     /locus_tag="IW735_001612"
     CDS             complement(4245..4880)
                     /locus_tag="IW735_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1044615.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4946..6349)
                     /locus_tag="IW735_001613"
     CDS             complement(4946..6349)
                     /locus_tag="IW735_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1044616.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6336..7367)
                     /locus_tag="IW735_001614"
     CDS             complement(6336..7367)
                     /locus_tag="IW735_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044617.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7398..7619
                     /locus_tag="IW735_001615"
     CDS             7398..7619
                     /locus_tag="IW735_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1044618.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7784..8521)
                     /locus_tag="IW735_001616"
     CDS             complement(7784..8521)
                     /locus_tag="IW735_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1044619.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8674..10014)
                     /locus_tag="IW735_001617"
     CDS             complement(8674..10014)
                     /locus_tag="IW735_001617"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1044620.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10251..11774)
                     /locus_tag="IW735_001618"
     CDS             complement(10251..11774)
                     /locus_tag="IW735_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1044621.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12152..12763)
                     /locus_tag="IW735_001619"
     CDS             complement(12152..12763)
                     /locus_tag="IW735_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1044622.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12886..13011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13093..13809)
                     /locus_tag="IW735_001620"
     CDS             complement(13093..13809)
                     /locus_tag="IW735_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1044623.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW735_001621"
     CDS             complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW735_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1044624.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14368..15165)
                     /locus_tag="IW735_001622"
     CDS             complement(14368..15165)
                     /locus_tag="IW735_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1044625.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15135..15506)
                     /locus_tag="IW735_001623"
     CDS             complement(15135..15506)
                     /locus_tag="IW735_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1044626.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW735_001624"
     CDS             complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW735_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1044627.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5509 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
     2101 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
     2161 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
     2221 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     2281 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     2341 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     2401 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     2461 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     2521 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     2581 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     2641 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     2701 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     2761 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     2821 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     2881 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     2941 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     3001 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     3061 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     3121 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     3181 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     3241 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     3301 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     3361 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     3421 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
     3481 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
     3541 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
     3601 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
     3661 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
     3721 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
     3781 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
     3841 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
     3901 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
     3961 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
     4021 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
     4081 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
     4141 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
     4201 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
     4261 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
     4321 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
     4381 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
     4441 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
     4501 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
     4561 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
     4621 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
     4681 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
     4741 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
     4801 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
     4861 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
     4921 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
     4981 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
     5041 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
     5101 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
     5161 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
     5221 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
     5281 ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
     5341 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
     5401 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
     5461 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
     5521 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
     5581 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
     5641 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
     5701 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
     5761 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
     5821 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
     5881 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
     5941 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
     6001 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
     6061 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
     6121 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
     6181 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
     6241 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
     6301 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
     6361 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
     6421 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
     6481 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
     6541 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
     6601 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
     6661 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
     6721 aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt gttttttgcc
     6781 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
     6841 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
     6901 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
     6961 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
     7021 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
     7081 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
     7141 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
     7201 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
     7261 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
     7321 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
     7381 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
     7441 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
     7501 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
     7561 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
     7621 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
     7681 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
     7741 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
     7801 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
     7861 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
     7921 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
     7981 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
     8041 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
     8101 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
     8161 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
     8221 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
     8281 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
     8341 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
     8401 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
     8461 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
     8521 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
     8581 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
     8641 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
     8701 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
     8761 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
     8821 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
     8881 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
     8941 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
     9001 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
     9061 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
     9121 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
     9181 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
     9241 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
     9301 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
     9361 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
     9421 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
     9481 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
     9541 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
     9601 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
     9661 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
     9721 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
     9781 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
     9841 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
     9901 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
     9961 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    10021 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    10081 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    10141 agaaaaaaca ctcactgaat ggagctattg acttgtccac gtgcttatcg atagccgttt
    10201 atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt
    10261 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    10321 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    10381 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    10441 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    10501 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    10561 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    10621 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    10681 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    10741 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    10801 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    10861 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    10921 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    10981 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    11041 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    11101 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    11161 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    11221 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    11281 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    11341 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    11401 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    11461 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    11521 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    11581 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    11641 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    11701 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    11761 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    11821 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    11881 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    11941 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    12001 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    12061 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    12121 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    12181 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    12241 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    12301 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    12361 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    12421 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    12481 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    12541 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    12601 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    12661 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    12721 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    12781 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    12841 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    12901 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    12961 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    13021 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    13081 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    13141 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    13201 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    13261 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    13321 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    13381 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    13441 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    13501 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    13561 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    13621 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    13681 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    13741 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    13801 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    13861 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    13921 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    13981 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    14041 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    14101 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    14161 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    14221 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    14281 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    14341 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    14401 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    14461 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    14521 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    14581 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    14641 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    14701 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    14761 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    14821 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    14881 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    14941 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    15001 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    15061 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    15121 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    15181 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    15241 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    15301 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    15361 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    15421 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    15481 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    15541 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    15601 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    15661 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    15721 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    15781 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    15841 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    15901 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    15961 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    16021 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    16081 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    16141 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    16201 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    16261 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    16321 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    16381 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    16441 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    16501 tagacactca gacggactgg tttcttttt
//
LOCUS       DACEMS010000056        15720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEMS010000056 DACEMS010000000
VERSION     DACEMS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.057"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..751
                     /locus_tag="IW735_001625"
     CDS             68..751
                     /locus_tag="IW735_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1044628.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="IW735_001626"
     CDS             753..1589
                     /locus_tag="IW735_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1044629.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="IW735_001627"
     CDS             1600..3354
                     /locus_tag="IW735_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044630.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="IW735_001628"
     CDS             complement(3630..3971)
                     /locus_tag="IW735_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1044631.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="IW735_001629"
     CDS             complement(4039..6210)
                     /locus_tag="IW735_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1044632.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="IW735_001630"
     CDS             6421..7278
                     /locus_tag="IW735_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1044633.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="IW735_001631"
     CDS             complement(7349..8206)
                     /locus_tag="IW735_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1044634.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="IW735_001632"
     CDS             complement(8191..10893)
                     /locus_tag="IW735_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1044635.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="IW735_001633"
     CDS             complement(10906..12195)
                     /locus_tag="IW735_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1044636.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="IW735_001634"
     CDS             12345..13391
                     /locus_tag="IW735_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1044637.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="IW735_001635"
     CDS             13393..15546
                     /locus_tag="IW735_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1044638.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4465 a         3287 c         2896 g         5072 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacaa agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
//
LOCUS       DACEMS010000057        15512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEMS010000057 DACEMS010000000
VERSION     DACEMS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.058"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(331..633)
                     /locus_tag="IW735_001636"
     CDS             complement(331..633)
                     /locus_tag="IW735_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044639.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(853..1219)
                     /gene="ssrA"
                     /locus_tag="IW735_001637"
     tmRNA           complement(853..1219)
                     /gene="ssrA"
                     /locus_tag="IW735_001637"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1390..2163)
                     /locus_tag="IW735_001638"
     CDS             complement(1390..2163)
                     /locus_tag="IW735_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1044640.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2307..2657
                     /locus_tag="IW735_001639"
     CDS             2307..2657
                     /locus_tag="IW735_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1044641.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2638..3354
                     /locus_tag="IW735_001640"
     CDS             2638..3354
                     /locus_tag="IW735_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1044642.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3354..4802
                     /locus_tag="IW735_001641"
     CDS             3354..4802
                     /locus_tag="IW735_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1044643.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4872..5381)
                     /locus_tag="IW735_001642"
     CDS             complement(4872..5381)
                     /locus_tag="IW735_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044644.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5371..6096)
                     /locus_tag="IW735_001643"
     CDS             complement(5371..6096)
                     /locus_tag="IW735_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044645.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6319..8439)
                     /locus_tag="IW735_001644"
     CDS             complement(6319..8439)
                     /locus_tag="IW735_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1044646.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8669..9847
                     /locus_tag="IW735_001645"
     CDS             8669..9847
                     /locus_tag="IW735_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1044647.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9863..10621
                     /gene="aroD"
                     /locus_tag="IW735_001646"
     CDS             9863..10621
                     /gene="aroD"
                     /locus_tag="IW735_001646"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1044648.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10644..11129
                     /gene="ybaK"
                     /locus_tag="IW735_001647"
     CDS             10644..11129
                     /gene="ybaK"
                     /locus_tag="IW735_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1044649.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11200..12453)
                     /locus_tag="IW735_001648"
     CDS             complement(11200..12453)
                     /locus_tag="IW735_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1044650.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12570..13919
                     /gene="gdhA"
                     /locus_tag="IW735_001649"
     CDS             12570..13919
                     /gene="gdhA"
                     /locus_tag="IW735_001649"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1044651.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14031..15377
                     /locus_tag="IW735_001650"
     CDS             14031..15377
                     /locus_tag="IW735_001650"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1044652.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         5126 a         2749 c         3125 g         4512 t
ORIGIN      
        1 gaaagacatt tgggttatat tgtgagataa taataaagaa gagtttaaag cgttccccaa
       61 aaaccacttc cccataagtg tgttacgctt taaactcttt tatatttgaa gccattaaaa
      121 agcataccat ataactgtaa aaaataatgg gaaaaagact tataattgga gtggtagtta
      181 attagtgact tatttttgat tttatagcac tgatactata aaatatagat atcatcatat
      241 tacacaatct taatactaac ttaaaaaata tcccttttat aagtatggtg ataaaatccg
      301 ttccgggcta ccttttttag gtagcctact ttaatcttta tacctttctg gatcaacgaa
      361 agtatacttc aaatagtcat aacgccgatg atcgctccgt gcgtctggca catcagtcac
      421 gatatcaaac aaaaaataca catctttctt cattctggtt tttgttgccg gaattttaaa
      481 gtagttctta ttagagtaat agagattgat taataagcta tcttcgattg ctaaaaagaa
      541 aacttctgaa tcccacacct tataaaaatc tttgacaaat ctattcgaag gatcaaattt
      601 aaaccataat tgtgtttttc cttccatcaa cataatgttc acctcaaaaa gagtatacga
      661 actaatgttc tttttgtaaa gtaggaatat cattgataat atggatggaa catatatatt
      721 aaaaagtgtt ctttttgaat gctattttga atgccatttt cattcattta acgaaaatct
      781 gcgaaaataa aaaatgctca aacccttata aaataaggat ttaagcatct agcgaaatct
      841 gctgaaatct cacaatggag acggcgggag tcgaacccgc gtccaaacac atcgccactt
      901 aaaaatctac gttcatagtc aactcattta aggattcact ttacagcttg ccgagtgaca
      961 ggcattctgt atcgctagtc tgatgttctc tttttcaact tacagacgga aagttgaaac
     1021 gtatcccact aaagttagga cccttactcg agcacatggg cgatgccgag aggatctacg
     1081 ctaactgttt ttaggcagct aaagcgtaag agttgttttc gtttttagca gttatattta
     1141 actgtaacgt ttttacgtag acgtaaccta cgaaacgcaa ttcaagctcg actgtgcctg
     1201 tcgaatccgt aacgtccccg ttagctaaca agggttacgt gtatttttca tacaaaatta
     1261 gtatagcata tattgggata aattcaataa aaaagcgaat catatcccat tatttatata
     1321 ataactgccc ctttcttaag aaaaaataag cgattttctt aagaaaagag gtagcttcaa
     1381 aaaaagatct taaatcagat caaaatactt caatgcgtgg acaattccgc cttctgtatt
     1441 tttctttgtg ataaaggtag aaagttcttt taatccgtca taggcgttgc ccatagcgat
     1501 tttgttgtcg caagcttcca acaaggcaaa atcattcgga ccatcgccaa atccaaaagt
     1561 aggaactttt tccagctgaa gttctttttg cagacgtttg acacctgccc cttttgagat
     1621 tcctttcttc actgtatcga tcgagtaagg accattccga taaaaagtta gctcaggaaa
     1681 atgttcgtga tagtattcgt cacctgattg agataagatc agcaacatat ttactgtgtt
     1741 ttctttaaag cctaaaggat caatttccgg aacatctgaa tgaatgaagt cgtaagcatt
     1801 gatcattgtt tggctatgac cggtacacca gattttctga tcgttatagt aagaaagttc
     1861 atgaccgttc aactgtacat gatcatacat tttttgacat tcttctgaag aaaatgtttg
     1921 agaatagatt tcttcgccgt ctactcgaat gaaagaacca ttcatggtga ccgcagaagt
     1981 aatgcccgca tcttccatga tagagtggat ttctgcttct gtacgtcctg tagcgatgac
     2041 ggggagaaca ttgttttctt ttaatgcgtt catagctttt gccacttcgg gtgtgatttt
     2101 tgatttcgca tctagtaatg ttccatcaag atcaaagaaa gtgattgcac gataatttga
     2161 cacaaataaa actccttttt tattggaata agtttatctt aacaaagaat gaacaaaaga
     2221 caaacctttc gtgaagaatt cctgaacata ttggttcagt caacaattta gagtatacta
     2281 taagaagaga atttaggagg gaaagaatga tttttttcag tattggtatc ttattcttaa
     2341 taattggcag tctttttcgg attctgccct caaaaggaaa tctgcctttt tatgggtatc
     2401 attcgcctct tgccgcaaaa acagacgctc attggcggct ggctcaaaag acaagcggaa
     2461 actggttttt tcttatgggc ttactgatgg cattgatcgg ctattattta aagacatctg
     2521 ggcatacgaa ttattttctg attgaaatgc ttctgctagt atttccaatt atgcctattt
     2581 ttattatgac agaaaagaaa ttacagaaat atgacctgga aactggaggg aacgataatg
     2641 aatattttaa tgattgaaga taatcaatct gtttctgaaa tgatgcagat gtttttctta
     2701 aatgaaggtt gggaagcaac tttcaagtat gatggaaaag aagggctcga tgccttttta
     2761 gaaaacccac aacattggga tatgattaca ctagacctga atttgcccac actggacggt
     2821 atgctagtag gaagagagat ccgcaaagtt tcaagtactg tgccgattat catgctgaca
     2881 gctagagatt ctgaaagcga tcaggtgatc ggcttagaga tgggggcaga tgattatgtc
     2941 acaaaacctt tcagtcctct gacgcttatt gctcgaatga aagctttgca tcgccgtagt
     3001 gagttggcag aagcaagaga agaagcagta gtttcagatg atcattttga tatcgagact
     3061 agtcatttca agatgaatac gaaaacacga gaagcgtatc tggacaaccg gccaattgaa
     3121 ggactgacgc ctaaagagtt cgatctattg tttactttag caaaaaaacc acgtcaagta
     3181 ttctctcggg agcagttgct tgaattagtc tgggattatc agtactttgg tgatgaacgg
     3241 acagtggacg cccatatcaa gaaattacga caaaaaatcg aaaaggtcgg accacaagtc
     3301 atccaaactg tttggggagt cggctacaaa ttcgatgatt ctggtgttgc ctaatgcgtt
     3361 atttgtatca gcaactctta gctttctgcg gaatggtcgc gttgatctta ttgatcgttg
     3421 gagtttcttt tacacaactg acaaaacaaa cgattgaaga aaacaattac cagcaactgt
     3481 ttggatatgc tgaatcagtt gaaaagacaa cgcaaaccta tgccgatacg ctcccgcagt
     3541 ttagtcaaga tcaggcgttt caaaatgcat tgctgctgac agagcaagta ctgacagaac
     3601 aaaatgtgaa ctttatattt atcgataaaa acgaacgtgt catttatccg actaatacag
     3661 ctttactgaa ttttacctta acagctgatc aatggaaaaa tctgaggaat ggacgtcaag
     3721 aaaaattcac atcgaataaa aatattttag gacaagcaca ggcgacctct tacgcacttg
     3781 tgccatttaa tttgaatcat gaattctatg gagcacttgt agttagtcaa cctgcgcgaa
     3841 atatcgataa cagcgttcgt ccaattacac tgaacctgtt caaaggattt attttttcca
     3901 gtatcgttgc agttattgcc agctatgctt ttgcagcatt ccaagttaag cgtatcaatc
     3961 ggttaaggaa tgcgacgaaa gaagtgacga atggtaattt tgatgtacag ctccccgtac
     4021 atgacaaaga tgaatttgat gagttagcag atgatttcaa taaaatgacg aattcattga
     4081 aagaatctca agcagaaatt gaagaacaag aaaatcgtcg aagacaattt atggcagatg
     4141 cttctcacga gatgagaaca cctcttacga caatcaacgg acttctggaa gggttagaat
     4201 acaatgcaat ccctgaaaat cagcgagaaa acgcgattaa attgatgaaa aatgaaacag
     4261 agcgtctgat ccgtttggtg aatgaaaact tggattatga aaaaatccgt acgaatcaaa
     4321 tcagtatggt cattaaaaaa ttcaatggaa cagaaacatt gaaaaatatt gttgcacagt
     4381 tagaagctaa agcagaagca gcaggagata cattgacatt gaaagccgtt gatgatattg
     4441 atgtttacgc tgattatgac cgatttgtcc aaatcatggt caatattatt caaaacgcga
     4501 tacagtttac tgaaaacggc caaatcacga ttactttaga aaaaggctat cttgaaacga
     4561 tcatcacgat tgaagatacg ggaattggaa tgtctgaaca acaaatgaaa agtatctggg
     4621 atcgttatta taaagtagat ccttcaagaa aaaatacaaa atatggtgaa tcaggattag
     4681 gtttaccaat cgtgcagcaa cttgtacgct tgcataaagg aaaattagaa gtagaaagcg
     4741 aactaggaaa aggaacaaaa ttcgctgttc gtttgcctga cgtagaaatc aaaaaagagt
     4801 aaaaaacaag cggtataaaa gatcatgaat cgatcttcta taccgcttgt ttttactata
     4861 aaatgattga tttagcccgc gggctctaaa cgatcaatat aaggactttg agcaatagta
     4921 gcatcatgag taaagagtaa gataccttga ttttcttcat gagctaaata agaaagcaag
     4981 tggagtagct cttggatctc caatacacta agttcatcaa atgtatcttc aataacaata
     5041 ttgtttcgct tggaaagcaa ggcatgaagt aattgcagct ttactttttc aaaagacgtc
     5101 aactcagctg ccgcacaatt aagcaaggaa ggagagatat caagtttttc ttgataggcc
     5161 aaacgatctt gtttgatttc tttttccggt acatccaaca ggacatggtc tttaagattc
     5221 agataaggaa acatcggcca atttttttgg atgatcgcaa aattatccag ttttgcaaca
     5281 agaacctctt ttttcaagtg ctccaatatt ttatgctgtt tttcgagagg acacaaaata
     5341 acggtcaagt gttcagcatg aatcaaatat tcaggtttca ttacgtccag gcctcctact
     5401 ttgcaaatga tggataaaaa aagtgaaagg gaatagacta agaatgagac agcatccact
     5461 gagcccaata agagtttggg caaatccctt aagtatatct gtgaaaaaaa gatcattttg
     5521 gaaatcgatg gtaaaaaaag tctgttgatc catcggaatt gtaagtccgc ttaccgaagt
     5581 caagatatca cggatatctg gcagttcagt agtctttaag acaagaaaat tgatcctttg
     5641 aagaagcgct tcataaactg actggaagct tacgatccac aataaaacca agatacaaca
     5701 aagaaagaga ggaaagaaca gctgccagaa aataaaaagg aataatttgg ttttagaaag
     5761 accagtcagg tgccaattga tcaattcctg cttgttttgc ctgactgaat agtaaaagaa
     5821 aaagataaaa aggaaggcaa atagaaaaaa taaactgaat atgcctttta tataaaatcg
     5881 gctagctaat tctaaagccg gaagaagctc tgtagatgca gaagcgccaa taaatgaaag
     5941 ccgatcttgg acttgattgg ctaaagccga ttgcaaatgg taaagagtca ggcatgctat
     6001 caataaaaaa gcagaaaagc ttgaaaataa acaatagagt attgtctgct ttttgtaata
     6061 aattaatcga gcagtagcat agcggattat tttcatactt ttcacctcta taaaaagtat
     6121 agaaaatgaa tatgaataaa gtatgaaatt cctttagtga aatcacgaat taaaaccaga
     6181 aaatattttg taaaaagcaa gaggttgtga agaagctgcc ctattctatt accgtagatg
     6241 caagcttttg aacactgttt attcgaaaag aggagcagcg acaataattt tgtcgctgct
     6301 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
     6361 cgtttgcgtt ttataaagat caacagttga ttgtccgttg tgttcactaa agacactagt
     6421 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
     6481 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
     6541 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
     6601 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
     6661 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
     6721 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
     6781 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
     6841 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
     6901 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
     6961 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
     7021 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
     7081 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
     7141 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
     7201 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     7261 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     7321 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     7381 actaatgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     7441 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     7501 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     7561 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcatgctgga gaccattctc
     7621 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     7681 attttttcct ctagcgagcc cgaacaaatc gtcatttggt gctgcataat gttcttttac
     7741 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     7801 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     7861 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     7921 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     7981 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     8041 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     8101 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     8161 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     8221 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     8281 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     8341 taaataggca aaaatcgttt tcaaccaaag taaaacgact agtaaagaaa aaaatccgag
     8401 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     8461 tcttaatcac agaagtgtcc tgaaaccatg ttacacagct cacttattct catttatttt
     8521 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     8581 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     8641 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     8701 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     8761 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     8821 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     8881 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     8941 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     9001 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     9061 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcatag gaggctatga
     9121 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     9181 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     9241 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     9301 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     9361 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     9421 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     9481 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     9541 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     9601 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     9661 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     9721 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     9781 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     9841 ccattagaag gagcgatgca gaatggttcg ggtaaaggga ttgaatatag gaagcggtag
     9901 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     9961 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
    10021 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
    10081 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
    10141 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
    10201 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
    10261 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
    10321 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
    10381 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
    10441 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggttgg
    10501 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
    10561 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
    10621 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
    10681 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
    10741 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcgt atatttaaaa
    10801 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
    10861 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
    10921 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
    10981 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
    11041 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
    11101 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
    11161 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
    11221 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
    11281 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
    11341 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
    11401 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
    11461 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
    11521 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
    11581 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
    11641 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
    11701 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
    11761 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
    11821 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
    11881 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
    11941 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
    12001 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
    12061 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
    12121 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
    12181 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
    12241 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
    12301 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
    12361 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
    12421 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
    12481 atctgaagaa agaaaacaga aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg
    12541 agggaagata agcaaaggag cgtggaacga tgacaaacgc aacagaatat gttcaagcaa
    12601 ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga atttttacaa gcaatcgatg
    12661 aattcatgcc cacggttatg tcttttttgg acactcatcc cgaatatatc gaaaagaata
    12721 tcttagaact tttaacggaa ccagaaagag ttattcaatt tcgagtgcct tggcaagatg
    12781 atgaaggaaa ctggcgagta aatagagggt accgtatcca atacaactca gctatcggcc
    12841 cttataaagg tggattgcgc ttccatccta gtgtcaatct aagtatcttg aaatttttgg
    12901 cttttgaaca gattttcaag aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg
    12961 gaagtgactt cgatcctaaa ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt
    13021 tcatgctgga attgtcaaaa catatcggcc catcgattga tgttcctgcg ggagatatcg
    13081 gtgtaggagc tagagaaatc ggctatttgt tcggcgaata taaacgattg aaaaaatatg
    13141 atactggtgt gttgacaggt aaacctttgg atttttgggg aagtaagata cgtacggaag
    13201 ctactggtta tggattagtg tattatgtca aacatctatt gaacgaagaa aaagattcat
    13261 ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt agccatttat gcaatcgaaa
    13321 aagtgcaaga gctaggcggg aaagttgtta catgttcaga ttctagcggg tacatctatg
    13381 atccagaagg aatagatgtg aaactattga aagaagtcaa agaaatcaat agacaacgtt
    13441 tgacggaata cgcaaaccaa cgtccttctg ctatttatca tgctggagaa tctgtttgga
    13501 cattgaaaga aaaagcggat atcgctttgc catgtgctac tcaaaatgaa atagacgaaa
    13561 gcttggccga aatcttagta gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc
    13621 catgtacagt aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa
    13681 aagctgctaa tgctggagga gtagcagtat ctgctttaga aatgagtcag aacgtccagc
    13741 gattgacttg ggaaagagag caagtggatg agcagctgga tcaaatcatg gaaacgattt
    13801 accatacgtg tcgccaaaca gcagccgagt acggcgacga aaaaaatctg cttcttggag
    13861 caaatgttgc tggatttgaa aaagttgcca aagcaatggc agcagaaggt ttagtttgaa
    13921 aaatgaaact ttcttggaaa tattgtcagc attttgttta tttgcttgaa aaataataaa
    13981 aaaaaacgta aagtattacc tgtagagtaa acctataagg aggagaacaa atggcacaca
    14041 ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta gggtacatgc
    14101 aaagccaagt aacagcagcc cacaacgaat tacgggaagg tactggcgca ggaagcgact
    14161 tccgcggctg gatcgattta ccaacgaact atgacaaaga agaatttgca cgtatcaaag
    14221 aagcagctaa aaaaatccaa tctgattctg aagtattagt tgttatcggt atcggtggtt
    14281 cttatttagg cgctcgtgca gcgatcgact tcttgaatca cacattcttc aatttattag
    14341 atagcaacac aagaaaagca ccacaaatct tctttgccgg aaattcaatc agctcaacat
    14401 atattgctga cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa
    14461 aatctggaac aactactgaa ccagcgattg cgttccgtgt attcaaagag cttcttgtta
    14521 agaaatatgg acaagaagaa gcaaacaaac gtatctacgc tacaacagat aaagcaaaag
    14581 gtgcagttaa agtagaagca gatgcagaag gttgggaaac tttcgtgatt cctgatgaca
    14641 tcggcggacg tttctctgta ttaactgctg ttggtttgtt accaatcgct gttagtggtg
    14701 cagatatcga tgcattgatg caaggtgccg ctgatgcaag caaagcatac tcaagcgaca
    14761 aactagaaga aaacgaagct taccaatacg cagcaatgcg caacattctt taccgtaaag
    14821 gcaaagtaac tgagttgttg atcaactatg aaccaggaat gcaatacttc tcagaatggt
    14881 ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg
    14941 caaacttctc aactgatttg cactctttag gacaattcat ccaagaagga cgtcgcaata
    15001 tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag
    15061 cagaagattt agacggtcta ggctacttgc aaggaaaaga agtggacttc gtaaatacaa
    15121 aagcatttga aggaacattg cttgctcata cagacggtga cgtgcctaac ttgctagtca
    15181 aaattccaga aatggatgct tatacattgg gttatacaat gtacttcttt gaaattgctg
    15241 taggtatttc aggttacttg aatggaatca acccattcga ccaaccaggt gtagaagctt
    15301 acaagaaaaa catgttcgca cttctaggca aaccaggctt tgaagatctt gctaaagaat
    15361 tgaacgaacg cctgtaagat aagggttttt cgctagattt aaataatcag tgatagaaac
    15421 atgttcgctt taaaatgaaa actggtaccc atttaggtac tcaaacatac tgcatcgttt
    15481 attcagaata aggggctgtg acaagacgtt tg
//
LOCUS       DACEMS010000058        15352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEMS010000058 DACEMS010000000
VERSION     DACEMS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.059"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..563)
                     /locus_tag="IW735_001651"
     CDS             complement(<1..563)
                     /locus_tag="IW735_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1044653.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IW735_001652"
     CDS             complement(750..1400)
                     /locus_tag="IW735_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044654.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW735_001653"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW735_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1044655.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IW735_001654"
     CDS             complement(2912..3910)
                     /locus_tag="IW735_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1044656.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW735_001655"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW735_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1044657.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="IW735_001656"
     CDS             complement(4926..5657)
                     /locus_tag="IW735_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1044658.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="IW735_001657"
     CDS             complement(5661..6926)
                     /locus_tag="IW735_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1044659.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW735_001658"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW735_001658"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1044660.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW735_001659"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW735_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1044661.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="IW735_001660"
     CDS             12268..13770
                     /locus_tag="IW735_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1044662.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="IW735_001661"
     CDS             13772..14509
                     /locus_tag="IW735_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044663.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="IW735_001662"
     CDS             complement(14554..14727)
                     /locus_tag="IW735_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1044664.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..>15352
                     /locus_tag="IW735_001663"
     CDS             14703..>15352
                     /locus_tag="IW735_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1044665.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAF"
BASE COUNT         4519 a         3288 c         2590 g         4955 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg aattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt tt
//
LOCUS       DACEMS010000059        15299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEMS010000059 DACEMS010000000
VERSION     DACEMS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.060"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(29..1324)
                     /gene="purB"
                     /locus_tag="IW735_001664"
     CDS             complement(29..1324)
                     /gene="purB"
                     /locus_tag="IW735_001664"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1044666.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1337..2470)
                     /gene="purK"
                     /locus_tag="IW735_001665"
     CDS             complement(1337..2470)
                     /gene="purK"
                     /locus_tag="IW735_001665"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1044667.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2454..2942)
                     /gene="purE"
                     /locus_tag="IW735_001666"
     CDS             complement(2454..2942)
                     /gene="purE"
                     /locus_tag="IW735_001666"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1044668.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3358..4665)
                     /locus_tag="IW735_001667"
     CDS             complement(3358..4665)
                     /locus_tag="IW735_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1044669.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLVKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4668..5249)
                     /locus_tag="IW735_001668"
     CDS             complement(4668..5249)
                     /locus_tag="IW735_001668"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1044670.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5365..5462)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5821..7113)
                     /locus_tag="IW735_001669"
     CDS             complement(5821..7113)
                     /locus_tag="IW735_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1044671.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7110..7733)
                     /locus_tag="IW735_001670"
     CDS             complement(7110..7733)
                     /locus_tag="IW735_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044672.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7736..8572)
                     /locus_tag="IW735_001671"
     CDS             complement(7736..8572)
                     /locus_tag="IW735_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1044673.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8589..9476)
                     /locus_tag="IW735_001672"
     CDS             complement(8589..9476)
                     /locus_tag="IW735_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1044674.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9492..10775)
                     /locus_tag="IW735_001673"
     CDS             complement(9492..10775)
                     /locus_tag="IW735_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1044675.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11458..12141)
                     /locus_tag="IW735_001674"
     CDS             complement(11458..12141)
                     /locus_tag="IW735_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1044676.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12159..12776)
                     /locus_tag="IW735_001675"
     CDS             complement(12159..12776)
                     /locus_tag="IW735_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1044677.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12786..13610)
                     /locus_tag="IW735_001676"
     CDS             complement(12786..13610)
                     /locus_tag="IW735_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1044678.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13603..14163)
                     /locus_tag="IW735_001677"
     CDS             complement(13603..14163)
                     /locus_tag="IW735_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044679.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14337..15293
                     /locus_tag="IW735_001678"
     CDS             14337..15293
                     /locus_tag="IW735_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1044680.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         4495 a         3414 c         2619 g         4771 t
ORIGIN      
        1 ttaattgaat tttgcaactc actcttcttt attccaaacc tactcgtttg aatatcacgt
       61 caacgttttt caaatgatga tgataatcaa aggcatcgtc tatttcttct ttagataata
      121 ccgatgtgat ggttggatct tcttctaaca gcgggcggaa ctgcacttgg ttgtcccaag
      181 cgtacgcagt ttttggctga acaagatcat aagcttcttc tcttgtcatt cccttatcga
      241 tcaatttcag cataacgcgc tggctataaa tcaaaccaaa tgttgcagac atattccgct
      301 tcatgttttc aggaaagacc gttagatttt taacgatatt gccaaaacga tttaacatgt
      361 aattcaataa aatcgtggta tcaggcaaga tgattcgttc tgctgaagaa tgagaaatat
      421 ctctttcatg ccataatgtg acattttcat aggcagtgac catgtgtccg cgcaccactc
      481 tggctagacc agccatattt tctgaaccga tcggattcct tttgtgcggc atagcagaag
      541 aacctttttg acctttcgca aaaaattctt ctacttcacg tgtttcggat ttttgcaatc
      601 cgcgtatctc tgttgcaaaa cgttcaatac ttgtagcaat aagcgccatc gctgagaaat
      661 attcagcatg tagatcccga gggagaactt gagtagaaat ttcttgtgga cggatcccca
      721 attgtttaca tacatactct tcaataaatg gcgggatatt tgcgaacgtt cccactgctc
      781 ctgagatttt tcctgcttct actccttttg ccgcatgttc aaatcgttcg atgttccgtt
      841 tcatttctga ataccatgtg gcaagtttca atccaaaagt agttggttca gcatggacac
      901 cgtgtgtccg acccatcatc actgtatatt tgtgttcttt tgctttttcg ccaatgattt
      961 ccgtaaaacg tttcaagtct tcccgtaaaa tatcatttgc ttgcttgatg agatagccat
     1021 aagcagtatc tacgacatcc gtacttgtta gaccataatg gacccatttt ctttcttctc
     1081 ccaatgtttc agaaaccgct cgtgtaaacg caacgacatc atggcgtgtt aatttttcga
     1141 tttccaagat ccgatcgata tcaaatgatg catgctcacg aatttttttc acatcttctt
     1201 gcgggatttc accaagttct gcccaagctt catctgctaa aatctcgact tcgagccacg
     1261 cacggtattt gttctcttcc gtccagatat tccccatttc agggcgtgta taacgttcta
     1321 acatgttttt tctcctctaa tcccaaatat tcgtttgttt gatttcttcc agcgtttgct
     1381 ggatgtcttc tgtcaagata gtgatatgtc ccatcttgcg accttttttt gcttgttttt
     1441 ttccatagaa atggaattgc cagtctggct tattctcaat caagcgatag ctttccagca
     1501 gttgttctcc caaaacattg accattactg cattgcttat caaacgtact ttttctccta
     1561 atggccaacc acaaatacct cgaatatggg catcgaactg actcatagaa caagcttcaa
     1621 tagaataatg tccagaattg tgaggacgag gagctaattc attcacatat agcccgcctg
     1681 tttttgttaa aaacatttct acccctaaaa cacctgaaag gctcactgct tctgcaatca
     1741 cacgggcaat acgctcagcc tcctcgatca catcctgatc aatcgcggct ggagcaatcg
     1801 tttcatgaag aatattgttg tggtggatgt tttccacgac cggaaaagtc gtgaaatctc
     1861 cttgaccatt tcctgccacc atgatcgaaa tctctttttc aaacgggatc catgcttcta
     1921 aaacacaggt tccttctctt aaaaggtcca tggcaggagc tagatccgcc cgacttttca
     1981 aaacatactg ccccttgcca tcatagccgc ctcgagtggt tttcaaaaca caaggatagc
     2041 cgatcccgtc aatcgcatct tggatatccg tcggactgac gatcgttgca taaggagcaa
     2101 tcacgatgtt attcgtttcc aaaaaagatt tttctaacag gcggtcttgc gtaatcgcaa
     2161 gcaagtctgt cccttgagga ataaatgaca tgggtaaaat cgcatttaac gcctctatac
     2221 tgacgttttc gaattcatag gtaatgacat ctgttcttct ggcaagctct ttcagcgcaa
     2281 aggtgtcatc atagacactt tcaatgtgcc aatctgcaac ttgtgcggct ggacaatcgc
     2341 tcacaggatc cagcacacct actttaaagc ccatttcttt tgcactaatc gccatcatac
     2401 gacctaattg accgccaccg acgatcccga tcgttgaacc aggcaacaaa ggtttaacca
     2461 agttgatcac tactttctat aacagtttta gccaacattg aacgtctttc agccaattta
     2521 gcctccaatt ctaaatcata catggaaagc atctgtatcg caagtagtcc agcattaatt
     2581 gctcccgctt ttccgattgc tgtagtagct acaggtactc ctccaggcat ttggacaatc
     2641 gaaagaagcg aatccaaacc atttaatgtt cgtgattgga ccggtacacc gataaccggc
     2701 aaagttgttt ttgatgcgac catacccggc agatgcgcgg ctccccctgc tcctgcaata
     2761 atgattttga ttcctttttt tcgagcgttt tgtgcatatt caaacatcaa atctggggtt
     2821 cgatgagcag aaacgacctt tttttcataa gacacaccga attcatccaa tatttgacat
     2881 gcttctttca ttgtttccca atctgaagta ctccccataa tcacagagat ttttgctgtc
     2941 attgtttttc ctcccagttt ttctacattc atattttaac aatcactttt tttgatgtca
     3001 aagaaatttc gaatgttaac aaagaaaaac attttattgt tcgtgtttat taaagataaa
     3061 aaaaaggaat gtgacaaaag ttaggtcaca ttccttttta tggataaacg gtgttctcct
     3121 gccaactcct cggtcacaag gaacaatatt caacaaacat gagaagcttt tttttgaaat
     3181 tgttccttgt gactcggaga tgaacggcag gctcacgacc tcttccttca acggtgttca
     3241 atcagctgac cctcggtatt agctcaaaca aacggcaaaa tacgaggaac tatttttccg
     3301 tttgtttctc taacaatcag gtcaaaacgc tgatttcaca acctttcttt tgctctatta
     3361 cttcttgaag gcttcttcca atatttcttc atcttttttc aatccattaa agatcaaatt
     3421 caatatgata gccgtaatgc tactcattac gatgccattt ccagtgaaca tttgcaatgt
     3481 ttgcggcatt tgactgaata atgtcggcgt aacattgaaa cccagcccaa atccaacaga
     3541 aacagcaaca atcaatagat ttttatcatt gctgaaatca acttccaaca gcatacggat
     3601 tccttggact gccaccatcc cgaacatgac taacattcct ccaccaagta ccggctcagg
     3661 gatgatttgt gctaacgcac cgattttagg gaataatcct aaaacaatca agaagaatgc
     3721 agagaaatag atgggttttc ttgttttgat tcccgaaagt tgaaccaatc cgacattttg
     3781 agaaaagcca gtataaggga atgtattgaa aataccgcct aagataacgg caagtccttc
     3841 tgcacgatat ccctttttca attcttcttc tccaacattt ttcccagtaa tgtcaccaag
     3901 tgcaaaatat acacctgttg attctaccat gctgaccatg gaaatgatga tcattagtaa
     3961 aatcgaccat acgtcgaaag ttggtttacc aaaataaaag ggttgtggaa catggaacaa
     4021 cggcgcatct ccaatcgcgc tcagatcaat cattcccatg aaagcagcta agatgcttcc
     4081 tccgactaaa ccaatcaata ccgcgattga acgaatgaac ccttttacca gcatttgcac
     4141 gcctacaatt aggagaatcg tcacaaatgc aagtagcaga tttgttttgt caccaaaatc
     4201 agctgctgtt gcattacctc cacccatttt ctcgattgct acaggaatca aagtcaaacc
     4261 aataacggtg ataaccgttc ctgttactat aggagggaat agttttttaa ttttagaaaa
     4321 gacaccagaa actaacacaa caaagatccc agaagcgata atagatccgt agatagctcc
     4381 tactccttca ttggttccga tcaagatcag tggagctact gcttggatcg cacatcctaa
     4441 aacgacaggc aagccaattc cgaaaaatcg gttaactgtc aattgcaata atgtcgctaa
     4501 tccacacata aagatatcaa ttgaaatcaa ataagtcatc tgttcttgat taaagcctag
     4561 tcctgtaccg atcaaaagag gcaccgctac tgctcctgca tacatcgcta gcagatgctg
     4621 cagccctaaa accgcagctt ttccattttg tgtttctttt tccacgatta tgcgtcctcc
     4681 tcaagaaact caacttgtcc attcgtcaag gaagcaatac gcgcaagaga aacgactctc
     4741 aatcccattt cttcaagtaa ctgacgtcca tcttggaaag acttttcgat gacgatcccg
     4801 attccttcta cttgcgctcc agcttgctgg cataattcga tcaatccttt tgccgcttgt
     4861 ccgtttgcca agaaatcgtc gataatcaat actttgtctt ctgaagataa aaatttacga
     4921 gaaatagaaa tcgtacttgt cacttgtttc gtaaaagaat aaacagaagc tgtcaataat
     4981 tcttcatcca tcgtcaaact ttttgctttt ctagcgaaga tcattggaac atctaattct
     5041 cttgcagcaa ataaagctgg tgcgatccca gaagcttcaa tggtcacaac tttcgtgatt
     5101 ccttgttctt taaatacttc tgcaaaccgt tttcccattt gttccatcaa tacagggtct
     5161 acttggtgag tgacgaagct gtcgactttt aatacgccct cgcctaatat acgtccgtct
     5221 ttttgaatac gttcaactaa ttctttcact ttatttgctc ctttatcatt tattaatacc
     5281 gaaataaccg cttttgtgca aaaacctact atgtttctgt taaagaaaaa caccctccac
     5341 tttatcataa gaaaagaagg gtgaaatagc caatctttta cttatagtcc agcatttacg
     5401 gtactggcta gaaactgtca tccatattat gacgatatat aagtaggtat gtaaaactaa
     5461 taatagtgta aattcatttt cttgcaactg ttttgcacgt gaaggcagtt tgccatattt
     5521 gaataaaaaa tgttgtttca aatattgaaa acagtctaac actactttct gaaactgaca
     5581 acgtggttta cttatttttt tgtaaaatct aatgattttt aatgcattat tcctttttgt
     5641 tcgggtttaa atagttattt ttattttctc ctatctaaaa aagcagccca cataaaaaga
     5701 aggcatgaca aaaagcatgt catagcccct ttttccgaat aaatagtgtt caaaagacag
     5761 cctatcggca ataaggcaag attttataaa ctacgggaag taatttccgt aaatctattc
     5821 ttattctttt aagctgacaa gctcgtcaat cactactgct tttcctgaag caatagaatg
     5881 atttgctgcg attcctgtca aaatcgacaa aataccatct acgtgagttg ctgcccgatt
     5941 ccacggatct tcttcttttt cttcaccaaa tagatcatct aataaacgcg gatctcctcc
     6001 gccatgtccg cccgcttctt gttcgacgat tacctcatat ggctcaccga acatcggata
     6061 gacatggatg gactgaagaa cggctgctcc ttcatccttt ttatctccac ctgcattgat
     6121 gtaagacttt tcgatcactt tcatttcgat ccgtcctttg ctgccattga aagaaacatt
     6181 gaatccttcc cacggcatat acgcgtttag cgaatacgtc aaaatcgcac cagtttcata
     6241 tttgaccgtt acgcctagtg tatcttcaat agagatatca tcggaaaata cactgcggtc
     6301 acgaatatat ccactgtctt tttctgcttc taaatacatt tctcgcaatt cctcattggc
     6361 tgacaggtct atagcaaatg gatcagcttt agctagttcg cttccatagc atcgactgta
     6421 aaatatttct actccgcgtt tctccgcatt atgcatccca taaaagttta atccaccgaa
     6481 tgcaaatacc ttttggggac ggctgttcaa ccaaaaattc actagatcaa aatgatgggt
     6541 agatttatga acaagaagcc cgccgctatt gtgcttattt cgatgccatc ttctgaaata
     6601 atctgccccg tgttgcgtat ttaacagcca ttcgaagtgg atagaaaaca catcaccaat
     6661 cacatcactt tctatcagtt cctttatctt cgtgtgatac ggtgcataac gataattaaa
     6721 agtgactcgc aggcttcttc ccgtctgttt ttggaccgtt attatttctt gagctttttc
     6781 ctcatcgata gtcagaggtt tttcgcaaat aacatcatat ccttttttca ttgcacgaat
     6841 gatataatca tgatgcgttc gatctactga cgtaataatg acttcagccg gccgtacgtc
     6901 atccagtagt tgctcaaatt gtgatggcat acatgtcttt acttttttct gtccatactt
     6961 gttcacaagt atccgatttg cataatccat ccttgtttga gatacatcac aaaaaccgac
     7021 tagttcacaa gtttgatgat acgtagaggc aattgcctca taaaaaaaac gcgctctccc
     7081 accaagtccg acttgtacat attttttctt catttaaagt cattctcctt ttcttttaag
     7141 caaaatactg tgacaaatgg cagccctgga tacagtaatg cagtgagtaa aaaatcatgc
     7201 tgtatacacc agaacagtcc atacaaaagg cataaatacc acagaaaccc cttgacgttt
     7261 tttgacaaca gccagatatt ccttttgatc aaataaacaa gactatctgc tttatcttct
     7321 ttgcagatat caattagcgt actaaaaaaa ctatgggata caaaaagcaa cagacacaat
     7381 agaaaaggaa tcagatacca agctccaatc tttcccacta ataaatacca atcaactgca
     7441 aaaataatga caaacagctg aaacaacgta atgatgaaac acatccgttt gatttttcct
     7501 ttcctccaac ataccttttt ttctgccaag taatggacag atagataata agaaggatac
     7561 atcatcaaaa aataaatcag atagacaaca cgagcagaca gcactaatcc taaagtgaac
     7621 aaacatccta gaactaaagc gaaaagagca ttactgataa gactggttaa aaaaatccga
     7681 cagacatagt agttgagctt gtaaagaaac atctcttcta ttggcttttt catattcagc
     7741 ccttcagacc gcttgtactg atcccctgga caagatattt attgaagaat aagaaaatca
     7801 gaaaaaccgg tagcagcgac aacgtagaca tcgcaaacat actcgcgtaa gtcgtactag
     7861 atccagcgtc tgcaaataat ttgatggcaa tcgataccgt gtaagatctc ggcttgttta
     7921 aataaatcaa tggtcccatg aagtcgtccc atttccaata aaactggatg atgatcgtcg
     7981 tgatgatcga aggtttcagc aaaggaaaaa taattttggt aaagacacta tacttgctgc
     8041 atccgtcaat aatcgctgat tcatctaact ctttggggat gctgaccatg aattgcatga
     8101 tctggtaaat gaaaaatgct tgtccaaaaa aatgtggtaa aatcagcggc acatacgtcc
     8161 caaccaaatt cagccgattg taaataataa actgcggaat caaaatcact tgtgcaggaa
     8221 tcatcatcgt tactaacatg acgacgaacc agaatctttt tcctttgaaa tcgattctcg
     8281 ataatgcgta agcaatacaa gtcgagctca gaactgtccc tatcgtcgaa actgcagaga
     8341 tgacaaacga attcctaaag aaagtaaaaa atgaaattcc agcaaacccc gcccatcctt
     8401 ttgcgtaatt ttcccattga aaagtctctg gaatcaaatt cagcgaacca ctcataatct
     8461 cttgatccgt tttaaaagaa cctgcgatca tccataagac cggataaatc atgactagac
     8521 ctaatagcaa aataaaaccg tattgaaacc atttcgttac tgtattattc atttcttcct
     8581 cctctcattc acacaacgac ccattttttt gatgatttga aaatcagaaa ggtgacagct
     8641 cccataatca acagcatgat ccacgaaata gcactagcat accccataga attaaaagaa
     8701 aaagcttggt tatacacata aagagcataa aagttcgtgg catcattcgg accaccttgg
     8761 gtaatgataa atgcctgcgt aaaagtcata aacgccgcga ttgtctgcat aaccaagtta
     8821 tacaaaatga tcggcgaaag aagaggtaac gtgatcttgt aaaacgtctg ccatttgttc
     8881 gctccatcga tctctgccgc ttcataatac gagcctggga tctctttgat acctgcagca
     8941 aagatgatca ttgccgaacc gaattgccaa gcagacatca aaacaagagg gatcatgacc
     9001 agtttttgat cgctaaacca ggaaagcgca ggtgcgccta atcgttccag gaaaatattc
     9061 actaacccgt ttttagaaaa caactcccgc caaactagag cgatagaaat actgccaccg
     9121 atcaaactag gcacataata caaagaacga aagaaagtcg ttcctttgat attcctcgtt
     9181 aaaagataag caatcaatag accaaagatc agtttcacag gtacagatgc aaatacatag
     9241 agaaaagtga ctcggatgga gttcaaaaaa cgggcatcac tcagcatctt tatataatta
     9301 tccaagccga tccatgagac tggatttccc aaacgataat cggtgaacga ataataaagt
     9361 gacaagatca tcggtatcac tgttatcaat agaaatccta ggatgaatgg agaagcaaat
     9421 acgtaaccga ccacattgtc ttgatccaag aaccgtttta gtttttcttt tttcatgcct
     9481 tcctcctgct gctatcctag aatgtttgac gcaaactcat aaagactctc tgctgcttct
     9541 tctgctgtta cttcttcata aatgactttt tcaactaaaa gattgtattg gtcttgaatt
     9601 tccggattca aagcactatc aaagacattc acttcgccgt tgtccatatc ggaaatcatt
     9661 tctaaataat caaatgtcgc ttgcaaagtt gcatcatctg cacgagcgag ctgttctcga
     9721 atattcgaca tgattggtac accacgttct ccttttaaaa tttcattggc ttcttcactg
     9781 ttttgaaaga aatcaataaa cttagcagct tcttctttat gggctgagct ttttgccata
     9841 ctcagcattt gcgaagaacg taaagggata ccagattctc catctgcttt tctgcgaggt
     9901 ggattaatca actgaatctc catatcttca ggagcccctg acaatatttc tgtcaactgg
     9961 ttacttgata gaaacaccat tgccgcttct ctatccctaa ccgcctgaaa agacagatcc
    10021 gtcgtacttg cagctccacc acctggttct ggatgggccc ctttgtctac cagattttta
    10081 cgcagctgga aaaaatctgt tagatagtct ggattttcaa atcccaagct gttagataga
    10141 tcttcccgat tataaaaatt caatcctttc tcaacttgtg gtatttgaat aatacagcca
    10201 gctataaaat cttcgaatct tgaaatagca tagattccta attcttcagt gatttgatag
    10261 ctgatttgtt caaattcatc ccatgtccaa tttgaagctg gttcagggat atttaattgt
    10321 tcaaatatat ttttattgta agcaatcgca aacgaattca ccccgttaga cagtccaact
    10381 aattgcccgt cgtaagtcgt cgttgctaaa taggattcat ctgcattaga aatatcaatt
    10441 atcccttgct ccacatattc atctaatggt tcaatctgat tgatgtattg aggaaattga
    10501 ttgttcagtt ggaaaatgtc ccagatatca tctgatgctg ctagtgtacc taattttgtg
    10561 taatatcctt cataatcata aaattcataa tcgatcgtca catggggatt tttttcttca
    10621 taaagctcga ttacttttac tgttgcatcg tgtcggactt gcgaacccca ccaagccatg
    10681 ttcagcgtga tttttttgtc atcttcttgc ccacaagccg tcaagaagaa taacataaca
    10741 cttaaagcta agatgattct cattcttttc ttcattctct gcacctctac ttccattgtt
    10801 atttttcaat atactgtatt tttatacaga acatatttat taaacgatct ttttcaaggg
    10861 gaataaaaac caagacttgc cttatcgttc tgcctaggat gaacaactca atactttcaa
    10921 gaaaaattag atagtgctga ttcttctttc atcgctaaaa acacttcttg cttcaatgat
    10981 gggaggataa ttacgcaaag aaaaaaggat atcagacaac tgcccaatat ccttgacata
    11041 gctaaataag catgcttctt cgtgtgcgct cttccctaaa aaacagaaaa ataactagta
    11101 attctgtatg aaattaggta caatatacac gtcgatgcac aagcattgac tatggcaact
    11161 tgattggtca gttcccgctg acttttcaag ccccgacaat ttagctgcta ctaaattgtc
    11221 gggtgccttt tttagttttt attcgttttt cattctatag aaagcgcttc aataaatcaa
    11281 gacatttttt tgttagaatg ctaataataa caaaaggttt taactcttct tatgtacatt
    11341 acaggtaaaa aaacaaaaaa atttctcttt tttttgaatt ctgaacaaat agtactggag
    11401 tacaaaagac gaggaagagc cactatctgc tcttcctcgt cttttgattc tcacctgtta
    11461 ttcatgattc tttttctcag tttcccgata tttttccatt ttacgactaa aaagctctcc
    11521 gctacttggt ggcgtatatg tgatggcatt tgctcctgct tgaatggtct cagcaatact
    11581 ttcttccgtt ggaccacctg tcgcaatgat aggaagttca ggatattttt tacgaaaatg
    11641 gcgtactgct tctgcagttt ctttacctgc actgatattg ataatcgaaa taccagctgc
    11701 taacttttca tctatttctg tatatttcga cgttaccgta ctgataactg gaatatcaac
    11761 tactcgacaa acttctttta ctgtctctaa aggcgtagga ccgttcaaaa cgacccctaa
    11821 cgaaccttgc gcttctgcga acatgctcat gtaggaagaa cgagcacctt gtgtcaaacc
    11881 accacctaca ccggagaaaa ctggaatgtc agctgcctca ataatgcttt tagtaatcgc
    11941 tggatgaggt gtaaaaggat acacagcaat tactgcattc gcatttgtat tacgtatgat
    12001 cgcaatatcc gtagtaaaga tgatcgaacg gatcaatttt ccaaatatct tgatcccgct
    12061 tgcttctaaa atgatttccg gtactcttac gatatctttt ctcaattccg tcatgatttc
    12121 tggcgtaaat tgttctgcca tcagccactt ctcctttgct atctccggtc atactgattc
    12181 aattgatacg tctcgatgac ctggatgatt ttttgcgcat aaccggggtc tgtcgcataa
    12241 cctgcatctt gcaatgcttg tgcggcttcc tgatagttgg tggccgtaat cactttttca
    12301 tacagcgcag gattccaatc tactccttgg acgaacagtt gtgtatggtc atccatggac
    12361 tgttcccatg aatcatacac tttgaagtct ccttggatag ttatccattc ttcgttaaca
    12421 aattcctttg tttccaggct tactttcttc tgatccccat aagccttgat cccgaaaaga
    12481 ttattgtatt tgctagccaa ggtacttttt ccccaatttg attctaatat agcttgtcct
    12541 aaaatgatac taggaagcac tccataacca tcttgtagtt cttgggcatg cggaacaagc
    12601 cgttcaataa attgctgatg ggattgcgct tcattttctt gctgattatc agtaaaagga
    12661 gaagacagat tcttcaagga aaaaacaaag gctaagagta tcactgctac accggcaacc
    12721 actgctggcc aacggatatt cttgcgcttt tgctttttcc actttttttt cgccatgatc
    12781 aattcctact tttcatttaa ttcattcaga tattcttcta gcgtcaattg gtgtttggtg
    12841 atatactcag cgttttcttt accgacataa cgtaagtgcc aaggttcata tgtgatctta
    12901 gttatatctt cgtgtccttt tggataacga acgataaatc catactgcgc agcattatct
    12961 gcgatccatt tagctccagg ctgatctccg taacttgcat ccaatacagt agaaggataa
    13021 ctgttgtacc agttttgatc gacgacatcg actgccagac ctgtgtggtg ttcgctgtat
    13081 cctggctccg taatcgtttc tttagtaatc tttgtcgcct cctcttctga tacaccttga
    13141 cgactcatca catcttggac attttgttcg aaaacttgtt gttgggatga tacagaccgg
    13201 taagctgaga ccatcactaa tggaaatcca gctgcttcag cagccttggc aaatgcttca
    13261 tattctggag caatgcgttt gtcgatcaag tatccgttac ttaatgatgc caattgagta
    13321 gactcatcaa tttctttttc taatttgtga tctggaccaa ccaaaactag cttccaatcg
    13381 tctgttttaa catctggcaa ttgtttggac gtacgatctt ttgtttcttt gtcctttacc
    13441 ttgctgctgg ttgtttgact gttcttttct ttactagcag ttgttttggt gcttgttcgt
    13501 tcgactgctt gattgccggc ttgaacatgc gaatcatcat cgctaaaaat aacatagccc
    13561 attgctgcga tcataatcac aactgctcca aatcctaaaa atttattcac ttgaaaaatc
    13621 ctttcgttgt tcttcacgtt ccccattcgc ctcatgaacg taagtttctt ccagattttc
    13681 attcacccaa tcgagaagtt cgattttttc tccttcgatc tgtttttgaa catctgctgg
    13741 cagacgaaaa ttcaaaatat catcatagcc ccattcatca tattgaatac ttactttcaa
    13801 gtcaaggtat ctaccagcct gctgattatt tttcaaaggg atcagctcga cttttgctgc
    13861 tttgctgcta agccattttt gcgcgatgcg tgttttgatt tgcttgtact ctgcaacggc
    13921 aacgtttcgt ttttcgggaa aaataatcgt ttgacgcaaa atcttttgcg ttagcatcca
    13981 ttcataatca gtgaaaagat tttttacttt ctgcatcgct tcagatggca gatttttctc
    14041 gccatctttc catttttgcc attgttctgg actgatcagc aagtattcat ctaaaaaatt
    14101 tttttcagtt tcaaactgtt ctaataatac actcataata agatcaatat atacttgatg
    14161 cacgtttatc ccctcctttt aactattcta cttaagtttt gatcaaaaag cacctcttaa
    14221 atcgctgctt tcattttttt taatgtttca agtcctctcc tctttattct tcgtgatttt
    14281 tgttataata atagacagac aagaaactca atccaactat gaaggagcgt tttcagatga
    14341 aaaaatatca atggggaatc gttggtcttg gtaccatcgc acatgagttt gccgaaagct
    14401 tcaatcaaga gacgagcgaa ctaacagcag ttgcttccag aacttcagaa aaagcggaaa
    14461 acttcgctca ccgttacaat attccaaaag cttacggaag ctatcaagaa atgttagacg
    14521 atgctgaaat cgacatcgtg tacattgctg taccaaatag acagcatatc gaccatatct
    14581 tggcagcatt ggaagcaggc aaacacgttc tatgtgaaaa agccatcacg atgaataaaa
    14641 aagaactggc tgacgctatg cgcttagcag aagaaaagaa tttgatctta gcagaagcga
    14701 tgacaatctt caacatgcca ctttatcagc agttacgctc aataatggat acaggaaaac
    14761 tgggggcttt aaaaatgatc caagcacctt tcggcagtta caaagaacca gaccctaaaa
    14821 accggttctt caatccagaa ttagctggag gagctctgtt ggatatcggt acgtatgctg
    14881 tatctttcgc tcgtttcttt ttgagctctc aacctgaagt agttgcctct acaatggttc
    14941 cttttgaaac aggcgtggac gaacaatccg tcactatctt gcgtaacaaa gaaaatgaac
    15001 ttgctgctgt cagcttgact tttcaggcaa aaatgccaaa agtcggtatc gttgcctttg
    15061 agaacggtta tgtgacgatt gctgattacc cgcgtgctga tcgtgcggag atcttattta
    15121 cagatggtac aaaggaattc atcgaaagcg ggaatacatc tgaagcaatg aattacgaaa
    15181 taaagaatat ggtacacatg attgaaggtg acttgcccaa tcgctctctc ttcttgacaa
    15241 aagatgtcat cgaaatcttg gatcaaatgc aagttctatg gaaaaatatg taattttaa
//
LOCUS       DACEMS010000060        15095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEMS010000060 DACEMS010000000
VERSION     DACEMS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.061"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IW735_001679"
     CDS             complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IW735_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1044681.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(321..890)
                     /locus_tag="IW735_001680"
     CDS             complement(321..890)
                     /locus_tag="IW735_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1044682.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1076..1978
                     /locus_tag="IW735_001681"
     CDS             1076..1978
                     /locus_tag="IW735_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1044683.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IW735_001682"
     CDS             complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IW735_001682"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1044684.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IW735_001683"
     CDS             complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IW735_001683"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1044685.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IW735_001684"
     CDS             complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IW735_001684"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1044686.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8546..8731
                     /locus_tag="IW735_001685"
     CDS             8546..8731
                     /locus_tag="IW735_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1044687.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8793..9989)
                     /locus_tag="IW735_001686"
     CDS             complement(8793..9989)
                     /locus_tag="IW735_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1044688.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10159..11595)
                     /locus_tag="IW735_001687"
     CDS             complement(10159..11595)
                     /locus_tag="IW735_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1044689.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11888..12697
                     /locus_tag="IW735_001688"
     CDS             11888..12697
                     /locus_tag="IW735_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1044690.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12883..15036)
                     /locus_tag="IW735_001689"
     CDS             complement(12883..15036)
                     /locus_tag="IW735_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1044691.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4408 a         3201 c         2665 g         4821 t
ORIGIN      
        1 ttttttattt accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt
       61 ttacattttc taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag
      121 taaccacaat ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg
      181 tttaaccctt tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat
      241 gttcttctag ctggtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt
      301 agacacaata tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt
      361 tcagatagtt ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc
      421 tggtattctt cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct
      481 tcctcttctt ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac
      541 tctgctaaat tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca
      601 tctctacttt ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc
      661 agttcaacag gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa
      721 tgaacgatat agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca
      781 tcaagtatta aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa
      841 gttaacggtg tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac
      901 tcctaagcaa caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg
      961 acaccttgca gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc
     1021 atcctcagaa tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa
     1081 cacgaaagat atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc
     1141 taattatttc cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga
     1201 gtttgacacc gtacttatta caagagatcg atcgcatcac gaattaatcg tcacgccttc
     1261 tggaaagcag ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc
     1321 tgaaatcact cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa
     1381 tgaatacttt cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga
     1441 gattaccgat ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt
     1501 agctgtatta ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt
     1561 ggacaaacgt ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt
     1621 ctctttcaaa gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc
     1681 cgcagcattc actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag
     1741 caatgacttg acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc
     1801 agagattgct gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca
     1861 gcctcgcttc ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga
     1921 acaagtagtc caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa
     1981 gaagagctgt ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa
     2041 acgatgccgc gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg
     2101 cctttagcac caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa
     2161 gtcaatccgc cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct
     2221 ttgtcagttg atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca
     2281 gcagatgaag caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct
     2341 aataatttag aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact
     2401 ggtacaccag cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct
     2461 tttttagctg ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg
     2521 taaacgcccc agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag
     2581 atatctgcat ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca
     2641 gcaacgattg ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt
     2701 tcagttacat ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca
     2761 ttttcagctt cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg
     2821 tttgcagatt caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg
     2881 tcagaagctt ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta
     2941 gcagtgataa ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt
     3001 ggtactagtt cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca
     3061 gaaactttga tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag
     3121 atttgaacat gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt
     3181 gctttacgta caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata
     3241 tcgtcagcat cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc
     3301 cctttacgtg atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct
     3361 tcgtcttttt caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca
     3421 acgtgaacat cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca
     3481 tgttcaggat ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca
     3541 cgaccaaaat cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta
     3601 tctaaaagaa taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata
     3661 cgtgataatt gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca
     3721 gattcaatca attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg
     3781 attttggttt ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac
     3841 acagcttaaa tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata
     3901 tagtgataag tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac
     3961 gatttcgttg tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac
     4021 agcgtaagca ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg
     4081 gacaacgtgt cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc
     4141 agcgaattca tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc
     4201 gcgatcgcgg ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg
     4261 gatgatgatt tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt
     4321 gcgtcccatt acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg
     4381 gtcaattgat tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc
     4441 aggaatatca ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa
     4501 agccattgcg ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa
     4561 ttttttcaat tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg
     4621 tgcagaatac aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag
     4681 acgacgaata tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag
     4741 tccttcgtaa atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc
     4801 tccgccactg gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact
     4861 ttatcctaag tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta
     4921 acattatttt tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa
     4981 gtagtatttt ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata
     5041 agttgcccct ttatactctt gctgtcatcc acccaataag catctgacag aactactttt
     5101 cgttgcgttt tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc
     5161 acttgtaatt gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa
     5221 gtttgtgaag gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt
     5281 tgcagttcct gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca
     5341 acggcaaaca aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct
     5401 ccactactgt cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct
     5461 ctaacttcct tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc
     5521 agctgaactt gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa
     5581 taaataccta agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact
     5641 tcttcttctt ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata
     5701 ttttggattt ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca
     5761 aaaactccta ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc
     5821 cgtatgagga attgatccac tgactggaac gggccatttt gttttctttc gtttaatata
     5881 tcttctacaa aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc
     5941 ttattgacca actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt
     6001 ttatttttac gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa
     6061 ttcatcaggc tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat
     6121 cccacgaatg aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg
     6181 atatattcat atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat
     6241 acaaaatgcg acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc
     6301 agaatatctg actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt
     6361 tcctgataga cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg
     6421 tagcgaatgg cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg
     6481 cctggtcgat acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt
     6541 cgtaaaacgt tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt
     6601 tgaaacaaga gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac
     6661 ccttctactc gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct
     6721 aaaaagtcca ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga
     6781 aaagcttcat cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata
     6841 actacacctg ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt
     6901 ttgaataaaa gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt
     6961 ttataagctt gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt
     7021 gcttcacttt gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc
     7081 attgtgccaa atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa
     7141 ttcaatactt cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag
     7201 cgttcggggt ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg
     7261 atttctaaca cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg
     7321 attttgtttc tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat
     7381 cccatttgat ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag
     7441 tcagtgattc ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt
     7501 tttccattag gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa
     7561 atcgattcag ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat
     7621 cgtttgatca tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca
     7681 gataaatttt ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca
     7741 aaaagttctt cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct
     7801 tttatcacat actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata
     7861 taaaaatttc tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc
     7921 tctttattga aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg
     7981 gtttctgtca tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc
     8041 atcagctgct gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct
     8101 tcataagtat aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata
     8161 cgatagaatt caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag
     8221 ccttgttttt ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa
     8281 taagaagtaa ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt
     8341 aaaattcact agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga
     8401 agttgattat tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag
     8461 aactcaaatt ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa
     8521 ttaagataga aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt
     8581 tctaggtcaa gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac
     8641 tttgacaaca atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc
     8701 gccaagaaaa gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca
     8761 agctcttttt tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga
     8821 aagttgaata aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca
     8881 gcactgtaat tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca
     8941 aagttgctaa acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc
     9001 ttactgatca gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag
     9061 cgaagtatac ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat
     9121 gataaaatga gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct
     9181 agtttcggtg aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca
     9241 aaaaggatat tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg
     9301 atcgtagtga ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag
     9361 agatgatcca tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga
     9421 tggaaatctt ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct
     9481 gtgatcaaga atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt
     9541 ccgatcagat ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt
     9601 tctctaggtg cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc
     9661 ccattccaaa accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc
     9721 attgttaatg tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca
     9781 gctagatttc cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct
     9841 agtcccgaaa acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt
     9901 atgaatggca taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc
     9961 actaaatttt ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata
    10021 cctattttcg aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg
    10081 tcattttata tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt
    10141 ccaatccctt ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat
    10201 atcaacgatc cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat
    10261 tgtatagaaa ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa
    10321 gatcgatgca aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa
    10381 agaagccatc atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac
    10441 aatggcgatc agtggagaga ataaactact taatgcgaat agcactgcca ctactactga
    10501 agtcaaacca gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt
    10561 attcgatgta ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt
    10621 atccattttt gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa
    10681 aatacctgtg cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa
    10741 actgaatgcg atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga
    10801 ttgcatgcct tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt
    10861 acctaacgaa ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc
    10921 aaggactgtg gtcactacga taccgataag aatagcccca cggacatttt ttacaactaa
    10981 aatggctgtc agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc
    11041 taacgcaggg acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac
    11101 agagctcgaa atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc
    11161 gacatacgca acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat
    11221 cgcccgaata atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca
    11281 aataaacacc attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa
    11341 agtgaaaaat gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa
    11401 tcccattacc agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa
    11461 gggcatacct gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa
    11521 gaatgtcgtt actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc
    11581 gaaaaacttt tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat
    11641 aataaaaaat aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt
    11701 cattcgcttt tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt
    11761 tagcttatgt ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt
    11821 gctcttgtta attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg
    11881 acccttattg aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga
    11941 ttcgatgatt acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca
    12001 tgtcagtatt gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga
    12061 attaacaaca ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg
    12121 ggatggtgaa agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa
    12181 aaagcagcta aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag
    12241 ttttgatttc tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct
    12301 ttctcctaaa aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt
    12361 tgcaggagct gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac
    12421 atggggtgga cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg
    12481 cgtgcaagag attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga
    12541 cgagcataat gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg
    12601 tactgaccaa ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg
    12661 tttagctgtt tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa
    12721 agaggttgtg acaatttttg tcacaacctt ttttgccgag taaacggtgt tcaaaagcca
    12781 gtttctcggt atgaagtcaa atttaagcaa aaatgtgaga agctattttc acaaaatttt
    12841 tcttcatact cgaaactaag cggctttttc acgacctctt ttttattttg ccatgaacat
    12901 atcataatac gcgtccatta tttctttagc aatcgtcaag tttatgtggt cttgttcgtc
    12961 taataagttc ggcaagacaa cactgatggc aatttccgga ttgtctgttg gtccgtatgc
    13021 cacgatatta ctattgactg tcgcaatatc tggatgacct tccgcaaccg tttctgcagt
    13081 ccctgttttt gcggaaaggt ccattttcgc actggctaat ggtttggctg ttgtgtaagg
    13141 atcggtacca tggacaactt ggtggaatcc ttcacggagg atcgccatat tttctgcaga
    13201 aatattgacc gtattttcta ctgttgtgcc aatttctttc ttcacttcac ccaagtttcc
    13261 attttcgtca ttgccataga tcgctttgac taggtgcggc tgtactcgtt tcccgccatt
    13321 agcgactgtt gaagcatatt gagctaattg gattggtgta tatgtgtcaa actgtccaaa
    13381 tgccaaatcg gtaaagttac ctggagcata ccattctttg ccattatccg cattgaactt
    13441 cttcatataa tcaacagaac gtgagatccc aggagattca ttcggcaaat cgatcccagt
    13501 cgttgtacca agaccaaact ctttgaaaga gtctctcaat tgctgataag ctttagcttc
    13561 ttcatctaaa ctaggtagtc ccattccagg tgtatagtct agtcctaaca tctttaacgc
    13621 cactttgatc atatacgtat tagaagacaa ttccgatgct ttgactgcat caagagcaac
    13681 ttgtccgctt cgattaaaga cagagctttt ttctgaagca ccttgcagac gaatcggctc
    13741 atcaatcaaa acttgatttc cgctgatcgc gttacttgcc cagcctgctg tcaaagtacc
    13801 agccttgact acagaccctg gatcaaaggc gctagtaatc gtgcctaaag cattctcggt
    13861 gatttctttg gaaccttgtt catgactgaa accagtcatt gctagcactt ctcctgtctg
    13921 aggattcata gccactgcat aagcaccagg cgaatactgt gctttgccgt tgttgatcaa
    13981 tgtttgataa ttccttttca aaatttcttc tacttttgat tggaattctg cattcataga
    14041 taacattaaa ttagaacctt tttccccaga gaagatttct ttttgattcg aaacatttcc
    14101 ttcattatca agggaaactt catattgggt tttggttcct tgcaggacat cttcatattg
    14161 tttttccaaa taactttgtc ctacacggtc attccgagaa taaccttttg ctaggtattt
    14221 ctctgcttca tctgctggaa gtccttgctt ttcagtcgtc acactgccaa gaatactttt
    14281 cagtgaactt gctgcattat attctcgtgt ccagtctgtg ccagtcgaaa ctcctggaag
    14341 ttcagaagcg cgttctgcca caacagctaa ctcttgatca gtaacaccag agtttttgat
    14401 atagacagtg tttaattcat atgcactatt catccgttta aagatggtag caatttttaa
    14461 ttgctcctcg tttaaattaa tctcatcagc tgtgacttta ttgaccatcg cactatattg
    14521 ttcgctgttc cctaattgct tttcttttgc cgaaagtctt tttgtggctt ctttcaaatg
    14581 tttgggatca gccagccaaa aatctttttt atctctatct gtcagatttt catctaccgg
    14641 aacatcgatc agcgagttca gcttcgttgc gatttcaagc aaatctttag ccgtaacttg
    14701 tgttcctctc gtatatgtga tggcctgatt cgctttattc gtaactaaag cttttcctgt
    14761 ggagtcgtag atcattccgc gaggagtact ttcttggacc gtaatcgtag aagtagactt
    14821 cactcgcgcc tccatctctt cgccgttcac gatctgcaaa taacccaatc tagcaatcaa
    14881 cgcaacaaat aacgtaaata taatgaaaaa aagaaaattt aagcgaaaag gaacatggga
    14941 ccggcgaaac gttttgttct taggttcttg tgcacctttt cttatttttt ctaaccaaga
    15001 agtatttaat atttttttca taaaatcttt catcatttcc tatccttccg cctatttaca
    15061 ttcatgcttc attgtaccca aaaacttctt aataa
//
LOCUS       DACEMS010000061        15009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEMS010000061 DACEMS010000000
VERSION     DACEMS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.062"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="IW735_001690"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="IW735_001690"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1044692.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1197..2216)
                     /locus_tag="IW735_001691"
     CDS             complement(1197..2216)
                     /locus_tag="IW735_001691"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1044693.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2849..3853)
                     /locus_tag="IW735_001692"
     CDS             complement(2849..3853)
                     /locus_tag="IW735_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1044694.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3866..4915)
                     /locus_tag="IW735_001693"
     CDS             complement(3866..4915)
                     /locus_tag="IW735_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1044695.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4932..5753)
                     /locus_tag="IW735_001694"
     CDS             complement(4932..5753)
                     /locus_tag="IW735_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1044696.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5740..6519)
                     /locus_tag="IW735_001695"
     CDS             complement(5740..6519)
                     /locus_tag="IW735_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1044697.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6536..7006)
                     /locus_tag="IW735_001696"
     CDS             complement(6536..7006)
                     /locus_tag="IW735_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1044698.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7018..7437)
                     /locus_tag="IW735_001697"
     CDS             complement(7018..7437)
                     /locus_tag="IW735_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1044699.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7518..10286)
                     /locus_tag="IW735_001698"
     CDS             complement(7518..10286)
                     /locus_tag="IW735_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1044700.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            10486..10710
                     /locus_tag="IW735_001699"
     CDS             10486..10710
                     /locus_tag="IW735_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044701.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11489..13204
                     /locus_tag="IW735_001700"
     CDS             11489..13204
                     /locus_tag="IW735_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044702.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13207..14970
                     /locus_tag="IW735_001701"
     CDS             13207..14970
                     /locus_tag="IW735_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044703.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4362 a         3096 c         2585 g         4966 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa
     1201 cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt
     1261 ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca
     1321 cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt
     1381 cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc
     1441 caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg
     1501 ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct
     1561 cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca
     1621 ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt
     1681 gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga
     1741 tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta
     1801 ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact
     1861 tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc
     1921 ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca
     1981 acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata tccgataatc
     2041 gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca
     2101 tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt
     2161 tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat
     2221 attcctccaa ataaataatt gataatttcc attatagtat acatttctta aagttgggac
     2281 taatttccat tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc
     2341 aatgatatgt tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct
     2401 aagaggaagc agttttgaca atgttactac cagcattact attaggaggt taggaaactt
     2461 ctcctagtga gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc
     2521 aggtcctgca acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt
     2581 caacattctg gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta
     2641 ctacggatcc atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca
     2701 catgtcccat taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata
     2761 acgtaattga tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat
     2821 tttttatatt ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta
     2881 tctctaatgg atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat
     2941 gcatcaataa gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat
     3001 agtcttttgt gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac
     3061 gttcagataa cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt
     3121 ctacgatctc taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc
     3181 agctttgttc ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg
     3241 ctccaataaa ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg
     3301 catctgcttt tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat
     3361 agtattctga ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa
     3421 aaaatttgat ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag
     3481 acaacttact gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt
     3541 gttctactgc tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag
     3601 aatcttttgt taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga
     3661 caggcaagtt gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc
     3721 caccgattcc tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt
     3781 cttctacttg cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat
     3841 tgaatttcag catgatttcc acctcttata atttactttt caagactgta tcaaagtctt
     3901 caaaaatctt tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt
     3961 gaaacggaac gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa
     4021 gtgttttctc gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac
     4081 tcatatatct ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga
     4141 atagcaaatg cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa
     4201 atccttgtga cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc
     4261 cccagtttgg accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat
     4321 atccatcaaa aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc
     4381 gcaactgttg catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa
     4441 ttcctatccc taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc
     4501 ctactgattc taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac
     4561 tttgcacgtc atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg
     4621 cttcaacagt agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag
     4681 gagaaagttt tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac
     4741 cataagattc caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta
     4801 agatcagtaa attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca
     4861 agatcgtact tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct
     4921 ctgctccttt tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg
     4981 atcaagatcg ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct
     5041 ccaagacata agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt
     5101 tctcctgaac cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca
     5161 acgaccatca gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc
     5221 tggatctttt tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga
     5281 aacagaatgt gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct
     5341 tgcatggcta aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa
     5401 gaatctccta accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc
     5461 acatcaaatt caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc
     5521 agtgtaacga tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc
     5581 atttcttctt tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc
     5641 atattcatct gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg
     5701 tgatttagct cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc
     5761 tacctcccct gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac
     5821 aactgctaaa atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc
     5881 tatagcaaaa cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag
     5941 catcgcaaaa ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa
     6001 gtcaggaatc atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat
     6061 taccacaaca ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac
     6121 tcctttataa tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa
     6181 aagccctaag taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa
     6241 aagcgcggtt tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc
     6301 ggtcacaaca tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc
     6361 taaagttgcg cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag
     6421 ccccgtcaca atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac
     6481 aaatgcaaca attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca
     6541 aaacttctct tacatccact tttttatctg ctggtacttg acggatttct acttctattt
     6601 gtttgtcaat taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc
     6661 cgatgctgcg agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg
     6721 gatacgcttc tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat
     6781 atttatctgt caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta
     6841 gtttgactcc ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta
     6901 catcatcatt agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta
     6961 cttgtccgtg taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta
     7021 aaattcgtct tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt
     7081 tgaatcagtt agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga
     7141 tactagctca atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt
     7201 gatatattgt aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat
     7261 ttctttatct ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc
     7321 atcagtatag caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga
     7381 atctaatatc cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt
     7441 ttcatctcct cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg
     7501 caacaacttg cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc
     7561 aaaatatcat agatataggc aatctcagaa ttggggatca tgacactata atgctcttca
     7621 atgacactaa acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc
     7681 tctaatcctt gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc
     7741 acatggacat acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct
     7801 aattctctca taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg
     7861 gaatccagta ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc
     7921 tctgaagcat tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag
     7981 atgaggtctt ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc
     8041 attcctacga cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc
     8101 tgatattcat atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt
     8161 tcacttaagt ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt
     8221 ttgttttcct ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct
     8281 gctatctctt ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt
     8341 gtcgtcacat tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg
     8401 atttctttca gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt
     8461 tcgcaataat caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca
     8521 aatgattcaa atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca
     8581 gttgcataac cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc
     8641 gcttttttta gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat
     8701 ttgatcttac ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga
     8761 gaaacttgtt gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat
     8821 ttgatagtgc ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat
     8881 ttgatttgat aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa
     8941 accagcagct cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga
     9001 atcacttctt gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa
     9061 tcaattagga tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc
     9121 attaattgct ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta
     9181 gtaataggct gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc
     9241 tgttcacgct gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct
     9301 cctatgtttc cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac
     9361 aaagggctcc ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt
     9421 tcattacgac tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg
     9481 aaagttgtta agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg
     9541 atccggcgtg acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg
     9601 aacagttttt cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca
     9661 ccatctgctg cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta
     9721 acatgtccaa acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta
     9781 aaagtgatga atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc
     9841 aaattaacta aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg
     9901 ccatctggat agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg
     9961 tatccgatca taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg
    10021 agatcatcga tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct
    10081 tctttgtgga aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt
    10141 aactggttca aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt
    10201 tgtgctgtaa agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag
    10261 taacataaaa tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat
    10321 tcattttgtc aataaaatct ttaataaaga attgaaaaat atatcttatt atagacagca
    10381 gcctacttat cccttataat ttttattcta aaaaacgtac agttccttcc agtttttata
    10441 ctggaatttc aacatatgaa agaataagga ggctatgact attcaatgaa atcggataaa
    10501 tataaaaaca ggaaacatac ttcctggaaa acttttcgag aaatcatcgg atcttatctc
    10561 gctgtagttg tatttttgcc tttgctttat atggctatga cagaacaaac tcgttttatt
    10621 ggcgtagata cgcaatatat agccgaaaca ggatttttat tcatctttat cttgattttt
    10681 gcgttctata aatccttcca tacaaaataa gattcacaga tttcaacttt cctgtctgtt
    10741 tttagctatt acaaatcctt atatgtggta taacaacaat ttattggcat aatgaggaaa
    10801 aacgagttat gatgctttca gagagtaaag aggtactgtt gtcaccatcc tttttattga
    10861 ttacgtacac cactcttagt tttttcccat tgattacgaa aacaaggtag aaacattgat
    10921 gttcctacct tgttttcttt ttttattttc agatcaaaat ctcaagtagc ttttgacttg
    10981 atgtcgggag gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa accatagttt
    11041 tctccactat tatttattcc tctaagatag tacccacaat aaacgaataa atatttttct
    11101 cattttcata ctccttcttt gaaaatcaat tagatttctc tcaacccact tttttacaaa
    11161 aattacgtag caaaaaagat tgcaagaaca aattaaaaaa gaagtaaata gagtttcatc
    11221 tataaacatt tacttgatat attttttatg cagaattcat ttatagaaaa attaagtaaa
    11281 atagacgttc ctatgaatga agagactggc atgttttctc attttctttt ttttaaaaaa
    11341 aatagtctat tcaatttcta aagcttcttc ataataaaac tttcatttat gaaaaggctt
    11401 gttctctttt cattctaaat ttcctaagct acaatgtggt agtacttgtt ttttcaattg
    11461 tggacaaata aataatagga ggaatcatat gattcagtta ttacgttatg caaaagacta
    11521 tcgtaagcaa atcattttgg gcccgttctt caaatttctt gaagctgtct tcgaacttgt
    11581 cctgccttta atgatggctt ccttgatcga taatggacta aaaatgaatg accgaggaaa
    11641 aatcattgaa atgggcttat ggatggtcgc tatgtctgtg attggtctga tttgtgctat
    11701 catttgccaa tactatgctt cagtagcttc acaaggattt ggtacagagt tacgtaatca
    11761 gctaatcaaa aaaatcaata ctttctctca caacgagttg aatcattttg gaacagatac
    11821 cttgatcacc cgaatgacaa acgacatcaa tcagcttcag ctagctttag caatggtcat
    11881 tcgtttattg atcagagcac cgtttttaag tatcgggtca gtggtcatgg cttttgtgat
    11941 cgactgggaa gtaggtctgt ttttcttagc attattgcct attttctcaa tcattttatt
    12001 ttttatcatc aaaaaaaccg ttcctctcta ccaaaaagtg caagaaaaac tagatcagct
    12061 caatgaacac gttagtcaga atcttagcgg tgttcgtgtc atccgcgcat ttgcgcagac
    12121 aaaaaaagaa acagagaagt tcgataaaag cacaaatgat ctagcgaaca attatttacg
    12181 tgtttccaat atctctgccc tattatcacc tgctacaacc ttgatcatga atgtcgggat
    12241 catctgtttg ctgacagttg gcgggatcaa agtaaatatt ggtagcttgc aacaaggaca
    12301 agttcttgct ttgatcaatt atatgaatca aatgcttttg gcactgatcg ttgtttcaaa
    12361 cctcgtcgtg atcttcacac gtgccgaagc ttcaggaaat cgggtgaaag aggtgctaga
    12421 cacagaaaat tcgattcttg aagctgaggt cagtactact cccgattttt cttctgaagc
    12481 aattatcgct tttgaccatg ttgacttccg ttatactcct gattctggtc tttctctcca
    12541 aaatattaca tttcagctga agcgaaatac agtccttgga atcattggtc caaccggaag
    12601 cgggaaaacg actttgacac aattgattcc gcgtttctat gatgtcagtt ccggtacagt
    12661 tataatggac ggaatggatg ttcgttcttg gcagctggat catctccgtc agcaaattgc
    12721 tcttgttccc caaacttctg tactatttac cgggactatt cgtgaaaacc ttcaatgggg
    12781 taagaaagac gccacagaaa aagaatgttg ggaagctctg aaaatcgctc aggcagaaga
    12841 atttgtccgt cagcttccaa atggtttaga tacaaaagtc atggaaaatg ggaaaaattt
    12901 ctctggcgga caaaaacagc gactgacgat tgcaagagct ttgattgcca agccagcatt
    12961 actgatttta gatgactctt taagtgcgtt ggattatcaa acagacttgg atttgcgtaa
    13021 ggcgctacag acttatcttg gcagtacagt gattattatc tcccaacgtg tccgctctat
    13081 tcaagaagca catcatatct tagtgatgga tcaaggtaag attgctgctc agggtaccca
    13141 tgaagaatta cttgctcaat cagcagaata tcaggaaatc gtcgcatcac aggaggaaga
    13201 atagttatga aagtaaaaaa attatccttg cagacgctcc gccgattcat tccttacctt
    13261 actgcctatg caaaagacat ttggctagct gtgatactag gaatcatcaa tgggattgca
    13321 acagttgtga tgacgttaca aattggtcaa agtatcgatc aaatgattgg agaaggacag
    13381 gtccgcttca atcgactgtt ccatttactt attctattcg gtggtatcgt tttaatcaac
    13441 gtagtcagtc aatggctcat ccaagtgtta agcaatcggt tagcttactt atccgttgca
    13501 aaattaagaa aagatgcatt tgctcatctg aatcgattgc cacttcgtta ttatgatcaa
    13561 aactcccacg gaaatatcgt cagtcgattt acaaatgata tggacaatat ttccattgca
    13621 atttctgcgg ccttcaatca attattttct ggagtggcaa tcatcattct ttcgtttatt
    13681 tttatgatgc gtctaagtcc acttctgaca ttggtcgttc tttgttctac accaattatt
    13741 ttccttgtga gttggatcgt tgctcgtgct tcacaaacaa atttcgatag acagcaaagg
    13801 attattgggg atatctcggg ctttgtgacc gaacgaatcg gtaaccagaa aatcgtgaaa
    13861 gcttttcagc aagaagaagc tagtcaacag caatttgaac tgctaaatgc tgatctatat
    13921 gaaaaaggac aacgggcaca attttcttct tccttgacaa acccgtcttc tcgttttgta
    13981 gatcaccttg cttatttatc gattggttta gttggcggcc tattggcatt gaagagtggt
    14041 tcgtcaatca ctgttggtgt gatttccagc tttacgatct actcaagtca gttttctaaa
    14101 ccatttatcg aactttctgg aatcacgaca caactgcaaa ccgctttttc tggtctagaa
    14161 cgagcatttg atttgatcga tcagccagaa gaaaagcctg atttgccaga tgcctatgcc
    14221 ttggaaccca aaaatatcaa aggaaaagtc tcttttgatc atgtttcttt ttcttatgac
    14281 cccaagcagc gtttgatcga agatttcaat tttactgctt atccaggtca aaccattgct
    14341 atagtaggaa aaacaggggc cggaaaatcc acacttgtta atttgctgat gcgtttttat
    14401 gacacaacaa aaggaacaat tacagtcgat gaccatgaca tccgctctat ccaacgtgat
    14461 acattacgta aaagttttgg aatggtgtta caagatactt ggctatttga cagtacattg
    14521 cgagaaaatc ttcgttttgg gaatccggaa gcaagtgacg cagaaatcaa tgatgcccta
    14581 aaagcctctt atatgtatga atttgtagaa cgtttgccgg aaaaattaga tactaagatt
    14641 ggcggtcaag ggttaaagat atcagatgga caacgccaac tgttgacgat tgctcgaacg
    14701 atgatcagta atccccctat gttgatctta gatgaagcta ccagctcagt cgatacattg
    14761 acagagaaaa aaatccaaca agccttctta gcaatgatgg aaggaaaaac cagttttgtg
    14821 attgcccatc gcctgtccac aatcaaaaat gcggaccaga tattagtgat ggatcaggga
    14881 gctattgtcg aaatgggcac ccatgatgag ctgcttcaaa aagatggtta ttaccgacgg
    14941 ctctatcaag cacaattcac agcacaataa aaacaagcag tcttctggga tgggaagact
    15001 gcttgtttt
//
LOCUS       DACEMS010000062        14968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEMS010000062 DACEMS010000000
VERSION     DACEMS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.063"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(118..432)
                     /locus_tag="IW735_001702"
     CDS             complement(118..432)
                     /locus_tag="IW735_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1044704.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(446..706)
                     /locus_tag="IW735_001703"
     CDS             complement(446..706)
                     /locus_tag="IW735_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1044705.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            876..1190
                     /locus_tag="IW735_001704"
     CDS             876..1190
                     /locus_tag="IW735_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1044706.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1192..2847
                     /locus_tag="IW735_001705"
     CDS             1192..2847
                     /locus_tag="IW735_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1044707.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2930..5065
                     /locus_tag="IW735_001706"
     CDS             2930..5065
                     /locus_tag="IW735_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1044708.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIYNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5240..5749
                     /locus_tag="IW735_001707"
     CDS             5240..5749
                     /locus_tag="IW735_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044709.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            5746..7050
                     /locus_tag="IW735_001708"
     CDS             5746..7050
                     /locus_tag="IW735_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1044710.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            7146..8015
                     /locus_tag="IW735_001709"
     CDS             7146..8015
                     /locus_tag="IW735_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1044711.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8127..8447)
                     /locus_tag="IW735_001710"
     CDS             complement(8127..8447)
                     /locus_tag="IW735_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1044712.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            8601..8996
                     /gene="dhaM"
                     /locus_tag="IW735_001711"
     CDS             8601..8996
                     /gene="dhaM"
                     /locus_tag="IW735_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1044713.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            8998..9981
                     /gene="dhaK"
                     /locus_tag="IW735_001712"
     CDS             8998..9981
                     /gene="dhaK"
                     /locus_tag="IW735_001712"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1044714.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            10009..10614
                     /gene="dhaL"
                     /locus_tag="IW735_001713"
     CDS             10009..10614
                     /gene="dhaL"
                     /locus_tag="IW735_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1044715.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            10761..11426
                     /locus_tag="IW735_001714"
     CDS             10761..11426
                     /locus_tag="IW735_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044716.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            11709..12368
                     /locus_tag="IW735_001715"
     CDS             11709..12368
                     /locus_tag="IW735_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1044717.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12445..14055)
                     /locus_tag="IW735_001716"
                     /pseudo
     CDS             complement(12445..14055)
                     /locus_tag="IW735_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(14068..14949)
                     /locus_tag="IW735_001717"
     CDS             complement(14068..14949)
                     /locus_tag="IW735_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044718.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
BASE COUNT         5030 a         2737 c         3110 g         4091 t
ORIGIN      
        1 acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga agtagcttct
       61 tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct ctactgacta
      121 aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga cacctgctgg
      181 tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac gcatagcatt
      241 ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg cagagtctag
      301 ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg gagaagcttt
      361 tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat caatatggaa
      421 aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc gcttgttcta
      481 gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt ttcagattat
      541 ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt aattgagtga
      601 tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga atttgtccct
      661 ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca cattctcctc
      721 tatttttata attagataat accccttatc ggtattttat gcaaatataa aaagtagtga
      781 aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa tataccccat
      841 gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt caatcaataa
      901 aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg ttacttgatg
      961 ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat gttccattac
     1021 aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt tgtcaatcag
     1081 ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat gtagtgaatg
     1141 tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa gatgaaaatc
     1201 gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg ccgattaatg
     1261 gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc aaattgcggc
     1321 ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt ccaaacgcca
     1381 gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt gacagccatc
     1441 tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat tgaacagtac
     1501 gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc agggataaca
     1561 gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt gatgcatgca
     1621 ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg attggaaatg
     1681 gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc accacatatt
     1741 cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc tcatcaagga
     1801 gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa acgattgcga
     1861 ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg tgttcgtcct
     1921 gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg tatcctagta
     1981 gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc tatcgttgta
     2041 aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc caatcgtcaa
     2101 ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg cggtattgga
     2161 acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag tgaaagaaca
     2221 gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga tcatgcaagc
     2281 tattatccag aagcaacaga tattttactg aagttgatct tccatccaga gactggcgag
     2341 atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga tattttagca
     2401 acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt gacgtatgca
     2461 ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc aatgaacatt
     2521 gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact agcaaaagga
     2581 aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt caaggaagcc
     2641 aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag ccaacaagaa
     2701 tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat cttgaagcag
     2761 tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac tgtccgacct
     2821 gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc gactcttaaa
     2881 agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta tggtcaacac
     2941 acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa ttgataaaaa
     3001 aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt cgttgaaaga
     3061 aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg atactttttc
     3121 gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga aaaacgcaga
     3181 aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa aagtcgttgt
     3241 agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa atttcacaga
     3301 tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac ttttttacaa
     3361 taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata cggttattaa
     3421 agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg atacgtctgt
     3481 agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca atgaaatgaa
     3541 ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg tgaaaagtca
     3601 aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca tgcagcgtat
     3661 tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc tgattctatc
     3721 acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag gtgaaagaaa
     3781 tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg aaatagaagg
     3841 tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga cagaacgttt
     3901 gcgtagcaaa aagtttatct ataatctaaa acaatcagat cctttgtttt tagatcatcc
     3961 gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa aaacgatcga
     4021 aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc aagcaacgaa
     4081 aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa gtcctgctgt
     4141 gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg gtaaattgat
     4201 ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac aaaaaaaata
     4261 tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc agttgtttga
     4321 aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac gtactgttga
     4381 tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa atgtaccagc
     4441 atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat taggtctatt
     4501 gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc aggcaatctt
     4561 taattcttcc actacttttg ctgatattat gcgttgtttc gacgactacc atctgccgat
     4621 ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag agcttttcaa
     4681 aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc aaggaaaaga
     4741 acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag ctgagatttt
     4801 ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg aaacccaaac
     4861 gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa tcattccttt
     4921 gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg aaaacgatgg
     4981 agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa tgaaaagcat
     5041 tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac aactgataaa
     5101 aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc ttaggaaaaa
     5161 tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac gtcgtaacaa
     5221 aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat caattttggt
     5281 gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa cgttagtggc
     5341 tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc cattaacgag
     5401 accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga aaatgctgta
     5461 tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct catttgtatt
     5521 gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg aatatgaaga
     5581 tgaaaaattg aaaaaaagac aagaattact tgaagaagtg tatacggaac gttttggaac
     5641 aaaagaagag agagaaacgg tccgttttta ttctgtctcc gaagagaaaa atctagatac
     5701 gacattcatt gcagatttgt ataaagaaaa tggggtggaa ctgaaatgat ctcaatcacc
     5761 catacaggta atttattctt agatacttgt ttgtctatta tgtatttttt tctcgtttct
     5821 tatccgattt taggcggatt tgtatggttt attggtgtct ggtgttatgt gtttatatac
     5881 aaacataagc aaaaagaatg ggtagatgtt cccttgagtg tcgaaccgtt catcacgatc
     5941 atggtgccag cccataatga agaaattgtg atcgaatata caatcgagta tttgatgacg
     6001 aaattgaatt atcacaacta tgaggtatta gtgacagacg atggttcaac ggaccaaaca
     6061 ccagaaatat tagctagact gatgaaaaaa tacgctaatc ttcgtgtggt tcggatcgaa
     6121 aaaaataaag gaaaagccca cgcgtttaat atcggacttg cctttgcaaa agggaaattg
     6181 attttaagca atgatgcaga cacggtaccg gagccagatg cactgattag gtatgtgaat
     6241 tatttcattc gccctggtgc aagacatatt gctgcggtca ctgcaaatat ggatgtccaa
     6301 aatcgtacca aattgattgc aaaatctcaa acggttgaat tctcaagtat cgtgggcatc
     6361 attaaacgaa cacagtcagc tgttttcggt ggtctttatg cgtacagtgg agcaaatacg
     6421 atgtatcgaa aagaagcctt gatcgatgtg ggcggattca gacaagatcg agcgacagaa
     6481 gaaatcagta ttgcttggga tcatcagtta aatgactggt tatctgtttt tgctcctgag
     6541 attatctttt tcatggaagt tcctgtgacg ttgaagatgc tatatcgaca cagaaaacgt
     6601 tgggcaaaag gtggaacaga agtctggctg acgaatttca aaaaagtgat gctccatcct
     6661 tttaaacata ttggaagaac gatcatcttc attgatcaaa cattgagtat tgtctggtca
     6721 atattctttt gtatttccgt agttcttttt gctggattaa tcgggcatta tgtatatcaa
     6781 ggaaactacg aacagattta tattactttt acattcagtt ttgtgttcat ctgctttgaa
     6841 atggtcgcag gatttttcca gttgctagct tcgctgattg tagatgatcg acgaagaaaa
     6901 ttgaaatacc ttcttttcgc accactatat atgcttttgt tctggatagt gaacgcgata
     6961 acgatcgtga cgactttcat tccagcagtc aaaacgattt taggttatgg aagcgggaca
     7021 tggaaaagcc cggaacgtac aaaaaaataa aagatgcacc gttttttaga caaacagcct
     7081 gttgtgttta tggttcttcc aaacattttt gcttataata agaaattagt taaaaagtag
     7141 taaggatggg aaaagtgatg aaaaaaagaa tgaagccgtt aatctggcca attgctgttt
     7201 ccagtatttt cttattggga cagacagctt ttgcggaaac ttctgaacct gctcaaacag
     7261 aaccttcaac aaccataagc tcttctggtg ctgaaaacgg tgcctcggaa acagaaatga
     7321 cttccaatgt accgacagat aattcaggag aaacgatttc atcttcagaa gatacatcaa
     7381 taaatagttc tacctcttct gaccaaatcg atcatttgca agacttggaa aataaaagaa
     7441 aagaactcga agaagcatta gaagctgcaa aaaacaaatt gaaagaagac caagcagcaa
     7501 tcgatcaaga tttagctgat cttaaaaata aagtgggaaa tgaccgtgca caacttgaga
     7561 aaaagcttgc cgaagcgaaa gcaaaagcgg aagctgtcca aacagatctt gacgggaaga
     7621 tccaacaagc aaaagaattc aatcagcaat taaatcaggt tgctcaaaca gcttctggaa
     7681 agctaagtca aactcaagcg gatatcacag cggccagtca agcaatccac ggaaagatca
     7741 gcgaagcaca aacagttgct aatgattttg aagcaaaaaa agctgactta gcagctagtt
     7801 tccaacgaat aaaagatatt ttgcattcgg caggagaaaa atcagtgacc tctggaagca
     7861 cgacagaaac agttcagcaa acgacagagc catccaaatc aacaagtcag actttgccaa
     7921 aaacaaatga aaaaacagct tctgtcagtt attccttagc aggggcgggt gtgttagtcg
     7981 cagcagctta cggaatcatc cgcaaaaaga aataaaaaag agaggatggg aaggtagagt
     8041 acctcgaagt tagactgaaa aatctaattc ttgagtgcca ctacatgtcc catctctctt
     8101 tagttattaa aacagatgtc ccctacttat ctcgtggaga cagctgttcc actacatgtg
     8161 accatcagca tgccattgtc gctgcctaat acttcatagt ccatttttac gccaatgatc
     8221 gcatcagctc ctaatgcttt tgcacgttcg gccatttcgg caagtgcttc ttctcttgca
     8281 cccattagtt cgttttcata gctttttgag cggccgccaa agatatttcg caaccccgcc
     8341 tccaagtctt taaatgcatg gatcccagta atgacctcac caaaaacgat ccctttataa
     8401 gctactacgg cttttccctc tacactgttc gttgttgttg taatcattcg atcactcctc
     8461 tcttttaatt aaagtataac aaacttcaag aagcactaca catattttcc caaaattaga
     8521 aatttattat atacttgaat tgttcattaa tgaaagcgaa accaatcgtt agcaactatg
     8581 taaaagaaag gaagattctt gtgaaaaaaa gtattttgct tatttctcat agtcaaaaag
     8641 taacagatgg tattaaagaa atgattgagc agatgcaaaa atccgaagag gtgcaaattt
     8701 tttctctcgg cggaataaat ggcgagatcg gatcagaccc gatgaaaatc gtagaagcag
     8761 tcaatgaatc aagcgatgca gctagttatt ttgtttttgc ggatatcggt agcgcggtat
     8821 tgaactcgga actggcaaaa gacatgttgg aagaagagca gcagcaacgt tatcatctgg
     8881 tggatgcgcc tttagtggaa ggagcttttg cagcagccat cactgccggt ataacagatg
     8941 atactcagca aatcttaaca gaagcacaaa atgccggaaa gaaaggatgg gaataaattg
     9001 aaaaaaatca tgaatgatcc aagcaatatt gtcgaagaaa tgctggaagg attagtaaaa
     9061 agctatccag aacttgttca tcgtgtagaa tcttcccgtg ttgtagccaa gaatcagaaa
     9121 gcggaacaag ttggtttggt ttctggtgga ggaagcggac atgaaccatc ccatgccggg
     9181 tttgtagggg aagggatgtt aagtgcagcg gtgcttggtg atgtgtttac ttctccaact
     9241 ccagatcaga ttcagacggc tattaaggaa gccgatagtg gaaaaggagt tttgctgatc
     9301 gttaagaatt atacagggga tgcattgaac ttcgatatgg caaaggaatt agctgctatg
     9361 gacgatattg aagtagaaag tgtgatcgta gatgatgata tcgccgttga aaatagtacg
     9421 tatactgctg gaaaacgcgg agttgcaggt actgtccttg tacacaaaat cgtgggcgat
     9481 gcagcaagaa acggtgcttc cctagctgag ttgaaagaac tgggagaaaa ggtcgtacag
     9541 gcaactaaga cgatcggtct agcgcttcga gcagcaactg taccagaagt agggaaacca
     9601 ggattcgaac taggagaaga tgagatcgag tatggtgttg gtatccacgg tgagccaggc
     9661 tatcgtcgag aaaagatgca gccttctaaa gtactggcaa aggaacttgt tactaagatt
     9721 ttagatgatt actcgaattt accaaaagaa gcaggtgtac tagtcaacgg aatgggcggg
     9781 acgcctttga tggaacagtt cgtgttcatg aatgatgttt tagctctgtt ggaagaaaga
     9841 ggcgtcaatg tagttttccg aaaagtcggt aattttatga cctcactaga tatgcaaggt
     9901 ctttctctaa cactgatcga cttgacggag acacaatgga aagattcttt agaaagcaat
     9961 gtacagacga tttcatggta aaaaatctca agataaaagg gtgataatat gaaactaacc
    10021 gttcaagaaa tccaagaatg gctcacgcaa tttgcagagg caatcaatca gaacaaacaa
    10081 tatctcagtg atctagatac cccgattggc gatggagacc atggaaataa catgggaaga
    10141 ggggtttcag cttatgaaga agcttttcaa gccgatcatc ctgaaacaat cagcgataca
    10201 ttcaaagtat tctctatggc gatgatatcg aaagtcggtg gggcttctgg tccgttatac
    10261 ggatctgcat tcatgaacat gatgaaagcg acaaaagagg tcgattctat tgattctcaa
    10321 gaaaaattag gcgaagtgat cgagcaggga acagcgggta ttcaaagccg aggaaaggca
    10381 gaagctgacg ataaaacgat gttagatgtt tggctgccag tcacagaggc attgaaggca
    10441 gggaatttaa caaaagaaat cattgaccag gcaaaagaac acaccaaagt cttggtagct
    10501 aaaaaaggac gagcttccta tctaggagag cgtgcaatcg gacatatcga cccaggagct
    10561 gcttctagtg ccattttatt ttcaacgctt ttagatgtga tcaatcaacc ttaagttcaa
    10621 taaaactgcg gtgatgctca tcgcagtttt ttgtttggct gttcttatga cttgatgcag
    10681 aacagctttt tcacaacctc tatttgtcca tatcgcaaaa ttcaggtaga atacaaaaga
    10741 taacggaaag aggcgaaaca atggaacttg gaaaatcaga ttatcagttg tttgatcgac
    10801 caatctacgc attcaaacag ctcaaagaga gccatccaac agataagatt gaacaaatca
    10861 agaaagaata taaagaacac tggcaaaaat ggaaagagat tcagctccag acagctgcac
    10921 ttttgcctga tatgtacggc atgagcaaac ctaaaatcga aagctggacg aatggctgga
    10981 atttacgcag tcatttttgg agcgcgtacc gaagcgaatc tcgtcaagac gagaatgcct
    11041 gtctagcagt tttgttgaat caaaaacaat accaaatcta tttgatgtac caacattata
    11101 aaagcgaaga acgatatggt agcattgcag cgtataacca gttgcttgcg atactgaaag
    11161 agtggagcaa aacagtcgat atcaaggatt attacatatg gccgcaacca gaacatgaac
    11221 ttgacgacca cttagctttg tctgactatt tgtccgatac aaagaaacaa gaagagttga
    11281 aaaaagcgat gagagaccga acctttcaaa tgggtaaact attcttctat ccgcaagaaa
    11341 tcgaacacgt tgagcaaata accgctgaaa ccttacaaga actagcacca ctatatcaaa
    11401 agctaggtgc ggaaaaattt caatagacaa cacgaaataa gcaatgcgaa aaacaaacct
    11461 tactctcggc ttgcgagtga aggtttgttt ttgattattc aaaaagtaaa tatttaaaaa
    11521 attgatagga atcaattact taaggtctca aaagaaggaa aataaagcaa gggacaaaaa
    11581 gaaatccact ctatttgctg gtatctggtt cgcttgacgt ttatgctatg ataaaagaaa
    11641 attatcagtt ggaataaggg aagttatgtt tctttattag aaatagctgt ttagaaggag
    11701 gagagattat gagtcatgat catttatgtg tttcatttgt tcctttgttc aatcacttag
    11761 tacagcaaga tcaagaaaaa gtacacgcac ttgtcaaaca tcactatgtg gaaaaaggag
    11821 aacagattct tgcgccgaat ggagaacctc aattagtgat cgtggcctcc ggcagtatga
    11881 aagtgtatca attatctgct agcggaaagg aacagctttt acgagtaata gagccgggcg
    11941 gatatgaggg agagagccag ttgctaggcg ttccaaatga gtctttattt ggcgaagcac
    12001 tagaaaaaac ggaaatttgc atattgagac aaaaagattt ctcggaactc ttactgcatt
    12061 atcccgccct tagtctgaaa cttttagcaa tcaacgttga gaaattaaca aaagtacagc
    12121 agcagacaac ttttttgatg atggaaaaag tagaagaacg agtcgctgtc tatctgcttg
    12181 acttagctaa agcgacaggg aaggatcact ttttcctgcc aatgaaaatg aaagaacttg
    12241 ctgcatttat cggtacgacc cccgaaactt tatctagaaa atttaaatta ttagaggaga
    12301 atggttatgt aaaaagaaag aatcgagaag tctggatcat tgataaagag cagttagaag
    12361 acttgtaaat aaaaaaatga cctccaaatg ttggattttt gacccagatt tggaggctag
    12421 catgaaattg tacatgcttt tttattatcc ttctagtaag tctcttcgcc tataaccaat
    12481 agaaccaagg acgaacagtg caaggctgat acatgataac gtaaggaaca cggaaagatc
    12541 aaaagaatcg tttggcatcc gtggaaccca gctagggaca gctgttttgg aaaaccattc
    12601 tggtaaatcc aaaattccgc caaagtaatt gagtgcaaag gagtaagcta agtagatata
    12661 agcaagttta ccgattcgag gaagccatcc gaatagaaag ccactaattc cgataaacac
    12721 taaaacggca ggcaggaaat tgaacccggc tttaagaaac atcaatagat ccatgtcttt
    12781 cgcatccttc atcgaagcca aagcagaaag ccctagtgca ccagaactaa tcaggattgc
    12841 caatatagca aatagaatag ctagtaacat attggtccaa taaagttggt tcctagaaat
    12901 ttttgttgcg aacagctgat tcatacgtcc gtgggtttct tctgtaaata aacgattgat
    12961 gctagcaatt ggtaaaatag cagctagaca aatcaagacg accatgatcg tgcttgtaaa
    13021 gtttttttca attgagacgc cagattggat aaacatctgt ttcattaagt cgttactttc
    13081 taaaaactgt tgcatattcc cgtagataga gccgtatgcc gcccccatca aaccaaaagt
    13141 gataagccaa ctgattgtca tccctttatt tagatagaat aataaaccac gcaaggataa
    13201 aagagaacgt ttagcatacg gttttccttc acgttcgggg atatagccgg tgttcaaatc
    13261 tcgatgttcc tctaaaacaa atgaactgat tatcaagatt agactgaaca aaaacaaata
    13321 aagcaaagga attcagttgt tagttgtaaa tggataggtc aaataagacc agcctaaagg
    13381 tttccacatc gaaagggagg aatcagctac atctgttatt gctcgtagaa ggtaaagtcc
    13441 acctaaaaac gctaaagaca gaccattcga actggctgaa cttggcatca gttgtgcagc
    13501 aaacagtgca acggcactcc ccaaaatccc catactacct acagatagcc caaaaagcca
    13561 agcaccttct gcagtgatcg aatccgcttg gaaaaaaatc atcgtacaac tgatcgatat
    13621 tgctaaaagc aaattgatca gaatactttc agcgatgaca gctaaagagt ttgcttgacg
    13681 tccgactcga aaggaacgaa taagttcaat cagaccaagt tcttcttttt ttctcgtttg
    13741 gctcaccacg tgtaaaacag agatcacagc agcaaacaaa ctgcaaaaaa gcaacatttc
    13801 atttgcatac atcgctccta aagtatactc agcagcattc gaagctggag ttggtccgac
    13861 cattgcaatc attgccgggt tttgcaaggt ttcgtataat ccagctaatc cttgtccgga
    13921 agtcaattct tcaaacgcag ggacaaaacc accagaaaaa gcagtgacac cgactagcca
    13981 tatcaggatc tttttccagt cacggctaag gttttgagaa aaaagggtgg accagcgtga
    14041 aaatttatca gtcatttcgc ttcctcctca tccttcataa tgtctcatga ataaatcctc
    14101 aagagtcggt ggaatagatt caaatttttt cacacctaat ttggacgctt cagctaaaat
    14161 cacatcgaaa gcttcgttat cagcagcaaa ttgagcgagg tgaccttttt gcacaaagtc
    14221 atggacaccg ttcaattgtg ctaagtgact attctctgct tctgtttcta tagtgatggt
    14281 cgaacgagtc aaatgacgca attcatcgag tgttccttct tcatcgagtg ttccttcgcg
    14341 tatgatcacg actttatccg ctagtcgctc gacttcactg agaatatgag aagaaagtag
    14401 gatggatttg ccctgttctt tcaatttttc aacttcttct tggaaaatcg tttccatcaa
    14461 aggatctaaa ccagaagtag gctcatcaaa taaatagaga tccgaatcaa cagccaaagc
    14521 agcaatcaag cccacttttt ggcgatttcc ttttgaatag ttttttgctt ttttcttagg
    14581 atcaagagag aaacgttgaa tcagttcatc ttttttttga gcactccctt ttccatgaag
    14641 cttcatgaag agatcgatga tcgctcctcc agtcaaattt ccccacaaag agatatcgcc
    14701 tggaacataa gaaagacgtt catggataga taaactatct tcccatacat cttgcccaaa
    14761 aatcgttgcc gttccgccgt ctttttttat aatccctaaa agtgtccgaa tcgtcgtgga
    14821 ttttccagaa ccatttggtc cgataaatcc aacgacttca cctgaatgta cggtgaaaga
    14881 gacatctttt aaagcttcga aacgaccaaa gctctttttc aaatgtgtga ctttgacgat
    14941 ttctgccata aaaaccccac tccttttt
//
LOCUS       DACEMS010000063        14574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEMS010000063 DACEMS010000000
VERSION     DACEMS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.064"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IW735_001718"
     CDS             complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IW735_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1044719.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1993..3216)
                     /locus_tag="IW735_001719"
     CDS             complement(1993..3216)
                     /locus_tag="IW735_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1044720.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3247..4086)
                     /locus_tag="IW735_001720"
     CDS             complement(3247..4086)
                     /locus_tag="IW735_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1044721.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4103..4783)
                     /locus_tag="IW735_001721"
     CDS             complement(4103..4783)
                     /locus_tag="IW735_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044722.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4785..5858)
                     /locus_tag="IW735_001722"
     CDS             complement(4785..5858)
                     /locus_tag="IW735_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1044723.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6505..7176)
                     /locus_tag="IW735_001723"
     CDS             complement(6505..7176)
                     /locus_tag="IW735_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1044724.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IW735_001724"
     CDS             complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IW735_001724"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1044725.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10600..11037
                     /gene="ndk"
                     /locus_tag="IW735_001725"
     CDS             10600..11037
                     /gene="ndk"
                     /locus_tag="IW735_001725"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1044726.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(11059..11925)
                     /locus_tag="IW735_001726"
     CDS             complement(11059..11925)
                     /locus_tag="IW735_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1044727.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(12085..14178)
                     /locus_tag="IW735_001727"
     CDS             complement(12085..14178)
                     /locus_tag="IW735_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1044728.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4229 a         3111 c         2481 g         4753 t
ORIGIN      
        1 cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct
       61 ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta
      121 ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc
      181 taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca
      241 acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg
      301 caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca
      361 aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg
      421 atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag
      481 gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa
      541 atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa
      601 ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat
      661 actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac
      721 aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa
      781 aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt
      841 ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt
      901 tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg
      961 tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat
     1021 atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc
     1081 ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag
     1141 cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat
     1201 aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg
     1261 gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc
     1321 tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat
     1381 tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat
     1441 cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt
     1501 taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc
     1561 taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat
     1621 gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt
     1681 ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat
     1741 taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta
     1801 attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta
     1861 accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca
     1921 atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa
     1981 gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg
     2041 ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc
     2101 gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt
     2161 gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt
     2221 tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt
     2281 ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa
     2341 agaaggagta acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt
     2401 gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa
     2461 tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt
     2521 ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa
     2581 gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt
     2641 ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt
     2701 cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc
     2761 gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc
     2821 ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt
     2881 cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc
     2941 aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga
     3001 aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct
     3061 ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc
     3121 aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag
     3181 atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca
     3241 tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt
     3301 ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt
     3361 attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc
     3421 cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc
     3481 gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa
     3541 ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc
     3601 ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc
     3661 atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt
     3721 caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc
     3781 agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc
     3841 aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc
     3901 caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc
     3961 tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg
     4021 gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt
     4081 tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa
     4141 attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt
     4201 ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc
     4261 cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac
     4321 caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg
     4381 cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag
     4441 caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg
     4501 ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg
     4561 aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc
     4621 cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga
     4681 atgctgtcca aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa
     4741 tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg
     4801 atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat
     4861 ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc
     4921 aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt
     4981 acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa
     5041 cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc
     5101 gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct
     5161 tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata
     5221 accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt
     5281 gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc
     5341 ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct
     5401 cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct
     5461 ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc
     5521 gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt
     5581 agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga
     5641 gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc
     5701 aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa
     5761 acatcgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt
     5821 tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa
     5881 aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc
     5941 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca
     6001 agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc
     6061 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
     6121 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac
     6181 ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct
     6241 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt
     6301 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
     6361 gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac
     6421 gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg
     6481 aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac
     6541 agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata
     6601 atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc
     6661 tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg
     6721 ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa
     6781 tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt
     6841 cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat
     6901 ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc
     6961 ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat
     7021 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg
     7081 gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac
     7141 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
     7201 aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc
     7261 gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc
     7321 agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag
     7381 cctaattcag caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta
     7441 gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc
     7501 agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat
     7561 aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca
     7621 gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt
     7681 ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc
     7741 aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga
     7801 ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc
     7861 agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata
     7921 ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct
     7981 aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg
     8041 atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta
     8101 tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg
     8161 aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc
     8221 tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact
     8281 aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac
     8341 gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg
     8401 tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca
     8461 ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt
     8521 cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc
     8581 atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca
     8641 acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc
     8701 ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc
     8761 agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt
     8821 ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta
     8881 tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact
     8941 gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga
     9001 agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa
     9061 tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg
     9121 atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca
     9181 gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct
     9241 tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg
     9301 ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc
     9361 aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc
     9421 atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct
     9481 gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca
     9541 gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata
     9601 gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc
     9661 gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct
     9721 ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa
     9781 ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt
     9841 tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc
     9901 tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg
     9961 tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat
    10021 aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct
    10081 catctccttt actaagtaat tttaattcat gacttccaat catttttcga atagttattt
    10141 ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa
    10201 cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat
    10261 ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct
    10321 tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg
    10381 ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg
    10441 ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt
    10501 ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc
    10561 ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt
    10621 atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca
    10681 aagggactgg ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa
    10741 cattatcagc acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc
    10801 ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg
    10861 gttggagcaa caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg
    10921 ccggggactg aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc
    10981 gcccgctttt tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac
    11041 aaattctaaa ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa
    11101 ggacctccgt tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc
    11161 tttcgtcttt tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa
    11221 tgtttcatag atcactgtac ttctttcttt atgtgctaag ctccgtttca tatcttcatt
    11281 cacactacgt gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa
    11341 aaaagtaaag agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt
    11401 gattttcaaa taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag
    11461 aatagatagt tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg
    11521 gcattcataa cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg
    11581 aagattcaaa accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct
    11641 gatctcttta taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt
    11701 ctttaacttt attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat
    11761 gttcattcgc tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt
    11821 ctcaatcccg actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa
    11881 cttcttgttg actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt
    11941 taatttaaat ttttccacat tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa
    12001 aaataaaaaa atgttttttt cataaaaaat accaaccttc aagcattaga atgttgtcta
    12061 actactgaag gttggtttga ttgtttattg attatttttt tgctgttcaa cgtctgttct
    12121 tgttactgga tataagttgg ttgttgtttt attaccattt tttgaaaaga cacttgtcga
    12181 atgttcgcct ttttgctttt cgattgcttc attacgttcc gcttcatttt tatagtcata
    12241 ttttttcgga tcaatcgtct caaatccgtt tggcacataa aaccgcaaga gattttcctg
    12301 attcagctta tctgaaagac ttaacgcagt cttgactttt tcctgatctt tggagatttc
    12361 ctcatcgatt cctgctgctt tcgtatcgat gatttctcct gtctgattat tgtaagcttt
    12421 tcctcctagg acggtgtatt tgggtgtcac aaagttccca tttcgaaaag caactacttg
    12481 atcatgctga gaagataata agtctgtacc aaaatgaaga taatctttat catcgattcc
    12541 caatagatgt agtaatgtcg gcaaaacatc gatttctcca ccgtattcat gattgactgt
    12601 ccctttagta taaccaggca tatggatcat caatggcact cgttgcatct gtgcgttgtc
    12661 aaattcatcc catgtatctg gatctttacc aagagcgctt gctaaatctt tattatctgt
    12721 attcgagatc ccaaaatgat ccccgtaaat cacaaagatt gaattttggt aaataccaga
    12781 ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc agataatgtg ccgttcgaac
    12841 ataattatct actgtgctat tgccagtatt caaagatgga aagtcgaaat tcttgtcatc
    12901 cgtataataa ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg gctgttgtag
    12961 atgctcaaga tattgggcag attctctgaa taaatcttta tctaatatcc cataacctaa
    13021 cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa ttgtcataac ctaagttttt
    13081 atatacatga tctctattcc aaaaactagc tacattccca tggaaaacag cgcttgtata
    13141 gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag acattgtctg acccaagttc
    13201 tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc
    13261 acttgtcttt ccctgtccta cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt
    13321 ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg tctaccttca tattaatcaa
    13381 aaactgctgg aaactttcta aatggagaac gatcacattt ttccctttag cgatcccaaa
    13441 tgtttcggga tttggctctg cataatgttt ctttgtataa tctaaaactt catcaatccc
    13501 attactgctt gcatgcgcgc gaacactgct agtcattcct gttttgattc cgtcataaat
    13561 agtatacgca tcgattccta gatatttgac aatatatgaa cggtcaaatg ttcgctgcag
    13621 caattgtgga cggttggctt cgctcagcgc tagatttcct gaaaaaataa gccctgaaag
    13681 acaggtaaca gcgatagcca tcggtttgcc gacagggttg cttttgatag gtattttctt
    13741 gaccttcaac caaaccagta gcccaatgaa cacagcgata tccagccagt agatgacatc
    13801 atggggcttc atcaaagaaa aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc
    13861 taatacagat ttgactgtaa tgaaatctgt gaattcccga taaaaaataa tattcaagta
    13921 caacaaaagc gtatttaaaa tatcaatgat caaaagaata gctaacgctg gctttggctt
    13981 tttaatatac aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt tgatccacaa
    14041 gataaagtac tggatcgtac cttccacacc taatgaaaaa tctgcatagt aggcaatgat
    14101 cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt gctaaacgat tttggcacaa
    14161 ttttttaagt tgtgtcacgt atatcacttc tctctattca cattttttct tattttgctc
    14221 ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag atttttttat tctttctaaa
    14281 gattattccg tccacttttc acagcataaa accgctgcga tgtaagcgat taaactaatg
    14341 aagagataaa agtcttgtgg tgaccgcttc actccacggt attcaaaagc cagtttctcg
    14401 gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt atttcttgat
    14461 gcaggacagc tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc
    14521 acaagtcaca atatttataa aacatgagaa gctgtttttt gaaattgttc cttg
//
LOCUS       DACEMS010000064        14375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEMS010000064 DACEMS010000000
VERSION     DACEMS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.065"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            142..523
                     /locus_tag="IW735_001728"
                     /pseudo
     CDS             142..523
                     /locus_tag="IW735_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            654..1604
                     /locus_tag="IW735_001729"
     CDS             654..1604
                     /locus_tag="IW735_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1044729.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2122..2577
                     /locus_tag="IW735_001730"
     CDS             2122..2577
                     /locus_tag="IW735_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1044730.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2888..3244
                     /locus_tag="IW735_001731"
                     /pseudo
     CDS             2888..3244
                     /locus_tag="IW735_001731"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3257..3667
                     /locus_tag="IW735_001732"
                     /pseudo
     CDS             3257..3667
                     /locus_tag="IW735_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3782..3946
                     /locus_tag="IW735_001733"
     CDS             3782..3946
                     /locus_tag="IW735_001733"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044731.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(4097..5076)
                     /locus_tag="IW735_001734"
                     /pseudo
     CDS             complement(4097..5076)
                     /locus_tag="IW735_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5081..5848)
                     /locus_tag="IW735_001735"
     CDS             complement(5081..5848)
                     /locus_tag="IW735_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1044732.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6338..6763
                     /gene="lacA"
                     /locus_tag="IW735_001736"
     CDS             6338..6763
                     /gene="lacA"
                     /locus_tag="IW735_001736"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ1044733.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6780..7295
                     /gene="lacB"
                     /locus_tag="IW735_001737"
     CDS             6780..7295
                     /gene="lacB"
                     /locus_tag="IW735_001737"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ1044734.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7306..8238
                     /gene="lacC"
                     /locus_tag="IW735_001738"
     CDS             7306..8238
                     /gene="lacC"
                     /locus_tag="IW735_001738"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ1044735.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8241..9221
                     /gene="lacD"
                     /locus_tag="IW735_001739"
     CDS             8241..9221
                     /gene="lacD"
                     /locus_tag="IW735_001739"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1044736.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9249..9566
                     /locus_tag="IW735_001740"
     CDS             9249..9566
                     /locus_tag="IW735_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1044737.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9566..11272
                     /locus_tag="IW735_001741"
     CDS             9566..11272
                     /locus_tag="IW735_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1044738.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11414..12820
                     /gene="lacG"
                     /locus_tag="IW735_001742"
     CDS             11414..12820
                     /gene="lacG"
                     /locus_tag="IW735_001742"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1044739.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12843..13115
                     /locus_tag="IW735_001743"
     CDS             12843..13115
                     /locus_tag="IW735_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ1044740.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13136..14035
                     /locus_tag="IW735_001744"
     CDS             13136..14035
                     /locus_tag="IW735_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1044741.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4695 a         2371 c         2771 g         4538 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgatgacact atcgttcttc atcaacgtaa
       61 gttagatttg ttgaaagagc agaaaaaagg atttctacaa aaaatgtttg tttgatattt
      121 taaatagaaa agagagataa agtgaagttt ttaaattttt tgaactataa atatttaaac
      181 tgggtattat atggtattac attatttttt attatttatt acttttttac cgatgatgat
      241 atttttttaa ttttagcaat actttttctt gtttcatcta attttagtga taacttaatt
      301 gaagttctta cagaaccaga aattcggtct atgacggatg aagaacaaca gattattaaa
      361 gctatgattt cggaaaataa gaaagacgta gtagttatca aaaagattag agagtataca
      421 aatttaggtg ttttacaagc caaaaaagta tatgaaaaaa aaaaaaaggg tctataatta
      481 caaattagat aatacattag aagctggaga caagattgtc tagataatgt gtgagataaa
      541 atttttggca ggttatcata cataagacaa aatgttttta aaactgataa cgtatttatt
      601 ccacaacaat tacgcattta tttctgagaa aaatataaag caggaggatt acaatgctga
      661 aaacttattc cttaacaaaa gatacattat tagtttcgtc agaatcaaat atcaaaaatt
      721 cttctgttgt ttatgtttat ggtgacgaac gagaatatat cgaatcttct gaacataaat
      781 atgactttaa aataggcaat atactaacgt ttgatgatcg agttgcatat gacacgatga
      841 tagataagaa cgatgaaaaa agtttgctcg taagtttttt gtttcccgag gtacatgaag
      901 gtgggcatct agataccctg acgacatctg ttgttttctt ggtatttaaa aataagttga
      961 taatttttac aaaacaaaaa ttaaaattca ttcccgaact cttatccaat atggttttac
     1021 gtgatgtcaa tcatttcgtg gaggaagtac ttttaaagat catgcaaaaa gatttctata
     1081 tgatgcttaa cgaccttcgt ggagtcaagg aaaaaattga tgatttagac tcagaaatat
     1141 caacgtccgg ttcactcaaa ccaacgtttg gtgatttatt gactcttcaa aaatacatga
     1201 tagctttgtc aacaacttat aaagctaacc ataaagcgct tgatttcatt aaaaaaaatt
     1261 tcacaactat tgacgatatt gattttagta ctaaaaaaat aattgatgat ttatacgata
     1321 caagtgatac aatggataga atcattttaa gttacagcca atatttggac aaattggaaa
     1381 atatgataaa caatatgatt tcgtatcagc taaacatgat tatgaaaacc ctaaccgaga
     1441 tatcaattgt tttgactatt ccagcaatta tttttggctt ttggggaatc aatgtccatg
     1501 tcccgtttga aaagtcttct tatggtgttt tagccgtatt gattatctct gctatactaa
     1561 gtctaatttg ctggcttttg atgcgacgca aaacatattt gtaaagtatt tgtgataccc
     1621 taatatgatt taaagcagaa tttctttgtt gactttttaa aaggctgatt cctttcaata
     1681 atttaatagc ttcagcggct gctcagaagt caataaattg gactattttt attcagttac
     1741 ttaggcaaca tttgagatac gattgcgatt ataaaaattg atataccaac caatggctgc
     1801 ttggaagact gacatattag cctcgatatc atcgaataac taaatacaga aaaatacaat
     1861 aaatattatc aaactaggga ctatactaga aaacatcagt acattcctta actcagggtc
     1921 tataattaga aaatcgcccc tttgaaaact gattaaaaca gcccccatta tctagtgact
     1981 agataatggg ggctgttttt ttgttaagtg atataataaa ccataaaaga agtttagaaa
     2041 tacggtcctg gtcatcaata aataaacaat ttaattaatt atttatttaa ttatttaatt
     2101 tcagaaaggt atacatgaat catggtgcaa caaattgtcc tacccatcaa agactccagc
     2161 gtcttaaaga tggtacaaga tacacttctc gatagtttcc gtgctggtcg ccgaaattac
     2221 accgtttttc aagttggtaa ggccacgcta ctacgtgtga gtgatgtcat gacattaaaa
     2281 aaatcagatg tctataatcc agacggctct gtcaaaaatg caacctttat tcatgataaa
     2341 aagaccggta aagcaaacac gttatattta aagcctgttc agcaagactt gttgcaatat
     2401 catgattggc tggcccaaga aaatatcaat tctgagtggt tatttccctc gacagcccat
     2461 tatgatcttc atatcactga gaagcagttt cataaggtga tggcgcatgt tggtgatctc
     2521 ttaggaatca acttggaaga caaagagaaa aaattgcgag tcagaaaacc gttttgagat
     2581 atgaacagca tttgccttca cgccttagcg attttaaata atagccttga aagtgattat
     2641 ggaaaagccc tctacgctca atgcaaaatt gacgtagaaa ctatttttga tcgattgaaa
     2701 ggtgtatttg gtatgtgaag aacccatgtc agaggcaaga acgttgcacc tactgacatt
     2761 ggaatgatgc ttatgagcat gaatctaacc aaattatccc ttgaggctag aagaaaagcg
     2821 gaagcttttc aacacaaagc agccaaaaat aaaaaacgcg acgaagcaat cacatttatg
     2881 attattcatc gcgtttttta tatttagagc tagttatttg ctagacccac tatttacaga
     2941 tgaaacgttt atcacctttg gttttgggca tttagtggaa ttagcagaac caggtcatta
     3001 tgacgaaaag tggcaaaatt ggaaacttga atctttgccg atttttcctg atcgatacga
     3061 ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgcagaac ttctcaaaaa
     3121 ggcaaataca attattgttg caacagatag cgacagagaa ggtgaaaata tcgcctggtc
     3181 gattatccat aatgcaaatg ccttttcaaa agataaaaca tttaaaagac tatggatcaa
     3241 tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag gaatgaatta
     3301 ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga tcggcatgaa
     3361 cgcaagccct ttgtatacgt taaatttaca acagaagggc gtacaaggta cattttcact
     3421 aggacgtgtt caaacgccca ccttatacct tatttttcag cgccaggaag ccatagagaa
     3481 ttttaaaaaa gaaccttttt tcgaggtgga agctaagtaa aacaagggtc atttaagggc
     3541 gttctaagcc ccacacagcg ttttaaaacc caagaggagc tttttagctt ttgtttcttc
     3601 taaacaagct aaaataggca atcaagaggg gataattgct gatgttcaaa ccaaagagaa
     3661 gaaaacgaat agtccgagtt cgttttcttt aagtagtttg caatcaaaag tcaatcagct
     3721 ttataaagcg acagcgagcc aaactttaaa agctatgcaa ggactgtatg aagcaaaatt
     3781 attgagttat ccaagaacag atacaccatt tattacagag aacgaatttg cttatttaaa
     3841 agcgaatttt ggcaaatata gcggtttttt aggacttgat cttgaaatgt tgttatcatt
     3901 gataatgata cgttcaaaca acagcaccca aattttgacg aattagtgaa actctatgaa
     3961 aaagacgtag taaaacatgc aacatcctat tctaatcagc tggtaaagct taattaggtt
     4021 atataagctt gaaaataaag ggtttgtcca atatggacaa gcctttttct tatgttttgc
     4081 cacagatttg ccacacttat tgtgtaagag tatcaaggat aggtataatt tgatactttg
     4141 tttttttagt gatgtgcaaa taaatttctg ttacacgact acctctagaa tgccctacac
     4201 ggtcttgaat tgcttctagg ggtattcctt actctgctaa gaaactaata tgcgtatgac
     4261 ggaatatatg ggtcgtaata tgtttattaa aactaagttt attatcaata tcaattcctg
     4321 ctcgttttgg attacctttt cgatttaatt ttccattttc aggtgagttg aggaaattgt
     4381 taaggacact ataatgaaga ggtgttccat tatagtcggg tttatgacga cttttggaag
     4441 taaaaatcca tccattatct ttataatcag gattgaatct catatgttgg tgatttaact
     4501 caatttcctc tttaagaatt tctatagccc ttttagataa ttggacattt cggtaacttt
     4561 gtaagttttt tacattacct tcatagctat tttctgcagc tcctccgtga gtggactgac
     4621 taatgatgat attttgcttt tcaaaatatt gataggcacc aatattttgg agtttcgact
     4681 ctgtaagtca acagggccat tagttagaat acctattggg atgcggtttt tatgtagagc
     4741 cagcagtagt tgatgtatct caggaatcat cgtaatctga tcgagttcat attcatagtc
     4801 agcttggaag tctagagctt ctgtcctaga aatactatcg atgccaaatt cttccatagt
     4861 cttctgacag cgaaaaattc taagctgctc aatactgatg agttttttcg tgtatttagg
     4921 aaaagcaaca tcagaccaat atctgaaacg tttgtaaatg ttatctattt gttggatatc
     4981 aatcgttgga aaacatttct caacgctacg tttaaagggg agttgatggt cataaagagt
     5041 gtcatcaagg tcaaaaacaa tatttttata agtcataatt ctagtccgct tttaaaatag
     5101 tagtgtactg gctaaattcc tctaagtgtt gagggctaat ctggttatct gtaatgatgg
     5161 tatcgagttg atctagatta tagaagttaa agaaatcgta tcgatcgaat ttagtactgt
     5221 ctactaataa gaatttttct acagcattgt ttagggcaag ttgttgaatc acaccttcct
     5281 tgtcactata tgtagcaata gaattatggg taacagcatt tgcactaaca aaagctttgg
     5341 caaatctaat tgcttttaaa tttgtcgaag ccattgaacc tacaaaagct ccagttattt
     5401 ctctatattc accgccaaga agcaataaat caatggtttc gctatcattt agaatcaaga
     5461 acacagggag actatttgta atgacacgaa ttttataacc tttacgacct ttcaattcta
     5521 atgccagttg ttcaagtgta gttccaggtc caataaaaat agtatcgcca tctttgatta
     5581 aagagcaggc ttttttagca atgtcaattt tttcttttgt atttagactt ttcttctcga
     5641 tatgtgtctt ttcaagtggc tttttagagg aaagcaattg tgcaccacca tgagtacgtg
     5701 ttaaaagtcc atcagcttct agagcatcaa ggtctctacg ggctgtcata tcggaaatat
     5761 ctagttcatc tattatttct tttatggtta tggtcccatc aatctttaac atatctaaaa
     5821 ttttttctaa tcgtcgtttt ttgttcatat gaagaccctt ctttcataaa gtaatttttt
     5881 tccaaagata attctctttt aattgtatca taaaagacaa tattttcaag gtaaaacaaa
     5941 caatttcaaa caaaaacaaa tgttagatga tgaaataaga acagaggatt gacgtatatt
     6001 agcttaggtc agattttgta taagacgaaa ataaagtagg acctcttaat cagtaagtta
     6061 tagaaagtaa aagacttttg taatacttga atagatattt cacgtccatt ttgtgatgga
     6121 ttaaatgaac aaaaatgaac aataatttaa cggtgttatc tattttttaa aaaaacaaat
     6181 aaaaaaaaca aaaaataaac aaaaaaagtg ttgctttttg tttgaatgct tgatattata
     6241 taaacaaaga aatgatgaaa acgttttttc gaacattttg caaaatgttt tctacttcta
     6301 cgtagcattt ctttctaaaa tttaggaggt ggtcaaaatg gctattgttg ttggtgcaga
     6361 tctcaaaggt acgagattaa aagatgttgt gaaaaatttt cttgtagaag aaggttttga
     6421 ggtaattgat gtgactaagg acggacaaga ttttgttgat gttaccttgg cagttgcctc
     6481 tgaagtaaat aaagatgagc aaaacttagg tattgtaatt gatgcttatg gagctggtcc
     6541 attcatggta gcaactaaaa ttaaaggtat ggttgcagca gaagtttcag atgaacgctc
     6601 agcttatatg acacgtggac ataacaattc acgaatgatt acggttggcg ctgaaatcgt
     6661 cggtgatgag cttgctaaaa acatcgctaa aggtttcgtc aatggtaaat atgatggcgg
     6721 acgtcatcaa atccgagtag atatgcttaa caaaatgtgt taagaaaagg agttaaggaa
     6781 tgagaatcgc aattggatgt gaccacattg tcacagatga aaaaatggca gtatcagaat
     6841 tcttgaaatc aaaaggacat gaggtccttg atttcgggac atatgaccac gcacgtactc
     6901 actaccctat ctatggtaaa aaagtcggtg aagcagtagt aagtggtcaa gcagacttgg
     6961 gagtatgtat ctgtggtaca ggtgtcggca ttaacaatgc agtcaataaa gttccaggtg
     7021 ttcgctcagc gttggttcgt gatatgacat cagctatata tgcaaaagaa gagttaaatg
     7081 cgaatgttat cggtttcggt ggaaagatta ctggtggtct tcttatgaat gacatcattg
     7141 aagctttcat tgaagctgag tacaaaccaa cagaagaaaa taaaaaattg attgcaaaaa
     7201 tcgaacacgt tgaaacacat aatgcacatc aagcagatga agatttcttt acagaattcc
     7261 ttgaaagatg ggaccgtggt gactaccacg attaaggtat aacaaatgat tctgacagtc
     7321 acactaaatc cttcagtaga catctcttat cctctagaaa cattgaaaat tgatacagtg
     7381 aatagggtga aagatgttag taagacagct ggtggaaaag gcttaaatgt tactagagtc
     7441 ttgtatgaat ctggtgataa agttactgct acaggtttct taggtggtaa aattggtgaa
     7501 tttattgaaa gcgaattaga acaatctcct gttagtccag ctttttataa aatctcagga
     7561 aacactagaa actgtattgc tatcttacac gagggtaacc aaacagaaat tttggaacaa
     7621 ggaccaacaa tttctcatga agaagcagaa ggctttcttg accactatag caatcttatt
     7681 aaacaatcag aagttgtgac aatttcagga agtttacctt caggacttcc caacgattat
     7741 tatgaaaaac ttatccaact tgcttcggat gaaggagtag cagtcgtttt ggattgttca
     7801 ggtgcacctc ttgaaacagt tctaaaatcg tcggctaaac ctactgccat caaacctaat
     7861 aatgaggaac tttcacagtt gcttggaaaa gaagtaacaa aagatattga agaactcaaa
     7921 gacgttctta aagagtcact ttttagtggt atcgaatgga tagtggtctc attaggtcga
     7981 aacggtgcct ttgcaaaaca tggtgatgtt ttctataagg tagatattcc tgatatccca
     8041 gttgttaatc cagtcggatc aggggactca acggtagctg gtattgcatc agctttaaat
     8101 agtaaaaaaa gtgacgctga cttattaaaa catgcgatga cattgggtat gttaaatgct
     8161 caagaaacaa tgacagggca tgtgaatatg actaattacg aaacactaaa ctcacaaatt
     8221 ggagtaaaag aggtataaaa atggtactta cagaacagaa acgcaaatct ttggaaaaaa
     8281 tttcagataa aaacggtgtt atctcagctt tggcatttga ccaacgtggt gctttgaaac
     8341 gtttgatggc acagtatcaa gatacagaac caactgtagc tcaaatggaa gaactaaagg
     8401 ttttggttgc tgatgaactt acaaaatacg cttcttcaat gttgcttgac cctgaatatg
     8461 gacttccagc aacaaaagca ttggataaag aagcaggtct tcttcttgcc tatgaaaaaa
     8521 caggttatga tacatcaagt acaaaacgtt tgcctgactg cttggatgtt tggtctgcaa
     8581 aacgtatcaa agagcaaggc gctgatgccg ttaagttctt gctttactat gatgtagaca
     8641 gctcagatga acttaaccaa caaaaacaag cttatattga acgtgttggt tctgaatgtg
     8701 tcgctgaaga tattcctttc ttcttggaaa tccttgctta tgatgaagaa atctcagatg
     8761 caggttctgt tgaatatgca aaagtgaaac ctcgcaaagt cattgaagcg atgaaagttt
     8821 tctctgatcc acgctttaac attgatgttc ttaaagtaga agtaccagtt aacgttaaat
     8881 atgtcgaagg ttttgctgat ggtgaagtag tttacagtaa agctgaagct gctgacttct
     8941 ttaaagcaca agaagaagcg actaaccttc catacatcta cctaagtgca ggtgtatctg
     9001 ctaaattgtt ccaagaaaca ttgcaatttg ctcacgactc aggtgccaag tttaatggtg
     9061 tgctttgtgg acgtgctact tgggcaggat ctgtagaacc ttacatcaaa gaaggtgaaa
     9121 aagcagcacg tgaatggttg cgtactactg gattcgaaaa tattgatgaa cttaacaaag
     9181 ttcttgttaa aacagctagt ccatggactg ataaagtata gtaaaaacat aaaacggagg
     9241 atattgttat gaacagagaa gagatgactc tcttagggtt tgaaattgtt gcttatgctg
     9301 gagatgctcg ctctaaactt ttagaagcgc ttaaagcggc tgaaaatggt gatttcgcta
     9361 aggcagatag tcttgtagta gaagcaggaa gctgtattgc agaggctcac aattctcaga
     9421 cagctatgtt ggctcgagaa gcttctgggg aggaacttcc atacagcgtt accatgatgc
     9481 atggtcagga ccacttaatg actactgtct tattaaaaga tgtgattcat cacctcatcg
     9541 aactttataa aagaggagca aaataatgca taaactcatt gaacttattg agaaagggaa
     9601 accgttcttt gagaaaatct ctcgaaacat ctatcttcgt gctattcgtg atggatttat
     9661 cgctggtatg ccagtcatcc ttttctcatc tatctttatc cttattgcct atgtcccaaa
     9721 tgcttgggga ttccactggt caaaagacat tgagaccttc ttgatgactc cttatagcta
     9781 ttcgatgggt atcctagcat tctttgttgg tggtactacc gctaaagctt tgacggactc
     9841 aatgaaccgt gacctacctg cgaccaatca gattaacttc ttatctacga tgctagcgtc
     9901 tatggtaggg ttccttttga tggcagctga gcctgcaaaa gaaggaggct tcttgacagc
     9961 cttcacggga acaaaaggtc ttttgacagc atttattgca gcctttgtga cagttaatgt
    10021 ttacaaggtc tgtgtgaaaa ataatgttac tattcgtatg cctgaagagg ttccaccaaa
    10081 tatctctcaa gtatttaaag acttgattcc attcactgta gcggttgttc tcctttatgg
    10141 atttgaactc attgttaagg gaactcttgg tgtaaccgtt gcagaatcaa tcggtaccct
    10201 tcttgctcct ctcttctcag ctgcagatgg ttaccttgga attacactta tctttggtgc
    10261 ttatgccttc ttctggtttg ttggtatcca cggtccttca attgtcgaac cagcaatcgc
    10321 tgctatcact tatgccaata tcgatgccaa cttgcatctt atccaagctg gacaacatgc
    10381 agacaaagtt atcacttctg gtactcaaat gtttattgtt accatgggtg gaacaggagc
    10441 tacattgatt gttccattct tgttcatgtg gatttgtaaa tcagaacgta accgtgccat
    10501 cggacgtgcc tcagttgttc caacattctt tggggttaat gagccaatct tatttggtgc
    10561 accaatcgta ttgaatccga ttttctttgt accgttcatt ttcgctccga ttgtcaacgt
    10621 ttggatcttt aaattctttg ttgacacatt gaacatgaac tcattctctg ccaaccttcc
    10681 atgggttact cctggtccgt tgggaattgt actcggtact aacttccaag ttctatcatt
    10741 tatcttagcc ggactcttgg tagttgttga tactatcatt tattacccat ttgttaaggt
    10801 atacgatgaa caaattcttg aagaagaacg ttctggtaaa acaaacgatg ctcttaaaga
    10861 aaaagtagct gcaaacttca atactgctaa agcagatgcc gttcttggaa aagcaggcgt
    10921 tgctaaagaa gatgtagcag caaacaataa tatcacaaaa gaaactaatg tccttgttct
    10981 ttgtgcaggt ggaggtacaa gtggcttgct tgccaatgcc ttgaacaaag cagcagcaga
    11041 atacaatgtt ccagtcaaag cggcagcggg tagttacggt gctcaccgtg aaatgttgcc
    11101 agagtttgac ttggttatct tggctccaca ggtcgcttca aacttcgatg atatgaaggc
    11161 tgaaacggat aaattgggca ttaaacttgc aaaaactgaa ggagctcaat atatcaaatt
    11221 gacacgtgat ggacaaggtg ctcttgcatt tgttcaacaa cagtttgatt aataaatttg
    11281 taaaagttat ttgagacgag attattcttt caattcctta taatattgtt aaccatatca
    11341 ttgcatttca tttccttggt ttccggaact cagaatatcg tctcaaatgc tttttttgaa
    11401 aggatttata gttatgacta aaacacttcc taaagatttt atttttggtg gtgctacagc
    11461 tgcttatcaa gcagaaggtg cgactcacac ggatggtaaa ggcccagttg catgggataa
    11521 gtatcttgaa gacaattatt ggtacacagc agagcctgca agtgattttt accataaata
    11581 tcctgttgat ttagaattag ctgaaaaata tggcgttaat ggtattcgta tttcaattgc
    11641 ctggtctcgt attttcccaa ctggctatgg cgaagtaaat gaaaaaggtg ttgaattcta
    11701 ccataatctt tttgccgagt gccacaaacg tcatgttgag ccattcgtga ctttgcacca
    11761 ctttgacaca ccagaagttc ttcactcaga tggagatttc ttaaaccgcg aaaatataga
    11821 acactttgta gattatgccg ctttctgttt tgaagagttc ccagaagtaa actactggac
    11881 aactttcaat gaaattggcc caattggaga tggtcaatac ttggttggta aattccctcc
    11941 aggcattcaa tatgatcttg ccaaagtctt ccaatcacac cacaatatga tggtttctca
    12001 tgcacgtgct gtgaaattgt ataaagataa aggttataaa ggcgaaattg gtgtagtaca
    12061 tgcgttgcca accaaatacc cttatgatcc agaaaatcca gcagatgttc gtgccgctga
    12121 gttagaagat atcatccata acaaatttat cttggatgca acctaccttg gacactattc
    12181 agataaaacg atggaagggg tcaaccacat cctagctgaa aatggtggag aacttgatct
    12241 tcgtgatgaa gacttccaag cacttgaagc agctaaagat ttgaacgatt tccttggtat
    12301 caactactac atgagtgatt ggatgcaagc ctttgatggt gagactgaaa tcattcacaa
    12361 tggtaagggt gaaaaaggaa gttctaagta ccagattaaa ggtgttggtc gccgaatcgc
    12421 accagattat gtgcctagaa cggattggga ctggattatt tatcctgaag gcttgtatga
    12481 ccaaatcatg cgagtgaaaa atgattatcc aaactacaag aagatttaca tcactgaaaa
    12541 cggtcttgga tacaaagatg agtttgtaga taataccgtt tatgatgatg gtcgtattga
    12601 ttacgtgaag aaacacttgg aagttttatc agacgcaatt gcggatggtg caaatgttaa
    12661 aggttacttc atctggtcac ttatggatgt cttctcatgg tcaaacggat acgaaaaacg
    12721 ttatggacta ttctatgtag actttgacac gcaagaacgc tatcctaaga aatcagctca
    12781 ttggtataag aaagtagcag aaactcaagt gatagagtaa tattacttga aaatgtaaaa
    12841 aattgttatc cgatacttta gaaaaagtac aacacttatt tagaccaggc tttgaaaagg
    12901 tatatattgt tagtttcgaa gattgtccta tgattcctga gcttgaagct accccgttgc
    12961 taaaatgtgg taaatggtat gtttcaactg gaaaagagtg gatttgccat tctgatttag
    13021 agttatcagc atttgaatgg gaatttttac aatcactcga tgtagaaata cgtgagacta
    13081 ttcacttcga ggtaaattat ttgcctttcc aataataaaa agaaaggaat tagttgtgac
    13141 tatcgaatta aaaaacgagt atcttactgt tcagtttaaa acacttggtg gtcaactgac
    13201 ttcgattaaa gacaaggatg ggatagagta tctctggcaa gctgatccaa attattggaa
    13261 tggacaggcg cctattctat ttcctatctg tggtagttta cgaaatgatt gggctattta
    13321 tagacctcaa gaaagaccct tttttacagg tcttattcgt agacatggtt ttgttcgaaa
    13381 agaagaattt attctggaag aagtcaatga taatagcgtg acctttagta ttaaaccaaa
    13441 tgctgagatg cttgataatt acctatatca gtttgaactg agagttgttt ataccttgaa
    13501 tggtaaatca attagaaccg aatttcaggt aacgaacttg gaaatggaaa aaacaatgcc
    13561 ttactttatt ggagcccatc cagcattcaa tagtccttta gtagaaggtg aaaactatga
    13621 ggattattat attgaattta gtgaggttga gtcttgctcg ataccaaaga gttttcctga
    13681 gacaggatta ttagatttgc aagatcggac accttttttg gaaaatcaaa aaattctcga
    13741 tttggactac tcactttttt cccatgatgc cattacactt gaccagctca agtctcgtag
    13801 cgtaacgctt cggtcaagga agtctggtaa aggtcttcga gtagattttg atgattttcc
    13861 gaacttgatt ctgtggtcta caactaataa aagtccattc attgctttgg aaccttggag
    13921 tggtctctcc acttcacttg aagaaggtaa tttcttagaa gataaaagac aggtaacgaa
    13981 aatttcacct caagaaactt ctagaaaaat ctatgatatt actatcctta attaaaattt
    14041 gttattattt gagtcttcaa taagatttga atactcttac cttgtgatga tagattggtc
    14101 ttctccgtgc cagtcttaca tcgcaaggtt tttaagtttt ttgtgggtct atgtcaactg
    14161 tagtgggtga cgaaaagcta atatctaaag acagtctggt agtaaaaaat aagattattt
    14221 actacttctt gatttaaaat aatagtggag ccattaattt gtggcaaatt taatttaata
    14281 ttttgcctca aattagccac actttttata gaactaagag gaaaaatgaa attttcttcc
    14341 tattaatata gttgtttgaa atcttaaata tagcg
//
LOCUS       DACEMS010000065        14293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEMS010000065 DACEMS010000000
VERSION     DACEMS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.066"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            133..2604
                     /locus_tag="IW735_001745"
     CDS             133..2604
                     /locus_tag="IW735_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1044742.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2707..4443)
                     /locus_tag="IW735_001746"
     CDS             complement(2707..4443)
                     /locus_tag="IW735_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1044743.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4440..5144)
                     /locus_tag="IW735_001747"
     CDS             complement(4440..5144)
                     /locus_tag="IW735_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1044744.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5275..5778
                     /locus_tag="IW735_001748"
     CDS             5275..5778
                     /locus_tag="IW735_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1044745.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5738..6076
                     /locus_tag="IW735_001749"
     CDS             5738..6076
                     /locus_tag="IW735_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1044746.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6097..7515
                     /gene="ffh"
                     /locus_tag="IW735_001750"
     CDS             6097..7515
                     /gene="ffh"
                     /locus_tag="IW735_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1044747.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7627..8205
                     /locus_tag="IW735_001751"
     CDS             7627..8205
                     /locus_tag="IW735_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1044748.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8209..8730
                     /locus_tag="IW735_001752"
     CDS             8209..8730
                     /locus_tag="IW735_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1044749.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8809..9363)
                     /locus_tag="IW735_001753"
     CDS             complement(8809..9363)
                     /locus_tag="IW735_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1044750.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9616..9891
                     /gene="rpsP"
                     /locus_tag="IW735_001754"
     CDS             9616..9891
                     /gene="rpsP"
                     /locus_tag="IW735_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1044751.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9903..10154
                     /locus_tag="IW735_001755"
     CDS             9903..10154
                     /locus_tag="IW735_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1044752.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10279..11070
                     /locus_tag="IW735_001756"
     CDS             10279..11070
                     /locus_tag="IW735_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044753.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11240..11761
                     /gene="rimM"
                     /locus_tag="IW735_001757"
     CDS             11240..11761
                     /gene="rimM"
                     /locus_tag="IW735_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1044754.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11751..12512
                     /gene="trmD"
                     /locus_tag="IW735_001758"
     CDS             11751..12512
                     /gene="trmD"
                     /locus_tag="IW735_001758"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1044755.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12648..12995
                     /gene="rplS"
                     /locus_tag="IW735_001759"
     CDS             12648..12995
                     /gene="rplS"
                     /locus_tag="IW735_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1044756.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13253..13546
                     /locus_tag="IW735_001760"
     CDS             13253..13546
                     /locus_tag="IW735_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1044757.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4794 a         2567 c         2905 g         4027 t
ORIGIN      
        1 tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt
       61 tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat
      121 cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa
      181 agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat
      241 caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa
      301 acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc
      361 ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc
      421 atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa
      481 ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata
      541 gatatagaag accttgtaat agatgacatt gttattttag agactggata tcaaattcca
      601 gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa
      661 gcagatgaaa taaccaaaga aataggtgat ccattaatgt ccggcagttt cattgtttcc
      721 ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca
      781 gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa
      841 ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt
      901 tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc
      961 ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact
     1021 aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg
     1081 gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa
     1141 aaaatgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc
     1201 attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca
     1261 tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa
     1321 aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt
     1381 ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat
     1441 cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca
     1501 gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca
     1561 tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc
     1621 accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca
     1681 tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc
     1741 gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct
     1801 atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg
     1861 atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac
     1921 ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga
     1981 tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg
     2041 atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact
     2101 atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa
     2161 ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc
     2221 ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca
     2281 tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg
     2341 aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc
     2401 tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc
     2461 attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg
     2521 tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta
     2581 tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac
     2641 cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc
     2701 tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc
     2761 tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc
     2821 ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg
     2881 ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc
     2941 tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc
     3001 aatcagattt ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt
     3061 tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat
     3121 ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt
     3181 tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat
     3241 ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt
     3301 aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc
     3361 cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg
     3421 tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc
     3481 tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg
     3541 aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac
     3601 gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat
     3661 caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt
     3721 acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc
     3781 ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt
     3841 ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat
     3901 cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact
     3961 ctcagttctc ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg
     4021 gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt
     4081 gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt
     4141 tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat
     4201 taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc
     4261 tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta
     4321 gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc
     4381 aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt
     4441 catcgtttgg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg
     4501 tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg
     4561 ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca
     4621 cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc
     4681 agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat
     4741 agatattcag gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt
     4801 gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca
     4861 cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc
     4921 ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga
     4981 agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg
     5041 cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc
     5101 gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa
     5161 taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag
     5221 aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt
     5281 ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta
     5341 ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc
     5401 ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg
     5461 tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa
     5521 aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg
     5581 aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata
     5641 aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc
     5701 tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg
     5761 aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat
     5821 ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag
     5881 ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa
     5941 aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc
     6001 atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca
     6061 agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa
     6121 cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta
     6181 aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta
     6241 gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt
     6301 ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt
     6361 tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt
     6421 cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat
     6481 gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa
     6541 ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc
     6601 ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta
     6661 ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa
     6721 atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa
     6781 gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc
     6841 actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc
     6901 gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca
     6961 gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg
     7021 cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca
     7081 ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa
     7141 gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta
     7201 gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga
     7261 gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac
     7321 agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag
     7381 caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc
     7441 aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag
     7501 aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg
     7561 gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg
     7621 gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact
     7681 gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt
     7741 aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca
     7801 tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta
     7861 atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc
     7921 aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt
     7981 tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt
     8041 cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc
     8101 acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt
     8161 ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc
     8221 atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa
     8281 tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc
     8341 gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa
     8401 gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca
     8461 gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa
     8521 ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa
     8581 atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg gattccttta
     8641 ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa
     8701 tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat
     8761 gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa
     8821 tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc
     8881 aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg
     8941 ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa
     9001 gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact
     9061 gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg
     9121 gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg
     9181 agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc
     9241 atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc
     9301 tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt
     9361 catacgattt ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga
     9421 gcaaataaaa acacttactg acaaaagcta catagaagat agattactta tttttaacaa
     9481 aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga
     9541 aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg
     9601 aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt
     9661 ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa
     9721 actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta
     9781 gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca
     9841 aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg
     9901 atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg
     9961 atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg
    10021 ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca
    10081 caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg
    10141 ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt
    10201 tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga
    10261 ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta
    10321 agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct
    10381 gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca
    10441 atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca
    10501 aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca
    10561 catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat
    10621 gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct
    10681 tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa
    10741 gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt
    10801 gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat
    10861 cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct
    10921 gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta
    10981 agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg
    11041 aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt
    11101 aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct
    11161 taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa
    11221 aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca
    11281 aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa
    11341 aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa
    11401 aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa
    11461 tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct
    11521 agctgaaaat gaattctatt accatgagat catcggtctg catgtattag aagatggaac
    11581 tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca
    11641 acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga
    11701 tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg
    11761 atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta
    11821 aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata
    11881 acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag
    11941 tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa
    12001 gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt
    12061 cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc
    12121 gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag
    12181 gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga
    12241 cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc
    12301 cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga
    12361 ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt
    12421 tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag
    12481 aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg
    12541 ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg
    12601 ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa
    12661 tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca
    12721 ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag
    12781 gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt
    12841 ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg
    12901 aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg
    12961 gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
    13021 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
    13081 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
    13141 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
    13201 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
    13261 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
    13321 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
    13381 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
    13441 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
    13501 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
    13561 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
    13621 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
    13681 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
    13741 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
    13801 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
    13861 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
    13921 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
    13981 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
    14041 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
    14101 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
    14161 aagcaatact tcaattaatc aagttttatc tttttttttc aataatcgta aaaaaagagt
    14221 attttttatt taaaaataaa atctttgtat taaaggacaa gactaaaata accttagaat
    14281 aaaaagagaa aaa
//
LOCUS       DACEMS010000066        14195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEMS010000066 DACEMS010000000
VERSION     DACEMS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.067"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1127)
                     /locus_tag="IW735_001761"
     CDS             complement(<1..1127)
                     /locus_tag="IW735_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1044758.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1210..1545)
                     /locus_tag="IW735_001762"
     CDS             complement(1210..1545)
                     /locus_tag="IW735_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1044759.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(1555..1929)
                     /locus_tag="IW735_001763"
     CDS             complement(1555..1929)
                     /locus_tag="IW735_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1044760.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(1942..2256)
                     /locus_tag="IW735_001764"
     CDS             complement(1942..2256)
                     /locus_tag="IW735_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1044761.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(2369..5596)
                     /gene="fss3"
                     /locus_tag="IW735_001765"
     CDS             complement(2369..5596)
                     /gene="fss3"
                     /locus_tag="IW735_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1044762.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            6384..6668
                     /locus_tag="IW735_001766"
     CDS             6384..6668
                     /locus_tag="IW735_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1044763.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            6671..7588
                     /locus_tag="IW735_001767"
     CDS             6671..7588
                     /locus_tag="IW735_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1044764.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            7581..8363
                     /locus_tag="IW735_001768"
     CDS             7581..8363
                     /locus_tag="IW735_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1044765.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            8364..9044
                     /locus_tag="IW735_001769"
     CDS             8364..9044
                     /locus_tag="IW735_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1044766.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            9037..9933
                     /locus_tag="IW735_001770"
     CDS             9037..9933
                     /locus_tag="IW735_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1044767.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            10128..11777
                     /locus_tag="IW735_001771"
     CDS             10128..11777
                     /locus_tag="IW735_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1044768.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            12371..13306
                     /locus_tag="IW735_001772"
     CDS             12371..13306
                     /locus_tag="IW735_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044769.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            13299..14069
                     /locus_tag="IW735_001773"
     CDS             13299..14069
                     /locus_tag="IW735_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044770.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         4184 a         2724 c         2831 g         4456 t
ORIGIN      
        1 tgatcgcgga ttccgtctcc taaatatttc gcatccggcc tttggcaagc caaaatcaga
       61 aagaaaccag attgacgacc caacatcacg atttgtttga gcttattcaa aatcaccgca
      121 ctttctttcg tcgttaacat ctccatataa gccacgtatt catcaaagat taaaaagttg
      181 ggtggaagtc caagataggc ataattctct cctgttttgt agttagacat ttctttcatt
      241 gccttgctac gagccatcat acgttcatag aaatcttcca cacaagcaga aatttcttcc
      301 ttttgtgaat aaacatgagg catcaccgta cctaaatcag ctaaatctgc atttttggga
      361 tcgaggataa acaattctgc atctgacttc aatagtgctt cgataatggt gaggaggaaa
      421 taggtcttcc ctccacccgt gccaccagcg attaacatat ggggtaaaga atcataagcc
      481 catacttgat ttttcatcaa tcgcagagtc ccattttctg ccactacttc gtctatccca
      541 attcgattgg cgatcatatc atacagtaaa gtatactcca cataggagtc tttgagttcc
      601 ttttctacta actcacaata cagtccgctt tctaacttct tttccaattt taaaagctga
      661 tcttggtatt tcccaagtga aatttgaacg cgaatagaca gtaatccatc tttcattcga
      721 taatagattt tggggaagta actgatagtc tctctggttc ggctactatt caaatctttg
      781 aaaaagcctt cactttgagt ttgtttcact tcataccaat ggttctctag aatcatccga
      841 gctagctttt gtcgatgcca caagcttcgc caatgatcaa gaaaaaagtg aatataaagc
      901 aatatcatag ctcctgaaaa agcgagactt ccaagcagac atactaaatc ttttatagac
      961 caaacgattt caatagtaga aagtgatagc ttagaaatat caaattgtct aaactcttga
     1021 aaataaaaga cgctcatcca accgatgaac agagcgagta gccagccaag aacaaagtga
     1081 taaaccaagt gctgatctcc tgcgcgtatg cgatgtcctt tgtacatttt gatggtttcc
     1141 acctccaatc gaaaaagccc aataggattt cattaggcaa tttcaaaaag ttatgctagt
     1201 catttactgc tacgaggagg ggagttgttt cgtttcaacc aattgattta aaaagccttc
     1261 aatagcatcc ggcatctcca caccaatttc ttccaaaatg gattctgcct gccgacataa
     1321 gttttcattt acttcgacaa ctataaagaa agactcttct tctcctaacc agtctagtac
     1381 atcagagtct tcttcactgt cgctattttc tccaagtaaa ccttgatgta taatcagaaa
     1441 cattttttca tcagaggtta atgctttaac gagttgttcc tcagtcagct cgccttttcc
     1501 atattgatcg agacaatcta aaatatgttc aacattaatt tccataatct tcacctattt
     1561 ctttctttca ttttggttcg taggatttcc ttgattcggc tgccgcttca agactaaatc
     1621 atccgcctta atgaaccaat taacggtagt tccttctcga aaaacataat tggcaacggt
     1681 atccacgatt ggattgacca actctacctc tgcatcataa gcaaactcgc gtaagggtac
     1741 attagctggg atactaactt gaatcattcg cccttgtgca gaggatttta aattataggt
     1801 tcgttctttt acctcattac caattgttcc atcctcattt tgcttccgta cttctcggcg
     1861 taatgcggaa aaacgcaact tcccaaatgt taaacctgaa tctacgacaa taccttctgt
     1921 taatctcatc ttcttttcct cctatgcttt cactaaatca tcaacataca acacgtaatc
     1981 agtaaaccca cgattctcaa tctcatagcc tttggcaacc aaatggggat tcacggcttt
     2041 tagcggttgg tcttgatcaa aatccttttc accggcttcc gcagctacta ccacctcaat
     2101 atcatcggcc cgttgcttac tggaatacaa atgataactg cgaccaatca ccgtagtatt
     2161 ccgtccatat ccttctgtta taatttctcc ttcaccggca taacttaact ttccaaaggt
     2221 ttcagccata tctggcacga caaatttcaa ttccatcttc ttttcctctt ttcattgtta
     2281 gttttggata caaaaatagc gcgactactt ttttaggagg cggtcgcgca cgttggttct
     2341 taggatttac ttgtatcatc tcatcacctt agttccggcg attccagaaa taatagcccg
     2401 ccgtcgcaaa aatcaaagta aaaccaatga acagtaaaac attggaattt ttctccccgg
     2461 tttgagggaa ggtcttcact gtgggactag attcttgaga gccacttgga acagttggtg
     2521 tttccggttg tttcggcgta cttagtatgg ttggaacttc ttttggtgtt aaggtttgag
     2581 atttttcttt caaatcttca ttgtgttttc cattgatatt cccatcttta tcgtaattga
     2641 tttccgcaaa agtaaacttg gttccttctg gatattttcc ggtatctact tttttcgcaa
     2701 gcacagtttt ggtgaattct ttatcattta cttcataatc aatcttgcca gatttccaaa
     2761 tttctttgtt tgtaccatcc ggtaataaag catacagaat tgtttcgaaa gcttcttttg
     2821 agccatccag tacatcatga gtaatcgaca catcatcaaa catatccacc acatcaccat
     2881 gagtaaaggt ctgagaaccg ttttctaggt gggcctttgt ttggatagaa acatggtgtt
     2941 ccactgtaca actgacagtt tgggcttggt tattcaagct aacatccttc gctactggct
     3001 tatcgcctgc ttcaaaagct tccttatttt catacacata gttgaataag acaatagttt
     3061 tatttaaaac ttgttcggcg gttaatttat gctgaatttt ccaagttcct gttttctctt
     3121 tgttcgccaa cgttgcgctt gtttctgcaa tcaccactgg tttggctttt tcatcttttt
     3181 cttgggcagc ttttgtcgca tccacatcaa tggcttgcgc taccacatac cagtcttctt
     3241 tgatttcatg tagattgtaa ctcaatttat caactagctc ggtagaatcc agagaagtta
     3301 attctttgtt tccgtctttc caagtcgcaa gagaagttaa actatcttct tcctcaggca
     3361 aatcatagag cattaaacgg ttcaaactaa cagaaaatgc tttgttcgtc agcttctcgt
     3421 aaggaacggt caccgtttta attggttgtt tttggtcttg ttcggtaatg gtaaagacat
     3481 actcactctt cacaaagtct tctttgtttt ccttaaaagt agaacggatt tctaatggcg
     3541 taatcttttg aaaaccttct ggggcttcta cttcttcaag taaataatcc ccataaggaa
     3601 gattttcaaa cttgccataa ccatcaaacc ctaattgctc gttataagct gtggtcgctt
     3661 catcttcggc acctgtgatt tcattggtcc cttccaatgg cgacacttta aaggtcagat
     3721 cattgaagcc agtttcggta gtgcccgcag ctgatccagc aaatttaaag aaatcaaagc
     3781 caaagcgaat cacttgttct tttgccgtaa catttcgggt aatcacggca tttttttcgc
     3841 tatcatccac ctttttgatg gatacaggat actttgtttc atcaaggcta tatccttcgg
     3901 gcgcttttgt ttcttgccaa taatactcgt tgatagctaa atgtttaacg gcaacttggt
     3961 tcttttcgtc taaagtcaaa gtgaccgttt catcagaacc tttcgttccc ttcgctaact
     4021 ctggtttaaa agcctcactc catttaacag cttttccatc cttagcagta aacaaggtgt
     4081 attcagcacc ctcaaagact gccttacctt gtgctttatc cccagtatct ttgtcttctt
     4141 tcgtcaaagt ggtttcgcca gtgacttctt gattttgccc ttttacgtta ctggtaacaa
     4201 gggctacggt ttgattggca tacttcaatt cgacttttac aggtttgaac gtattcacga
     4261 acccatgact agctttagtt tcggtcacat aataggttcc caattccaac gcattagcaa
     4321 tctcttttgg tgtttccgca tgaccgtttt catccgtggt catttcttga acgatttcac
     4381 cggttggact gtctttgcga atggcaaacg tattaccagc taaggaataa ttgtcattcc
     4441 aaagatcact ccctgtttca actccgcttt tatccagaat gatttgccct ttttcccgtg
     4501 cgttttttga agtcacgtaa atggtttcgc ctgctttaat ggtggtagtc atgggggtgg
     4561 tatcaatcgt ataaggcgcc ggtactgatt tttcggtaat ggttactttt gtaccatgag
     4621 gaatctcatc caatgtagca atgccttctt tgtcagtagt cacgtctttt gcaggtaaag
     4681 tttttccaaa gtctaaatgg aaaaccgttc ctggtacaac atcacccgat tctttatcga
     4741 cttttttgat ctttaaagaa ccctccgttt ccacatcaat tttgattgtg taggtattag
     4801 gtttatcaat cgccccggcc attaaagtct gttggccagc ctttttatag gcgacaggtg
     4861 ttccggtacc agcagatttc ttaagcgtca gcacaccact ttcttttgaa ttggcatttg
     4921 gggtgattat caactgatta ccattcacac gataatcaat gtttgcggca ttttccacaa
     4981 cctcatcgaa ctctgacaaa ttcagattat tcgtatccgt cacagtagtc gattgcccta
     5041 atactgtttt agccgtacta ttatggaagg ttggtttctt ctgataatcg tcaatggctt
     5101 gatttatttt tgcctcaatg gctgcgatat ttacggcact accatctgat cgttttattg
     5161 agtgaagggt atacccatta acttcttgcc agatcatctt ctgtgccaca atatgagtat
     5221 ctgcatctgt acctactttt ttccataaaa cggaaactaa tttggcttta tcggacatat
     5281 ctggtaacgg gtttttctca tagccaggag tgaactcatt gtaaatcggg actcctggtt
     5341 caatacagaa aatatcttgt ttaaagccgt tgtacactgc ataatgcgga acagtgactt
     5401 ctgacattgg acgaccatca ctaaaagttc cttcaacagc atattgatgt gctaaatcta
     5461 gttctacggt taccgtttgt ggatgggtaa tttcatcagc aaaagtaatc gtaggagcaa
     5521 ttgctccaac aattgcttga acagccaaac tcaaaatagt taatacacat aatttctttt
     5581 tttgtttttt atacatgaaa tgttctcctt tcaaattaag cacaaaaaaa taagatgctc
     5641 gcttgaacat ctttgtttat aaagtctttt taaaattttt attgactgaa tagaatggat
     5701 ttgagaatga atgagcctaa aaatttaatc taaaatacga aatatactgc ctttaaatta
     5761 gaacacattt tagaacacaa acatttcaaa tttaatcaaa tataataaaa tatatttacg
     5821 ggaaaacctt tataaatcaa ggtttttcaa acttattcaa tgatacgaag aaaaaaccag
     5881 gtcttaagaa agcccgtaaa gcttcacagt tctcaaaacg ttaattgcga ttatcgttcg
     5941 agttcacgag ttacgagaat cgtactgggc gaagcaatgc tgagcgcagt taccttaatc
     6001 ctaacttttc tggcactttc cacaatttgg aaagtgcctt ttttgtgttt ttagctatgt
     6061 taataggtag gattcttggt ctataatatc attcgatcta taaacaaacg ggaaaaagat
     6121 aaagtatcat caaacaatat tatttatccg cttctgaggt tgtgacagaa gtggtcagct
     6181 tcaagaaata agaaggaatt tacaaaaatt gcttctcatg tttgataaat attgtgactt
     6241 gtgaccgagg agttggcagg agaacaccgt ttattcggaa gaagggattg tgaaaagtct
     6301 tttgtcataa cctcttattt ggtagctaat tgtaaatccc aaacggcatg tattattttt
     6361 cataagtccg tttttaagga ttaatgtttt cttttgcttt ttatatggtc tggcttcagc
     6421 gagaagttcc aacgcatata caaggacgtc tgatgtcaac gataacaacg attaccatgg
     6481 tggccagtcc acttggatta gcactgtttg gtgcgctctt tgattggcag acaactcaac
     6541 cacgtctgcg agacagtctg atttttcttg aaattcaatt ggaaaattta cactttcttt
     6601 atacatttct ggctgctttc tttatacccg tcaactatac ttcacttatc aataagaaag
     6661 aaggataagt atggattttg gaaatgaaac ggtcttgaaa gcaactggta taaccaaaaa
     6721 atacggtgct gccaaagcat tagataaagt atcgatcgaa atcaaacgtg gaatgatcta
     6781 tggtttgatt ggggagaatg gagcgggtaa gtctaccttt atgagaacta ttatgggttt
     6841 gatctcgatt gatgaaggtt cgatcgaatt atttggaacg acggatttgc aggcagcaag
     6901 acgacgtatg ggtcaatcga ttgaaacccc tgctctttat ccagaattaa ctgcaagaga
     6961 taacctacgt atccaagcag cgaacggcgg tgtgtcggat agagaaatcg aagacctgct
     7021 gaaaatgatg cgattggaaa atacagggaa aaagaaagca aagaatttct ctttaggtat
     7081 gcgccagcgt ttagctattg caaatgcact gatcacgaat ccagaatttt tgattttgga
     7141 tgaaccgacg aatgggatgg atccagcagg gatggcagaa atgcgagaaa tcatccaacg
     7201 tttggtaaaa gaacggggca tcaccgtctt gctttctagc cacttgctag atgaattgtc
     7261 acagatcgcc actcactacg gtattttgca tgaaggacac ttgatcaaag aattatcgaa
     7321 agaagaattg gcacaagaat ctcgtcaatt tataaaaatt gacacttcgg cgacggaaca
     7381 agcagtaacg gttctcgact ctcttggtta tagggattat tttgtccaat ctagccgtgt
     7441 tatccagctt tttgaaggaa ttgaccaagt agcagccatc aatcaagcat tggtagaggc
     7501 aaaagttcct gtggatggta tccacctagt tgggcaaaaa ctggaagatt atttcttaca
     7561 attaactgga ggcaatccac atgtttaatc tattaactgc tgaaaaaata aaagtatgga
     7621 agagccgtaa aatgtgggtc tcactaggac tgatggtcgt acttccgctt ttttattctt
     7681 ggaacgaatg gtatatgcat acaaaatacg gcaatgaatt aaaccaagca acggatacag
     7741 tcatcaacgg tgcaacaggg attttaatgg tggaaaaaat ggctgcatgg attcttctgg
     7801 ctttcgcagc atttgcgtgc ttttatattg gggaagaatt tcaaaacggt acgattcgca
     7861 atacgttatc tttaggacgg aatcgaatga cttattatgt gtcgaaactt ctcgttacgc
     7921 ttttgatcac aacacttggc gtggttttga ttacggggct agccatgatt gcttacacac
     7981 ttgctttcgg atttggtgaa gttgagggaa tcaaaaacta tggaaattat gttctaaaag
     8041 tatttcctgt attgcttctg ctgatattag cgacattatc tgttccagtg gctttgacat
     8101 tcatcactag aagtaccagt gtcagtttgc ttctttcttt tctttatatc atgggaacgg
     8161 ccttcgttcc cggtgttttc gcaaaaatca aaggcttgga atttctaacg gagtggttta
     8221 cggaaacgtg gctcatgtat acggactttg cgcaacaagc aacttattcg caagcaccga
     8281 aaatgattct ggtcagtcta gtaacaatcg tggtatcatc agctctagga atgtttattt
     8341 tccaaaaatc tgacattaag taggtgttcc tgctggaaac tattttgatt atcgaagacg
     8401 atgaagctgt tcattcttta ctggaggagg ttcttgaaca gagatacaaa cttcttgatg
     8461 cttattcagg tacggaagga aaattattgt taagcacata tcctgtcgat ttgatccttc
     8521 tagatctcat gcttccaggg ctttcaggtg aagcattgtt agcagaaatc cgacagacat
     8581 cgaatgttcc gatcatcgtt ctttcagcaa aaagcgatca aagagataaa gtttccttgt
     8641 tggcagcagg agcggatgac tatgtgacga aaccatttga tatcgaagaa ctgttgttac
     8701 gaatcggcat ccaacttcgt catcagactt ctgtccaagt aaaagatctg tcgcaacgca
     8761 tctattacaa ggaaatctta ctggacaaag agtcgaggga tgtaaatgtg aatggtgtgg
     8821 ctgtccattt gacaggaaga gaattcgatc tgctgaagct ttttttagaa catccgaaaa
     8881 aagtattttc gcgagcaaat ctttatgaaa cagtttggca agaaccatat tttgatagtg
     8941 agaaaacagt gaatatgcat atttccaatc ttcgagggaa actagcaaag gcacatgctg
     9001 tgtatattca tacggtgtgg ggagtggggt ttagatttga ttaagtggct gttgcttctc
     9061 gttgttatct ttatgggaag ttttattttc ttcctgcaaa gggaaatcaa gagattaact
     9121 aagaaaatcg ccactctttc tgaacgacaa gaatatggtg gtcgtctttc agtcgaattt
     9181 cgagaaaaga atctaatgga cctagtagat gcactgaata catgggtgga tgaaaatgaa
     9241 caaaaaggac aagcatttca agaaatggaa gaaaacatca gactatcgat cgtcggcctt
     9301 tctcatgatc tcagaactcc tttgactgct attaacggtt atgtacaatt actggaacag
     9361 gtagaggata cggagaaacg ggagaagtat ttaacgatca tcaaacaatc tgttgcaaga
     9421 ttactcagta tgacggatca gttttatgat cttgctcgaa tagagacgaa gcaaaaagaa
     9481 atggatctcc atacgctttc tttttctaaa gaggtggaag aagtattttt ctctttttat
     9541 gaacaatttt ctgagaaaaa tatcgaggta gcgttcgctg aacactcgat aaatacaact
     9601 gtattagcag atccgctcat gttgctgcga gtgctgcaaa atatcataca gaatttattg
     9661 cgttatgcag atggacaagc aaaggtgaca ttttcaaaag aggattcatt cattcgatta
     9721 acagtggcca atcctatcaa accaggagcg caaacctctg ttgaaaaagt cttccagcgt
     9781 ttttataccg aaaataccag ccgcacaaat acggaatcta gcggaatcgg gctttacctg
     9841 tcaaaaaaat tggtggaagg aatgggagga gagatgaccg ctagattaga cgggggcatc
     9901 ttctccatct ccgtcaagtt gcggagggtt taattggctc taatttacct gaagtgaatg
     9961 acaaaaaatt gtatctcgtt ttaaagacat cttctttttt ggaaggtgtt tttattttgg
    10021 tgtgattgat acgaattagg taacgaagac agttattttt gtaccccctt ttcaaatagc
    10081 gcaacagtgc tatactaaaa ctaacaaatc aaggaacgga gagggatatg ggccaaagag
    10141 aatttatcgt tttaattatt attgcagttg ttattttatt agttgtttta gacatttttt
    10201 cacgtttgaa agtaaaggaa acagttcgta gtaattgggg aaaaattcct tatcaaccga
    10261 gatttgataa agaagaaagt ttgaaggatg cgtggctcac ggagaaaaaa ttccgttcat
    10321 gggatagtga aatcgacgat cttacttggt atgacttgga tatgttcgaa gtatttgaag
    10381 ggatcaacag tacctattct agtgtaggtt cagaagcgtt gtatcagcgt ttacgtagct
    10441 ttgattttgg agaagattat cagttagaaa aactaattgc tttttatcag gaaaatcctc
    10501 aactgagaga aagaatccaa tatcaattcg ctcgtttagg aaaaaaagat cataattttg
    10561 caaaacaata tctagcggat ggaaaaagta aaaagattgg acgcgtaggt ttatttattt
    10621 ttcttggctt gcttccgctg gtgggtgtag gtttgcttct tttagggcaa gtactaggaa
    10681 tctatctagc aatcggtggc ttagtattca atacggtcta ttatctagtg aagaaacaaa
    10741 cactagaaac agagctgaat agcatgagtt atttggtttc aacgatttca gccgccaatc
    10801 agttagcaaa aatgacgaca ccacttcaaa aagagctgaa acagcaaact aaaccattaa
    10861 gagacatccc taaattaggt ttttcatttc gaatgaagag cgggagtgaa gcagaattga
    10921 tttttgaata tgtcaacatc atgttcatgc tgccatttat ctcctatcat ttcgttctca
    10981 ctaaattaga aaagcattca actgaagcca tcaagctgtg ggaattgctg ggagaattgg
    11041 aagttgcagc agccgttttg aattatcgca cctatatgcc gatcacttgc cgaccagagt
    11101 ttactgaggg cggtgtgaca gcgaaagata tctatcatcc attgttaaaa gaagcggtag
    11161 ttaatccagt caactggcag aagaatacat tagtcacggg ttccaatgca tcaggaaaat
    11221 ctacgtatgt caaaagtata gccatcagct gtattctagc acagacgatc caaacagcga
    11281 ttgcagaaca gtttaccctg caacctggac atgtgttgac ttccatggca atcgaagatg
    11341 atttatttga aggcgacagt tattttgttt cagaaatcaa atcgatcaaa cgtttgctgg
    11401 accaagtggc ttccaaagaa cgctgctact gcttcgtaga tgagatttta aaaggaacaa
    11461 atacgatcga acggattgct tcttccgctt cagtcgttcg gtggcttgcg gagtatccat
    11521 ctttagcatt tgtcgccaca catgatatcg aattgacaga gattttaaag aattattgtg
    11581 agaatgtcca ctttgaagaa caagtaacag aaggaaaagg aatcagtttt gatttcaaat
    11641 taaaagaagg accttcaagg acccgaaatg cgattgcctt gctgaaagtt ttgaattatc
    11701 ctaaatccat agttgaacag gcccaaaaag aaagtttgtt gttcgatgaa cagcgtcagt
    11761 ggtatccgtt tgattaactt tatcaaacga agcatgatgc gtgggtccaa aaagacgatt
    11821 atcaaaagaa gtacattgat gccaaaaaag tggcagaatt caatgggaac gagtctgctg
    11881 tccagatgat cttggaagat ttagcaaaag acgatacaaa ataaaccacg ctgtcttcct
    11941 tctagcagct tttatcgaaa aaacttagca tcagcgacga gacgcacgtt ccatggcaaa
    12001 gttctccgta aaacaaacag cgaaaacaga aattgaaaat aactatttcc attttattta
    12061 tttcggttaa aataaaagaa aaaagctttc gataaaagga cggaggaaag cttaacatga
    12121 ataaaaaaga caagaacctc agttataagg atcttctata tgtcatcgta gtagttgctg
    12181 tcggtacttt tatgatttta aaagttccag agttgtttag atagttattc tatcgaagat
    12241 gatcatgaac tagaaaacac atctcctctt tgttagcagg tgtgtttttg tgtggaagcc
    12301 aagttgtttt ttcatctgct gtattccatg atacaattaa gaatcatgtc tataaggaga
    12361 taggaaataa atggcaaatg cgcttgaaat aaataatttg aaaaagatct atggaagtgg
    12421 ggtcgaagca ttgcggggaa tcgatctttc ggtgaaagaa ggagatttct atgcgctatt
    12481 agggccaaat ggagctggga agtctacaac gatcgggatt attacttctt tagtcaataa
    12541 aacatctgga aaagtgaatg tcttcggtta tgatctggac aaggatctag ttcgagcaaa
    12601 gcaacagatt ggattggttc cacaagagtt caattttaac ccttttgaaa cggtccagca
    12661 aatcgtcgtg aatcaagcag gctactacgg tgtctctcgc aaagaagcga tgaaacgcag
    12721 cgaaaaatat ttgaagcagt ccaatctttg ggaaaagaga aatgtccgtg cacgtatgct
    12781 ttccggggga atgaagcgaa gactgatgat tgctagggca ttgatgcatg aaccacgttt
    12841 gctgatcttg gatgaaccaa cagcaggagt tgacatcgag ttgagacgcg agatgtggga
    12901 gtttctacga gaattgaacg aaaatggtac aacgatcatt ttgacgacgc attatctaga
    12961 agaagctgaa atgctttgtc gcaacatcgg aatcatccaa tcgggtgaac tgatcgagaa
    13021 tacaagtatg aaagagcttc tgtcaaaact gcaatttgaa acatttatct ttgatctagc
    13081 gccatatgat accaaacctg tgattgaagg atatgcttgt acatttgaag atgagcagac
    13141 aatcgctgtc gaagttgaac gaaatcaagg ggtgaacgga atcttcgatc agcttacaag
    13201 acaaggaatc aaagttcttt ctatgaggaa taagtcgaat cgtttagaag aactgttctt
    13261 gaaaatcaca gaagaaaata atcatacgga ggagaaaaat gtttaatctt tattttactg
    13321 cattaaaaag tttagctatc aaggaaacga accgctattt gcgtatatgg gtccaaacac
    13381 tggttccgcc agtgatcacg acatccttgt attttgttat tttcggaaaa atgatcggtg
    13441 ggcggatcgg tgacatgggt ggtttttcat atatggaatt catcgttcct ggtttgatta
    13501 tgatgtctgc tatcactagt tcgtatgcca atgtttcttc gtccttcttc tctcaaaaat
    13561 ttcagaaaaa tatcgaagaa atcttggtag cacctgttcc tacgcatgtg attatttggg
    13621 gctttgtgat tggtggtgtg ggtcgcagta ttctcgtcgg ctctctcgta acgatcattt
    13681 ccttgttctt tgtaccactt catgtgtatt catggttcat ggtcatcatt acattattga
    13741 tgacggcaat cttgttttca ttagcaggac tgctcaacgg gatttttgct cagtcttatg
    13801 atgatgtatc gatcgtgcct acatttgtct tgcagccgtt aacttatcta ggtggcgttt
    13861 tctatgccat ctctatgtta ccgccctttt ggcaagctgt ttcaaaagtc aatccgatcg
    13921 tttatatgat ctccggtttc cgttacggat tccttggaac gatcgatgtg ccagtagcaa
    13981 catcgatgat cgtacttgtg ttattcatcg ttgttttgta cgccgttact tggtatttga
    14041 tcaataaagg aagaggattg agaagttaga aaagaggttg tgaaatcagc gttttgacct
    14101 gagtattaga gaaacaaacg ggaaaatagt tcctcatatt ttgttgtttt ttgagcgaat
    14161 accgagggtc agctgattga acaccgttta gtgga
//
LOCUS       DACEMS010000067        13897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEMS010000067 DACEMS010000000
VERSION     DACEMS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.068"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..362
                     /locus_tag="IW735_001774"
     CDS             168..362
                     /locus_tag="IW735_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044771.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IW735_001775"
     CDS             631..930
                     /locus_tag="IW735_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044772.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IW735_001776"
     CDS             complement(1027..1461)
                     /locus_tag="IW735_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1044773.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IW735_001777"
     CDS             complement(1693..2403)
                     /locus_tag="IW735_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1044774.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IW735_001778"
     CDS             complement(2393..3253)
                     /locus_tag="IW735_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1044775.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IW735_001779"
     CDS             complement(3258..3896)
                     /locus_tag="IW735_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1044776.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IW735_001780"
     CDS             complement(3911..4210)
                     /locus_tag="IW735_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1044777.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IW735_001781"
     CDS             complement(4253..5737)
                     /locus_tag="IW735_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1044778.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IW735_001782"
     CDS             complement(5757..6218)
                     /locus_tag="IW735_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1044779.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW735_001783"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW735_001783"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1044780.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IW735_001784"
     CDS             7521..8291
                     /locus_tag="IW735_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1044781.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IW735_001785"
     CDS             complement(8564..9391)
                     /locus_tag="IW735_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1044782.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IW735_001786"
     CDS             9959..11194
                     /locus_tag="IW735_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1044783.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IW735_001787"
     CDS             complement(12193..12453)
                     /locus_tag="IW735_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1044784.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IW735_001788"
     CDS             complement(12480..13781)
                     /locus_tag="IW735_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1044785.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4478 a         2837 c         2180 g         4402 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatc
//
LOCUS       DACEMS010000068        13885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEMS010000068 DACEMS010000000
VERSION     DACEMS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.069"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            144..2309
                     /gene="acm"
                     /locus_tag="IW735_001789"
     CDS             144..2309
                     /gene="acm"
                     /locus_tag="IW735_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1044786.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2581..3066)
                     /locus_tag="IW735_001790"
     CDS             complement(2581..3066)
                     /locus_tag="IW735_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1044787.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3546..4187
                     /locus_tag="IW735_001791"
     CDS             3546..4187
                     /locus_tag="IW735_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1044788.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4206..4841
                     /locus_tag="IW735_001792"
     CDS             4206..4841
                     /locus_tag="IW735_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044789.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4859..5674
                     /locus_tag="IW735_001793"
     CDS             4859..5674
                     /locus_tag="IW735_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1044790.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5993..7429
                     /locus_tag="IW735_001794"
     CDS             5993..7429
                     /locus_tag="IW735_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1044791.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7618..10065
                     /locus_tag="IW735_001795"
     CDS             7618..10065
                     /locus_tag="IW735_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1044792.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10295..11362
                     /gene="rlmN"
                     /locus_tag="IW735_001796"
     CDS             10295..11362
                     /gene="rlmN"
                     /locus_tag="IW735_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1044793.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11555..12658)
                     /locus_tag="IW735_001797"
     CDS             complement(11555..12658)
                     /locus_tag="IW735_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1044794.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13211..13759
                     /gene="aac(6')"
                     /locus_tag="IW735_001798"
     CDS             13211..13759
                     /gene="aac(6')"
                     /locus_tag="IW735_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1044795.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4758 a         2300 c         2939 g         3888 t
ORIGIN      
        1 ttatttttta taaatatttg taaaataact atcaattttt tagttaaaag attggaaatg
       61 agtgggggag gaattgaaga aaggattttt gagagatgat atagtaggtt ttatttttag
      121 ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa tattatttta tcaatgttat
      181 ttattattac taatataaca tcactgattc ctgttcatgt ctatgcggat gcaggcagag
      241 atatcagcag taatgtcact tcgttgaccg tggacccaac gaatattaca gatggaggaa
      301 acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc
      361 tttggatcaa ttggccaagt gaaggaaata ttcgcggtga aggattccaa aaagaaatcc
      421 cattaatgat tgaaaataaa aatgttggaa ctttaacggt cagaaaagat tctgcgcagg
      481 ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga aggttggggg caatttgaga
      541 tacaggccag aaacgtaacc gataccggtg aagaaaatac gggaccattc accgtgacga
      601 gcggtgataa aacagctact gtaaatgtga caaagccagc ttctggttct tcttctagcg
      661 ttttttatta taaaacagga gacatgctac ctgaagatac taagcacatt cgatggtttt
      721 tgaatattaa caataacggc acttatgttg aacaaccagt taaaatcagt gatgaaatcc
      781 aaagtggaca aagattagat ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac
      841 agaaggttta tagaggagaa gagggaatcc aacaattttt acaagatttc cccagcgcta
      901 catttaattt ttcagtaact gataattaca ttgaaataac tataccaaaa aactttgtca
      961 atcttaggaa aataatggtg agttataaga cgatcataga aaacccagaa caaataaatt
     1021 ttgagaatca ttcggaagct tggtttaaag aatttaataa acctgctgtt gatggagaaa
     1081 gtttcaatca tacagttaaa aatatttcag cttctggtgg agtcaacggt acagttagag
     1141 gcgaattgaa gattttcaaa tatatcaatg acacagagat tggtatccca aatgtgactt
     1201 tcgaattaag acgagcagat gaacagccta ttcaaggaca gtctagtatc ctgttgactt
     1261 cgaatgaaca gggagaagct agtatcaaag gcctgcaagt aggagactat gttgtaaaag
     1321 aaaaagaagc acccaattgg attgattttg atccactaag ttcaaatgaa ctcaaattct
     1381 ccataaacga aaatgataca gagggagtca gtttacctat ttataacaag aaaaaggtta
     1441 cgaacatcac agcaactaaa atttggaacg gtggaaccac tccaagaccg agtatttatt
     1501 ttaaattatt cagagcaagt acaaacaatt gggaaccagt tcctgatgca gaaacaaaac
     1561 gattagacaa tggaataact tcagtgactt gggaagatat ccaacagtat gatgatagtg
     1621 gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt
     1681 caggttatca aaaaattgaa aacgggctag tcgttacaaa tgagaataaa gaagtcattt
     1741 cagtctcagg acaaaaaaca tgggaagaca atgagaacca agacggcaaa cgaccgacga
     1801 cgatcacagt aaatctatta gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa
     1861 aagatgattg gcgctatcgt ttcacaaatc tgccaaaata cagagacggg caagaaatca
     1921 tctatactgt aacggaagac aacgtgcccg aatacagtac gacgattgac gggtataaca
     1981 tcaaaaacag ttatacaccg gaaaaaacaa atatatctat accaggtagc catataacac
     2041 cgtggaaaaa ttttccagag aagacagaga gagagaaaat tagaaatcta ttctctcaaa
     2101 aattgcaaat atttgctcat acaggagaat ccagcgaaca aagaagcaat tatattagta
     2161 agagagctgt acctaaacag ataacaaata aatcaaaaag cattcttcct aaaacaagta
     2221 gtaaaaaatc tagttttgct gtaatcattg gtttattgat agtaactatc tgtgctggga
     2281 tattcttaca aaaatataaa aaagtttagt tagaatataa tattttttgt taaatatgac
     2341 tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt ttatatatgc
     2401 ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa tactcgtatt
     2461 agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat
     2521 attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca
     2581 ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga
     2641 aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg
     2701 atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa
     2761 tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa
     2821 agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc
     2881 aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac
     2941 ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac
     3001 tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg
     3061 actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg
     3121 aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag
     3181 ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa
     3241 cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag
     3301 ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa
     3361 aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat
     3421 ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac
     3481 cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa
     3541 agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat
     3601 tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag
     3661 gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc
     3721 ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga
     3781 tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt
     3841 ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc
     3901 aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa
     3961 agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga
     4021 tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg
     4081 cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa
     4141 caatcataat gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa
     4201 gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag
     4261 atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg
     4321 gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt
     4381 atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag
     4441 tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag
     4501 caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag
     4561 cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt tctggcggac
     4621 aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg
     4681 atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat
     4741 cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa
     4801 atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat
     4861 gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc
     4921 gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat
     4981 aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg
     5041 atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca
     5101 gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg
     5161 gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat
     5221 ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat
     5281 gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca
     5341 accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa
     5401 tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata
     5461 cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc
     5521 ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa
     5581 aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa
     5641 atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg
     5701 tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag
     5761 ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt
     5821 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga
     5881 aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg gaaggggttt
     5941 cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca
     6001 caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca
     6061 agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg
     6121 gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg
     6181 ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt
     6241 agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt
     6301 ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata
     6361 tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat
     6421 cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca
     6481 ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc
     6541 acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt
     6601 agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc
     6661 aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc
     6721 tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata
     6781 tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg
     6841 cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac
     6901 ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg
     6961 aaaaaaaggt tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc
     7021 atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact
     7081 gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga
     7141 gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca
     7201 aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg
     7261 cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt
     7321 tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag
     7381 ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt
     7441 aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc atttagaaat
     7501 taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa
     7561 ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg ataataaatg
     7621 gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa
     7681 cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt atttgctgtc
     7741 ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt tcggatcatt
     7801 gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt ttggattgtc
     7861 attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg catactcttt
     7921 tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca tactggggaa
     7981 ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca aagtcaagta
     8041 gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac ttacttcttg
     8101 attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg agcattcctg
     8161 ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg agaacgagta
     8221 caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc aaaggaagag
     8281 agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca
     8341 gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     8401 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     8461 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     8521 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     8581 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     8641 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     8701 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     8761 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     8821 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     8881 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     8941 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     9001 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     9061 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     9121 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     9181 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     9241 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     9301 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     9361 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     9421 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     9481 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     9541 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     9601 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     9661 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     9721 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     9781 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     9841 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     9901 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     9961 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
    10021 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
    10081 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
    10141 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
    10201 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
    10261 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
    10321 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
    10381 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
    10441 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
    10501 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
    10561 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
    10621 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
    10681 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
    10741 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
    10801 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
    10861 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
    10921 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
    10981 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
    11041 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
    11101 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
    11161 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
    11221 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
    11281 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
    11341 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
    11401 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
    11461 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
    11521 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
    11581 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
    11641 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
    11701 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
    11761 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
    11821 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
    11881 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
    11941 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
    12001 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
    12061 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
    12121 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
    12181 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
    12241 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
    12301 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
    12361 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
    12421 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
    12481 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
    12541 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
    12601 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
    12661 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
    12721 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
    12781 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
    12841 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
    12901 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
    12961 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
    13021 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
    13081 tttttatttt ataagtaaga taaataatga aagaatagag atactgattg gtagctaaag
    13141 atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc aagaacaagg
    13201 gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga aagatcagct
    13261 ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg cagaagaagt
    13321 agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag atgagttagt
    13381 aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc atccattagt
    13441 tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt acttagaaaa
    13501 agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt tagaccatgg
    13561 aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg cttccatcca
    13621 gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt
    13681 cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa cgattattcc
    13741 tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata
    13801 agaaggaatt tacgaaaatt acttcttgaa tttttgtgaa attccaactt atttccgaag
    13861 aagctacttt tggaacaccg tgtac
//
LOCUS       DACEMS010000069        13884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEMS010000069 DACEMS010000000
VERSION     DACEMS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.070"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..319)
                     /locus_tag="IW735_001799"
     CDS             complement(26..319)
                     /locus_tag="IW735_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1044796.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(347..1012)
                     /locus_tag="IW735_001800"
     CDS             complement(347..1012)
                     /locus_tag="IW735_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1044797.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1152..1784)
                     /locus_tag="IW735_001801"
     CDS             complement(1152..1784)
                     /locus_tag="IW735_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1044798.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1791..2858)
                     /locus_tag="IW735_001802"
     CDS             complement(1791..2858)
                     /locus_tag="IW735_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1044799.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2855..3586)
                     /locus_tag="IW735_001803"
     CDS             complement(2855..3586)
                     /locus_tag="IW735_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1044800.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3754..4233)
                     /gene="greA"
                     /locus_tag="IW735_001804"
     CDS             complement(3754..4233)
                     /gene="greA"
                     /locus_tag="IW735_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1044801.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="IW735_001805"
     CDS             complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="IW735_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1044802.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5709..6869
                     /locus_tag="IW735_001806"
     CDS             5709..6869
                     /locus_tag="IW735_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1044803.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6909..7856)
                     /locus_tag="IW735_001807"
     CDS             complement(6909..7856)
                     /locus_tag="IW735_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1044804.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7989..9380)
                     /locus_tag="IW735_001808"
     CDS             complement(7989..9380)
                     /locus_tag="IW735_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ1044805.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            9506..9916
                     /gene="tagD"
                     /locus_tag="IW735_001809"
     CDS             9506..9916
                     /gene="tagD"
                     /locus_tag="IW735_001809"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ1044806.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            10096..11385
                     /locus_tag="IW735_001810"
                     /pseudo
     CDS             10096..11385
                     /locus_tag="IW735_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11452..12879)
                     /locus_tag="IW735_001811"
     CDS             complement(11452..12879)
                     /locus_tag="IW735_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1044807.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(12876..>13884)
                     /locus_tag="IW735_001812"
     CDS             complement(12876..>13884)
                     /locus_tag="IW735_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ1044808.1"
                     /translation="EKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLY
                     TAKYWIDDSRKTIKYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESI
                     TYLLSGNRYSSKKYVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPT
                     FRNNIEDNGITQLEWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKK
                     HQITLINDGIPLEMLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYI
                     QLSELPIISVQDSYGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDI
                     K"
BASE COUNT         4530 a         2664 c         2060 g         4630 t
ORIGIN      
        1 aaaacttgtg tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca
       61 ggttgggatc ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct
      121 cgataaaaaa aagtgtccca tccatttcca ctttctttac aggatgttct ggctgttcaa
      181 ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa
      241 aacgtattcc gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg
      301 gcgttaccgt taattccatc ccaacaacct actttctctt ttctttctat tgattcatct
      361 tatcgtctag aatatccaca tcgcctgtct ctccaatcac cagtagatta tcattttcgc
      421 gaacatattc atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga
      481 ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt
      541 taggattgga gactcggatt tctgctaaag aatatttatc tgacagctct aagtagtcta
      601 gaatattctt tgatactaag ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta
      661 cgacacgatc tgctccgatc ttctctagta cacgggcatg gtactcattt tgggcttttg
      721 ctaaaatatt cggtacgccc atttccttta ccatcagagt aactaagata ctggcttgga
      781 tatcttctcc aatagcgaca attacatgat cgaaatttct tatacctaat gaacgaagtg
      841 tcatctcatc ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga
      901 ctcgatcttc attactgtca atagccagta cttcttgtcc agcttcgatc agtgtacggc
      961 agatgcttcc gccaaatcgt ccaaggccta caattgcaaa attttgtttc accattttcc
     1021 tcctttagta tcttcttttc tcagtataac aaagcctttc caaaaacaaa agcaagcatc
     1081 attattgtaa aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt
     1141 tatcatcgtt tttatttcac caaaccatgt ttaaacgcat agatagctgc ttgagttcgg
     1201 tcttctacct ctaacttagc taatatattc gacacatgcg ttttaactgt tttcagtgtg
     1261 ataaacaatt catccgcgat ttcctgatta cttttccctt cagaaatcag catcaaaatt
     1321 tcattttctc gattcgttaa ttcttcatgc aatactggat catttcgccg actcattttt
     1381 tccatcattt ttgttgtcac ttcaggttcc aatactcgct cgccgcgttg cgtagccctt
     1441 atagcatcag ctatttcatg tgcggtagac gtcttcaaca aatatcctgc tgctcctgct
     1501 tcaattgcag gataaacttt ttcatcatca ataaaacttg tgacgataat gattttggct
     1561 tttggacaat cttttaagat tgcttttgtt gattcgatgc catccatttc ttccatgact
     1621 agatccatta aaatgacatc tggtcgaagt gccatcgcct tttcatagcc ttgacgtcca
     1681 ttttcagctt ctccaatcac ttcaatgtct tcttggatag ataaataaga agagacgcct
     1741 aagcgtacca tttcatggtc atctactaat aaaactttta tcatctttat ctatgcctcc
     1801 ttcatcaaag ggaccttgat ctccacgctc gtaccttgcc ctttaaaact aatgattttt
     1861 actgttccac caataccagc tactcgttct ttgatattgt tcaatccata acttcccgtt
     1921 ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga tacgtaataa aagattgtta
     1981 tctattttat gcaaatagac ttctaattca ttagccttag catgtcttaa tgtgtttgat
     2041 aacaattctt gaacgattcg gaacaagtga tcctcaatgg aactagttaa ttttacatct
     2101 tctacatccc attttagcga aatctggatt ttattctgca attccttcaa taactgctca
     2161 atgccttgtt ttaaactttt ttcttctaaa ttgactggac gcagatgaag aagcagcgca
     2221 cgcatctcag actgggaagc gttgatgatc tctgccacca ttttcaactg cttacggaac
     2281 atttctggtg tctcactttt ttctgcttgc tccgtcaatg ccgacatcat catcatcgct
     2341 gcaaataatt gctgtgaaac tgaatcatga agctcacgag ctaatcgatg cctttctaat
     2401 tctaaaatct cttctttggt ttgtccatct acatattgag gacggctagt cataatctgt
     2461 aattcactgg atacttcgat cattttttct tttattttag caatttccat atcaatatct
     2521 ttgatgtaca gatcatccga cgcgcttggg atcggctgat gcaaaagttt ggattcataa
     2581 ttgccagaag aaagtgcacg aagcttttct tcgattttcc cgtattttgc cttttgaata
     2641 agtgataaca atagaaatac gatcaatcct acacctaatg aaattgccat caaatggaag
     2701 ataagcggaa cgtataataa acgcgcacgc agcaattctc cccaccaatg actttggtta
     2761 ctggcataaa aataagtgaa aagtgaaaat agaataataa ggaaagccgc aatccccgaa
     2821 taaacagcta gcattgcttt ggatattctt cccatcatat tcgtatcacc tcgacatctc
     2881 caaccaaggt attggtaatg atcttcaatc tgcgaggatt ttcatcataa tcactgctgc
     2941 agattttgat ctgttcgttt tttaaggaaa actgctcttc ttcaaacaag acatttccta
     3001 ctaatgtcgc atgctctaaa cgaatagcca ctcccagggg tactaagatc cgtgttctgc
     3061 ctatcccttt tcttacaatc acaatattat catcttttgg caaaagtgta ttacccaaat
     3121 cgatgatagt atcaccagaa atgatggcaa tgttgatatc atcccattca tagacgtcgt
     3181 ttccaatacg ttggttacca aataattgct gtcttttacg ctcattatta tgtgttttta
     3241 attgctctgt ttggaccatc atgatctgtt tttttcgcca aaaagcattt tttgtcaaat
     3301 ctattcctga aatctctaca cctttcagcc cgataaataa aacggccaat acaaccatca
     3361 tccataaagc aggactgttg actaagctaa aaaaaatgac caagctccct aagatcaatt
     3421 gaaaattttg aaatttagta cgaggatatt tgcgcatagc tagatatata ttgaaaatac
     3481 cgaatattac taaaagcaaa agtccagtat tgtttacgat ttgccaaaca gcaaataaca
     3541 gcagcagtgc ttcgactacc acaaaaaaac gccaagaact attcatcgct ttttctccct
     3601 cctgacacat tctcatttca tcatacctat ttttttgact gttggctata ggactttaga
     3661 tgtaagatcg ttcagtgaaa cctgcaatac tcaaagagaa aagatctaga gtgcgtcatc
     3721 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
     3781 aactaacata tcgccacctg gtgtagcaat ggttacttca tcatttttac gtttgccaat
     3841 caatgcttgc gcgataggag aatcattgga aatctttcca gaaaacgggt ctgcttcagc
     3901 acttccgaca atcgtgtact cttcttcatc tccatcaggc aactcgatga acgtaactgt
     3961 tttaccgatc gatacctcat ctgcatctac gccgtcatta tcgatgattt gtgcaaaacg
     4021 gatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttagctga
     4081 ttcgtattcg gagttttcgg aaagatctcc gaaaccgcga gcgattttga ttcgttcaat
     4141 gatttctccg cgtttaactg tttttagttc ttctaattct ttttctaatt tttctttacc
     4201 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
     4261 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttctta
     4321 aaatgtaaat gtttttctaa aaatgatcaa tgactagccc ctgtttttct aagaattatt
     4381 tacatatttt tgcactagtt ccatatgttc ttcatacgtt ttagcgaaat aaacatttcc
     4441 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
     4501 gattgccttt tcgcttggat tgtcaaaagg accaggccca tagccggtat tggtatacaa
     4561 attgtatggt gaatctacag ctgtatcttc atacgtcacc aattctttat gttctcctaa
     4621 tgcatataaa acagagatat ctgactgaag tggcatattt gccttgattc ggttaaagaa
     4681 gacttgtgca atatttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
     4741 caaagttaaa acttcttgta cagtcaaatt tttctgtttg atagctgaat aatagttttg
     4801 catgaccgta tttgtcttgt tcaccatctc aatcacaaga tccttaagcg aagtactcgt
     4861 gtagtaatca taagtagctg ggaataagta gccttccaaa cggtattttg tgttctcagc
     4921 tttagaggca ctttccaaca attctgggaa tttttgatac aactcgttaa agaaagtctc
     4981 atcatttact aaatctaaaa attctttctt gtctttacct gttaccttcg ctacccgctc
     5041 agcaatctga tcgatgtcgt agccttctgg aatggcaatt ttcccatctg caacttttgt
     5101 tggctcagat gtgccgcctt cttgcaactg cttgccaatc tcatcaagag tcatattagg
     5161 cgctaactga taataaccag cttgaaagcc agttaagttt ttaaattttg tataataatt
     5221 gaaaacaatc ccacttttaa taatattgtc tttctccaaa atttcgccaa tctgcttatt
     5281 agaagagccg tttgggattt ccacttggac taattggtcg tccgatttat ctaaaggctt
     5341 caacccgcta tctacataac gatatacggt aaacccgaga aatccgccaa taatcaataa
     5401 tgtaagagcg atcactaaaa tgatcttcct cacgatctta tcttcttttt tacgcagatt
     5461 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tgtctttgct
     5521 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcatcttc gtaactctgt
     5581 ttattataca tgaaatttct actgccgtga attattttac caaatattat gtcaaaattg
     5641 taagtggcaa atgctctttt tgaggattac aatgaaaaat agttaaaaat ggatagaaag
     5701 gatttaatat ggcaaaagga aaacatcaaa aaaaacagca gatctctctt tcttttctcg
     5761 cagttttgtt gctggctgtc agcttctttt caacaggcat cattacagga tggcaattaa
     5821 caaaacaacc tcaaccacaa atttataaaa cggaacccgc acaacttgct tccaatttaa
     5881 catctaatga gcagatgctt gccagcaaaa ttgatcaatt actgcaaaaa tcagattata
     5941 tcggttcgat ttatgtacgc aaaaataatc gagtgatatt agaaaaagga tatgggtacg
     6001 caaatctacg cataaaagaa tcgaattctc cttctcttta ctatcaaatc ggttcgatcc
     6061 aaaaagcgat gactgcttta cttatcctaa aacaaattca atctggcaag ataacttttg
     6121 acacaaagct atctgaattt tatcctcaga ttcctggaag tcagcaaatc tcaatcaaaa
     6181 atatgcttta catgcgctct ggtcttcatc gaaccgcttc tccaaaaaaa cctatgtcag
     6241 atagtgagat cattcagttt gcccttcatc atttgaaatt tactggttat gatacgtatc
     6301 attatgaacc attaaattat acattgctta caggggtatt gatcaaacta atgaatcagc
     6361 cttatgaaac tcttttaaaa aaagaaatca ttcggccact ccatctagaa catacagatt
     6421 tttatgaaaa tgtaaaaaat tcccctcagc atgctgtatc ttaccaaatg tctgcaactg
     6481 acgattattc aaaagcattg gtcgagcctg aaacagacat ccgaaatgaa ttaggcacag
     6541 gaaatataag tatgtctgtt tatgacttga acaaattctt taccagtgtt ctgtctggcg
     6601 atattatacc aaaagaacta cttttctcat tgtggcaaaa taatggtgat aaacatcctt
     6661 acagcggcgg tgtttacagt ggaaatgatt atatccttgc tcagggaaat atcaatcgat
     6721 tccatgcagt agcggcgatg aaaaaagaca caaaagatgc aatcgtcatg gaaagtaata
     6781 tccaatctga taaaaacatc aaattgccgg ccactgattt aagaaatcag atttacgaac
     6841 tgatggaagg tgttaattta aaaagctaag tcgagacaaa aaaaccagca ggatgctggt
     6901 tttttgtttc aatcgatttc tgtctttaaa taacgttcca ttccgttcaa tatatcaaat
     6961 ttgggttgat aacctaaaga acggatcttt gaaatatccg ctaaagaatc acgaatatcc
     7021 cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg atcaattgaa
     7081 tgaatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg ctgtcctaaa
     7141 gctttttctt ccttagcaac aagtaaaagt gcttgtataa catcttctat ataaacgaag
     7201 tctctagatt ggctaccatc accaaataaa gtaaattctg ttttttctcc tgccaattgt
     7261 tttttgtatc ggtccactag aatcgaaata actccgctat atggagagtt aggattttga
     7321 tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata taaatgacag
     7381 taatccaaaa catattgttc tgatgcaaac ttatcgatgg catatggtgt taaaggacgg
     7441 atgaccgatt cttctttttt tggcaacgtt ggttcatcgc cataaactgc agctgatgaa
     7501 ctaaatacca atcgttttaa accagattga tattttcgta ccaattcaag caataaaagc
     7561 gcactgtcaa aatttacacg atgagtttct agaggacgtt ctacagaatc tgccacacta
     7621 gctacagcag ctaaatgaaa aatataggca aactgatact gtttcatgat tctttccatc
     7681 aattgaggat ctgatacatc cccctcaatg aaagtcactt gtttctccat atccaagttt
     7741 tctttttttc ccattgataa atcgtcgact actacgattt tgttttcttt tcccaaataa
     7801 ttcgctagcg ttgaaccgat aaaaccagct ccaccagtaa tcaatatagt atccatcaaa
     7861 ttcactccaa ttatgcattc tcatacttaa taaagttagt ttaccatttt tatattgtaa
     7921 aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt atatacctaa
     7981 aaatagtttc aattagttac tccttcttct ttagatatag ttttttggat agaacccaga
     8041 tatccccaaa aaataaatga acctatcgta ctaaccaaaa tgagttctgt gataaataat
     8101 gccgaaactg ctattgtaag aatcattaaa atagcaaaaa agtgcttatc ttttactaga
     8161 tttggttgtg tgaaacaata acgcaaagtt ttaaagcatt ggataaataa gaaggttaac
     8221 aatgtcaata gacctaatcc tcctgtagca gccataacta agaaaataaa attatgagca
     8281 gttaaagtat cttttcccat taatgtatat ggtagtttat catgtgcgta ttcaatatag
     8341 tatcctggag aaccgccgga tatcggctta ctcttgaata tttcaattgc acttttccaa
     8401 agtccaaacc ttaaattaga aacatccccg ccatcagtgt cagggcgttt taaatcaact
     8461 ttattgctgc tagttgttcc tgatgaatca gatatatctt tttttatttc tgtaatatgc
     8521 ggaacatgtg cccatgcttg agcagttata tctgttacat aatacatcgc aattatactt
     8581 agcaatccta ctactaatgc agttagccat ctttttaaag tattagtttt aagaaaacga
     8641 ctactataac ttgtaaagaa actaaaaaca aaagttgcta ttaataaaca aactaatgtg
     8701 gtgcgtgagc ccgataaagc aataaacgag aattgaataa aaatattaat tgcaaaaaat
     8761 attttaaata gaagatttaa atgcttgtaa gtgaccaaat aatatatagc aataatgatg
     8821 acaattaatg atataaccgc accaaaattt ggatctgtat atacaccaaa taaacggttg
     8881 cctaaccacc ctaaacgaac tgggtaatac ttatatttta ttggaatttt tatgccatat
     8941 tgaatgacaa accagtataa tgatactaaa ttagctaaaa accataccaa tgaaagtatc
     9001 caagtaaata ctttgaaaaa aaagttacta ctgtagtctt tgccaaactg ataaatcata
     9061 aagaaaaaga ttaattgcca catgattaac ttgatatttt cgctatatcc atattttaaa
     9121 ttcattaaac tgcttactag catcgcgcca ataaaagcaa ttaatagcca atcatatttt
     9181 ttaagatttt tttctctaaa tgcaataatt gcatctataa tgatcaagac aactcccgct
     9241 attccagcag gccaaaaaag taaagtatca tatctagtag gtaatagtaa atacgggcct
     9301 attgctcgta acattagaat aaaaccaaaa taaataaaat accaaccttt cacatatttt
     9361 aaaaattttt cattcaacat ttttcctcca tccacctatt tctcataaac atttcttatc
     9421 ctttttagga taaactggtt ctttcaattc taacaaaaaa atgctaaact aacaatgtat
     9481 attaatgaat ttcgagaggt gaaagatgaa aagagtaatt acttacggta cctttgattt
     9541 gcttcactac gggcatatca atttactgaa acgagcaaaa caatatggag attatcttat
     9601 tgttgccctt tctacagatg aattcaattg gaatgcgaaa caaaaaaaat gttattttag
     9661 ttatgaaaaa agaaaacagt tacttgaagc tattcgctac gttgatttag ttattcctga
     9721 agaaaattgg gaacaaaaag taaaagatat aaaagaatac catgcagatt atttcgtgat
     9781 gggcgatgat tgggctggac aatttgatga acttgaacaa ctaacagatg cacaagtaat
     9841 ttatttacca agaacacctg agatctctac aactcaaata aagaacgaac ttaattcttt
     9901 aaatagtgat aaataatatt ttttaaacaa tgaaagaaca atatatctgg ataagacgta
     9961 aaaaaatggc tctataattt ctaggactga tagaattttc tgtcgatcct agattttctt
    10021 gtaaaaaaaa cagacataca aaaatttcga taaaatagag tcgtctaaaa catcaatctg
    10081 aaaggaagat tttgtatatc catttctcgt tctatcaaag agcacttcac tattaaagat
    10141 aaaaatattg aaattaattc ttttcaaaaa actttttata aggacaagaa agccatcgta
    10201 atcgaaggta cattagttcg cacttttaga cattgtctat tttgtaaaga aactgcgaag
    10261 cagctcgtga aaaacggcaa gaagatttca atgattatct taaatcgcag tgggaataaa
    10321 taaacctatt tgcgtcttag aaaacaacat taccactacc aatcatgcgg aaaatatttt
    10381 actgctcata cgtatcttgt cgttccttca tgttttattt cgaaacaagt tcattacacg
    10441 atattagaag agttgaccga gcgtcaagcg ttaaagacac ttacgaaacg ttgtgatctt
    10501 tcagtaacga catttcaacg gacattatct gctctatcgg caatgctgcg acagatgcta
    10561 gactatttac ctcattgttt actattcgac gagtttcgct ctctttcaat ttcccatgga
    10621 aagttctcct tttcttgtat gaatagagag acagggaagc tctttgacat tttgtccagc
    10681 cggagaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc tcatatgggg
    10741 gttcgataca ttgtcacaaa tatgaatgcc ccttattttt cattagtcaa agagtccttt
    10801 ccaaacgccc aaatcatcat tgattgcttt cacattggac aacacttgaa caagtttttc
    10861 gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aagacacttc acaagctaag
    10921 tactatcgac aattgaaacc tttgtataaa ttacttctaa aatcagatga cgagttggat
    10981 tacacaacat tcaagaaatg gcagaatttt caatggcgct atttaacaga aagtgaagta
    11041 gttgatcgtc taccctcaat ctcttatgaa cttaaaattg ctaagcagtt tatcaaaagc
    11101 tgcttagcaa ttatcacaag aaacagctag ctacttcgag aaacgaccaa agaagttccc
    11161 tcgaaacttc actatctcaa aaagcctttt tcatatctga aaaaggcttt taactagcat
    11221 tttcgaaaaa gtattctaat aacaaaatta gagaatcttc gcacacatat caaaacattg
    11281 aaacgatttg cttatgggtt tcgcagtttt accaatatgc gcttgtggat ttttctaatc
    11341 aacaggttaa tcagctatgc attaaaagct agtaactctt actaactttg ttgttcataa
    11401 atgaccttat cagcccgatt tgacaaagaa ctactttttt acaacattat tttattttcg
    11461 agtaatcttt cttatttcat ttcgtatatc tggaaaaaga aaataaacaa acggaagata
    11521 aataatgaaa atcattattg atttaattgc catatctaaa aatgaagaag agaataattg
    11581 tggaagaaag taataggccc aagtagtcaa agtaagattg actataaaaa ataagataac
    11641 gttcttccca aagaaatagg atatttttat ttcttttcgt acaaaaaata ctctaaagaa
    11701 aaagactaat aattctacta ccacgagtac gatcgtagcc gctacagcgc cataagtagg
    11761 cacaaaagct acatttccaa taatacttac aatcgcacca atgatcattg gataagcata
    11821 ctccttatct ttttctatag caattgcaaa ttggttagca aaaactccac caattggact
    11881 aacaactata attagacaag aaataagcat gtttattgtc atcggtttga aattctcccc
    11941 aaaaaaccat ggaacaaatt cttttgtatt gataaacacc acactaaaaa gaattaatgc
    12001 aatgataatc gtgtaatcaa aagatttttt tgtgtaaact aaaatatttt ctgatttccc
    12061 ctctgcctgt gctttagata ttttaggcaa cataacaata gtaattgatg ttaagactgc
    12121 caatataata cgagcaattt tttgagactg atcataataa gataattccg ttggatccac
    12181 aaaataccca actatcgttt tgtctaaagt tgtgtacact tgcataaaca actgaggagt
    12241 caaaataata aaagctggtt tccacaactg tttaaaaaca ttagagttaa agattggttt
    12301 acctactttt ccgtggatac ttaaccagaa aactaaactt cctaaaaagt tagtaattga
    12361 agtaatgaac acgtaaatcc acaaatcatc tttagttgaa acaaaaatga ttattaacaa
    12421 taccgatgat aatttgataa ctgtatttct aataaccact ttcgaaaatt cttcaattcc
    12481 ttgaaaaaac caagaaatat ctaatgttag ccctacaata aatatcaatt gaattaagta
    12541 taaattttta tactttacat caaaaaaata aatataggct atatagagaa aggaaacact
    12601 caagcctatt aaaaattgaa taaagtaaat attccagaat tgtaaagata gtgctttttt
    12661 atctttcctt gatttagcga tatttcgaat acgaaaagga ttcattccca cagcaatgac
    12721 agtcattaaa aactgtgata ttgagaaaac atacccataa atccctaaac cagttgaacc
    12781 aattctacga ctaagtatag gtatagtaag taagggtaaa acaatcaaaa agacttgata
    12841 tatcgcatta taaaatatat cttttactgt ttttttcact ttatatccct ctctatcaac
    12901 tcaagaacct tttctgtact tgctcctgtt tcataatttc caatataatc aagtaactct
    12961 tgagaacttt tcaaaagctt ttcttgttgg tttttaaaaa catcaacaag gccataacta
    13021 tcttgtacgc ttataattgg taactcactt aactgtatat aaaaacctct ttctaagcta
    13081 tattcttttt catcatagtt aaaaagaata attggttttt ttgttagcgc aaaatcgaaa
    13141 aaaatagatg aataatctgt gattaaaaca tctatctctt caaaaagcat ttcaagagga
    13201 atcccatcat taattaaagt aatttgatgt tttttcatat attctatact tttagaatca
    13261 gttgctaatt tactatgaac attaggatga aatttagtca taaaatttaa ttcttgttgt
    13321 tgctttttaa aaaattcacg taagttatcc acacctaacc attctagttg cgtaattccg
    13381 ttgtcctcaa tattatttcg aaaagttgga gcaaataaga cattgatttg tttctttttc
    13441 aagtcaaaca cttgctcttt actagaggct aattcatcgt ttctaggagt tcctacttct
    13501 aatattttag aaggaacgat attgaatgct gaaacatact tcttagaact atagcgatta
    13561 ccagataata aatatgtaat tgactcacta tcttttttcg catagcttag gtaacgttta
    13621 ggtaacttat cttttgcatc aaattcaatt ttttttaaag gcgttccgtg ccatgtttga
    13681 aaataaagtt gttttttcct ttttgcatac ttaatcgttt tccgactatc gtctatccaa
    13741 tacttagctg tatataaata aaaggcagct aaaaaagaat cggagtcaat tcttttaata
    13801 taatctggta aatcagttat aatcgcttct tttttgatta accaaataat tctgtaattc
    13861 aatttttttt catgaatttt ctct
//
LOCUS       DACEMS010000070        13874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEMS010000070 DACEMS010000000
VERSION     DACEMS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.071"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(6..662)
                     /locus_tag="IW735_001813"
     CDS             complement(6..662)
                     /locus_tag="IW735_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1044809.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            855..1133
                     /locus_tag="IW735_001814"
     CDS             855..1133
                     /locus_tag="IW735_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044810.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1152..2360
                     /locus_tag="IW735_001815"
     CDS             1152..2360
                     /locus_tag="IW735_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1044811.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2508..2873)
                     /locus_tag="IW735_001816"
     CDS             complement(2508..2873)
                     /locus_tag="IW735_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1044812.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3020..3793)
                     /locus_tag="IW735_001817"
     CDS             complement(3020..3793)
                     /locus_tag="IW735_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1044813.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3967..4827)
                     /locus_tag="IW735_001818"
     CDS             complement(3967..4827)
                     /locus_tag="IW735_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1044814.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4827..6245)
                     /locus_tag="IW735_001819"
     CDS             complement(4827..6245)
                     /locus_tag="IW735_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1044815.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6302..6751)
                     /locus_tag="IW735_001820"
     CDS             complement(6302..6751)
                     /locus_tag="IW735_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1044816.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7196..7459)
                     /locus_tag="IW735_001821"
     CDS             complement(7196..7459)
                     /locus_tag="IW735_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1044817.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7558..8853)
                     /locus_tag="IW735_001822"
     CDS             complement(7558..8853)
                     /locus_tag="IW735_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1044818.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8850..10121)
                     /locus_tag="IW735_001823"
     CDS             complement(8850..10121)
                     /locus_tag="IW735_001823"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1044819.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10286..11155)
                     /locus_tag="IW735_001824"
     CDS             complement(10286..11155)
                     /locus_tag="IW735_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1044820.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11447..13057)
                     /locus_tag="IW735_001825"
     CDS             complement(11447..13057)
                     /locus_tag="IW735_001825"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1044821.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITSEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3948 a         2922 c         2369 g         4635 t
ORIGIN      
        1 gttcatcaaa agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg
       61 tctttaacgg tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta
      121 gcagttgaga tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc
      181 atcccttctt ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca
      241 gctcccagct ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga
      301 tagcggtaat actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa
      361 aagcgcacac gattgattcg ataaaactct gcatattctg attcaactcg aatattatac
      421 ggcgaatcga tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg
      481 atatagcgaa ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc
      541 tctttcaaga tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat
      601 cctttgcgta taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc
      661 aatgatgctc ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt
      721 taaagtccaa aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac
      781 ttatgcgatt tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa
      841 tagagggaaa aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat
      901 atcaaacaag atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact
      961 cgatcgaatt gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa
     1021 atctgtttat gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca
     1081 attatcacga tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat
     1141 gtataacata catggaaaag aaattattta ataaatattt tattggaatc acattgatca
     1201 attttattgt ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag
     1261 acaccttaca cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa
     1321 cattgctggc acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct
     1381 taagaggcgg cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta
     1441 tcggggcatt gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag
     1501 ctacaaatgc tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt
     1561 attatggatt aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc
     1621 tgaatatgac tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta
     1681 cgattgcctg ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat
     1741 ctcttcgctc atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa
     1801 ttgccttttt gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa
     1861 aagtaatcca cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca
     1921 catttacacg tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt
     1981 atcctagcta tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg
     2041 tgacattgct tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg
     2101 gacaggctgt ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct
     2161 actttattgg tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat
     2221 ctcttacttc atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag
     2281 cactctatct tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat
     2341 cattgaaaaa tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa
     2401 taatctgagc ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt
     2461 tatttttaaa gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc
     2521 cgttcatctg gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca
     2581 ccgatcaata gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa
     2641 tatagataga gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg
     2701 ttccctactt tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt
     2761 accccaacaa tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac
     2821 agaatcgccc agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc
     2881 actcctttct cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat
     2941 accatcggct gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt
     3001 agaacattca cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg
     3061 catactgttc ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa
     3121 tggcttgatg catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt
     3181 ctgattgttt tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc
     3241 cttggctgaa atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg
     3301 catcgttata ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca
     3361 atacagatac ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg
     3421 taatgatctg caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac
     3481 tggaatccaa gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct
     3541 tacgtttttc tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt
     3601 ttcgattgtc aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca
     3661 gaaaaacaag gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg
     3721 tgactcttgc aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca
     3781 cttcattcaa cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt
     3841 gacagaagtg attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac
     3901 atcgtgtgtg gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt
     3961 ttatttttag tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac
     4021 cacttcttgt acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg
     4081 attttcactc aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa
     4141 attcactttg gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga
     4201 accgccatgt aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat
     4261 atttaatttt ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc
     4321 aagtgtatcg attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat
     4381 gattgtttca gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc
     4441 cactgagaca tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc
     4501 gtcgaatgat ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg
     4561 taagacatct tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt
     4621 ataagcatag gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg
     4681 atgaatttct aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat
     4741 catatcatag ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt
     4801 caataactct ttcattgtat ataacattac gcttccacct catttgttag attttcattt
     4861 ttcgtccact tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag
     4921 gctgtagtga ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca
     4981 tttggaatta gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc
     5041 atggaaatcc ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg
     5101 tcagataccg caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat
     5161 gattgacctg aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa
     5221 gcaataccta ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact
     5281 ccagctgaca tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata
     5341 tcagctgcca tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag
     5401 ctattcagcc aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat
     5461 accatcaaaa gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata
     5521 gcagaaaatt ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct
     5581 agcacgccac caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg
     5641 gcaacaattc ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct
     5701 aacgcaggaa ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct
     5761 aacacattat agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt
     5821 gcgatgatga ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa
     5881 tgattataga ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg
     5941 aatacgccaa tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg
     6001 cttgtagaga cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct
     6061 actgcaagaa tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt
     6121 tttacacctt gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat
     6181 ttttctgccg acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt
     6241 ttcatgatta ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt
     6301 ttcattcttg actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct
     6361 ttctaaaatc tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat
     6421 ctgctgattt ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc
     6481 cgctatagtc cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt
     6541 tgctttttgc atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc
     6601 gtttgaatag tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca
     6661 catcccctat tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca
     6721 gttcttctgc gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta
     6781 acgttttctt ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa
     6841 tgaacagagg aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc
     6901 tcacaaacaa tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc
     6961 ttctaatttt aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat
     7021 aaggtgtttt tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca
     7081 ttatgatcct acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa
     7141 aaacagacta tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt
     7201 ccgcagcgtt tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg
     7261 cttgtgtgaa tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac
     7321 ggatgactgg gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg
     7381 aacctgacaa gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg
     7441 gatggatgtt ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag
     7501 agttgatttg tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta
     7561 tcttttcaga tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa
     7621 aaattgctga ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa
     7681 tgaatctcca gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat
     7741 caataattct tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg
     7801 ttcagataat tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata
     7861 gatcacgtca tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact
     7921 gccaccttct tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc
     7981 tgggtcaatc ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg
     8041 agcaagtcta gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg
     8101 cattgcgcgt tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa
     8161 tgtagaagat accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg
     8221 ttcatcaatc agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc
     8281 gatttctttt aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg
     8341 accaaaaccg attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt
     8401 ttccagttga agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc
     8461 ttttgcttct tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg
     8521 ttccgattct ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa
     8581 tcgtctgatt tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg
     8641 ccgtaaaaaa ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa
     8701 tccttgtttc tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt
     8761 actgtaataa ggaattttaa attctttggc ataactataa acttctttta acgttttgtt
     8821 ctctagttct gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca
     8881 attgatgccc ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa
     8941 tctacattgt aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca
     9001 cctgcccgaa gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg
     9061 atgatcatat ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg
     9121 ttcacacgtt tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt
     9181 aacgcagtaa gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg
     9241 tttgcgggtt ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata
     9301 tggacaccaa tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt
     9361 ttcactttga taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg
     9421 cggtcaggaa tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc
     9481 acatctgtac ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct
     9541 atgatttccg gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca
     9601 gctaacatca cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca
     9661 cgcaatcctt tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca
     9721 cctagagctt caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca
     9781 cctggcaggc ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa
     9841 gaagcacgca aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga
     9901 tcgatttcta gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca
     9961 attaatgaat gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct
    10021 aagattgcag ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca
    10081 ccagctaaag gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat
    10141 taatcttttt tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg
    10201 gtctgccgtt ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta
    10261 tatatcgtat ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat
    10321 gagatttagc atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga
    10381 atcctttacc ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg
    10441 ataattggaa ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga
    10501 tttgatcttg aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag
    10561 catcagcaat tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac
    10621 catggatgtt accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc
    10681 attcttcaat atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg
    10741 aaccaacttc acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag
    10801 ctaatttcaa gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc
    10861 ctaattcgat acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg
    10921 gaacagtgat gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac
    10981 cacccatgta tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt
    11041 cagccgcttc aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag
    11101 cgtaaccgcc tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa
    11161 aattcctcct aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta
    11221 ttttaccaaa catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa
    11281 gttttttgca aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc
    11341 tatctttttt cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc
    11401 ttatcccctt ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt
    11461 gatctgctaa tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag
    11521 aaatcaattc tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga
    11581 tttctactaa acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca
    11641 attgacgata tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg
    11701 ctccatcata tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa
    11761 ggcgtaaagt tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat
    11821 tgtttgctgt atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac
    11881 gtgcaaattc gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat
    11941 tttcgcgagc ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac
    12001 caggaaccaa gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt
    12061 cttgtgcttt gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca
    12121 atgcttcaac tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa
    12181 tccgtacttt tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt
    12241 ctgcttctgg tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt
    12301 gtaaaactaa tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg
    12361 gttcgacatc acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg
    12421 tacgtaccac caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct
    12481 gcgttggttt agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga
    12541 tatacataac attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg
    12601 gcaaagattc tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag
    12661 tcattttagc tgctcgcatg attttttctt tgatctcact tgtaatatga ggaataactt
    12721 gaacagttgc tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt
    12781 ttcctgtcgt cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc
    12841 ccagatccaa atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg
    12901 ggctcatcgt ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta
    12961 agccgcggtt tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg
    13021 aaacgacacc gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat
    13081 ttatgtgcag gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg
    13141 gagccgaaat ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa
    13201 gctgactaag ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt
    13261 tcgatatcct ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc
    13321 acataataag cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt
    13381 actgaaaaat tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa
    13441 tatcccacct aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc
    13501 gtagaaacaa tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca
    13561 cagttctcgg atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc
    13621 actaattttt tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa
    13681 ggtaacgaat cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt
    13741 ctaaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca
    13801 tgagaagctg tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc
    13861 tctggataaa cggt
//
LOCUS       DACEMS010000071        13741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEMS010000071 DACEMS010000000
VERSION     DACEMS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.072"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(221..1177)
                     /locus_tag="IW735_001826"
     CDS             complement(221..1177)
                     /locus_tag="IW735_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1044822.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IW735_001827"
     CDS             1281..1883
                     /locus_tag="IW735_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1044823.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IW735_001828"
     CDS             complement(1971..3470)
                     /locus_tag="IW735_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1044824.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IW735_001829"
     CDS             complement(3486..4010)
                     /locus_tag="IW735_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044825.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IW735_001830"
     CDS             4308..5057
                     /locus_tag="IW735_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1044826.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IW735_001831"
     CDS             5048..5833
                     /locus_tag="IW735_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1044827.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IW735_001832"
     CDS             5802..6518
                     /locus_tag="IW735_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1044828.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IW735_001833"
     CDS             complement(6620..7639)
                     /locus_tag="IW735_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1044829.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IW735_001834"
     CDS             complement(7658..8716)
                     /locus_tag="IW735_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1044830.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IW735_001835"
     CDS             complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IW735_001835"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1044831.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10161..10997)
                     /locus_tag="IW735_001836"
     CDS             complement(10161..10997)
                     /locus_tag="IW735_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1044832.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11181..12398)
                     /locus_tag="IW735_001837"
     CDS             complement(11181..12398)
                     /locus_tag="IW735_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1044833.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12781..12864
                     /locus_tag="IW735_001838"
     tRNA            12781..12864
                     /locus_tag="IW735_001838"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12815..12817,aa:Leu,seq:gag)
     gene            complement(13104..13727)
                     /locus_tag="IW735_001839"
     CDS             complement(13104..13727)
                     /locus_tag="IW735_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044834.1"
                     /translation="MKFTNIKKYRAFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILT
                     ALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIA
                     TLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYI
                     LYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4150 a         2803 c         2373 g         4415 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
     8881 aaaaaacctt aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat
     8941 gcgtggaaac gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat
     9001 acgataaagt ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag
     9061 ctgattgctg gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag
     9121 tctacaacat ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat
     9181 tctagacgtg ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca
     9241 tcaggcatcc caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct
     9301 ttttccatta ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca
     9361 gaaaagacat gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat
     9421 ccacgcatac cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc
     9481 gcttcttcta gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact
     9541 tgtttgacaa actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa
     9601 ccacctgaag cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt
     9661 gggttttgtg cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg
     9721 attttagctc ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa
     9781 cgttctttgc ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga
     9841 ccttcagaca tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag
     9901 ccgtgaatat gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg
     9961 ttgacttctc cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca
    10021 agatagcctg catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt
    10081 ctcattctat tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag
    10141 tcaagaatgg tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag
    10201 ccttcataga atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc
    10261 acaaataaat cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga
    10321 ttcacctgtt ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta
    10381 taaaaaattt gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg
    10441 atgcttcgac tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt
    10501 ccgatacaac taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta
    10561 gagtagctta atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg
    10621 aattcgcaaa caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt
    10681 tcagaaatat cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa
    10741 caactatcca acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc
    10801 atcacatgat caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc
    10861 ggcagttcct cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa
    10921 agcatttttt gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac
    10981 tcgattaacg tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat
    11041 tttactttag gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt
    11101 tatttaagaa aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact
    11161 gaaaaatttg gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc
    11221 ttagctgttt gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc
    11281 tggttccaat taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc
    11341 aaaacgaatt tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata
    11401 taaaaagcag cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag
    11461 atatattctc tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga
    11521 taaccgccta gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg
    11581 gaggacaaca tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg
    11641 accatccagc ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta
    11701 acaacgaaca aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact
    11761 aagcgatcat agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg
    11821 atttcttcta ataattcttt tggataaagt gccccagtag gattattagg attaatgata
    11881 acaatagctt ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat
    11941 tctgcctctt catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc
    12001 gtccacaacg gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc
    12061 tgcatacaca tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc
    12121 acattcggaa aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc
    12181 cccttggaat ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact
    12241 tcgtttggtg cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta
    12301 atgccctctt cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg
    12361 acaccttcta atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc
    12421 aaaaagttag aactttccaa attttttgtt atttactatt tcaatatcat actccatcct
    12481 agaaaatttt gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt
    12541 gagaacttaa ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc
    12601 acaaatgatt cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac
    12661 acctaagatc attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc
    12721 tcccattttt gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac
    12781 gcagatgtgt tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg
    12841 tgggttcgag tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt
    12901 ttttgtttga acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga
    12961 acgtacaatc atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt
    13021 agaaacttgc aagtgttaaa acaattaatc gaaaaacgag taaaatccct cacctaatac
    13081 taaagcgagg gattttttgt atattatctg tttatcaccg ttattataaa ggtacaaata
    13141 ccaagcacgg ttaatccaaa ttgcataaat tcattttttt gtacactcat gcttttcatt
    13201 agctctttaa ctgccattag tactaagatg gcgataacaa cataaagtat atagtacgtc
    13261 cgcattccgg aagcccatac tgtgggagaa aatcccataa ttaaccttga acaaaatccc
    13321 attatcatca aaataactaa aaaaatagaa gttttcttat tgtcaaaact taaataaatt
    13381 ccaacaatta aacatagtaa taatgccaag actactaaag tagctatcca agtacctgga
    13441 taaaaaagtg acagtttcgt tcctaactct gtaaagagat tattcaggtt actactactc
    13501 caaatcattg cccttttatt tccattgacg taagtaaagc tttgacccat tgtattacca
    13561 aaataaataa tcaggtttaa gaaaaacggt aatgcagtca atatacgcac ataatagttt
    13621 tgacatttcc tatatgttag aaggaaaatt aagaaaaata gcaataaaaa taagatattc
    13681 atgtctaaaa aaaaggctct atactttttg atgttggtga acttcaccaa catctttttt
    13741 g
//
LOCUS       DACEMS010000072        13536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEMS010000072 DACEMS010000000
VERSION     DACEMS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.073"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(60..1007)
                     /locus_tag="IW735_001840"
     CDS             complement(60..1007)
                     /locus_tag="IW735_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1044835.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1197..1466
                     /gene="rpsN"
                     /locus_tag="IW735_001841"
     CDS             1197..1466
                     /gene="rpsN"
                     /locus_tag="IW735_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1044836.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1642..2004)
                     /locus_tag="IW735_001842"
     CDS             complement(1642..2004)
                     /locus_tag="IW735_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1044837.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2015..3136)
                     /locus_tag="IW735_001843"
     CDS             complement(2015..3136)
                     /locus_tag="IW735_001843"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1044838.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3152..3502)
                     /locus_tag="IW735_001844"
     CDS             complement(3152..3502)
                     /locus_tag="IW735_001844"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1044839.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3632..5143)
                     /locus_tag="IW735_001845"
     CDS             complement(3632..5143)
                     /locus_tag="IW735_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1044840.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5465..6817)
                     /locus_tag="IW735_001846"
     CDS             complement(5465..6817)
                     /locus_tag="IW735_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1044841.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6970..8046)
                     /locus_tag="IW735_001847"
     CDS             complement(6970..8046)
                     /locus_tag="IW735_001847"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1044842.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8242..9987
                     /locus_tag="IW735_001848"
     CDS             8242..9987
                     /locus_tag="IW735_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1044843.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10236..10703)
                     /locus_tag="IW735_001849"
     CDS             complement(10236..10703)
                     /locus_tag="IW735_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1044844.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10687..12060)
                     /locus_tag="IW735_001850"
     CDS             complement(10687..12060)
                     /locus_tag="IW735_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044845.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12381..12869)
                     /locus_tag="IW735_001851"
     CDS             complement(12381..12869)
                     /locus_tag="IW735_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1044846.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12891..13463)
                     /locus_tag="IW735_001852"
     CDS             complement(12891..13463)
                     /locus_tag="IW735_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1044847.1"
                     /translation="MSSDHAHYWSVFFFVLILYAYHHGIFRSSTSLQAFIQQFGKYAV
                     MIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAIGFLLVRYYGV
                     AFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVAGLTKLSFREF
                     MQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3862 a         2938 c         2458 g         4278 t
ORIGIN      
        1 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
       61 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
      121 gcttggatga catgcttaag ggaagcagac agattcattg aaataaaaac atgaaaggct
      181 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
      241 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
      301 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
      361 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
      421 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
      481 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
      541 ccgaccgcac ccgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
      601 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
      661 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
      721 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
      781 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
      841 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
      901 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
      961 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
     1021 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
     1081 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
     1141 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
     1201 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
     1261 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgcaaa cttcctttag
     1321 atgctcgtcc agaacgattg aaaaatcgtg atttgatcga tggacgccct cgcggataca
     1381 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
     1441 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
     1501 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
     1561 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
     1621 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
     1681 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
     1741 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
     1801 tattcctgtt ccagtagctt tgatcccaat atgagtcgga agcttaggtt tagccatttt
     1861 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
     1921 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
     1981 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
     2041 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
     2101 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
     2161 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
     2221 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
     2281 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
     2341 ttccttcacc tgctgggagt tttttgactt gaattaattc agaaaccagc tcaagtgctg
     2401 gctgcagtgg ataaacttcc ggtagtgcgt gcccagaggg attcagtccg tacattgcta
     2461 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
     2521 tgacatgtat atatcgagga agttcgggaa gtttttttag cacttctata aagcgttctt
     2581 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
     2641 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
     2701 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
     2761 tctctagctt acttttctct aaaacagcag ctgctgcctg caaccattcc cgtgtacctg
     2821 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
     2881 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
     2941 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
     3001 gaccgtatcc gtttgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
     3061 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
     3121 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
     3181 ctgtttcatt tgtatgagta atactgaccc atactttgcc ttcatgaggt gaacgcgtca
     3241 caacaggctg accgttttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
     3301 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
     3361 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
     3421 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
     3481 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
     3541 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
     3601 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
     3661 cgcgtttttt atcgttgtga cgtttaccgc catctttttg gctgttatta tttttgctat
     3721 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
     3781 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
     3841 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
     3901 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtacttat
     3961 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
     4021 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
     4081 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
     4141 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
     4201 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
     4261 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
     4321 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
     4381 attcgtctac acggcgtttt gtacgtccga agacgattgt tagttcaggt gtttgaacat
     4441 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
     4501 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
     4561 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
     4621 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
     4681 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
     4741 tgatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
     4801 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
     4861 cgcgccctaa acgatataat tcttcttgtg tttggatagc taactcacgt gttggtgcga
     4921 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
     4981 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
     5041 ctagtggaat cgtttctgat tggataggtg ttgcttcttc aaaaccagcg cgttcgactg
     5101 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
     5161 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
     5221 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
     5281 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
     5341 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
     5401 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
     5461 atttttattt catttgctga agctgttcta ccacttcaaa taagcccatg ctgttgctgc
     5521 cttttagcat aatactatct gttgcttcaa gattttgttt tacagcatca atcaatttat
     5581 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
     5641 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
     5701 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
     5761 acacagcgat tctgcgtcct tttgttggta gatttttaaa agtatctaat accagtccca
     5821 tggctgttgg atttgcatta tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
     5881 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
     5941 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
     6001 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
     6061 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
     6121 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
     6181 cgtctggtac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
     6241 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
     6301 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaag acgatctttt
     6361 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
     6421 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
     6481 tggttttacc attgcttcct gtaattgcaa cgacttctgc acctactttt tccaagtaat
     6541 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatctt
     6601 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
     6661 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
     6721 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
     6781 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
     6841 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
     6901 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcttcttctt
     6961 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
     7021 taattgaatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
     7081 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
     7141 gtttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
     7201 agctaacttc gcgtactctt gcgctttttg ataaacttct tccggcactt cggctggaat
     7261 ctgcatttcg attttattat tgatatattt ggcttcataa tcatagaatg ctacgtcttt
     7321 tacgacttcg ccaggcaaag tcgtccgaac atcttcattt cctaatacag caacttcgat
     7381 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
     7441 taaagcattt tgcagctctt ctcggttttc tgcctttgta atgccgacac tagaacccat
     7501 attcgcaggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
     7561 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
     7621 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
     7681 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
     7741 cccgtttggc ccgtgtaaaa ctggaaaaac gatggctcct tcttctttga tttctcccgg
     7801 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
     7861 caagacatct ttgctagcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
     7921 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
     7981 aaatgcggaa agaatggaaa cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
     8041 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
     8101 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagttggttt ctaaaagaac
     8161 aaacgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
     8221 taaagaaaag gagggatgat gatggcgcaa acaaatccac gtagccgctt ggcagaaatc
     8281 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
     8341 caggtaaaaa aagcctttga agaactaggc cccaccttca ttaagatagg acagatgctt
     8401 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
     8461 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
     8521 tcattatcag atatattcga tgatttttcc aaatcgcctt ttgcctctgc ttctatgggg
     8581 caggctcatc gcgccaagct gaagaacggt gactcggtcg tcgttaagat ccaacaccct
     8641 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
     8701 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
     8761 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
     8821 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
     8881 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga accatttaat gaacgccgaa
     8941 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
     9001 aagcttttag ttgagaactt tatgaaacag gtatttgatg atggtttttt ccatgctgat
     9061 cctcatccag ggaatctgct attccatgta ctaaaaaaag aagaacagac acaagctagc
     9121 cgaaaaactg aaatagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
     9181 tctacagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
     9241 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
     9301 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
     9361 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
     9421 gatgaaatca acttgcaaga agtattcaca cacgtcgtga cgatttgtca tcaaaataat
     9481 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
     9541 atccgcgtcc ttgaccctga agtttccctc atggaagtcg cttcgccttt tgcccaacat
     9601 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
     9661 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
     9721 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
     9781 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
     9841 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
     9901 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
     9961 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    10021 aaataagaag tgattttgca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    10081 ttcgaagagg ctacttctga agcaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    10141 cagtaataag aacaatcaaa aaggaacatg gagcacattt atcctgtttc cctgttccct
    10201 ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc tgtacttggt
    10261 aaaccttggt caattgcctt ttttaacgct tgacaaaaag acatatgatc tttctccgta
    10321 aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact atgataacgt
    10381 tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg atggaacact
    10441 cggacgccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat caaaaagtca
    10501 ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg gtctacgtag
    10561 acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact ggtcacgata
    10621 accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac tggagaacca
    10681 tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat aaacagttac
    10741 taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca gttgtacact
    10801 ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg gctctcccat
    10861 ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca cactcccttt
    10921 attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg catcagtttg
    10981 ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa gcagaaagac
    11041 acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca gtccaagcaa
    11101 ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga ctatttttgc
    11161 tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt ctaatccgta
    11221 cattatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca ttccatttag
    11281 aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt gtcctaaaac
    11341 aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa aaagcctttc
    11401 atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata tcaaaaataa
    11461 aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca gttcgtaacg
    11521 cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct
    11581 tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa atagccagat
    11641 agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct tttcgtgaaa
    11701 attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca aaacacttga
    11761 aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac cagcaccacc
    11821 aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta gactgttgac
    11881 taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca
    11941 attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa ccgtgttact
    12001 taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat
    12061 gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag cattaaaggg
    12121 gaaataaacc aattttacag aatatattct tgtaaaaaaa atacagaaag aagcataagc
    12181 ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag
    12241 tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc
    12301 tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta
    12361 ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga
    12421 aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa
    12481 acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt
    12541 tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact
    12601 ttatttagca aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg
    12661 taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct
    12721 ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca
    12781 atgatcggca caaaagcttc cggccatact tcttgtatga ctgccatcag ctcatccagt
    12841 tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga
    12901 ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac cgaccacggt
    12961 ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact
    13021 agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta
    13081 atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa
    13141 ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca
    13201 atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc
    13261 gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga
    13321 acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc
    13381 gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa aacagaccaa
    13441 taatgggcat gatctgaaga catttttgta aaactgatcc ttttttagac atctatattc
    13501 cccacatctt ttttcattta ttaaaactgc ctcctt
//
LOCUS       DACEMS010000073        13415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEMS010000073 DACEMS010000000
VERSION     DACEMS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.074"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            233..475
                     /locus_tag="IW735_001853"
     CDS             233..475
                     /locus_tag="IW735_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ1044848.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            472..942
                     /locus_tag="IW735_001854"
     CDS             472..942
                     /locus_tag="IW735_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1044849.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1070..2284
                     /locus_tag="IW735_001855"
     CDS             1070..2284
                     /locus_tag="IW735_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1044850.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2994..3182)
                     /locus_tag="IW735_001856"
     CDS             complement(2994..3182)
                     /locus_tag="IW735_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044851.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3273..3482
                     /locus_tag="IW735_001857"
     CDS             3273..3482
                     /locus_tag="IW735_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1044852.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3594..5207
                     /locus_tag="IW735_001858"
     CDS             3594..5207
                     /locus_tag="IW735_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1044853.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5242..6453
                     /locus_tag="IW735_001859"
                     /pseudo
     CDS             5242..6453
                     /locus_tag="IW735_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6476..7402
                     /gene="glsA"
                     /locus_tag="IW735_001860"
     CDS             6476..7402
                     /gene="glsA"
                     /locus_tag="IW735_001860"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1044854.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7610..9070
                     /locus_tag="IW735_001861"
     CDS             7610..9070
                     /locus_tag="IW735_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044855.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9291..11054
                     /locus_tag="IW735_001862"
     CDS             9291..11054
                     /locus_tag="IW735_001862"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1044856.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11138..11596
                     /locus_tag="IW735_001863"
     CDS             11138..11596
                     /locus_tag="IW735_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1044857.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11705..12388
                     /locus_tag="IW735_001864"
     CDS             11705..12388
                     /locus_tag="IW735_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1044858.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12535..13149
                     /locus_tag="IW735_001865"
     CDS             12535..13149
                     /locus_tag="IW735_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1044859.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13165..13371
                     /locus_tag="IW735_001866"
     CDS             13165..13371
                     /locus_tag="IW735_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044860.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         4671 a         2117 c         2802 g         3825 t
ORIGIN      
        1 agaaattgat tgtcgttttc ttttgcagaa atttaagtgc aggcaattat ggtagttgtc
       61 gtaattgctg tcagctcagt gtttatcact gatggtgtga aagatttacg gttggagatg
      121 ttcaataaga ttattggtgc ttaattattt ttattgtagt gctgatatga gaatgttata
      181 attggggtat aaaacataca aataaatttg ctgaaaaatg aggtatcaaa atatggaaaa
      241 tgaattactg gaaaacttag gaagacttca cacaactgaa cttggcgtta ttagaattaa
      301 aaagaactta tctttgaatg tagaaaatgt aattgaatgg tgcaaagaaa aaattagcct
      361 ttctaatgcg gaaatcacaa gaaaaggaaa aaactggtat ataacgattg ataattgtat
      421 tataacggta aatgcgtaca gttataccgt tatcactgct cacaaggtaa aatgataaat
      481 ttttctataa aggaattaaa cgcatttgca gttattgggc aggaagtaga actgactaat
      541 tatcaaaaaa agaatattca gattagtaca cagttttgga gaaagtttaa cagtagtttg
      601 aaaaaagcgt atctttcaca atcaggaaat tgggtaaaat atgcttttat ggaaagaaga
      661 aatggaaaac tttattattt ctgttctatt ccccaaagaa ccattacccc agagggcttt
      721 ctgtataaag aaataccgtc ttataaatat ttggttgtgg agcatattgg tgctatggat
      781 aaaatatatg aaacttatgg caagatttat gaagaaatta ttcctagtac atcgtatatt
      841 cctataaaag atattatcct acactttgaa aaatacgatt atcgttttca ttggaatagt
      901 gataattcag ttattgaaat ttggatacct attcaagatt agaagcaatt ttatatccat
      961 acacacaaag cagttagtct taggattgac tgctttttct atacagattt ttaatgacag
     1021 gcaattatca attatggtag ttgccttttt tgattggagg aattgaagaa tgaatgataa
     1081 aaactttatt gaagaactaa gacaaaagcg tgaggaatac ggagtaacac aaacaaggct
     1141 tgctgttgcc tgtggtatca gccgtgaata ttacaaccgc attgaaaaag gaaaacagcc
     1201 attgaatgac gaattaagag aaatcataga aaaacagatt gagcgtttca atccgcaaga
     1261 accactattc ttattgattg attactttcg tgtccgcttt cctactacgg acgcattggc
     1321 gattatccgt gatgtattac aactgaaatc taattacatg ctttatgaag attatggaaa
     1381 atacggatat gaaagcaaat atgtacttgg cgacatcaat atcatgtgtt ccatgcagga
     1441 gcatttaggt gttttattgg aactgaaagg caaaggctgt cggcaaatgg aatgttatct
     1501 gctggcacag gaacgctcat ggtatgactt catgctggat tgtatgacgg ctggtggtgt
     1561 gatgaaacgg cttgatttgg ctatcaatga taaagcagga atattggata ttccaaagtt
     1621 aaaggaaaaa tacaaggctg gcgaatgtat gtcctacttc cgtatgcaga aagattacag
     1681 cggtacagaa aaatgcggta gcgatttacc aaagaataca ggagaaacct tatatctcgg
     1741 ttcaacaagt agcgaattat atatgtgtgc ttatcagaaa aattatgagc agtatgtcaa
     1801 gaatggcaca gaaattgaag atacggagat taagaaccgt tttgaaatac gcatgaagaa
     1861 cgaacgagcc tattatgcgg ttgtagattt actgacctat cgggacgctg aacgcaccgc
     1921 tttttctatc atcaatcatt atgtccgctt tgttgacaga gaggacgaca agccaaaaag
     1981 tcaatggata acaaatgatg attgggcatg gtttgtaggg gaaaacagag agccgatacg
     2041 cttaaccaca aagccagagc cttacacttt acaaaaagcg ttgcattggc tacaaagaca
     2101 agtcgcacca accataaaaa tggtacaggc attagacaga gaaaatcgta caacgatact
     2161 gaaagatatg attgagcagg cagaactaaa agacaagcat aaacatttat tacaattaga
     2221 gaaatcaacc atagaagaac gtatagatac cgctgttcca caagagaatg acggtatttt
     2281 ttaatgtaat tgaaaaattt cagatttagt cagcaaagag ctgttttcat tccctatgtg
     2341 aagtgaaaga agaaaaataa attttttatt aaatctgcaa caaaatggct acttgcgtac
     2401 agatatggtg cgagaaaagg aaatctaaac tttttttgaa aataggtcat taaatcgggg
     2461 cttgctgtcc ctatatgaag tgaaagaaga aaaaagtttt gaaaaaatga cggaaattcg
     2521 tgattagtgg acagtcttct gtgaaaagag gaaaaacatc atcattttgt attcaaacag
     2581 gcattagcag ttgagtgttg atgtgaaaag atgaaaatac tttgttctca acttaacaat
     2641 ttaaggtttc cgttccctat atggtgcaaa gaaaatattt cattctattg ttggcagtaa
     2701 cggtatggtt tgggtagtta gggtgtgaaa aggaaaatat tcttttttca tcatcaactt
     2761 agcaaaatga cattttctat ccctatatgg tgcatgaaaa gaagttaaaa tttttccatt
     2821 taaacttagc aaatggacgt ttgctgtaca gatagtagtg aaaaaggaga aaatcaaccg
     2881 ttgattgcca aaatgaaccg ttccatttgt actaataatg gaaaaagttt tggattttgg
     2941 ttacgtttct cctcttttcc aacgcgatat ataggaaaga cgattttttt ctttcaatcg
     3001 ttctttgaca actgaataaa gctattcaat acgtttgaca gacagctagc cgttgaaaca
     3061 aatacgccac gacctttccc ctgcaagagc gagcgaaaac ctatttgtgt tcttgtaaaa
     3121 gatttgtcct cttttatcgc catgacctgt actgtcagat aatgatactc ccgtacagcc
     3181 acagcttgag cattaagagc gtcgcacgca atgggtacgg ctacataaga actatgcaga
     3241 ggtggaactc ctgtgggcta tgacaaaagc cgttgaattg cttagaaaga ttttacagaa
     3301 agggggtatg tgttattgat aatgctgtaa aaacaattca aaagaaaaat ggcaagtgtg
     3361 gcattggcaa cagaggaaag acaaagattg tagtaaaaga gcatttttcc gaaaagggca
     3421 aaacaatgga agaacttcta actgatgtca tgctggaaaa agcaaagcag acaatcgcat
     3481 aaaatcggtg cagttgtaag aaatagattg aatgacaaaa agtacccgtg ttatacttta
     3541 cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg acaatgaaac
     3601 aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa ttacagggcg
     3661 aaagttccag cattaccaca cagagaagta tgttgcgttt atatgcaaaa gaacatcatt
     3721 tgaatgtcat tgatgaatat attgatgacg gctggtcggg aacaaatttt gacagaccga
     3781 gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt gtaacgaaag
     3841 acctttcccg tcttggcaga aattatatta tgacaggaca atatacagaa ttgtatttcc
     3901 ctagtcataa tgtccgctac atagcgattg atgacggtgt agacagcgaa aaaggcgaaa
     3961 gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat acaagccgta
     4021 aagtcaaatc agcatttaag acaaagtttg cggagggggc tcattacggt gcttatgctc
     4081 cgttaggata taagaaacac cctgacatca aaggaaaact gttggttgat gatgaaacaa
     4141 aatggattat tgaaaaaatc ttttccctag cctatcaagg ttacggaagt gcaaaaatca
     4201 caaagcagtt aagagcagaa aaagttccga cagcgtcatg gctaaatttt acaagatacg
     4261 gtacttttgc acatatcttt gagggaaaac ccgaaagtaa gcgttatgag tggacgattg
     4321 ctcatgtcaa ggcgatattg aaaagtgagg tttatatcgg aaacagtgtt cacaatatgc
     4381 agtctacggt atcgttcaag agcaaaaaga aagtgcgtaa gcccgaaagt gaatggtttc
     4441 gagtagaaaa cactcacgag ccgattattg acaaggaagt gttctatcgt gtgcaggagc
     4501 agataaaatc aagacgcaga cagacaaagg aaaaggcaac gccgattttt gcagggcttg
     4561 tcaagtgtgc ggattgtggt tggtctatga gatttggaac gaataagaca aataaaacgc
     4621 cttacagtta ttatgcttgc agttactacg ggcagtttgg caaaggtaat tgttctatgc
     4681 actacattcg ttatgatgtg ctgtatcaag ccgtattgga acgcttgcag tattgggcta
     4741 aggcagtaca gcaggacgaa gaaaaggtat tgaacaagat acagaaagct ggcaatgcag
     4801 agagaatacg ggaaaagaag aaaaaggcaa gtacattgaa gaaagccgag aacagacaaa
     4861 atgagattga ccgtttattt gcgaaaatgt atgaagatag agcctgtgag aagataacag
     4921 agcgaaattt tgtgatgttg tccagcaagt accaaaaaga acagatagaa ctggaacagc
     4981 agataacaag cctaagagaa gaactaagta aaatggaaca ggatatgata ggtgctgaaa
     5041 agtggattga gttaatcaag gaatattccg taccaaagga actgacagca ccattattaa
     5101 atgccatgat agaaaaaatc ctcatacatg aagcaacaac gaatgaggat aacgaaagaa
     5161 tacaggaaat tgagatatat taccgattta ttggaagagt tgagtaatca acaagagtaa
     5221 tatttttaac taagggaaac cggcggtgtc tatacttggg tcagtcgcac attgggaaaa
     5281 cgattcggtt ttgccgcaat ctttttccaa tggtttgaga taacagttgg ctacttgacc
     5341 atgctgtatt tcttaactgg tgcattatcc tatgcaacgg gtattggtgc tatacaaaac
     5401 aataaatatc tgaaattggc aatcctattg attattttct gggctatttt actctcacag
     5461 ttaaaaggaa ccaaatacac ttcattgatt gctcggatcg gttttgtagc tggtatcttg
     5521 ttgccagcat tagtcttgtt tggcttaggg atccattatg tcgcttcagg cgctccacta
     5581 caaacgatac tgtctatgaa aacattgatt cctgattttt cgaaattgcc tacgctggtt
     5641 gtttttgttt cctttatttt agcatatatg ggcgtagaaa catctgccag tcatgcaaat
     5701 gaaatggaaa accctaaaaa ggattatcca ttagcaatgt ttgtattggt tattctggca
     5761 atcattttag atacttttgg cggattgacc gtcgctacta cagtacctca acatggtttg
     5821 tctcttaata caggggtgat tcaatcgtta gaattcctat taagacacat tgatccaaat
     5881 cttgcatgga ttgcgaaaat tcttgcgatt cttgtttgtt taggtgtcat cggagaaatt
     5941 gcttcttggg taacctctcc ttcaaaagca cttcacgtag cagcagaaga aggattactt
     6001 cctgaatatt ttgcaaaaga aaacacacac ggtgttcctg ctcatttgat gatcgcaaat
     6061 gggatcgttg caacaatctg ggcagccgtc tttacattaa gcggcggggg gaataatctt
     6121 tcatttcttg cagcgatgtc attgactgtt gtaatctacc tgatgttgta ctttatgttt
     6181 ttcgcgggct atttgaagct ggtttttaaa gatgacagtc ttgaaagagc ctatcaggtt
     6241 ccaggcggaa aagttgtaaa agtaatcgtt gcttgtttag gtcttttgac ttcacttttt
     6301 gctttttgta tctcatttgt tccacctgct agtatcgcag ctggacagca tgggatttat
     6361 gaaacgattc tgattgtttc ttatcttatc gtcttagtta ttccatttac gatctacgca
     6421 tgccgtcata aattccagca tgcagaaaaa taaaaaatga aagctggtta ttcaaatgga
     6481 agtaaaagaa ttagaacaat tgattggaaa aaatttccac tattatcaaa caggacaagt
     6541 ggcggattac attccagcat tagctgaagt ggatcccaaa caattaggta tggcgattta
     6601 tgatttggat acaaaacaac tgattgaggg aggagacgcc gatgtgcgct ttgccatcga
     6661 aagcatgtca aaagttcccg tattgttatt agcaatcaaa gacaacggaa tagaaaaagt
     6721 ctttcaaaca atcaacacgg aaccaaccgg ttttgccttc aattctatca tgaatatgga
     6781 aatcaatcat cgcaaacatc caatgaatcc ttttgttaat gcaggagcga ttgcaacaac
     6841 ctcgttgatt gccggtaaag atgcagatga aaaatttgag cgaatcttgt catttatgaa
     6901 agagatctgt gatgatccta atatcacatt gaatgaagaa atctatcatt ctgaatcaag
     6961 aacaggtgat atcaatcgct ctcttgctta ctatatgaaa ggtaatcaaa tgatcgaagg
     7021 agacgtaccg gagatactcg atgcttattt cagacaatgc tccctcaatg tcaccgctac
     7081 aggcatagca aaattagctg ctgtgctggc aaataaagga attgctcctt ggaatggcaa
     7141 acaaatcatt actgaagaaa gtgctacaat cgtcaaatca attatgacaa ctgctggttt
     7201 gtatgatgaa tcaggagagt tttccgtcca tgttggtgta cctgcaaaaa gcggtgtagg
     7261 cggcgggttg atggcagctg tgccaaatcg ttacggcata ggtgtcttca gcccggcact
     7321 agatccattt ggtaacagcg cagctggtat ccaattgttg aaagatgtag tcaaagaact
     7381 agacgctgac atctttgaat aaccattctt gaaagaggct ggggtaaaag tccaaacggc
     7441 tcaaagctac ctacaatgag gctgaaattg tgagcttgtt tgacttttgt cccagtttct
     7501 ttttttattg gtttttgaaa gggcttttct aaataaaaag gaaacaaaaa tacgatttac
     7561 agatatgatt aaataaaatc ataagaaaaa ctggaggcgt gtatcaggaa tgctggaaaa
     7621 ttacatcgag cgaaatatat ttagaaaagt gtatctgtgt gaacaattat ttgaattcca
     7681 agaaatagac atcgaacaaa cagctatttc tctaagggtg actacaccaa cgatccttca
     7741 cgatctagaa agtctagccg agtgtctaga atattgtata aaagaacaag taagagagaa
     7801 gcataagtat aaactcgtat tcaaacatgg tattgctctt tctgagctca cacagtttct
     7861 gtacggtcaa tcttattttt taaagttttt aagttattat tttaatggta tattcacgag
     7921 tacagaactg gctgatcttg aatttatttc tttgagtaaa gtatatacaa tcaaaaaaat
     7981 cgtattggat ttttttaaag aaaatgatta tttgaaaaat aaagaaatca ttatcccaga
     8041 atttgactcg cggaacatct tgctagcttt agtacgttat gtcaattggg aaggctatga
     8101 aaataaaaat ccgtatgtag aagaagcttg taacgagttg attgattatg tagagactca
     8161 tttttttaaa agaagatatt cggaagatga aaagtccttt atcatacgag aaatagagat
     8221 cgcaatcgga agaaaggcat ttccccttgg actgaaagaa gaagataaga aaagcgcaga
     8281 aacgaagccg ttatttcaat tagtctgtca aggattggaa gagcaaaaag gaattctaga
     8341 cttacaagaa gaagatgtct attatatttt ttctttgttc aacagtagaa attataccaa
     8401 tgagaacatg gagctgttaa gaaaagattt cgaagttgtt tataaaaatt ttgttctcaa
     8461 tgacctatca ctgaatgaat tgattttaca gattattagt atcgctgaaa tcgacgatgc
     8521 agaaaatttt attttcaagc aatccttttt acaatttgtc cgtacgttgt gggcagatgg
     8581 acaagtattt ttaccggaaa ggatctatct tctatcagaa aaagaacaac ttctgtacgg
     8641 aaaaattgta gaaatattgg aagagtggaa aaagaaaaat caaatccaac taagatggaa
     8701 tgaaaatttc attcgaaaat tcgtcaaaag tctttctttg atcaacagtg aacaagcggg
     8761 gagacaggaa atagaaattt ttgttgtttc agaaagttca gtcaaacaat ttttttatcg
     8821 aacccaattt catttgtatg cggaagaagg catcgctgaa ataaatccta ttttatttcg
     8881 ttcattagaa gaattgcctg atgaatgtct atgtgcgaaa aaaagagtgg ttttgtgcga
     8941 tgctgctagc tatcaaaatg gattagaaac tgaaaagaca aagatctatc caatctcatt
     9001 aaaaggtgca aacatccatt tgtatcatat tgctcaggaa cttcacctat taaaaaaaca
     9061 tatttcttga tgagacctta attaggagac cttctacaaa gctttttaat tgtctgtaga
     9121 aggtttttat ttgagtcctg attcgtgcga agatctactt atataacaag ctttctctga
     9181 aattttttta aaatgctatt gacttagagt caactctaag tagtagaata gataaagtgt
     9241 aacaaatcta ttttcattaa aagaagatag tacggaggag agaatgtatt atgagtacag
     9301 tattacatgt attggatgca atcaatagcc ccgcagatgt caaaaaatta tctgttgagg
     9361 aaatgaaaca gttggcagaa gagatgcgcc aaaaaatctt gcaaaaggac agcgatgtag
     9421 gtgggcacgt tggtccgaat ctaggtgtag tagaattaac gattgcgttt cattatgtat
     9481 ttgattcacc aaaggataaa atcgtttggg atgtttccca tcagtcttat ggtcataagc
     9541 ttttaactgg tcgaaaaaat ggttttgaaa aaggacatta ccgtgaagtg agcggatatt
     9601 ccaaccaaca tgaaagtaaa catgactatt ttacagtggg gcacacatct acatctatca
     9661 gcttagcaac aggtttagct aaagcgagag atctgaagaa agaatcagga aatgtggtag
     9721 catttatcgg tgacggctcc ctaagtggtg gcttagcttt tgaaggattg aacaatggtg
     9781 gtgttttaca atccaatcta atcgtattag taaacgataa tgagatgtct attgatgaga
     9841 atcatggcag tttatataaa aacttagctg agcttcgtca aaccaacggt aaaagcagca
     9901 ataacctatt taaagcaatg ggatttgatt atcgttacgt ggataatgga aacgatctgg
     9961 aaacggtgat ccgtttattt gaagaagtaa aagatattga tcatccgatt gtacttcacg
    10021 tccatacgga aaaaggtcgc ggttatcagc cggcaatcga taataaaatg aaatatcatt
    10081 ggcatgtacc atttgatctg tccaccgggg aggcaaagca agtaacttct ggagaaacat
    10141 ttaatatgat catcatggat tatttggata agaaaatgca aacaagtaca gctcctattg
    10201 ccgcagtcaa tgcagcgata ccaggcgcct ttggactgaa aacattccaa gaaaaacatc
    10261 cagatcatta ttttgacgtg gggatcgctg aacagcactc tatcactttc gcttctgctt
    10321 tggcttctca gggagtacgt ccagtcgtct tccaatcaag tactttcttg caacgggcat
    10381 atgatcaatt atcacatgat ttagcgatca acgagaatcc agtgattatt ttagtcggtg
    10441 gcggaacgat ctcagctagt gaccgaacac accaaggaac atttgacatt ccttacttat
    10501 caagtatccc gaacatcaaa tatcttgctc cagcaacaaa agaggatatg atctctatgc
    10561 tagattgggc gttcgaacaa acagcgcagc ctgtagcaat ccgaattcct acacatggtg
    10621 tagaacatgg tacagctatc gcaaatgatt atagtattcc agcctatcaa acagtaaata
    10681 atggaggaaa agtagcaatt ttagctttag gcggttttct tgaattagga aaagaaacag
    10741 taaaagcttt tagaaaaaaa ggaatcgaag caacgttgat caatccagga acgatcacag
    10801 aaaatgacgt agaaacatta gataaattaa aagaaacaca cgaattagtc atcacattag
    10861 aagatggtag tttgtcagga ggatttggcg aaaatatcag tcgttattat ggtgattctt
    10921 caatgaacgt attaaatttt ggtgcagaga aagaattcac ggatagcgtt cctgtagggc
    10981 agctttatga acgttatcat ttaacaccaa aacaaatcgt tgaagatagt ctgcgtgtct
    11041 tgaatattca ataattcatt atgctcacca tccagataat tgtttatact agtatctgga
    11101 tggttttttc attataaaaa gcaaaggggt ggatgttatg tatcagcaag tagaagaata
    11161 tgtcaaaaag ttccaagaaa aatatcctag ttatcaatca gaaaaattag gtttcttttc
    11221 atttggggat acagaaaaaa tggcagacga gttgagcgaa ctagttgtaa aaaagataaa
    11281 gactggtaca tcaagtggtt acgatctgta taaacaagag aatgagcctt taccaaaagt
    11341 tggtcaatta gacgtggtcc tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat
    11401 aaatgtgacc atcttgcctt ttagtgaagt cacgaaagag catgcttgga aagaaggaga
    11461 aggggatcgg acattagctt attggcggaa agtccatgct gagtttttta aacatgctta
    11521 taaagaagcg ttgaacatag aatttacaga agagaagctg gtcgtatacg aagaatttga
    11581 agtaattttc ttttagtaag aaaggtctgc tgaaagaaat agctcattat tgtctcctga
    11641 atagaaaagg gttacatttt tatatgaagc tgtattttta tataacatta ctggaggaga
    11701 tattgtggat acactggaac aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga
    11761 aaacgagccg ctacacagtt cttttattat ggggagaatg gctttggctg caaaagaagg
    11821 cggagcagtt ggtattcgag caaatacgac agaagatatc aatgaaatca agcgaatcgt
    11881 tgatttaccg gtaataggga ttatcaaaag agattacgat gggtcagaag tgtttattac
    11941 tgcgacagaa aaagaagtgg aagaactttt ggagacgcaa gcagaaatca ttgcactgga
    12001 tgcaacttct cgtaaacgtc cgacagaaaa aacaactgct gaacttgtcc aaatgattca
    12061 cgaacacggt cggttagcta tggcagatat ttcgactttt gaggaagcgc ttcaagcgca
    12121 agaagatggt tttgatcttt tgtctacgac tttagcaggc tatacagaat attcacgaaa
    12181 gacgcagaca cctgattttg atttattgaa tcaaatcata gaacaagtcg atatcccagt
    12241 aattatggaa ggacatactg actctcctga acaagtagag aaagcatatg aattaggtgc
    12301 tttttcagtc gtagttggat ctattatcac ccgtccgcaa ttgatcacta aaagatatgt
    12361 cgaagccgca agtaaagcaa ataactaaaa aacacgcacg gcacagattc ttgacaaaaa
    12421 agaaaaattg atagatagtt taaagcagat tctaagaaga tgaaaaaagc attacttttc
    12481 attttgtctt caagatctgc tttttaacga attttataga aagaaggcga aaccatgttt
    12541 gaacggagtc cttcagaatt gacagaacgt gaaaattaca agttgctgat tggttcagtc
    12601 atccctagac cagtagcagt agtaacgact aaatcagcaa aagatatagt gaatatcgca
    12661 cccttcagtt acttcaatat cgtcagttcg aatcgaccca tcttatcctt ggcgatccag
    12721 agaaaacaag atgcagtaaa agacacagca aagaatattc ttgagacaag agaagctgtt
    12781 atccatatcc ttgatgagga caacgtagaa gaagtaaata aaacagcagc caatctgcct
    12841 aatgaggaaa gcgaactgta cctgactgac ttgactttaa ctgattcagt aatcatttca
    12901 gtaccagcat tgacagaagc aaaagttcgt tttgaaacat ccttatatga acatgtcgag
    12961 atacggaata aaaataaagt aacagctgat ttatttttgc tagaagtaca gaaatatcat
    13021 ttaaatgaaa cagtctatga tgataagaca ggaaggatcg atccaagaaa attgaatgct
    13081 gtaagccgtt tggcaggaaa tgattatgca gcaataggag aaattttcac gattgcacgt
    13141 cccgaataaa aaggagcact agttatgtta aaaataagct ataaaccatc gacagattca
    13201 aaagaaatga aaaaagaata tgaaacagtg aatgattttc tgcaagggca gtacttagaa
    13261 gttccgccat tgcaagacca ttttgtcgtt actactgtta ctttagatgg taaggaaata
    13321 gaaatgccag atcaaacgat tagcggattg tttaattact ttaacaaata aatatttatt
    13381 ccgaaaagtg aaaaaacata taaaaaaaca cataa
//
LOCUS       DACEMS010000074        13212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEMS010000074 DACEMS010000000
VERSION     DACEMS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.075"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1016
                     /locus_tag="IW735_001867"
     CDS             147..1016
                     /locus_tag="IW735_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1044861.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IW735_001868"
     CDS             1225..2250
                     /locus_tag="IW735_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1044862.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IW735_001869"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IW735_001869"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1044863.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="IW735_001870"
     CDS             3333..5036
                     /locus_tag="IW735_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1044864.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IW735_001871"
     CDS             5038..7071
                     /locus_tag="IW735_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1044865.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IW735_001872"
     CDS             7082..7522
                     /locus_tag="IW735_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1044866.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IW735_001873"
     CDS             complement(7722..8849)
                     /locus_tag="IW735_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044867.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IW735_001874"
     CDS             complement(8852..9529)
                     /locus_tag="IW735_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044868.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IW735_001875"
     CDS             9793..11559
                     /locus_tag="IW735_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1044869.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IW735_001876"
     CDS             11574..12011
                     /locus_tag="IW735_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1044870.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IW735_001877"
     CDS             12024..13175
                     /locus_tag="IW735_001877"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1044871.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DACEMS010000075        13185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEMS010000075 DACEMS010000000
VERSION     DACEMS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.076"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(31..588)
                     /gene="frr"
                     /locus_tag="IW735_001878"
     CDS             complement(31..588)
                     /gene="frr"
                     /locus_tag="IW735_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1044872.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(591..1313)
                     /locus_tag="IW735_001879"
     CDS             complement(591..1313)
                     /locus_tag="IW735_001879"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1044873.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1449..2330)
                     /locus_tag="IW735_001880"
     CDS             complement(1449..2330)
                     /locus_tag="IW735_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1044874.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2429..3211)
                     /gene="rpsB"
                     /locus_tag="IW735_001881"
     CDS             complement(2429..3211)
                     /gene="rpsB"
                     /locus_tag="IW735_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1044875.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3570..4049
                     /locus_tag="IW735_001882"
     CDS             3570..4049
                     /locus_tag="IW735_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1044876.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4266..5357
                     /gene="phnW"
                     /locus_tag="IW735_001883"
     CDS             4266..5357
                     /gene="phnW"
                     /locus_tag="IW735_001883"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1044877.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5350..5478
                     /locus_tag="IW735_001884"
     CDS             5350..5478
                     /locus_tag="IW735_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1044878.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5482..6027
                     /locus_tag="IW735_001885"
     CDS             5482..6027
                     /locus_tag="IW735_001885"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1044879.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6480..8171)
                     /locus_tag="IW735_001886"
     CDS             complement(6480..8171)
                     /locus_tag="IW735_001886"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1044880.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8591..9538)
                     /gene="arcC"
                     /locus_tag="IW735_001887"
     CDS             complement(8591..9538)
                     /gene="arcC"
                     /locus_tag="IW735_001887"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1044881.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9653..10672)
                     /gene="argF"
                     /locus_tag="IW735_001888"
     CDS             complement(9653..10672)
                     /gene="argF"
                     /locus_tag="IW735_001888"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1044882.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10763..11992)
                     /gene="arcA"
                     /locus_tag="IW735_001889"
     CDS             complement(10763..11992)
                     /gene="arcA"
                     /locus_tag="IW735_001889"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1044883.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12453..13154
                     /locus_tag="IW735_001890"
     CDS             12453..13154
                     /locus_tag="IW735_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1044884.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3692 a         2696 c         2353 g         4444 t
ORIGIN      
        1 atgatccgcc cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc
       61 agcagcgatc ttatctactt ctttgatgct gttatctgtc aatgtttgta cgtctttttc
      121 taatccgcgc aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc
      181 gatcgcatca cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac
      241 atctttagct aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac
      301 atttccatca ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc
      361 ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat
      421 tgatgccatt tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa
      481 aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc
      541 tgcttttttc attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc
      601 cttacagttg ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt
      661 aaattgaaga caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc
      721 atgacttgta atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca
      781 tttgcatcta ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg
      841 ataacatctg cgccgatttc tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac
      901 gggttgccgg taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct
      961 ttacggcgaa tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc
     1021 ggtacaccca cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac
     1081 atacccatgt aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc
     1141 cagatatttc cgccgcccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg
     1201 atttcttgga tgatttcttt gattactggt ggtttgattc caaatcctgc gtccccagct
     1261 aaagcttctc cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt
     1321 cctccaatat tttctattgc cattttacca tacttagatg atttcgacac tctaagactg
     1381 gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt
     1441 caacagaatt attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt
     1501 tcgatacctt cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca
     1561 acgtattttt caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt
     1621 tctgctttga atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg
     1681 ccttcgttca atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga
     1741 atttgtgatt cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt
     1801 gcaactgttt cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata
     1861 tgcaagtatc caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag
     1921 ctgatttttt ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta
     1981 tctgttttga ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct
     2041 gcaatttctt ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag
     2101 tttacttcaa cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct
     2161 gcaatgcggt catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact
     2221 gctttttcca tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg
     2281 ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc
     2341 tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca
     2401 gcctttgaaa cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa
     2461 catctacgat ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta
     2521 cttgatcttc accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt
     2581 taacggcacg aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc
     2641 agtttgtatc aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa
     2701 tacgttcttt acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga
     2761 taccgcctaa gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt
     2821 taggtagaac ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga
     2881 tacgtttttg gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga
     2941 agtattgtcc tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac
     3001 ctacgaataa tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat
     3061 ctactaattt aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga
     3121 tatatttctt catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt
     3181 ctagtaattg tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt
     3241 tcctcttctt atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat
     3301 aagaatgtgg atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg
     3361 tatcgcaaga gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa
     3421 agaattcaag aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat
     3481 aattttaggt ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca
     3541 tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta
     3601 aacaagtcat tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc
     3661 attatggcgt taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg
     3721 tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc
     3781 ctgagactag gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga
     3841 aagtcgaacg agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat
     3901 ttgcatctgt tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg
     3961 atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc
     4021 accatccaac tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt
     4081 gtttttttat tttcaatatt ttccttattg aataaacctt tacactcttt acaaaaatac
     4141 atcatttttt attcaaattt ttcaacacct tgattgaatc acaataatca tcatttatat
     4201 tggatgttaa caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa
     4261 atcatatgta taaattatta acttctggac cattgacgac ttctgaaaca gtgaaaaatg
     4321 ctatgctgat cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa
     4381 gaaaaaaatt attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc
     4441 aaggtagcag ctccttcggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata
     4501 ctgtactgat ttgttcaaac ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc
     4561 tgaaactgaa ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga
     4621 ttacacagct gattgaagag aatccgtcta tctccgcact agcaatggtc catagcgaga
     4681 ctacttccgg tattttgaat aatttggaag agattgctgc tgtcacacaa gaaagagatc
     4741 ttttgtttat agtggacgcg atgtcaagct ttggtgggat accgatttgt gtagccgaac
     4801 tagggattga ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt
     4861 cttttgtact tgctaataaa gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt
     4921 cacttgatct atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca
     4981 cttcccctac ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag
     5041 gaggaatcga aactcgatat tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa
     5101 tgacagagat ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt
     5161 ttctttaccc taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa
     5221 atggatttgt gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata
     5281 ttggcgagat ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg
     5341 agaacgttaa tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc
     5401 tgtatggctc ctgtccacgc ttttagaaat gcttttctag aaaaaggaac ccaactgact
     5461 gacaaagaaa tccgctaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt
     5521 tccctccctt ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa
     5581 gaggtggagg aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat
     5641 tctaccttga aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat
     5701 attggttcca cgaatggatt taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa
     5761 aaagaaaggt attcaccaga cttcatggca acttttgaag acgtcgaagg gtatggtcga
     5821 ccgtttcctt atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa
     5881 gtaatcaaag tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg
     5941 acagtaggtg taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa
     6001 ttagagatgg ggaaaaagat cgtttaaaac agtctgttgc tcttagattc caacaagccg
     6061 gcgctgatat ttgtgtaaac aatttaaaag agttaacaga atacattttg tacgctgaca
     6121 agaatccaga atgactttta tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag
     6181 taataaaaac agccaagcaa aaatagctcc tcatattttt cgtttggtta ggcttattac
     6241 cgcagatgca agcttttgaa caccgtttat tcgaaaaaaa aagggagctg tgacacgact
     6301 tttgtcacag tccccttatt tgcaaacaaa cagtgtttaa gagacaacct ctccactaaa
     6361 cggtgttcat tcagctgact ctcggtattt gctcaaaaaa cgataaaatt tgaaaagcta
     6421 ttttctcttt tgtttctcta atactcggat cagaacgctg atttcacatt ctctttttct
     6481 tacatcttat ctggtgcatg aagtcctagc aaacgaaggt cttctttcaa taatacagtc
     6541 actgcataaa ctagagctaa gcgtgcttct ttttgttcat catcagctaa aatcttcgta
     6601 tgagcatagt atttgttgaa tgcttgtgct aatttgattg catgtttcgc aatcactgac
     6661 ggttcgtatt tttccccagc agacaagacc gtttcagggt atttttgaac taatttaacc
     6721 acttcccagc tggtatcgtc attcaatgca tagtctgctg tttctgaagg gacaaatcct
     6781 gctttttcca acaggctgac tgcacgagca tgtgtatatt gcacataagg tccagtttct
     6841 ccttcaaatc gaacaacttc ttccaggtta aagtcaaatg tattcagtcg atcgtttttc
     6901 aaatcgtgga aaatgactgc cccaacccca acttgtttag ctacagcatc tttgttttcc
     6961 aaatcagggt ttttctcgct gatttgttct ttagcagatt ggattgcttc atttaatact
     7021 tcttctaata agacgatctt tcctttacga gttgataatt ttttaccacc ttgcgtgatc
     7081 aaaccaaatg gaatatggtg catgtcgtca gaccagtcaa agcccatttc cttcaagaca
     7141 gctttcaact gtttaaaatg atagctttgt tcattcccta ctacatatag agattgtttg
     7201 aagtcgtaag tacgtttacg gtacaatgca gcagctaagt cacgtgtaat gtatagtgtc
     7261 gcaccgtctg acttcttaat caacgctgga ttcaggtcat aagcagatag gtcaacgatc
     7321 tctgcacctt tatcctcgtt cagcaaatgt ttttcttcta aaagtttgac gatttcatcc
     7381 atcttgtcat tgtagaaagc ttcaccgttc aacgaatcga aacgtacttc cagcagatca
     7441 tagattttat tgaattcttt catcgattca tcacggaacc attgccataa ttgaatagcc
     7501 tctttatcgc cttcttccag acgtttgaac caagcacgag cttcttcatt taattctggt
     7561 tctgtttcag ctacttcatg gaattggacg tataaacgaa gcaattcatt gattggttct
     7621 gctttaacag cttcttctgt accccatttt ttgtaagcga cgatcagttt tccaaactgt
     7681 gttccccagt cacctaaatg gttgatgcgg attggctgat aaccgatttt ttccataatg
     7741 aaaccgatcg aattaccgat aactgtcgaa cgcaaatgtc ccattgagat tggtttggca
     7801 atattaggag aagacatgtc gattggtact gttccttgat tcccgatgtt gctatcccca
     7861 taatgttctt tttctttcac tactgtttgc aaaacttttt tgctgataag ttctttattc
     7921 atgaaaaagt tcaagtaagg tccgacaact tcgatttttt caaagttggc agaatcgatt
     7981 ttttctgcta aatctgcagc gatttgctgt ggcgcttttc ggtaaacttt tgctaatgaa
     8041 aaggctggaa atgcaacatc tccgtgttca gctgattttg gattttctag taattgctcg
     8101 acttgttcca gtgttaaatc atctttgaca gcgtcataga cagcttttgc gacaagatct
     8161 ttattattca tctttattcc tcctaaagtg ttcccttgat tgttaaacaa aaaaaatctc
     8221 tagcgacggc atacttagta tgccatcact agagacgaga tttcccgcgg taccactcca
     8281 attgtcctaa aaaaggacca gctccatctg atatcggtag acgaccgtct gtttaaaaca
     8341 gaattctcca agtgcgcttc gttgtatctt cttcattcgg cttccaccac ttccgaattc
     8401 gctagccaag aatgatacaa gtactctctt gttcaacaaa tcttagtctt attaaacttt
     8461 cttgaattat atcagaaaaa aaaacgaaag gctagtcgtt tcataaaaaa aaaatccaga
     8521 tggcctaaag gagaagaaat accacctgga tagctataaa tcatttcgta aatacgaaag
     8581 acttaacaaa ttaatcagct acgataatcg ttccttcttc agaagctaaa agattctcga
     8641 tattttctaa agaagtgatg attgccttag cgtttggttt tgcttcaaca aatgcaatag
     8701 ctgcttctac tttcggaagc atgcttcctg gagcgaattg cttttcatca atgtattgtt
     8761 tcatttctga aacagtaaca gtttctaatt tcttttgatc tggtttttgg taattgacat
     8821 acacattgtc tactcctgtc aatacgatca gcaaatccgc atcgatgatt tcggctaatt
     8881 tttctgaagc aaaatcttta tcgattacgg cttctcgtcc ttctaatcca gtagctgttt
     8941 ctacaacagg aatcccaccg ccaccaacag agactgtgat aactccttga tcgaccagcg
     9001 tttcgatgac acgtgcttct ttaatcgaga tgggtttagg ggaagcgact acttttctcc
     9061 atccacgtcc agcatcttct ttgaatgtca cagtggagtc agcattcatt tgttctttgg
     9121 cttcttcttc cgtgtagaat ggaccgactg gtttacttgg atttttgaat gaaggatcat
     9181 tttcatccac aataacttga gtgactaaag aaaccacatc tttatcgatc cctttttcct
     9241 tcagtacttc gcccatggcg ttttgtaacc agtaaccgat agatccttct gtcattgcaa
     9301 cacatgtatc taaaggcatt gccggtgttt tctcagaatc agcagcttgc tgctgaatca
     9361 ataggttacc gacttgcgga ccatttccgt gggagatgat caattcatct ccttgttcga
     9421 taaatttcac taaatacttt gctgtttcca ttaacgcttc ttgttgggct tttgcacttg
     9481 catccgtaga caagatggca ttgcccccta aagcgacaac tacttttcgt ttggccattt
     9541 cattcgtcct ttcttcagtt ctaccgaatt ttctagataa caaaacgagc cagcgttttg
     9601 tgattttccc tcaatgctga ctcgtttttt cgtgagatga atgtggttat gcttaaacgc
     9661 gagggataaa caagttacct aatgttgctg ccatgatcgc tttgattgag tgcatacgat
     9721 tttctgcttg gtcaaattga cgagcatatt tgctgcggaa gacttcatct gttacttcca
     9781 tttcagtgat gccaaagcgt tctttcattt gttcaccgta aacagtgttt gtatcatgga
     9841 aagctggcaa gcagtgcaag aagatcaaac gatctgtgtt gtgtgttttt tctaccattt
     9901 ccatattgat ttggtaaggt ttcaacaatt tgatacgttc ttcgaattta tcttcttcac
     9961 ccattgatac ccatacatca gaataaagga cgtctgctcc gtctacacct ttatcaacat
    10021 cgtcggtaat catcaattgt gcaccggatt tttcagcaaa gccttctgcc attttgacga
    10081 tttcttcttc aggttgtaat tcttttggtg caacgatacg catattcgct cctaaaattg
    10141 cacctgtgac taataaagag ttagccatat tgtttcgacc atctccgcag taagcaacag
    10201 tgataccttc tactgttcca aagttttctt ggatagttaa aaagtcagcg atcatttgtg
    10261 ttggatgcca ttcatcagtc aagccattcc aaactggaac accagagaat tctgctagtt
    10321 cctctaccat tttttgactg aatccacgga attcgattcc gtcaaacata cgtcctagta
    10381 ctttagctgt atcttctgtg gattcttttt tacctaattg gatatcattt gcacctagat
    10441 attcaggatg tgcacctaga tcgatcgctg cagtagtaaa tgcagaacga gtacgtgtag
    10501 atgttttttc aaataaaagc gcaatatttt ttccttctag atagtgatgt gggataccac
    10561 gttttttcaa gtctttcaag tgttctgaaa aatcgatcaa atattgtaat tcttcttttg
    10621 taaagtcttt ttctgctaat aagcttcttc cttggaatac tgattctttc atattaaaca
    10681 ttctccttta gattgttttt atttaagatt aaagaggaaa tcccgacggc agctgacagt
    10741 aagactgccg tcaggaaaca aattacttca agtcttcacg gacaagtggt tggctcatgc
    10801 agcgtggacc accacgacct cgtgacaatt cacttgaatt gatttctaac actttgatgc
    10861 catagctacg aagcaattcg ttcgatacat aatttcggtt ataagtgact acaacacctg
    10921 gagcgattgc caatgtgttt gatccatcat tccattgttc tcgaggtgca acgatctcat
    10981 ctccattacc agttgggatc agtacaagat catctaatcc aagagcagct ttcatcgttt
    11041 cgtgtaagtt atctgaatga gtaatcttga tatcatcacc atctggttcg attgtaaaga
    11101 catccatctt accgtcttta ctttgaattg ctggatggat agtgaattta tcgtgatcta
    11161 ccattgtaaa tacggtatcc aaatgcatca ttgcacggtt gttcggaatt ttgattgcca
    11221 acactttttc aaatcctgaa ttttttgcga ataggtggcg tgctaatttt tctaaagctt
    11281 tcgcattagt tcgttgagaa atcccaacag ctactacttt atcgcttaag atcaattcgt
    11341 ctccgccttc gatatgatct ggattttcac gatccaacca tacttcgatt cctttatttg
    11401 caaatcttgg atgatgtttc aaaatatatt ctgtaaacat cgattcacga cgacgtgctt
    11461 caaaagtcat cttattgact gtcatcccat ttcccattga tgcggaaggg tcgcgtgtaa
    11521 agtacaagtt tggcattggg tccatgtaaa atggatagtc atcgtctgct gacagatcat
    11581 atagactgct agaacgtact tcgatatctt ttgtacgaac cccagccatg attttatcta
    11641 ccatttcttg cgtttccatt gatagaagga actcatgaag accctctctt acagcattcg
    11701 atgcaatatg agattcatcc aacattttat tcaagaattg ttctttgaca ttgcctgcgt
    11761 caatcgcttc tgccgctaat ttttccaagt aaagagtctc gacgccttca ttttgcaatg
    11821 tcttcgcaaa gttgtcatgt tcttcttgag caatcggaag atatggaata tcatcgaaca
    11881 acagacgatc cataatatct ggtgtcaaat tttccacttc ttgtccaggc cgtttcagta
    11941 aaactgtctt taatttccct atttcagaga aaacgtgaat aggtttatcc atgttgtgtt
    12001 gcctccttct ttttgattac atgtatagga tatcctcctt ttgtaatcac tttcataatt
    12061 ttgtctcatt caaaatgaga cattcctcac attaatattc gataattaaa taattaaacg
    12121 tttttctatt tttaagcgta atttattcat tttttattct gtatacactt tcaaaaaaat
    12181 atttattcat tgaaaaaatt tttttgaata aattaaaaaa ataaattata ttatcgttgt
    12241 tagcgtttac tatatgtatt attgttgttt tttatacaca aatcattcgc ttcaaaacaa
    12301 attattcaat accattgtat attttttaca taataacaaa ttatatttgt aaagtattaa
    12361 aacttgtttc taatgttggt atgttaaaac tttacaaatt ctttaaaaaa agtgacaata
    12421 aatatagtga acaataagga gaggactttt taatgcgtgt taataaaatc gattttcact
    12481 tttcaaaact cagatatcaa cctgattttg aacatttctc ggatgaagag ttccaacaga
    12541 tcaaggaaca tactgttctt cgttcttata aaaaaggaca gatccttttc gacgatggtg
    12601 atcccagaca acggttgtat atcttgacca aaggactcgt tcgtttagaa cgttatgatg
    12661 aaagtgcaac ttattactac tatgactatg tgaatacaaa taaccttttt cctatgtcag
    12721 gaatgtttgg agaagagacc tacacttata cagctcaagc gatgactgat attgaaacat
    12781 tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct
    12841 atatgataca aaaaatttct aaagtagttg aaatacatga attgcgtatc caaatcggat
    12901 tgacctctag cgcctttgac cgggtaaaac aaaatttatt cattttgaaa gaagaattgg
    12961 gcgtaccaac aaaagatggt catatcacca ttccttatcc gatcacctta aaagaattag
    13021 cagtgaatag cggcacgaca cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt
    13081 ccgggctgtt agattatgac aaaaagcgtt ttactttctg taagacagct actactccag
    13141 ctatgagaga ataacgcgaa caaaaataag cagtgaccct gataa
//
LOCUS       DACEMS010000076        13049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEMS010000076 DACEMS010000000
VERSION     DACEMS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.077"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW735_001891"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW735_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1044885.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="IW735_001892"
     CDS             1873..4539
                     /locus_tag="IW735_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1044886.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="IW735_001893"
     CDS             4903..5655
                     /locus_tag="IW735_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1044887.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="IW735_001894"
     CDS             complement(5677..6831)
                     /locus_tag="IW735_001894"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1044888.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="IW735_001895"
     CDS             7016..9457
                     /locus_tag="IW735_001895"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1044889.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9787..10491)
                     /locus_tag="IW735_001896"
     CDS             complement(9787..10491)
                     /locus_tag="IW735_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044890.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10678..10923)
                     /locus_tag="IW735_001897"
     CDS             complement(10678..10923)
                     /locus_tag="IW735_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044891.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10940..11791)
                     /locus_tag="IW735_001898"
     CDS             complement(10940..11791)
                     /locus_tag="IW735_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1044892.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11957..12961
                     /locus_tag="IW735_001899"
     CDS             11957..12961
                     /locus_tag="IW735_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1044893.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4169 a         2315 c         2716 g         3849 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc tatcagaaaa
     9541 tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa tcaggcaact
     9601 ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata ggtgatttgt
     9661 tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt tacttgctgt
     9721 agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt atgacaaata
     9781 gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt
     9841 caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat tttcagtagt
     9901 catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa taactacttc
     9961 atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg aaaaatcagc
    10021 aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg
    10081 tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg tcgcaacagc
    10141 ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca cgatttcaat
    10201 aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa ttctctttaa
    10261 tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat ttgttttccc
    10321 agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt gtaacggcaa
    10381 accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc ttacaccatc
    10441 caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca tatgttctct
    10501 ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag gagtaatgca
    10561 aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc cgttttttga
    10621 aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc attctttcta
    10681 ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt catgttcgtg
    10741 ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga tgatttgatc
    10801 atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca aaaagacaaa
    10861 atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt cgattggatc
    10921 cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct ttgccgacta
    10981 caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa tcaaataatt
    11041 cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg aattcgtgtt
    11101 gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca gcgatttgtt
    11161 tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct tcgaggatca
    11221 tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt gcaaggaaaa
    11281 cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga tggtggggta
    11341 agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct tcaaatgctc
    11401 gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg tttggccagt
    11461 ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct tcaaaagaag
    11521 acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag aatatctctt
    11581 ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc ttgtatcctt
    11641 gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag gaaacagcga
    11701 catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg taaccatttg
    11761 atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta ttcgttcaac
    11821 tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc ttgaaatttt
    11881 tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt aaactaagac
    11941 agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata ttattgacag
    12001 agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag aatatattcg
    12061 tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa ctggacaact
    12121 agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt ttgtggaagc
    12181 aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag aagcacctag
    12241 tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat ctttattgaa
    12301 agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag ggatcgacaa
    12361 ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag atgaagcatt
    12421 cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg agttttctgg
    12481 caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac cttctttaga
    12541 agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat ttgagacaga
    12601 agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag aagaaaaagg
    12661 tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca cggcaaaaat
    12721 ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc ctgtaaaaga
    12781 agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat caaacgggat
    12841 cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat ttgtgaataa
    12901 tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga atcgtttata
    12961 aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa ttgtgtcaag
    13021 agaaggatcg gaaaagacga atataacaa
//
LOCUS       DACEMS010000077        13006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEMS010000077 DACEMS010000000
VERSION     DACEMS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.078"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IW735_001900"
     CDS             complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IW735_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1044894.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1258..3099)
                     /locus_tag="IW735_001901"
     CDS             complement(1258..3099)
                     /locus_tag="IW735_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1044895.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3281..5674)
                     /locus_tag="IW735_001902"
     CDS             complement(3281..5674)
                     /locus_tag="IW735_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1044896.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5781..6641)
                     /locus_tag="IW735_001903"
     CDS             complement(5781..6641)
                     /locus_tag="IW735_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1044897.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6700..7221)
                     /locus_tag="IW735_001904"
     CDS             complement(6700..7221)
                     /locus_tag="IW735_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1044898.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7541..8260
                     /locus_tag="IW735_001905"
     CDS             7541..8260
                     /locus_tag="IW735_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1044899.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8286..10877
                     /gene="mprF"
                     /locus_tag="IW735_001906"
     CDS             8286..10877
                     /gene="mprF"
                     /locus_tag="IW735_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1044900.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="IW735_001907"
     CDS             complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="IW735_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1044901.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12620..12976
                     /locus_tag="IW735_001908"
     CDS             12620..12976
                     /locus_tag="IW735_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1044902.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3738 a         2718 c         2310 g         4240 t
ORIGIN      
        1 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
       61 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
      121 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
      181 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
      241 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
      301 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
      361 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
      421 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
      481 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
      541 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
      601 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
      661 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
      721 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
      781 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
      841 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
      901 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
      961 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     1021 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     1081 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     1141 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     1201 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     1261 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     1321 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     1381 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     1441 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     1501 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     1561 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     1621 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     1681 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     1741 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     1801 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     1861 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     1921 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     1981 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     2041 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     2101 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     2161 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     2221 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     2281 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     2341 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     2401 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     2461 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     2521 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     2581 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     2641 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     2701 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     2761 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     2821 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     2881 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     2941 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     3001 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     3061 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     3121 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     3181 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     3241 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     3301 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     3361 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     3421 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     3481 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     3541 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     3601 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     3661 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     3721 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     3781 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     3841 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     3901 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     3961 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     4021 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     4081 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     4141 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     4201 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     4261 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     4321 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     4381 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     4441 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     4501 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     4561 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     4621 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     4681 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     4741 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     4801 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     4861 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     4921 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     4981 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     5041 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     5101 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     5161 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     5221 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     5281 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     5341 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     5401 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     5461 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     5521 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     5581 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     5641 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     5701 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     5761 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     5821 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     5881 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     5941 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     6001 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     6061 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     6121 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
     6181 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
     6241 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
     6301 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
     6361 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
     6421 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
     6481 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
     6541 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
     6601 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgc actccttctt
     6661 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
     6721 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
     6781 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
     6841 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
     6901 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
     6961 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
     7021 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
     7081 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
     7141 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
     7201 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
     7261 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
     7321 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
     7381 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
     7441 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
     7501 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
     7561 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
     7621 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
     7681 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
     7741 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
     7801 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
     7861 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
     7921 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
     7981 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
     8041 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
     8101 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
     8161 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
     8221 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
     8281 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
     8341 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
     8401 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
     8461 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
     8521 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
     8581 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
     8641 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
     8701 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
     8761 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
     8821 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
     8881 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
     8941 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
     9001 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
     9061 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
     9121 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
     9181 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
     9241 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
     9301 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
     9361 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
     9421 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
     9481 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
     9541 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
     9601 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
     9661 tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat tttatttcct
     9721 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
     9781 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
     9841 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
     9901 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
     9961 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
    10021 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    10081 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    10141 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    10201 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    10261 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    10321 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    10381 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    10441 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    10501 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    10561 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    10621 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    10681 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    10741 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    10801 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    10861 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    10921 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    10981 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    11041 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    11101 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    11161 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    11221 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    11281 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    11341 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    11401 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    11461 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    11521 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    11581 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    11641 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    11701 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    11761 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    11821 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    11881 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    11941 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    12001 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    12061 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    12121 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    12181 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    12241 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    12301 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    12361 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    12421 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    12481 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    12541 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    12601 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    12661 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    12721 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    12781 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    12841 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    12901 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    12961 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaa
//
LOCUS       DACEMS010000078        12967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEMS010000078 DACEMS010000000
VERSION     DACEMS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.079"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(260..979)
                     /locus_tag="IW735_001909"
     CDS             complement(260..979)
                     /locus_tag="IW735_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1044903.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="IW735_001910"
     CDS             complement(1028..2674)
                     /locus_tag="IW735_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1044904.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="IW735_001911"
     CDS             2853..3443
                     /locus_tag="IW735_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1044905.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="IW735_001912"
     CDS             3535..5040
                     /locus_tag="IW735_001912"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1044906.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IW735_001913"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IW735_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1044907.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="IW735_001914"
     CDS             complement(6476..6994)
                     /locus_tag="IW735_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044908.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="IW735_001915"
     CDS             complement(7010..7336)
                     /locus_tag="IW735_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1044909.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="IW735_001916"
     CDS             complement(7349..9328)
                     /locus_tag="IW735_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1044910.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="IW735_001917"
     CDS             complement(9349..9663)
                     /locus_tag="IW735_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1044911.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="IW735_001918"
     CDS             9831..10124
                     /locus_tag="IW735_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1044912.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="IW735_001919"
     CDS             complement(10202..11314)
                     /locus_tag="IW735_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1044913.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11467..11691
                     /locus_tag="IW735_001920"
     CDS             11467..11691
                     /locus_tag="IW735_001920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044914.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            11701..11880
                     /locus_tag="IW735_001921"
     CDS             11701..11880
                     /locus_tag="IW735_001921"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044915.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="IW735_001922"
     CDS             12074..12244
                     /locus_tag="IW735_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1044916.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>12967)
                     /locus_tag="IW735_001923"
     CDS             complement(12668..>12967)
                     /locus_tag="IW735_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1044917.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4088 a         2609 c         2204 g         4066 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actggtgtcg
    12901 catccaattc ataccccgtt ggcgcttgag tttctaccag ctgatatgct cctggtgcta
    12961 agccatc
//
LOCUS       DACEMS010000079        12940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEMS010000079 DACEMS010000000
VERSION     DACEMS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.080"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(29..571)
                     /locus_tag="IW735_001924"
     CDS             complement(29..571)
                     /locus_tag="IW735_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1044918.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            706..1095
                     /locus_tag="IW735_001925"
     CDS             706..1095
                     /locus_tag="IW735_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044919.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1099..1863
                     /locus_tag="IW735_001926"
     CDS             1099..1863
                     /locus_tag="IW735_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1044920.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1992..2939
                     /locus_tag="IW735_001927"
     CDS             1992..2939
                     /locus_tag="IW735_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1044921.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3069..3809
                     /locus_tag="IW735_001928"
     CDS             3069..3809
                     /locus_tag="IW735_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044922.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4100..4768)
                     /locus_tag="IW735_001929"
     CDS             complement(4100..4768)
                     /locus_tag="IW735_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1044923.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4902..5189
                     /locus_tag="IW735_001930"
     CDS             4902..5189
                     /locus_tag="IW735_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044924.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5605..7143
                     /locus_tag="IW735_001931"
     CDS             5605..7143
                     /locus_tag="IW735_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1044925.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7140..8105
                     /locus_tag="IW735_001932"
     CDS             7140..8105
                     /locus_tag="IW735_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044926.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8092..9414
                     /locus_tag="IW735_001933"
     CDS             8092..9414
                     /locus_tag="IW735_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1044927.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9477..9581
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9655..10398
                     /locus_tag="IW735_001934"
     CDS             9655..10398
                     /locus_tag="IW735_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044928.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10413..11405
                     /locus_tag="IW735_001935"
     CDS             10413..11405
                     /locus_tag="IW735_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1044929.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11417..12154
                     /locus_tag="IW735_001936"
     CDS             11417..12154
                     /locus_tag="IW735_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1044930.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12144..12866
                     /locus_tag="IW735_001937"
     CDS             12144..12866
                     /locus_tag="IW735_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1044931.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         4309 a         2202 c         2681 g         3748 t
ORIGIN      
        1 gatattagac agtttttaag tttgtgagtt attttccttg tcgttttctc acttttttag
       61 tccagaaacc acctgaaata tattttggct cataagaaat gataaaagca cgctcttcga
      121 tttctttgat caggcgatac agttcacgtt cgctttttct aggagaaagt atttcgagaa
      181 ctagccgctc accttccagc cccattgcag aagtctgtgt cacaccataa ccgtgttctc
      241 tcagtatacc cggcaaactt ttatcttctg cgctatttgc tggcaaaatc gttgtgacca
      301 tgatgtatcc taatgccaat ttgtcttcaa tcaaaattcc gacactgata cctactgcgt
      361 aacctaacgc atagacaaat aaattcagtg gattatctaa acgatctaaa acaagggata
      421 agccgacgat ataaatcgtg atctcgacca tactgacaag tggagcaatc agtcgatagc
      481 ctttcattgt caacatgaaa cgtaacgtgt tcagagtgat ataagcaaaa ttaatgagga
      541 agatcatccc caacattttt ggatcaatca tttgtaaaac tcctttcatt tatataacaa
      601 caatatcaaa aaaaatcgta agtgaagaaa aattgaaact ttatacatac aaaaaaatga
      661 taaaatgatt ataatagaat tataaagata ggttaggtga ttaaaatgaa aacggtcaat
      721 cgtgtgtttt gtgccagcca cgtgctaagt ctccttctca gtatatactt aggtgtctat
      781 tcgattcaac agccaattga ggtagagaac attgtttttc agatgtcgct gacagatggt
      841 ttaattttaa gcgttttttt ctttttgata ctgtttattg gtaatatttt cggtagcgtt
      901 gccagttttt tgaatttttc tatttatcca ctcctttcgc tagcattagg agtggtggga
      961 ctttgttctc tgtgcttatt tcccgagcca ttcagtcctg catttatatt atttggtaca
     1021 ctgaacctgt tccagacaac tgttggagca tggttgctat ggcgatcagg aaatttaatg
     1081 aaaataggtg agtaacaatt gaatccaatt atattgaaac gttctgtcca tcatattcca
     1141 gtgttagaag ttgttctgaa agaattaaga gaggaacgta ttcccgttgt catctattat
     1201 cacggatggc agacaagtaa ggagttagtt ttgacgcaag gaagaaaaat tgcgcagcag
     1261 ggcattcgtg tgatcctgcc agatgcgatg aatcatgggg agcgcaaaca gccggtttct
     1321 tctattccat cctatacatt ttgggatagt atctacggaa atctgtttga atttgacaca
     1381 cttatcaagc atttagaaaa aagaggtctt ctacatgatc agcttgcagt tggcggggtt
     1441 tcgatggggg gaatgacaac atgcgccttg ttggctaaac atcctcatat agtagcgggc
     1501 gtgtgtttga tgggttcccc tgcacctttg gaatatggaa aagaaatcgt aaggcgcgca
     1561 tcggaatata attatgtcgt accaaaagac tattttgatt tgatttcatg ggttaagagc
     1621 tatgatttgt cattgcagcc ggaaaagtta gctggacgtc ctctgttctt ttggcatgga
     1681 caagctgacg aaaaaatccc ttatatacaa acggaacgat ttgtagaaaa aaatcggcat
     1741 aagtcttacg gaaaagaaat tgtttttcgt tcctcaaaaa cacagcgaca tatggtacaa
     1801 ataccactaa tggaagaagc ttccactttt ttagcaaaaa atttgataaa aaagtataaa
     1861 taattgaaac ggaaaatcac tcatgacgat agaggatttt ccgtttttta ttttgggcta
     1921 taataaactc tgttaggaaa atttggtata attgatagta agaatgacaa catgttagaa
     1981 aggaagagag catgcccact ttagcagatg ttgcaaaacg tgccaatgtt tcaaaaatga
     2041 cggtctctcg agtcatcaat catccggaac aggtgactga cgaattgaaa gagttagttt
     2101 tctcagcaat ggctgaactg gattaccgtc caaatattgc tgcaaaagca cttgtcacga
     2161 atcggtcgca gatcatcaaa ttatttatac tggaagaaat cgacacaact gagccctatt
     2221 acatgaattt gctgatggga atcgctaaac ggatcggtaa agcgcattat tctctccaat
     2281 tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg atacatcatc acaggtgtca
     2341 gaaaaaaaga cttcgattgg atcgaccgtt tggaaaaacc agttgtcttg tttggggaaa
     2401 attctgaagg atatgattat gtagacagcg ataatgaata tggtacagca aaagcaacgc
     2461 aatatgcctt agaaagagat tatcaaacga ttatttatat cggtatcgaa gtggacgagc
     2521 cttttgaaat ttcaagggaa actggttatt tatctgtgat gaaggaagtt gatcttccta
     2581 gtaaaatctt gcggtttcca aatcattcga cagaagtaga aagatatatg acagaaaact
     2641 gggcagattt tacagataaa acttgttttg tatgtagttc agatcggcta gcactaggag
     2701 cagtaagagg gattataaat gctggaggac acttgcccga agaatttggt gtgattggat
     2761 ttgatggggt atttttagac caaatcagtt cacctaaatt gacaacgatc aagcaagata
     2821 ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa aaaaatagaa gaaaaagggg
     2881 cgagtcaagg ttatcgtcgt ttttttcctg aactagttat cagagagaca acgaattaag
     2941 tcttattact atatatatat ttatgaaatt catcgctatt ttcttaaaat aatcagcgca
     3001 ttgctactga aaacaatgaa tctgtgctat tattgagaaa agttccattt ttgagaagga
     3061 tggtttgaat gaaagaaatc agacgaaaac atttccgttt tcctgcgtac gatgatgagt
     3121 taggcgtaaa attaaccaac gacaagaagc gagtattatt tgacggacaa gaagatcttt
     3181 tgactgaaca gaatcagtcc ccgtttgaac agatgaaaga atttcatttc acgaacacga
     3241 acaccgagaa aatccgtcgc aagcgtcgtg caacaaatgg attgaagtca gctccgcgta
     3301 aggaaaattt ggaaaagcat aaaggaaaat taccggatta taccggccat taccaatcag
     3361 aaatcacatc gacaggacga aaaaaacttt ttggtgattc ctctcgttct gtcagacaaa
     3421 gtgaaacaag gacagagtcc aagcatacta cacgaaacgc atctgttgac acgaaggtgc
     3481 gggagcgttc ttattttgtt cctaaatatg tacccgcatc gattatccca gaccctagag
     3541 aaagcaaaat ttctgaggat catttaatcg aatcgatgaa aaaaacggat gactcttatc
     3601 tgttgttcga tacagaaccg acgccgtatc aagaaaaaag aaatggtgag ccgtctgtac
     3661 gaacattcaa tcgaacaaaa acgttagagg aaacaaagca gtcagaagaa aaaaaatcga
     3721 aaaaaagcca tggtatctta gaacgctcat tacaaggatt gatcgatgat caaggaaact
     3781 ctttgggaga aaatagctat tttaaataag tgatatacaa atttatattc ggctacagtt
     3841 ggaaaggaaa cgctgcggcg tttccttatt ttatactcta tccgaaagtt tatatcgtat
     3901 cctcaaattt ttttgagttt aagcgaattt tttctcccaa taagtaagaa aataatggca
     3961 aaataaaaaa acaaactatt ttcgcagatc gtttgcaaaa atagtttatt tttttataaa
     4021 gaagatttta ctgaataata tcattttagt ttacataatg atatttctgt aaattaaata
     4081 aagtagtgaa aaataagaat cagtgctcta actgttcaag aggatcaagt ggttcaaatg
     4141 tttctgtagt ctgttttgct tccaattggg gcaaaacaga tccaatttcc agatacttca
     4201 ctttattcaa tgtataagat agaatagaac agctaagtaa tgtatacaga aaatgtgtga
     4261 gatccaaata aagcaaggag acaactaaaa taccaaaagt aatcaagaaa agacttttgc
     4321 caatcttaat atgaaaatat ttttcaatgg cttgataaat gatatctgtt ccgcctgtgc
     4381 ttccgccgaa tcgaaagacg agaccgcaac caatgccaga aagaatacct gtcatcaaac
     4441 tattgatcag tagactatct acagcaaaat gtatatgaag gctctcccat aaatgtaaaa
     4501 agaaaactag cgcaaatgtt ccgacgatcg tccggagaaa agcgtgtttt ccaaaaagaa
     4561 acaatgagat aagcaataaa ggtaaattaa aaagcagtga agtgacagaa ggagcgaatc
     4621 caaaaagatt atgcaaaagc aaagcaatac cagtaacccc accatctgcc agcttagatg
     4681 gaatattgat tcctgtcaca gctaatccgt agattcctgc gcctaaaatg attccgcaca
     4741 attctgtaaa taaatttaat tttttcattt ctagcctctt ttcttttgaa aattttcagc
     4801 aacttcttta ttatactgac ttatactctt acttcaagag gaaaatgaaa aaaaattatt
     4861 tttggctgtt tctttttatt tatcaaataa ggagtggaaa aatgcgagca gcagacttat
     4921 tgacaattct tacaaaatca cccaaaaaaa cagagtatca aggagttttt ttggctcaag
     4981 aacaaaaact ggtgcctgtg acaatggtcc agataaataa agcaaatcaa tttatctttt
     5041 tatttgaaca cggcaaagca ccacttgcta tgaaagacat ctttcttttt ttgatgaaga
     5101 accgtgagaa agctgtttat tatcagtcaa aagaagaact cattcctctt tatggagtga
     5161 aagagataga aaataaagtg gtgatataag taaaaataag aaaacaagac gtttatgatg
     5221 tcaaaacaac cattttttac atttttggca aaaaagtgtc atgatacagt ataatattcg
     5281 ttgaacaggc caataaaggt gttctccccc acgagatttc tactttattg gctgtttctt
     5341 tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc tgattccaag tgacaatttc
     5401 gtatagattt ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg atccgacttt
     5461 gttgcttgga ttcttttttt gtcagcatat ttagaggtat ttttttcttt tttttgttcg
     5521 tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg tttatactta
     5581 aagaataaca gaaaggaagg tcgcatgaat aattttatac aaacattaag tgaaagaaaa
     5641 ggcgagttgc tggaagctgc atttcaacat ctgtccattt ctctcatttc tttgctgatt
     5701 gcagcactga ttgctattcc tttagcgatt tgggcagcca atcataaaaa aatggcagaa
     5761 gtcttattgc agatcacggg tgtgctccaa acgattcctt cacttgcctt attaggttta
     5821 cttatcccat ttgtagggat cggaactgtt ccagcattga ttgcacttgt tatttacgct
     5881 ttgcttccga ttttccaaaa cacgtatatt ggtctagctg aaattgaccc gtctattgaa
     5941 gaagcagcgg tagcatttgg tatgtccaga atgagacgct tgctgaaagt agagcttccc
     6001 atcgcattac ctgtcattat ctcaggtatc cgaactgcct tagttttaat catcggtacg
     6061 gctactttgg cagcattgat cggagcaggt ggattaggga catttatttt gttaggtatt
     6121 gaccgaaata cacctgtttt gacattgatt ggtgcgatca gttctgcctt gcttgcaatt
     6181 atattcagcg gactgattcg ctggttgcag cataagaaac ctcgatattc catcattact
     6241 ttagcagcag tggtgtgttt atttggcggc gtttctttat atcagtctgc cttgtttaaa
     6301 aatgaaacca tcacgattgc tggaaaatta ggagcggaac ctgaaatcct gatccaaatg
     6361 tataaagaat tgattgaaga caacacaaaa acagaagttg aattaaaacc gaattttggc
     6421 aagacgagtt ttttgttcaa tgcactggat caaggtcaaa tcgatatcta tcctgagttc
     6481 acaggtactg tcctagaaag cttggtgaag gtacctgaag caatgaaaag tgctgatttg
     6541 acaaaagaaa aaacatatga cgaagcagaa gaattattga ataagcaatt cgatatgaca
     6601 ttattaaaac caatggctta ccaaaatacg tatgccttgg cagtcaagag atcttttgcg
     6661 aacgaacatc aacttaaaaa gatttccgat ttggcagcta tctctgatca gatcaaagcg
     6721 ggattcacac tggaatttat tgatcgttca gatggatata aaggaatcca agaagcatat
     6781 ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt tgcgttacca agcaatcaat
     6841 aatggagatg tcaatattgt ggatgcctat tccacagata gccagctcaa ggagtacgat
     6901 ctagtgactc tagaagatga tcgagggtta ttcccagctt atcaaggcgc accgctgatg
     6961 aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag cactgaataa attaagcgga
     7021 aaaataacag aggatcaaat gattgatatg aattaccaag tcaatgtcga aaagaaaaag
     7081 ccgacagaag tagcacgaat cttcctgaaa aatgaaggat tagtaaagga ggaagatcag
     7141 tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag tatgtcatcg
     7201 atgacctaaa tctggcaatc aaaaaaggag aaatctttgt tttggttggt ccttccggta
     7261 gtggaaaaac aactactttg aaaatgatca atggattatc taaaccatca gcaggagaca
     7321 tctacttcaa aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg tggaatatgg
     7381 gatatgtgct gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa aatattgaag
     7441 tcatccctga aatgttaggt tgggaaaagc agaaaagagc agaccgtgta gatgaacttc
     7501 ttcaaaaagt tggcttatct cctgatattt atcgagatcg tatgcctaga gagttgtctg
     7561 gaggagaaca gcaacggatc gggatcatcc gagctattgc tgcaagccca gatgtgattt
     7621 tgatggacga gccatttagc gcattagatc cgatttcaag aaactcatta caagaattgg
     7681 ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt cgttacgcac aatatggaag
     7741 aagcaatcaa gctaggtgat cgtattgcat ttatgaagga tggagaaatt atccaatgtg
     7801 acacaccaga gcagttattg atgaatccta aaaacgacta cgttcggcat ttctttgatg
     7861 aaccgaaaca gacgaaagag tggcgagtag aagatttagt cgtcaatgga tatttcttaa
     7921 atgaaatacc agaaaatgct cgtcaacaag tttgctttga tacgcctatg aaagaagtat
     7981 attcgcttct ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga agtattgggt
     8041 cgctgacaag taaagaaatc tttgcttttt taagtcgaga ggaggaagga aatgaaaaca
     8101 tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta tggattgaaa
     8161 gaagcaggta aaacagtact agtggtcgaa gaagatctat ggggcgggac ttgtcctaac
     8221 cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa tcgggtaaaa
     8281 caattgagtg gaaaaggttt taacgagatc ccaactgcca actgggaaga acttcaaaaa
     8341 ttcaaacgaa catttacgga tccagtccca gaaagccgaa aaaaacaatt agcagaagct
     8401 gaaatcgatc atctttcagg aaccgctcgt tttttggatg attcatctat tgaagtcaac
     8461 gaagaagtct tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc tacgatctta
     8521 ccagtggagg gaaaggaata cttgaaaact agtgcagact ttttatcatt gcctgtactg
     8581 cctaaagaaa tcatttttat cggtggtggt tatattgctt ttgagctagc tacgattgcc
     8641 aatgctgcag ggagcaaagt aaccattgtc catcataatc aacggccatt aaaagaattt
     8701 gaggcgtctt tggtagagga agccgtccat caaatggaag catctggcat tcagtttgct
     8761 tttggtgttg aaacccaaaa gatcatttca gaaggtactc gctatcgttt agtcggaaaa
     8821 gagacggaat tagtagcaga tatgattttt tgtgcgactg ggagacagcc gaatacagaa
     8881 tctttagcac ttgagcaagc gaatatcgtc tttgataaac acggaattgc agtcaatgat
     8941 tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag atattgtttc aaggaaaacc
     9001 ccaaaattga caccagtcgc cacttttgaa ggaaattatg tagctaagcg gataacggat
     9061 gcaacaagcg aacctatcaa atatccgatt atacctacga tcgtctacgc cagtcctaaa
     9121 cttgctgaag taggagttac aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg
     9181 gatcttacca gttggttcac ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg
     9241 acctttgacc agcaaaatta tttgattggt gctgcagtga ttagcgagca agcagacgaa
     9301 ttgatcgatg atttgacact cgtcatcaat caaaaattga ctaaaaaaga gttggatagc
     9361 tatatcatgg ggtatccgac attggcaagt gatctctcct atttattgaa ataatcagca
     9421 tgataactaa agcgcttgtt gctttttcct tcttgtgcta cactttaaaa aatgaatacc
     9481 tgtttctggg gatgtcgtag acgacatgag agtaagttgt gaacttagac ccattgaacc
     9541 tgtcagttaa tactggtgaa gggagaaaca tagcagacgc ttgaagcggg ttactatgtc
     9601 gtgcccattt caagcgtttt ttgttattca tattagaaga aaggaggtaa caaaatgaag
     9661 aaaaatcttt atccagcagt cagtatggca ttcattttaa ttatttggca gattgttgtc
     9721 atagtttgga aaatacctgc ctttatttta cccggacctg ctgctgtatt ttctgcatta
     9781 gcggcagatg cacaagtatt actttcccat tctgtcacga cattaaatga agcagtgctt
     9841 ggtttggtaa tagctagcgt tctttctttt attactgcct tggcaatgga ttattggaag
     9901 tttttagaaa tgtcttttta tccgttacta gtgatttcac aaatgttgcc aatcatggtg
     9961 ttagggccat tacttaccct atggtttggt ttcggtatgc ttccaaaagt gatccttgtt
    10021 gtcttgataa gctattttcc gatcgtagtt tctttttcgg atacattgag gaaagtatca
    10081 aaagagcaaa tcatcttttt gaaaacgatg ggggcagata cagggaagat ttatcgaata
    10141 tataaaatac cagcgggttt atccggcttc ttttccggtt taaaagtggc tgctacttat
    10201 tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt agggtattac
    10261 atgatacgcg taaaaaacag ctaccagatc gataaagttt ttgctgcgat catttgtgtc
    10321 attctattga gtttattgct gaatggcgct tgttcattat tgaaaaaagg gtattatcgt
    10381 atgctttata ttttttaaga gaaggggatc aaatgaaaaa attatggctt ttattaccgc
    10441 tactgttgct tttatccgct tgtggcactg caaaagaaac aagtagcaaa caagaaataa
    10501 aggatttgaa aaaagtgaca cttgttttgg attatgtacc gaatactaat catactggca
    10561 tctacctagc aaaagaaaaa ggctattata aagaggctgg tttaaatgtc caaatcatcg
    10621 aaccaggaga taattcgaca agtattggtc tagtaggtgc ggacaaagca caatttggtg
    10681 taagttatca agaagatgtc acttatgctc acgcagatgg acagaatata ccagtcaaag
    10741 caattgctac agtgatcaaa cataatactt ctggattcgc caccctatca gacagtaaca
    10801 tccattcacc aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag
    10861 aagctgtctt aaaagctgtg atggaaaaag acggcgggga cttttcaaaa ctgacaatgg
    10921 taggaagcaa tggagaaggg ccagaaagct taggaaaatc ctcagatatc caatggtact
    10981 ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat tgaactaaac tatatcccat
    11041 tgaaagaatt agatgaacgg ttagattact atactccggt aattattacg aatgatcaac
    11101 tgatcaaaag tgatccagaa ttggtacagt cttttatgga tgccacgaaa aaaggctatc
    11161 aggaagctat taaggatcca aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa
    11221 atgatcggac gtttttagaa gaatcacaag cattcttgtc aaaaaattat acggatgatc
    11281 ctgagaattg gggattgatg gaagaaaaag tttggcgcaa ttatacagct tttatgcagg
    11341 aaaacggatt gatcaagcaa gatgtacctt cgcaagaact attcactaat cagtttataa
    11401 aataggagtg atagatatgt caatccaaat cagccacttg aatgtagtca ttgaaaacct
    11461 gaaaatatta gaagacatcc atttcactat ccctaaagat acctttacat cgattgtcgc
    11521 gccaagcgga gccggtaagt caacattgct gaaaacttta acaggagtcc agcctatgac
    11581 ttctggaacg atcaaagtag acgatcaaaa agtaactggt ctccatacag catttagtta
    11641 tatgccgcaa gaagatatgt tacttccttg gctgagcgtc tatcaaaacg ttacgttata
    11701 tcaaaaaatc aatcatctaa caatcaatga agaacaagta agggattatc tggatatctt
    11761 tgggttaaac ggctatgaac attttttgcc ggaacaatta tctggcggga tgaagcagcg
    11821 aacggccttg ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt
    11881 tggagcgtta gatgctatga ccagagggca gatgcaagac tggatgttga agctgcctaa
    11941 aaaagcaaag cggactactt tacttgtgac gcatgatatc gaagaagcaa tttatttatc
    12001 agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc
    12061 tgaaaaacga cggtcacgtg aatggctact gcagcaatca gagttgaagc aaacgattta
    12121 tcagttattg gcaggtgaat tagatgttaa gtgatgctca ttgtcattta gatgggagtc
    12181 agagtttagc actccttcag caggaacaca cgattttgac gatcatcaat tgtgattcgc
    12241 cagaagaatg gaaggaaaac agacagcttg cagcaagtaa gactcaagcc ttaagttacg
    12301 gtatccatcc ttggaaagca gatagctata cgtttgaaca agttgagcct tttttgaaaa
    12361 aagcaaggat catcggtgaa atagggttag ataatatttg gacgaatgta ccgatgacta
    12421 cacaaaaaaa agtatttgag cgacagcttg cttttgcagc cataaatgag aaacctgttg
    12481 tactgcacac aaaaggatgc gaaaaagaga tcttaaatta tatccaagct taccctaatc
    12541 actatttgat ccattggtat tcttctttgg actatcaaca agactatatt gatttaggct
    12601 gttatttcag cattggtctt gatttgaaaa agaatccagc agtctggcaa ctagctaaag
    12661 cagtcccaat cgaccactta cttattgaaa cggatggact acatgcagca gagtgggctt
    12721 taggggggat tttttcagaa aaagattatg tctcactgct tgaagaacag ttaaagacaa
    12781 tcgctgagat caaagagcta tcagttgaag aagttaaaaa aaatacttat caaaacttgg
    12841 aacgtttcat tagattaggt aaataaaaaa gagatgtgac acaactagtg tcgcacctct
    12901 ttttggcttt ctttgtataa aagtaaactg tttgattaat
//
LOCUS       DACEMS010000080        12687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEMS010000080 DACEMS010000000
VERSION     DACEMS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.081"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(113..556)
                     /locus_tag="IW735_001938"
     CDS             complement(113..556)
                     /locus_tag="IW735_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1044932.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(572..1732)
                     /locus_tag="IW735_001939"
     CDS             complement(572..1732)
                     /locus_tag="IW735_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1044933.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2239..3939
                     /locus_tag="IW735_001940"
     CDS             2239..3939
                     /locus_tag="IW735_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044934.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(4041..4874)
                     /locus_tag="IW735_001941"
     CDS             complement(4041..4874)
                     /locus_tag="IW735_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1044935.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5178..7343)
                     /locus_tag="IW735_001942"
     CDS             complement(5178..7343)
                     /locus_tag="IW735_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1044936.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7603..9897
                     /locus_tag="IW735_001943"
     CDS             7603..9897
                     /locus_tag="IW735_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1044937.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9890..10555
                     /gene="pgmB"
                     /locus_tag="IW735_001944"
     CDS             9890..10555
                     /gene="pgmB"
                     /locus_tag="IW735_001944"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1044938.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10548..11555
                     /locus_tag="IW735_001945"
     CDS             10548..11555
                     /locus_tag="IW735_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1044939.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11577..12596
                     /locus_tag="IW735_001946"
     CDS             11577..12596
                     /locus_tag="IW735_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1044940.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         3819 a         2668 c         2268 g         3932 t
ORIGIN      
        1 tatcaaagat tgtaacaact gcatattcac acagttaaat tagtgacttc aaaatgaaat
       61 aatgctccca gcaaaaaatt aggctgggag cattatttcg ttattctgtt catcaaaaat
      121 cagcgttttg attccatttc atcacaagtt cctcctcatt tactgcttca tctttttgct
      181 gtgtttgaat tgtaaaacct tgtgaaagat agaaatgaat agctgattga tttttttgat
      241 agactgtcaa agtcagtgtt ttattggttt cttttatttt tttcattagt tgcgtaccga
      301 ttctttgttg ccggtattcc cttttcacaa agattcctgc gataaactga tcaatcaccc
      361 cagcaaaacc aataatttct tgatctattt ctgctaagta aatcactgca tctggtaaag
      421 ctttcctaac agattctact tgattctccc aataagttga tggaatgaat tcatgagaat
      481 ccaagttagc atgcagccaa atcgatagaa cagattccag ttctttggca gacaattttg
      541 ttgttgtgcg gatcattttt tctctcctta cttattcatt gattttaaaa taaaaaccat
      601 ttttttgaag ctcttttaca agttttcgcc cctcaaaaaa gtatatcttc tcttgtttta
      661 cttttctaat accaaaagaa acagcaactt taggatcatc agctgaaacg attcgataag
      721 ttccaccagg caattgatcc agcttgactt tcccatgatg gtcgctttgt actgaaaacg
      781 gcacaccatt tctagtaagt gtttttttct tatgtttcaa aatgaatgaa ctaccattta
      841 ccggtttccc tgacgaatca ctaataataa atgaaaaatc aagcactttt ttacttaatc
      901 tatactgata gattagtcgt ccgataaaca ctaacagtat gactacagct attatcgttc
      961 ctgcaagaat aaaccattgc cgcatggttc tttcctgatt tccttttcct tcttcttttt
     1021 ttaccgattc gttataagga atccgatgtc ctgtcactag caaacgatgc gtgttgatca
     1081 tatacggcgt acatgtaagc aaggtaacta agtcttctcc ttctgaaatc gttagaacag
     1141 acgtgtcatc tggcagtacc acttgtatat ttttaacttg ataagccaat ttctccccca
     1201 aaacagtcaa aataaacgtg tctccgtttt ctaaacgatc taattcggta aataaccgtc
     1261 gattaggtaa accgctgtga gcagagatca ctgaatgcgt cgaatttcca ccaagcggga
     1321 aagaggttcc ttgcaagacg cctgctccat tttcaaggat cctaccattg agccgatcat
     1381 aaagtgaaat atttaattgg atcttaggta tattgatact tccgatcagc cattcctcta
     1441 attggctgga tgttgtactt tcatttctgt tcttttcatt gaaaggatca gtaccaggat
     1501 tgaatccttg gtcagttaat cgttggtttt tctcctccat tttctttcgc tgcacttcaa
     1561 tttcagctgc ggtccgtttt tgtacttgtt cgatacgttt ttgatcaata aaactattta
     1621 aagaatccac ataaaatgga tataaagcga tcagtaaccc tacgacgata catattaata
     1681 aaaacaaacg aaacaaaagt cgacgagact gtttttgctt actctgcttc acatgtgtcc
     1741 tcccttgcaa gtttttttgt tctttctttt attatatggg ttcaggaaaa aaatagaaaa
     1801 tgaaagccga tgaggttgtg aaaaagctgt cctgcatcaa gtaataagta cagcccaaaa
     1861 aaatagctac tcatattttt tgttgggttg ggcttattac cgcagatgca agcttttgaa
     1921 caccgtttaa ggaagaggtt gtgaaaactg cgttatgctt tgagtaataa gagcgctagt
     1981 cagaaaatag cttctcatat tttctaggct agtgagctta ttaccgagaa gcagctgatt
     2041 gaacaccgtt taccgagagg tcatgaaaaa gctactttgt ttcgagtatt aagaaaaatt
     2101 ctgtttcaaa acaaggagat gcaacaaaac ttatgtcgca tcctctctcc ttagaacttt
     2161 cacttatgtt tttcttgtaa aattcataaa tattttcatg aattttctaa tttcttgcct
     2221 acctctcgca taggatatat gatgaattta tctaaagttt gcaaaggagt tcgacaattg
     2281 aaaaaatatg gcccattttt agcaattttt ttcattgctt tggcatttct catgcctcat
     2341 ttattcacac ataaactaat tttgggtgct gatgccccat tccattacaa tcggttctat
     2401 gatgccgcgc aacaaattaa atatggggat ttccattatt ttccttccct ttatggattc
     2461 cagcaaagtg gcaggatcgt gaatgccgtt tacggcccat ttttcgctta tttacaagga
     2521 ttgatcgtgt tgcttgcagg tagctggttt cattatcaga ttctttcaaa tttcattctt
     2581 tatcttattt caggattttc tatgctgatt ttactgagat atgtcaatgt tcgaaattgg
     2641 gtaagtgtac cagtagccgt tttattcatg agtacgtatt ctattcaata ctggtctatc
     2701 aaccaaggat tcactagctg ggggacttgt ttttatcctt tatgtatgat ccctattgct
     2761 gatttcgtcc ttcataaaaa gttccctgtt atcaaagtcg ctgtctcagt gggacttatg
     2821 acacagatcc atttactgtc ttcgatcatg ttagctttga tgtatatccc tttttatctt
     2881 tattactttt ttaaccagac tgaaaaagga aaagcactgc ttcaactgat taagtcggtc
     2941 ttgctttatt ttgttctgac agcaaatatt tgggcaggat tagctattgt ttacagtggg
     3001 aataagattc ttcctccctt tgttaacaga gaaatgagtc aaaaggcgat agatctggat
     3061 ggcagttatt ggctccatta ccctaaggcc tttcctatat tgatattatc aggttttttg
     3121 ctttttattt tctttcaaca aaagaacacc ttattccaaa aaatcgtgtt gtacacaagt
     3181 cttgttttct tttttctttc tactagcttg attccatgga cgacattgat cgaaaaaaat
     3241 gtgcctttta ttagtttgat ccagtttcca tttcgatttt tcgtgccatt tacgctattg
     3301 cttctacttt accttgccct ttcactgaac cagtggtctg aacaaaagtg gttccgtctt
     3361 ttaagtatat ttggcattct tattttttca gcacagacga tccagaactt ataccagcat
     3421 ctggaaaagt gggaaaatga aacatttgtc agtcgacata cttatctatt cgatacgccg
     3481 gaagaagcaa ggaaaacatt cttcagcaaa gatatgtcta ccagcttgtt cgttttccaa
     3541 aaaaccacac ctgactatct gccaatctat aaggagacaa aagacaataa atacgaccgg
     3601 tataacgaag aaatactaga aaacgaaaca acttttataa aaacacatcc aggcggtctt
     3661 ttgacaatca agtggaaaaa cactggggac aaaactgttc atatccctgt tatcgtctac
     3721 gatcggactg tgctacagca aaatggaaaa acattgactg attatgaagt aacagatatc
     3781 gggaccccaa tcgtgaagca gcaaaaagga atcaacgagc tcacgcttca ttatcaaacg
     3841 ccaatctatt tctatttcat tctttctctt actttgattg gctggtttac cttgctatgt
     3901 ttatttattt atcatagata caagctttta cgtgcttagg caaaagggat aaatacatcc
     3961 gatcaaaaaa ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt
     4021 tatgttatat gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga
     4081 tcactgatga ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt
     4141 ccttcaggga cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct
     4201 attttctttt ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga
     4261 agaagaaggc aactttctct tacagtataa taaccttgag agtctactgg attattgaaa
     4321 tcacctagga aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac
     4381 tcaaaataaa agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa
     4441 atgtcctgat catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct
     4501 ttcttttgaa attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt
     4561 gcatttcctt cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac
     4621 tcttttccgt gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata
     4681 ggtacttgat tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat
     4741 tccagtaatt ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta
     4801 tctgctaatt cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt
     4861 gtcatgaatt tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa
     4921 atttttgtca caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct
     4981 caatggacca ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt
     5041 ttcacgacct ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca
     5101 aacgaaaaat aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc
     5161 tttttcacaa cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta
     5221 cctaattgct tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt
     5281 ggatcttttt cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt
     5341 cctacttctt ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta
     5401 tcgattccca tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt
     5461 ttgctaggga aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt
     5521 ggaataacag caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca
     5581 gataaaggaa tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat
     5641 tgcttactac ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct
     5701 cctgattgca gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact
     5761 gcttggactc cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct
     5821 tcagtcccta ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg
     5881 acatctttga tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct
     5941 gctgttcctg ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg
     6001 ttacgtcccg gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta
     6061 acaacaccaa aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct
     6121 ttgatagcta acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat
     6181 ggcaagatat ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat
     6241 ggcacagcag caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca
     6301 gccaacgctg ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta
     6361 cgatacatcg caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact
     6421 ttgaaacgag ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca
     6481 cctttaagat tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact
     6541 tgtgtccccg cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg
     6601 ataggaatcg tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca
     6661 aataaaaacg gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa
     6721 ttattgattc cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt
     6781 gaccataaga taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc
     6841 ggcaattttc tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg
     6901 atccctacga atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct
     6961 ttaaccatga tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg
     7021 tcacttgtga caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca
     7081 gctgcaaatg ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat
     7141 aaaatatgaa gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg
     7201 acaagcggcg tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac
     7261 ccagctgccg gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac
     7321 tcaaaactaa atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac
     7381 gtattatagc gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac
     7441 cattctataa aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt
     7501 tttttaagct tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc
     7561 gtttattatg aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga
     7621 ctatttgcta ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga
     7681 ctgcaagaat ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa
     7741 gaaacttact caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat
     7801 aagacacgtg tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac
     7861 gccttgaact ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct
     7921 ttggaaccag aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca
     7981 ttcacaatca ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt
     8041 gtcaaaaaag aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca
     8101 gtcaaactcg tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa
     8161 catttttgga atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa
     8221 actattccaa acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc
     8281 gttaatggaa aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc
     8341 ttgtctgcag aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt
     8401 actagtagag atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac
     8461 cagacaaaca attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg
     8521 aagcttgccg atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat
     8581 ttgttccaac tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa
     8641 ggattcacag gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc
     8701 cctctttatc ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat
     8761 aatcaactgc cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat
     8821 cctatggtca ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag
     8881 attcaccgaa atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat
     8941 acggaatatt tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca
     9001 gaccgtgtcc atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca
     9061 aacgaatatg agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc
     9121 ttgcgttaca cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc
     9181 gaagaagaac tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa
     9241 gaattaggaa tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc
     9301 gatattccca caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga
     9361 tcttgcttca tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt
     9421 acaaaagaag aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct
     9481 tccctttcgc caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct
     9541 gtagaaatgt accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa
     9601 gacggcttgc acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct
     9661 caaatgaaaa ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa
     9721 gcttattctt tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa
     9781 caagttactt tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct
     9841 tattctttag cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg
     9901 ggtattgttt gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg
     9961 gcgaaagctt ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa
    10021 aggcgtcagc cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc
    10081 tttttcaaag gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat
    10141 gatccagacg atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt
    10201 aaaagaagaa aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact
    10261 tgaaaaaatg ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa
    10321 cggaaaacct gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga
    10381 agaatgtatc gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc
    10441 tcaaccaatt ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga
    10501 tacgactttt cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag
    10561 agatctccgt cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact
    10621 acttagaagt cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca
    10681 aagaaggaaa atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag
    10741 acaaaagctt ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt
    10801 ggggtgagca tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag
    10861 gctggtcttt ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg
    10921 tcttttactt aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt
    10981 accgtttaac aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca
    11041 tctgcaatcc gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct
    11101 atgaacatca gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc
    11161 ctactggaaa ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac
    11221 tgcaagagat ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc
    11281 ctaggctttc caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta
    11341 tagcaacttc taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct
    11401 ctgtcaatgg aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc
    11461 ctgatatcgt tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga
    11521 tcagtcagac gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg
    11581 ctattaccgt aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg
    11641 tcatcaacga tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg
    11701 atgaactagg ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg
    11761 ccatcggcgt aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc
    11821 ttgagatcat ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg
    11881 ctactgctaa aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac
    11941 aagtagatgg ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat
    12001 accaaaacca catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg
    12061 tttatgtaga taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga
    12121 atggacataa gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac
    12181 gatatttcgg ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag
    12241 tactagagca tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca
    12301 cagcattggt ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt
    12361 atggctatgt cattccagag acactctccg tcattagctt caacaactcg atcttctcaa
    12421 cattaacaca cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg
    12481 ctatgcaaaa gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca
    12541 tccctcatcg cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag
    12601 ctgactcact aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat
    12661 actacttcta acacattgtt tatctgt
//
LOCUS       DACEMS010000081        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEMS010000081 DACEMS010000000
VERSION     DACEMS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.082"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..305
                     /locus_tag="IW735_001947"
     CDS             6..305
                     /locus_tag="IW735_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1044941.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IW735_001948"
     CDS             378..1739
                     /locus_tag="IW735_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1044942.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IW735_001949"
     CDS             1761..2021
                     /locus_tag="IW735_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044943.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IW735_001950"
     CDS             2035..2787
                     /locus_tag="IW735_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1044944.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IW735_001951"
     CDS             complement(2881..3486)
                     /locus_tag="IW735_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1044945.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IW735_001952"
     CDS             complement(3539..4513)
                     /locus_tag="IW735_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1044946.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IW735_001953"
     CDS             complement(4891..5670)
                     /locus_tag="IW735_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1044947.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW735_001954"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW735_001954"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1044948.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IW735_001955"
     CDS             7161..8558
                     /locus_tag="IW735_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1044949.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IW735_001956"
     CDS             8639..9079
                     /locus_tag="IW735_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1044950.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IW735_001957"
     CDS             9227..9553
                     /locus_tag="IW735_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1044951.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IW735_001958"
     CDS             9554..10303
                     /locus_tag="IW735_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044952.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IW735_001959"
     CDS             complement(10383..10838)
                     /locus_tag="IW735_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1044953.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IW735_001960"
     CDS             complement(11265..11540)
                     /locus_tag="IW735_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044954.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IW735_001961"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IW735_001961"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1044955.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2176 c         2586 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACEMS010000082        12661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEMS010000082 DACEMS010000000
VERSION     DACEMS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.083"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW735_001962"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW735_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1044956.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IW735_001963"
     CDS             808..1242
                     /locus_tag="IW735_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1044957.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IW735_001964"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IW735_001964"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1044958.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IW735_001965"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IW735_001965"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1044959.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IW735_001966"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IW735_001966"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1044960.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IW735_001967"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IW735_001967"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1044961.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IW735_001968"
     CDS             9513..10439
                     /locus_tag="IW735_001968"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1044962.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IW735_001969"
     CDS             10621..11514
                     /locus_tag="IW735_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1044963.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IW735_001970"
     CDS             11648..11773
                     /locus_tag="IW735_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1044964.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IW735_001971"
     CDS             complement(11907..12539)
                     /locus_tag="IW735_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044965.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         4557 a         2135 c         2644 g         3325 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 t
//
LOCUS       DACEMS010000083        12594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEMS010000083 DACEMS010000000
VERSION     DACEMS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.084"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(68..1099)
                     /gene="queA"
                     /locus_tag="IW735_001972"
     CDS             complement(68..1099)
                     /gene="queA"
                     /locus_tag="IW735_001972"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1044966.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1292..2467
                     /locus_tag="IW735_001973"
     CDS             1292..2467
                     /locus_tag="IW735_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1044967.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2471..3112
                     /locus_tag="IW735_001974"
     CDS             2471..3112
                     /locus_tag="IW735_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044968.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3109..4026
                     /locus_tag="IW735_001975"
     CDS             3109..4026
                     /locus_tag="IW735_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1044969.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4023..4694
                     /locus_tag="IW735_001976"
     CDS             4023..4694
                     /locus_tag="IW735_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1044970.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4890..5724)
                     /locus_tag="IW735_001977"
                     /pseudo
     CDS             complement(4890..5724)
                     /locus_tag="IW735_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5925..6191)
                     /locus_tag="IW735_001978"
     CDS             complement(5925..6191)
                     /locus_tag="IW735_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1044971.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6191..6454)
                     /locus_tag="IW735_001979"
     CDS             complement(6191..6454)
                     /locus_tag="IW735_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1044972.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            6929..7114
                     /locus_tag="IW735_001980"
     CDS             6929..7114
                     /locus_tag="IW735_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1044973.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7256..9079)
                     /locus_tag="IW735_001981"
     CDS             complement(7256..9079)
                     /locus_tag="IW735_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1044974.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9676..12381)
                     /locus_tag="IW735_001982"
     CDS             complement(9676..12381)
                     /locus_tag="IW735_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1044975.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3623 a         2625 c         2375 g         3971 t
ORIGIN      
        1 ctttttcttt gtcataaaat ctttgtaaag cttttttgtt attcaaaact agcggctttt
       61 cataacctta tttcacgaac atcgcatctc caaaactaaa gaaacgatag cgctcttcaa
      121 tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta actaacatga
      181 ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc ttctaccact ttgaattcat
      241 agccaggcgt aatgaaaata tcagtccatc cgctgtctgc tttgatttct ccattaaatt
      301 tcgttccaat cgtttctaat gttcgaattg aagttgttcc gacagccacg attttcccgc
      361 catttttacg tacttggttc aatcgctcag ctgcttcttc cgtcaaacgg tagaattcac
      421 tatgcatatg gtgttcttca attttatcta cgctgacagg tcggaatgta cctaacccta
      481 catgtagtgt cagatagact aattcaacac cctttgcttc gatctcatca agtaattctt
      541 tcgtaaaatg caatccagct gttggcgctg cagcagaacc attttctttc gcatagacag
      601 tttgatagcg atctgggtct tccagacgtt ctttgatata tggaggcaat ggcatttctc
      661 ccaatgattc aagaatctct agaaaaattc cttcatactc aaagtcaatg atccgtccgc
      721 cgtgttccaa ttcttctttc actaccgctt gcaatctgcc atcaccaaaa gagattcttg
      781 tcccaacttt ggcacgtttc gcaggtttta tcaatgtctc ccacgtatct ccttgtgtat
      841 tcgttagaag aaggacttct aaatgcgcac cagtatctgg tttttctcca tataaacgag
      901 caggcagaac tcgtgtatta ttcataacta gcgcatcacc aggctgtaac tcttcaatga
      961 tttcatggaa atgtttatct tccatctctc caattttccg atccaaaaca agtaaacgag
     1021 aatgatcgcg gtcttttaat ggtgtttgtg cgatcaactc ttccggcaag tcaaaatcaa
     1081 aatcttctgt tgttaacata tcgtcactca ctttctatca tgtgcttcat cattctatca
     1141 tgtttttcat tctgttggca aaatcagcac tttttcactt ctattttttg ttttcctaaa
     1201 taaaaaaact accctatttg tattttttca ttgttttagg catactagta atagatagtt
     1261 cttcattgaa aatagaaaaa gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat
     1321 ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt
     1381 catttgtttt atcggtacga gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg
     1441 aatgactgat ccaacacaag ggaaaatttt gattgatgga aaatccatcc aagaaattga
     1501 tccagtggaa ttgcgacgtc agatcggcta tgttatccaa agtattggat tgatgcctca
     1561 catgacgatc cgtgaaaata tcacccttgt ccaaaaattg ttgaaagtca gtcaagaaga
     1621 acggaataaa acagctgaaa aaatgattga tcttgtggaa ttgccccgtg agatgttaga
     1681 tcgctaccca catgaattat ctggtgggca gcagcagcgt attggagtcg ttcgtgccct
     1741 tgcagcgaat caagatatca tcttgatgga cgagccattc ggtgcgcttg atcccatcac
     1801 tcgtgattct ttgcaagacc ttgtcaaaga tttacaagag cgcttgggaa aaacgatcgt
     1861 atttgtaacc catgacatgg atgaagcgat taagctagca agccgtatcg ccatcatgag
     1921 tgaaggccgt gtgatccaat ttgatacacc agaaaatatc ttacgtcatc ctgcaaacga
     1981 tttcgtagaa gagttgattg gagaagatcg cctactacaa gccaagccaa atgcgacaag
     2041 agtcggagaa gttatgctaa atacagcaat cacgattaca ccggaaaaat cattacaaga
     2101 agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg ataattcgaa
     2161 tgtattaaaa ggatttatcg atgtagaaac gttgaataaa aagcgaggaa aagtatccag
     2221 tgtcggcgac atattaaata aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga
     2281 taccttgcaa cgcattttaa aacgcggact gaaatacgtg ccggtcgttg atgaacagaa
     2341 caaagtcgta ggtatcctga ctcgtgcatc attagtggat atcgtctacg atgtgatttg
     2401 gggagaagat cccgcaacta ctgatgttgc agagtcagat actcctgaag aatctaagga
     2461 ggtctgacct atgcagcagt tttttcatca gcacggttca gaaattctct caaaaggttt
     2521 tgaacatctt tatatttcgg gacttgcttt gatcattggt atcatcattg ctgtaccgct
     2581 tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt gtgattggct taaccagtgc
     2641 cttgcagaca atcccttctc ttgctttatt ggcattgatg atcccgctat ttggcgtagg
     2701 taaattacct gcgattatcg cactctttat ttattcatta ttgccgattt tacgtaatac
     2761 gtacattggg atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag gcatggggat
     2821 gactgagttc caatccattt tttccgttga attgcccatt gctatgccaa cgatcatggc
     2881 tggtattcgt ttagctgccg tttacgtgat tgcatgggct acacttgcct cttatatcgg
     2941 cgctggtggt ttaggcgatt tgatttttag tggactgaat aattaccaac cagatctcat
     3001 tttggcaggt acgatccctg taacgatttt agcactattg gctgattggc ttctaggtct
     3061 cttggaacac cggctgacac cgatcgcact tagagaggag aatgaagaat gaaacgtata
     3121 ctaaaaatga ccatggttct tttctctctt cttatacttg cgagttgttc tttccctggt
     3181 cttgccagca atacagatga agacaccatt tcgatcacag gtggaatcac ttcagaagca
     3241 cagatcttag caagcctagt tgccggaatg gttgaacatt atacagataa aaataccgca
     3301 atcatcaaca atctggctac cacaactatc aaccatcaag cgatgatgaa cggagatgct
     3361 tcaatttcag cggctcgcta cacaggaaca gatttaacga ctacattgaa ccttcctcct
     3421 gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa aagatacgat
     3481 caaacgtggt tcccgtccta tggttttgaa aatacgtatg tctttcttgt tcgaaaagac
     3541 accgcacaaa aataccattt atctaaagtc agcgatctga aaaatgtagc ggatgaatta
     3601 gtagctggtg tagatacttc ttggatcaat cgtaaaggag acggttatga cggcttccaa
     3661 gaaacttatg gcttctcctt caattcgata ttacctatgc agattggact tgtttatgac
     3721 gcagtcgaag caggaaaaat ggatattgtt cttggctact ctacggatgg acggatcgcc
     3781 agttatgatc tagtcatgtt ggaagatgat cgtcaattct tcccaccata tgatgctgct
     3841 ccagttgtaa acaacaaaat attagctgat acgcctcatt taaaagaagc cttaaataag
     3901 ttaggtcata ccatcagtac ggaaaaaatg caggaattga actacgaagc agataataat
     3961 ctagtggaac catcagtagt agcacaacgt ttcttacaag aaaaccacta ttttgaaggg
     4021 aagtgaagca attggataaa atgaatgtct ttcaacaatt tttctattat ttccaggaaa
     4081 atggctccta tatctttgca caatttatcc gtcacttcct gatttctatc tatggtgtcc
     4141 tctttgcagc agttgtcggg attccggttg ggatcatgat ttctaggaga cgtaaattag
     4201 caaactgggt gattcgtttg gcgaatatca tccagacgat cccttctcta gccatgatct
     4261 ccattttaat cattggatta ggcctgggag tcaatgtcgt gattgttact gtatttttgt
     4321 attccctctt accgatcatc aagaatacgt ataccgggat gatccaagta gacaaaaaca
     4381 tccttgatgt cggcaaaggc atggggatga ctgctagaca acgactattc atggtagagt
     4441 taccactctc tgtttctgtt atcatggcag gcataagaaa cgcgttagtt gttgccatcg
     4501 ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct tggagatatc attatccgag
     4561 gaaccaatgc aacagatggc acatcgatta ttttagcagg tgcattgcca actgcattaa
     4621 tggtgattgt cacggactgg cttttaggta ttttggaaag acggctagat ccagccagcc
     4681 gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca
     4741 aaggcttttt tagttctgtc attttttaag gcaataatcc actctgattc ttccctcctt
     4801 atgggcaaag agttgctttt cttagctatt tatccattcc gtatactcca acttcatact
     4861 gccaacgggt tcatctctgc atccaccgac taagtttttt tcttatccgc taaggcattt
     4921 tgccaatcca ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc
     4981 gtctgcttgt tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta
     5041 agccttttcc atcagtcctc gattgttcga tgccttcagc tacatcatca aaactggtga
     5101 ctgccgtatc ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct
     5161 tggctaaaaa tatatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa
     5221 accggtacat gattcttatg gactgcatcc gtcgcaacta gacttggcgg tacgatgatc
     5281 cttcaccaga agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac
     5341 attggcgtaa aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc
     5401 cgaaagcgcc acgactttga cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc
     5461 tttggatacc cgctctgcta aagaaacgac actggtattg tatttcgtat ctgtatcttt
     5521 tccaaaagac catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc
     5581 cattgctttt ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt
     5641 cgcctcactt tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc
     5701 aattttactg attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca
     5761 ttccattcct aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag
     5821 aaatcatgca ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg
     5881 ggagactctt tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc
     5941 caggcagaat aaatttccac aactttattt tcctcatcaa ctttgtaaac cactcgatgc
     6001 tgtctattca gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ctcaaaagac
     6061 tgcgtgggca tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt
     6121 aaattcgatt gcttgatttt tcttaaatcc actgtcgcag agtttttgat tgcgactgta
     6181 tagttactca tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ccactgtcgt
     6241 ctttttcgcg gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt
     6301 gaatcgctct ccagtcttct agcccaatca atacagcatt gtttccacta cgatcactaa
     6361 taatctcgat ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac
     6421 gagctgtgct aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa
     6481 ttataacgta cttgattaag tacgtcaaaa aatacaataa agcacttaaa ttcagcaaac
     6541 ttttcctctt ttttacatag agaaaaagac ccctttttag ggaggatctt tgtccacgct
     6601 cacttcttac tcctttacga ctagcggtag ccaaacgtaa agtaattggt aacaacgtat
     6661 catctttagc aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac caatacacga
     6721 aggttcctcg ttgcagtgtg acttgattga tatcttgaat aacctgagga tacccacgaa
     6781 agatttgtaa cggttattca aaactgattt tggccgtcaa gtcttctact gcctcaaaaa
     6841 tgattgcctc tttcgtctcc tgccattcat gttcactatc gatttgatac ttgcttagac
     6901 cgtcgggttt gtgaatcacc aattaaaaat ggcactgaat tatggtaaca aaaaatggtc
     6961 acttgacgag gctgagattt ctacttatga agacacatgg cacttaagag tcgaggtaaa
     7021 ggaagaagct gaaaatatag gaggctgtac attatttggc aaaggattcg gaaattatga
     7081 tcaagacatc aaaatcaaaa tgttttacag ctaaatagga agaataataa catttcatac
     7141 gctgttccca tctttaatgg cttatagagg gcacctttag ctgggaataa gacaaaacag
     7201 accctctagc ttcaattaag agctagaggg tctgtttttg attttaaaac ttggtttatt
     7261 tcttcaaatg gtagctatag gtgctgacaa cgatattttc acgccaagac aatcgcaatc
     7321 ttaaccgcaa acttgtttgg ttatggagaa tattctgcca gcgtctgttt ggtacaaatt
     7381 gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc atttttactt acgatatcta
     7441 cataacggat aattggattt tcaatcgagc gataagaaga gtggacgatt gaaaaacgga
     7501 catctgggaa atgcttttta aattctgctt cgatttcttt ttctttttcg acatcttcgt
     7561 ctagcgaaac atgcatcgca acgacataat caccgatcga acgtgcgtaa ttcaacgcac
     7621 cgatatttac ccgtgtcaca ttcccgacta atacaagaac ggtattcccg tcataatcat
     7681 gtagttttgc atcattttct aaacgaagct gttctgctac ttttttataa tgatcatgga
     7741 tcttataaaa aatgaacata acgattggca taatgatgaa gaaaggccaa atgtccccta
     7801 accgatagac gaacaaaatc gcaatgattg catatgagat gaacgcacct gtaatattgg
     7861 caatcgactt gcttaaccat ctttttcctt cttttttcca tttgacgacc atccctgttt
     7921 gagataaagc aaatggaatg aacacgccga tagagtataa cggaatcaag cgttctgttg
     7981 agccatggaa aataaacagc aatacgatcg aacctagcgc tagtgtgata atcccattgg
     8041 aatagcctag tcgatcccca cgatcttgat acatgtgagg catgaattta tcttttgcca
     8101 gattgtatgc aagaactgga aaagcagaaa aaccggtgtt agcagcaact gctaaaatca
     8161 gcgctgtagc aaattgaagg acgtaataca aaataccatg tccaaagaca gctttaccaa
     8221 tttgagacaa aacagtgact tctttttcag gaacgatccc ataccaatag ttgataaatg
     8281 tgatccccac gaagaagaaa cctaagatca atgccatcat cgtcaatgta gcagcggcat
     8341 tttttgctct tggcttctta aagaatggta cagcattact aatcgcctcc accccagtta
     8401 aagaggaaga accagaagaa aatgctcgta aaatcaaagc gatagaaaca cccggcacag
     8461 ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc tgtcacgata ttatataaac
     8521 caacaacgat cagtaacgag atcacaagga taaatgtgta caccggaaat aataggaacg
     8581 atgcagattc cctcagccca cgaagattca gcatcatcag tagcaaaacg attgtcacag
     8641 aaatagcgac ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct tcagcacctg
     8701 ctgagacaga tactgctact gtcaacatgt agtcgatcaa taaagagcca ccggaaatca
     8761 agccagcatt ctttccaaga ttctcactgc tgacgacata tgctcccccg ccatgaggat
     8821 aagcatggat gatttgacga tatgatagcg ttaatgaaat caataaaata ataacaaaag
     8881 cggcaatggg tagcgaatac cagatggctg cagcagacaa agcgactaaa acgacaacga
     8941 tttgttccgt accatatgca atagatgata acgcatcaga cgacaataat gccaaagcag
     9001 caaatcgggt taatttgtgc tcatcatttt cagcagattt taaaggctta cctactaaca
     9061 acctttttaa gtaatccaat ttctaaactc ctttacacac aattttactt caagttttat
     9121 agtcgaaaat ttatagtaaa caagatgtta tagtacgctc actgacctta aaagtcaata
     9181 aactttaaga aaataattgg aaaaaaacag aaaaatatca agcgcttttt caaacgcttt
     9241 aatacagcaa aaatctaaca tttttttatg ggctttttct ttgaattcta taataaaagg
     9301 atagaacttg acttagaaaa tcaagttcta tcctttttga tatcttaact tttgaaagag
     9361 agatgtaagg ttgctttcta cccatcattc ttactactcg aagcatagcg cagttgtcac
     9421 aacctcttga taaacggtgt ttaaaagctt gcatctgcgg taataagccc aaccaaatta
     9481 aaaacataag aagctgtttt tatttggtcg ttcttattac ttgatgcaga acagcctttt
     9541 cacaacctct tggtacacgg tgttcaatca gctgaccctc ggcattagct caaaaaacgg
     9601 caaaatatga ggaactattt ttccattttt ttctctaata ctcaggtcaa aacgctgatt
     9661 tcacaacctc tttttttaat gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt
     9721 cattttttct tcgatgaacg gatagattgc cgttcctacg attccaaaga caacaaacag
     9781 accgatcatg atccagattg ctttcgtagc atttggccag taaatgcctc ctgcagattc
     9841 ccgtaacaag tttaccgcat gagtaaatgg caagaacgga ttgatcattt ggaagaattt
     9901 ccctgagact tggatcggat agttcccgcc tccgccagag atcgacagta ctaagatgat
     9961 aatagcgatc cctttaccga cattatcgaa taatgcagcc aagacataga cgatcatcat
    10021 gaacgctaag gcgatcaaga cagcaaatga cacgctgtag accggatttt ggacatctac
    10081 tcctagtaag aaaatattcc ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa
    10141 aaatgtcagc atacgagcaa cgaattgttc tcgtttcgta aaattgtttc ggtctttctt
    10201 tcctaaatag tattctgtcg tagcaacact tgaaagaaga acagcgccga cccacagaca
    10261 tagtgcagta taaaacgggg tgctagctga accgttattt gcgattggat aaagcgcatt
    10321 ggtttgtagc tcaacaggtt gtgtaaagaa gtcactttct tttgttgcat ctagtttcaa
    10381 taatttgatg acttcgccta gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc
    10441 tttttggata ccagctttga tatttggcca gtcattgttg atcaagtccg cagcagcgtt
    10501 cacagctgtt tcgacttctg gcattttttc atttactgtt gtcaatgtac cggtgatttc
    10561 attttttagt gctggccaat cattttggat aaaatcagcg gccaatccta atttttgctg
    10621 aaggatcggt aattcattgt tgtataggtc tgccccttca ttgatgccat tgacgatggt
    10681 atccatattt ccattcagca ttgtattggc atcatggatt tcttgtccaa ttgcaggcat
    10741 ttctttttga tatttttcta aaattgccac tgcattcgtt acagttgctt tcgttgaact
    10801 gagaagcgaa tcaaagtcga tctggtccgc tttgcttagc gccccttgcg cagtcccaat
    10861 cgtatcgatg acttggtcta atagagaagt gacagtggaa ctgatttgac ctgtattgat
    10921 accagcaatg atgccattga tattattaac aacggcatca atttgatcta gcaattcatc
    10981 aatagccttc gtgtcttcat tttgaacagc tgtgttcaaa gcagataggc ggttttgcag
    11041 attgcttagc aagggtttta attgatttag ctgatcaata attggctgca atttcgtatt
    11101 tccagcactt gtttgcaatt tagtcaggaa ctcgaccagt ttatcgattg cattttgttg
    11161 ttgagatatt ccgtcaataa agccgttcaa tacattgctg atgtttttct tttcatcatc
    11221 agtcagctta ccatcttcaa tagctgttct tacagtatct attaacgaac ctgtcgttgt
    11281 tgccacttgt ccaattgatt tcaatgtaac atctacactg tttgtgatgc ttggtatcgc
    11341 atctttcagc tgagaggcac ctgattttgt ttggtttgct agatcacttg cctgattttc
    11401 taaatttttc acatctggca agatatcctg tacttgttgg atgatcgtca agccttgttt
    11461 tgcttcatcg attccttggt tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc
    11521 tgctagttgt ttaccggcat tttggatctc tgggattttt tcttgcaaat caaggatgat
    11581 ttttgcttga tctttcaact caggcatttt gtcattcaat gcaacgactt tttcacccat
    11641 ctcatctact ttagggatat aatccacgaa ttcatttgct ttattcagtt tttccttgat
    11701 ttcaggtagt tgactttgta attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa
    11761 attttcatct gttgataaaa tcatatcttt gactttattg atacttacta ggttcgtatc
    11821 gatgtcgtaa cctatctcat tgaatacttt caataaagtc gaactggctg ttttgataaa
    11881 ttcatttgtg atttgcgatt ggatcgaact tgctccttta tcggtgattt tcggtgcaat
    11941 cgcattgatt ttttcattta ccgtatattc gatttttggt ttcttgatat caccactagt
    12001 aaaactcagc agatcttctg agaaatcctt tggcaagtaa atacctgcgt aatactttcc
    12061 ggaacggact ccgtcttcta attgttcttt tgaatcaacg aattgccatc ctaattgttt
    12121 atttttatga agcgattcga tgacttgctc cccaatggcc acttctttac cttgaaactc
    12181 tgctggcttg tctgcactgt aaacagcaat tggcagctct ccagtatttc catatggatc
    12241 ccataatgct ttgatgttaa accacgcata tagcgaaggg atgatcatga tcgcgatgat
    12301 cagaagtgtt gcaatcgggt ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag
    12361 cgtatttttg attgactgca tagttctccc ccttcttctt ttttcagctg aattacttta
    12421 ccatgtattt catacttttt tcaatcggct tttttcaaaa aagtcgaatt tttggacata
    12481 tcacggcaat tgtctcattg taatctctta catattgaga aataattaag gattacagac
    12541 tttactttat catgaaataa aagggaaaga taattttatg ccaaagtttt tatc
//
LOCUS       DACEMS010000084        12377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEMS010000084 DACEMS010000000
VERSION     DACEMS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.085"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW735_001983"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW735_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1044976.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW735_001984"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW735_001984"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1044977.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IW735_001985"
     CDS             complement(2169..2906)
                     /locus_tag="IW735_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1044978.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IW735_001986"
     CDS             complement(2997..3524)
                     /locus_tag="IW735_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1044979.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IW735_001987"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IW735_001987"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1044980.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IW735_001988"
     CDS             complement(4401..4886)
                     /locus_tag="IW735_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1044981.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IW735_001989"
     CDS             complement(5039..5236)
                     /locus_tag="IW735_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1044982.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW735_001990"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW735_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1044983.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW735_001991"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW735_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1044984.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IW735_001992"
     CDS             9211..9423
                     /locus_tag="IW735_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1044985.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IW735_001993"
     CDS             9425..11104
                     /locus_tag="IW735_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1044986.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IW735_001994"
     CDS             complement(11358..11558)
                     /locus_tag="IW735_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1044987.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IW735_001995"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IW735_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IW735_001996"
     tRNA            complement(12249..12322)
                     /locus_tag="IW735_001996"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
BASE COUNT         3616 a         2668 c         2148 g         3945 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaactg agctaagagt acaggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaaggtgtg gcaacaa
//
LOCUS       DACEMS010000085        12171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEMS010000085 DACEMS010000000
VERSION     DACEMS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.087"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            592..1920
                     /locus_tag="IW735_001997"
     CDS             592..1920
                     /locus_tag="IW735_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1044988.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="IW735_001998"
     CDS             2122..2955
                     /locus_tag="IW735_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1044989.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="IW735_001999"
     CDS             2971..3708
                     /locus_tag="IW735_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1044990.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="IW735_002000"
     CDS             complement(3863..4831)
                     /locus_tag="IW735_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1044991.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="IW735_002001"
     CDS             5063..5896
                     /locus_tag="IW735_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1044992.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="IW735_002002"
     CDS             5930..6769
                     /locus_tag="IW735_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1044993.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="IW735_002003"
     CDS             6795..7355
                     /locus_tag="IW735_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1044994.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="IW735_002004"
     CDS             7554..9275
                     /locus_tag="IW735_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1044995.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="IW735_002005"
     CDS             9440..10582
                     /locus_tag="IW735_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1044996.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="IW735_002006"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="IW735_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1044997.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4149 a         2042 c         2526 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cagaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DACEMS010000086        11711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEMS010000086 DACEMS010000000
VERSION     DACEMS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.088"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            104..967
                     /locus_tag="IW735_002007"
     CDS             104..967
                     /locus_tag="IW735_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1044998.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1096..2718)
                     /locus_tag="IW735_002008"
     CDS             complement(1096..2718)
                     /locus_tag="IW735_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1044999.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2967..3488
                     /locus_tag="IW735_002009"
     CDS             2967..3488
                     /locus_tag="IW735_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045000.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3674..5404)
                     /gene="spxB"
                     /locus_tag="IW735_002010"
     CDS             complement(3674..5404)
                     /gene="spxB"
                     /locus_tag="IW735_002010"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1045001.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5569..6387
                     /locus_tag="IW735_002011"
     CDS             5569..6387
                     /locus_tag="IW735_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1045002.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6490..6687)
                     /locus_tag="IW735_002012"
     CDS             complement(6490..6687)
                     /locus_tag="IW735_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045003.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6856..7434
                     /locus_tag="IW735_002013"
     CDS             6856..7434
                     /locus_tag="IW735_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1045004.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8479..8982)
                     /locus_tag="IW735_002014"
     CDS             complement(8479..8982)
                     /locus_tag="IW735_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1045005.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9340..10365
                     /locus_tag="IW735_002015"
     CDS             9340..10365
                     /locus_tag="IW735_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1045006.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10688..11566
                     /locus_tag="IW735_002016"
     CDS             10688..11566
                     /locus_tag="IW735_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045007.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3642 a         1996 c         2280 g         3793 t
ORIGIN      
        1 tgattagaac ttgcgaaaat aaaccggtta aagtgtgtct agcgcaagag atttgctata
       61 atagacgata agggtaaaat gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac
      121 agattcttgt tgtgatctgc cataccatgt attagcaaaa gaaaaagttg attttttatc
      181 aatgaatctc caattgaatg atcaagagtt agtagatgac ctcggaaaga cattcaatta
      241 tgaccgctac atgaatcagc tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt
      301 tggccgatat ttagaatttt tccgttctta tgcggaaaaa cagattcctg ttttatatgt
      361 ggctttttcc agtggattaa gcggctctta cagtagtgcg cttcaagcgg ttgaattgct
      421 gaaggaagaa tatgaagacc ctgagatcta tgtttttgat acaaaagctg caagtattgg
      481 ggaagggctt ttagtattgg aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga
      541 aaccctagag tggctggaaa gtaattattt gaaagtccat tcatgggtaa cagtagacga
      601 tctgaaatat ctagaacgag gcggtaggat ttccaaagca agtgctactc ttggcagtct
      661 attgagtgta aagcctatta ttacggtgga taaagaagga aggctgacga atgttgacaa
      721 agttcgagga agaaacaagt cgatccagac aatcgtcaat gagacggtaa aaacaatcgt
      781 tgcaccactt gatcaaatcg tttatgtggc ttacgctggg gatctagaga gcgcggagaa
      841 ggcaaaacgg cttttggaag agcagataaa gatgaaagat gtgaagcttt atcccttagg
      901 accaacgatc gccagtcaca caggctatgg ctgtatcgcg attttttcga tgggaatcag
      961 tcgataaaag cagtaactca ttctgtaaaa aagcccagaa attgattgta acaaaaacag
     1021 cgactcttaa aagatagatg agcatcctat ctcttagagg tcgctgtttt tttatttttt
     1081 aagctaaata ggccattatg cccataactc gttcaattgt ttttgaatat ctgcattttc
     1141 caaaaattca tcatatgtcg tatcagctcg atcgatcaca cctttatccg aaacggcaat
     1201 gatgcggttt gccaatgttt ggataaattg atggtcatgt gacgcaaata agattgagcc
     1261 agtaaatgcc atcaagccgt cattcaatgc tgtgattgat tctaagtcta aatggttcgt
     1321 tggatcatct aatacaagta cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg
     1381 gactttctct ccaccagata aaacattcac tggtttcaat acttcttcac cagaaaacaa
     1441 cattcttcct aagaagcttc ttaagaaggt gttatcatct tcttctttac ttgcatattg
     1501 acgcaaccag tctaaaatcg ttagatcagt atcgaattct ttcgttgtat cctttggtaa
     1561 ataagaatta cttgttgtca caccccaacg tactgttcca gtatctggcg taatttcacc
     1621 catgataact ttgaatagcg ttgttgttgt tatatcattt tctgcgacga aagcgacctt
     1681 gtcttctttg ctcaaattaa aagatatatc atctaaaatt ttctttccat caatcgttac
     1741 acttacattt tctacctgca ataagtcgtt tccaatctca cgttcaggtt tgaatccaac
     1801 gaatggataa cgtctagaag aaggttggat atcgtctaaa gtaatttttt caagcatttt
     1861 tttacgtgaa gttgcttgtt ttgattttga tgcattcgca ctaaagcggg caataaattc
     1921 ttggagttct ttgatttgtt cttctttttt ggcatttgcg tttgcttgaa gtttagccgc
     1981 tagttgacta gattccaacc aaaaatcata atttcctaca taaagtttga ttttaccaaa
     2041 atctagatcc gccatatgtg tacatacttt gttcaaaaag tgacggtcat gggatacaac
     2101 gatcacagta ttttcaaaat tgatcaaaaa ttcttccagc caattgatcg attggatatc
     2161 cagaccgttt gttggttcgt ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc
     2221 taataacact tttacctttt gtccaccagt taattcactc atttgctgat ggtgcaattc
     2281 ttcagggatg ttcagacctt gcagtaaaac agctgcttct ggttcagctt cccatccatt
     2341 aagctcagca aattctcctt ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc
     2401 ctctttcatg taaatcgcat ctttttcttt cagcacttca tacagacgct tgtgtcccat
     2461 gatgactgtt tcaagtacag tatagtcttc atagtcgtaa tggttctgtt tcaatacggc
     2521 catccgttca tctggtccca tagtaacgga acctgtggaa ggctgcagat cccctgataa
     2581 cactttaagg aacgttgatt tacctgcacc atttgcacca atcaagccat agcagtttcc
     2641 gggggtaaat ttaatattca catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac
     2701 atcatttaca gtaatcaatt tttttcctca cttttatcat tattagtttt gacaagtctt
     2761 atagaattat ataggggaaa ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca
     2821 cgtgaaacag aatgaaaaaa ataaaaataa aagcggagcg aaaatagcta ctataggatt
     2881 tattagtttg caacagcata atttttgacg ttatttataa gagggagtac tataaaactg
     2941 taaaagaaat gaaagaagga ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat
     3001 tgattttggg aattttattt gtacttgtct cattgttatc atttcaagac cctgtcggaa
     3061 atttagttgc aattgtagta gtctttgcga tttttgcctt tattaaaggg atatttgaac
     3121 tgtttgtacg gaacagatta aaagagctaa caggatataa aggaaagatg cccttaataa
     3181 tcgggatcat cgatattcta gttggcgtct ttttcttatt caatattggt gcaggagtcg
     3241 cagcattgcc ttttgttttt gcagtatggt ttattgctga ttcagtttta gcgttattta
     3301 ctgctgactt ggcaagagga gtaagtgaag gatattattg gtttacgatc atcgtcaata
     3361 ttttaggaat tttattagga attttcttat tgtttaatcc aatttcttct gcgttgacat
     3421 taagcttttt agttggattc tattttatgt tatttggtat cacacatatc gtctatgcat
     3481 ttagataagt aaaatcaaac aaataaaaaa gccggcttcc gattacagtt ttactgttgt
     3541 aattggaagc cggctttttt atcatggaca aatatgcacg aggtgatttt taggtgtcga
     3601 gacaaattta tgcaaaaatg tgagaagcta tatacacaaa ttttatctta atactcgaag
     3661 catagcgcag ttgtcacaac ctcttttcat ataattcatt caatactggc aaatcatgga
     3721 cttcatattt ttctttgaat gcttggattt cttcttcaga atattttctt ggatctaaat
     3781 gcagatcttc tactggcaat gggcgcttgt tattgatctt cacatcgatt actacaggac
     3841 gatctgattt tgcagctgca tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa
     3901 agctatttgc cccgagcgct tctccaactt taccgaaatc tgcatcttct aaaaatacac
     3961 caaatttctt ttgttccgta tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg
     4021 aaaagacaac attaataatt ggaagctgat atttgacttg tgtaatgata tcttgcatga
     4081 ccatagcaaa cccgccatca ccacttaagg taaatacttg tttttctggg tagcttagtt
     4141 gtgcggcaat tcctcctggc acaccattac ccatagttgc gaaccatcca gatgtcgtat
     4201 gtttttgttt accgttcata ttcaataaac gaatagaatg gatagtcgtg ttaccgacat
     4261 ctgtgacgaa aatcgcatcg tcttcagcaa tacgattgat ttctttatag acagcctctg
     4321 gacgaatcgg ttcttcctct cgatcttcaa aagatttcaa ccaatttacc catttttctt
     4381 tattttcttg atttgctagg taccagctat cagcagggcg agcatcccct aactcgacaa
     4441 gtctttgcaa agctgtgttt gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc
     4501 caaattttga cgcatcgata tcgatttgga taaattcagc agttggatta aaaaatgcac
     4561 gtccaaatgg gaagtcgcta ccgacaaata agatcagatc ggcttctgcc aatgcttcat
     4621 ttccgggttt agtagcgaca cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa
     4681 taattccttt tgctagaacg gaagcgacaa caggaactga aaaatgttct gagaattctt
     4741 tgatttgcgg aaatccgttt cgcgttcctt gaccaacata aagaactggt ttttttgctt
     4801 tttcaagata aggcaaagca gctagcaaat ctttttcttc tggtaatatc acacttgttt
     4861 tgtgagtgtg agcggtggag aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag
     4921 ggatagtcac gatagccacg cctttatgct cataggcggc tttaatggct tcatctacta
     4981 catgcggaag actttgtggt gtcatgactg tacgattgta tacgctaaca tctgcgaaaa
     5041 taggattttc atttagttcc tggaaagagt tatagttcat ggatgtagaa gcaacttgtc
     5101 ctaagaacgc tactagaggc acatggtcca tctgtgcatc atacaaacca ttgatcaaat
     5161 gtgtcgcacc aggtcctgca gagccgaaga cagcgccgac tttccccgtt aattttgcat
     5221 ctgcagcggc tgcgagtgcg ccgacttctt catgacgaac ttgaatgtag ttgatttcgg
     5281 ttttttcttg atatagcgca tccataattg aattgaaaga tcctccggga atgccgtaaa
     5341 tatggtcgat accccagctt tcaagaactt ttaccattgc gattcctgca ttgatttttg
     5401 acatggtgtt tcactccttt agcaatgatg atacataaat tttttgtatc tagagtatag
     5461 aactttttat cgaaaatccc aactaattcg tcttttcaca aaaatgtagt catgtttttg
     5521 ggaatatgct agagtagtac atagatagat tcaagagagg ggaatggcat ggttgaatgg
     5581 ctattgagac ttgaaccttg gcagcaagca cttgctggga cagcttttac ttattttatg
     5641 acagctcttg gtgccgggtt ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac
     5701 ttgatgttag gatttgcttc tggtgtcatg attgctgcaa gtttttggtc tttgttagat
     5761 ccggctatta caaaagcaga agaaaatgga gatattgctt ggctagttgt aagtattggg
     5821 tttggtttag gcggtgtgtt tttatatatg gcagataaaa cactgcccca tatgcatttt
     5881 ggaccacaac atgaagcaga aggattgcct acccatttaa aacggacgat cttgcttgtt
     5941 ttttctatta cattgcataa cattcctgaa ggtttagccg tgggggtagc attcggtgca
     6001 gcagcaactg cggataatcc gacagccgct atattagccg ctgtttcggt ggcgctaggt
     6061 attggtatcc aaaacttccc agaaggagca gctgtatcaa tcccgttgcg acaagaaggt
     6121 ctgagtcgta aaaaggcctt tgtttacgga caagcttctg gaatcgttga accaattgct
     6181 ggagtgattg gagcgctact agtaacgaaa gtggagctgc tgcttcctta tgcacttgct
     6241 tttgccgcag gagcaatgat ttatgtcgtg gtggaagaat tgatccctga agcacagcag
     6301 acattgtcca gcaaacgtca ttttgcagtt tttggcgtca tgagtggatt tatcattatg
     6361 atgattttgg atgtggcatt aggataaata aaaaaaatat atatgatata tcaaataggg
     6421 ccgcgaactt atatcgcgac cctatttttt tagtaagtta tatcataatg aagtgaatgg
     6481 tatcatacat taataatagt tttttctatg tgtatttcct ttgatccagc gaacaagcca
     6541 gatgataagt gcgattggga ttagaacttt gaccgcaaac cataaaagcc cgcttaggac
     6601 actgacaaaa agactgatga cgaagctagc tgcaattagc actaaaagaa ccatgaagat
     6661 atttaagaac atattttttt gacgcataca aaaccctcct cttgttcatt ctttactata
     6721 gaaaaagtat aacagggatt gtttaaaaac acctccaact gaagactgat ttttacttca
     6781 tctacttaaa tgatcaaaca attgcttttt ctctggttta tcatctaaaa tagagcggaa
     6841 aaggaggaga atatcatgcc taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg
     6901 attatcatga ctattttagt tttagaactt catgaaccta acggagacac gtgggaggcg
     6961 ctcgctgcat taggttaccg attttttatc tatattgtca gttttatcat tttagcgatt
     7021 tattggaaca atcatcatca tttatttcaa gttgtacata agataaatgg acgggtctta
     7081 tgggcgaaca gtttctttgt ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc
     7141 agtgagtaca tcaatagcct ggctccagaa atgacttatg gttttgttac cttgaccgct
     7201 aatattgcgt attatatctt ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa
     7261 gcattgaagg cgatgttgaa taattacaag aaatcgtatc tatcgattgg tttgaatgtg
     7321 ttaggaattg tcttagggta tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg
     7381 attctagctg cttggatcat cccaagtcgt accatcgaat cacaatatcg gtaaaatgat
     7441 ccgtgtttaa aatttaagag aaaaatatag aaaaaataaa taaacgatca aatatccgag
     7501 tgatactagt attcaaagag ggatgcagat ggatcaaaaa agaattcatg taggactgat
     7561 tactagtttt gtcacgatgc ttatttattt ggatcaaggt catttgactt ggacagtagt
     7621 tggtgtcgta ggatttttga cattattcac agcaataaaa aaataaacat ctttatgtaa
     7681 tctgatgttc tatctattta agaaaagcat gtttacagca gtatgattct gttgtaaacg
     7741 tgtttttatt tgtggaaaat ttaattttta gaaaagtgta tcgaaaaata gatagttagt
     7801 ttttccacag tggataactt cgtaagagat aaaaataaaa aattagtcaa agaagggcgt
     7861 atagatttct tttgatagga gatatacaac agaaatgaat aaatattaac gtgttttcaa
     7921 acagtataaa acggtgtggt ttttggaata aaaaaataac atatctcatt ttacactttt
     7981 gttattttat acttaaaaaa taacgacaca gaaaataccg tgtctcagat ttctctacta
     8041 cttctaaaaa ctactgtttt tttcctttga aatgccttca aaaagtagga tcaggcagaa
     8101 tatactggca gaatcaagga gaagggagcc taaataagga gaaaaaccat agaaaatata
     8161 aagtaattac ttataaaaca agctttttat agaaaaataa tatacctgag tagggtataa
     8221 attaagacgg aattcctttc tagattgaga tattgactag gaagaatgtt aagattttaa
     8281 gcctttaagc taaaaaaaaa ccctttttgt taaataaggg gaatatagcc tgtcagtttc
     8341 aaactgcaag tcgtcctgct gcaataatat agtagattag agatgaaaat agattttttt
     8401 aaaccaatga ctgaataaaa agaccgtcat agatacacat tggaatgtgt ctattacggt
     8461 ctttttttac gaacgatttt attgaagacg gacagttcgg gtcaaaaata aaaaagctac
     8521 gatcagaaat aagaataaaa tgtatcccaa aataatggct agacggtcta acttatttgt
     8581 cgatagatgt ttgtttagaa gttgataggc ccataaacct atcagtcctc ctgttgtatt
     8641 agttatcaaa tcagtgatgt ccgctacgcc aataccgaat aggaattgaa tcaattctgc
     8701 caagaagctt acaaggaaaa taatccctaa ttttttagaa aaagaaagtc tcttgaaatt
     8761 gacattcaaa agcaccccaa aaggaaaaaa gacaatcaag ttataagtgg tctcacgtaa
     8821 taccgtcgta ttttcaaaag aaaaagtaga aaaagggacg agattgacat tgctgtaaga
     8881 ataagtgaga aattgtggaa gattaaatga tagcttgaat aaaatcaacc ataatagaaa
     8941 gaataaataa atccctaata aaaattttga tacataccgc atagacatct cctccattta
     9001 aaaagtatac catcgaaaac cgctgaaaaa tcataaaaaa gtatgaagtt taaggaccaa
     9061 cgtagttatc gtacgaaaat tagaagcttt ttttgttggg aatccggttt ctttaacaaa
     9121 gctaagatag cgagttgctt ggaaaagttg ctatcttttt tctgtcaaaa cgactcggaa
     9181 tgttcgtttt ttgttctaat ttctccaagt tgtttacttt ttgatactaa gtttatgaaa
     9241 aatcaagaaa tagttgttat actaggtaat gctgacaaac gggattggca tttcatcttg
     9301 gttcagcttt ttttccaatt actaggaagg agggatagat tgttcaagac ttattttcgt
     9361 ttcaataaaa tacaagataa tccttttcgt atcatgaatc tgatgctact tatctttatt
     9421 gtattatggt taggttacat aaatcatgat tttttgattt caagtttttc atcattaggg
     9481 atctttactt ttttctatta tcaaaacata ccgatgaaat atttgctgaa acgtatgttt
     9541 ctgatcggtt gcggactgtt ggttgctttc gttttgggga tcttatcgac ttatgtttta
     9601 tggcttgaac catttgccgt agctttggtt gctttttcca gcagattcgt gttgcgatta
     9661 ttccatattt ctaaaccagg tggcttgttt tttgcgatgc tttctgccat gggaacgagc
     9721 atgcagctgc caatcgctca attaccgatc gtctcacttt atttttttat gggtgtcgtt
     9781 tttgccctga ttgcggcagt gattacaaaa ttactggatt cacggcctga gcagacaatt
     9841 gaaaaagcaa ctttaaaaga aagatttcac gaagagcctt tagtgatcat tgattctgtt
     9901 ttctacagcg ctgccttatt tctttccgtt tatgtcagtc acggcttgaa tctgcataat
     9961 ccttattggt tgaccttgtc atgtgcatcg attttgctag cagaaaactt ggatgcgatg
    10021 aagcatcgac aagtccaata tctgattgga tcgatgggtg ggttatgtgt gtcggcattc
    10081 ttgtcatttg tgccgtttac gcagttgcag accatttttc tgatcacttt tctatatgga
    10141 atcgcacaat ttctagttgc tagaaattat gcagttgcta atatttttct taatccgatg
    10201 gcgttgatgc tatctacact tattcgtgga gcatacttga tttctttgat cgaatatcgt
    10261 tttttaggga tcgtcatagg aagtttcatc ggtttaggtg ttgcgtgggt aatgacggtt
    10321 ggtttgcaac attatttaac ggttgttcgt caaaaattag aatagaagga caaatatatg
    10381 tgccaaaaga ggttatgaca agatttttgt cacaacctct aattcaaggt gttcaaaagc
    10441 cagattctca gcattaagtc aaattcacac aaaaatgtgg aaaggaactt tcaaaaaaat
    10501 tttatattta ttctatcaaa aaattttgtt tatgatggta actgaataaa ctttcctgag
    10561 atggtcaatg ctttccttga aagtcttaca gatattcttt cttcatagaa agtttaagtt
    10621 ttatagttag tgtacaataa aagtatgttt ataccatatg gtgtccggat aatcagtcat
    10681 ttaaacaatg aggttcaaag atatgaaaag gggaaatttg atgaagaaaa taagcaatga
    10741 aatccaaatt ctggcagaaa ctttaactct tcccaaagtc gttatagagc aaatgattgt
    10801 acaagcaaaa aacatccttg atgaggtgtt tgagcaatat agtcatgaac taatggatcc
    10861 taataaagca aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc
    10921 agcattagct atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt
    10981 gaagattcca agaactatct atttggcaac aatgaatgct tttactagat ttattcatga
    11041 acatttccaa gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac
    11101 tagtggcttg atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg
    11161 gtacacggat ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc
    11221 agatttatct cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact
    11281 ttattttcct gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa
    11341 tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt
    11401 attatcagtt gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc
    11461 ccaaatacat gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct
    11521 tgcaggcgag cacattggct ctggcttagg tattcttact tcataagttt agtacttcga
    11581 aatagtttaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    11641 gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
    11701 gaaaagcaat t
//
LOCUS       DACEMS010000087        11451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEMS010000087 DACEMS010000000
VERSION     DACEMS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.089"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2566)
                     /locus_tag="IW735_002017"
     CDS             complement(<1..2566)
                     /locus_tag="IW735_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1045008.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLE"
     gene            complement(2579..3778)
                     /locus_tag="IW735_002018"
     CDS             complement(2579..3778)
                     /locus_tag="IW735_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1045009.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(3768..5363)
                     /locus_tag="IW735_002019"
     CDS             complement(3768..5363)
                     /locus_tag="IW735_002019"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1045010.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(5947..6654)
                     /locus_tag="IW735_002020"
     CDS             complement(5947..6654)
                     /locus_tag="IW735_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045011.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(6719..7555)
                     /locus_tag="IW735_002021"
     CDS             complement(6719..7555)
                     /locus_tag="IW735_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1045012.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(7573..8982)
                     /locus_tag="IW735_002022"
     CDS             complement(7573..8982)
                     /locus_tag="IW735_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1045013.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(8996..10819)
                     /locus_tag="IW735_002023"
     CDS             complement(8996..10819)
                     /locus_tag="IW735_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1045014.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         3342 a         2426 c         1883 g         3800 t
ORIGIN      
        1 cttccaaaag ttgaatttga ttgttcaaag ttggagattt caccatgtca tcattgtaat
       61 cgtcataagt cactaacgat tcataagcac gattcaattc ttgaaatgct ttgacgtact
      121 cgatacggat ttcaagtggc gtatgttcat caatggattc catggatgaa acctctttta
      181 acttaccata tgcttgtttg aatctttttt tggattcctg ataacttgga taaatcaaac
      241 ctgattcttc ttttgctttt gaatacagtt ttgtcgcatc ctcgacattc ttctccattg
      301 tcgctggctt acgaaacgtc acaatcaatc cttttgtctt atcaggatac gtacgattcg
      361 tacgtgaaaa agcttggatc aatcctgcat actctagatt acgatccaca aatagggttt
      421 gaatcgtagg ggcgtcaaaa cctgttaata gacgatccac tacaatcgct aaatcgattt
      481 gtttcccaaa ttgtttgaat tccgctcttt ttcgtgccaa acgattattg atgtctccgt
      541 tataacgctc aatgtctgct aatgaccagg aagtatcata atactgatta tattcctcaa
      601 taattttttg catttcttct tgttgggcac ttgaatctcg gctattttct tctaatgaat
      661 aggtaatagc gattcgtgga aaatcaggat cctccatcgt tcgtccttca cgaatgagct
      721 gattgggaaa ctcttttgtt aaccaatcag gctctttcgt catctctttg atggcccgat
      781 aatacttttt ggccatgtca atggaactcg tggttaagat tgcagatttc gttggcctgc
      841 catttcgaaa atcaaatttt gtataggcat tatctggacg gaacatttta tgaatgactt
      901 tttggatatg ttcatctgct tcaaaaacag ctgggtccaa ataactttct tttttgacag
      961 gttccatttg atcaatcata cgattgatct gttctgatga atactctgca tatgtttcta
     1021 cttcttttaa tttccgatag attttattct ctagtgaagt cggctccacg gtgttttcat
     1081 gctctacttg aaatcctaag acagagccat cttctagcgc attcttaatg gtataggtat
     1141 gcaagacctc tccatactga tcatgggttg tacgtgccaa ttgccctttg gcttgtttcc
     1201 gattctcagg aaaaatcggt gtacctgtaa agccaaacca agtggatttc ggaaacattt
     1261 ttttgatttc ttccatattt tcagcactta acgctcgatg acactcatct actacaaaca
     1321 cgatatgttg gcccattaat ttttggaagc gattggttcc ttttttctct tcttgtttct
     1381 ttgcatattt caacgccgca tctagttttt gacgggttgt aatgattaca acatttgcat
     1441 tggcatctgc caacagggtt tcacttaatt ctttggcact tcccgttcca acgatcaagc
     1501 tattcgcttt ggcgttcccg gaagaaatcc cagtattaaa ttccgaagca aattttgtaa
     1561 actctgtggt ggtttgatta tctaaatctt tgcggtccac taacatgatc gttcgatcga
     1621 tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc tttcctgaac
     1681 cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac aaggcttgaa
     1741 tcgcatgtac ttgataagga tgtaacacca tgagggtttt attatcttga tcttcactga
     1801 cgattgtata atccgcaatc aaacgatgag catccgggat atttaacact tgtttacaaa
     1861 actcatataa gttttctact ttttcattgt ctttcgttcg ccaactaaat aaaaacttag
     1921 ggtgcaagtc tttcggtaag gcatttgcaa aataccgagt ggtttgttca ttggaaacca
     1981 caaacaactg taaggtcgaa aagatattgt tccgaaacat accttcttct gcatattttt
     2041 tgatttggtt aaatgcctga taaacaccat cttttgcagt tacttgtttc agctcgattt
     2101 ggacaatcgg taatccattg attaataagg tgacgtcaaa acgtcgatcc cgtgcttcta
     2161 tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt cctccaccaa
     2221 tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcgaatct tcccgttcaa
     2281 tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca aatggggttt
     2341 tcgttcgcag cagtaattcc gttttgatcg tttcaaattc tttatcggtc aaaggaaaat
     2401 cattcagttc tgcttgatta tttgagataa ttttttggcg taaattgacc caaaggtctt
     2461 cttctgattt tagatccgga cgatacgtcc actgattatg cccttctcca agtacacgaa
     2521 tcaactgttc ttcgacttct gcttcctcac ggtgactaat tttcattctt cttccctcct
     2581 agacaaacat cttttgtaag aagctttttt tcgtttcttt tagtaaatct aacttacgtt
     2641 gatgaagagc gatcgtatca tctagctgtt tgaagaaagt accgattttt tgttgttcat
     2701 cctttacagg aaccttaaca ctgatattct caatatcaga actgttataa gaaggcaatg
     2761 cccctacttg ggagtatctt ggcaaattta tagtttcaaa caatatttta ccaaaataga
     2821 tatcccaagt attattaaat aaataagcca tggtattgtt atctattaag aaagggcttg
     2881 tgaccaaacg ttttctatta agcattattg ctgcgccaac tttagcaaat ataatcgctg
     2941 gcacatcttt tattggcttc cattttttac gctcaatttg ctcattagat acataatgat
     3001 ttgcatttct catttcatac tcattaccaa tgttattcat gtcggaaact ttaaaaaatg
     3061 gagtaccttc agaaccacct tgctcggcat caggaaatcc aattcccgat tgagttttac
     3121 ctaattctcc caacttacgc tcttcccaat cttccgtaaa accaggaaac cggatttctg
     3181 gcacttttgc tccgttttca gggaacattt tttgtaagaa tccttttttc gtttccttca
     3241 gcaaatctaa cttacgttga tgaagagcga tagtgtcgtc gagttttttg aagaatatgc
     3301 cgattttttg ttgttctttt aaatcattgg gaatatagaa tactgtatct gaaacttttt
     3361 tccagtctga acgaggcatt tttgttccag atgtatcatt tgcagccttt tgatatctat
     3421 cagcttgaat aagagaataa ataaagtccg agtcgctctt cttagctcta aaaacccaga
     3481 aatccccaat cgctacgcct gtaaaagttg ccttgagcca attttttaaa tatggacgta
     3541 actttccata tagaatatca tttggtaaaa attcaatccc ttttctatta tcaaatttct
     3601 ggctcacatc tttattcagt ctaccttctc ctgccacgat atcttcaaat tcaacttttg
     3661 gaagtctact ggagtttgta cttttattaa gtctaacaac taaatcaatt aacttacgct
     3721 cttcccaatc ttccgtaaat cctgggaaac ggatttctgg tcgtgtatca ttgctcatgt
     3781 ttgaacacct ccagcgcacc ttgtaaccac atttcgtttt caggtgttgt ttgcagggaa
     3841 gagatagctt ctaaaagttc tttttctagc acttgctgtt cttgtcgtat cttcttgatg
     3901 tcatttccga catgaaccat atcaattggt tcttcttctt cgaaagtatc tacatagcgt
     3961 gggatattta agttatagtc attttctttg atttcatcaa aggttgccaa atgagcgtat
     4021 ttctcgacat cttttctttc agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat
     4081 agtttatttt gatttttccc tttaacaaat tcacgactag catcaatgaa taatacatct
     4141 cgagtttgac gattcttctt caacacaatc accgtcgttg gaatcgatgt cccaaagaac
     4201 aagttcgcag gcataccgat cacagcataa atactgccat cttctagtaa tttttgacgg
     4261 atgacccctt ctgcagcgcc acggaataaa actccgtgtg gtaagacgat ggccatggta
     4321 cctgtttcct tcaaatgata gaacccatga agaagaaacg caaaatctgc tttggatttt
     4381 ggcgctaatt tcccataacg attaaaccga gaatcatcta agaaagtcgt atctgctgac
     4441 caattagccg aataaggtgg gttcattact actgcatcaa atgtgtaagg ttcatctgtt
     4501 ggccagtcct tattcaacgt atccccattg cggagattca tttcttcggc gtcgacacca
     4561 tgtaaaatca agttcatttt tgctagattg tatgtcgttg tatttagttc ttgcccatga
     4621 tatttaacgt tatctgggtg tgttaagtag ttacgtacgt ttaacatcaa tgaacctgaa
     4681 cccatcgttg gatcaaaaac actaaagaat cgtcgttcct tttgatcgag cgttacgatt
     4741 tgcgccatca tatcagaaac catatgaggt gtgtaaaatt ctccggcttt cttccctgct
     4801 tctgaagcaa attgactgat cagaaactca tacgcatccc cgatcacgtc cccatcgtgt
     4861 tctaaaacat ctacatcatt caatttcttg atgacttcgg taatcgtcac atttcgttgt
     4921 tgctcatctg ttcctagttt ttttgattgg agatccacat catcaaataa cccattaaat
     4981 tgattgtagg tagatgccaa ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt
     5041 gcttgttttg cttgatccgc taagacgttg aaaagatact ctggtgcaat tgcatatccc
     5101 aaaatatcaa cgattgtggc aatcagatct tcttttgatt cttcatctga aagtaattca
     5161 cgatacaaca ttgtttgttt gcttactgtg tcatattcct ctaaggattc atctgctaaa
     5221 agaaccactt gttccaacaa tcgatcggat aaatatttat agaaaatcag tcccaataaa
     5281 taatttttat attctgacgc atccatttta ctacggagat tatctgccgc actaaataat
     5341 ttttgattta attctgcacc caaaaagata cactcctatc tcttatttaa agttgctata
     5401 taccctctta ttttagcata ccccctttta taaaaagcaa aaaaaaaaga aagaaactta
     5461 ttcttgctaa taaactacgc gaaaggagtc taacgttgtt tgatataact tagactgatc
     5521 gttaacttat tttttatatt tctacatagt tatctttaga accatttcag atacatatga
     5581 aacaattaca tatgattttt tcataaaaaa acagtactgt ctagtagtat aaaatataca
     5641 aatttcttaa aaattttttc actaaaatca ctacctgaaa cagtctaaaa cacttatata
     5701 tcaacgttta atgaaactat ctccgtttca tttcagatac aaataaatat agtcgggctc
     5761 caaatccgaa aaaaaatacc cccagtagct tatcctaagc tactggagat gtataagtac
     5821 ggtcatataa caacggggta gggtaaataa atgaaaaaga tggaagaatc attattcaat
     5881 aactttttta aatacttcgt tgtaatcagt tacctgtccg ttcttaaata ccacagaata
     5941 atctttttac ccattttcaa gtaaccgaag tggaatgtct ttaccaactt gctctgtcac
     6001 tgcattactg atacctgtta gattagctaa taaatctgat aaagattgag catctgtaga
     6061 aatctctgta tcagtattta tcgtgattcc tttatcttca tttacaagct tccaagtagg
     6121 tggtatagct gtgtatgttt cgatgaatga atcaattctt tcatctatcg tcttcccagt
     6181 atctacttct gtctcattac tcttagatga atcgattctt tcatccatcg tctgctccgt
     6241 aactgcttct gtctcattat tctcaccgga aactctcttc acacccaaac ttatatattg
     6301 tccatcttta gtgtgcatag ttaaaattcc cacgctttta ctaataaatt cactataacc
     6361 agttctttca atagagactt caatgtattt cacactatca tactcaaaag aaaaatagac
     6421 atgtaaaagc attttgttgt ttacatctac ataataatcc tttgactcta cttgagacct
     6481 tcctatacgg aatacagctt tactgtctga tatctctata ccagcatcct tattattaat
     6541 ctgccaatct ccaagtagct cttctggcaa gctagtagtt tcttgtgaac actttaccac
     6601 ttcactggta gaggtcgaaa cattcacctc cgcactgtta gaagaatttt ccaaggaatc
     6661 aggagcctta gcacaaccaa caagtcttta tatattgtgc agccgatatt ttcttcattc
     6721 agtacaccct tgaaatatgc agtgttagat aaagcagttc gtcctgattt gctgaggcac
     6781 ccatctcttc ttgaacgtac tccttgattt tcaaggccgc ccgatactct ttcggaaaac
     6841 gctgcttcac tgaagccagc aaaaaatcat ccttctccgg caactcttcc ttcttaatca
     6901 ttcgggcaat aaaaaactgt aaatgcgtca caaatcttaa gtaaagcatc gaatctttat
     6961 caaccttttt ttcgggaaac agatatccta gcatagccaa gatatccttt agtgctctta
     7021 ccgagagcat ggtttcttcc atatttgctt gttcactttg ggcatttaca atgtgaaacg
     7081 caatgtttcc agcctcctct tctggcaact gaacattcat ttttttgttg atcaattgga
     7141 tagcatagca gccgatctca aattcttttg ggtaatactt ttgaatttca atcagcaggc
     7201 gattttgaaa aacaatgcct ttttgactgc gttcaatggc atgcttcaag tgatcaacca
     7261 aggtaaaaaa tagactttta ttcagcttaa agtcaaactt ggcgttggca taatcgataa
     7321 ttcgcttgac gcaaataatt agatcatccg gagcagcctc aaacagttta atgtaatcct
     7381 tttttagagc atcagaaagt gaatctaatt taaatacttt ttggattttg tcagccggaa
     7441 ccaaatcgcc tttctttagt tgaaatccaa tccctttccc catcaaaatt acttctttgc
     7501 cttgcttatc taagcaaaga attaaactgt tgtttaacgt tttaattact ctcattagat
     7561 tcaccgttct ctctaagcta agccatttct tgcgattact ttttgatacc aaaagaagct
     7621 ttcttttttt attcgtttta attctttaag attctcatcg gttcggtcaa catatataaa
     7681 accataacgc ttattgaagc cttgatgggt actaacaaca tcaatcgctg accaaggtaa
     7741 ataaccaaaa acatctacac catctgttaa agctgcttga atagccagaa tatgtttggc
     7801 tagataatca attcgatcct ggtcattaat tcgtctatct tcctcaacta tgtcgggtgc
     7861 cccatagcca ttttcagtaa ttaaaatagg taaatcatag cgatcgtaca atttcctcaa
     7921 tgttaatcgt agtccagtag ggtcaactac ccagccatat ggcgttttat ctacataagg
     7981 attttcagct gcccggtaga caccttcttc tcccaacatg atctgttgat cccccgctct
     8041 agcggaaaca tctgatccgt catttttgct ggcagcgatg gtggcagttg aataataatt
     8101 aatcgcaata aaatcgggtt taccggagga taaaattgcc tgatcctctg cctcaatttt
     8161 aggatataat ccacgatcat tcaaatagtt ttcaaacaaa taattgtatt ttccccgaac
     8221 tgctacatct aagaaagacc agcctctcaa tgtttcccaa ttatgggctg caattgcatc
     8281 ttctggactg gaggtagctt gatacatagc cgttaaattg attgctggac cgatttttcc
     8341 tttaagattc aactgatgat acaagtgaat tactctagct tgagctaata aaacatggtg
     8401 attaatttga aagagttctt tttttgaagg cagctctcca cttggtggca aaccaatcgc
     8461 tccaggatga aggatcatcg tgttttgctc attaattgtc agccaatagt tcaccttgtc
     8521 accatactca gtaagcaata cctgtacata gtccacaaaa taatcgatag ttgctcgatt
     8581 taaccaaccg ccctgctcct ctagaaaata aggtaaatcg aaatggtaca tggtgacaat
     8641 cggttcgatt ccctgattca ggcattcatc aattacttga tgataaaaag aaattccctt
     8701 ttgattggtt ttattatttt caatatccat aattcgcgtc caagcgatgg aaaagcgaaa
     8761 agcttttaag cctaattctt taaacagctg tatatcttct ttatagtgat ggtaaaaatc
     8821 cgaggcaact tcaaaattag ctaccccttc tgtatgctga tacttgtcaa taatagacgg
     8881 ttgtttccca tcctcctgat aggcaccctc tgcctggtag gcactaatgg aggcacccca
     8941 taaaaacttc tctggaaatg ctgaaagctg tttgtattgc acaaaaatcc tctccttaac
     9001 taatttgcat caatggttcg tgttccttta agcttccgct tgcttcacta attcctgcgt
     9061 aattttcact gttggtcact accacaatca ctgtatcatc gtagccagcg tttctaatgg
     9121 cagcgaaatc ggcgttgatt aataattgtc ctgctgacac ctgatctcct gcttttacaa
     9181 atacttcaaa gggttcccct tttaaattca cggtgtcgat ccccacatgg atcaataatt
     9241 caataccttc tttagaacgt agaccaatcg catgtttaga ttctaaaata gtaatgactt
     9301 ctccctcaac aggtgaataa atgctgtttt cttctggatg aatggctacg ccctttccca
     9361 atagtccctt agaaaagaca gcatcgttca cttcagaaat aggacacacg gttcctttta
     9421 ccggattttc aacaatgact gcttcggcta ttgccgtttg ctctgattca tcagaagtat
     9481 cgtcaaatcc aattagataa gcaatgataa aggttgcaac aaaggcgatt acacacgtaa
     9541 taattgcgtg aacaatattc attggatttt ctccaataaa ggctggtaag gctgctaagc
     9601 caggggaaac aaaggcatat ctgactaaac cgctcaagcc agcatacaaa ccagcaattg
     9661 caccaccaat catggaagca actaatggtt ttttcagctt caaggttact ccatagagtg
     9721 aaggctcagt aattcccatt aaagcagtga tcccagtgga cacagctagc tgcttcaact
     9781 ctttgttttt agttttcgcg gctacagcaa aggtagcacc tgattgagca atattagaag
     9841 ccagcattcc tggcccattg atcatttcag aacccgtact gctcaattgc atagtagcga
     9901 ttggcgtcat tgcccaagct gtacccgtaa ccaccaaaaa tggctgcaaa ccacccatca
     9961 aaaatggaat taaccaagct gctttgccgt taatccattc agcccccatt gctataagat
    10021 cgttcagcaa cgttccaaac ggtccaatga caaccaacgc taaaggcccc ataattaaaa
    10081 ataccagcat cggcttagta aagaatttaa taaacgaagg cgaaattcga tccgcaaagg
    10141 gctctacata ggacatgatc caaacagtaa agataatcgg taaaactgat cccgcataat
    10201 ctgcagctaa taccggaatt cctagaaaag aaacggcatc gccagtagaa aaaagctgtg
    10261 aaatatttgg atgaagcaaa atgccagcaa cagtaatcgc caaaattggg ttgacatcaa
    10321 actttatggc tgccccatag gctaatagta ctggcataaa ataaaaggct gcatcagcaa
    10381 tagtattcaa aatgatgtaa gtttgactac tgtctgatag tacaccagtc aaagtcaaga
    10441 cggccaaaat ggcttttacc atcccggcac ctgttatagc tggaatcatc ggtgtaaagg
    10501 tggtagaaat gacactaatc aatcttgaaa taatactttc ttgattgtct gaagaggggc
    10561 cctttttaat tgaagttccc acttcctttt gaatttcctt gaaggctgtt tggacttcat
    10621 tgccaatcac cacttggtat tgtccaccgc tatccaccac actaattaca ccaagtgttt
    10681 tttctaaggc tttcgtatcg gctttccttt tatctttgag ttcaaaacgc agtctggtgg
    10741 cacaatgggt taagctgacc acattttctt ttcccccaac ctgttgcaat acatttcttc
    10801 ctacttctct gtaatccatt attctctctc cttaaagcag taaaaaagac caagtcaaac
    10861 cacgccccaa tcaacattga agtgttagtt tgtcttggtc ttgcctgcat aatcagtaac
    10921 atgccaataa cgttattttg ctgtaatcca ataatagcac attatgatag cgcaatcaac
    10981 agggaattta attttttatc ttttcgtttg accgtatagg gtaatataac agttagatca
    11041 catcaactag ggtatatctg cgtatactta ctgctaaaat aagtatatct aatattaact
    11101 ataccagtaa aaccattcct acataaccag cgggaaattc ccaaaatcgt gaaagcatca
    11161 aaaatgcata taacggatca cacaacttca acaattctgc tcttgggaaa aattgatgaa
    11221 tttggtgagt aaaggatgga ataaacagca ttccaacaat ataggcaaca atagtgatac
    11281 ttgttttttt ggtaaaaatc agtaagcaat agccaatttc aatcattccg agatataaag
    11341 gtatcaatag aatgatttgg gctagtattc ttaaaaatta actagcaaat gtttaaactg
    11401 atttatttct aatctacatg tagtaagtat ttcaattcca ccaattatca a
//
LOCUS       DACEMS010000088        11340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEMS010000088 DACEMS010000000
VERSION     DACEMS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.091"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..936
                     /locus_tag="IW735_002024"
     CDS             <1..936
                     /locus_tag="IW735_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1045015.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IW735_002025"
     CDS             939..3122
                     /locus_tag="IW735_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1045016.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IW735_002026"
     CDS             3119..4120
                     /locus_tag="IW735_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1045017.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IW735_002027"
     CDS             4135..5049
                     /locus_tag="IW735_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1045018.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="IW735_002028"
     CDS             5294..5410
                     /locus_tag="IW735_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1045019.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW735_002029"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW735_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1045020.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW735_002030"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW735_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1045021.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="IW735_002031"
     CDS             9380..10642
                     /locus_tag="IW735_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1045022.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11340
                     /locus_tag="IW735_002032"
     CDS             10887..>11340
                     /locus_tag="IW735_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1045023.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKI"
BASE COUNT         3735 a         1897 c         2376 g         3332 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
//
LOCUS       DACEMS010000089        11254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEMS010000089 DACEMS010000000
VERSION     DACEMS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.092"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW735_002033"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW735_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1045024.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IW735_002034"
     CDS             complement(1551..2972)
                     /locus_tag="IW735_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1045025.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW735_002035"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW735_002035"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1045026.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IW735_002036"
     CDS             complement(5383..6639)
                     /locus_tag="IW735_002036"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1045027.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IW735_002037"
     CDS             7219..8520
                     /locus_tag="IW735_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1045028.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8613..10001)
                     /gene="ltrA"
                     /locus_tag="IW735_002038"
     CDS             complement(8613..10001)
                     /gene="ltrA"
                     /locus_tag="IW735_002038"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1045029.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(10488..11087)
                     /locus_tag="IW735_002039"
     CDS             complement(10488..11087)
                     /locus_tag="IW735_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1045030.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         3262 a         2382 c         2013 g         3597 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcatg tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaatc gagtagaggg gaaagccaac tgaccttccg cccctctcac
     8581 accaccgtac gtacggaccc gtatacggcg gttcaataag ttgagtgtac gaactggtac
     8641 ttcaaggaga tgtcctcata tccttggcgg gccagttctt tgtctgttaa tgatcgatga
     8701 agaatatgac ttttggagac tgaccaaata ccttttcttg tatttgacca ttcatacgct
     8761 ttcgctttat cgatccctaa acgaatcaga ttttttcttt tggttcttgg attcttccat
     8821 tgtttccaaa tatattgtct gattcttcgc ttcaaccaac cgtttaactc cctcatgaat
     8881 tctttcatct caccaattcc atagtagttg atccaaccag tcattagttg cttgatttct
     8941 tttaaaataa gttggatact tcttccacgg tttcttttag tgagttgttt gagcttgctt
     9001 ttcacacgct gttttgcttt tgcgtgaggg cgaattttaa tacctttctt tgttggcaga
     9061 atgcagaatc caagaaactt tcgtttcaat ggacttccta ccgcactctt ttctcgattt
     9121 accgtcaact ttagttgttc ttcgagaaat tgaatggatt tcttcatcac acgctgtcct
     9181 gctcgtttgc ttctaacata gatattgcag tcatcggcgt atcgaacaaa tttatgtcct
     9241 agattttcta gcacttgatc aaattcgttc aggtagatgt tacttaacag gggagagaga
     9301 ttgcctcctt ggggcgttcc ttcttcagtt gttgtgatga gtccattttc cattacacca
     9361 ctcattaagt attgacggat cagttttagg acttgtttgt cctttacttt ccgctcaatg
     9421 aaatacatca gcttatcgtg atttacggtg tcaaagtagg ctttcatatc aatgtccacc
     9481 acatatcggt aaccttgttc ataataagat tttgctttct tcactgcgtc atgtgcgctg
     9541 cgatttgggc gaaacccata actattatct gagaattctg gttcaaatag cggttgtaac
     9601 acttgattga ttgcctgttg aagcatacga tcaataaccg tgggaattcc tagttttctt
     9661 acaccgccgt ttggtttcgg tatttctact cgcaaaacag gctgaggttt atagttccct
     9721 tggcgtaaat tggatagtaa ttcctcgcgg tgctctttca agtaaggaag cagttgatcg
     9781 acggtcatcc catctgcacc agccgcccct ttatttctga ctacttgaag atacgcttga
     9841 ttgagattat tccgatctag aattctccca aacagatttt ctacaccatc ttgtttcctc
     9901 tgttcaccgc aagaagtgct gcgcacttca tcataccctt cgtgttccac actagctctt
     9961 tgatgatagc caagttttct tgttttctgc ggtcgtcgca tcttctcaca cctcctaaat
    10021 tattcaagat tcttattgtt cagtccttcg taacggtcag tgtcgttact actatgacct
    10081 cggctgactt ctcatgattc accatcatgt cgccatgatc gttgtgctgg tttgttttca
    10141 ttcaacgccc acaacaccct catgagatct cccccggtaa gagtgaaaac tttcctccca
    10201 tgtaactgct agatttactg tataggtttc gggcagtatt ggactttgtc ttgtgatgaa
    10261 gacttatccg acctaattca gccttgtatc tagtttctgt tcgtcagttc aggagtttgc
    10321 gtccgacttc cttcagccac aacctcacga ttgccgcctt gtctttcgct aacagttcct
    10381 actgccaagc ctgtagtgga ctttcaccac caagttttca cccatgcagg gcgcacacca
    10441 aaaaagccga actgacattt tatcgtcagt ttcggctttt ttatgcttta tttcttcttc
    10501 ggtgcgattc ccttgatcag gacttctgtc cattctgctg tatatggaag cagttttgcg
    10561 tatatctccg gcttagaaat tccttctacc attgcttgaa aatagaggat caagaactca
    10621 tccgaatatt tcaaatccac ttttccttct ttccgccctc gatggaaaag atcaagcatg
    10681 atactgaaac tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt tttttctttt
    10741 tttgtatagt atgtcatcaa atcgatataa aattgtttgt tcactcgatt gagataacgg
    10801 attttgtttt cactcatggc aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac
    10861 gctttcgctt cttgccctaa atcattcata aatgcaaaaa atacttcata aatcagccct
    10921 tccttgctgc caaaatattt aaaaatcgtc gctttactta cctgagcgtt ctcagctacc
    10981 ttatccattg tcactccagc ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt
    11041 aaaatctctt tctttttctc ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc
    11101 cgtaatttta aacttttata atatatatag ttcaaattta ttctgaggtt tattataact
    11161 atctattgag aaagtatcaa gctagcaatt ccttgtttat gaattcattg acagaaattt
    11221 ataaaaagaa tattatgtac taaaataata aaaa
//
LOCUS       DACEMS010000090        11254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEMS010000090 DACEMS010000000
VERSION     DACEMS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.093"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(41..1420)
                     /locus_tag="IW735_002040"
     CDS             complement(41..1420)
                     /locus_tag="IW735_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAQ1045031.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1441..2988)
                     /locus_tag="IW735_002041"
     CDS             complement(1441..2988)
                     /locus_tag="IW735_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1045032.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3237..4016)
                     /locus_tag="IW735_002042"
     CDS             complement(3237..4016)
                     /locus_tag="IW735_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1045033.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4091..4549)
                     /locus_tag="IW735_002043"
     CDS             complement(4091..4549)
                     /locus_tag="IW735_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1045034.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4561..5820)
                     /locus_tag="IW735_002044"
     CDS             complement(4561..5820)
                     /locus_tag="IW735_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAQ1045035.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5936..6250)
                     /locus_tag="IW735_002045"
     CDS             complement(5936..6250)
                     /locus_tag="IW735_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1045036.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6396..7892)
                     /locus_tag="IW735_002046"
     CDS             complement(6396..7892)
                     /locus_tag="IW735_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1045037.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8070..8549)
                     /locus_tag="IW735_002047"
     CDS             complement(8070..8549)
                     /locus_tag="IW735_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAQ1045038.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8925..9170)
                     /locus_tag="IW735_002048"
     CDS             complement(8925..9170)
                     /locus_tag="IW735_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1045039.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9275..10048
                     /locus_tag="IW735_002049"
     CDS             9275..10048
                     /locus_tag="IW735_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045040.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10277..10525)
                     /locus_tag="IW735_002050"
     CDS             complement(10277..10525)
                     /locus_tag="IW735_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1045041.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10624..10938)
                     /locus_tag="IW735_002051"
     CDS             complement(10624..10938)
                     /locus_tag="IW735_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045042.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
BASE COUNT         3501 a         2317 c         1684 g         3752 t
ORIGIN      
        1 atcatttctt ccgttttaaa gaagaaatga ttttcataga tcaaccttta actaaagcta
       61 gattcttttc taaaaagaat ggtgtgtagt agcccacacc gctggacaac ggtaaaactt
      121 catccggcaa ctcgatgccc agttcgttac ggatgtactc ttggcgagaa atgattcgcg
      181 accaaacatt tggatattct ttggctagtt gatcacgaag cgactcatca gctagcgcaa
      241 tcccactctc acagcttgcg ccggcatagc cagcaactga tggaataata tcaatttgta
      301 gtaacattcc ggacttaaga gtaatttctg agtctttttt cattggcgaa gacatccact
      361 cttcatcagc cgtaagatgc cccggattta agtgccattt gtattccgat ttaggcaaga
      421 ctttgcctat tactgaatat agctctttac cttgcattcc tattttgata ttttccaacc
      481 atgcgaccac ggcgtcgtag tatggagctg caactttttc aatgtaatcc ttttgccctt
      541 ctggtaggtc atctgtcgtt gcaactgcat agccacttcg acttgctaat ccgcctttaa
      601 agcccgtagt aattgacatt ttatcgccta gtttaacttg tttatcagtt ggatatatat
      661 aggcgttctc aaaccgttcc ccagttgcag caattggaat gacattattt ggttggccgt
      721 acttaactaa gtggttacca atttccattt ctgtttttcc tacctctact tcatcaatag
      781 cgttcatcac acaatctgaa gccaaggatg agccaaattc ataatatgcc acttcattag
      841 cagtatttaa tgagcgtact ccgtaatcac tgtgaatgaa caggtcggtg cgattgctaa
      901 cggcatcttc ccctactaat tctacaatcg aacttacaat gaatgccggc aaatcttgga
      961 tttggtagtt atcctcttct gaagtaaaca tcttccaacc aacaagccca actttcttac
     1021 ctttactgat tttagcattg gtcaatactt cagttagtgg tttctcgtta tccatcggtt
     1081 gatccggtaa tgagaagagt ggcgtgtgga ttaattctgc ctcaattctt gaatagtcca
     1141 ccattttagt attttcattt cctagaacta agaaagcttc acccgtctga tgtaatacca
     1201 acaagctttc ttcaaagcga gtaacaaaac ctgttaaata agagaagttc ccgccatgtt
     1261 ccaaatcaca atagattacg agggtatcta tctctgactt ttccatcgct gcaagtacct
     1321 tttctttacg gaacttcatt gtttcatcag tcaattgata tattttagct tcattatctt
     1381 ttttaggtgg gttaactttt tttagagtta ccttagtcat attgtcacta ctttctgttt
     1441 ttatttacgc ttcatttcat ttttcagaga tcgagttgcg ttttccactt gtcctccata
     1501 taccacttgt aaattatttt cgttaatttt aacgattccc attgagccgg tctttttcaa
     1561 gatatgttca tcaattgttg ctgtatcttt aagaactagt ctcagccgag agatacagtt
     1621 atcgatttct tgaatattac cagcaccacc aagtccttgc aagattagtt tgccaagttc
     1681 gttttctgtc atttcttcgt cctgatcttc caacacatca tcgttggatc gaccaggagt
     1741 tttcacatca aatcgctcaa tgacatactt aaatacaaag taatatgcaa ctgcataaac
     1801 aaggcccaat gctacaacta agtaccaacg agtataagta ccttgtaaaa ttccgaagat
     1861 agccaaatca ataaatccac caccggcatt cccaactgcg actccgaata gattcattaa
     1921 cataaaggat aaaccagcca tgaatgcatg gaagacccat agaatcggtg agataaagat
     1981 aaaggcgaat tcaattggct cagtaatccc ggttacaaaa caagtagccg ctgctgcaat
     2041 caacatccct ttaatctttg gacgttgcgc caaatatgaa gttttgtaca ttgcatagca
     2101 tgctgccggc aatccgaaga ccataaatgg atgagtacct tgtgtcaaga agcgagtagc
     2161 acttctcaaa atatccatgt cttttactgg caatgctaat tcagtattga agatggttaa
     2221 tgctccagaa actgtttcac cattgatagt tgccgtaccg ccgattggtg taaatcgaat
     2281 caactggttt agtacatggt gaagacctgt tggaatcagc aggcgctctg agaagccata
     2341 taaaaaagtt ccaattacgc ctgacttacc aatcaaaccg cctaaagcat tgatagctgt
     2401 attgacaaaa ggccagatga aaaatgaagc gattgagatg attggaatag ttaggatgat
     2461 cataattgga acaaaacgat tgccactaaa gaaactgaaa gctgctggta attgttttgt
     2521 gtgatagcga ttgtggatca tggctgtcca taatcctgca atgataccgc cgaaaacacc
     2581 catacgatat gtgaatgtgc caagtaaagt ttcatacata ttggaagttt ctactgcttt
     2641 gatttgactc attccgtcag acatcaatgt ttcaacagaa gtgctttccg ctgtgatgcc
     2701 tctaagctga agcagataat tgataactac atggaacaga ataaatgcaa taaccactga
     2761 aaatgctgca atttccttat cttctctaga catcccgatt gctactgaaa tagcaaatag
     2821 aataggaagt agtccaaaca tcgtaccaac aatagcctgt actccaccaa caaatccttg
     2881 gatcacggtt gcattagcga agctttctcc caccaaagta ggattttgta aaattgaaac
     2941 gattcctagt agtagtcccg atgcgacaat aatagacaac ggacccatta atgatttccc
     3001 taacttctgg atataagttc tcaatataaa caccttcttc tttttagttg atataaacgt
     3061 ttacatccct tgtacaacga aagtatagca ccctgaaaaa gcgctaacaa tacgataggt
     3121 ctgataaaaa tcaacattca gcacttggtt gttcattcaa aacgtgaatc ttcaatcaca
     3181 gaaaaatacg cgctatcaag cacgtatttt aggtgtaaat atatgcatca actttattaa
     3241 tcaacgctac tttgtttgta atagttaata tacccttcat acagtaagct aagagcaaga
     3301 ttcaaggtgc agaatgatga gttgttcaac tcattcacat cattaatgtt ggtcacttga
     3361 tagtataggc tatagtctga cagactggct aagttatttt gaccaaatcg tgtaactgag
     3421 accaactgtg tccctttcat atggattctt cttaaagtat catcaatccg atcaacacgt
     3481 ccggacaatg atatgacaaa tagtaaatca ttcgaggtca aagcttttga aatcaggtct
     3541 aattcattct tttccggtac aacgataatg tagattccca agttccacat gcagcgtgca
     3601 aactcattga gcatcattct ttgtccatat cctgttgcgt aggcaaatat cttatctgcc
     3661 tttctaatcg tttcatatat atcctcaaca cttttatcat cttcaaacaa tttggttgtc
     3721 gcttgaacat ctccgagtaa tacttccggt aaaaacaaac tacttttatc gactctggtt
     3781 ttttgcatgc gtgcaaattc ctctttaagg taatatcgaa attcgccata tcctttgaat
     3841 ccgagtttct tagttagtct aataatcgtt gagtcagaca catttgcttg ttttgctaat
     3901 tccgatacag tttgactgat gctgatttcc atattttgag tgataaaact taatacataa
     3961 tcatcattct cattcaattt actgtgatgt tcatttacta attgctccaa tgtcataatc
     4021 agacctccaa aaataataaa aaagattgct ctctcatttt aatacgaaag cacaatcttt
     4081 ttaagtacta ttaaattttg aaaagaagtt ctccttcaga aacctttttt tcagagactt
     4141 ctagtttaat acccttatct tcgggaaata gaaccataac aacatctgac agtttttccg
     4201 ccttgatttg gtcacaatca aaaatcacca atgactcgcc ggatgatact cgttgacctt
     4261 cttcaacgag cacttgaaac ccgactccct ttaactgaac agtatcaatt ccaatatgaa
     4321 tcaataaatc tctaccttga ttactagtca atgtgagtgc atgctttgtt ggaaatatcg
     4381 atttaatgac accgtcaatc ggtgctacca ccttagcgtt tgttggttca acagcgaatc
     4441 cttcgcccat catcccttgc gaaaatacag gatcgcttac ttggctaagg ggaatcagat
     4501 ttcctgatac tggagcaact aatttttctt ttcgactaaa aaatcccatt gacttactcc
     4561 ttaatctttt gaaatgtcca tcacaaggtt taaaagaaca tccgcccctt ttttgacatc
     4621 ctcaaaatca gtccactcat caggatgatg tgtaatgcca cctttggatg gaatataaat
     4681 catatttgtt gggacaaagt cggtcatcat catcgcatca tgtccagtac cattgttaat
     4741 gacttgatag ctatagccca actgatcgca aatatgtttc atcttctcaa cattaccttg
     4801 atccataaca cttggatttg taggattact gtagttaatt cgagccattt cctccagctc
     4861 agttctaact ccagtttccc cttgaatcac tgataactca ttcaacaact tttctttaat
     4921 ctcttgagga ttagttgtct ttgtatcaaa ggttctgatt tcgattaaag cttcgacaaa
     4981 gtcagggaca aattgatttg agttcggttt gattgtaagt ttaccgacgg ttccggcaat
     5041 cccttcacca agattcttca tccactgttc aacaaaaaga acaaatttag aagcagcaac
     5101 taaagcatct ttccgtttat tcattggagc agtcgagtcc tcagtttctc cataaaaacg
     5161 tgctttaaaa cggccgatgc caggaataaa ttcaatcaat ccgatatctt tgccttcatc
     5221 ttccaggact ggtccttgtt cgatatgaag ttcaataaaa tttttgattg tttttggatc
     5281 tcgcttagcg gcctctaaat ctggttccac tccataagca cgaagagctt ccaattttgt
     5341 ttgtccatgg cgatttttta ctgtttccaa ctcactcatg gttaatgttc ccatcatagc
     5401 tcgtgaattt gagacacccg tacttggccc aaatgtctcg ccctcctctg cattcatcag
     5461 aattaggtca attgggtaat cattttcaaa attattttct tccagaatcc gcattgtttc
     5521 cagtgcagta acaacgccag ccactccgtc aaatctgcct ccattaacaa ctgaatcata
     5581 atgagagcca atcatcactg atggtgcatc agccttttta ccggctctac gaccaaataa
     5641 tgtagagaag ccgtcttccc aaatctctaa acccagttgt tccatttgat tgatcaaata
     5701 atttcttgct tcacgatctt ctttagagta agcagagcgg gtcactcctt cacctggagt
     5761 tgcattgtac tcggctaaat tttcgatatc cttttttata cgctcaaaat ttgtcttcat
     5821 aattaaatcc tccatttcct acaagtgaat gttagcaccc atagaaagcg gctacaatat
     5881 aatcaatgaa gattgaatcg atattcgaaa actgaatatt gcttcggtta atgacttaga
     5941 aatcattatc gtagattcca ccataagaac tttttctcgt ctcataacac aactcgagag
     6001 tggattggtt taaaaaactc tcgataaatg cgtccacttt attgacacag atccatggat
     6061 cgatcgcacc ttgaaattta aacccagaaa tagtcccatc atcttctata ccgattacca
     6121 gcttacctcc atcggcattt gcaaacgcta ctatgtgttt tataatatct ctaggcttaa
     6181 tccgtgcact ttttctatca aaatactgat cttctttttc acttataaac ttttgaatct
     6241 cataatccat ttaaaatttc ccttcttaaa tttttttatt atagagccgt atattcaatt
     6301 tccacaaaaa aataatctaa aacaaaacag acaccttcat tttgatatgt tctcccacat
     6361 atgaatcggt cgctatacag cattacatat agtaattatt catactgcga aataaatagc
     6421 tccaaatctt tagctggcgt agttaagctt aaattcgaaa tgaaagtttc attgactatt
     6481 ccactttcat caaatccctt cttcaaccaa tcagagttcc acaacgtaaa tcctttagga
     6541 ttagaaactt tcttacataa ttttgcacta taggatttct ctcttgaatg aattaaagaa
     6601 agtctttgta accaaatttc aactatatcc gtattaggag tattaacaaa ttttcttaag
     6661 attttatcca agtataagtt gatttcttct acagcaataa actcaaaaag cttacctaaa
     6721 atcacaatac aagcctccaa agttctagga ttcttttgca ttatattaac aagaatacta
     6781 atcaattggt agatatcatc tggtcttttg tttaaagtag aaactctatg tgtatagact
     6841 tcacttagcg ctttacttaa actcccgcaa tttggatatt tgtctcctaa aagctttacc
     6901 tgtagcaaat gcttttgaag gctaagctta aacttccact tattattact tttctctgta
     6961 aaacttgcat gtttaagatc ccagtaaatt ttatcaggct taattgcatc taaaattata
     7021 ttatccgata agaatgtttt atttgaattt agcttcaaat ttagatccga taatacttca
     7081 gataacaatc tgataatcac ttcagcttga tcttttcgaa ccgaaaaaat tctgtaatca
     7141 tctctgtaac gaagtatttt gaactcttta atctctgttt tttctatctt gttcagtaac
     7201 attttatcag catacccaag tactatttca gctacaaaat ccatcaatac atttccttga
     7261 ggtataccgt tcgtttgtcc attttgtaaa tatcgtattt tagaatctat taagtttcca
     7321 acgccttgag tatcttttct atgtgtctta gcccattctt ttgaatgtac tgcccacggg
     7381 atagtatgtg tgtacattga gctataacaa tcggtaatat ctgaatgaat gcagtagtca
     7441 tactccaaag cataactaat ctgtgcttgt tcaaggttct cccaccaatt taaaatagtt
     7501 tccgcagtat cgctctttga agaaatagat tccacaggaa gtgatataca ttcaattcgc
     7561 ttgtcggctt ggaattcttc aaacttattt ttgatgtgat tccaattatt tttctgtgta
     7621 atcccgttta ctaagtctac atatactaat ggatgtaata tttgaagggg tctccaagca
     7681 taattaccat ctttattagc taatattcga tgattaacat caactgcctc agagagcgat
     7741 ctcttatgtc taactagttc agtaaatggt ttgtttttta ataaatccac tgctttctca
     7801 atgcctactg ataaatcaat atagttgggc aacgatgtac taaaaaaact tgatgttttt
     7861 aaaaagaatt gctttgcttc ttgatgattc atctctaaaa tactttttct agtcctagtc
     7921 aaattcaaaa tctcctagat caattaagtt gtaatgtaag aggataccct aaggttacac
     7981 accctattta ctattatacc agtattcatt cgtgatacat aagggtgtaa cattaagaac
     8041 tctatctaaa agactacaag tagtcacctt taattgtgag taattttctt tatccaatcc
     8101 ttgtgcaacc ttgtcgtcac cccttgtata tccccgtacc ccggatgttt cggaatatca
     8161 aaaatgtgct caaattcctg attttcatca gtctcccact catgattcaa cggttcatgc
     8221 cactctataa accttgacca taaaatgttc tttctataaa actcaatagg aatttcaaaa
     8281 cagtaaaaat tgtatttgct aaaatctgta aaattatctt tacgagcata ctttaaatgg
     8341 caggtatctc gccacaacca aattaaatct cgatccaatg gtaactgaat tcgtttcgta
     8401 tactcttctt caagccaatg aagtgcacca actgcattcg gattatcgaa aaagacagat
     8461 aattcagtat agcgtttctt tggataaata aatccttctg aatcaacaga ttgcaaaaaa
     8521 tctactggtt gaaccgtgta cactttcatt tattcgccac ctttttttga ctacttatag
     8581 tcatttacca atttgaaatc tgcatacttt ttcattcaat tgagcccagc attaattgta
     8641 cacgagtttt cttcactaac ggatagtatc aggatatact aatactatcc tgaatagcga
     8701 aatgaggtag ccaaaatgag aaaagtcgat gaatatatca aaaagaaatc ccaaaaaagt
     8761 aaagtatggc aaagaacatt tgagttagag tataagaaac tctcagatag cctcaaaaag
     8821 gacacttata atccaaatga acaaagtaaa gcagataacc aataaagatt accctcccat
     8881 atttaacatt gcttcacgaa tcagtaatca atgactacaa gtagtcattg aagcaaatct
     8941 tcataagata cctgtagaat atccctaata gctctcaatt gatccagctt aatatgttgc
     9001 cggccacctt caatcttaac caatgtttct cttgtcatag cgattttact gagctggagt
     9061 tctcgcacca gttctgtctg tcccattccc ttttctaatc ggattcgtct gatattgcta
     9121 ccgattacat tttctttatc tattaacttt tcttccttca aaaatagcac gccttttgga
     9181 ctaaaattag tcctttttct tattaatctt aaagatttgg tatgctacaa tgggactagt
     9241 attagtccat attcaatttt aaggaggaat ttttttgaag aaatttgcaa tcattctaac
     9301 ctgtgcttta ctcacgggag ctataagtgg gtgcgccact tctgacacaa attctacaag
     9361 tactgaaaca agtacaacaa cctcagaaaa gaattatcct atacattttt ttccagttcc
     9421 agatggattt gaagcttatt atcatgataa aaaactagat gttccattgt cagaaagtaa
     9481 ctctattgaa aaagttttgc caattgaaat tgaaaaaaat caagagaata taaaactcaa
     9541 ggccgagctt cccatactga acagcacaga aattgattgg cacgagaata ttaaagtaac
     9601 tattgaaaaa ggagcgttag cacaacttta tactgaaaaa gttgaacaat tttttgagtc
     9661 tggtgccaat aaagactttt caaaaataag cgattattcg gataactttt ttaatgagac
     9721 ccaagatgaa atcacgaact caacttttat ggctgaagat atgtacaata tgacgttaaa
     9781 aaaaataaaa gcttacgacc atccattaaa tctgacacta aataatggat tactctcttt
     9841 tgtattagca ggttacgttg actatgaata ttcaccagat tatttggaga cacctcttca
     9901 acacgagaac aatgatatta attttagatt tgtctacgag tcaaaaagta aaacatggtt
     9961 agttgatcaa ttaagcacta taaacacaag cttccatgaa ttacctaaac ctgaaaatga
    10021 agctgatatg gtcattaaaa tcttctaaaa aagaagcgtt gaaggagaaa tattcctttc
    10081 aacgctcttt tttatatgct tagaaacata aataaattgt atactttctt tttagcccct
    10141 attacttttc acgaaaaata tcttttttcg acactagcgt ttctctactt ataatattgt
    10201 acacagtttt tgtattattg aaaaattact cattatttag cattaatcca catcgtttta
    10261 tcaatcgacc ttcatactaa actactttaa agtcatcttc cttctcattt ttcattaaat
    10321 cagatacata agcccatgta cccgttcgtt gtaaataaag ttcttcttgt agcagctccc
    10381 actttctctt agaaatcata atgacgccat cgttcaatcc atttttagaa tccctagtaa
    10441 tttctatttc agaatttgaa tgaactactt cttgcaacac tttacctata ttttttttga
    10501 agtttgttgg agtaatagta tccaaaatga acacctctct tctctatacc tctattatga
    10561 agcgcccatc aaataaagca agcgttgagg aataattctt tcaacgcttg ctttattaag
    10621 ctttcatttt gtagtttgac taataccaat aatctgatct ctaaacaatc gaaaaacttc
    10681 ttgtggttga taataatctg tatctatttc aaatgtttct gagaacaatt cattgaattt
    10741 ttctactgaa atgatgattt taccattctc ctctcgactg ttttctatta tttctgcttc
    10801 taattcaaat aaatctagtg acgaactaaa atcataaatc ctgtcatatt gaacaaaaaa
    10861 atcaagaaaa agttgctcaa actcttcctt atttatcact agtgtttcaa gcggaacaac
    10921 tttattttca tcagccattc ttccaatttc ctcactttca ttttcaaaaa atcgtaaaat
    10981 tgatatagat tctttgtctc taatcactcg tgatattatc ttgacagtcc tacatctaat
    11041 tcaattaaag tataagccac tttcaaataa aagattacaa agtaaatggc aagccaacaa
    11101 aataaccatc tgcttcacgt attacgattt gataattctg gatttcgctc ggaagttcat
    11161 aataataatt aacagtttct gctttgccac tttcaatata ttctggatta ttcaatatca
    11221 tagcatactc ataaagttct tcaccatttt tatt
//
LOCUS       DACEMS010000091        11103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEMS010000091 DACEMS010000000
VERSION     DACEMS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.094"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            52..789
                     /locus_tag="IW735_002052"
     CDS             52..789
                     /locus_tag="IW735_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1045043.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            817..1356
                     /locus_tag="IW735_002053"
     CDS             817..1356
                     /locus_tag="IW735_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1045044.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(1854..2315)
                     /locus_tag="IW735_002054"
     CDS             complement(1854..2315)
                     /locus_tag="IW735_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1045045.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
     gene            2448..2822
                     /locus_tag="IW735_002055"
     CDS             2448..2822
                     /locus_tag="IW735_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ1045046.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            2876..5002
                     /locus_tag="IW735_002056"
     CDS             2876..5002
                     /locus_tag="IW735_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045047.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIF
                     VSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFM
                     IFLINLITSIIIYKKNSFKYFKENIL"
     gene            4999..5619
                     /locus_tag="IW735_002057"
     CDS             4999..5619
                     /locus_tag="IW735_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1045048.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            6312..6692
                     /locus_tag="IW735_002058"
     CDS             6312..6692
                     /locus_tag="IW735_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1045049.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            6674..7588
                     /locus_tag="IW735_002059"
     CDS             6674..7588
                     /locus_tag="IW735_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1045050.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            complement(7844..8992)
                     /gene="tnpB"
                     /locus_tag="IW735_002060"
     CDS             complement(7844..8992)
                     /gene="tnpB"
                     /locus_tag="IW735_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1045051.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(9009..9413)
                     /locus_tag="IW735_002061"
     CDS             complement(9009..9413)
                     /locus_tag="IW735_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1045052.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            9719..10408
                     /locus_tag="IW735_002062"
     CDS             9719..10408
                     /locus_tag="IW735_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045053.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(10529..10615)
                     /locus_tag="IW735_002063"
     CDS             complement(10529..10615)
                     /locus_tag="IW735_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1045054.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
BASE COUNT         4127 a         1744 c         1723 g         3509 t
ORIGIN      
        1 ttttacatcc tcttatgtct atgataacat aatttattga ggtgattttt attgagtaac
       61 gatatagttc gatataataa tggattaaat tctgtccctt taagaaaatt tacccctata
      121 gatatggatt tattttgggg aatatgttct aaaatgaaaa gaaaaggtac aaaagaagaa
      181 atttttacat ttaaacaaat aaaagaatta attgattaca atataaatgc acgtggcgac
      241 cgttttgcta atgatttaat aaaaatgtca aaaaaaatca ttactttgtc ttttatttat
      301 gaagacgaaa aaacatatga acaattaaat ctgtttcaaa aatttaaaat cgataaagtg
      361 aatgaaacat tattaataaa ggtttctgaa gaatttgaat atatacttaa ttcaatcgga
      421 actaatttta ctcgtttcga attagaaaat atgacaaact taaagagtag ctacgttaaa
      481 gagttatatc gccaacttat ggctcataaa gacagaagat ctaaaaaagg tcactggttt
      541 gtaaaaatag aagattttag agctctatta gcaataccag aaagttatcg catgagagat
      601 attgataaac aaatttttgc ccaagcgaaa aaagaatttt taaacgttaa ttcaactcac
      661 cctgctattt ttaaaacttt tgaaatcaaa aaaattaaag cctacaaagg aaataagata
      721 ggatctctgc aaatttattt tgaagaaaat ataccttcaa ttaccttaga taattggtta
      781 gagaaataat acctaacaat ttaggaggca attatcatgc aacccaaaga aacctttaca
      841 ataaaggaat tatctgaatt atttgaaata agtaggcaag caatctctaa acatgttgat
      901 aaattagacc cgtccttatt agctaaaaat gagcgtggtt ataaggttgt attacgttct
      961 ggagtattgc aacttgctag caacttaggt aaccaaaggt tactatatag gttgcatgag
     1021 tatgaacaag aaaacgataa aaacgtttat atatcaagca aaaacgtgat tgtaaacgag
     1081 ttgctagata agttgcgtga agaaaatgaa tttttacgag aacaactttc cctcaaagag
     1141 caaaataatt ctaatatgca aaaattactc gaccagcaac aacagctcac cctacaggca
     1201 aatcaacaga tcgaaaaact gcaagaacag ttacaattag cttatacgga agaaactcca
     1261 gaagatgaat caactactat tctttctgaa gaaacagaat cgatagaaaa acacacaaaa
     1321 aagaagaagc aaaaatggtg gcaactttgg aaataactgg agggaacatt catgacaaga
     1381 aaaaatcttc tattcggttc tattttacta ggaattctag gcttcagtcc gatgttattg
     1441 tcagtaatca ataaagggaa taaacatggt gaattgaaat tgaatccaaa ttattacaac
     1501 agtcctaacg aagaattgtt tgtaaaagag acggataaat aataaaaaag actgctcttc
     1561 cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa aaagcacgcg cttataaagc
     1621 gctaccaaaa ttttatcata ttttataaaa aataacaatc cagtaaaaaa tgactggtaa
     1681 aaattcacaa agaaacggaa agaagcaact acagaaaatc aagccttaaa aagagctttt
     1741 tacctagacc aattaaaaat cacaaagtga gcgtctctat ccattggtag aggggctact
     1801 tggcatagtg tacattatgt caactatgca attaaataac taactacttc atattagctt
     1861 aataaaataa tcaaaatcac taggggaaaa aatattgtat aaaaaaattt ttcatttttt
     1921 aaataagaat agtaaatata aaaataattt aagatatcta ttactattaa tccagataga
     1981 gcaaaccaag taaatattcc gtgtgtaaag ctaaaaaaat tcatttgaat caattcaaat
     2041 aaacctaata tagtaacaaa tatattaaaa ataatagctg tcaataccag taaattattt
     2101 tgtttatttt ttaaattatc ttcatttaaa atagaattta tgttagtact ataaaattca
     2161 caaatttttt ttagtgtctc tatatctggt gttgttttgt tattttccca ctttgaaata
     2221 gtttgacgtg taacattaaa ttttcttgcc acatattctt gagtataccc ttttttcact
     2281 cttaattctc ttagcttaga tcctaaatcc tccatttttt ctcctatgta aacgattatt
     2341 tacataaaat tttatcataa aaatactaac gcgccaaaaa agttctttat tttataaaac
     2401 acactaaaat aaaataattc tagtaaaaaa gataggagaa ataagttatg aaaaaatttt
     2461 tatgtctttc tgctttaatg ggtgtcttat taactagtgg aggaatagtt agtgctacag
     2521 aagctttaaa tctagatgta cccgaagacc ataccgccat ttacggagga ggaatggaag
     2581 ctacagaaga aggattttct tctagtaaat tgagatatgc tgctggcggc ggagatttca
     2641 actgtggggt taatggattt aaagtttatg caaattacta ccatgctaga gctaaacata
     2701 gtgcaacagc taaaaatgga cgaggcggtc aagtacgatc agttcagaaa gctggagtaa
     2761 gagcttatgc aacatgcaat gccacactta ctggaaatac tggttggtgg aatgtttatt
     2821 aaaaaagaga gtttaaactc tcttttttat ttacatttga aaggaaattc aaaatatgtt
     2881 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat tcttttctac
     2941 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
     3001 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
     3061 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
     3121 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
     3181 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
     3241 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
     3301 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
     3361 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
     3421 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
     3481 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
     3541 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
     3601 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
     3661 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
     3721 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
     3781 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
     3841 aaatatccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     3901 atataaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     3961 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     4021 cgcgccatac aattacgcaa ctatccaaga aaataataaa gaatttttta ttggacaaaa
     4081 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     4141 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     4201 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     4261 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgaag actctcctcc
     4321 tcaagctcct tataaaactc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     4381 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     4441 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     4501 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     4561 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     4621 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     4681 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     4741 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     4801 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     4861 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     4921 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     4981 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     5041 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     5101 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     5161 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     5221 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     5281 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     5341 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     5401 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     5461 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     5521 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     5581 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     5641 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     5701 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     5761 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     5821 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     5881 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     5941 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     6001 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     6061 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     6121 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcaccaa
     6181 atacggaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     6241 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     6301 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     6361 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     6421 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     6481 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     6541 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     6601 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     6661 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     6721 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     6781 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     6841 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     6901 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     6961 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     7021 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     7081 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     7141 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     7201 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     7261 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     7321 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     7381 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     7441 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     7501 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     7561 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     7621 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     7681 aaggtgaact actcaccact tagctaaacc tgacggttct taacgcttga agtgggagct
     7741 tcttgggaat agagcatact tgatagcgta tttctttact aacagcggtg tctcttattt
     7801 accaagctat ccccgtagtt cctacggttc ttgatgattg gatctaagcc aatcccatag
     7861 aatgtagtct tagaccttcg gtcagaatat tgatactagc attgatatca cgatcatgat
     7921 gagtatgaca aacaggacaa gtccattctc gaatttcaag agatttcttg ccatctttat
     7981 gtccgcactc tgaacaaatc tgactagacg gaaaccattt atctactttg atgatttttc
     8041 gtccatacca gtcagccttg tattgtaatt tagtcacaaa ctttgaccaa gaaacatcag
     8101 aaatactttt agctaattta tgatttcgca acataccttt tgtgtttaag tcttcaatac
     8161 agataatatc gtgatttttg atcatttctg tactcaactt attcagaaaa tcagtacgtt
     8221 gattcattac tttttcatgc aatctagcta ctttgcgttt ttgtttttga taatttttag
     8281 cttcaaatag attggtacct ttatttttag ccaataaagc acgcctagac aatttgcgtt
     8341 gttcacgctt tagtttcttt tccattttgg acgtgaattt attattatca attttttgtc
     8401 catcagaaag aatcgcaaag tccataatac ctaagtcaat accaattgtg gaattagttt
     8461 taggtaattc gataatttct tctttacaca acaaagaaat atagtattta ccgctagaat
     8521 gacgtgatat tgtagcggat ttgataatcc cttttggttg tctatgaagc ttaattctta
     8581 ctaatgactt caatttagga actttgatga atttactatc aatcaaagca actgtaccat
     8641 tttgattatt cgttgtataa ctctgaacag gatttttctt acttttgaaa cgtggaaatc
     8701 caacggattt atcccgaaaa aaattcttgt atgctttatc taaattaagt tgggcattgg
     8761 ctaaagcaag gctatcaatt tctttcaaaa acggaaattc tttcttgtat ttagctggtg
     8821 tcggaaaagt cattttttta gaagaatcat ttttaacttc ttcatacgcc ttttttcggt
     8881 catcaagcat tagattgtag accttacgga cacaaccaaa agatttggca aagaagattt
     8941 cttgttcctc tgttggatag attctgaatt tgtatgcttt tagttgttcc ataaagttca
     9001 cctactttct atttatgatt gtttccttga ttttggatat acttcttaat cacatcaatt
     9061 ggtgcgccac cagttgtcag aagacaaaaa cttttagacc agaacatttc tttccaaaga
     9121 aattgtttga ctcttggaaa gtcacgtttt atcaatcttg agctggcact tttataggca
     9181 ttgatgaatt ttgtcaattc tgttttaggt tgagctttga acataatatg aacgtggtct
     9241 ttatcatgat tccactccac taaagtaatg tgatacgatt ctgaaattct ttcaaaagta
     9301 gttttggcat aatcagatat ttcatcatca atcacttgtc tacgatattt cgttactaaa
     9361 acaaggtggt aataaagaag aaatactgaa tgattatttg tatctaattc catgttatat
     9421 caactcattt cttgtataga ctgattgtaa catggaacaa aataaaaagg caatacgcct
     9481 tagttgtcgg tcttcgagtt accaacggta accctcatac cgaacggcat tcatcaccca
     9541 cctatagagg atgggcgact tctgcctaat tctgttaaaa aactccatca agagctatta
     9601 gaccagcgaa ataaggaaca gaaaaaggtg cttattcaac gatcattgga ttttcttgcc
     9661 cccttatgtt gcttagtcct gacactgttc gcattttggt tagtatgggg aggctaaaat
     9721 ggcattttat ttaccctatc tactcatttt tgttagtatt tcaggaagta tttggctcat
     9781 ctataaaatt tttcaaacaa gatattcatt aaaaggctct aaaattcgat ttaagcgatt
     9841 tttctttctt ggctgtatat tttccttgat aatcgtttca agcggcttat tcggcgtttt
     9901 agagggaaat aagcgcgttt ctagaagtat tttattagga aatgtcactc aaaagtatga
     9961 atcagcgcgt gataaaaaga aaaaagagca agcattagca caaaaaatgg aagagtttac
    10021 cacttgttat gaggaaatga acgatatttt tgtgaatcaa gaaaaacgtt tgacggataa
    10081 aaacatggaa aaactcactc gtctttatca aaacttgcca gaaaaacagc aaaaagaggt
    10141 tcaagacaac tatgagcgaa ccaaaaaaga cgtgcaatat gtaaaagata caaagataga
    10201 agaagcctgt agtgatctat ttggagacac aaacccttgg tttgccagtg aagaagaaaa
    10261 aaaagaaaaa caacaatctg tcacatatga acgctatgaa atcttacttc aacaagcaac
    10321 taacatgcaa aatccgacaa aaaaagaaac cgccttaaac tatctaaaaa gtgtcaagga
    10381 atggttagat cagcaacaac aaaattgaat ttgatttaaa atcatgttaa actgaatgtg
    10441 caaatgatca aacattagat acacgaaagc cccagagagg tcggattctc tggggctttt
    10501 tttcaggttg atgtcgcctt tattgatttt acttcttgtt tcggttattc aaccaataac
    10561 tgaataaaga gacacaaact cctacaagaa gggggcaaat gaacagttca aacattagat
    10621 acacctcctt ccagtcgcaa gactgtcgga taggcgacaa gttattatat cttaaaaaat
    10681 taaatttgtg tggtgttttt aagtatcact tgttttaggg ttgttagggc ttgccctgac
    10741 cgcctgtaag gcgcttctcg actagattaa gggaaattaa tcaagaattg tcttagaacg
    10801 gcttagacaa gctttcagag tctcacacct cctctttgaa caacggtttt agcaaaggag
    10861 ccggaggcga ctgcgcagta taatatgaga gcatagcgat cacacctttt tagcgaccgt
    10921 agggagcaag ttttccagtt tagaaaaatt cgcgaacctt tttggaggaa aaagtaggag
    10981 ttttttaggc gggaaattca atgtgctctt aagatatact gttagatata ctgttagata
    11041 tactgttaat aataactgtt agggattagc aaccttatgg gagagtaagt attgacatag
    11101 gta
//
LOCUS       DACEMS010000092        11089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEMS010000092 DACEMS010000000
VERSION     DACEMS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.095"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(135..1715)
                     /locus_tag="IW735_002064"
     CDS             complement(135..1715)
                     /locus_tag="IW735_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1045055.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2116..3492)
                     /locus_tag="IW735_002065"
     CDS             complement(2116..3492)
                     /locus_tag="IW735_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1045056.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3620..4738)
                     /locus_tag="IW735_002066"
     CDS             complement(3620..4738)
                     /locus_tag="IW735_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1045057.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4880..5314
                     /locus_tag="IW735_002067"
     CDS             4880..5314
                     /locus_tag="IW735_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1045058.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5392..5925)
                     /locus_tag="IW735_002068"
     CDS             complement(5392..5925)
                     /locus_tag="IW735_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1045059.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6019..7197)
                     /gene="mutY"
                     /locus_tag="IW735_002069"
     CDS             complement(6019..7197)
                     /gene="mutY"
                     /locus_tag="IW735_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1045060.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7190..7987)
                     /gene="recX"
                     /locus_tag="IW735_002070"
     CDS             complement(7190..7987)
                     /gene="recX"
                     /locus_tag="IW735_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1045061.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8094..9476
                     /gene="rlmD"
                     /locus_tag="IW735_002071"
     CDS             8094..9476
                     /gene="rlmD"
                     /locus_tag="IW735_002071"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1045062.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9650..10228)
                     /locus_tag="IW735_002072"
     CDS             complement(9650..10228)
                     /locus_tag="IW735_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1045063.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10550..11020
                     /locus_tag="IW735_002073"
     CDS             10550..11020
                     /locus_tag="IW735_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1045064.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         3302 a         2226 c         1912 g         3649 t
ORIGIN      
        1 acatttctat gattttaata tagtaaaaag agccgtcacg gaaaatccgt cacggctcta
       61 aacaaaatat gagaggctgt ttttgtttgg ctattcttat tacttgatgc agggcagctt
      121 gtgtcaagac ttctttacat caaacttttt aattttacat tgggatattt atccgcaaac
      181 cagcgttctg caaattggtt ctcgaataag aacaacggtt ggtcgaaacg gtcacgagcc
      241 aagatattgc gacttgaact catcttctca tccaaatctt ctggatcgat ccagcgagcg
      301 attttgtgtc ccattggaga catgattact tcggcgttat actcatttaa catacgatat
      361 tggaatactt cgaattgtag ctgaccaaca gctccaatga tatattcttc cgttagatat
      421 gttttataaa gctgaatcgc tccttcttga actaattgat agattccttt gtggaaggat
      481 ttttgtttca tgacattttt tgcagtcact ttcataaaca gctctggagt aaatgaaggg
      541 agttcttcat aagcaacttt tagttttcct tcgaacaatg tatccccaat ctgataattg
      601 cctgtatcat atacaccgat gatatcgcct gctactgctt tttcgacgtt ttctctcgaa
      661 tctgccataa attgtgtgac attgcttaat ttcagttttt tatttgtacg ttccaaccag
      721 acatccattc ctcgttcgaa tgttccagaa cagattcgga caaaagcgat tcggtcacga
      781 tgggctggat tcatatttgc ttggatcttg aatacgaaac ctgaaaattc ttcctcataa
      841 ggacttacca tctgatcatc ttgtgttttg tgtccataag gagctggtgc caattcaacg
      901 aaagtctcta aaaaagtctg tacaccgaag ttagtcaatg cagaaccaaa aaatactggt
      961 gtctgatcgc cacgagcaat tttttcttga ttaaatgcat ctccagcctc tttgattagt
     1021 tctacttctt ctaagacttg ttcatataag gaattttcag ttagaggatg attttcggga
     1081 agcagtccat cttcatccaa ttctactaat cgttcatcct gataattttc cggacgataa
     1141 aactctacac gattatggta gatatcataa agaccttcta gccctttacc cataccgatt
     1201 ggccaattca tcggataaga ttcgatatct aatagctctt ctaattcttc taacaattcc
     1261 aatggttcac gaccatcacg atctagtttg ttgataaaag taaatatcgg gatgccacgt
     1321 ttttttacaa cttggaataa tttctttgtc tgtgcttcga tcccttttgc actatcgatt
     1381 accatgaccg cactatccac ggccatcaaa gtacgataag tatcttcaga aaaatcttca
     1441 tgtcctggtg tatccaagat atttactcgt ttcccttgat aatcaaactg cataacagaa
     1501 ctagtaacag aaatcccacg ttgtttctcg atttccatcc agtctgattt ggcaaaatta
     1561 cctgtttttt tgccttttac ggtcccggct tgacggatag ccccaccaaa caatagtaat
     1621 tgttctgtaa tcgttgtttt cccagcatcc ggatgggaaa tgatcgcaaa agttctgcga
     1681 ttgtccactt gttgctttaa ttttggatta ttcataacct aactccattt cttccattca
     1741 attatttata ttttctctag gcacagattg tccgatctct taaagaaaga caatttcata
     1801 tagggctgtg acaaaagccg tgtagcagtc tctttttccg agtaaacggc gttctagaga
     1861 cagcctatca gcaataagcc cacccaagcc aaaaatacga aaagctgttt ttgcttggct
     1921 gctcttatta cttaatgcag gatagctttt tcacaacctt tgtcaactac tacaactgaa
     1981 tcagtatagc atggattcag cgaatgaaaa agccaccaaa ccataagagt caaatatttt
     2041 tttgtcagct gactaaaaga aaagacgtgt tgcttgttta ttcaagcaac acgtctttca
     2101 taaccaagtt tccaattatc ttcttggctc gtcatcttcg aattcttttc tgaagtatct
     2161 tcgttcttct tcaggttccg cttctgtttc ttccggatca tgaaaaatga cacggatttt
     2221 aaggattcga gaaccttcca tttcttcaga aaccagcgta atattatcga catcaaacga
     2281 taacttttcg ccttcatccg gaatcatgcc taatgctgta atcaaataac cagccatcgt
     2341 atctacatca ctcatatgca gatcactgtc aaaagcttca ttgaactcat caatcagcat
     2401 acgtccttgg atgatatatt cgtgttcatc gattttttca taaagattct caacttcatc
     2461 tgattcatca tcgatttctc cgacgatttc ttctaaaagg tcttctagtg ttgctaaccc
     2521 aacgacgccg ccatattcat ctaataaaat agccatctga ttctgcgtct ttttcatttc
     2581 atataacaga tcatcaataa agaccgtttc aggaacgaat aaaggctcct gcatgatttt
     2641 cttgatatct aaattatcaa agccgaattt atgggcagct tttagtaaat tctttgtatg
     2701 aaggattccg actactttat ctttgtcttc gttataaaca gggattcttg aatagttttc
     2761 ggataaaact tcattcacat tttcttccac agtgtcgttg atatcaatca taaatgcatc
     2821 cgttcgtggg accatgactt ctcgggcaac tttcgtatca agagaaaaaa ccccttggag
     2881 catttctaat tcttcattat tcaatacgcc ttcgttttca agcatataac gcatttcatc
     2941 tctggtcatt tttgaatctt catcatcaaa agtcatcggc gtgatacgcg ccaataaact
     3001 tgtggaagca gaaagcagcc agacgaaggg cttagctact acaccaatca agcgtatcag
     3061 ccctgaagtg aactttgcga cttcttctga tttatttaaa gcaatacgtt tagggtacaa
     3121 ttctccaaaa acaatcgata cataggtcaa aatagccagc acaatgatat ttgcaatatt
     3181 ttttgccgct gtgccgccac ctaataccgg agctaaccgt tccgataaag tattagctag
     3241 tgaagcacca gacaaaatgt tgaccaaagt gattcctact tggattgtcg ataggaaatt
     3301 atttggattc tgcaatattt ttaaaagctt ttgagatttc acatctcctt catcagcttt
     3361 ttgctcgatt cgattcttat tgatcgaaac aacagcaatt tcggaagctg ctaaaaaagc
     3421 attgatcaat gtcaaaacga tcaataataa aatctgcgct aacagcgact gactctcagg
     3481 gtcagcattc ataatcgcat tctctcctta taaaattaat ttttagttta cgatagaaaa
     3541 aaacgtggca ctatagccac gttaaaatgg tatcacaaag caattttctt tgcaataaaa
     3601 acaagtgtac taaaactacc tacgaggtcg tagcaatttt cttcttgttt tcattttgct
     3661 tgtcttcttt gacgattttg attatatagg aaatgatcgt cttgcagatt gcgtaaaaag
     3721 gaacacctaa gaagatacca agcaatccgg caatatttcc agctactaga agaacgataa
     3781 tgatcgttaa tggatggatt ttcagtgatt tcccaattac attaggataa atgatattcc
     3841 catccaactg ttggacgatt agcacaacca cacaacaaag taatgctttt accggatcat
     3901 tgaagactgt tactagaaat gcaggtgcta gtcctagata aggtcctaaa tatggaatta
     3961 ggtttgtcaa tcccgcaatg accccaaata agaaagcata atccacacca attaccatat
     4021 atcctaaaaa tgtgaaggta ccaacgaata aacattcgat tgcttgtccg ctaatgtaat
     4081 tagccaatgt tttattcagt tgccccaata actccacaat ttggtttctt cttttttctg
     4141 ggaaaaactg ttggatgttt ggcactagtt tttctccgtc tttcaacata tagaacaaga
     4201 tgaatggtgc tgtaatgatt actacggttg ccgaagcgat cgttccaacg atcgaaccta
     4261 aactattgga tactccacta aggaattgct gaatgattgt cccgtaagaa atgtctaatt
     4321 gctcaaaata tttattgata tctacttcct tgaacaaagg caattcggct acctctttta
     4381 accagctttc tacctgtttg atgaaatcag gcatattaga tgccaatgag gaaatttgct
     4441 gtactagact tgggatgaca ctaagtaaaa tccaaataat tgctgcaact aaaaggagca
     4501 gcacaattgc gacagcccat attcttttga tttttgcttt catcaacagc ttgacaatag
     4561 gattcaataa atagtaaaga aagcctgcaa tcagtacagg tgcaaataac gtggaaaaaa
     4621 atgtcccgat cggttcgaaa ataaagttta tttctgatga aatcatgatc aatgcagcta
     4681 tgatcaataa ttcaagcgac caaaacatta gtttagactg tctaagtttt tcaaacaata
     4741 gatttcctcc tttctttctt actagtatat attatattcg ttaaaaattc ttttattctt
     4801 tttttatttt tgaaaacttg ccatactttt tatttgtttt attttccagt atactatacg
     4861 aaaggaaggt ggctagacca tgaaagttgt acaaacaaaa gatacaatga gcaatattta
     4921 cttagatgct gtacggatca ggcatcaagt ttttgtagtc gaacagggtg tgcctttgtc
     4981 tcgagaaata gacaaagatg aagcccattg tatccacttc gtcctgtact cagataaaaa
     5041 agaaccgcaa ggaactgtac gtcttttgcc tttagaaaac ggaaaaatga aattacagcg
     5101 gatggcgatt ctttctgaat acagacacca agggttagga aaaatattga ttgaagaagc
     5161 agaaaacttt gctaagaatc aaggctataa tacgatttta ttaggggcac agtccacagc
     5221 tgaaactttt tacgaaaaac taggatatac agcttacggc gatccttttg aagatgctgg
     5281 catgcctcat atttatatga aaaagacttt ataaaaattt atttcgaaaa aagcaaaaaa
     5341 cagcaagctt ttgagaaaaa tctctcactg gcttactgtt cttttttgtg tttacttacg
     5401 cgacaattgc ttgtaacggt tataccaaat atcaatatag ccttccgaga acgggccatc
     5461 tccattattg atccagtcaa ccagaatctt cacattttct tttaggatga agtcaatatc
     5521 gttcgggtaa ttcatcattt tgctgtgcat ctcatattca tcgacatcca atagtcgttt
     5581 ctcgccgtca ggaaatacct tgatatccaa gtcatagtcg atatatttca aggcctcatc
     5641 gtccagcaaa aaaggtgaag ctagattaca ataataggaa accccctttt ctctgatcat
     5701 tgctattaca ttgaaccagt attttttatg aaaataaaca atcgctggtt cgcgcgttac
     5761 ccatcggcgt ccatcagatt cagtgactaa tgtgtgatca ttcacgccga tcatagagtg
     5821 ttcgcttgtt tttaatacca tggtatctcg ccacgttcga tgcaatttgc cgtcgtgctt
     5881 gtaactttgg atcgtcacaa actctccttc ttttggaact cccatagcaa cccttcctta
     5941 agcagcattt tgtgctttgt gtttgggtgt tcattacacc gacatcataa ttattatatc
     6001 aataaaaatg agctttgcct agaaaggatt cctattgttt cgatttttct ctaactgatc
     6061 taccagtttc atttggacct taggaaaaac gaccgaatca aatgcatcag gcttcaacca
     6121 cttcgaagtt ttgttctcgg taaattcttg ttctgctccc tcggttgccc gtccgtaaaa
     6181 aaggagaaca tgccatttca gatgactaaa aatatgagta acttctccta aatgtcgtgt
     6241 ctgccaaaca aagagttcct gcttttcaaa tgggagattt tgctcttctt ctgctactag
     6301 gtcatcaaac agatcgattt ctggctttgt ttcccattct tttttcaatc gctcatactc
     6361 ctcttgtgtc acttccatca ttggaaatgt ccacatattc gccagcaatt tttggctatc
     6421 tctttcttca aaataatacg ctccagaatg attttgaaga gctgcactga tataataaac
     6481 gtcttttggc tttgcttttt ttgtttttac aggaaaactc gtttggattc ctcgtttgtt
     6541 agctaaacaa aaggcttgga tcggacaagt ctcacacttt ggagaagttg gtgtacagat
     6601 agctgaacct agatccatca ttgcttggtt gaattcacct ggatgctttt catcaatgat
     6661 tttccgcatc gcttcatcaa agattttcct gctagaagct tttgcgatgt ctgcttcaat
     6721 gcaaaacaat ctgctgacta ctcgcatcac gttgccgtca actgcaggtt ctggaagtcc
     6781 aaatgcgata cttgcaatcg cgcctgtcgt atatggtcct attcccttca atgagctaat
     6841 ttcttcaggc gtttgcggca tttccccatc aaattctgac atgatctgtt tagcagcagc
     6901 ttggatgttc cgggctcttg agtaataacc aaggccctcc catgctttta aaagcttttc
     6961 ttctggagca tttgctagct cttcaattgt aggaaaccat tccatgaaac gataaaaata
     7021 atcgatcact gtgtctactc tggtttgctg aagcatgatc tcagagatcc agatacgata
     7081 aggatcacga ttatagcgcc aaggcaggtt tcttttttct tgttcatacc attgaatgaa
     7141 ctgatcttga aattcttttg tttctttatc tgtccatttt tcccattctt tattcacttt
     7201 cctcttcctt ttccgtaatg aatcgctgga tcatatccaa atcaaatcct ttttgatata
     7261 agctttgctt tacttttaga tttctttttt taggttcaaa ccttaaattt cttcgccata
     7321 gtttatctcc ttgtttgact aaataatcat attctatttc ttgatctgct tcttgcggaa
     7381 gatcagctat cgcttgttgg atcacttctt gagaaaagcc tttcgtcatc aatgtttgtt
     7441 ggatttttct caataattct ttttggcttt ttccgtgatt ttttccaaac gctttttcgg
     7501 ctgtctgatg tgcattttga acttggtctt caaatgtata ttggctgagc gcttgttcaa
     7561 tgatttcagg tttcaaacct ttttgctgca tctgctgcgc taatttcttt ggtcctttgt
     7621 cacttaagcg catattcgtg cgcacataac tttccccata gaccaaatca tcgacaaggt
     7681 ccaattcttt taagcgggct acgattttat ttcgatcttc ttgaaggatt tccttttctt
     7741 ttaagtatgt tcggatttct ttttccgatc gcaactgata acttaagtaa ttcattgcta
     7801 ggtgaaaacc gaaatcagct ccgctactct tcttgatctc ttggatagtt tcttcgtcta
     7861 attcccgtcc ttttaataat cgatagcgta ctaatacatc ttcagatact tgtaaagctt
     7921 ctccttcaga taaatctacg cgatagaaag ggcctttgcc tttactgatt ctcgtaacgg
     7981 ttaacataaa catcctcctc gaacatatat tctctatcat accacagaaa atccccgtaa
     8041 tcaggtatta tatgttagaa taaaccacag aaaaaagaaa aagggagata cttatggcag
     8101 aaacaattgt caaaactgga caaacggttc ctttgaaaat caagcgttta ggcataaatg
     8161 gtgaaggaat cggttatttc aaacggttga tcatatttgt tccatatgca ctaccaaaag
     8221 aagaagtact agtgaaaatc acgaaagcaa ctcctcgcta tgcagaagga gaattaataa
     8281 aagtcaaaaa aacaagcaaa gatcgcgtag tggcaccatg tcctgtttac tatgaatgcg
     8341 gtggctgtca attacaacac ttagcttatc aagcgcaatt ggattttaaa aaggatctgc
     8401 ttttgcaagc attggaaaaa ttcaaaccag caggctttcg atcttatcaa cttttgccga
     8461 cgatcgggat ggatgagcct tggcattata gaaacaaagc acaatttcag ttacgaaaaa
     8521 ataagcagac tcaaaaaatt gaagctggtt tatacgaagc aaactctcat cagttagtcc
     8581 ctataacaga ctgcttggtt caagaacctc gaacacaaaa agtaatgaat actgttgttc
     8641 aactattgaa taaatttgat gcacccatct atgatgaacg gacaaacagt ggaatcttcc
     8701 gcacaatcat ggttagaatc ggtgttaaaa ctggggagtt acaagttgtt tttattactc
     8761 gcagtaaaaa attcccgcaa aaaaacgcaa tgatccgtga gatcaatgaa cgactgcccg
     8821 aagttgtttc aatcatgcaa aatgtccagg cacaaaaaac gtctctcgtg atgggggatg
     8881 aaacgatcca cttatgggga aaagaagcca ttgaagaaca aatcaatgaa gtgactttcg
     8941 atctttctcc tcgtgccttc tttcaactca atcctcagca gaccgaattg ctttatggcg
     9001 aaggaatcaa agcattagat gtccagccta atgaaacgat cgtggatgct tattgcggag
     9061 ttgggacgat cggcttaagt gtagcaaaac aagccaaaga agtacgggga atggataccg
     9121 ttcctcaagc aatcgctgat gcaaaaaaaa atgcggaaag attaggatat cacaacactc
     9181 gatatgaagt cgggacagca gagcaagttc tgcctaaatg gttgaatgaa ggttttcagc
     9241 ctgatggtat catcgtagat ccaccacgta cgggtttgga taacgcattg atccaatcat
     9301 tattgaaata ccctccaaaa aaacttgttt atatctcctg taatgtttct acacttgcca
     9361 gagatctggt taatttaagt aaaaagttca agattgaata tttacagtcc gtcgacatgt
     9421 ttcctcagac tgcacgttgt gaagttgtcg tgaagatgac gagactcgct gactaaatga
     9481 aagaggttgt gaaaaagtct gccctgtatc aagtaataag aacagccaaa caaaaatagc
     9541 tcttcatgtt ttatgaatac tgtacttgtg accgaagagt taacaggagg acactattta
     9601 tttaaaaaaa gggttgtgac atagtttttg tcacagccct ttttgattat tattggatat
     9661 tcacgtctat cacttgataa aatgcatttg tcgtatcgct gatattccat actgctaaaa
     9721 tcacattata tccttttcga tcctgcggaa gtgtgatcgt ttggttgact gtttttggtg
     9781 gaacagttgc tttgtcgtct atttttgtga tcaattcaaa atcactaaat ttcaaaggtt
     9841 gattaggatt ccaagttggt ttggtaatgt agtaatccca agtacttgtc cgatgttgtg
     9901 ctgttaaatt ccaagtaata tttaatgttc ctggattaac catgttttta tgccatctgt
     9961 tagatgtctg ttcatctaaa ggctcaaagc cgctgactcc tgcactagca atttttccat
    10021 caatgaatgt atttttggta gcttcgatac tttgcggttc ccattgtgct cttccgacat
    10081 tttgatttaa gtttcctgct gctgatgttc ctaggtatgc acggcttcct ggagaagtaa
    10141 tataaccatg cgcaaaacct ggaatgctaa aaccgaacaa ccctaaacta actaacccaa
    10201 taaacaataa cgtactaatc tttttcatgt agaaacctcc ttcatttgat aaccacattg
    10261 taaacgcttt attgtattaa cacaaacatt atgcttttat aattttatgt tgaagtaaaa
    10321 aaggtgaaaa aagtaagatg cagattataa cagaagtaga cgagaaaagt acaaatagaa
    10381 aacatgagaa tcaaatcata tgatcttttt caaaaacatc attcatataa acaaatgttc
    10441 aactttttaa taacaacaat tatcaatttt tttcttactt cacaaactaa atacaggtct
    10501 tgctgtgagc cttcatggta aactactatt atagaaagga gttagtaaaa tgaagatgat
    10561 aaaagatatt cgtttttggg tttgtgttat aggaattgtc atattaggct ttttatcagg
    10621 gttgcttagc ggaaatcccg gagaatatta ctattctttg caacttccac catttgcacc
    10681 acctagctgg atatttgggc cgatgtggac cttactgtat atattgatgg gtatttctct
    10741 ttatttgtta cttaatcaca acaacaaaaa acagcgaaac aatcttgtcg gtctttttgt
    10801 gattcagttc atttttaatt ttatatggtc tgctctattt tttaatttaa gaaatatttt
    10861 cattgcagca atcgatatta cacttttagt tattttcttg tcagtgctta tgtatcagtt
    10921 gtggctacat catcgcctgg caatgtggct catgattcct tattatttat gggtactatt
    10981 cgcaactcta ttgaattatt caatttattt tttaaactaa gcgctaaaaa gagacgtcct
    11041 gtaatttaac acatagaaaa agtgccacct aagttagatt ttttgtcta
//
LOCUS       DACEMS010000093        10938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEMS010000093 DACEMS010000000
VERSION     DACEMS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.096"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            80..1339
                     /gene="lysA"
                     /locus_tag="IW735_002074"
     CDS             80..1339
                     /gene="lysA"
                     /locus_tag="IW735_002074"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1045065.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1326..2126
                     /gene="dapF"
                     /locus_tag="IW735_002075"
     CDS             1326..2126
                     /gene="dapF"
                     /locus_tag="IW735_002075"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1045066.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2129..3010
                     /locus_tag="IW735_002076"
     CDS             2129..3010
                     /locus_tag="IW735_002076"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1045067.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3007..3723
                     /locus_tag="IW735_002077"
     CDS             3007..3723
                     /locus_tag="IW735_002077"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1045068.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3729..4424
                     /gene="dapD"
                     /locus_tag="IW735_002078"
     CDS             3729..4424
                     /gene="dapD"
                     /locus_tag="IW735_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1045069.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4440..5441
                     /locus_tag="IW735_002079"
     CDS             4440..5441
                     /locus_tag="IW735_002079"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1045070.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5438..6799
                     /locus_tag="IW735_002080"
     CDS             5438..6799
                     /locus_tag="IW735_002080"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1045071.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6759..7910
                     /locus_tag="IW735_002081"
     CDS             6759..7910
                     /locus_tag="IW735_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1045072.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7885..9054
                     /locus_tag="IW735_002082"
     CDS             7885..9054
                     /locus_tag="IW735_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1045073.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9229..10608
                     /locus_tag="IW735_002083"
     CDS             9229..10608
                     /locus_tag="IW735_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1045074.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3632 a         1912 c         2454 g         2940 t
ORIGIN      
        1 attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac ggaaaaaaag
       61 tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag caagcacatt
      121 ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat
      181 gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc tttacgcctc
      241 taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat tcgggctaga
      301 tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta gagatactat
      361 ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg aaaatgattt
      421 gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat taagccaacg
      481 ctatttaaaa aaaatttgta tcatgcttcg gttgaatgtc ggagtagaag cacatactca
      541 tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg aaagtaaaga
      601 gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag aaggatttca
      661 tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt
      721 gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg gagggtttgg
      781 gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa aaaaactgat
      841 tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat tactgatcga
      901 accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg gtttcatcaa
      961 aaagacacca cataaaaaat attactttgt agatggcggg atgacagata atatccgtcc
     1021 agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg aagaaaaaac
     1081 agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc
     1141 ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg gagcttatgg
     1201 atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt ttgtcaataa
     1261 tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa ggggggaagt
     1321 ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta ttagattacg
     1381 cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa aaatatgata
     1441 tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac aacaaagatg
     1501 gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc
     1561 agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca agaaaaatta
     1621 cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca gatgattctc
     1681 cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata gagatcaatg
     1741 gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt gtttttgttg
     1801 aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat catcctttgt
     1861 tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc
     1921 gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt
     1981 atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt cacttggaac
     2041 aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca gcagttcatg
     2101 aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac
     2161 gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga tccaattcca
     2221 tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat cttctacgct
     2281 cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg ctggccgtgt
     2341 gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa aagcgaagca
     2401 ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca acaaaacaag
     2461 tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga ttccgcttat
     2521 tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat tagtaaattt
     2581 agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg cttatgtgat
     2641 ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg atgatttgat
     2701 cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga atatccaacc
     2761 taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc aagcaaaaga
     2821 aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca atcctattcc
     2881 tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc tgcctttagt
     2941 ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag aaaggacggt
     3001 tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt cgaagaagtt
     3061 gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga agcaactacg
     3121 cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga taatttagag
     3181 aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac gacaggttat
     3241 acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt gatgtatagt
     3301 gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc agctgcaatg
     3361 ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa agatgcacca
     3421 tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct gcagcccgta
     3481 tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag tattcgtgca
     3541 ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat atttacgatc
     3601 aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc agcggactgg
     3661 ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga caaaggggtg
     3721 taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct aaaaagacgc
     3781 ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt aaagtttttg
     3841 gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg gttttagaag
     3901 ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc
     3961 ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt atcagagatc
     4021 aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat attggtgcag
     4081 taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga gcgacagtgg
     4141 gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg
     4201 ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg attgttgaag
     4261 gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg
     4321 cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt gatgatcaga
     4381 caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga tggagaaaaa
     4441 tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa acgatgctca
     4501 aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct tcaaaacgtt
     4561 cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa
     4621 cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta tccaaagagt
     4681 ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca gcatggcggg
     4741 aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat acattgaatc
     4801 ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt aaagctttac
     4861 aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt tctggttccg
     4921 ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca gttttgtatc
     4981 cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa aatgggtata
     5041 caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag cctgctttgc
     5101 cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag
     5161 ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc cagtttccag
     5221 ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt cttgctagag
     5281 gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag aacggattgt
     5341 tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc gtacagattg
     5401 ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc tgaaagtagc
     5461 gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc gtacaattgt
     5521 tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga
     5581 tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc atcatgttga
     5641 ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag atgttcttgc
     5701 cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg aagacgaaaa
     5761 gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac tgatggctga
     5821 gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact atgatgggaa
     5881 aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat atccgaaaat
     5941 ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg
     6001 gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa gttatgagaa
     6061 ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc atcctaaaat
     6121 catcgaggaa atctctttta gagaactgag agagatggct tatatggggg ctaacgttct
     6181 tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa taaaaaatac
     6241 caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg
     6301 actgacagga atagctggac gcaaggattt tctttccatc actttattca aacgtcatat
     6361 gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac atgagatatc
     6421 aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag cagaagcgat
     6481 tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg tggaagaaat
     6541 cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata aagaattgat
     6601 tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca catcgatctt
     6661 atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc aatatgaaac
     6721 agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa tatcgaatgg
     6781 cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa tatttatatg
     6841 aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga ctactccttt
     6901 attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat gcactgccag
     6961 tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg catgcatgtg
     7021 gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact aaacacattg
     7081 aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt tccggtggcg
     7141 cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca atatttggct
     7201 ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga cctttgatgg
     7261 cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt gcttctagtg
     7321 aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt tatgattttg
     7381 accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc caaggtggaa
     7441 cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt tcttacagtt
     7501 cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag tttcactcta
     7561 cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac catagaggct
     7621 tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca gttcttcaag
     7681 ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt ttaggtgtcg
     7741 gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt ttagaaaatg
     7801 gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa atcgatagca
     7861 gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag tcctaggctt
     7921 tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc gataccagat
     7981 gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt caagcaagca
     8041 ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg attagaggaa
     8101 ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta tgatccgcaa
     8161 acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt
     8221 ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata tcagccatta
     8281 gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg gtttgtctgt
     8341 cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa agcagtcatt
     8401 ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt gaaagcaatc
     8461 gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata tgctgaattg
     8521 acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac aatcgtgata
     8581 agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat ttttgctcaa
     8641 aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc cgctacaacg
     8701 atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag cgagaatatg
     8761 aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc tttaggattt
     8821 gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc tgccattcaa
     8881 actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc gtgtatccca
     8941 ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc tagcagcatg
     9001 gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca gtgattcata
     9061 gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag acgattgttt
     9121 tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta atgttaagtt
     9181 taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat ggaaaaaatc
     9241 aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga actattgaca
     9301 cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat ttccttcatg
     9361 tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga tgtacgtaat
     9421 ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca cgaagtaaaa
     9481 acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa cgaaacaaaa
     9541 gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa tctgccagta
     9601 ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc aaccaaaatc
     9661 aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc gggttacatt
     9721 gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt gcttgatgtg
     9781 atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt agaacaacac
     9841 ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt tactggtaac
     9901 ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct agtcattcaa
     9961 gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt agatgaacgt
    10021 ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta tgcagtagga
    10081 gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc tttagcgact
    10141 gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt accaaacaaa
    10201 ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca ttttgcagca
    10261 agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt gtcttattat
    10321 gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa agtgtccatc
    10381 caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt atctacctat
    10441 gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact gactttagaa
    10501 gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg gagcatgttg
    10561 aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga aaacgaaata
    10621 tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagtaaaa
    10681 acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca
    10741 ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc aaaagttaga
    10801 ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact tttgaactta
    10861 aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta aaaatccaga
    10921 tcaagagatt gaagcgga
//
LOCUS       DACEMS010000094        10929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEMS010000094 DACEMS010000000
VERSION     DACEMS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.097"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..407
                     /locus_tag="IW735_002084"
                     /pseudo
     CDS             1..407
                     /locus_tag="IW735_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(309..1025)
                     /locus_tag="IW735_002085"
     CDS             complement(309..1025)
                     /locus_tag="IW735_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1045075.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1149..1334)
                     /locus_tag="IW735_002086"
     CDS             complement(1149..1334)
                     /locus_tag="IW735_002086"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045076.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1561..2834
                     /locus_tag="IW735_002087"
                     /pseudo
     CDS             1561..2834
                     /locus_tag="IW735_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2941..3972)
                     /locus_tag="IW735_002088"
     CDS             complement(2941..3972)
                     /locus_tag="IW735_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1045077.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3979..4809)
                     /locus_tag="IW735_002089"
     CDS             complement(3979..4809)
                     /locus_tag="IW735_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1045078.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4806..5612)
                     /locus_tag="IW735_002090"
     CDS             complement(4806..5612)
                     /locus_tag="IW735_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1045079.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5618..6442)
                     /locus_tag="IW735_002091"
     CDS             complement(5618..6442)
                     /locus_tag="IW735_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1045080.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6432..7532)
                     /locus_tag="IW735_002092"
     CDS             complement(6432..7532)
                     /locus_tag="IW735_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1045081.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7710..8495
                     /locus_tag="IW735_002093"
     CDS             7710..8495
                     /locus_tag="IW735_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1045082.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8528..8911
                     /locus_tag="IW735_002094"
     CDS             8528..8911
                     /locus_tag="IW735_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1045083.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8987..9118
                     /locus_tag="IW735_002095"
     CDS             8987..9118
                     /locus_tag="IW735_002095"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1045084.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9087..9323
                     /locus_tag="IW735_002096"
     CDS             9087..9323
                     /locus_tag="IW735_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1045085.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9401..10372)
                     /locus_tag="IW735_002097"
     CDS             complement(9401..10372)
                     /locus_tag="IW735_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1045086.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3249 a         2541 c         2088 g         3051 t
ORIGIN      
        1 agattggatg actcaccttc ccattgtgaa cactgtggat cgattcgtat tatccgcaac
       61 ggttcctata cgacacgtac tcagatgttg aaagtcaaag aaaagttgac tattttagaa
      121 ttaaaacgta cccgttttct atgctatgat tgtgggcaga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcgattct catagaactg
      241 tacgaaaatc aatcacgtaa tctgaaaatc agaagaagta ccgtcggctc aagcgatatt
      301 ggaaactctt actcaaagat tccacgacat tggagccgct caaacggcat tatcatcgac
      361 tgtttaaacg accaattagt caaacagaaa tcgtcgatga accgttgtgc acaatggtgt
      421 cctgaacgta cagcgacacc ttccatatcc agagcagtcg ccacatcacg gggatggatg
      481 ccatccaaat taaaagcaac cacgccagta tgatgttcag gatcttgcgg tccgtaaatg
      541 gtgataccgc cgatcgcttg taatttaggt aaaacgtagg caaccagttc ttgctcataa
      601 cgatgaatat tctccatacc aatctcattc aagtaatcga ttgctcctgc aatgttcgga
      661 gtaccagctt caaatttcca tggcagctct ttccatgaac tatcgtaaag attgacaaaa
      721 tctatcattt caccgccgaa ttcgactggc tccatttgtt ctaacaattg acgtttgcca
      781 tataatacac cgattcctgt cggcccgcac attttatgcc cgctaaaggc gaaaaaatct
      841 gcatccaacg cttgtacatc aactgtcata tgcggtgctg cttgggcacc gactggacta
      901 tcctcaacta caacagcctg ttcaaccctc agccgtttca agcacaacaa ataaccttct
      961 ggatctggtt tgccgacctg aagatcatcg atcgtcacaa aggaatcaat tttttcctta
     1021 atcatcggat gtgcttcaaa aaaatagtct agctcccgca gttcagaatt gagactactc
     1081 cgattttcca gtgctgctga tccgctgctc aaaatgttca gcatccactg gtctcccaac
     1141 cgcttgactt atcccttcat aaaaggacag ttctgctgcc gtcccaacac cttgttgata
     1201 gtctgcaaaa ggaattttat agccgaactg ctccagtaaa taggcttcag taaatagtga
     1261 tgaatactct cgtaaaacat ccattcactg tcaatcatcg tcccatcaaa atcaaaaata
     1321 agtgctttgg tcatcttctt tcccccttac ccaaaacaag caaaagctgt gtcctgctct
     1381 ctctacagta tcagcataaa aactgccgtc tgaaaagtca aaggtgaaag aaatgtggcg
     1441 ctataatttc taggactgat agtaaatcat tctatcagtc cttttgagca taaaaaaaca
     1501 aaacatacag aagctccgtt agaattgaat caccacaaca caatcaaagg agcgttctgt
     1561 atgcctgatc ttcatcttat caaactatta ctaggaatca aagatggaaa cattcaaatc
     1621 actcatgtcg agaagaaaaa aatacattca atcaacagta tcgttgtttc tgccacgctc
     1681 attaaaacga ttcagccctg tcctcagtgt aaacaggtga atcaacatgg gctgatcgtt
     1741 aagaacggaa agaaaaaatc gctggtcctt ctcaataagt gtgcgaatca acggacttat
     1801 cttgctttgg caaagcaacg gtacctctgt cgtggctgtc acacctattt tacggcgaat
     1861 acctacattg ttgaaaaaaa ctgttttatc gccaaacaag tacattataa aatattggag
     1921 gaattgactg aaaaacaagc aatgacgacg atttctaaac attgcggcgt ttcttggtca
     1981 accgtttcgc gaacactcgc ttctctgctc cccatgacaa acgtgaaaat aaattggttg
     2041 cctcgctgtt cattggtaga tgaatttcgt tcattgaaaa atcaagtcgg tccctattct
     2101 ttttcttgta tggatggcga tactgggaaa ttactggata ttcttccttc acgtaagaag
     2161 aaagatctca tttcttattt tatgcaattt gaacgacgtg cacgtttaaa cgtaaaaata
     2221 ctcgtaacag atatgaacgc ctcttatgct ttgctgataa aagagtgctt tcctcatgcc
     2281 aaactcgttg tggatcgctt tcacatcgtc aagcacctca ttcgcagttt taaggatatt
     2341 tgactatgag tcatgaaaag cttcgaccga aacgatccaa tacaagccaa acactatcga
     2401 caagtgaagg ctttatctcg acttctgatc aaacgacagg acacgcttgt ttatgataaa
     2461 tggacaaaat ggcgtaattt tggttgggcc tatttaaccg aaagtgaagt cgtagaacgt
     2521 ctactttcta cttctgatga actacgaata gcttatgctt actatcaaga aattcttcaa
     2581 gctttttatg acaaagaagc ggacatcttc tttcagcttg taaaaacaat gcccaagtcg
     2641 gttccaagag aactgcatca tataaaaaaa gcgtttataa actatgaatc cggcattcgt
     2701 ttagccttag agttgcctta ctcaaatggc aaaatagaaa atctgcatac ccacatcaaa
     2761 gcattgaaac gaatagctta cgggttagga tgagaaccag aattttctta atcaatcatc
     2821 tgatcactta ttaaacataa aaaaacatct aaagcagaaa atcaattcta ccttagatgt
     2881 tcttatatca ctctatcagt cctatttgac aaagagccag aaatgtttgc cgactataca
     2941 ctagctttgt tgtgcaaaat gctgagtatc aatttccaca gggattgcta gaatatcttt
     3001 cagctgaatg tataaggcat cgctaatttt ctcttcccga tcgttcaacc gatccactaa
     3061 aatttctttg ccctccagcg tttgaaaaac ataccgttga atgttgccta aaataaatga
     3121 ttccttccat agaaccggaa tcttaacccc ctgaggaacc ggttcaaatt gaatcagctc
     3181 cggtcttaaa taataaatgc agcgattgcc cttagcggaa aactcgtggg tatactgatt
     3241 cagctcatac cattcgaaac gattatagtt gccgataaat tccgcaacaa atcggctctg
     3301 aggattctga tacaattcct tcgaggtgga aacctgagcc acccgcccag cttccatcac
     3361 aaaaatccga tcactgattc gcatcgcctc ttcttggtca tgagtcacaa aaatcatcgt
     3421 catccctagc gattgctgga tagcgcgcaa atcccgctgc agctgttttc taattcgggc
     3481 atccaaagca cttaatggct catccagcag caggactttc ggttcagtga ccaatgagcg
     3541 agccagtgcc acccgttgtc gttgaccgcc agaaagaaac tggacatttt tatctgcctg
     3601 ctccgtcata tccaccaatg atagcatctc aaaaaccttt tcctgaatca cctcaggact
     3661 cagcttcttt accttcaaac caaaggctac attttcaaaa acagtcatag ttggaaacaa
     3721 tgcatactgt tgaaaaacaa agccgatatt gcgatccttc gtaggcttat cggcaatatt
     3781 ctcattgtcc agcacaatct caccgctctt tggttgttcc aatcccgtga tacagcgcag
     3841 caacgtagac tttccgcacc cgctgggacc tagtaaagta accagctcac ccttttcaat
     3901 ctgaatttca tcaatagcta aaatctgttt ttcatcaaaa aacagattga gatcatgtat
     3961 ttctaaaaac attttccgtc acaactcctt taagggcttc tttttcgact gcttctcttc
     4021 cctcgtctgc ttaagggaca gctgttgctt ttgtcgataa gtcagcataa aggtagttaa
     4081 agcaattccc accataaaca gcaaatacaa aaccatcaca gcgctagcca aatggccgtt
     4141 ttcgttcatc cgccgcagca tatagatgcg taccgtctga aatcgaccgc caatcaacag
     4201 gttcgtcagc atgtactccc caaaaaccga agaaaaaatc atcagagaaa ctagggtcac
     4261 accgatacgg atgtttggaa acagcacctg gataataatc gtagacatcg gggcgcccaa
     4321 ggtttctgct gcatccacta attccttcag attaattaag cgcatctggt tgttaagact
     4381 aagatagaca atcggcaacg ccgtaataaa caatgcacct accaacacta caaacattgg
     4441 gataccagtt tcagcatacg ttcgaagcaa agccgttacc aaaatcactc ctggtaaagc
     4501 atacggcagc aggagcagct tttccagcca acgattcaaa cgagggaaat acaaatgaat
     4561 ccaaataatg gtgggaatca tcaacaacag aatcgtcacc aagaccacac ctgctaataa
     4621 aagtgagcga cccaccgcag caataaattc cctgtccgtc agcagctgtt ggaaccattg
     4681 caaggtaaaa tcactaggta aaagcgattt tgaccattta gtggagctgg catacaaaat
     4741 cgttccaata atcggcagca agtaatacag gacaaagcag aacaaaatcg gttttattaa
     4801 ctttttcacg tctgcaccac cttgcctttc ttttgaataa tcaacattgc cgttaccagc
     4861 aggacaccta aagcgactgc caacgcactg cctaaattag gcttggcgaa cacatctccc
     4921 gacactaaag ccgcaattcg aatcgttaaa agatttacat tactgctggt cagggcatac
     4981 gctgtttcat aggtccccat gccattagca aataagatga caaaggtgct ggcaatcgat
     5041 ggtcgcaaaa aaggcaaagc caccttgaac caagcatacg ctgatgaagc gcccagcaat
     5101 tgtgcggact ccagccattc ctttttcact tccttcatgc tgggatacaa aaacaagacc
     5161 cctaacggaa tttggaaata ggtatacgtg atggtcaatc cccaccaaga atacaaattg
     5221 aagtccgcta gaaatggcaa aatcattttc agcacaccta cattccccaa caaaataata
     5281 aaagagaatg ccagcggaat cccagcaaaa ttagctgcca aattggaaac aaaggtgatt
     5341 ttctcctgca atttagtcga tagattcatt aagcataaac ataaaataaa cgtcccgatt
     5401 aacccaatca cactggagct gacagcaatc agtacgctgt taacaaaggc ctgtgtgtaa
     5461 tatttgttgc tgaaaatatc actgtaattg gccaatgtcc actggttagt taaatcactg
     5521 tgcaagctgc acagaaccat aaaaagcagc ggcagcagta aaaatgcaca gaagataaag
     5581 gaggcaggaa ccagaaccaa gcgttttttc atctctttca tcccctcatc aattcttgaa
     5641 tgtcctgcat tttctctgac ggtgcaattc ctaataacca gcaaaacaat ggagccgcca
     5701 gcagctgtgg tacagtttgt tccttcagct tttggcgttt atttgaaatc aaataaaaag
     5761 gaacttcacg atgtgcagcc aagctgccgc catgcagtcc gtgcgcatcc atcccgtgat
     5821 ccgcagtcac cacgacttga taaccttttt gcagccattc aggaaggtaa atagccaata
     5881 atctgtccgc ctgattaacc gctgctgcat attcatgaga atcggcggta aatttgtgac
     5941 cgacatcatc aatattcatc gaatgtacga ccaaaaagtc tggctgatac tgattcacta
     6001 aataatgggc atccgcaaat aaatgcgaat cagggtaagt gtcctcacta taaaagatgc
     6061 catgctgaat cagtcgctcc tcctgatgct ggatgcgatc aaccaaacga ttataggggg
     6121 cttgattgta caactcgctg taccagtggt aagcggcggc tcctgttttt ccgccagctt
     6181 ccttaactaa ttcaaacagc gaccactctt tggatcgctg agcatggtaa ttactataga
     6241 tgccattttc ataggctgga acgccggtca tcagtacttc gtataacgga cgtgaattgc
     6301 tgggcagctc actttttacc ttcaataagc ttgcttgctg gtgctcaact aaatgattca
     6361 tgaacccaag ctgggcgacg gctgtgtcgt agcgacagcc gtctagtata actacaatca
     6421 gtttgtcaag tctactttgc ataggtcagt acctccgcct gccaaagctc tggaagctct
     6481 acagaggtct tgttccaagc ttcattgtct tcaactggca ccgccgcttc ataagcatcc
     6541 tccggcagta atttttcagc tacttcttta ggcagctcta ccttgcgaat aggtcgcgca
     6601 tagccctttg ccaaattgat ttgcccttca tcagataaaa tatattcccg tgctagcttt
     6661 gccgcattgg gatgaggggc atttttattg attaaggttg tatacccgct aatgatggag
     6721 ccgtctgttg gcacagttac ctcaaaacgc tcggagtcaa tttggtcacg ataatttagt
     6781 ccattgaagt cccaaacaac tgccacatcg atttcacctt tttcaagatt tgctacgctg
     6841 gcatcaacgg tggacaaacg gcctttttct gccatttttt tgtagtaatt cagacctggc
     6901 tgaatatcct tttcgtcacc tccgttggca atcgccgcag ccaatacagc aaattgtgct
     6961 tggttggcaa tcgtcacatc ccccacgcta acttgatagt caccctcttg cagctccttc
     7021 cagctagtcg gtgcttgctt cacattctct ttatcggtga taaaggaaat ggttcccgta
     7081 tagcttaaca accaatcccc atcatcatcc tttgcccaat ccggaatttc ctcccaatag
     7141 ttggtcttat aaggtaaggt taactctttg ctttcagcca acggaccgaa gctgataccg
     7201 acatcaccaa tatcggcggt tccatttttt ccttctgatt caaacttagc caattcttct
     7261 gcactggaca tatccgtatc cacgtgactt aagccgtatt ctttttcaag gtcgttccaa
     7321 gtgcctaccc aatttgccca agtatccggc attcccaagc tggcaatctc tccttcttcc
     7381 ttggcttttt ctataatcgt atctaaagag tcgtctgccg cagccgattg cttcgttgct
     7441 tcacctgaga cgcagcctgt cagcaggata cttactgttg ctgcacaaaa taaaccgata
     7501 gtcatgttac gaattttttc attcattttc attcattctt ctcccctttc gataattcca
     7561 ttctaacaaa gctttgtaat gagagaatca gtttcttgaa aaggtttcat gaacaatgta
     7621 aaggttgtat atggtttgta aatttcgacg aattcccgtt aaccttctaa agcttttgcg
     7681 gtatgatagt agaaaaaagg aggctcccta tgcagtatta tgatcagcat ctccacacgt
     7741 atttttcctt tgattcagag gaaaaatttg aaaactattt agcctatcag ccggagtttt
     7801 tcgtttctac ggatcacttt gatttaaaca atccatctac cggaaaagat gacattccgg
     7861 attatccagc ctatataaac aaactggact ctttaaaaga aacctattcc accaaatttc
     7921 tgcgaggtat cgagattggc gttgttccag gacaagaaga gcggatcgtg gattacctcg
     7981 ctcagcatcc ttatgattta aagctggtaa gtattcatca aaatggccgc tttgattata
     8041 tggacgatgt ggttttaacc aaagatccca ttcaaactac caaggaatat tttgatcaga
     8101 tgttgcaggt cttgacggat tttccagaag gagatatttt gactcacttt gattatggat
     8161 tacgccgctt caccttcaca gcggaagaat tcgcggagca ttttgaggct cagctgctaa
     8221 cgattttcaa aaaggttgtt gaacgagaat tggcgatgga gttaaatgcc aagagcttct
     8281 tgaaatacgg caacgaagac ctttaccgtt atgccgttcc tttgtatcaa agtgtcggcg
     8341 gaaagctctt taccttaggc tcggatgctc atgttgccga agattatcag ctgggcttta
     8401 aaaaaatgag tcagctgttg gcagactgtc aggtagaaaa gttgctagtc attcaaggaa
     8461 aagaacgccg cctgacgccg ctgccgattt tgtgattcct atataataga agaaagtggt
     8521 gaaaaaaatg agcaacctgt gggactacaa tcaagaggca cccatccatt atttaattgc
     8581 gcgacattgg gatgcgttga aaattgaggc ggtttgccga tccttgttag cagccgttcc
     8641 aaaacaacaa ctggaaaatt ttttggtggc tgacagcttg cagcgggaaa aagttcaggc
     8701 ttattttgca gcctttaaag atcagccttt ggaatatttg catgcgcagt ttcatctttt
     8761 ttatcaagta gccgccccag atgactacaa cgatttacga ggacagctgc aattgacctt
     8821 tcaagcggat gaaaccgcct ataccgtttt attgggaatg gctcgccttg gtgatcaggc
     8881 gaaggtcgag tggcggattt tcgatatttg atggcaggat caaggcgttt cagttgcaaa
     8941 ccttcctctc tttcaattag aaaaaccgca gcaggaagaa gtttgtgtgc tggtcaccga
     9001 tgctggtgaa attcgtctcc gcttatttcc tgaccaagct cccctggctg tagccaactg
     9061 gcgctcccta gctgagcaag gcgtttatga caacacacct ttcggctcgt gtcattaagg
     9121 aatttgttat tcaaggtggc gctttggatg gttctggtga tgaagctgtt tccagctatg
     9181 agggcttttt cgctgatgaa gtgcatcgcg gactttatca ttttaatggc gccttggcct
     9241 taggggacca cggcccccac accaacggca accagttttt tattgtgcaa aacacaaagg
     9301 cgcaggctga cctgctaatg taaagcaagc atacttggag cttggaggac taccggagct
     9361 ggatggtcgt tatactgttt ttggacaggt ataccaaggg ttagaaatcg tagcagcgat
     9421 cgcagctcag ccgacaaaca gtgaagatgt accgattgag ccagtgttca ttcgacaaat
     9481 taacttcgaa aaaacatcgt aaaaagacac tcactgactt cagcaaaagc tctattcttg
     9541 agctggctgc agctagtgag tgtctttcat ttatccataa gataaagctt ggattcaata
     9601 aataaataaa tgttttggtt aaagctgtga ccagcaaatg aggaaccgtg cgagccagtt
     9661 gatctgccaa tgcaaacatt tctttaggtg aaggcgctgg cgtgtctgcc aacggatact
     9721 ccagtcccaa tgcttggtaa ccgtgcatac catagcagtg gtacggtagt aattctaacg
     9781 gtacgaaccg atcaagatct gccacaaaac aagcaatcgt cagcagctcg gtcaggttca
     9841 tattgatacc tggaaccaca ggaactcgaa ttcgcacttc gcagttagga tactcaatta
     9901 aaaagcgacg aatattcttc aaaatttgtt ggttatctaa gccggtccat tgtttgtgga
     9961 gcttgctggt aaacattttt acatccacaa acaatgtatc cacataggga gctacctttt
    10021 ggatttcatt ataagcacca caaaagctgg tttccagtac ggtattgatt ccaatgtatt
    10081 tcgatttttg caaaatttct ttagcaaaat ccgcctgaac cagtgcctcg cctccgctaa
    10141 tggtcactcc gccatccgag tgaaagtaaa aaacagcatc cttctcaatc tcatccatta
    10201 cctcttcagc ggtcatggtc tggccaaatc ctttttcaat ctgcttgctt tgtgattcgg
    10261 gaatcgaaca ccaggcacag cgtaatgggc agccctttaa ataaacaacg gtccgtaaac
    10321 cagcaccatc atgtagagaa ccctgttcta ttcttaatac tgttccttcc atatagattt
    10381 ttgaattaca cactagttta aactaaaaca cacaaggaaa aacttaccat ttaatcataa
    10441 aattagctca tataaatata ctaggatgta aaatagactg gtcaaaatct ggtcaaaaaa
    10501 gaggactaat acaggtagtc ctcttttttg ttattttaat attctaaatc attttttatt
    10561 ggtttattcc cttattcgat gtagactcaa atcactatat cactagatat caaacggcgt
    10621 catacggaat caaaaacatc aaatttgacc tgttaaatgg tcattgaata acattcaaaa
    10681 tcaataaaac ggagtcagac acggagtcaa aaacgctgag agtaaaaaat atatacagcc
    10741 ttgtaggtaa agtaccacag ggctgtatat tttttatttc agattacctt aaatgattcc
    10801 atttactttt tatctcattt tttcctacta gttagtatca aatactctcc tattttctca
    10861 ataaactatt tttggataca tggtatctga aacagctaaa accttgtcat tacagcaaaa
    10921 aatcacgtt
//
LOCUS       DACEMS010000095        10791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEMS010000095 DACEMS010000000
VERSION     DACEMS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.098"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            103..1149
                     /locus_tag="IW735_002098"
     CDS             103..1149
                     /locus_tag="IW735_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1045087.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1498..2130
                     /locus_tag="IW735_002099"
     CDS             1498..2130
                     /locus_tag="IW735_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1045088.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2718..4157
                     /locus_tag="IW735_002100"
     CDS             2718..4157
                     /locus_tag="IW735_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1045089.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4452..4670
                     /locus_tag="IW735_002101"
                     /pseudo
     CDS             4452..4670
                     /locus_tag="IW735_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4919..5134
                     /locus_tag="IW735_002102"
     CDS             4919..5134
                     /locus_tag="IW735_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045090.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5351..5607)
                     /locus_tag="IW735_002103"
                     /pseudo
     CDS             complement(5351..5607)
                     /locus_tag="IW735_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5794..6705)
                     /locus_tag="IW735_002104"
     CDS             complement(5794..6705)
                     /locus_tag="IW735_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1045091.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6724..7728)
                     /locus_tag="IW735_002105"
     CDS             complement(6724..7728)
                     /locus_tag="IW735_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1045092.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7725..9848)
                     /locus_tag="IW735_002106"
     CDS             complement(7725..9848)
                     /locus_tag="IW735_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1045093.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9853..10773)
                     /locus_tag="IW735_002107"
     CDS             complement(9853..10773)
                     /locus_tag="IW735_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1045094.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3300 a         2039 c         2022 g         3430 t
ORIGIN      
        1 aatgccgagt cttcaacaac ttcgtcaagt gaaagtagta cgatttcaag taccgaatct
       61 tcaacaactt caagtagtac aagtgaaata acttcttcga aagtgacaaa accaaaccat
      121 atagaatctt caaaaaagga agcgccaaaa acggaaggac aaacaaaaac tttttcgaca
      181 ccacagcaaa tagaatcaac agaatctttc caatcacaac caatactatc tggtaatgaa
      241 gaaattcatt ttgacaaaaa tgaatctgta gaaaatttta ttatgaaaat tggtgagtct
      301 gcgagaaaaa ttggtcagaa atatgattta tatgcttctg ttatgattgc acaagcgatc
      361 ttagaatctg ccagtggtca aagccagtta gctcaggcac cgaattacaa tttatttggg
      421 ataaaaggaa cttataatgg aaactttgtc attatggtaa caaacgaaga tttgggtaat
      481 ggaacgcttt atacgaccca atcaaaattt agagtgtatg aaaactatga ggaaagcttt
      541 gaagattatg ccaaattatt aactaaggga attagtggaa ataaagattt ttatgcagga
      601 gcattaaaag ctaatagtaa aacatatcga gaagcaacta aattccttac aggacggtat
      661 gcaacagata cacaatacta tttgaagcta aatgaattaa ttaaaaccta cgatttaacc
      721 aactatgata aagaagttaa aggtccacaa atgagtaaga ctggatacca agtgcctatt
      781 cataattatt caataaccag tttgtttgga aatcgtggaa atgaatttca ccgaggactt
      841 gatatggcag ctacacaggg tgagccgatt catgcagtta aagctggtaa ggttgttaaa
      901 gcagagtttc actactcttg gggaaattac gtggtgatac aacacgaaga tggatcaacc
      961 gcgttatatg ctcaccagca acaataccta gtaaaaactg gagataatgt tttgcaagga
     1021 caaataattg gctatgtagg aagtactggg aatagtactg gcccacacct tcatttggaa
     1081 atttgcaaag atagtagtct ttctcagagc atgttagttg atccgaaaac cttattattc
     1141 ggagactaaa aaaggtaatc aatctgacaa agtaacaaga aaatggttgg attcttagac
     1201 ctttgatcag gatattgaga ctggaattga tatctcgatt acgatgggtt tgacaaataa
     1261 aacaagtcca ttatcgaata tcgagtgtat tattgccgtc tttatgctca tattttgagc
     1321 aggtttcact agatgaaaac aatttatcta tcttgaggat ttcgtgtacg tatcgttcag
     1381 tcttgtattg taaattagtc ataaaatcag actaagaaaa attggaaata tttttttctg
     1441 atttattaaa agttaattga aattatatgg tacttctttc ttgggagggt aggtgcagtg
     1501 aaggaaagat atggtcacct tactgttgtt gaggagatta ctcataatgg atataaaaaa
     1561 tatcgttgcc tatgtgactg tgggaatgaa acgattgtat atcgatcaaa cttactatca
     1621 ggtagaacca aaagttgtgg atgtggaaat tataaaaata ggcataaata taaaaatttg
     1681 acaaataaaa cttttggtaa gttagttgca atcaagccaa ctaaactgcg aagtgaagga
     1741 actgttgttt gggaatgtca atgtctgtgt gggagaaaaa agttagtatc ggcaaagcga
     1801 cttctaaaag gtgatgtaaa aagctgtgga tgtttgaaat caaaaaagca tcacattgaa
     1861 aataaaaaat tcggattact tactgcaaag tatcctttgg acaataactg ttcatggaaa
     1921 acaaaatggg tttgtcattg tgagtgtggc agtatgtgtg agataagtta tagtaactta
     1981 ttttttggac atactcaaag ttgtggatgt ttaaaaaggc aagaaacaag gacatgtgtt
     2041 gaagggacag taatagagtg tctaacgtca aaattaccga aaaataatac aagtggtgtt
     2101 aagggagttt atcgttctaa agagaaatag attgcgtata taactttaaa aaaaaagagg
     2161 tactatttag gatcgtatga ttctattaag agtgcagctc aagctagaaa agatgctgaa
     2221 aataaattgt ttcaaccttt gattgacaaa tatcgaggta atcattaatt attgtggata
     2281 ataattaaca gtgatatata aacatctata atatattatt catactattg atatttagta
     2341 taatcctatg gaattgaata tcatagcaat gtatttgaaa agcaaattgg aaaacaaata
     2401 ggtgcaacac cataaatatg aaaaaattgg gaaaaagtag aatggaaatt tgcttacgtc
     2461 tcacgagata gctcacacta ttaatctgaa gaacaatgaa gtcgactttt agaaattgct
     2521 agaagaaagt aaagcgaatt atcactagtt actatagagt aagttcaaat aaagcatctc
     2581 caacttacat ggttacatga ggatatcaaa tcgaaattac tcaagcttcc tatggctaga
     2641 gtcgcgagaa gttgttgggc agtagacata ggttatattg ttcttagcta cacgatcatt
     2701 agaaaggaaa tcctattatg tatagttatc aagaagatgc aaaagtccaa aaaaatcgct
     2761 ggtggatttt gattgctata tctatgttta ccttcatgtc cacattggat ggcagtattg
     2821 tcaatattgc actacctacc atctcagatg atatgggcat accaatgaat caagcggaat
     2881 gggtagtgtc cgtttatctg atgattgtct gttcttgttt gttgctgttt ggtaaaattg
     2941 gcgatagccg tgggaaaatc aaagtatttc ggattggtac gctgatcttt gtcttgggat
     3001 cgctgttgtg tgggttcaat cattcgctag gtattttatt gttcgcacgg gtggtacaag
     3061 ccattggtgc tagtatgact atggctacaa atgccggaat catcacggaa gtctttccca
     3121 tacatgaacg tggtcgggca ttgggagcaa taggtgcgtt tgtttcatta ggctcgattg
     3181 ctggaccagg tcttggcggc ttgattttgt cccaattttc ttgggcgtac attttttgga
     3241 tcaatgtacc ggtgggaatc gtaacgatgt tgattgctga aaaattctta ccaaaagata
     3301 tcaccatgag cggccataca atcgatggag ggggctttgc tgccattgcg gctacgatta
     3361 tcagcttttt tggagcaatc tttttggggc aagaaagtgg ctttttccat ccagtccccc
     3421 ttgtactgtt cgtaatttca gtgattgcct ttgtcagctt tttaaaagtt gaaaagaaag
     3481 cagcctctcc attgatttct ttcggtattt ttaagaacaa gatttttacc atgagcttga
     3541 ttacagcctt gttgattttt tcttccaact tctttgtcaa tgtagtgatt cctttttatc
     3601 tgcaaaatgc tcgcgggttg agtcctagct atgctgggct gttgatgatg gtctttcctt
     3661 tgatgatggt cgtcggggcg cctttaagcg gctacctgac cgaccgggtt ggcccggaga
     3721 ttttggtatt agtaggcttg ggcttattgt cggtaaccca ggtcatgtat atgtttttgt
     3781 cagtggactc tcctttgtgg ctatacattg tcgctactgc catcatgggt gccggcaatg
     3841 ccttgttcca gtcccccaat aatacgatgg ttatgagctc ggtcactatc gaaaacttgg
     3901 gactggcagg ctcgatgaat tcctttgccc gaaatttggg gatggtcagc gggatttcgc
     3961 tttctacgac ggtcttatat caagcgatgt ctgctaagat gggccagcaa gtaactaact
     4021 atatcgcaaa tcggccggat gtctttttat atggaatgaa gattaccttt ttaggcagct
     4081 ttgttttgtg ttttgttgcc tttttcctga ctatctggcg tatcaaaaaa caaggaggta
     4141 ttcagaaaaa tgtctgaaaa aaccactgtt gaagaatttt tggaggcctg cgtatgggtg
     4201 agtgggattt ccatggatga tggaagatga agtgaacttc taaaagccga aaaactcaag
     4261 tattatcttc aaaatactag tgctcaatga gatgcttgat ttgatcacaa gaagactgcg
     4321 aaagccgctg gcaaccgcaa gctaggtatc ccaccgatgg actacgtttt agaggctact
     4381 ggttatgaat acggagccaa cgctctcatc tgcataagaa tacatcaccc agattatgta
     4441 aagattttcg atacttctgt caaaaaatca ttaagataga taaatgattt ccgtctagtc
     4501 aaattggttc agaatgtaga cacacagatg gtaagaagtc actcacaatt cgagaatgga
     4561 cttgtcctat ttgccgtata catcataatc gaaacgtcaa cgatagtatc aatattttga
     4621 ccaagggcta tgaatacaag cattgcctta gaaaacatca agaaccttag gaacaacggg
     4681 gatagcttgg ttaataagag ataccgctgt ttaggcttgc aagtatactc ttttatcaag
     4741 aattccgtgg ctttagtcat gagaggctta atactatgaa aattttcttt agcatttttg
     4801 atttaggttc cttttctaat tataataaga tgaccactac agtaacctac tgaaatatat
     4861 aaacagccac tgtagtacca ttttcaaagt agtctatgag aggagaatct tcattcttat
     4921 gaacgctgga attgcggata tgaatttaat aaaaaaaacc cttaatgatt ttacttccaa
     4981 ttcaatatca aaaggtactg gtatcaattt aagcacaata aaaaagctga aaagtggtga
     5041 aagatcagtg gaaaagctga atcttttaga tgctataaaa ataacggaat ttgctatgaa
     5101 aaatggaaaa gctgagattg aaatatggag ataattatcg tctagtgaat tacttgccac
     5161 ttagctgaat ttaacgatcc ttggtgcttg aagggggaga ttcttgggaa tagagcatac
     5221 ttgcaagcct atgtctttac taacaacgat atctcttatt aaccaagcta tccccgtagt
     5281 tctaaggctt ttacttttat ctaagcaata atcgtattca aaggaaattt ttgaccctta
     5341 gaaagtctca ttttatcaga cgtgagctta ggcattgctg aattttgtta attcggtttt
     5401 aggttgtgct ttgagcataa tatgaaagta ttctttatca cgatttcatt catctaaggt
     5461 aatgtgatgt gattctgaaa ttctttcaaa ggcagttttt gcataagctg acattacatc
     5521 atcaatgaca tgtttacaat gtttcactac taaaataagg gggtaaccaa gaagaaaaac
     5581 tgaatgatta tttgtatcta attccatggt atatcaacca tttcctatat agactgatta
     5641 tgacatggat taaaataaaa aggcactatg tcttaattgt cggttttcga gttaccgaat
     5701 ggcattcatc acccacctat agaggatggg cgacttctgc ctgaaataag ttacactact
     5761 tataacttct aataaaatta gattgagtca ttattattta acatcaacga ttttccagtt
     5821 tccatttttt tgtaatacta atttgtactg aaagtagcta gtggtttttg atacattatc
     5881 aaggtatttt atctttgtac tcactagaaa attttttccg ttttgtagat aaactggatt
     5941 ctctaactcc gaaaacttta agtcaatatt gattggtcgc atcgcaccat tttcaacata
     6001 atactccaat tctttttcag aagctgatgg ataaagttta aaaaatgttt ctaaaaaatc
     6061 ggtgatttct tccgttgttt tggcatcaat ttctgaatcg ttttctactt gttttgcttc
     6121 atattttgcc ttgtctggct ttccaatcat tgttggcaaa ctagttacta aatgattcct
     6181 gttttcatct tcaaagattg tcactcgata tgcagaagaa attgattgcg tcttttttcc
     6241 ctctgtaata tgttgctcca ctaaaaatga aacgacaaat tcttccgagt gtttttcaac
     6301 atctagaatt tttacagatt gaacttcgga actcgtggga atatctgctc gaaccatatc
     6361 ttggattaaa gcgagaccgt tttctgtaag gtactgttcg agattactca ttctcttttc
     6421 aattgcttct ttgttatttt tccatgaaaa atattctttc gcaaaatcct ctgtaaaact
     6481 ttcaacgcca gaagtattca ctaccttctc ttttataacc actttttcat gagttgtatg
     6541 ttgatcaatg gcggtgaaat ttttataaac gccaaaagca agactacaag acaataaaag
     6601 ccaaagggca agcaccattt ttcgatgctt acccacggat acaactcgtt cttttttctt
     6661 ttttgggctc ttttcctttt gattccgttc aatttttatc ttcattcttt ttcctctttt
     6721 ctcttatttg tatcggccag ctccgataag atgctgccgc caatagtttt ccgttagatt
     6781 cgtccacccc actggatctc ctgcatgaaa catgcgattc tcccctgcgt aaatgcccac
     6841 atgggtcaca taagtaccgg catcataggt tgaatgaaag aaaactaaat cacctggttt
     6901 ggcatctttt agatcaatat gagtcatggc atcatattga tcctgagcca cccgaggaag
     6961 tttcaggtca actttggcat aacaccattg ggtcaagcca ctacaatcaa accccgtttc
     7021 tggacttgag ccaccaaaca cataggccgt tccttcatac ttaaatgcct catcaaaaat
     7081 ttcttggact attttgtcat caaactgagt tgtagttagg tattgcttca ctaataaaca
     7141 gtaaaacata ttgccataat tataccgcca cccaccattt tccttaatcg cgattgggtt
     7201 ggggtaatcg actttttgac cacctgattt ttcctttgaa aaagattcag ctaaagcaaa
     7261 actatgcttc ttcccgtttt tcccgacatg atctagatac ccgccaccat agttataggc
     7321 ctgaataata ctatcttgat cacaccctag ttctttggaa cgcttaacca aagacgcaaa
     7381 atagagacag ccttgtttga tggatgcctc ggtacttaat gaattaggtg gtaatccggc
     7441 tgactcagaa ctttgcataa cgtcttccat tgtgccacca gattccactt gaataatcgc
     7501 caaaagaatt ggaatatact ctgttatatc cgattctttg gcatactttt ccaccattgc
     7561 acggtgtttc aatacttctt ctgagacact agttccgcca ggagaagtgc cagaactacc
     7621 atttccctca ctgtcttcac caaacaacaa acccacacac atcaaaatac ccataaaaga
     7681 gatggtcaga aaagttaatg aaataacacc cactttcttc aagttcattt tttctcacct
     7741 gaccttgatt ttggacgatg gttcattctt ttttgtactt tttgattggt ctgctttggc
     7801 agagtggacc tctgtttcac ttgttggaaa gaatttttct tacttgctga ttggcttttc
     7861 ccttttgaaa tgatttcttg actagttagt tttcgtttga tttcgggatt cagatttgct
     7921 ctcacaatcc gctttggtgc ttcttttttc agcatcatat ctttctgagg ttgagctgct
     7981 ttatctattg gaatattcgc ccttcttttt ccctgacgat ttgttgcttc actcagtatc
     8041 ttgcgttttt cgtccatttt cgcatttctt cgttgtcgtt gtttttctcg caaatccact
     8101 cgatccgctt tttcttggaa cagtccgcgc ttgaattctt ctggtgcttt tttcgacttt
     8161 tcgattcctt tatggagatt gtatttcaaa ttcgttggcg tattccttac ctgatccttt
     8221 acttgtttag tcttatcttt caccaaagct tgggtatcca aaactttgcc gatttttctc
     8281 ccagccaaat tcattcggga ttgtttctta ttatttgaaa ctggattctc cttagttaag
     8341 tcagatagta tttcgtgatc gttcggtaat cgtgaattct ttcgtagaga agaaccggat
     8401 ggcttaaatt tggattttga ttgttccttt gccaacttat ttccgactgc tgcaccacca
     8461 ccaaaagcca aagctcgtcc cacatttcga ttgagttgcc gcaattttcg attcattaac
     8521 atttgcggtt ttcgcatgac gcgtcttccc attgactggc tatcattact ctggaggttg
     8581 aacatactca taatgtcgcc caatttgaaa tagataccgg caaaggtcac aatttgcaga
     8641 aaagcaacca tgaaaaatgg ataggttgcc gaaatattga aaaacatcgt ggagatgcta
     8701 aatgcagtcg taatcaccaa agtaacaccc gctcgcatca taatcgtatt gaataaacga
     8761 ataatcgcct tctttcctaa actttcataa gtcggcaaca tggacaataa gaaactaatt
     8821 ggcagaaaca tcgcaaagat aatgaagaga atttgcgaga acaacataat ccctgttaga
     8881 agaaaaacaa agaaggaaat gccaatattg aacaatacca aaaagaccac ggtccctaaa
     8941 cggttcatgg tcttggtaat actgagattc gcattgtcat tgtcttcaat ctctgccttg
     9001 accgcttctt cccgatcttt gcctttgttt tcatctggac tcacagatag aatcttgttg
     9061 acccggtctt caccaatttc ttctatgttg gaatcatcaa attgcagtag taaccaaggt
     9121 tgttggactt gaatcgaaaa caaactatcc cgaatcaaat ccacactatc ttttccttgg
     9181 ctttctgaat tgggtacaac gatctcggtt ccaagggtaa gggccgcttc actaacatct
     9241 gagctaaagt cattgatttt tgtgatatag gtaggcgcat aagcaatgaa cgaacccgat
     9301 agtagaaaaa tcatcacaaa attaatcacg gctcgaactg ctttcgtggt ttctcgtttt
     9361 aataatccgg tatacgccac atacaccccc agagctaaaa tcatcaataa tagaaaccca
     9421 aaataaaatc cggaggtctg gagtccattt tcagtaatac cagccaatgt ttggatattc
     9481 tttccaatac tttcagccgt atccgaaatg aaatctaatt tgtaagcctc ttgaaccaca
     9541 taacccgtgg cgttcgacaa atacaaactc accgtccaaa tgaaattagt aatggcataa
     9601 agaccataca tcacgctttt ccctaagcca tcaccccaat tccaaggcaa ccaatcccaa
     9661 gagctatcca caaagaaatc tagttgatag ttgttcagtg aatatttgga atatagattt
     9721 cctgcttcaa ctgtatcatc aactagccct gtcgcctcgg ctaccgtacc aactaaactc
     9781 aatagaacta tcagagtgag tatgctaata aaaacgattc ccacaatccg aaaaagtttc
     9841 tttttcatcc cctcactcca ttctctcttg aacaggtgga cgggtatcaa aggcatggaa
     9901 gagttcttca aagactggat gtacctgaac cacgcctacc cgaccataca aatcctgcat
     9961 caagcattgg ccattttcca aatctcgtag gcgtttttga ttgctttcat cttctcgatc
    10021 caagccaaag aattccaagg ttttcttgat ttcatgaata tcggtactgc gaaacgcaaa
    10081 cttcaacccg atattattct tcaagcgttc atccagtaaa tcatcactat tttgcgtgac
    10141 aaagtaaacc ccggcattca tagcccgccc agcacgaacg agcttattcg ataatgtttt
    10201 cccttgcgct acttgaagaa aactccacgc ctcatctaag tctacaattt tgaagatttc
    10261 tcgattacta tggataaagt ccaaggcaaa agtggaaatg acgattagca tggaaacact
    10321 aagcaattcc atggtggtat attcttcaaa agtggtttct gcatctggta aaaccaaatc
    10381 cgctacttga atgatattta actgcctatc taaactaatc gactgggtaa tcgaaccatc
    10441 tgagaaaagc aaatgggcaa aatcataatc ggtaaaggac tcgatgtgat ccgcgatatt
    10501 ttccgcaact ggtgagcctt cttttcgaag ctcattaatt acttgtaata atccccgttg
    10561 attgctttgg gtaaccgtcc gaatggcctt acgcaacact ggaaacttct ccccatctcg
    10621 actagaaatt cctgttaaaa aggtcagaat atcaattgct agactttcag aatccttctt
    10681 tttctccata atcacataag gatcaagtaa tccttgattt tttggcttac tagttaagtt
    10741 gagaatattg atttcatccg caatttctgg caaggtttct ttccagccac c
//
LOCUS       DACEMS010000096        10660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEMS010000096 DACEMS010000000
VERSION     DACEMS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.099"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..369
                     /locus_tag="IW735_002108"
     CDS             37..369
                     /locus_tag="IW735_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045095.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            370..963
                     /locus_tag="IW735_002109"
     CDS             370..963
                     /locus_tag="IW735_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045096.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            985..3009
                     /locus_tag="IW735_002110"
     CDS             985..3009
                     /locus_tag="IW735_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1045097.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            3009..4208
                     /locus_tag="IW735_002111"
     CDS             3009..4208
                     /locus_tag="IW735_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1045098.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            4224..4868
                     /locus_tag="IW735_002112"
     CDS             4224..4868
                     /locus_tag="IW735_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045099.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            4879..5685
                     /locus_tag="IW735_002113"
     CDS             4879..5685
                     /locus_tag="IW735_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045100.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            5708..5937
                     /locus_tag="IW735_002114"
                     /pseudo
     CDS             5708..5937
                     /locus_tag="IW735_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6096..7031
                     /locus_tag="IW735_002115"
     CDS             6096..7031
                     /locus_tag="IW735_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1045101.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            7075..7344
                     /locus_tag="IW735_002116"
     CDS             7075..7344
                     /locus_tag="IW735_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045102.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            7620..7895
                     /locus_tag="IW735_002117"
     CDS             7620..7895
                     /locus_tag="IW735_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045103.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            7937..8359
                     /locus_tag="IW735_002118"
     CDS             7937..8359
                     /locus_tag="IW735_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045104.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            8379..9971
                     /locus_tag="IW735_002119"
     CDS             8379..9971
                     /locus_tag="IW735_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045105.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            9992..10327
                     /locus_tag="IW735_002120"
     CDS             9992..10327
                     /locus_tag="IW735_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045106.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            10473..>10660
                     /locus_tag="IW735_002121"
     CDS             10473..>10660
                     /locus_tag="IW735_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045107.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         3924 a         1629 c         2233 g         2874 t
ORIGIN      
        1 tctaaaaata caaaatataa ggggtatggt tatattatgg ataaatacat tcatatgaaa
       61 aatcctccaa gatttagtgg ggaatggaag tttttcgcct ttttcttttt agatctatta
      121 acgcttggtg gctgcttttt ttgggcgaaa atagtcaata atcttgtcca cttaccactc
      181 ttttttgcac tcttgaatta tggattatgg cttgcgatag cgttagtgat tgtttggcga
      241 ccctataata accctggtca acgccaaatt tttgcgatta tcgaatctat tttctttacg
      301 gataagaacc actatcatgc gttagatcct aaccgaaatt atcatggtac gtataaggag
      361 gatgaataaa tgcgagtcaa aggacaaaat tcagttgata agaaatccat tttgcagttt
      421 ggaaaaccag aagagcgctc tatcttggac ttatttccat ataaatcctg tacagaagag
      481 ggagtcttaa ttactagaga agatcatttt cagcgttatt atcgtgtcgt ttccactgac
      541 gtggagggat taaatgaaca agagaaacta gaacgaatga atcagttgac caccgtcatg
      601 cgtacgtatg tgccaacaat taagttagtt agtttgacca cagagacgga tctaacggaa
      661 caaatcgtgc aaaagcggca gttattagat aaaaatcgta tggaacagtc actaggaaga
      721 aatctacagc gattaagaaa atatgaaaag aaattagtag aggagatcca ggaattaaaa
      781 cgggcagaac aagaacgtcc tgacttaagc ttctttttcg ttattgaagg aaagacaatc
      841 aaggatttac aaactagaaa tcgtcagtta atgcgatcta gtggcatttt agggttaaaa
      901 ccactctaca aaaaagaatt gattaagatt ctttatcgaa tgaacaacat gaatgacgaa
      961 taaaaaagat ggaggaaatg attcatgcta gatgtgataa aaacagcttt ttcaacgcaa
     1021 agtaatcaaa aggtgattga tccattaaaa caacttggta atcgtgaaat agagatttta
     1081 aaatcaaaag gttatgattt agatttctta aaacaagtga tgccacaagg tgggttgaat
     1141 tttgatgaag aatatgtgac ctatggaaat ggttattcac gatgtgtgac agtttatcat
     1201 tacacaaaaa atcctactct tttttggttg gccaatctca tgaacaatcc tcatacagtg
     1261 gccacactag atgttagcac agatgaaaag caaaaagtga ttgatcaaat caaccgcgca
     1321 ttagatgaat taaatgatcg atcagataat gaacgaaaag cgactgaccg aaataaaagt
     1381 gcggaagaat acctggaact agcggaatat gcccgaaaat tgacttctaa tggggaaatt
     1441 tctaaacaag tcaaagtgag agtacatgtt tacggaaata cgatccaaga agtggatgaa
     1501 cgggcgcagg aactgatcga aaatctaaaa gggatggatt atcgtgcggt gacttatctg
     1561 cataaaacca aacgagattg gcaatcgatg tttaccatgt tcgatgaaca agctgaatgg
     1621 tttggaaata tcaaagggca atcacttcca tcgttaaaca ttggcggagg attcccgttc
     1681 caccaccaaa cgctaaaaga tccaggaggc acacaatatg gggaaaccat gacaggcggc
     1741 ccatttgtct ggaatgccac gtataaaaat agtgtacgtt tatctaccaa taccttgctt
     1801 ctaggaatga tgggagctgg taaatcgacc gttcttaaaa tgatcgcgga agcccattta
     1861 gctgcaggag actttatttg gggctttgaa aagggaaaag actttattcc tttcttaaag
     1921 gaatataacg gtattatggt gcgtttagac ggatctgacg ggatgatcaa tcctttagag
     1981 atctttgcca cacggacgta tgatgaagca tccagtttat atgacgatgg ttcagtcaaa
     2041 gatttgaaga tcaatgaagc agcgtcctat caaacgcact tagacaaagt agtctatcag
     2101 gtacagttag tgagtcctca gctaaaagga acgatgaaag cagaattcaa gacctacttg
     2161 aatcgttttt atgaagagta tggaacagta ccacgcggtt ttacttcctc aaattcgcgt
     2221 tcaaatactg atacgcaagt cacagggaaa gatccagaag cttatccgac gttcaaagaa
     2281 tttttagcct ttttaggaca actggaacta ccaggggctt cccaagagaa aaagaatcgg
     2341 aaagaagaaa tggagtctat cgtggaatcc ttatgtgaga cttatggcat gatttttgat
     2401 ggtcatagta cgattcgaca ccttgaccaa caacagttgg tcttttttga cattgactct
     2461 attacaggcc tagatcgagg ggtacaacaa tgtcagatgt acatggccat gacgttgatt
     2521 tggaatcagg cattgattca aggacgaaga caacgacgct taattaaaga aaaacaaatg
     2581 gaagaacaag acttcaaacg cttcttggcg atgattgatg agtgccataa tgcgattaat
     2641 ccagatttca ttgaaacggt aaattatgtc acaaacttcc aacgagaaat gcgtaaagta
     2701 gaagcaatga ctgttttagc tacacaatcc ccacaagaaa tggtgccaga aaatatgtct
     2761 tctgataact tctcgaaact gaagaaagtc tttgaatttt cttctacgaa gatctttatg
     2821 cgtatggatg aatctatttt acgtcatata gaaggattag ttggtaaatc aatgacgaac
     2881 tcagaattag aatccattcc tcaacaaaat caaggagacg ctgtgatcaa ttttggctca
     2941 aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc aattaaaatt gtttgatgga
     3001 ggaaaataat ggaaaaccaa aatgaaagtt taatcaaaca gtatgttaag cgtcgtgcaa
     3061 agcggcgctt atttttgtgg gtgtttggta cttctgcagg tttgattact atattgatca
     3121 ctgtgtttgt gacccttttt ctaatacttg cggcaggttc tattgataat tctgattcaa
     3181 gtagtggtgg cgaagctttt acaggagaat attcggaagg cttacccatt tataaagaaa
     3241 tcaaaggaag aggccctttt tctgatgaga ttgcgcaata tgctgtaggt gcagcagtga
     3301 agtataaatt attaccaagt gtgatccttt cgcaatacgg ttatgaatca gcgtttggta
     3361 cgtcggcgtc cgcaagaaat gatctcaatt attttgggat tacgtggttt gatgggtgtc
     3421 tgtttcctaa aggaactgcg cgtggaattg gtggcattga aggtggctgg tacatgaagt
     3481 ttcccaactc aaaagctgcc ttctcctatt acggctttat ggtagccaca caatcaaatt
     3541 ttaatgcttg tgtcggaaat aaaagccctg gcgcaagctt attgatctta ggacgaggag
     3601 ggtatgccgc cgcaggaatt actgaagaca gtgcctatta taaaaattgt atgtctatta
     3661 ttaatacaaa taaattgatg gagtacgatg agtttgcgat taaacactgg agagaaggcg
     3721 gcggaactgg tggtacgatt acaggaagct ggggcaatcc attcccagga agtagcttag
     3781 ataaaaattc gttcagtgga ggacaacttt ttgggaaaaa tccaggagga gaattccgac
     3841 ctaacgggtt ccatgatgga ctagactttg gttcagtcga tcatccagga agtgagattc
     3901 atgcggttca tggcggaaaa gtcgtgtacg ttggcaatcc tggtatttca ggtttgggtg
     3961 cgtgtgtgat cgtgattaat tacgatggct tgaatatggt gtatcaagaa tttgcaaata
     4021 gtacaggaaa ttctcgagtg aaagtaggcg atcaagtgaa agttggtcaa gtaattggga
     4081 tacgagatac agcacacttg catttaggat ttactcgaat ggattggcgt caagcacaag
     4141 gacatgcatt cattgatgat ggtacatgga tcgatccgtt accatttttg aatagcagca
     4201 aaaaatagat caggagaaaa aatatggaaa ataaaacctt aaaaatcgtc ttaacagctg
     4261 caattagcgt tagtttaatc ggcaatattg cgttgttttg gcagcttaaa gaagaacaag
     4321 agacaaatac ctataaaatt gaagaagtgg ctaaaaacgc gtctgataag ctcaaaaaaa
     4381 attcttctga agcacaggcg aagattgaca agttaaatca aaccatcaat gagaaaaacc
     4441 aagaaatcga tcagctgaag caacaagatg gcacaaaagc aaaagaggaa gtaactgaag
     4501 cacaaagaaa gacggcagaa gagttcggtc aattggccat tgataaatca ttaagcagaa
     4561 atgaattaac ggaaaaacta aaagatgtcg caacccaaga agtgatcgac aaattaagtc
     4621 ctgtagacga tgatcaccaa catgatgatt acggaagtta ctcttttaca ttaggtgacg
     4681 tgcaaactta cgctcaaaca agcagtgtaa aagaggaaca aaattttgtc gtatttgttg
     4741 attatacaat cggcaatcct caattcaaag atgtcaaacc acaaaaaata aaaggtggat
     4801 taactgtcac tgaaaaacaa gtagatggtc aatggaaagt cacagacttt agttatttca
     4861 ctcgttagga ggaagaaaat gagtaagaaa aaaggagcaa gacaacgttt taaaaaacga
     4921 aaaagttatc ttagatggct ggggatcttt atgattctcg gctttttgat tcttgctagt
     4981 ccgatttaca cacataaaaa ggtagacata ccagatgaag taagcacgaa cgcattttta
     5041 aacacgaagg aactgacgat ggtttcaaaa gaaaaaggag cgaaccagca attagttttt
     5101 gaatttgcga tcgacgatga agatcagagc ttattaaaag aactagctaa tcttaattat
     5161 cgtgtagaag taaagacagc taaaggggat tatcaagcaa taaaagccaa agtgatcaag
     5221 gtgtctgatg attattttgt agtgaagtta gcacatgtac cagaagaata tattgctatg
     5281 cgattaacta ttattcctga aaaaattgat ccaaaggtgg atatgcaaga accgcaagat
     5341 ctgatttttt atattcacga agataaggtc aaagatcacg tgaaagacga agattatgaa
     5401 caacatgcga tcaattacaa agtgaaagga tacaagaaag aacaaaaagc agcacaaaaa
     5461 caaattgaat ccttccaagc aaccattgat ttgaatgaag aattagtgaa gaaactaaaa
     5521 gatcaattgc cttatcaagt cgcagaagat caggaaaata ctgaaaataa gattaatagt
     5581 taccagcaag aaattgaaac atcgaaaacg caaatgaaag atcaagaaga gagcattcaa
     5641 aaacttcaag aaaaaattga tcgtatgaca aaagaaaata tttagaagaa taggtggagt
     5701 aaaagaaatg aagcgagcga acttggtcgg caaagatact ttcgctgagc agaaagtatt
     5761 gattaaagcc tatctcaaag ttgtaaaagc taaagaaata tgtcaagaag tgaattatct
     5821 aacacaacaa tatctgagtt gaagaatggt agaaagagtt tagatacctt aaaattcaga
     5881 tcatttgaag ctctatatat agctgctaaa aaatatttta cagctgataa taaataaaaa
     5941 attaaggtgg gttctatgtg aaaaaaacgt atcaaaagtt tttatggatt agctatatcg
     6001 tcctatttat cgttgggttt ctcgtcagtg tgtccgtgat cgtcggaacg ctttttcttt
     6061 tgttctctga cgaagatctg gtacttaagg ggttaatgag tacgttatcg tgtgtcatgt
     6121 tactttattt atgtatacag atcaacgaaa ttatgccccc tcattggcaa gaaaagaaga
     6181 aagaatttaa ggaagaaatc gaaataaaat ccaatgactg gattgaatac acatggcttc
     6241 ctctgaaaag aaatagcaaa cgaaaggaat tttgggcatt gcaattatgg ccaataggaa
     6301 aactttttat ttatatggct tcacttatgg ttactttaat cggttatttg gtggtggtct
     6361 atggggtggc ctattacttg ttaagtgagc caaaacagat cgcggcgtca aatattttca
     6421 cggcagtaat gacattaatg tgtgtcatgt gcttttctat ttttagttac ttactcgtcc
     6481 aatttatcag gcaaaattta acaatgagta aacttttcaa aagtttattg gacttactta
     6541 tcttaggcag tatgttaacg actctaacag atcggttcga acaatctgaa aaaacggtag
     6601 aggggataaa gaacttctta acgacgattg atcaatttat aaatggatat atgtatttgt
     6661 ttgaagtagg ctttattctc actttgtttg gttgtgtatt tataaaactc attaaaagat
     6721 caatcgaaaa agaaatggtt attgatctaa ttccgacgct gagcggctat gagtattacg
     6781 ttgattttgg aaagtatgag gagttgatcc atacggatat cgttcacacg agtatccatg
     6841 aagtaaccgt taagggcatc agatctactt taaaagatgg acaggagacg aaaacaatcg
     6901 accgaagaaa agaggtctac aatttttata aagttgaagc aagtctacga tatggcattg
     6961 ggaaattgag gttagatggg agtaatattc aacaaaacaa accatatgtt ttaggaaaag
     7021 tggtgattta gaacttccta tgaaagatga ataaataagg agaatctttt gaaaatgatt
     7081 cttctttaca gaattgtgct tatagttggc acggagttaa aaatcttaaa gaaggagcag
     7141 ttattcaaag tgaaagaaca tcataatcag ttactatgta ttaaaacggg tacgatgaac
     7201 attcaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gtcgattcga
     7261 gagcaacata agcaagaaaa attaatggct acttatagcc aattgattga tgagtgggaa
     7321 caattcaagg aatctaaaac ataaaattcc atttaaacac cttatttttt aaagagcata
     7381 aggaaacgtg tacccacttt caaaataaag tgggtagata ggctgtgcct atcacgtttc
     7441 tgaaaaaaat aaatgacccc aaaattttca atttatgtgt aggtttatgg gtagcaaaaa
     7501 tatgaatgtg ggcagcaaaa agttcatttg tgggtagtaa aattcaaatg tgtgtagcaa
     7561 tgtgtgtagc tacacacatt gctacacacg ctataacata tagtgaaaag gagatattta
     7621 tggaagtaaa aataagagat attgatgaaa aaacgattca aagaattgac cgaagatgta
     7681 ttgaattaag tgaacgcacc gggactaaat ggagccgaaa tcgataccta aaagttttga
     7741 ttgaaaatga ttttgaccat gaacttatgg atcatagaaa agatcaattt gatcggttgt
     7801 tagaaaaatt tgtggatatt caaacttata atacgaaaat tttagaagaa tatattgcaa
     7861 caaataatca attaatcgcc ttgttaattg aataataaaa atagtattgt gaatttataa
     7921 aaggaggaaa tcaattttgg caaaagcaac aagaaaacat gtatatcttt acgatatgca
     7981 tatcgaagta cttgatgaaa taatttctac tcatgaagat atatctaatt atagtgaagc
     8041 agtcagacat ttgtgtatga tgtatgatcc aaaaatcgaa gaaaaaataa atccaattca
     8101 aattaaattg aatgcaatgg gaaaagaaat ttctatatta accgaaatgg tttcagagtt
     8161 tgcggatctt catttgaaag atacaggaat actaattggt acaaattcaa ctgtctatca
     8221 agaagcaaaa gagcgtgtgg aagggaaaat aaaagcgaat caaacgaaaa agtattctcc
     8281 taagatcaag aaaccaataa atgaatctgt tcatgccaga gtagacccgt ttgatccaac
     8341 aaagttttta aaaagataaa aagtagaagg ggaacatgat gaataaatcg cctgcagtca
     8401 ttctcacttg tcaatttacc ttgcccaata aaaaatcttt ttcaacatac attgattata
     8461 tgacaagaga aaaagcgctg gaagaaaagg aacgacgcac gccggacgaa gaacaggaac
     8521 tgcaacgaat caagaatgct ttagaggatt tttatattcc tgaaggagaa actttttcgg
     8581 agagtaagac agataaagaa ttcagtgaaa cgtcgaatga agctcaatcg ttgatggaac
     8641 atggaatcaa ttttgacaag ttagaggaac aagattttac caaatatctt tcttatatga
     8701 cccgtcacta tgcgttagaa caaaagaaag aactgacgca aagggaacaa aaagaatacc
     8761 aacggttgaa ccaagcaatg aaaaaatatc aaacagagcc acataaaaaa gcttcaggaa
     8821 atcttcctgg agttttttct atggcttcag acgaagtgaa aggaagtgat ctgaaggaga
     8881 tcaaacaaat cttccaaaga gggcaacaaa aaggctcaat catttatcaa gatgttgtct
     8941 ctcacgacaa ttcgtatctt gaaaaaatgg gcttgtacga tcccaaaaca gataccttag
     9001 atgaagaagg attaaaagca gctggaagaa aaatgatgga gacattattc gagaaagaac
     9061 aattgaatga aacaggttac tggatggcaa cgattcaccg taacactaag catatccata
     9121 ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc ttacacggag gttgaaaatg
     9181 gtgtgagtta tatcgtaccg aaaggtaaga gaagacaagc aacattagac gcaatgaaga
     9241 ccaattatgt acatgagtta gaacgttttt cttatgaaaa ggatcgaatg attggtatgg
     9301 agaaacgaaa tttattgacg cggaaaagtg atttgcgtaa caccttaaca aaagtggtaa
     9361 aagagccagc gcgttacgat cgacacgcaa tgaacttatt aaatgaagta tataaaaccc
     9421 tacctgacaa acgttcggaa tggcattatg gggatgaaaa acgaaccaaa ctttcaccat
     9481 acacaagaga gaaactcgat gagttaacca aatatgtgtt aacaaaagaa cctgaatatc
     9541 aagaatatat acagatttca aaatcattga aagaagaagc aaaaagtctt tatggggaaa
     9601 gcaagagaga atcaaaggat gcagaaaaaa atgctctctt tgatttgaag aagcgcacag
     9661 gaaatgccat tctaacagaa ctgaagaaac aggatagtca aataaaacct ttaatagaaa
     9721 gtatggatca gatgcaatct atttacaatg aacgatcctt tcgaacgctt aaacagttac
     9781 caacgaatga atacaaatat agttacgaag aattcatgaa gcgcaggaaa caattccaac
     9841 aaagagttgt cacgcaacgg agtttgcgtc aattaaatcg aacgattaat gcccatgaag
     9901 aaaaatacca cgcaagtaaa gcctacgaag agcaacaacg aagaattcaa atggaacaag
     9961 aaagacagta aagacagtag gaggaatagc aatgaaacaa tatttagatc aatggaaagt
    10021 tatagaagga tcattaagag aagaacgaat cgaacaactt cccgattgtt tagaaaaaga
    10081 gcatttgttt cagataagag aaatgttgag aaacgaacag tttgatccaa atcaattttt
    10141 agtcgttgag tactcagcta ctggtgtgta ctgttgtaat catgtgaagg gtgaaaaata
    10201 ttttatcatt caagaatatg agggcaagtt agcgccttat tatactactt gggaaatgaa
    10261 tgaagagggg atcaataact tcccgtgtaa aagcattgaa gagtctattt cactcacaga
    10321 atgttagttg ttaagtgaat aaaagaagta aaagagtcct gaacgtaatt gtccaggact
    10381 ctttttttca atgaattgtt ttccgccaat tcaatgagtg gtgcgatttg cgactacatt
    10441 atatcaaaag aaagagaagg gaggaagaag aaatggctta ttcaacgatt ggagttgttg
    10501 ttgaaaaaag cagagacaat ctcgtttttg tgacagaaat tcaaacgggc agagcttttg
    10561 tcgtcacaga caaagcggca aaagcgtatc aaagtggcga tattttaacg ttaaatctga
    10621 ctacgaagac gttcgtcgat gcggcagaag attatccatt
//
LOCUS       DACEMS010000097        10437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEMS010000097 DACEMS010000000
VERSION     DACEMS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.100"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..309
                     /locus_tag="IW735_002122"
     CDS             91..309
                     /locus_tag="IW735_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045108.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(598..1110)
                     /locus_tag="IW735_002123"
     CDS             complement(598..1110)
                     /locus_tag="IW735_002123"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1045109.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1124..3421)
                     /gene="recJ"
                     /locus_tag="IW735_002124"
     CDS             complement(1124..3421)
                     /gene="recJ"
                     /locus_tag="IW735_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1045110.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3563..3997)
                     /locus_tag="IW735_002125"
     CDS             complement(3563..3997)
                     /locus_tag="IW735_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1045111.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4007..4795)
                     /locus_tag="IW735_002126"
     CDS             complement(4007..4795)
                     /locus_tag="IW735_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1045112.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4792..5733)
                     /locus_tag="IW735_002127"
     CDS             complement(4792..5733)
                     /locus_tag="IW735_002127"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1045113.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5910..6497)
                     /locus_tag="IW735_002128"
     CDS             complement(5910..6497)
                     /locus_tag="IW735_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1045114.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6718..8031)
                     /gene="obgE"
                     /locus_tag="IW735_002129"
     CDS             complement(6718..8031)
                     /gene="obgE"
                     /locus_tag="IW735_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1045115.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8319..9329
                     /gene="gap"
                     /locus_tag="IW735_002130"
     CDS             8319..9329
                     /gene="gap"
                     /locus_tag="IW735_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1045116.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9414..10349)
                     /locus_tag="IW735_002131"
     CDS             complement(9414..10349)
                     /locus_tag="IW735_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1045117.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2888 a         2356 c         1809 g         3384 t
ORIGIN      
        1 ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata tacgaacaat
       61 tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct tggcttagta
      121 ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc agctttactt
      181 gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa
      241 tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta cagatccagg
      301 caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa cttttacggg
      361 ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta aagccagttt
      421 ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg gtctttgctt
      481 gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct gacttttgag
      541 ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt tttctgctta
      601 atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat ggagatccat
      661 caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga tcagttcgat
      721 cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca cacgttgacc
      781 tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta atccataagt
      841 cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa aaccgactcc
      901 taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc caactaccat
      961 atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc gataagcgtc
     1021 accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg gatagtcggg
     1081 aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt gttaaccatt
     1141 tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt ttattgagcc
     1201 gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg actttttgta
     1261 aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc ataaaaatta
     1321 ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga cgtatatcaa
     1381 ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt gtgcctaccc
     1441 cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg aatgaataag
     1501 accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca actagagagt
     1561 ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa acaatcgcag
     1621 cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct aaataaaggc
     1681 taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca aatacttgca
     1741 gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg ttccattcat
     1801 tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct tcttgagtac
     1861 caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca aatttcagat
     1921 gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga aataaaaact
     1981 gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca attcgctcta
     2041 ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct ctcatcaagt
     2101 caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg acattttcac
     2161 ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca
     2221 tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat cctttagctg
     2281 atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc attattttcc
     2341 cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga tggatatgat
     2401 tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt gcttttcttt
     2461 cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg
     2521 caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt agtctgggag
     2581 caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa
     2641 atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct aatgaaacaa
     2701 ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc gctactaaat
     2761 ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat gctactccga
     2821 ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga acaatcgcat
     2881 aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg tctacaccag
     2941 cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca gtgatgatca
     3001 actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca tacccatgct
     3061 caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt gtttctttca
     3121 ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg attttttctc
     3181 cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt atcagatacg
     3241 gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt tcctcatggt
     3301 aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca cggatcattt
     3361 ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt gcttgtctca
     3421 ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga
     3481 aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga tcttttgtac
     3541 gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg ggcgattgag
     3601 ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct tgtaactccg
     3661 cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt
     3721 ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc tgagtcagct
     3781 gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg
     3841 caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag gaagtgaagc
     3901 caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata atcaaaacta
     3961 aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac tttttgttaa
     4021 aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa
     4081 cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg cggacgatca
     4141 tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa tagcttccgc
     4201 ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact gtcgtaacag
     4261 ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg actgcaaatt
     4321 tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata gaggcgatat
     4381 taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca
     4441 tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga
     4501 agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc ccgacttctt
     4561 ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct accttgaaag
     4621 cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct acaaatgcca
     4681 ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa cagatttgtg
     4741 cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa ttcatgcctc
     4801 ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg tcgtgttttc
     4861 aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat atctcgcact
     4921 gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag cagcttgatg
     4981 agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa atgtgctttc
     5041 atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc
     5101 gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt ttccattgat
     5161 cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt acagtggtcc
     5221 agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat gatcatgttc
     5281 aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat aaacagaaaa
     5341 ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca gcggataact
     5401 taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc cttttggccc
     5461 gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga gcccgggaag
     5521 tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg
     5581 aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca accaaacttc
     5641 attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt ttgcaggaac
     5701 acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct ttaagttcct
     5761 ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt
     5821 aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa acagcagcga
     5881 acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact ttcattgtcg
     5941 ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt cgcatgattt
     6001 tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa gcgagtaatt
     6061 tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc ttcatcagca
     6121 atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc gaacacggtc
     6181 tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat
     6241 cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat taatccaccc
     6301 gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc
     6361 ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg aacagcatgt
     6421 aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa atttaaaatg
     6481 attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt attttacctt
     6541 taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg gctgtgacaa
     6601 aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga tcacttctgt
     6661 cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt tgcttgccta
     6721 ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt tcgcaccgcg
     6781 tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac gcatcactgt
     6841 ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac cagataagat
     6901 ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat tgaatccata
     6961 gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg ttacttctaa
     7021 caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc cagaaattgg
     7081 aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct gttctttgaa
     7141 tttcactaga ttttccggtg catccggcat atccattttg tttgccacga tgatttgcgg
     7201 acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa gatagtcttc
     7261 ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta ctcgtgttct
     7321 ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc cttcaatcaa
     7381 gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca ttcctagatt
     7441 aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg aaataacgga
     7501 tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg ccaatacttt
     7561 caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc
     7621 aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac cttttgccac
     7681 aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct ccgcatctcg
     7741 gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac cgtgcatccc
     7801 tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat
     7861 caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac caccgtctcc
     7921 gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc
     7981 gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca taattaagtc
     8041 ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca aaagtcttac
     8101 ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt gtttaccttc
     8161 ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag tataattttt
     8221 tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa ttgaggatgc
     8281 cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa gtaggtatta
     8341 acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc tcagatgata
     8401 ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa ttattgcaat
     8461 ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac tcaatcgtag
     8521 tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc ccttgggcaa
     8581 aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct gaagaaaaag
     8641 caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca gctggtgcta
     8701 tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac aaaatcatct
     8761 cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta aataatgaat
     8821 ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca caaatgctct
     8881 tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct gacaatacta
     8941 tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag ctacaaggaa
     9001 aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact gaattggttt
     9061 cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa aaacacacag
     9121 ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat gtcattggaa
     9181 caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct ggtgatttcc
     9241 aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc caaatgattc
     9301 gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa agaagccttt
     9361 gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact cgttcaatga
     9421 acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc tgtcttgtat
     9481 gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt cgttgcacga
     9541 tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg tataaagagc
     9601 attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa aaagtagcca
     9661 gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg aagactaaac
     9721 atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca atcttcaatg
     9781 attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt tgctgctccc
     9841 atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag cactttgttt
     9901 atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc cccttcgctt
     9961 tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg aaacatcttt
    10021 gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag agctgttttt
    10081 tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc ttttgaagtg
    10141 atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc cgtttctttg
    10201 gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga aggcacaaca
    10261 gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc taaagcgacg
    10321 cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc ttttttcata
    10381 taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt ttataac
//
LOCUS       DACEMS010000098        10414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEMS010000098 DACEMS010000000
VERSION     DACEMS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.101"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="IW735_002132"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="IW735_002132"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1045118.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="IW735_002133"
     CDS             1007..1927
                     /locus_tag="IW735_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1045119.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="IW735_002134"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="IW735_002134"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1045120.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="IW735_002135"
     CDS             3233..4711
                     /locus_tag="IW735_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1045121.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4988..7048
                     /locus_tag="IW735_002136"
     CDS             4988..7048
                     /locus_tag="IW735_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045122.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7131..7700
                     /locus_tag="IW735_002137"
     CDS             7131..7700
                     /locus_tag="IW735_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1045123.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7875..8681)
                     /locus_tag="IW735_002138"
     CDS             complement(7875..8681)
                     /locus_tag="IW735_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045124.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8819..10333
                     /locus_tag="IW735_002139"
     CDS             8819..10333
                     /locus_tag="IW735_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1045125.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3694 a         1739 c         2048 g         2933 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tgcaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagatgctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa
     4861 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
     4921 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
     4981 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
     5041 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
     5101 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
     5161 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
     5221 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
     5281 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
     5341 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
     5401 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
     5461 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
     5521 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
     5581 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
     5641 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
     5701 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
     5761 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
     5821 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     5881 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     5941 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     6001 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     6061 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     6121 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     6181 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     6241 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     6301 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     6361 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     6421 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     6481 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     6541 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     6601 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     6661 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     6721 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     6781 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     6841 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     6901 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     6961 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     7021 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     7081 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     7141 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     7201 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     7261 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     7321 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     7381 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     7441 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     7501 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     7561 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     7621 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     7681 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     7741 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     7801 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     7861 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     7921 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     7981 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     8041 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     8101 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     8161 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     8221 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     8281 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     8341 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     8401 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     8461 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     8521 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     8581 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     8641 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     8701 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     8761 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     8821 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     8881 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     8941 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     9001 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     9061 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     9121 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     9181 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     9241 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     9301 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     9361 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     9421 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     9481 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     9541 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     9601 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     9661 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     9721 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     9781 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     9841 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     9901 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     9961 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
    10021 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
    10081 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
    10141 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
    10201 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
    10261 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
    10321 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
    10381 ataccgaggg tcagctgatt gaacactgtt tagt
//
LOCUS       DACEMS010000099         9873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEMS010000099 DACEMS010000000
VERSION     DACEMS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.102"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(22..267)
                     /locus_tag="IW735_002140"
     CDS             complement(22..267)
                     /locus_tag="IW735_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045126.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="IW735_002141"
     CDS             complement(381..959)
                     /locus_tag="IW735_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1045127.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="IW735_002142"
     CDS             complement(1036..1566)
                     /locus_tag="IW735_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1045128.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IW735_002143"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IW735_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1045129.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="IW735_002144"
     CDS             complement(2423..2818)
                     /locus_tag="IW735_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1045130.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="IW735_002145"
     CDS             complement(2815..4227)
                     /locus_tag="IW735_002145"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1045131.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IW735_002146"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IW735_002146"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1045132.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5082..6542)
                     /locus_tag="IW735_002147"
     CDS             complement(5082..6542)
                     /locus_tag="IW735_002147"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1045133.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="IW735_002148"
     CDS             complement(6712..7869)
                     /locus_tag="IW735_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1045134.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IW735_002149"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IW735_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1045135.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="IW735_002150"
     CDS             complement(9336..9863)
                     /locus_tag="IW735_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1045136.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DACEMS010000100         9698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEMS010000100 DACEMS010000000
VERSION     DACEMS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.103"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            118..540
                     /locus_tag="IW735_002151"
     CDS             118..540
                     /locus_tag="IW735_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1045137.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(706..1899)
                     /locus_tag="IW735_002152"
     CDS             complement(706..1899)
                     /locus_tag="IW735_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1045138.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2123..2500
                     /locus_tag="IW735_002153"
     CDS             2123..2500
                     /locus_tag="IW735_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1045139.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2488..2826
                     /locus_tag="IW735_002154"
     CDS             2488..2826
                     /locus_tag="IW735_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1045140.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            2960..3403
                     /locus_tag="IW735_002155"
     CDS             2960..3403
                     /locus_tag="IW735_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1045141.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3665..4480
                     /locus_tag="IW735_002156"
     CDS             3665..4480
                     /locus_tag="IW735_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1045142.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4490..5152
                     /locus_tag="IW735_002157"
     CDS             4490..5152
                     /locus_tag="IW735_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1045143.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            5357..7021
                     /gene="alsS"
                     /locus_tag="IW735_002158"
     CDS             5357..7021
                     /gene="alsS"
                     /locus_tag="IW735_002158"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1045144.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            7034..7744
                     /gene="budA"
                     /locus_tag="IW735_002159"
     CDS             7034..7744
                     /gene="budA"
                     /locus_tag="IW735_002159"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1045145.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7874..8371
                     /locus_tag="IW735_002160"
     CDS             7874..8371
                     /locus_tag="IW735_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1045146.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            8556..8816
                     /locus_tag="IW735_002161"
     CDS             8556..8816
                     /locus_tag="IW735_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045147.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(9178..9417)
                     /locus_tag="IW735_002162"
     CDS             complement(9178..9417)
                     /locus_tag="IW735_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045148.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         3286 a         1588 c         1934 g         2890 t
ORIGIN      
        1 aggaaaaaaa gagaaataat aacaggttta tgcactattg acggtgtgac aatatgatat
       61 ttgtatccgt ttttttcttg tatgtagtat agtaaagtag atacaggagg agaaaatatg
      121 cagacttttt taacaaaagt ttctcaaaag aaaattaaaa tgattcattt actgcttgaa
      181 acagaacgtt ggtgttcaat agaagaacta caaaacgaat tgaaggtatc aagtaagtct
      241 atcttgaatt atctaacaga tttagaagaa ttgttccaaa aatatcctga taaggtcatt
      301 ttgaagaatg agaataatcg gcgcttttct atggaaaagc aggagaactt cccaatctac
      361 attatttatc ttcatttcta cagaaaatca tataattatc atttgattga attcatgtat
      421 caacatcctg agaaaaattt ggaagattat gcagacgctc aatatacgag tgtatccaca
      481 gtttttcgtt atgctaagtt attagtaaag tactttgaaa tgacggacgt caaagaatag
      541 tagccgccag atagaaggaa attagcagtt gaaaaagctg ccctgcatca agtaatacga
      601 ccagccaaac ggaaacagtg tctcatgttt ttgatttggc tggtcttatt accgcagatg
      661 caagcttttg ccacacctct ttcttgtcat ttacagactc aactatcaat gctgcaagct
      721 ttcccttaat gctgtaacac cagtaagagt tgtcaagacc agcagactga ctaggtaaat
      781 accgctcaaa aacaagatta cggcagttaa actataatga tccatcaaga aaccgatgac
      841 gatagaagaa aaaccaccaa cagctcgtcc aaggttcata atgaaattat tcgccgtcgc
      901 gcggatttct gttggataca aactactaat cactgcgcca tatccaccat acatgccatt
      961 gataaagtag ccgactatca cagcggctgc taccaaactc cactggctat gggctaacac
     1021 aagtagatag acagataagg atgaacaaat taaaaacaaa gaaaaggcta gtttaggccc
     1081 caatttatcc atgattatgc caaaagtcat catccccaaa gacatgccta aaatagtact
     1141 gaccatccac aatgaagacc cagaaaccga aagccctaat cgattttgca taattgaagg
     1201 cagccaattc attaacccaa aatagccggc aatttggaca gttaccatga tactaaggcc
     1261 acatgtttgc caagcaagat gttttgattc aaacagtctg ctaagtttgg ttttcttttc
     1321 ggcagattgt acagatttga attcctttgg ttcagtcaag ccgcgacgaa tgaacaagac
     1381 catcaacacc ggcagtacac ctacgacata caacatcttc caaccaaacg ttggaatgat
     1441 cacagcagct aaaatcgcag ccaaagccgc accgacctgc gcaccaatac cagcaaccga
     1501 agaagctcgt ccaatttgtt tttttgaaaa actttcggaa atcaaggaca tacacgcccc
     1561 gtattctcct cccgctccta cacctgcgat aaagcgaaag agataaacca agtagatgtt
     1621 tgaagcaaaa tacatcaata aagaagcaat cgaaaacaga aggaccgttt gagaaaatac
     1681 tttcacacgt ccatatttat cagctatgat gccaaaaaaa atacctccaa tgagcatccc
     1741 caaattagtg atcgttgaaa tgaaacccgc ttgcgtacca ctaatatgaa aagaagtaat
     1801 cattgaagat aatgaaaaag ctaaaaacat aatatccata ttctctaaag caattcccgc
     1861 cgatgtcgat aataataact ttcgctgata cgttgtcatt tttatacccc ttttcctaaa
     1921 atttccctaa attgttaaaa gtatatccaa aaaagaataa aaaaacaaga cttttttatt
     1981 tgaaataata cacaaacaac cgatatagat aatccagctt agttttaata gaaatgagta
     2041 ttgacttgga gtacacttaa agttgtagaa taaataaaat acataagtaa agtggcaaga
     2101 aattaagaga aagagtgatc agatgaatat caaacaagtc agtgaagaaa agggcatttc
     2161 ggccgataca ttgcgctatt atgaacgaat cgggttgatt ccaccagtta atcggacaaa
     2221 aggcggaatc cgtgattata cagaagaaga cctaaaatgg gtcgacttta cgatctgtat
     2281 gcgaaatgcc ggattatcga tcgagtcttt gattgaatat attcgcctat ctagcgaagg
     2341 agaagcaact gtatttgagc ggcgtaagct tttgattgaa gaaagtgaac ttttgacgaa
     2401 aaaaattgct gaaatgcaag aatgtttgga acgtttacaa ggaaaaatca agaaatatga
     2461 tcgtgtgtta tctagaaata aaatggaatg tatcaagtaa aaaaatcaag gagcatcgct
     2521 atggagttac gcttactaaa atatttttgg acaattgcag aagaaggaac aatctctcaa
     2581 gccgcagaag ttctacatct tacacaacca accttaagcc gacaactgaa ggagttagaa
     2641 gaggaactag gaacagaact gtttgtacgc gaaaaccgca aaatgatctt gacagaagca
     2701 ggttattttc taaaaaagcc gagcagaaga aattttagat ttaacgaatc aaaccactca
     2761 agaattcgag aacagaaaaa aacaattatt cagcggccga tttacgatcg gctgtgtgga
     2821 agctgataat tcgaatacat tggcactatt actagaaaag ttgatccgtg attatcccag
     2881 cgttcaattt catatccaca gcggaacaag tgatgatatc acggagtgac tagaaaaaag
     2941 cctgttagat ttagcgattt tgctagatcc agtagataca gagaaatacg aaacgatttt
     3001 gttaccaaga aaggaaaaat ggggattgct cattttagaa aattctgttt tagcaaaaaa
     3061 agacgtgata acaaaagaag actttgcgaa tgtacctctt ttgatctcaa agcgtccaga
     3121 aatacagcga atgtttgctg actggttagg cataaaaaag gagcagttgc tgatcacagg
     3181 aacgtacaat ttgattttca atgtatttgc gctaattgag aatcaggtag gtgctgcttt
     3241 agtcatagaa ggtgctgtaa gtcatctttc aaagcgagtg acattcgttc cgcttgaccc
     3301 aatgattgaa acgaactgca tgctagtctg gaaaaagaat cggattttat cacctgtagt
     3361 caatgaattg attcaccgtt tcaaagaagc ttttgaacag taataagtat ctctaatcga
     3421 agaaatttca atacaaaagc gagacgtatg acagtcttgc ttttgtattt tttcattttt
     3481 aaatcaaaaa aaataaagag atttacataa cttgttaaat aagcgcttac aaacataaga
     3541 agaacggaaa tgaatcagct ataataaccg ttgttctgga ttgtaatcat tacaaagaaa
     3601 tgaagattat aactttggag gtgttcgaaa tgaaagaaaa aaagcaaaaa ttcgttcaaa
     3661 aaacatgccc tttattcaat ggttgggtat gattctgctg attgtattag ctttattgac
     3721 gatcattcct tttttatgga tggtttctgc ttcattcaaa gcaaataatg aagtgttcac
     3781 gattccgatt caatggatcc ctaaaacttg gcatttagag aattatctgg ttatttggtc
     3841 tcgtattcct ttagtgacat tttttaagaa tacagctttt ttaagcgttg tgatcacgtt
     3901 gattcagttg tttacttcta gttttgctgc ttacgggttt tctaaactaa attttaaagg
     3961 gcgcgatatt ttatttttag catatatcgg cacgattgct gtcccttggc agtcttatat
     4021 gatcccgcag ttcattatga tgagaaaagt gggtttaaca tatacgttgt ggtcgttggt
     4081 gctattacaa gcgttcaatg cttttggtgt gttcttgttg aagcaatact acagtagtat
     4141 cccagattct ttatgtgaga gtgcgcggat cgatggctta tcagagtggg gaatctatcg
     4201 caagatcatt ttacctctta caaaaccggc gattgccagt ttgacgatca tcacattcgt
     4261 taatacatgg aacgattata tggggccatt catttatttg tcttctacgg aaaataaaac
     4321 aatccagtta gggttgaaaa tgtttattgg gctatttgat gcagagtacg cattaatcat
     4381 ggctgcctca gttgtttctg ttcttcctgt tgcttttata tttttagcga tgcaaaaata
     4441 ttttgtagaa gggatcgctt catcaggaat gaaggaatag gaggtgatta tgaaagagca
     4501 gcgttttgac aaaaatgttt atatggaatt cttttattgg ttgtatcaat cattggcaag
     4561 tagcctttgt ttttggtgtg tcaatatctt gtatatagca gctgcgttcc ttttgtcgat
     4621 agatggaagg aatgtctttt cctttatttt ttctttactt tttattggtc caggttgcat
     4681 cgtgatgata ggtatgacag aagaattagc aaaagaaaaa agtttttcac cagtacgttt
     4741 gttcataaag acgtttcgcc aattttggct aaagggattt ttttattggc tatttgcttg
     4801 gatagttagt gtgataatga tttttgattg tttctttttt atacgttttt cttatggaaa
     4861 atggttgatt ccgttgtttg ttttacttgc ttgtttatca gtttccttta gcatcaacta
     4921 ctggtatttt caggtgagaa atccagcgtc gaaaccaaat caagttctac ggatagcgtt
     4981 ctactatacg ttgaaaaaat ggtatgtgag tttactagat tttttactat tgacaagttt
     5041 gtttctcttc tttttcgtaa agccccaatg gtgtattcta ctaggtcctt ctatcgtatt
     5101 tggtttgatt tacttcaaca atcgtaaatt gatgagaact atggatctgt agactttata
     5161 aattgaaact ttcaattaag aatgacagtt tttatgtaaa aactgtcatt ctttttcatt
     5221 ttttgaaaca tatggctaaa caaatgaaat aaaacgtgct atacttttga atgaaacagg
     5281 tttagaaact gtatcaatta tttgtgatta tttctaacta ataattaaaa ataaagcata
     5341 aaaaggacga tgttcaatgg acgaaaaaat gaaacaaggt tcgaaatctg tagtagaaag
     5401 tttgattaat catcatgttg attatgtatt cggtatacca ggtgcaaaaa tcgatggtgt
     5461 atttaatgaa ttagaggatc aaggtcctga attaatcgtg actcgtcacg aacaaaatgc
     5521 tgcatttatg gcacaagcaa tcggacgtat tacaggagag ccaggagtag ttatcgcaac
     5581 aagtggtcct ggtgctagta acttagctac aggtttggta acagcgacag cagaaggtga
     5641 cccagtttta gcaattgccg gacaagtaaa acgaagcgat ctattgaagt tgacccatca
     5701 aagcatggat aatgcagcac tgttccagcc aatcacaaaa tacagtgcag aaatccaaga
     5761 tccagaaaca atttcagaag tgattgccaa tgcttatcgt atggctaaga gttcaaaaaa
     5821 aggcgcgagc tttatcagta tcccgcaaga tgtagtggat gcacctgtcc aagggaatgt
     5881 catcaagcca ttgagcgatc ctaaattagg ttctgcttct gcagatgata tccgttacct
     5941 agctgaacgc attagagaag caaaactacc ggtactatta gtaggtatgc gaggatcaag
     6001 tgaaaaagaa acgttagcca tcaggcaatt agttggaaag acagcattgc ctgttgtaga
     6061 gacattccaa gcagctggag tgatctcacg tgaattagaa gcgcatttct ttggtcgtgt
     6121 gggtttattt agaaaccaac caggagatat gcttttgaaa cgaagtgatc tagttattgc
     6181 tattggctat gatccaatcg aatatgaagc aagaaactgg aatgcggaaa aagatgcgcg
     6241 gatcattgtg atcgatgaag cgccagctga aatcgatcct tttatgcagc ctgaaagaga
     6301 attgatcggt gatatctcag ccactcttga tttattgaca ggttcattag agcctcaaca
     6361 agtgtcagaa gatgcaaaag aatatttagc atctcttcaa gcagaattaa cggagcggga
     6421 cattgttcaa tcaaaaggag aagcaggtat cttgcatcct ttagaagtta ttaacacgct
     6481 tcagtcaaaa gtaacagatg atatgactgt taccgtagat gtaggtagtc attatatttg
     6541 gatggcacgc cactttagaa gttatgaacc tcgtcatttg ttattcagta atggtatgca
     6601 aacgcttggt gttgcattgc catgggcgat ttctgctgca ttggtgcgtc caaatacaca
     6661 aatcgtatca gtttccggcg atgggggatt tttattctct gcccaggatt tagaaacggc
     6721 ggtacgtaaa aaattgaata tcgttcattt gatttggaat gatggacact acaatatggt
     6781 agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt gtagactttg gtccagttga
     6841 ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc cgagctacaa gtgtagagga
     6901 attagaaaaa gctttagaag aaggatttgc gacagaagga cccgtaatta ttgatattcc
     6961 gattgattac cgtgataatg agaaattggg agaaacaatc ttaccagatc aattctatta
     7021 aggaagtgtc gacatgactg aaaaaatctt ataccagcac gggacattag gagctttgat
     7081 ggctggcttg atggatggaa cagaaacgat tgctcatata ttagaaaaag gaactctggg
     7141 attgggaaca ttgcatgggc ttgatggtga agtcatcttt ttagatggcg tagcttatca
     7201 aggaagatca gatggctcag ttgcccaatt agatggaagt gaacttaccc cctatgcagc
     7261 aattacagat tttacgccag atactagttt ttctgtctca aaaactgcag atggagaaca
     7321 attgaaaaaa gacatattag taagagaagc cggggaaaac ctatttttgg cagtgaagat
     7381 aactggatta ttcaaaaata tgcacatccg tatcatgccg aagcaagaaa aaccttacag
     7441 aagactagca aaaatttcag agtctcaacc agaattccaa caaagcaatg tccaaggaac
     7501 attagtcggg ttctttacgc ctgaattatt ccaaggagtt gctgcagcgg gatttcactt
     7561 gcattttatc gatgatacac atacatttgg cggacatgta atggattttg aagtggctga
     7621 aggaaaagtt gagattagcc gcatctcgtc ccttgtacag cattttccag ttgatgatcc
     7681 gacttttgtt cacgaaacga ttgattatgc tgatttagca gcagaaatcc aacaagcaga
     7741 atagaaaaag acagcgagat gcgttcaagg tgattaacct gacgtatctc gtttttttga
     7801 ggaggaaacc tagcgtttac aaaaaaactg agctataatc ttctccatag atcaaaaagg
     7861 agcggttttt atcatgtgga cagaaaaaca gaaaaaagat gcatacgaaa ttttattatt
     7921 acagcaacaa ggattattag aagcagaaga taactggtta gcaaatttag caaattcatc
     7981 tgcattgttg aatgagactt tgcctgagac tgtctttgca ggatattatt tattcgatgg
     8041 taatgaattg attttaggac cgttccaagg gcgagtttct tgcacacgta tcaagatggg
     8101 aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca ttgatcgttg atgatgtcaa
     8161 aacccatgca aattacattt cctgcgattc agccgcacaa tcagaaatcg tagtgccaat
     8221 ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat tgcggagtaa cgaaagggta
     8281 tgatgaaatc gatcaagcat ttctagaaaa atatgtagat cttttattag caaattttaa
     8341 tataaatgct ttttcattta ttaataacta aaatcgtcta ttgaaacaaa aaagaagaga
     8401 aatcaagaat aaaaagagcc ggaaagacag tgggcatttc tgtttttcgg tttttttatt
     8461 tttttataaa atcacaagtg ttattgattt tcataatcat attttaatgg tattatcatt
     8521 ttaaataatg gaaaaacatt aaggatggat gaaatatgaa taacgaaact atcattttag
     8581 gaaaaaaata tatttgccaa cctattggct tgaaacaaaa agtggttgga gaagtaatca
     8641 gtaaattaga aaattgtgtt gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg
     8701 aaatattaga aaaatgcgga aaagtagtgg caaaatatac tcacgtttat gagttggaaa
     8761 caacagaata tctatataac tatagcaaca aatatgaaca tactttagtt tcataaagat
     8821 ttaaataaag tgccttcaaa agcgagaaga ccaagctttt gaaggcactt tatttagttg
     8881 gcagtgattg ttcatgattt ttgatttatt caaaaggttc gcctagagaa tctgtctctg
     8941 taacgaggcc tggtggagaa actcgataaa aacctatact gccagaactg tcctattttc
     9001 ggatagacaa actagtagtt ttaaaaagat catccgatcc aatcatttca aaaaatgcaa
     9061 aactgatatc aggatcattt agctcaggct tgttatcgta aagcatttgt aaaggttgca
     9121 tttagttagg agtaaacgcg aaactatcat cattatttgc tgaagaagat gaagaagcta
     9181 attcagagtt tgtttgaaat aaaggagacg tgctgacagt ttctttaccc tgttttttgc
     9241 agaggtagaa acttttatat cggaagtttc taccggagaa tttttctcat ttgtagaaat
     9301 tgttgaatcg atactattcg aaatttgcgt atctgaaggt atgttctttg atgcagaagt
     9361 cccacaagca gtaagtaaca ataaatttga taaactgata aacagaattc ttttcattat
     9421 gtacacactc cttatagtat cgtttagcat acacctatgc tcagtattag gcaaaatcaa
     9481 tgcaaaacta tcaagtcttt tatagtatct attagtctta gtaatacata tatggagagg
     9541 gtgatatccc agttcaaacc agatacattg gttaccatct gtattaacga ttatgcctct
     9601 aaacgctttt tttatgaaaa aatttataag tgaaaacgta ggtatgatat tatctagagg
     9661 taattttttt gggtatttaa atagttgcta ggaggata
//
LOCUS       DACEMS010000101         9602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEMS010000101 DACEMS010000000
VERSION     DACEMS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.104"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(244..1608)
                     /locus_tag="IW735_002163"
     CDS             complement(244..1608)
                     /locus_tag="IW735_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1045149.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1605..3164)
                     /locus_tag="IW735_002164"
     CDS             complement(1605..3164)
                     /locus_tag="IW735_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1045150.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3220..5700)
                     /locus_tag="IW735_002165"
     CDS             complement(3220..5700)
                     /locus_tag="IW735_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045151.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5684..6019)
                     /locus_tag="IW735_002166"
     CDS             complement(5684..6019)
                     /locus_tag="IW735_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045152.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(6033..7067)
                     /locus_tag="IW735_002167"
     CDS             complement(6033..7067)
                     /locus_tag="IW735_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1045153.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(7157..7837)
                     /locus_tag="IW735_002168"
     CDS             complement(7157..7837)
                     /locus_tag="IW735_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1045154.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(8121..9224)
                     /locus_tag="IW735_002169"
     CDS             complement(8121..9224)
                     /locus_tag="IW735_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1045155.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         2776 a         1957 c         1688 g         3181 t
ORIGIN      
        1 tcaaaaacat cacccctgca actaacgcat aacgtaactt tgaatgcata atttcattta
       61 atgctaaaaa catttaactc ctcctcctga aataatcatt ttatacaaca attttatcac
      121 ttgataaatt tttggcgaat attatgctca aaaaattctt ccgctatttc attatatgaa
      181 ttttttattc tcgattgatt aatatttatt atatccccac acaataaaag accgtgatat
      241 ttctcacagt cttttatcta ctctattgct atttgctctc ttagctagta ctttaaaagc
      301 taactcattg aggtagtgaa aacgagaggt gtgttcagac agaaatccaa aatcctcaac
      361 tgctagctgt ggaatcgtag ttttgtgttt cacgatatcc gcaagcaggt tcaaatctga
      421 taataagaat tcttggctta tcagctgtcc gccaattatt tctaatgtat ccggatggta
      481 gatcagcttt gcatttactg ttttgttttc ttcatcgtat tgtggaaata gatcaaattc
      541 tcctgaacac gaaccggtat tttgttccaa gaatggcgct tcatcttctg tcagccctac
      601 agttcctaaa aagtagtcta taatgactgc tgctgttatc ttttgtctga ggtcgtaagc
      661 ggttggctga ccactcaaag ttagcgctac tgctcttgcc atatgtacag ctcgagtaac
      721 taaaggtaaa aagagatgcc gtttattttt gtcaaaactg actgggacga tatctccaat
      781 cgcatagatg tccggatcac ttgtctgcag atattcattg actatcacag tctcatcctc
      841 atacaaagcc actttttctt ttaacaatgt cgtattagga cgagtttggg cagaaaaaat
      901 gaccatatct gcctttattg cttgagatag aagttgtacg gatactactt tctcttctga
      961 ttttttccaa tcgatcaaga attcatttgg ataaaaattg atttgcttag tttgcagctg
     1021 attttgaaaa ctgttgatca tatccttatc gaaatatctt gataaaacat tcggcatata
     1081 atcaactaga tgaataatgt atcctttatt tctcaaagca tctgctaatt ccaatccaat
     1141 ataaccagca ccgaagatca ctacttcttt tgcttctttt aaagcggcaa gtacttgctt
     1201 agattgcact agatttttat aggtgtagac attttcagct tcaatttgtt tatacaaggt
     1261 gacaggagag gaacctgttg ctacgattaa tttatcatag gttatggtct cgttttttcc
     1321 ttctttggat gcgaggatga gctgatgttt gtctggatca agttcagtta ctctccattc
     1381 ggtgatcagt tttgccccac tttcttgaag agtccgcatg ttatgggcaa cttcagctaa
     1441 actacgtatc tttcctttta aataaaggtt gattccattt gaaacatagc ctagtccatc
     1501 tttatgatct tcatcgatca gtaaaacttc tgctttcggt tgaagttttc ttagatttag
     1561 ggcagctgtg atgcctgcat gggaagcacc aatgatcacg attttcatgt tttattcatc
     1621 cttaaattca atttttctaa ataacattct tttaaaaatt ggttcaaata ggggaaatca
     1681 taattatatt cgttgctagt gaatacaaaa actttagcgg acttctgtga cgggtactct
     1741 ttcaattggc tagtcaaaat gatatcatac tgcttttctt tctcgtattc gactaaatca
     1801 tatttgatcg tattcagcaa ttgttcctgg atgatttctt tcacgatatc cgccattagc
     1861 ctttcataag gaagatcata agcaatttgc agacgtaatt ctaactgctt gatcagcgtc
     1921 cgcaaaatag atgcatagcg agcaaataat tcaaagcggg cattttcctc gtctacttca
     1981 gtaaaatcaa caatagcaaa tgtttgtctg accaatcttt cacataaaac caggaattgt
     2041 ttatcttcta cttcagttaa ccgctgtttt aatccttctt ggccaaaaat attcatccat
     2101 cctctaaaat aggcaaaatg agcgtgattt tgcatcactg tcaacgcaat cttttgcgaa
     2161 atctcttctg gaaaaacata aaaagagaaa tattcttcta tcgattgttt aaacgttcga
     2221 ttcaattgag ctactacgtc gtctttgtat ttttcgctta tcaaaaactc ctggacatgc
     2281 gagttatgtg tatctaatgt aaagttactg atcatgaaaa gataaaaaac caacgtttca
     2341 ttttgattcc aaggaaaggc atattgaaag aagtaacggg tcaaaaatgt atggaggtcc
     2401 aataaaaatt gtgtcgtttg aaacaatcca acaggaagat tgctataatc tggtttggat
     2461 tttttcgtac agcgcattcg cttcttcata atggaaagcc atatttcaaa tttcaatcgt
     2521 ccttcttctg taatagtaaa actaaactct ttttgcagca gatctaaaaa agtgtttcct
     2581 gataaatcac tgtattcttt tttgttttca agataaggac gtccaaacca aaataattgg
     2641 aaataaaaat aacaaatttg actttctgga ccggtcagcc gcccgctttt tattttcaga
     2701 tgaaactcat ttaaagaatc atttaattcc ttcactctgc gagacaaagt agccgaactg
     2761 atattaagtt ccatgacaag tttctgccga tcaaatgagc cattttccaa cagataaaga
     2821 agtatctgat attttgtaga atagcgcaag tagcaatgat aataaacatc gactgctgct
     2881 atatttccat gtctgattaa taaaatattc ccttcttgga taacaatttc caattgtttg
     2941 atattttcca agttagcttg taaataaaac agatcttctt tcaaggtagc ccgatcaact
     3001 gataagcggg taagtacgtt attaacggaa atctctcctt tttcctctaa taaaagttgg
     3061 aaaagtttga tttgccgttg ttcatttttt tcaagcaaac tgaaaagttc caagtccact
     3121 cctccaagca tttctgattt taacagcaga agtttccttc tcatccttga aaaagaggat
     3181 ctgtgacagt tatcacagat ccatacaaat tctaatcact tagctggttg gcccagctgt
     3241 cagttcccat gtgaccatgc cttgatacgt tgcgccaggt tgagcattcg tactgctatt
     3301 tacctctaag agtaaaccat cggtatcggg aaagtcaacg agaacctctc ctttagctgt
     3361 tccatcactc tcataaatca acgtgttttg accttgctga atcaattgtt ttgatccaga
     3421 atgattgaag tataattttg ttccgcttaa ttcttgacct tgatcattct taaataaaga
     3481 agtcaatgtc cctgtcagtt gccaaggaga cgtgcttgga agccgagtat cccgcacatc
     3541 aaaagaaaaa tcgccttttg cttcataata acttgtcttc aacggtttgg ggagatcgtt
     3601 accaaatgta aaatcatctg gtacattaac taatgttaat tcttgtttgg ggacaagaat
     3661 tgttttagca gatgtttgga caactgggtt aggaagctta gcaccatctg ctaatgtaat
     3721 attgctggaa agtacagctg cctttgtcag tatgctgacg gattgtacag aagtatctac
     3781 agtatttaat tgatagttgt aagtaagatg aatggtatct cctattttgc tcaatagatc
     3841 tgctgggaca gaaactcctt gttgaatgcc ggtatctgcg atagttgtac catttaattt
     3901 taaggaacca ttgatataag tagtattttc aggaagtgag atcgtgattt ttttgtcggt
     3961 tatcgcatcc ccagattttg ttaacgtgaa tgtcacattt tcttgaatcg tatctcctga
     4021 tagagcagac aggggatctc ctgtctcagg ttgtctgcta aggtttttta gctgactatc
     4081 tgcactcaca gatggaaaat tagccactgt tatttgagga agagtggtga ttccagcttg
     4141 ttcagactgc catttgtacg caaaagacgg atacatgtta gtaagtacat cgtccaccaa
     4201 gcgataatca aaataatatt gccctgaagc caatgcaggt agtgtagaag tagctgtatt
     4261 tacacccccg ttatttgatt ggatcgtttg gaaaagttga tctgtccatc ctgtcgctaa
     4321 tttttgggtt cctttcgaat aaagcctgta atggatcgaa ccctttttta gttcagttgc
     4381 tgaccaattg gccgtgactt ggtacgatgc agaagaaagg agctttcccc actgactatc
     4441 ggctgttaag tttgttggta aaaccgttcc gtctagcttg tactgctcca atacctttaa
     4501 agcaacagga gaaagcaatc cgccttgcgc atctttccca ctgtacacga tattggctgt
     4561 ctgattcaat ccacttgtgc ttgctggagt atgagtcagt attgctcctt gtgtaacgtt
     4621 ttttcctgta aaatagttga ctacgcgatc aaagcgacta aatggcaact gatcaaaacg
     4681 tccttcagtc agccccatcg tcaatgtccg actattggta gagcctggaa ggttctggtt
     4741 atttccatcc acgtattctg tggtatcagc aaatgtatta tattttcctg ttgaatcact
     4801 tgttgagatg taactattcc acgtatttat ctctgcactt gcagaaaatg catatgctcc
     4861 tggattcaag tttccgccag ttaatttagg ataaaatcta cctgcagctg gagctaaaat
     4921 cgaattgaac atacgatctt ggataatatt attcgaatca taaaaatcaa cagaaaaaac
     4981 ggccttttta gacatagaag gttgtaattt acccttggtc aaaattttgt ttggactcgt
     5041 atcatattta tctccgtttt ctgtcatctt cgcgacatta gctgtaacat caaattgaag
     5101 cgggatatcg tccacgttga attcgcctcg cgtaataata gcaataatat ctgccaatag
     5161 agtatttgta tcccacgacc ccatcccaaa acgcaaggta tggcttcctg ctgataataa
     5221 actaccgcta gtaaccttga tgggggcacc ggcaatatac aagtaggcgt tgttccagtc
     5281 aattgctttt aatacatcac tgcttgatac atgttccggc aaattgatga tcgccacaaa
     5341 tgcaaatcct tgtgcagtga tattcaccaa atcaagtaca tttgttagtt tcacatggct
     5401 ttcccaatga aaacggaccg tgccaccttt ccaaggatca tttgaaccat cgttcaaata
     5461 agtagcagtc aatttaggca attggttagc cccagttagt tggacaagct tcccgccatt
     5521 gactgcacta cgtccaccat ttggaggtac gatattttca tacgtgtatg gatcataaga
     5581 aatggcagga ggcgttgctc caacagcttc tgccgaaggt gaaaagataa atatacttaa
     5641 aacaaaaagc gaagtaaaaa agaaggagcg aaaaaatgaa aatttatttt gtagcaacat
     5701 gttcatcctc cttttccttt cttctccata acaacaacca cgcaagaagc cataattcta
     5761 taccaatgat caaataaaga aaacggatct tttcttcatt cgtctccggg aatacgccat
     5821 gttgattaga ttctttataa atcggtagct gtgtctgctg ttttgatgcg ccattgactg
     5881 aaccacttgg atgcggaact ggacttagat cataaaatgt aatcgtacct gaacttttgg
     5941 tatctgcttg gactggctct tgccagctga agaacagcaa acttgcaata atccctccca
     6001 ataagatcga tgttttcaac gaaatcacct catcatgttg tttctccttt tttctttttc
     6061 ttgtggaccc acaaaaacag caaaatcaac cataacacta gaactgcagc aaccaatcct
     6121 gcataaagcc atgggaaaga ttgttcttgt actgcaaaaa cggaattttc atttactttt
     6181 tgtgcttttt cttttttgat cgtaaagttt tgtgtaaatc gccattcatt actgtcgcct
     6241 gattttgcta tgatttctgc gcggtaatcg ccggcttttg ctttatcacc agctaaatca
     6301 agattcaagt gaaaattcga atttggtgca actaaaaatt cattagacgt attttcaata
     6361 taaagctgat tttcataaaa gatcttgctc gtaaatatca aattgctgat aatcctaggc
     6421 tgaggatttt ggaaacgaag ctgaacagtt ggctttgcgt tgacctcgtc caaatctcct
     6481 gataaaagtt tcagatccgg ttctatcgtc tcttgacttt cttgtaaaac gactgcgaga
     6541 ttataagcaa tacgattttt gatttgtgct ccttctgtag cattttctgt ttcagcttct
     6601 tctttaggtg aaacatgaat acccgctaat acacgtccct taaagctttt tgctgggatt
     6661 ttcacttcta taggtatagt tgtttctgca tgtgctgata cattgatttc ctttttcgca
     6721 atgagcaaaa cgtcacggac atcaaacggc aatgtctcat ctaacgttgg gtttttttga
     6781 ctataatcga tcgttccacc atcactcgtt accgccggat tgacagagac ctcaaatgtt
     6841 cgttcttcat ctgaagaatt gctgataatt acttctagtg tctgattttg ttccggtgcc
     6901 actaataaat caaaataacc gattgcttta tctatttggt tctctggaaa taccggtttg
     6961 attccaaagt ctcctgctga atcctttgct tggctgattg acggaaaaat gaaaaataga
     7021 cttaaaatag aaaaaaacga aacaaacagt cgtttacttt gattcattgt ccaccacatc
     7081 ctttcaacaa caactgcggt aaaaaatcct tcacctatag tattttcacc gcagtttgtt
     7141 gaatttattt catactctat gctactgggc cagccacgag gttccaagtc aaatcagccg
     7201 tgaatgttcc agctgctgct ttcggcactt tcagtgtgat atttgctgga tcataaacag
     7261 aaagccatgt tcccagacct tgatcttttg ctgctgtaaa tattggtgtc gctgcacttg
     7321 ctttactcaa cccagttaca gtttgactag ttggtccagt ggattcgtta tctgacgtac
     7381 ttttaacagt ggatgttcca aaatctaatt cagcgccttg caaaacttgt gaaccgttta
     7441 caaaatctga aaccgaaact gtcacgttcc acccttgtgc ttggccatct gcacctacac
     7501 ctcgacgatc tgatacttgg agataaggtg tatcgttttt tgaagcgtct acttgatacg
     7561 tttttgttcc gctttcaatc tcgtgtgtac caaatggcaa ttcaggaact acatccaatg
     7621 ttaaagaacc tgtctggcca gttgctggat tttccggatc tgctggctct actggttttg
     7681 aaggatcagt agggttaaga ggctcaacgg tatttgttgg ttcagtaaag gtaatatctc
     7741 cttttgaagt cgcttgaccc tcagcaaaag ctggtgttgc caagccgcct agaagaagtg
     7801 ttgaaagtaa taatgtccca atcatgttct ttttcataat aaatcactcc tttttcttag
     7861 tgacctaagt atagtagaga cagtatgcaa aaaatcctca tgaattcatc cgcttacttg
     7921 aaattatgac cctttttgta taaaagagaa aaaaacaata aaatcgttta catgaatatt
     7981 cttccttttt tattaagtat gaaaaaatat ttcaatttta ttttttcgtt tcaaattatt
     8041 ctattagtga catccttgaa ctataaaaat aggagtacgg catcatctgc cgcactccta
     8101 tttttttcgt catttatttc ttaaataaaa cgattctcac gcaattttat ttttttgata
     8161 tcttctactg tttgtgttcc agctaattgc atgaccattt ccaattctgt tttaaagaaa
     8221 tcaaacactt gatggactcc tgtacttcct cctaaagaca atccataaat aacgggtctg
     8281 ccaatagcaa caagatcggc acctgatgcg attgctttga aaacatgttg tccacgtcgt
     8341 acaccgctgt caaacacgat tggtacacgt ttatctactg cttctgccac atattgcaac
     8401 gaatcaaacg cagcaggccc cccatctaat tgacggccac catggttaga tacccaaatt
     8461 ccaccagcac cagattctaa tgatcgatag acatcttctt ctgattgaac acctttcacg
     8521 tagactggca aatcagaatg agcagcgata aattctacat cttttggact aagtttttgt
     8581 ttggaagatt tatatacagc atccatcgtt tggcctactc ctgattgata agcctgaacg
     8641 attggcattg gcagcgggaa ggtaaacccg tttctgcggt ctgtttcacg gtttccgcca
     8701 actgttgcat ccgctgtcaa tacgattgct tttgctccat tacgcttggc cacttcaaga
     8761 atatccaaat tgattccatt atctttgctc atataaaatt gaaaccattg cggtgctttc
     8821 tccccgcctg cttctcttat ctcttctaaa gtacaagaag cgtaagaact tgctgtatag
     8881 atcgtcccaa aatctgctac accttttgca gaagctttct ccgccttcac atgagctaat
     8941 ccatgagcag caactggtgc catgatgatt ggtgcagtca gcatttcttc gtcaaaatgt
     9001 gtagttgtat cgggtaattc tacatctctt aatacatgag gaataatcaa acgatgattg
     9061 aatgcccgtt cattttcttg ataagtaaat aagtcgccag caccactgct gatgtaacca
     9121 taaccaccag ctggaatcac ttgtgaagcc gcgatttcta aatcttccat attgataaaa
     9181 tctactattc cttcatttgt acctgcttga tatgtttttt ccatctcaat gccttctctc
     9241 tataaaattt gaatgatgaa caatatgata gcgtttacac tttttatatt actcttcgac
     9301 attgatacag gcaaataatc tgtttacatt tcagccattc cagatgtcac tactctggaa
     9361 gaaatgattg taataaaaat agataaaaaa ccgatgggac tttgacaaaa gtagtgtcaa
     9421 agtccctttt tttgaataaa cggtgttcaa aagccatttt ctcggtatta agtcaaattt
     9481 atgcaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa actaagcgac
     9541 tttttcacaa cctctcgata aaacggtgtt caatcagctg accctcggta ttagctcaaa
     9601 aa
//
LOCUS       DACEMS010000102         9394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEMS010000102 DACEMS010000000
VERSION     DACEMS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.105"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            236..1042
                     /locus_tag="IW735_002170"
     CDS             236..1042
                     /locus_tag="IW735_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1045156.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="IW735_002171"
     CDS             1054..2274
                     /locus_tag="IW735_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1045157.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="IW735_002172"
     CDS             2264..3850
                     /locus_tag="IW735_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1045158.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="IW735_002173"
     CDS             3889..6027
                     /locus_tag="IW735_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1045159.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="IW735_002174"
     CDS             6315..7706
                     /locus_tag="IW735_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1045160.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="IW735_002175"
     CDS             7765..8574
                     /locus_tag="IW735_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1045161.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..>9394
                     /locus_tag="IW735_002176"
     CDS             8546..>9394
                     /locus_tag="IW735_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1045162.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQ"
BASE COUNT         3238 a         1442 c         1896 g         2818 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg caaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa gaaaagcaaa aatattataa tcag
//
LOCUS       DACEMS010000103         9081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEMS010000103 DACEMS010000000
VERSION     DACEMS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.106"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            41..1177
                     /locus_tag="IW735_002177"
     CDS             41..1177
                     /locus_tag="IW735_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ1045163.1"
                     /translation="METTEAVQDDQDLAKMYTPGVAELSLMIEKNHALARELTISGKL
                     VAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAIPLAIEQKSVDEIVQTIV
                     NLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEGTAIVVLAGLINAAKIQR
                     KTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRLKKQDSTLNKYQRELLDH
                     IHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDNPIIFALANPVPEITPDL
                     AKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKVDLSLQLEVAKQLAAKVA
                     EPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAENIK"
     gene            1158..2462
                     /locus_tag="IW735_002178"
     CDS             1158..2462
                     /locus_tag="IW735_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1045164.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2488..3501
                     /gene="citC"
                     /locus_tag="IW735_002179"
     CDS             2488..3501
                     /gene="citC"
                     /locus_tag="IW735_002179"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ1045165.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3505..3798
                     /gene="citD"
                     /locus_tag="IW735_002180"
     CDS             3505..3798
                     /gene="citD"
                     /locus_tag="IW735_002180"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ1045166.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3803..4708
                     /gene="citE"
                     /locus_tag="IW735_002181"
     CDS             3803..4708
                     /gene="citE"
                     /locus_tag="IW735_002181"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ1045167.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4701..6260
                     /gene="citF"
                     /locus_tag="IW735_002182"
     CDS             4701..6260
                     /gene="citF"
                     /locus_tag="IW735_002182"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ1045168.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6257..6814
                     /gene="citX"
                     /locus_tag="IW735_002183"
     CDS             6257..6814
                     /gene="citX"
                     /locus_tag="IW735_002183"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ1045169.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6807..7670
                     /gene="citG"
                     /locus_tag="IW735_002184"
     CDS             6807..7670
                     /gene="citG"
                     /locus_tag="IW735_002184"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ1045170.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            7904..8313
                     /gene="tnpA"
                     /locus_tag="IW735_002185"
                     /pseudo
     CDS             7904..8313
                     /gene="tnpA"
                     /locus_tag="IW735_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(8950..>9081)
                     /locus_tag="IW735_002186"
     CDS             complement(8950..>9081)
                     /locus_tag="IW735_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1045171.1"
                     /translation="RRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3163 a         1419 c         1839 g         2660 t
ORIGIN      
        1 agttttagaa atccataaaa aaaatgtagg tgttttacgg atggaaacaa cagaagccgt
       61 gcaagatgat caagatttag caaaaatgta caccccaggt gtagcagaac taagtttaat
      121 gattgagaaa aatcatgctt tggcaagaga attaacaatt agtggtaaat tagtggccgt
      181 tattacagac gggtcggcgg tattaggtct tggaaatgta ggaaaccaag ctggattacc
      241 aatcgtcgaa gggaaagcgc tgctttataa aaatttagca ggtgtcaatg cgattccatt
      301 agccatcgaa caaaaatcag tagacgagat tgtacaaaca attgtcaatt tgcaaaatag
      361 ttttgcaggg atccatttag aagatattgc agcaccgaag tgttttgaga ttgaagaaaa
      421 attacaagaa aaactttcta ttcctgtgta tcatgacgat caagaaggaa cagcgatagt
      481 tgttttagct ggattgatca atgctgctaa gatccaaagg aaaacattaa ctgaacttcg
      541 agttttgatc aacggcatgg gagcatctgg ggttgcaaca gctagactat taatcgctgc
      601 tggtatcaaa aatctaacat tggtggataa acaagggaga ttaaagaagc aagatagtac
      661 attaaataaa tatcaaagag agttgttgga tcatatacat gatacaaata ataatgcaag
      721 cgttttggaa gatgcaatcg ttggacaaga tgtttttatc ggtctttcaa taggaaatgt
      781 tttaacgcct gaaatgattg gaaaaatgaa tgataatccg attatttttg ctttagccaa
      841 tccagtacca gagatcacac ccgatttagc aaaagaatct ggtgcaaagc taatcgctac
      901 aggttcttcg aaatatccta accaagtaaa caatatttta gtatttcccg gattgttcaa
      961 gggactatta ctatcgaaag cgaataaagt cgatctttca ttacaacttg aagtagccaa
     1021 acaattagct gctaaggtcg cagagcctac ggtagaaaaa ttcatcccta atgtttttga
     1081 tgaaggagtc gcaaacgtcg tttcacaggc agttttggat tatttacaaa aactaaaaga
     1141 aaacgaggat aatatacatg ctgaaaacat taaatagaat taaaattggt tttattcctt
     1201 tatctcttta tatcattttg gtactcgcgt atcttgcaat gacgataagc aatatcgttc
     1261 cagaaaatga tatgttagtt tctttgggta ttatgactgt ttttgcttat ttattagaag
     1321 aaattggtaa aactattcct attttaaaag atttaggtgg taaagtatta gttgttacat
     1381 ttttgcctgc atttctagtc tataaacatt ggctacctca aagtactgta actgtggttt
     1441 ctgattttat gaataataac aatttcttga tgttctttat cacactacta gttgtcggca
     1501 gtattgcttc aatgaatcgg caaacattgg ttaaagcaac ttcacgtatt atcattattt
     1561 taattttatg cgatgtgatt ggttctttga taggaacagg tgtgggaata ctccttgggc
     1621 tatctgcttg gaatgcgtat ttctttatcg ttgcgccgat tatggctggt ggtgttgggg
     1681 aaggagcgat gccattatcg attggttatg cagcaatatt atctggtatg ggacaaaatg
     1741 atatctttgg gaagattcta ccgtgtgtga tgttgggaag tttagtcgca gttctactgt
     1801 ctggtatgct caaaaaaatt ggagagaaat acccacaatt aaatggtcag ggtaatttga
     1861 tcgatgggga tgatgaacat ttagagaact tgaagggttc agctaaacca gtcaatattg
     1921 aaaaaacatt gttagcaggc gtggtagcaa taacgattta ttttgttgct gtctatgttg
     1981 agcacttatt aaaatcactc cttggctttt ctattccttc acctgtgatc ttattagtct
     2041 ctcttatggg ggctaaaatg cttggtttta tccctaaaaa tatcgaagaa ggtggaaatt
     2101 cgttgtatgg attcactgtt cgtgggatca cacctccctt actttttggc gtaggggttg
     2161 caaaaacaga ttggttagag ctgatcgaag tatttaaaaa tcctgcaacg atagtaacaa
     2221 tcattgtgac ggtagtagct attgtcgcta cagcttttat cgccgcaaaa tttttaaaaa
     2281 tgtacccagt tgatactgcc atcgcagttt catgttgtag cggacaaggc ggtacaggtg
     2341 ctttagctat cttagctgcg ggtgatcgca tggaattaat gccatttgca caagttgctg
     2401 ttcgactagg gggtgctatg accgtaacgt tcgctatctt tttaatgggt ctgctatcat
     2461 aagaacataa agaaaggacg tgaccatgtg tttgatcaaa cagatatcca acgattattt
     2521 ttaaaaaata actatatcaa agaccaatgg gaaaatcttt tgcttgattc aggaatacaa
     2581 aagaagcaaa tcgaagattt tcaacattta gatcaaacat tgggtatata ccataaagaa
     2641 aagttagtag gaacagtatc ttatcaaaac aacgtgatta aatatatcgc cgtgtctgaa
     2701 aaatataaag atggtggaca tacatttaat gtactagtct ctgcaataat tcaggcaatg
     2761 agtcaacaag agatttttca ttatattgtt ttcacgaaac cgcgctatca aaaaagtttt
     2821 aactttttag gttttgaaac aatcgtatct accccaaaaa tagctgtatt agaaatggga
     2881 gatagttcta taaaacagta cctagagagg attcctaaag tgaatggtga aaaaaaagca
     2941 gcaattgtca tgaatgcgaa cccatttaca gaagggcatt attatttagt aaaacaagct
     3001 gccaataaca acgattgggt atatgtattt gtactcgaaa aagaacaaaa tcttttttcg
     3061 acgaaggacc gactcaaatt agttcaacaa ggattacaaa aactgaaaaa tgtcattgtt
     3121 ctatcaggtg gcgagtatat tattagccct gcaacgtttc ccacctattt tttacgagaa
     3181 gatgatgaaa tagcaaagga gcaaatggtc gtagatgcta ctttattcaa agaaagaatt
     3241 gccacttatt tagatatctc tgctagatat gtgggagaag aaccactttc cccaatgact
     3301 cgttcatata acgacatact ggaaacgatt ttaccccctg aaataaaagt tcatgtcctt
     3361 gcaaggaaga aaacggaaca acaccaagtt atttctgctt ctcaagttcg taaagcgtat
     3421 cttgctgggc aattggaaaa aataaaatat atggttccag aaacaaccta tcaatactta
     3481 aaaaataaaa gggagcgtta agtaatggaa ataaataaat cagcgattgc tggaacatta
     3541 gagtcaagtg atgtccaaat catgatttca ccgaatacag aaggaataga aatcgattta
     3601 gacagtcaag tgattgagca atttggaaaa caaatcaaaa aagttatcta tgacacacta
     3661 gcagtttatc agattaaaaa cgtaaaactc aaggtagtag ataaaggggc attagattgc
     3721 gttattgttg cacgattaaa aacagcgatc catcgttcct taggtatcca acaccaagaa
     3781 acaccgtggg aggtttaatt agatgatgaa agaacgtttg agaagaacaa tgatgtttgt
     3841 tccaggaaat aatccagcga tgatcaaaga tgcaggtatt tatggtgctg actcgattat
     3901 gttcgacctg gaagatgcag tgtctatggc agaaaaagac agcgcaagaa tgcttgtggc
     3961 tgaggcattg aaaacagtgg attatggtga cacagaatta attgttcgaa taaatgacct
     4021 gcactcagaa tttggacgtg cagacatttt tgcaatggtt aaagccggcg cgcatgtatt
     4081 acgtttacct aaaacagaaa cctctgaaga tataacagaa gtagaaaaag tgattataga
     4141 agcagaaaac tatcataatg ttccgatagg gcgaacattg atgatggcag ccattgaagg
     4201 tgcttctggt gtattgaacg cacctgcgat cgccaaagca tctgatcgat tgattggaat
     4261 cgccattggg gcagaagact atgtgacaaa tatgaaaacc aaacgatatc cagatggaaa
     4321 cgaattgttt tttgctcgca gtatgatcgt tcatgctgca cgtgcagcag gcatttccgc
     4381 atttgacact gtttactctg atgtggatga tatggaagga tttttaaacg aagtacgttt
     4441 aatccatcaa ttaggttttg atggaaaatc ttgtatcaat ccacggcaaa ttccagtagt
     4501 caatactgtt tatcagccgt caatcaaaga aatcaaacga tcactagaca tcatctcggc
     4561 aatcgaagaa gcaaaagaaa aaggttctgg cgtaatttct ttaaatggaa aaatgatcga
     4621 taaaccaatt gtagaacgtg cacaacggac attgatgtta gcaaaagcat cacgattgat
     4681 tgatgaggag ggaaatatcc atgcataata aacaaagacg tagcattcca tcaagtgttt
     4741 taacaaagta taaaaaagaa ttccaaagtg tttcattatc gccacaaacg attcaacgtt
     4801 gtgcaccaaa agtaacgact gtaactaatc agacaaataa attgatgcac tcgatcaaag
     4861 aagctataga aaaatcgggc ttaaaagatg gaatgacgat ttcatttcat catcatttga
     4921 gagaaggtga ttttgttttt aaccaagtga tggccgtgat ccgtgaaatg ggcatcaaag
     4981 aattgacctt agcaccaagt tctttaacga acactatgaa tgagatggtt attgaatgca
     5041 tcaaggacgg tacagtaact catatcacaa gcagtgggat gcgcggcagc ttaggtgaat
     5101 ttatttctca tggaggaatg gacaatcctg tgattttacg ttctcatggt aatcgtccac
     5161 gtgcaattga aagtggtgaa ttgaaaatcg atgttgcatt tctaggagta ccaacatctg
     5221 atgattatgg aaatgctaat gcgactgttg gaaaatccgt ttttggttca ttagggtatg
     5281 caaaagtaga tgcacaatat gcagataaag tcattcttct aactgatcat ttagttcctt
     5341 accctaatac gcccattagt attccgcaaa cacaagtcga ctatgtcgtg gaagtagaag
     5401 caattggtga tccgaataaa atcggtgctg gtgctacacg gtttactaaa aacccaaaag
     5461 aattaaaaat tgctgaaatg gtcaaagatg tcattgttca ctcaccttat ttcaaagatg
     5521 gcttttcttt tcaaacagga acgggtggtg cagctttagc agtaacgcgt tacttagcta
     5581 gtgaaatgga agaaaaacag atcaaagctt cttttgcatt aggtggcatc actaaaccaa
     5641 cggttgattt attggaaaaa ggtcttgttg aaaaagtgtt agatgtccaa gactttgaca
     5701 aaggagcagc tgaaggaatg gcgcggcttg agaaccaaca ggagatcgat gcttcctttt
     5761 atgctgatcc aagtaataaa ggagcggttg ttaaccaatt agatgtcgct attttatctg
     5821 cattagaaat tgacacaaac ttcaatgtaa atgttatgac tggttcagat ggtgtgctaa
     5881 gaggtgcgat tggtggacat caagatgcag caacggctaa acttactatc atctcagcgc
     5941 cgttagtacg tggccgaata cctacagtag taaatgatgt tactacattg atcacaccag
     6001 ggaaaagtat cgatgtatta gtcacagaag taggggttgc tatcaatccc caacgtaaag
     6061 atctaatagc tatttttgaa agaattccac aaattcctgt ttttacaata gaagaattac
     6121 agcaaaaagc agaaaaaata gtaggcatcc cggaaccttt gcaattcaca gatcggatag
     6181 ttgcctatgt agaataccga gatggttcaa ttttagatgt cgttcgtcag gtgaaagaat
     6241 ttgaagaaga gagatcatga tgtttgatac tggagaaatt caagaaatcg aggatatttt
     6301 gatccataaa gaacaacgtg ctaacttgca aagcaacctg ctttccaagt attcaaatag
     6361 ttcattgatg gtctttacat tgaatattcc tgggccgatc aaaaataatc caatgattga
     6421 gcaggtattt cgtcagggac agcagtcaat tcagcaaatg tttttaaaaa aaaaggtgga
     6481 aatgacgtac acaaaagaaa tcaatttgcc aagtggattg gactggtacg taatagtaac
     6541 agaagcacct ttgaatctca aaaaatggtt ggtcacgtta gaagagcaag acgcattagg
     6601 tagacttttt gatttagatg tcttgttcaa aaaaggagag cagataacaa ttgtcagtcg
     6661 ccaagctctt aatcatgggg aacgtccttg ttttatttgt gggaaatcag caaaggtatg
     6721 tgcacgtaat cgtacacata ctgtgaaaga aatgcaaaag aaaatcgaac aaatagtagc
     6781 cgattcctta aaaggagcat tgaaacatgc atgataatat tcaaaaaata gcacaagaag
     6841 cacaattagc gatgctttat gaagttacat gtatcaataa accaggattg gttgatccag
     6901 tagaccccgg gtctcatcaa gatatggaca tttttacttt tctacaaagt agtgtagttt
     6961 tgactcccta ttttgaagaa tttatccaaa caggattgga ccgacaacat caaccaattg
     7021 aagaaacctt tatggcgatt cgacagattg gtttagaggc tgaaactgcg atgttctcag
     7081 tgacaaatgg tataaataca cataaaggtg ctatcttttc attggggatt tttcttgcta
     7141 tttgtggccg gttaactatt tggaaagttc cttgtaaaaa aagtttcttt caaaaagaaa
     7201 ttcaaaaaat gacgaaaaat ctacttaacg attttgggaa gattgatcaa acaaaaccaa
     7261 aagcatggac ttggggagaa tatctatttg ttaaccatgg attgacaggg atacgagggg
     7321 aagccaaaag aggttatcct tgtgtttttg accgaggatt gccttattat caagcatatc
     7381 aagggtcgca acaagataag atgatagaca ctttactttt tctatcaatg gaagtagaag
     7441 ataccaattt gatcaaacga agtaataata gacatgtttt tgaagagtat caattactta
     7501 ttcgtcctta ttttgaatta gggggtgtaa aaaccataca aggtaaaaaa tatttggatt
     7561 acctaaatca gcaatttaaa aagaaaaatt ggagtattgg tggaagtgca gacttattga
     7621 tccttacaat ctttttagat aaaatcatgg acaaaggatg gttatgttga tactataata
     7681 cattattttg tattagtaag ctaataacta aaaaatggtt caatacaaat attttataaa
     7741 aaatggtagt gcctatcctt tcagattgta gacaaagaaa aatttctttg tctacaattt
     7801 ttttgttaat gacttcagtc agtttcgtgc gcaaagcgtg cgaaacaaga aaaccatgcg
     7861 tttctacacg tggaagaact agcatatcga aaggagtttt ttcatgtcaa atgacgataa
     7921 aagtttagcc catactcgtt ggaattttaa gtatcatatt gtatttacac caaaatatcg
     7981 acgaaaagta atctataggc aattacgaaa agatattgga aaaatactta ggaagttgtg
     8041 caaaataaaa aacgtggaaa ttatagaagc acatgcaacg ccagatcata ttcacatgct
     8101 tgttcaagta ccatcaaaaa taagtatatc cagttttata gttatctgaa agatcgtagt
     8161 gctgtgctaa tccacgagca gcacgtaaat cacaaatata gatacggaaa taaatcattt
     8221 tggtcggagg aatattatgt gagtacagtc gaattgaatc aaaagacaat tgaaaaatac
     8281 atccgtgaac aagaagctga tgatcgcatt tgagatagta taagtaaaag agagtagatg
     8341 ttgcaataac aaaagggtaa aaatggacta cctccagtaa aataccgaaa aatagtttaa
     8401 cttttggaga taagtccatt taagggtcta tagaatgaca atttttttag gggattttac
     8461 tattattact tcaagacccg cttggattcg caaagtgagt gaactcttaa aactacctga
     8521 tagtaggttg ttttaagagt tatctgaaag taaaaattgc atttatattt ttgataaata
     8581 cgtaaattta tatatacgta tttatcaaag ttttaggcca aaggaatatt gtgtaaatac
     8641 actaggattg aatgaataga ccagaagaaa ttgcgtccaa gaacaaaaga agcatgatag
     8701 tgctctatat aaattgggca ttcaagaatc tgaaatttaa aggtagaatt ttagttagac
     8761 ttttagattt taaacacaaa aataaccata caagcaacaa gaagcaaagt gctatattat
     8821 aaattgtccg tccgtttact tacataagat cattggggct atttttaaaa gagaataata
     8881 gataataaat gaaaaaaaag tcaagtccac aatcctgtgt aaaatactat acaatgctct
     8941 cactattttt tacttggcaa atttaatata ttttcttgat gaagaaagcc actcatcgtg
     9001 ttgatcaata aggactgctc caattaaacg attggctgaa acgcagtttg gaaagatacg
     9061 aataattttt tctcttctgc g
//
LOCUS       DACEMS010000104         9055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEMS010000104 DACEMS010000000
VERSION     DACEMS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.107"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            806..1006
                     /locus_tag="IW735_002187"
                     /pseudo
     CDS             806..1006
                     /locus_tag="IW735_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="IW735_002188"
     CDS             1493..1672
                     /locus_tag="IW735_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045172.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="IW735_002189"
     CDS             complement(1718..2323)
                     /locus_tag="IW735_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1045173.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="IW735_002190"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="IW735_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3005..3232
                     /locus_tag="IW735_002191"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="IW735_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="IW735_002192"
     CDS             3358..4329
                     /locus_tag="IW735_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1045174.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="IW735_002193"
     CDS             4469..5782
                     /locus_tag="IW735_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1045175.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="IW735_002194"
     CDS             5825..6691
                     /locus_tag="IW735_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1045176.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="IW735_002195"
     CDS             6688..7536
                     /locus_tag="IW735_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1045177.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="IW735_002196"
     CDS             7550..9022
                     /locus_tag="IW735_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1045178.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
BASE COUNT         2885 a         1506 c         1843 g         2821 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg taaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaaatttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaatgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgtgttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggaaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttttaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaat
//
LOCUS       DACEMS010000105         9009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEMS010000105 DACEMS010000000
VERSION     DACEMS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.108"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(50..1855)
                     /gene="glmS"
                     /locus_tag="IW735_002197"
     CDS             complement(50..1855)
                     /gene="glmS"
                     /locus_tag="IW735_002197"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1045179.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2264..2554
                     /locus_tag="IW735_002198"
     CDS             2264..2554
                     /locus_tag="IW735_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045180.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2567..3592
                     /gene="sppA"
                     /locus_tag="IW735_002199"
     CDS             2567..3592
                     /gene="sppA"
                     /locus_tag="IW735_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1045181.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3595..4254
                     /locus_tag="IW735_002200"
     CDS             3595..4254
                     /locus_tag="IW735_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1045182.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4411..5343
                     /locus_tag="IW735_002201"
     CDS             4411..5343
                     /locus_tag="IW735_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045183.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5497..6786
                     /locus_tag="IW735_002202"
     CDS             5497..6786
                     /locus_tag="IW735_002202"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1045184.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            7033..8283
                     /gene="clpX"
                     /locus_tag="IW735_002203"
     CDS             7033..8283
                     /gene="clpX"
                     /locus_tag="IW735_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1045185.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8366..8953
                     /locus_tag="IW735_002204"
     CDS             8366..8953
                     /locus_tag="IW735_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1045186.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         2993 a         1574 c         1911 g         2531 t
ORIGIN      
        1 aagaacaagg accagtgtca gatgtctgac actggtcctt gttcttttat tattctactg
       61 tcactgattt cgctaaatta cgtggtttgt caacgtcgaa tccccgtaat agcgttgcgt
      121 aatatgcaag taattgagtc ggtacgactg tggttagagt agttagataa gggtggacag
      181 ttggtagcac cagctggtca ctttcctttt ctaatccctc tgagacaata actagtgttt
      241 tagcgccacg gctttcgact tctttcaggt ttcctcgagt atgcgcacca gtcacttctt
      301 cactgatgat tccgatcact ggtgttcctt cttcgatcaa agcaatcgtt ccatgtttca
      361 attcgccagc ggcaaaacct tctgcctgga tataactgat ttctttcaat ttcaaagcag
      421 cttcaagaga aacgttgtag tcagcaccac gtccaatgta aaaggcattt cttgtatcgc
      481 tgagatatac ttctgccaac tcttctaaat aatctttttg ggcgatgacc gattccatcg
      541 cattagcaac taaactcaat tcatgagtga tatcgaatac aagagaagtt tcaaattctt
      601 ttttatcacc gatagctttg gctaataaag ttagtacagc tatttgtgca gtataagctt
      661 tcgttgatgc tactgcgatt tcgggtccag catgcaatag cagtgtaaag gatgcttccc
      721 gcgaaagagt agaaccagca acatttgtaa tcgtaagtga agggtagccg agacggttga
      781 tttttaccag cacttggcgg ctgtctgctg tttcgccact ttgactcaag aaaataaaga
      841 atggtttttt tgaaagaaga ggtgtgttat agccaaattc actggctaaa tgaacctcaa
      901 caggaatctg tgtcaatttt tccaaagtct gtttacctgc taaccccgca tggtaactag
      961 ttccgcaagc aacgatatag attcgatcgc tatcagacaa tgtatcgacc agcttttgat
     1021 caagagagac acgattgtct tctgttacgt atttttgagc gatacgacgc atgataatag
     1081 gctgttcgtc gatttctttc agcatataaa aaggataggt acctttctcg atatctgaag
     1141 catctacttg tgcttcgaat gaagaacgtt ctacgatgtc acctgcaaaa ttttgaataa
     1201 taactttttc tgatgtgacg gtaaccatct cttcatcttc aatctcaacg aattccttcg
     1261 tatgagcaag catagccatt gcatcgctgg caatcacatt gaatccatcg ccgagtccga
     1321 ttaatagtgg acttttattt ttagcgacgt aaataacatc aggtgctgtc cgatcaatca
     1381 aagcgaaagc gtaagaacct ttgatgaccc gcaaagcttt taaaaacgcc tcttgcgttg
     1441 tagtatcctt cgcgaaagtt tcaattagat gagcgataat ctcagtatcg gtctcaccaa
     1501 taaagtggtc atttgctaaa taagcaattt taagttcttc aaagttttcg attacgccgt
     1561 tgtgaacgag cacgaagcgt ccactttggg aagtatgtgg atgggcgttt tcttcagaag
     1621 gttgcccgtg agttgcccat cgtgtatgac caatacctat atttcctgta gtatcaatat
     1681 ttattttgtt tttcaaattt tgaattctgc cttgtgcttt gaccagataa tcagttgtgc
     1741 catctgagac aaagatacca gctgaatcat aaccacggta ttctaatttt tctagaccgt
     1801 caatcaatcc tggtgttaca ttttttaatc caatcattcc aacaattccg cacataaaaa
     1861 acatcctact ttctataatg aattcgtatg aatgcttcca gctgcaggaa aatccttttg
     1921 ttttatcttt gcagacattt tttgtggatt ttctgtagag tgcagtccct agggccatcc
     1981 gccgaatctt tcgataaacc ctctcctcgt catctgttta cacagacctg gcgctatcct
     2041 aaaaggtata actatcctcc tttttgtttt ttatctggaa acatttaaaa tcataacctt
     2101 ttgcgaatgt tcttgtcaac aaaaaaagaa taaaaaataa attggtatat atcaaattct
     2161 ttgtcaaatg tcctttttat gtcaaaaaat aatgggtttt tatctatcat ctttttcagg
     2221 agttcggtac aatagaaagt aatcggatag aggaggagta aaaatggatt caaaaattga
     2281 gatcatgaca ctgggaatgt taaaaaaaca attgtccaaa ttcgaagcat cagctggtgt
     2341 atcagatgat acgaaaatat ttttagatac tggctgggat agcatccaag aaatcgcacc
     2401 tgatgccttg gaagttgctc aagctcgtga gtttacggta gaagatgaat taacaaaaga
     2461 gtcgttttcc gggtatgcgc gggaagaaaa agcagagcgg ttcgacactt ctgagcagtc
     2521 agaaactgtg atcgtcataa aaaacttgta ttagaaagga gaaagaatga ataaaagacg
     2581 ttgggcagca gttctgattg ctgcaggatt atttgtggtt tcgttagttt cagctagttt
     2641 gactaagcct caggaaaacg atcaaattga gggattgaac agttggttat atggggatga
     2701 agaactagct ccaaatgtgt tggaagaggg agacagtacg caaaaaatcg taaaattatc
     2761 agtagatgga acgattgctg cgggagggtc ttcaggttta tttgcttctg aaggttacaa
     2821 tcataaaaat tttatggaac aactgaaggc gattgaagaa gatccaacag tcaaaggtat
     2881 ctttttagaa gtaaattctc ctggtggcgg tgtctacgaa agtgcggaaa tcgctaagaa
     2941 attggatacg attcgtaaag aacatgatat cccaatgtat gtcagcatga aaaacatggc
     3001 agctagcggt ggctactata tttctgctca agcagataaa atctttgcaa cagaagaaac
     3061 agtgaccggc tcaattggtg tgatcatgtc aggattgaat tattcaggac ttctagaaaa
     3121 actgggtgtg gaagatacaa cagttaaaag cggagcgttg aaagatatgg gttcaggaac
     3181 gcgaccagag acaaaagaag aagaagcggt gcttcaagca tatatcgaca gcgcatatag
     3241 tcgttttgta gcaatcgtca gcgagggacg gaataaatct gaagaagaag tcaaaaaaat
     3301 tgctgatgga aggatttatg atggtgtcca agcaaaagaa gtaggtttag tcgatgaatt
     3361 gggatttcct gaagatgctt taaaagctat acgaaaggaa caacagcttg aagatgcgga
     3421 actagtagaa tattcttcca cgccatctgg gtttgctagt acatggttag gaagtaaact
     3481 agcagaattc cagggattga agccttctga gactagtcag atcttatccg ttcttgagaa
     3541 tctagggaca gcggaatcac caaaagcaat gtattattac ggaggtgagt aagcatgtca
     3601 gagctttcaa atgaaaataa ggaacaagaa aaagataagc aacagcagcc tgctgtagga
     3661 cctgactcca acttttccca aaaggtactg agctcttttg aaagagagcc tttgacgccc
     3721 gaagaaatca aaaagaaaca acgtaaatgg aaaaagtata cggacacaag aaatgaaccg
     3781 ccaaaagcaa atgattttcc taagtatttc tatgcaggtt tttttatccg aatgtttgcc
     3841 ttcttggtcg atcttttgtg tatcggagct attacgcgtt taacacttgg tattgccaaa
     3901 aatctaggtt ggcttaattt ttcagattct tatctgagtc tctacggatt cacagcctta
     3961 cttatttatc tagcgtattt tattttattg acgaaattca atcatggcca aacgattggg
     4021 aaaatgatct ttggtattcg agtcatcagc tttgatgaag aagaacttag ctggtcaacc
     4081 gttttagtta gagaaggtgc ttgccggttc attctaaaat ttcctttgct gatgttaggg
     4141 tatcttccag cagtctttaa tcagaaaaag caacatctgg gcgatttttt cactaataca
     4201 agtgttgtta cgatcaatct gatcaaagcg ttcaaccagg aagtcaacag ctgatgagca
     4261 ataaaaagca gtaacagttg aaagcacctt ctaaaagtca gatctaaaat ctagctttta
     4321 gagggtgctt cagttatttt taattgtcat gctttttttg ttttgctact attgaacatg
     4381 agctgaatat gagcaagagg aggacactgc gtgaagccga ttgattttcc agaaaaatat
     4441 gagaatttaa tgcgagtggc acaacaagca ctagcaaacc agcaatatca gcaggcaaaa
     4501 gaattatttc aacgagctta cgagttgaaa gaaagctttg aggctaatag cctgcttgtt
     4561 ttttgtcttt acgagttgga cgagaaaaaa gaagcattga agcaagcact cttgcatgaa
     4621 aaacagtatc ttcaaaatga ggagtttgct gaattttatt ttgacttgtt aatcagtaca
     4681 caagattttt tatatgctag aaaattgata gcaagtacag atttttatga agctttcgag
     4741 cagcgggtca ttgaaaaaat ccaatttgca gaagaattat ccggacaaat ggagaggcag
     4801 aagatcaagg cactccacaa taaatcggag gaacttcctt cgttggaacc tgcacgtcag
     4861 cttgcgtcta ttgatgagat cgaacagctt ccttatcatg agtttatcca aacagcgagt
     4921 aaacttattg tatcgccaga ggtccatatt ttagcaaggg caaaattact ggagacactt
     4981 aggcaattga acgaatgcca tcctgttttc tatttgacga ttgaagaaaa gttagtcaaa
     5041 gtcataccaa aagatctgcc aaagccgcag cagcaggcaa gttaccagca attatgtgta
     5101 ttttcagatc gttacgggaa tgaagatgcg ctgcttagtt ccgtgttaaa agaagaattc
     5161 acgttgcaat ctgcaattgt ttatcctgtc tatgatacat acatcgaaga cccgaagcgc
     5221 tggtttcaac tgactgtaga ggcgtgtaca ggaaaaacga tgtatgaagg tacagaagat
     5281 gaaaaacaag attttttaaa aaaaagagaa aaaattttgc agcaaatgat cttcttccat
     5341 tagattttat cagtgaaaaa gataaaaatg ttatgaaaaa caaggattct atccattcaa
     5401 tatgtttaca atttgaaagg gttagtgtac tatttaaagg tatgcatgaa acgtttttga
     5461 tgtataaaat gaatttctaa actcggaggg aattaaatga ctgcaacatt tgaaaaaaaa
     5521 ggcacgaatg atggtgtttt aaaatttacg attgcgcaag acgaaatcca aaaaggatta
     5581 acaactgcgt ttaataaagt gaaaaaaagc ttgaacgtac ctggattccg taaagggaaa
     5641 gtttctcgtc aagtattcaa ccgtatgtac ggtgaagaag ctttatatga agatgcattg
     5701 aatgctgtat taccagaagc ttatgaagct gctgtcaaag aagctggcat cgatcctgtt
     5761 tctcaaccaa aaatcgatgt tgaaagcatg gaaaaaggtc aagactgggt aatcactgct
     5821 gaagtaacag tgaaaccaga agtaaaatta ggacaatata aagatctagt tgttgaaaaa
     5881 caagaccgtg aagtaacaga tgctgacgtg gatgcacgta tcaaacgtga acaagaagca
     5941 caagctgaat tagttatcaa agaagatgct gcagctgaaa atggcgatac agtagtaatc
     6001 gacttcgaag gctttgtaga tggcgaagcg tttgatggcg gaaaaggcga aaattactca
     6061 cttgaattag gttcaggctc attcattcca ggttttgaag atcaattagt tggtcataaa
     6121 tcaggtgaaa gtctagatgt aaatgtaact ttcccagaag attatcaagc agaagatctt
     6181 gctggaaaag aagcagtctt caaagtgaca atccatgaag taaaagcaaa agaagtgcct
     6241 gaattagatg acgaattcgc aaaagatgtt gatgacgaag tatcaacatt agacgaattg
     6301 aaagaaaaat atcgcaaaga attagtagaa acaaaagaaa aagcagcgga cgatgcaaaa
     6361 gatgaagcag cgatccgtat ggctgttgaa aacgcagaaa tcgttgaatt gccacacgtg
     6421 atggttcatg atgaagtaca tcgttcaatg gacgaattct tgaacaacat gcaacgtcaa
     6481 ggtatctctc ctgaaatgta ctatcaatta actggttcta cagaagaaga cttgcataaa
     6541 caattcgaag gtgaagcaga aatgcgcaca agaacaaatc ttgtgatcga agcaattgca
     6601 gctgctgaaa atcttcaagc aacagaagaa gaaatcaatg cagaaatcca agaattatca
     6661 gaaacataca acatgccaat cgaacaaatc aaacgcgtgt tgacagaaga tatgctggaa
     6721 catgatgtga caatgaaaaa agcaatcgaa ttgatcacag gtacagcgac agaagcgaaa
     6781 gaatagaaat caagtaataa atcatttctg aagaagctac ttctagaaaa ttgtttattc
     6841 gaaataaggg actgtgacac gattttgtca cagttcctta ttttttgtta agagtaaaaa
     6901 atgcaaaggt ataggtcagt tgatcctttg ttaaacactt tttcttttta ctaaactatg
     6961 ttatgatagt aaaagactgt aaagtctttg atagtttctt gcatactttt tgcaagttaa
     7021 ggggtgaaga ccatgtatga caatccaagt agcaatgaga ccgtacgctg ttctttttgc
     7081 gggaaaaccc aagaagaagt gaaaaagatc gtagctggtc caggggtcta catctgtaat
     7141 gaatgtatcg acctatgtaa agaaatcatt gatgaagaat tttatgaaga agctgtacgc
     7201 gaattcactg aagtccctaa accactagaa attcttgaag tattgaataa ctacgtgatt
     7261 ggacaagatc gcgcaaaacg ctcgttagct gttgccgtat acaatcacta taaacgtgtc
     7321 aatcagcaag cacaagaaaa cacagatgat gttgaactgc aaaaaagcaa tatctgcttg
     7381 atcggaccta cagggtctgg taaaacattt ttagcacaaa cattagcaaa aacattgaat
     7441 gtaccttttg caatcgcaga tgcgacaagt ttgacagaag ctggttatgt gggggaagat
     7501 gtagaaaaca ttttgcttaa attgcttcaa tctgctgatt acaatgtgga acttgcagaa
     7561 aaagggatca tctatattga tgagatcgat aaaattgcgc gtaaaagcga aaatgtatct
     7621 attactcgtg acgtttccgg cgaaggtgtc caacaagcct tgctgaaaat tctagaagga
     7681 actgttgcaa gtgtgccacc tcagggcgga cgtaaacatc cacatcaaga atttattcag
     7741 attgatacga cgaacatatt gttcatcgta ggtggtgcat ttgatggcat cgaaacgatt
     7801 gtgaagaatc gtatgggcga aaagacgatt ggatttggta cacaaaacca aaaactttct
     7861 gaagacgaaa gtgtcatgca gcatatcatt cctgaagatt tactaaaatt cggattgatc
     7921 cctgagttta tcggacgatt acctgttatg gcagctttag agaaattgac aacagatgat
     7981 ctggtacgga tcttgactga gccgaaaaat gcgctagtca aacaatatca aaaacttctt
     8041 tctctagata ataccgaact tgaattcgaa cctgaagcgt tgagagccat tgcgaaaaaa
     8101 gcaatcgaac gcaataccgg tgctcgagga cttcgttcca tcattgaaga aatcatgatg
     8161 gatgtgatgt ttgatatccc ttcaaatgaa aacatcgaaa aagtgattat cacaaaagaa
     8221 gcagctgaat tgtctggtga gccaacagta atctataaag attcagagaa aaaagcgggt
     8281 tgatgaaaag aggctgccgt aaaagtcatt ttggcttttg cgacagccgc ttgctttatt
     8341 catcaccttg ttaaggagag agaccatgaa agtccatcaa gctgaaatta tcataagtgc
     8401 agtggcacca aaacaatatc cagatacaca attgccagaa atagctctgg caggacgatc
     8461 aaatgtaggg aaatcttctt ttatcaacac attgattgat cgaaagaatt tagcccgtac
     8521 ttccggtaag ccaggaaaga cccagacatt gaatttttat ttgatcgaga acgcattaca
     8581 ttttgtcgat gttccgggat atgggtatgc aaaagtatcc aaaactgagc gagcaaaatg
     8641 gggaaagatg atcgagacat atttaacgac tagagaccag ctacgtgcgg tcgtttcttt
     8701 agtagatttg cgtcataagc cttctcaaga agatatccag atgtatcaat ttttgaagta
     8761 ttatgatatc cctgtaattg ttgttgccac taaggcagat aagatccctc gtggtaaatg
     8821 gaacaagcat gagagcgaaa tcaaaaaagc gttggatttt gatcgcaatg acacgtttat
     8881 tctcttttct tccgttacaa aacagggaaa agaagaagcg tgggcagcaa tcgaacaagc
     8941 aattgaatca taaaaagaat aaaaaagact taccagtcaa actctttcat aaaagtttgg
     9001 ctggtaagt
//
LOCUS       DACEMS010000106         8569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEMS010000106 DACEMS010000000
VERSION     DACEMS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.109"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(305..835)
                     /locus_tag="IW735_002205"
     CDS             complement(305..835)
                     /locus_tag="IW735_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1045187.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1017..1673)
                     /locus_tag="IW735_002206"
     CDS             complement(1017..1673)
                     /locus_tag="IW735_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1045188.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1800..2273)
                     /gene="dfrF"
                     /locus_tag="IW735_002207"
     CDS             complement(1800..2273)
                     /gene="dfrF"
                     /locus_tag="IW735_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1045189.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2692..2910)
                     /locus_tag="IW735_002208"
                     /pseudo
     CDS             complement(2692..2910)
                     /locus_tag="IW735_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2941..4155)
                     /locus_tag="IW735_002209"
     CDS             complement(2941..4155)
                     /locus_tag="IW735_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1045190.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4496..4738)
                     /locus_tag="IW735_002210"
     CDS             complement(4496..4738)
                     /locus_tag="IW735_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1045191.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4770..5327)
                     /locus_tag="IW735_002211"
     CDS             complement(4770..5327)
                     /locus_tag="IW735_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1045192.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5340..5528)
                     /locus_tag="IW735_002212"
     CDS             complement(5340..5528)
                     /locus_tag="IW735_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1045193.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5541..6107)
                     /gene="amaP"
                     /locus_tag="IW735_002213"
     CDS             complement(5541..6107)
                     /gene="amaP"
                     /locus_tag="IW735_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1045194.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            7337..7810
                     /locus_tag="IW735_002214"
     CDS             7337..7810
                     /locus_tag="IW735_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1045195.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7819..8046
                     /locus_tag="IW735_002215"
     CDS             7819..8046
                     /locus_tag="IW735_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1045196.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2607 a         1684 c         1273 g         3005 t
ORIGIN      
        1 aaatcaactg tctttctcat atttactatt caattttcat agtgatatag gtacaattta
       61 tatcactact gtaaaaatgg ggttttgcct tataaataca gcgttttccg cttgttagtg
      121 tatagtgttt cttgtttttt gtgcccccct tgttagataa cgggggcttt gaacggctcg
      181 caagctcgcc gaaccgccga agcgaaccgc ccccttgcaa gcatggcgga aaaatcttaa
      241 ggattttccc gccaacgtgg tcggatcgct ccgtcggttt aacagctttt ctgtttctct
      301 ctagttatct acgcatataa atcaaatcgg ttattccgtt ataagtctgt gttttacaaa
      361 gccttaactt tatttccctg tctatgttgc ccaaaagacg aaccccattt cctaacagag
      421 taggaataac agaaatgtaa tatttatcaa tcatattttt gcttaccaac tgctgaacaa
      481 gatttgctcc gccacaaatc caaatatcct ttcctgcttc ttgttttagc cgttccaata
      541 ataaaacagg attttcactt gtaaaatgaa tttgttggga agaattgcac tctttatgtg
      601 ttaatacata agttgtaaat ttattatata cccattcttg cggagaaagt tcagtaacaa
      661 cctgatgaga agtattccac cccattaaga cagtatctat atctttggta aattctgtat
      721 aagtgtcgat gttttcattg tcatttccat gaccatttag ccaagctact ccaccattac
      781 cgtccgcaat atatccgtca aggctcatgg caataaataa aactactttt ctcatttttt
      841 ctcttttata aatacaaaca ggaatttatt tctctttttt actcataagg gaacaactga
      901 ttgctacaaa taaacccatt aaaatccatg gaaatgctgc tcttataaaa tcactaacca
      961 tactttattc ctccttaaat tccaatttcc ttagttctac aaactgaaat ttttgcctat
     1021 gaatgtatct tgttctttat acgcaaacag gcttctgtgt gcataatcca atgtcttgaa
     1081 aacggcattt gaattagtcc acaaatatct ttattacacc aataatcaat cagccaaatt
     1141 gctttttcat tgtcgtttac tacattcaac gattctaagt atccttttct ttctttcggt
     1201 atttttcttt tcaattcatc ataagataac cgttcaagca ttttctcagt gctttcccat
     1261 acttcgaaaa tatataaata taattcttcc agattaagct gttttgaaaa gtctgcaatc
     1321 tgctgtttca tgagttcatt tcctgttgta atgataggtg aattgatacg ctcttgataa
     1381 ttgcctgcaa aaaatacttg ttcatcttca tttatcactg tatgcacaac tatatcttca
     1441 atgcggaaaa tatgccaaat agaataagca atcgtcttac tatgataacc atcagcgttt
     1501 atgaagggga ttgagttaaa atcttctctg cataattctt ctttgaaaga tactaaggtt
     1561 tgtattaact gacttcgtaa agtaagtaaa gtatcaatac ccctcttata agtatctttc
     1621 ttttttattt gtgcctgcat tgttttatta agttcagacc attccttatt catattcaat
     1681 cctctctcaa attctgattt tatcgcccca tacaaactgg aatttgtctt acaacaattt
     1741 atatatgcat aaaaagcaat atatttatga aaacacccta aatccaaaat ttactcaatt
     1801 cattttttcc ttacataaaa tgttctcgta tatttcactt cctcaccaag tgtttcccct
     1861 atcaaaactt caaaatcatt gatatcaaat tctggaaaga atgtatctcc atcttcaata
     1921 tttaaatcta cttctgtgat atacatttta tctactattt gcaaagcttc cttaaatagt
     1981 ccatatccac cagatatgta tacatctcgt cctttagcca ataataatgc atcttctaat
     2041 gatttaactg aaactaaatt atctccttga tactctgttg tggtggaaac aacaatattc
     2101 attctattag gcaacggatg accgatttct tcataagact ttcgccccat aataaccaca
     2161 ttacccgttg ttaactctct aaattgcttt tgttctccct ttattttcca tggtatatta
     2221 ccattcttgc ctataacatt attctttgac ctcgcaacaa tcaaacctat catctatttc
     2281 atcctccata atatgatgtc gtacctgtga ttccatcaag tgcaccacat cttaacaaac
     2341 tcaaaattat ctcgaactaa ccagctcgac aaattccttt tttgtcttca tctgcattat
     2401 atcatattgt tataagcatt gccatttctt ttctgctttt gaaaagttac ttatcagcct
     2461 atgttttgta aagggtgata ttcagcattt ctcacgcaac aagcccttga caaaccatag
     2521 actaataagt tttaaggtaa tcaagcagaa atgaaatagt ctgctgtttc acggcaacaa
     2581 aaaagagata ccaacacact tatgtagtat ctcttagtct agtagcaata ttcttttgtt
     2641 tgcaagcagg taaacatggg ctgtttctta atatcttttt gtttgggaaa cacctttttc
     2701 ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa accatagcaa
     2761 accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg tgggatattc
     2821 atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaacg ttaatttttt
     2881 tgcaggaatt ttcgttttgc tttgttgcat gaatcttcct cttttctatt tgaatttgtt
     2941 tcaattgagt ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt cttcttttgt
     3001 agtagtgatc gttgtaaagt gcatcgccga ttttaaaatc actgttgtca gcacataggc
     3061 atacacggca gtaatgatga caccaatcac ttgaatccct aagaagtacc agctgcttgg
     3121 attcgttagt aaatggctgt tagatgcaaa gatccctatc agaatcgtac ctgtgaatcc
     3181 gcccatgcca tgaacacccc acacatcaag tgcatcatcc cattgtttct ttttacgaaa
     3241 atcaacagca gcatgacaga cgatacccgc aatgatccca atcagcatgg cactttttgc
     3301 ttctacataa ccggcacaag gcgtaatggc tgccaatcca gcaacggatc ctgttagtac
     3361 gtcgacaaaa tcaaagccat ttccttttac ttttgcaatg atcagccaag taatcatcgc
     3421 aaatgcaaga gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat ctgctcgaag
     3481 tgcaccaccg gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac cgatagcagc
     3541 tgccataaga ctgctatgct gacttctttc tcctaaagga atctttcttt gaccaagtgt
     3601 gaggatacat gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc caccagcgaa
     3661 atctctaaat cccatctgat ccaaaaatcc gccaccccat atccagtggc aaacagggaa
     3721 ataaatcagc agattccaaa aaataacaag tataatatag ccttttaaat tcaatctccc
     3781 tgcaaaagct cctgtcatca aaggaacggt aatcacgata aacatcagtt ggtaaagaaa
     3841 gaacattaaa aatggaatcg ttgcaccatg aaacatgttg gggaagcttg taacatgtct
     3901 catcaaaaag aaatctgccg ggtttccaat aacaccgccg atatcccgtc caaaggccaa
     3961 tccgaatcca ccaaataccc acataatcgt tgttatccca acggagagaa aagattgaga
     4021 catgatcgtt agtatattat tcttccgtgc taatccgccg taaaaaaaag ctagaccagg
     4081 agtcatcagg caaaccattg ctgtacataa aatcatgaat gctgtatctc ctgtattgat
     4141 aggtaatgta ttcatttttt tcgcctcctt tctacgatta cgagtataaa agaaggtgta
     4201 gttgtttttt tcttctgttt ttttgaatta atacttgcat ttataaaaga cagacaatta
     4261 ataaaagctt gatgtgttgt gctctttttc gtttttcaca caaataaaga caaaaaataa
     4321 acggtcttct ttttatagaa aaccgtttat tttcgtcttt ttttattttt aaagatattt
     4381 tttcataagg aataaagaat acttcatata aaatatataa aaaagaaccg cagcgagtgt
     4441 cgctacggtt ctcaaatttg ttcttcattt caacataagt ttttcttcaa tttgcttata
     4501 atttacgtgc aaagaatgaa acgactaata ctaaaatgat tgcaccgatc aatgatggaa
     4561 tgatagccat tcctgcaatt gttggtcccc aagttcctag taaagcttgt ccaattgctg
     4621 aacctactaa accagcaatg atgtttgcga tgatccccat tgatgatcct ttattcgtga
     4681 ttgctcctgc aattgctcca atgataccgc cgacgattaa tgcccaaata aatcccattt
     4741 taaattcctc ctaactgact tcttacttct tattgtacac gtggttcgtt attttctttg
     4801 actttttgtg cacctttgcc tacagcagat tttgctttat ctgtttggcg tgaagcaaat
     4861 tgtcctgtgg attttgctgc ttctgttact ttgtcttgta ctgtttcttg atctttttgg
     4921 tattcttctt gtgttttgat gtctgcaaca ttcacattca cttcgataac atctaaatgt
     4981 gtcatattgt ttacttcagt actgatcaat tctttgattt ggttgtaaat atcttcgatg
     5041 tctttaccgt attctacgat cacatctaga tccacagcta cttgtttctt acctacttct
     5101 gtattgatac ctgctgttac gttatctgtg ttgactaatt tttcagccat atttgagaaa
     5161 aatccgccat ctaatgtcaa tagaccatct acatctttca atgcataacc gatgattttt
     5221 tggatgactt tgtcttcata agtcaattca ccattcatca tgcggttttc tcctttttca
     5281 tttgccaaaa ctgatccatt tgttgtattc atgtttttgt tttccatctt tgtttcctcc
     5341 tatgatttaa aaaattgatc aactattcct gactgtttca aataaaaacc gattcctgcg
     5401 ccaattgctg tcatgattat gatcaacaat gtcttgaaaa atccaatcgt taaccataaa
     5461 acagctagca gtagtccaag acccccaaag ataataggga gcttgtatgc tgaaaatagt
     5521 tctttcatta tttggccctc ctattccact cgagagtgct tgtagtctct tgtatcttct
     5581 tgttcttcat aaccgctgta tgtgactgcc actttgaccg tttcttttgt tccgagtact
     5641 ttagttactt cttcttcgat atcttgcatc aatacacctg ttttccctac taatgaagaa
     5701 gttcgtttca agtctccgtt gacatggata tggattcgat ttttcgttga acgtatccgt
     5761 actttaggtg aatgaatgaa ctcttcttcg tgtaaatggg aacgtaccaa cccttcgatc
     5821 gcttttttgt cgattgttag tttgccatct tcccgtttca taacgaaagt tcctcttgac
     5881 tttggataga aaagaacgac tagcaagatg ataatagcaa gaacggcaaa tactacagct
     5941 gcccagaaaa tatattgttg catgtagtaa ttcgttaaag taaagaagcg aaatcgttcc
     6001 aattgttcag gcataatata cggcgcattg atcaacgcaa tcgctagaaa tactgaaagt
     6061 agcagtaaac tcatgataac accgattatt tttaacccct tattcatttt ccacctccta
     6121 taaacttatt tttaccctgt aaataactct accgacaatc acagtataga tagcgaacag
     6181 ttagcatagc atcagtaaat aaagcgctac caaacattgc tggatcataa cggcgtttta
     6241 ttgccagtct gatactgaaa taacaattgc ttacttattc tctatttact tttgattgct
     6301 ggctatactt aaaggatatt attcaagatt aaaaattgca aagattatgc tcagttatta
     6361 ttacaagatt ctatttattt attaaatttt ttcggagaat taaaaaatac aataaataac
     6421 tttagttact ttaatgcgtt cttactttat ttttatatta tcatttttct tattcacttc
     6481 tttggtttat actattctta taaataaaaa cgaggtatgt cctatgatga aaagattgta
     6541 ctattcattg atcataacga ttggctattt gattgtatca aatctaggaa atatggtatt
     6601 tggtatcagt aaagaattca gttggactac tacattatgg gaatctctat tctttttcat
     6661 cttcgtattt ttgttacaaa attatcgaaa aaaataaaca tgtgccatct atctgttggc
     6721 acatgtttta ggatttatgg tactttttat tcggcaatcc attttatttt tctacttctt
     6781 cttaatacct ctaacacttt tgatttataa actaatccaa tgcatctact tgcgcttatt
     6841 aagctatttc agctagatag taccacccca tgcgtcaatg cttgattttc cttttgtata
     6901 acattgtata agcaataaag gacaaataag tggtgattac accaaaccat tgaataagaa
     6961 aaattctgat acgtaaataa acatgaactt gaaaaaatta atgaatcggt tagctactac
     7021 gtagttgtct tttttctcat acagttctgt taacttacac ttctcttcgc tagataataa
     7081 aaaatcctcc aactgtattg tccaatataa tcccaggcat ctaaactagc attatagaag
     7141 agacacacat agaatctaaa ataactggtt ttaaatgttt cttacaatca aaaaaagctg
     7201 ttaaatgcac tccacacctt catactctat cattttttat aaacgatata tttttattgt
     7261 ttttcgataa aaaatagttg ttttacaata attattctgc tatattttag ctatcaaaga
     7321 taaaagaggg ataaaaatga aatttaaaac attgctatta aaatttgtcg ttttaggtat
     7381 aagtgccgta tttattttgt tcggcgtact tttttatact cagatcgcta caagcgaaaa
     7441 aggattcaat tttgattggc agaccacatt atttgtttct gtcatttact ttgcccttct
     7501 cctagggatc gttattgctt atttcttatt gaaactattg aaaaaaatcg aagataacca
     7561 agctttttcc actcaaagct taaacatact aaaaaaaatc gaacgaacca ttttgtttat
     7621 tggtttcgcc ttttttggct tacttcctaa atttatgaaa gtagcggatt tagacgatgc
     7681 tccaggtttg atgttggtag gaatcgctgt cgtattcatt ccttttgcag tatacacatt
     7741 aacaagcgtg ctggaaaaac tgcttgaaca tgcgatttta cttaagacag atcatgattt
     7801 gacagtgtga ggtgcgacat ggcaattatc gttaatttag atgtagtatt agctcaaaaa
     7861 aaaatgagtg tgactgaatt gagtgagaaa gtgggcatta cgatggcaaa catctctatt
     7921 ctaaaaaatg gaaaagcaaa agccatccgt ttttccactc tcgaaaaaat ctgccaagtt
     7981 ttggagtgtc aaccaggtga tttgttgacc tataaaaatg actcgaatac tataccagaa
     8041 gaataaaata aagtagaaca aacagtaaat aaaaaataat ctgaatgata gatgtgtgag
     8101 acttttagaa gttatttacg tctattattc agatttttta tttcgattga ataccgtttt
     8161 atctataaat aagatatgta ctgactcata caaagggggc cttttcttct ccatgtacta
     8221 tcacttttcc tgatacaact ggtttcctgt tacgttgtag ttggatctgc ttttagtata
     8281 atactttatt cattccacca gactgatagc agtttgagca gtctaaataa tcatgtagta
     8341 ttacaggcca ttctgtttca taccttgaag tgcttaaaac ttcagctaat ggcccattgt
     8401 gttaactctt actccattct tttttagtaa gctcccatat atactgtaaa gccttctata
     8461 tattatgtca tgttgctttt taacatttac tatgtagttt tgataaaatg atttgaataa
     8521 cagtgatgga caattctttt ttatttttaa cattattttg ataataatg
//
LOCUS       DACEMS010000107         8421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEMS010000107 DACEMS010000000
VERSION     DACEMS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.110"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            467..2104
                     /locus_tag="IW735_002216"
     CDS             467..2104
                     /locus_tag="IW735_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1045197.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2249..2989)
                     /gene="truA"
                     /locus_tag="IW735_002217"
     CDS             complement(2249..2989)
                     /gene="truA"
                     /locus_tag="IW735_002217"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1045198.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3180..3815)
                     /locus_tag="IW735_002218"
     CDS             complement(3180..3815)
                     /locus_tag="IW735_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1045199.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3962..5203)
                     /gene="sstT"
                     /locus_tag="IW735_002219"
     CDS             complement(3962..5203)
                     /gene="sstT"
                     /locus_tag="IW735_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1045200.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5401..6397
                     /locus_tag="IW735_002220"
                     /pseudo
     CDS             5401..6397
                     /locus_tag="IW735_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6413..>8421
                     /locus_tag="IW735_002221"
     CDS             6413..>8421
                     /locus_tag="IW735_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1045201.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2783 a         1546 c         1293 g         2799 t
ORIGIN      
        1 aatcagaatc gtacaaatat ttaataacca aatatataga acataactta atattaagtt
       61 atttgaaatt ccataaaaaa cgactgcagg aattattgta agaaaaggat aaaaatagtt
      121 cagtccatct ccataattgt taaatgttgt atagttgatt ggcccttcaa aaatagaaga
      181 taaccctttg attcgtgaaa gatgaaaaga taaatcacct gttgtataaa tttcttgagc
      241 atttctcata aaatttactg aatataaagt actaagtaag ataagaacta ctaaaagaaa
      301 tgctttttta cttattttca tagatttgtt gttctccttt tttaattcta ttttcagtat
      361 actagaattg tcttttttta ataagttttg cgttaaattt ttcatagcac tagtatgata
      421 tactagaaaa gaatcaattt ttattacttt taaataggag aaaaaaatga aaaaaaacaa
      481 aaaccaaata atagatatca tttttatgtt atttctaagt tttgcttacg tcatccctct
      541 attcatgtct atgggaatct atcatagtgt caatcaggat acttactttc atttatctcg
      601 aatcatagga ctagataatg tatggagttc tccagtaaac ttcaataact tcgatcatca
      661 tggaacaatg atgaatattt tttatccatg gcttactctt tatccagctt ttttatttta
      721 taaaatgatg ggaaatcttg ttttaagtta taatatctac tattttttta tcactttttt
      781 aacaatggtc gtttcttatt tttctatgaa acaaataaaa aacaatcgtt atatttcgct
      841 gttattttcg attatttata cattttccgc ctatcgtgcc attgacatat tccatcgtgc
      901 ttcattggga gaagctgtcg ctttaacatt tttgcctttg atactaatgg ggtgttacga
      961 aatttatatt agagattacc aaaaatggta ctggttgtct attggcatga cgctagtagt
     1021 gtatactcat ttactttctg tcgctatggt ttctgtattt attggaggaa cgttgtttct
     1081 ttctttttat ttttgggacc aaaaaatcgc tagactctta agtcttttaa aagctaccgt
     1141 cctcaccttt tttttaagtg cgggattctt aattcctttt attcaacaat ctagagcaca
     1201 agaattgaaa gtcccactgg gaaaagaatt aagtggtatg gctcctagtg atatgctgac
     1261 acacatttta aataataact ataacaacta tactattgga ttattcttat ttctcggatt
     1321 gattggtgct tttattttta taaaaaaatt aactgctgat gatttattta ttttctttct
     1381 tggtgtattt gttctttttt gttcaaccaa tttatttcct tggcaacttt ttaaccatac
     1441 accagttaag agtctacaat ttgtctggcg gttaaatggt ttttcttctt tatttatagc
     1501 ctatacgatg agtatagtga tctattatat tttttctact aaaaataatt catggaaaat
     1561 aatggtcttt acgtttcttg cactaatatt gcatctctca ggagtaagca attctatcca
     1621 tgctaacaaa gattcattaa cacttatagc acctgaagac gctgttgcta tagctgaaaa
     1681 ttacaatcat actgattatg caaacaaaga gtcaatctat catcctgata tgattaataa
     1741 tgatcaatat ttactaggta accaggaaat aaatccaaca actcatttta tgagtaacaa
     1801 acttacaatt gaattagata attcgaataa taaaaatact gtcttgacga cacctattta
     1861 tcgttataaa gggcaagtgg cttctattaa tggcaaacta gtacaaacaa aattatctaa
     1921 gtttggcaca actgaattaa ctattcctcc aggtataaat aaagtcgtta ttacttatca
     1981 gtacacaaaa ctagcaattg cttcaagata tttatctatc gttactttga ttttattttt
     2041 actttatcga tttacttttt ctaaacaaaa acaaccgaga agagaagtac aacattctca
     2101 ctagattttt atttaagaaa gactttttag ctgtacacat aaacaggcta aaaagtcttt
     2161 ttttatattt ctttaatttg cttcatacat tatttaacat cgatatttat actgtgaaac
     2221 atgcgtttca tccattatca aagtattttt atcgataata aacttcatcc aaaaacagac
     2281 cttgtgcagg gacggtttct cctgcatgtt gccgcacttt gctttcaaat atttcatcga
     2341 ttgtttctat cggcatggca cctgtgccga tttctagtaa tgtccccatg ataatccgca
     2401 ccattttata taagaatccg tctccgacaa aagtaaaatg aagcatttct ccctttcgtt
     2461 cgatagataa ttcatcaatc gtacgcactg tggatttttt tgattttttc aatgcagaaa
     2521 aaccaatgaa atcatgtgta cccactaatt tttcgcaagc tgcattcagc ttgtccatat
     2581 ccaactcttg agggaaataa aagctgtggt ttcgctcaaa tgctgaagga atcacgttgt
     2641 tccaaacata atagctgtat tttttgccca ccgcattata tcttgaatgg aagcgctcag
     2701 gcatttcttc gattcttttg atgacaatat cccgcggaag gtaacgattc ataaagtcga
     2761 tcatttctgc cagtccaatt tctgtgtttg ttttaaaatt tgctacttgt cctcgcgcat
     2821 gggtgccagc atctgttctt cctgatccaa taatttctat ctcttcattt gtcatctgat
     2881 gcaatacttt ttcgattttt ccttgaatgg ttttatcgtc atctccaagt ctttgccatc
     2941 ctgaatatct ttttccgtca tattcgattg tcaacttgat gtttctcata ttttagtggc
     3001 tcctctgtct tccatttggt cttctctcta cctttttaag attgtagcac aaaatatagc
     3061 tcctttcttc tctaaaacag aagctataca agaaaactgt ggaaatatat atttcagaaa
     3121 aacaaaaaac agccaccgga tgaagtatcc gctgactgtt caatttgacg gctcaagttt
     3181 tactgtttga gatgcaagat actcaaccag gcaatgggca cataattaaa gaaaaaactg
     3241 attctttggc aaaaacctat tttattcagt aatggccaag aatttataga aggaattctt
     3301 gccagtccat aaatcgccgc aaaaagcaaa ctgatcacag acagtccaag gtataagtgg
     3361 cttacgtcca tttgcccatt ttttcgatag atcaagaaaa gaagaactgg aaacaataaa
     3421 aaaccactgt aacctaaacc tgatccgata ttatggaccc atgtcgagac attccacgaa
     3481 gattcatggg tattgatact gaacaaacct gtaaagatac aatcacctat accatagaac
     3541 gcgatggctg ctgtaactag ggtagctaac acacgagatg tacttacaaa tgcagcatac
     3601 aatgcaggca atgacaggat gaaaaagatc cctgaaacca cagaccaaat aagaaacgct
     3661 tttcttacag gactggaaac ctctccaaat acactgatca cgctcttcat ttgatctaat
     3721 tcaggataat acaatcctaa tacgtacgga gtcaaaaaat cactcagcac gcctaataat
     3781 aaaaagaaaa aaccaaattt acgtaaaaaa tccatgatac gcctccaata acaattacct
     3841 atgcttttaa ttgttattat aagcgacttt atcttgattt aacagaaacc tctatttaca
     3901 cgaacatcta tataactcat taaaaactcg aatcatttta aaaatactaa atatctaacg
     3961 ttcaacgttc acttaatcca tctaaacaat ctttcaattt gattttttcc ccacttcttt
     4021 tacgcgcgcc tagctctcct attgcagtaa acaacagatc acttgacgaa tttaaggcag
     4081 tttccattga atcctggata acaccgataa taaatccaat accgacgatt tgcatcgcga
     4141 catcattaga gataccaaac aaactacatg ctaatggaat caataacagt gaaccgcccg
     4201 caacacctga agctccacaa gcagctaaag cagacaaaat acataaaagg actttcaaaa
     4261 caaaaggtgc ttcaatccct aaagaatgta cagcagtcaa cgtcatcaag gaaatggtaa
     4321 tcgcggcacc gcccatattg atcgtagcac ctaacgggat cgatatggaa taagactctt
     4381 ttgtcaattt caatctttca gccaacatca tgtttatcgg gatatttgcg gcagaacttc
     4441 gggtaaagaa agcgggaagt gcgctttctt tcaaacaaaa gaaaatcaat ggatagggat
     4501 tttctttaat aaacaaaaac gtgaggaatg ggtacacaac taatgcgaca aacagcatcg
     4561 taccgatcaa aacaagtaaa agctgcagat actctgccaa tccagctatt ccagtcgtag
     4621 caattgattg ataaaccaat cctaaaatcc ctatcggagc aaatgcaata atcacttgta
     4681 cgacttgtga caacgctata gatatctgat ccaccaactc ttttgtcgat tctgaacgtg
     4741 ctcgaaaagc tagtcccaac aatacagccc agaataagac gccaaggtaa ttcgcatcaa
     4801 taagagattg aacaggattt tgtgtgacat tcgtcaacat agtagataaa acactttcca
     4861 aactttgcgg tgctttttca gatactgttt cttgtaaagt aataggaaca gtaaaaattg
     4921 aacttacaat aactgctaca agtgcagaaa gcaatgttcc cactaaatat aagatcaaaa
     4981 taggcttcac gaatgtctta ttcccaattt tatgcttggc aattgacgcc atcgtcaaga
     5041 aaaaaaccag aataggtgcg attgccttca agcaactgac aaacaacgtt cccaatattg
     5101 aaataaatgt ccatgtgggg aaaaagatac caaacaatat tccgactaaa ataccaacaa
     5161 caatacgttg aactaacgac atatccaaaa acttctctaa caaatgacca ccctttcaca
     5221 attttaacaa tcattgtatc attttatgca aagaaactaa aggtataatt ttctaaaaat
     5281 tctaacaata caaacaacca tttcttgcta attttgtact ttttctgtct acacgttgtt
     5341 ttttgagata ttctaagtta ggatgttatt aatgggcaac ttatcaggaa acgttttctt
     5401 atgtttttgt ccaatttttt tgtgaggtgt ttattcttga aaaaaataaa gaaggagagt
     5461 tatggcttcc tgacaatctt tgtcgcttgt ataatgatgg ttttacagcg tatattgatt
     5521 tcagcagtca ctattcctac atatgcaaca aaccctggtc gattctacat ctatacattt
     5581 ttacaaactt ccgttgttat tggtgcaaat ttgatcccac tttacatggg ttaccaatat
     5641 caaaaaatcg cccgtttaaa agttttacat tatttaagtc gttttgcctt tatattccta
     5701 atagccacac tagtttgcaa tatctttttt ttacgtacaa gctggcagct tgaatattcg
     5761 tgattattgg cttatcctaa cacctatcag tcagaattac tttacttatg ctgtttcttg
     5821 tactttgctt ttttgtagta ttccttatac agttggattt ttaaataaat tatcgaaaac
     5881 aacaattaaa tatctactat tatttttaac aattggatta gttggctgct ctactctatt
     5941 taataaagac ccgtttgcat tacagaatgg acagagtatt ttatggattt tttatttatt
     6001 tttaatgggt tatggtttga aagaatggaa ctggaaagaa aaggtccgct ataagcttcc
     6061 tcaagtattt atcagcttga ttgttttatt cggtttgatt attttgatga cacaaatttc
     6121 actcatcatc agaggaaata cagatacagc gctgaggttt tctaaaccat attctctctt
     6181 cgcaatgtat tatactttct cttcgttttt atttttagaa atgctatctg ataaattagg
     6241 tataaaagga agaggaaatt ttcttgctgt atttttgatt gtcactcaag ttatatgtaa
     6301 tttcccttta gtttcttatc gcgtatctac atttatgaaa cgtgaatttc caaactcagg
     6361 gaaggcatgg ctgataaata tcgggcagtt ttttatttgc tatttattag cagtgtccct
     6421 cctagttatc tttttatttt atatacaaaa aatccgtttc attcatcgtt tcatcgaata
     6481 cttcgctttt gattcgattg aacaattaac tcagcgttgg aaaaaaagaa ttcattggct
     6541 ttttgttcat aaaagttatt ttttagttgc agcatttttt tactgcttta ccttcgtgca
     6601 gattttcgtt ttagaaccaa aagaaggttg gaaagaaact atccaggttg ccttaaaaat
     6661 atttactcag cgacaagcac ccatgatcct gacaatcatc attattatgg gatttttctt
     6721 cttactattg ttgatttcca atcgtttttg gtacgccttg actgctacct tgatcattaa
     6781 cttactgctc actatttcga cagtaatcaa aatggaaatg cgggaagagc ctgtctttcc
     6841 ttcagattta aaaatactga ccggattgtc agaactatta tcgatggtta gcccagtctt
     6901 attaatcgtg ggcggattga tccttctcct attattgatt actagtatta ttgtacaacg
     6961 tagactacaa aaacagtatt ccttgaagat tcattggaaa cgtcgattta tcagtatcgc
     7021 tgtattatta ggatgttttt cgggtgtctt tttcattaat cacaaaaatt cgccttcttt
     7081 cttgctgttt aatttattta aagtaaacaa gactttcttt aaccagcaag gcgccgtaaa
     7141 agataacgga ccagtcattc aattcctaaa caatatcgac attaaaatca tggaagaacc
     7201 tgctggctat tcgaaagaaa aaattaatgg aataatgaaa aagtacaaca caaaggcaga
     7261 tgaaatcaac caaacaagaa gtgactggtt aaacaatcag acgatcgttc tcaatttaag
     7321 tgaaagcttc agtgatcctg cacgtgtccc taatctgaca gtacctacag accctattcc
     7381 ttacatcaaa agcattataa cagaaggaac aggaggattg atgttatccg tcggttatgg
     7441 tggcggaaca gctaacatgg aatgggaagg actgacaggc ttgagtatca gtaacctgtc
     7501 tgcttcgtta gttacacctt atacccagct agtggagcgt cagaaaataa gtccaaatat
     7561 taccgatcta ttcgatgaaa aaatagccat ccatcctttt acagctgctc tttatcgcag
     7621 aaaacaagtg tttgataaat ttggttttga caggttctat tatttagaca gtccggataa
     7681 attaacttat acggataaaa tagaacaaag cccacgcata tcggatgatt cagcatacaa
     7741 agaaaccttg aaagctttac aaagcaatac agaaacgtca caattcatcc aattatccac
     7801 tatgcaaaac catatgcctt atgatgatta ctatagtgaa ttgaactaca ctgctgaagg
     7861 aactgctgtc attcctaata gaagaaaaga attacaaact tatatgcaag gccttcacta
     7921 taccgataca gcagtaaaaa aattcatcca agaaatcgac aaaatcgata aaccgattac
     7981 ctttgttttt tatggtgacc atttacccgc aatctatagt gggaatgatt cgaaaaaata
     8041 tggattagaa cagcaccaga cagattattt catttataac aacaagtata gtcgtgaaca
     8101 agcagctacg ttaaataaag aagttgttgc tcctaataac tttggtgcta tggctcttga
     8161 gcatgcgaat atcaaagtaa ctccttacta tgcgttgttg acagaagtat ctaatcatct
     8221 acctgcatta acaattgatc ctacagaaag cttgtctaat cgttttaatg gaaaacaggt
     8281 ctttgttaca gaggatagtc aaattatcca agaaaaaaat ttaactgaga aacaaaaaga
     8341 aattctagaa gattatcgtc tgatacaata tgatcttgtt gcaggtaacc agtacagtgc
     8401 aacatgggcg acacaaaaaa t
//
LOCUS       DACEMS010000108         8419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEMS010000108 DACEMS010000000
VERSION     DACEMS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.111"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(48..1514)
                     /locus_tag="IW735_002222"
     CDS             complement(48..1514)
                     /locus_tag="IW735_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1045202.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="IW735_002223"
     CDS             1901..2497
                     /locus_tag="IW735_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1045203.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="IW735_002224"
     CDS             complement(2555..3037)
                     /locus_tag="IW735_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045204.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="IW735_002225"
     CDS             complement(3143..3340)
                     /locus_tag="IW735_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045205.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="IW735_002226"
     CDS             3635..4393
                     /locus_tag="IW735_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1045206.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="IW735_002227"
     CDS             4453..4881
                     /locus_tag="IW735_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045207.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="IW735_002228"
     tRNA            4927..4999
                     /locus_tag="IW735_002228"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="IW735_002229"
     CDS             complement(5149..5520)
                     /locus_tag="IW735_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1045208.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="IW735_002230"
     CDS             5735..5920
                     /locus_tag="IW735_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045209.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="IW735_002231"
     CDS             complement(6003..6191)
                     /locus_tag="IW735_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045210.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="IW735_002232"
     CDS             complement(6215..6478)
                     /locus_tag="IW735_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045211.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6636..6893
                     /locus_tag="IW735_002233"
     CDS             6636..6893
                     /locus_tag="IW735_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1045212.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="IW735_002234"
     CDS             complement(6964..7155)
                     /locus_tag="IW735_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045213.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7296..7616)
                     /locus_tag="IW735_002235"
     CDS             complement(7296..7616)
                     /locus_tag="IW735_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045214.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7639..8073)
                     /locus_tag="IW735_002236"
     CDS             complement(7639..8073)
                     /locus_tag="IW735_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1045215.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2672 a         1656 c         1330 g         2761 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac tcctcgaact
     7261 aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat ttcattctgc
     7321 tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata atcatagctg
     7381 tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc gttagctgtg
     7441 acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac accttttttt
     7501 cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc gtcttcaata
     7561 attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg aaccattttc
     7621 tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat gtgatacgac
     7681 ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg tcgctcccat
     7741 aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat ttcttggatc
     7801 tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat cattgatttt
     7861 tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg aaaaggcata
     7921 ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt gggcttcatt
     7981 gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg atccgtcaac
     8041 tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt tcttttattg
     8101 taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat gatgaacaaa
     8161 agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct gttctaatcc
     8221 cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga acatcattct
     8281 gttacaaaaa acgtggattt gtcataaatt gtcatgcttc ttttacctat gaaatatttt
     8341 cacgtgttaa agtattcctc gttgaacaca acaaataaaa ctttttaacg aataaaggag
     8401 atttttcatg acatcactt
//
LOCUS       DACEMS010000109         8287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEMS010000109 DACEMS010000000
VERSION     DACEMS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.112"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            213..1406
                     /locus_tag="IW735_002237"
     CDS             213..1406
                     /locus_tag="IW735_002237"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1045216.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1536..3008
                     /locus_tag="IW735_002238"
     CDS             1536..3008
                     /locus_tag="IW735_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1045217.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3091..3879
                     /locus_tag="IW735_002239"
     CDS             3091..3879
                     /locus_tag="IW735_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1045218.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3946..4614)
                     /locus_tag="IW735_002240"
     CDS             complement(3946..4614)
                     /locus_tag="IW735_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1045219.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4729..6441
                     /locus_tag="IW735_002241"
     CDS             4729..6441
                     /locus_tag="IW735_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1045220.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6444..8216
                     /locus_tag="IW735_002242"
     CDS             6444..8216
                     /locus_tag="IW735_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1045221.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2539 a         1387 c         1822 g         2539 t
ORIGIN      
        1 aaaagcttag cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa
       61 tgtaaaatat ttttatattt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
      121 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
      181 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
      241 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
      301 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
      361 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
      421 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
      481 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
      541 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
      601 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
      661 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
      721 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
      781 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
      841 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
      901 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
      961 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     1021 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     1081 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     1141 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     1201 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     1261 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     1321 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     1381 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     1441 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     1501 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     1561 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     1621 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     1681 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     1741 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     1801 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     1861 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     1921 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     1981 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     2041 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     2101 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     2161 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     2221 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     2281 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     2341 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     2401 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     2461 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     2521 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     2581 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     2641 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     2701 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     2761 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     2821 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     2881 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     2941 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     3001 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     3061 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     3121 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     3181 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     3241 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     3301 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     3361 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     3421 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     3481 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     3541 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     3601 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     3661 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     3721 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     3781 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     3841 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     3901 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     3961 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     4021 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     4081 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     4141 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     4201 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     4261 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     4321 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     4381 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     4441 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     4501 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     4561 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     4621 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     4681 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     4741 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     4801 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     4861 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     4921 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     4981 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     5041 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     5101 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     5161 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     5221 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     5281 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     5341 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     5401 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     5461 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     5521 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     5581 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     5641 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     5701 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     5761 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     5821 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     5881 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     5941 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     6001 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     6061 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     6121 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     6181 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     6241 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     6301 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     6361 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     6421 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     6481 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     6541 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     6601 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     6661 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     6721 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     6781 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     6841 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     6901 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     6961 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     7021 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     7081 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     7141 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     7201 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     7261 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     7321 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     7381 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     7441 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     7501 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     7561 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     7621 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     7681 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     7741 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     7801 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     7861 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     7921 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     7981 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     8041 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     8101 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     8161 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     8221 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     8281 ggcagaa
//
LOCUS       DACEMS010000110         8061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEMS010000110 DACEMS010000000
VERSION     DACEMS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.114"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(9..530)
                     /locus_tag="IW735_002243"
     CDS             complement(9..530)
                     /locus_tag="IW735_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1045222.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="IW735_002244"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="IW735_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1045223.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="IW735_002245"
     CDS             4610..5497
                     /locus_tag="IW735_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1045224.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="IW735_002246"
     CDS             5494..6663
                     /locus_tag="IW735_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045225.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="IW735_002247"
     CDS             6687..8042
                     /locus_tag="IW735_002247"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1045226.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2591 a         1486 c         1828 g         2156 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa a
//
LOCUS       DACEMS010000111         8051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEMS010000111 DACEMS010000000
VERSION     DACEMS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.115"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(113..1066)
                     /gene="menA"
                     /locus_tag="IW735_002248"
     CDS             complement(113..1066)
                     /gene="menA"
                     /locus_tag="IW735_002248"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1045227.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1079..2182)
                     /locus_tag="IW735_002249"
     CDS             complement(1079..2182)
                     /locus_tag="IW735_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1045228.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2272..3207)
                     /locus_tag="IW735_002250"
     CDS             complement(2272..3207)
                     /locus_tag="IW735_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1045229.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3509..5443
                     /locus_tag="IW735_002251"
     CDS             3509..5443
                     /locus_tag="IW735_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1045230.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5900..6316
                     /locus_tag="IW735_002252"
     CDS             5900..6316
                     /locus_tag="IW735_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1045231.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6330..6896
                     /locus_tag="IW735_002253"
     CDS             6330..6896
                     /locus_tag="IW735_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1045232.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6919..7905
                     /locus_tag="IW735_002254"
     CDS             6919..7905
                     /locus_tag="IW735_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1045233.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
BASE COUNT         2401 a         1564 c         1623 g         2463 t
ORIGIN      
        1 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta tttttatttg
       61 gctgttctta ttacttgatg cagggcagtt ttttcacaac ctcttcatgc tgttaaatgg
      121 cttgcataat gattgttagt aatagcccca atacataact ggcattgaat aagaccatat
      181 ttttgatgga atagctgaaa ctgataggct ggggtaacgt ttgcttgaat tgatccaaat
      241 gcttcttcac aattggcaag gtcaagaagg tgaccaagat cggccattca tagataccgc
      301 tcaacaaccc cattaaaatc acgaaatagc aagcatacat caatagctgg aataagactg
      361 ttcccgcagg acgtccgata taaaaaacta gtgtatagcg atgattttca atatcacggt
      421 caagatcccg caaattatta gccaacatga tattggcaat cgtaaacacc aagggtaagg
      481 aagctaatac gattgctgct atagcccaaa gatttcctac caaatcaaat tgtccagatg
      541 aaaagttgac tgtcagatca tagacatctg aaccgaatgt attgacataa acgaccatgg
      601 caaaaattcc aaggcccata gtcaccccac tgaaaatttc ccctaaaggc attcgagata
      661 aaggaatcgg tccgaatgta tagaatactc caataaaaca acagataaat cccatcaaaa
      721 gtaaaagcca acccgtattg atcgttagaa taacgccgat aacaaaaaca aatgcaatca
      781 tagctaaaat catgtttcga atcaatacag gagatagatt tctgcggcca atgacatttt
      841 cttcatactt atatacttca gaatgagcct tttcataatc catgtaatta ttgatagcgg
      901 tagtcgctaa atcaaaaact agcattccaa taaaaaataa aaaagtgttt agccaatgta
      961 tctcatgaaa aaaagcgata gagaataata cccctaccgc aaaaggaaat acactagcca
     1021 cctttgttcg tatttcaact acttctaaaa agatttttag tgtcatggtt cgtactcctt
     1081 attttagttc agaacggtca cccatcgtgt aatgggagtc ctccgataat ttgaaggtgt
     1141 ctttgattgg atcggtcaca tatactttat cgtcttttgt cacgaagatc gcttccgtcc
     1201 cgttattcaa ttcgctttcg acgtattcca gtcctttttt cacacccatt gaaaagactg
     1261 ctgtagacaa cccgtcacca tcaattgatt tatctgttat gatcgtgaca cttgcgatat
     1321 cattgtcgaa cggataaccc gtttttggat caaacaaatg atggtatttt ttgccatcta
     1381 cttctaaata acgctcgtaa attccagagg taactaatgt tttgttccgt tctttaatag
     1441 aacctaaaac agatccgcga gccatgttcg gatcttggat gccgactgtc cagtcttggt
     1501 tttcgcctct tgggctgtgg cctaagacat agacattacc gcccaagtca acgatggcag
     1561 tggtcactcc ttgttttttc aagactttca ctacttcgtc cgtaatgtaa cctttggcga
     1621 ttgcacctaa atcgatgatc attccttttt cttttagata aacagttttt tctttgtcat
     1681 ttagttcgat cttatgataa tccactaatt tcaatgcctg atcgatttct tcttgggaag
     1741 gttttctggc gtcatcaaaa ccgattcgcc ataattgtgt gattgcacca atcgccatat
     1801 cgaatccgcc ttgagaatct tggctatatt cgtaggcccg cttgactaac gtgtagacgt
     1861 catcagaaac ttttactggt ttgatcccag cttgctcatt cacttcatcg atctcagaac
     1921 ctttttgatt gatcgtgatt ttatcaccaa gttcttttac tcgatcgaag gcaggtttca
     1981 atgctgattc tttgccttca tcatacacac ggatacgaac gtaagtccct aacaagaact
     2041 gttcatcaga atatggatct ttcaatatct tggtagctgc tgaactttca gtggttgttt
     2101 tatttgctga gttgctgcag ccagttagca ccataaaaga gatgcccagt gtgattaagg
     2161 aaaatagtag ttttttccgc atctttttcc tctcttcatt ttgaaaggtg agacgactgc
     2221 ctaatctggc tatcgtccca ccctccaact tttattagta tgattatgtg attatttttc
     2281 tgttacgata ttatctactt cgattgtttg agtgtcgcct ttttcagctg cattcactaa
     2341 ttgttgtgcg tacatgatga atgaatgtga gctgtgtgta gcacctgtaa caacttctac
     2401 attagcaggt gagccatctt cttcgcccat tgctttgacg aattcgtcgt tcagagtagg
     2461 gatgtaagtt tttggttctg taccgctttt tgctttcata ttttcattat attctgtatc
     2521 atcttgtttt gatttaccgt ctttgtcgat ataatcaaag tttgattcag ttactttgcc
     2581 gccagcaact gtcatttcga attgcacacg gtagccgtta gagtagttct tttcttcaag
     2641 cttgtaagtg ccgtctttca aatcagcacc attgtcgatt tcgattgtat ctgtgttacc
     2701 agcttgagca gcttggatca attgttgtgc gtagttttgg aagctttcag atgagtgagt
     2761 agctccagtt acgacttcca caccgcttgc gctttgtgct tttaagaatt ggtcattcaa
     2821 ctcaggaatg tatttttctg ggtttgtgcc agattttgct tccatgtttt tgttgtattc
     2881 tgcatctttt gtttttgatt cgccgttttc attgacgtta tcatattttg attcagtgat
     2941 tttgccgtct tttacaacca tttcaaagac tgcacggtaa ccatttgaat aatttttttc
     3001 ttctaattta tatgtgccgt cttgcaaatc tccgccagct acaacttttg cagatgattc
     3061 tgttgtgctt tttgcagtcg aagattgtgc tgtagatgat tcagaagaag agtttgagct
     3121 tccgttgtca tctgcgccgc aagcgcctaa cactagagct gaaaatgcta atacggcaaa
     3181 tccagttaca aaacgttttt tcttcatttt aataccccac cattccattt ctagtccaca
     3241 atttttcttt gtgattcatt atacattcta atctttttag cctataagtc aactattttt
     3301 gataaataac ccatgtaata tttcaaacgt tttctttcag caaatttcgt gaaaaaacgc
     3361 tttattttaa aatttgaaaa aaatccaaaa ggagttagac aaaagaacag aacatctata
     3421 taatatatgg agattgtgta ataagtatca aactaaaaga attatcatat attcattata
     3481 tagatagtct ataaggagcg gatgatcaat ggcaaagcaa aatattgtcg ttgtaggagc
     3541 aggatatgcc ggtgtttcag ctacaaaata cttggcaaag aaactgaaaa aagaagatgt
     3601 agcaattact ctgatcgatc gtcattcgta ccatactatg atgaccgagt tgcatgaagt
     3661 agctggcgga cgtgtagaac cagaagcaat ccaatacgat ctgcagcatt tgtttgctcg
     3721 tcaaaagaat gtacaacttg tcacagacac tgtaatcggt atcgataaag aacgaaaaat
     3781 cgtcaagacc aaattaggtt cctatccttt tgatcagctg atccttggaa tgggcggaga
     3841 gccgaatgac tttggtacac ctggtgtcaa agcacacgga ttcacccttt ggtcatttga
     3901 agattctgta aggattcggg aacatattga gaagacagta gcaaaagctg cattggaacc
     3961 agatgatgaa ctgcgtaaag ccatgctgac tttcgttgtc tgtggttctg gatttaccgg
     4021 tattgaaatg attggggaac tgatcgactg gaaaaatcgc ctagccaaag attataaatt
     4081 agatccaaac gaaatcacac tgatggtcgt agaggcaatg ccgacgatct tgaatatgtt
     4141 ggatcgaaat gatgctgcaa aagctgaacg ctatttgcgt aagcacaacg tggaattatt
     4201 attgaatgca ccaatcgtag aagtggctgc tgatcatatc aaattgaaag acggacgcaa
     4261 cattccgact cacacactga tttggacagc tggtgtcaaa ggaacttctg acgctgctga
     4321 ctttggatta gaatcagcac gcggacagcg gttgatcgcc aatgaatata tgcaggcaaa
     4381 aggctacgaa gataaaaaca tctatatcat tggtgattta gtcttttatg aagaattccc
     4441 aggaacaccg acaccgcaga tcgttcaagc tgctgaacaa acaggacaca cagccgcagc
     4501 gaatatcgtg gcttcgatca aaggcggaga aaagcataaa tttaaaggaa actatcaagg
     4561 atttatggtt tctattggtg ccaaatgggg ggtagctaac ctatttgata agatccattt
     4621 aagcggattt ttagctatcg taatgaaaca tatcgtgaat ttgaaatact tctttgatat
     4681 tcgttctggt tattatatgt tccaatattt aatgcatgag tttttccata tcaaagatga
     4741 tcgcaacgtg actcgaggac attcttctcg ttacggcaat gtcttatgga gtgtgccact
     4801 tcgtattttc tatggtttag tttggttcgt tgaagcttcc aaaaaaatca ttgggaatgg
     4861 tgaagtgttc aaaccaagta cttggtttgg tgaaggatca tggttcactg gaaacagtgt
     4921 gttcccttgg acatgggaat acaatgtaac agatgttgcc agtggtgctt ctgctgctgg
     4981 tgatgcaacc acagctgcca gcggagcagg ggcaagtgcc ggagaagcag cgactcaagc
     5041 tgcacatttt ggtttgagtt atgcatacgg tgaagaacca atggctgtga ttgagaagtc
     5101 accagactgg ttcacaaaga tcatgcaagt gatcatgcca aacaatgatg tagcaatgtt
     5161 cttccaaaaa ttcatggtat tctttgaaat tgccttggca ttagcattga ttgctggatt
     5221 attcacatgg ctatgcagtg cgacaacgat tgtattggtg atcacgttct gcttatcagg
     5281 catgttttac tgggtaaaca tttggttcat ttttgttgct ttcgcattga tgaacggttc
     5341 cggacgtgcg attgggttgg atcgctgggt aattccatgg ctacaacgta cactaggtaa
     5401 ttggtggtac ggtagaccaa gttcccagta cggcaagaaa taaatagata aagaacgtta
     5461 tttgaatgca agatttcaat agaaaggggc tgagacaaac cgtcacagcc tcctttttat
     5521 gtaaaattaa aggaataaga tgcccgagat aagcctccat gagcaagaca caggtgtcac
     5581 aacctctgtt acaacctctt gttataaacg gtgttccaga agtagcttct tcgaaaaata
     5641 agccgaaatt tcacaaaaac ttgaaaagta attttcgtaa attccttctt atttcttgaa
     5701 gttgcccact tctgtcacaa cctcttgtta taaacggtgt tccacctgct gcttctcggt
     5761 aataagccca aagaattaga aaacatggaa agctgttttc tattcttcgt tcttattact
     5821 caaagcaaga cacaggtgtc acaacctctt gtttttatgc taaaattggg tgagttaatc
     5881 tggaaaaagg agaacaaaaa tgagccttaa agaatttgct gaaaaaagtc tgctcaaacc
     5941 ttgggatgtt tttattattt tgcttttgac tgttctttct tttttaccag tcatgatttt
     6001 tggtcttcag caaaacaccg caacagcaga aaaagaagcg gtactgcgtg tggatggcac
     6061 agaaatcaaa acattccctt tgatggctgg agaagagagc aacacatata cttacaaaga
     6121 tgaacacgga gattataact tgatcgagat agatggcgat cgagtacgga taaaagaagc
     6181 agattgcgat gaccagatct gcgtacgtcg aggctgggca acgaaaaatg gcgaaaccat
     6241 cgtctgtttg ccacataaat tagtgatcga agtccgttca acagatggag gtgacgaaga
     6301 cagtctgatt tattagacct tcacacacca tgagtaaatt acaaaaaatg atttatattt
     6361 cgctacttgt tgcacaagga gtgatcattg gtttattgga aaatatgatc ccttatcctt
     6421 tttcttttgc gcctggtgca aaactaggct tagccaatct aatcacgatc gttgccattt
     6481 ttaccatgaa aaaacaagac agcttcttac tactttggct acgactattc ctgactactt
     6541 tgctaggcgg aacgatttcc acattccttt atagtatgag tggtgctttg cttagttatt
     6601 ttggaatgtt attggtcaag caattaggac caaaacgtgt cagtatcatc ggaattagtg
     6661 cggccggcgg tttcatgcat aatgtcggac agcttttgat ggcttctttc attgcacaat
     6721 catggacagt catgttgtac ttgccagtcc tttcagtttt agggatttta tcagggattg
     6781 caatcgggat cgcagcgaat tatcttttac gacatgtgca gacacttcaa cgattccaaa
     6841 aagattatga gcagggaacc aaacaaaaat ggagcccaat gtttaccaaa aaataatcgt
     6901 cacacaagga gtttttttat gaagctacat ccaatgtggg aaaactatcc tgctttaagt
     6961 aaagaattga cagaaacact aaaacggatg gaagaaactg tccgcctgaa aaacaaacct
     7021 gtcgaacaag caatcaaaga aacgatccat gccggcggaa agctgttgcg tcctgcctat
     7081 cagctattgt tctcacaatt tggaccagag caagatcgag acaaagccat cgctttggca
     7141 gcttctatcg aaatgctcca tacggccaca ctgatccatg atgatatcgt tgatgaagcg
     7201 gatctgcgac gtggtcagcc taatatccgt agtcagtttg gtaatgtcgt ttctgtctat
     7261 gcaggagact atttatttgt ctgctgtttt aaactattgt ctgattactc cacttccttg
     7321 aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat tgaatggcga attagggcag
     7381 atggatgatc gctataatta tgaactttct gtaaaagaat atttggaaaa tatttcaggt
     7441 aagacagctg aactgttcca attaagttgt tctgttggcg cttttgaaag tggaacaagc
     7501 gagcgcttcg caaaaaaagc aggagatatc ggactttcta tcgggatggc ctttcagatt
     7561 atcgatgata ttttagatta tacaaaagag agccaggaaa tcggcaaacc tgtactagaa
     7621 gacatgcgtc aaggggtcta ttctttaccg ttgatctact ctttgcaaaa aaacaaacca
     7681 aagctactac cttatttaga gaagaaagcg gcaatgacag aagaagatgt cgacgccgta
     7741 cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta ggaagttagc ggcctcttac
     7801 acagaaaagg ctttgaagga aataaagaaa ttacctgcca cctctcttcg aacaaaagag
     7861 aatctctttt cccttaccca actgattttg gatcgcaaag actgaatagg gaaaggttcg
     7921 atacgtaaat aaatttctca gaaggaaatc tacgtgtttt ttgacaaatt gaattgtagt
     7981 tcttgtattt atgataaaaa gcaaaaataa agagcaacgt ccgttagcga cgttgctctt
     8041 ttttatcttg a
//
LOCUS       DACEMS010000112         7759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEMS010000112 DACEMS010000000
VERSION     DACEMS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.116"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            145..1092
                     /locus_tag="IW735_002255"
     CDS             145..1092
                     /locus_tag="IW735_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1045234.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1104..2024
                     /locus_tag="IW735_002256"
     CDS             1104..2024
                     /locus_tag="IW735_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1045235.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2065..3522
                     /locus_tag="IW735_002257"
     CDS             2065..3522
                     /locus_tag="IW735_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1045236.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3867..4481
                     /locus_tag="IW735_002258"
     CDS             3867..4481
                     /locus_tag="IW735_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1045237.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYSSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4474..6198
                     /locus_tag="IW735_002259"
     CDS             4474..6198
                     /locus_tag="IW735_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1045238.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6195..7646
                     /locus_tag="IW735_002260"
     CDS             6195..7646
                     /locus_tag="IW735_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1045239.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2618 a         1290 c         1543 g         2308 t
ORIGIN      
        1 gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt
       61 tcattttccc tatactttgt ttatttattc tcattattga aagcgctata attacgttgc
      121 gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg
      181 aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt
      241 tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca
      301 gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa
      361 tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta
      421 gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg
      481 agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct
      541 tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat
      601 cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg
      661 ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac
      721 tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct
      781 agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt
      841 ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt
      901 cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat
      961 ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt
     1021 gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct
     1081 gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct
     1141 ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct
     1201 gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga
     1261 ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg
     1321 accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag
     1381 gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact
     1441 ttccattccg tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga
     1501 tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat
     1561 tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac
     1621 gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa
     1681 tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg
     1741 ccgcattggg ttactggaat gactggttca atgctttact atacatccaa gatgataagc
     1801 tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa
     1861 attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca
     1921 gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga
     1981 agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa
     2041 atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt
     2101 tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca
     2161 gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc
     2221 gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat
     2281 atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg
     2341 gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt
     2401 gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa
     2461 gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat
     2521 gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata cgatctttca
     2581 attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa
     2641 gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat
     2701 tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc
     2761 atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac
     2821 aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga
     2881 aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg
     2941 acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca
     3001 gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca
     3061 gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg
     3121 atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat
     3181 ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat
     3241 acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca
     3301 gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt
     3361 aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca
     3421 gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg
     3481 aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag
     3541 gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca
     3601 gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt
     3661 tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag
     3721 ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt
     3781 tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta
     3841 taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc
     3901 ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga
     3961 tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat
     4021 tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa
     4081 gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat
     4141 attcatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg
     4201 ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga
     4261 tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt
     4321 ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat
     4381 tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt
     4441 ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat tctattttca
     4501 aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg
     4561 atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa
     4621 aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg
     4681 agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc
     4741 tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc
     4801 gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta
     4861 agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac
     4921 aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg
     4981 cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa
     5041 ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct
     5101 ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa
     5161 agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat
     5221 ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct
     5281 gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt
     5341 ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat
     5401 tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag
     5461 acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac
     5521 gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag
     5581 caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca
     5641 ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg
     5701 tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa
     5761 gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga
     5821 gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg
     5881 atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat
     5941 gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat
     6001 ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa
     6061 gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc
     6121 ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt
     6181 acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt
     6241 tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag
     6301 cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa
     6361 cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac
     6421 gtcataattt tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag
     6481 gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg
     6541 aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag
     6601 aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg
     6661 aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg
     6721 caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact
     6781 atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg
     6841 atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg
     6901 gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc
     6961 tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag
     7021 aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat
     7081 tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga
     7141 tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga
     7201 ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg
     7261 tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt
     7321 acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta
     7381 cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca
     7441 aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga
     7501 aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca
     7561 acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt
     7621 accgagaaaa cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa
     7681 aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc
     7741 atgacctctc gataaacgg
//
LOCUS       DACEMS010000113         7623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEMS010000113 DACEMS010000000
VERSION     DACEMS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.117"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(14..706)
                     /locus_tag="IW735_002261"
     CDS             complement(14..706)
                     /locus_tag="IW735_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1045240.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IW735_002262"
                     /pseudo
     CDS             791..967
                     /locus_tag="IW735_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IW735_002263"
     CDS             complement(1143..1712)
                     /locus_tag="IW735_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1045241.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IW735_002264"
     CDS             complement(1786..2109)
                     /locus_tag="IW735_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045242.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IW735_002265"
     CDS             complement(2263..3075)
                     /locus_tag="IW735_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045243.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IW735_002266"
     CDS             3429..4277
                     /locus_tag="IW735_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1045244.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IW735_002267"
     CDS             4422..5363
                     /locus_tag="IW735_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1045245.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IW735_002268"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IW735_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1371 g         2469 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DACEMS010000114         7596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEMS010000114 DACEMS010000000
VERSION     DACEMS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.118"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(134..424)
                     /locus_tag="IW735_002269"
     CDS             complement(134..424)
                     /locus_tag="IW735_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045246.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(542..802)
                     /locus_tag="IW735_002270"
     CDS             complement(542..802)
                     /locus_tag="IW735_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045247.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(939..1352)
                     /locus_tag="IW735_002271"
     CDS             complement(939..1352)
                     /locus_tag="IW735_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1045248.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1808..2293)
                     /locus_tag="IW735_002272"
     CDS             complement(1808..2293)
                     /locus_tag="IW735_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045249.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2432..2677)
                     /locus_tag="IW735_002273"
     CDS             complement(2432..2677)
                     /locus_tag="IW735_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1045250.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2870..3670
                     /locus_tag="IW735_002274"
     CDS             2870..3670
                     /locus_tag="IW735_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1045251.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3689..5557
                     /locus_tag="IW735_002275"
     CDS             3689..5557
                     /locus_tag="IW735_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1045252.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5550..6041
                     /locus_tag="IW735_002276"
     CDS             5550..6041
                     /locus_tag="IW735_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1045253.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6029..6583
                     /gene="srlA"
                     /locus_tag="IW735_002277"
     CDS             6029..6583
                     /gene="srlA"
                     /locus_tag="IW735_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1045254.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6601..7596
                     /locus_tag="IW735_002278"
     CDS             6601..7596
                     /locus_tag="IW735_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1045255.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1214 c         1441 g         2233 t
ORIGIN      
        1 ttactatgaa taacataata agaaagtaaa gtggattgat ttgagatctt tttatacttc
       61 aaaaaagata acacccaact ttcaaaaata tgttttggaa gttgggacaa acgagttaat
      121 acattttaat acattatttt ttattcaaaa aaaatagcac aatggcaaag atcgtaagag
      181 agatcataag ccccaatgaa tgactaaaaa gaaaagcaga atcacatgcc atatcatccc
      241 atatttcttc aagaaaataa ataagcggga aaaagggaag gaatagtaga acatcgatcc
      301 agcaccattt ttttcttctt ttgaaataac ataaaacaat aaacatgaac gtgcagggaa
      361 gtaaaaacaa agctttactc ataactaaca aaataccagc tccttttatt tttttctccc
      421 ccacattcaa agagtaacaa tggtatgtac ttaataatag aaaaatggat caacttgtat
      481 gaataacagc tgaaaagatc tgcaccaaat aaaaaacttc cagccaaaac tgactggaag
      541 tttatttttt tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc
      601 cgatttcgta gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat
      661 ggataaaata gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata
      721 agtaaaagaa agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga
      781 caatcaaatg atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa
      841 aaacaaaata tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat
      901 ataaaaatcg cccctcattc atcgagaggc gataagttct aatttaactc aatttttttt
      961 gtttcttttg gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag
     1021 gttccagtga ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc
     1081 gaatgtcttt ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga
     1141 ttggcggaga tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag
     1201 ccaggcatat cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga
     1261 taacttgaaa cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg
     1321 aacatgtcgc gaatgctagg tacatttgcc atttttgatc atcctttcaa taagtatttt
     1381 ttatttatcc ctatttacgt aataactcta ttttgaaaaa aatgcaagta taatggttag
     1441 ctttgtgaat atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat
     1501 cattaaaata ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa
     1561 tatagaaaaa ttgaatctag aaaaccgccc gacaataaat ttcattaact cagttgaccg
     1621 aaaatgaagt agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat
     1681 aagagaaata gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata
     1741 actttctcag aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat
     1801 catctaatca aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca
     1861 ctcgataaaa accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg
     1921 tgaacaaata atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta
     1981 attcggaacg gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag
     2041 aaggtacagt gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg
     2101 attggctatg ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag
     2161 tacttttagt tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat
     2221 tctttgtttt atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa
     2281 tccctttatg catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa
     2341 acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga
     2401 agataataac ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa
     2461 tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct
     2521 tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc
     2581 ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaagt
     2641 tttcattaga attctccttt cttaaatgat aaaacaatta tatatcaaaa agagaaacat
     2701 tgcttatata acgctcagga attttagcaa tctatgcaaa aagatgaaaa ttttttttgc
     2761 tcttccatta gtacacattt tttagctgaa gctgtaaatt tcatgtctta atttttttta
     2821 gtgagctttt atactggatt tgtaagaaaa aagaaggaga ggatcacata tgtcggattg
     2881 gttggatatt gcaggaaaag tagtaattgt aactggaggt tcctcaggaa ttggaagaag
     2941 tatcgtcgaa aatttgttga agcagaatgc acaagtagcg aattttgatg taactgaatg
     3001 ccgtatacag catgaaaacc tactatcttt aaaagtcgat gtgtcttcta aaacggacat
     3061 agaagaaggg atatataaag taatgaaaca tttcaagacg atcgatggtc tagtcaataa
     3121 tgcaggaata aatattcctt ctcttctaat agatagaaat catccaaaaa gcaagtatga
     3181 attatcagaa caagtttttg ataagatgat cgctgtcaat caaaaaagtg tatatttgat
     3241 gagccaagca gttggaagaa tcttggtgca aaagggatca ggagtgatcg tgaatctttc
     3301 ttctgaatca ggattggaag gatcagaagg acagagctgt tatgctgcta caaaagcagc
     3361 gatgaacagt tttactcgtt cttgggcaaa agagttagga aagaggaatg ttcgtgtggt
     3421 aggtgttgca ccagggatac tggaagaaac cggtttgaga acacaagaat atgaagaagc
     3481 tctttcttat acgagaggta taagcgtcga acagctgaga aacggttaca gtcgaacaag
     3541 tacgatccct ctaggaagga gtggtaagct gcaagaagtg gcggatctag tctgctatta
     3601 tctttcagaa agatcaagtt atatcactgg agtcacgact aatatttcag gaggtaaaac
     3661 aagaggataa aggatagggg ggacgcacgt gacgctagta aacagatggt accaattatt
     3721 gaaattacta gtcactcaca agaaagtttc aatccaggaa gtgcaaaaag aaatcaaaac
     3781 cagtaatcaa accataaaaa aagatattgg ggagttgaat gaagaaattg ccggtattgc
     3841 aaaaatcatc caaaaaaaca aacattttga acttgaaatt tatgattttg atttttttga
     3901 tgagatcatg caaggaaaat tgaaaacagc cagtgatttc aattcggcat ccaaaagaat
     3961 cgcttattta ctaaataggc tgatcgaaac agaggatttc ttacgtatgg atgatttgtc
     4021 tgaagaagtc ggtgtcagca gaggaacagt ggtcaaggat ctaaagacat taaaagagat
     4081 gatcaatgat tttgatgtaa agattacagg cacaccaaat aaaggactaa gaataatagg
     4141 agatgagcta gaattacgac tacttttatt ttattttgtt tctccctact tttcagagac
     4201 gaaaacagaa cagtttctag aagaaaaaca agcaatcaaa cattttcaga agaagacgaa
     4261 tgctagtaaa caagaaatca cccttttaac gaaagttatc ggtatttctt tgaatagaat
     4321 ctctagtaac tatctgttga agaagcccat caatcgttac agtgggtgtt ttgaatatat
     4381 ccaagaattc aatgaactga tcaatcagtt ggaagaaatc tatgaactaa ctttgagcca
     4441 atatgaaaaa gattttatca gttttccttt gaacatcatc aacacaagta taacgatgat
     4501 aaggaaagct aactctgatt tgcgcttata tttcgacccc atgatgatgc gtattcgacg
     4561 attagatctc attgatctgg atgagtcctt cttatatcaa gagatgaaac agcacctctc
     4621 tttattgata aatcgggtaa tttttcgtta tcgattgact gatttatttt atggtgagat
     4681 agaaaaaaag tatccttttt cttaccaaat cgctagcgaa tgcatacaag cattagaagc
     4741 tatcttgcaa cataagatct ctaaagtaga gatcagctac ctaacgttgt atttggagtt
     4801 agcatttcga tatcaaaaag agcatttttc ctctaaaaaa gtagccattg ttcatacgac
     4861 aggaaaagga acagcattaa tgatccaaag acaaatcaaa aggatattgg ggagggatat
     4921 acaaataaca catgtttcag aagaacaata tgaaaaagta gatctaaaca aatatttcgc
     4981 tgtttttaca acgattccgt taaaaaatgt aaatgaagag acaccggtga tccatttgac
     5041 taatcttttt gatgactctt ggttaagggc acaatggcaa aaagcaaaaa aaaagcgatt
     5101 agccgggttt cattctactg tttttttcta tcaaatatgg aaagaaaaaa agacgtatca
     5161 agaatattta aacaaattag tcgctccttt gatcagaagc tattatgtgg atgaagaatt
     5221 tgaaaaaagg atcttgaaaa gagaatatca gcaaagcaca tttgttaaca aagaatttgc
     5281 ttttcctcac acaaccaacc agctatctca acacattgtt ttatcagtag ggctaataac
     5341 tgatcaaggt ttagcagaaa caggaggaaa tggagttatt tttttatttg ccatacctga
     5401 agaaatgacg gctgaagttg agaatgaatt atttgagcta tacgatatcc tatttgaact
     5461 cataaaaatg ggaatagatg agtttattga attagcaccg acacaagaat catttttaaa
     5521 actaatcaaa agtaaaggag agagacttca tgaatgatat gtatctattc atagttttcg
     5581 cagcttcagc ttatgtgctg caagcagtta tcagttggaa gcaactaaag catttcaatc
     5641 aaacatatag cgaactgcga agaaaaggaa aagtagcgat tggtaaacga cctgggaaaa
     5701 tccgttcagg cacgattgtt atgctagcta tagacgatca tgatcaagtt ttagaagtca
     5761 gaaaaatgca aggggttaca tctttggcaa aatttcattc attacctgtc tatgcgggtg
     5821 aagatatcca ttacatcgat cgttaccatc cctcagttcg agcggaagat cgtttgacac
     5881 aagcagcttt agaaaatgcc agagaagtat acttacgaat agaggcagga aatgaaaaaa
     5941 gtgaacaacc ttcattgcct tttattgcta tgctcaataa ttttcggata tggaaaagta
     6001 cgatcaaaga aaaaaggagt gacaaacgat ggatatcata acaaaatttg ctgagggatt
     6061 tatgaggctg ttccaaacag gtgcagaaac attcataagc tggatgacag gaatcgtacc
     6121 agttgtcttg atgctgatgg ttgcaatgaa tacgctgatt gcttttttag gtgaagaccg
     6181 agtaacgaaa gtagccaaat tatcagcgaa aaatccgctt actcgttacc ttgtgttacc
     6241 gttcttatct gcttttatgt tgggaaatcc tatgtcattt acaatggctc gtttccttcc
     6301 agaatactac aaaccaagct attatgctgc tcaagctcaa ttttgtcata cgagcaatgg
     6361 tgtattcccc catattaatc ctggagaatt gtttgtatgg atggggattg ctcaagggat
     6421 cgaaacctta gggctaaatt cgatggagct ggctatccga tacttgcttg tgggactgct
     6481 gatgaacttt attggtggtt ggataactga ttttactact ggatttgtat gcagacagca
     6541 aggaatcgtt cttagcaaaa aagtagagct ttcagttgat tagagaaagg agagttttaa
     6601 atgtatggtg atattaagat cgtaagagga aacggcgggt atggaggtcc tttgattatt
     6661 aggccgacaa aagataaaca caagttcatc tatgtcacag gtggaggtga gaggccaatg
     6721 atcgtagata aaatagaaga actgacaggt atggagtcta tcaatgggtt caaaacatca
     6781 atacctgata gtgaaatcgc gcttgcaatc gttgactgtg gagggactct tagatgtgga
     6841 atttatccta aaaaagggat accaaccatc aatatcgttc ccacaggaaa aagtggccca
     6901 ttagcacaat atatcactga agatatttat gtttcagcag tagggttaga acaaatacag
     6961 ccactaatgg aaactgatga taacaaagaa acagtaaata ctgcatctcc agataaaaag
     7021 tacaaatatg atacttctaa aaagataacc gagcaaaaag cagagcagca aaactggata
     7081 gcaaaaattg gaatgggcgc gggaaaagta gttgctacat tcaatcaatc agcaagagaa
     7141 gcagtagata cgatgatccg gacagtgatc ccttttatgg ctttcgtttc ccttttgatt
     7201 ggtgtgatac aaggttctgg gatcggtaat gtatttgcta acattatggc acctttagca
     7261 ggaaatgcag ttggattaac aatcattggc tttatttgtt cactgccatt tttatctcct
     7321 ttgcttggtc caggggcagt tattagccaa gtgattggaa cattgatcgg tgtagagatt
     7381 gggaaaggaa atattcctcc ccaattagca ttaccggcac tatttgctat caatactcaa
     7441 aatgcatgtg attttattcc agtcggtcta ggtttggaag aagcaaaagc tgagacagta
     7501 gaagtaggtg taccatcagt tctttattca agatttctta acggcatccc acgagttatc
     7561 attgcttggg tagcaagttt tggattatat agctga
//
LOCUS       DACEMS010000115         7453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEMS010000115 DACEMS010000000
VERSION     DACEMS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.120"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..2113
                     /locus_tag="IW735_002279"
                     /pseudo
     CDS             100..2113
                     /locus_tag="IW735_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            2239..2568
                     /locus_tag="IW735_002280"
     CDS             2239..2568
                     /locus_tag="IW735_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1045256.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2608..3612
                     /locus_tag="IW735_002281"
     CDS             2608..3612
                     /locus_tag="IW735_002281"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1045257.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3609..5038
                     /locus_tag="IW735_002282"
                     /pseudo
     CDS             3609..5038
                     /locus_tag="IW735_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5244..6416
                     /locus_tag="IW735_002283"
     CDS             5244..6416
                     /locus_tag="IW735_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1045258.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6578..7033)
                     /locus_tag="IW735_002284"
     CDS             complement(6578..7033)
                     /locus_tag="IW735_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045259.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         2531 a         1384 c         1474 g         2064 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gaatcttact tacggaagtg ctgcacaaga
      961 cctgacgact aatacgccta ctatcaacgg atataccttt acagctgttt cagcgacaga
     1021 aacaaagaac caatcagcaa tgagtgtttt agctagttta gatacaaatg gaaatgtaat
     1081 cgtaaaggac gcaaatggaa aacagatttc tgaagtcatc ctatattata agacaaatgc
     1141 aacagtatca atcaatgcaa atggcagtaa gtactatgat ggtctttctg ttgtaccgat
     1201 cgttaaatat gcctttaatg ataaaacaga atcaacatcc cttttaaaca atgatctcag
     1261 tattgatcaa atcacatgga atccaactga tttcaaagcg atgaatgaaa agggagttga
     1321 agtaactgcg cctactgaaa ttggaaccta tacgtcttgg caattgacaa aaagcggact
     1381 agctaaacta gctgccagaa caaactattt atttactatc gtgcagacat ctgatgtcta
     1441 tacgattaag caaatcagcg gtgcggtaga gctcagtggc agtaagacat atgatggaaa
     1501 agcaggtgtc ccgtcaatcc atgtcaagct agcagagggt gtcactagtt cgcttactcc
     1561 agtacaagtg gcacttagca gtatggatta cacagcagat gcacaatcag caaaaaatat
     1621 ggttaatgct ggcagctata caattaaact aacgaatagc ggaatagaca aagtcaaagc
     1681 agcaaatccc aagttgtcat ttactgatat agccaacaca tcaggaactt ataccatcta
     1741 taaagctaac gcagtgatta ctgtcgatga cgctgccttt aattatgatg cccaaagtca
     1801 ctcgatttcg gttggaaatc tccacgttac cggtgttgtc ttaggcgaat cattggatta
     1861 taccctaaca gttaacagcc ggacagatat tggatcacaa atagtaacga ttagtttagg
     1921 cgaaggaagt gtaaacaata attatacgat tatcgcaaca gataccgcta agttgacgat
     1981 caatccgact ctcttgcttc cttcgacagg tgggataggt ctactgccct ttgtcttttt
     2041 aggactgatc tttatttttt ctggcttctt ttattttatc catcgtaaga aaaaagcagg
     2101 tgaacagcga tgaaaaaaac aaaaaatgta cgtgtggata tatttgctgt tctctttttt
     2161 atcctactgg tcttttttat aggttctccg actgtgtcag ctagagaatc acctattaca
     2221 tttactttgc ataatcgaat gttcgaaagc gataagatgc caagtatcag tcaaaacgac
     2281 ggaataaata tcccggataa ctttcaagga gtaaaagggt tgaacggcgt tactttcgct
     2341 atatatgatg tctcggatga attttacaag ctgcggtcag aaggaagtag tgtggaagac
     2401 gcacaaagaa aactagcaca aaaaagtgat agtgaaaaaa tactggcaga aaatgtgaca
     2461 aaaacagtag acgaggaaga gggcattgct tcttttagtg catcggacaa agatgagcaa
     2521 ggtcgtgacg ctgtttaccg atttgcggaa ataaagactt cagattaagt aaaagaaaaa
     2581 tcagcaccat ttgtcgttgt gcttcctgtg acagattcat ctaatcataa attgacgacc
     2641 atccacttgt atccaaagag cgaacaaaaa atacctgctg cactcacatt aacaaaaaca
     2701 gtagagaata agcagacaga ttttgcagat ggggataaag ttccttatct catcaccgta
     2761 acaattcctg agaacatcaa cgagatagga acctatacga tcaaagatac agctgatcct
     2821 cagttatggc tagaaccaga aacgatcgat tttttgattg gtggagacaa gattcataca
     2881 tttcacacac aaaaaacaaa acatggtttt gtcttatcag attctggaaa agatcttagt
     2941 tcgtttgctg ggaaaaaatt aacgatccgt taccaaatga cattgaaaga aaacagtgaa
     3001 aaaacgacat ttgataacga tgtacgtttg acgactgaca atcaaacagt agaaacacat
     3061 cttgccatcc aaacaggagg caaacaattt gtcaaagtgg atcggcaaac gaaacaaaaa
     3121 ctagcagatg ctcaatttct agtcaaaaaa aatgaccaat acttagcgca agaaaacggg
     3181 ataaatcatt gggtagccaa agatgattca agagcaacga tttttacttc cagtaaagat
     3241 ggaacatttt ctgtccatgg attgtctttt ggaagttacg aactagtgga gattaaaccg
     3301 cctaaaggat atatattgag tcaatcaacc attctctttg aagttgaaaa aggcagttat
     3361 gatccgcttc atcctatacc attggaaatc atcaacgaac caaaagcaaa tccgacgaat
     3421 aatggaaaaa cgccagttca gatgcagaca aatggtccat caaatgaaaa aaatggagga
     3481 agctttccaa aaacaagcga agagatgctt ggaggtttct caattttagg gttgttgcta
     3541 gtcctttcaa cagggacagc ttggttttat aaaaaacaac aaaaaggaaa caataggagg
     3601 gaaataaaat gaaaaacatc aaaacaaaaa tatggacgtt tttaggaact gctatcatgc
     3661 tgcttccttt cgttctcggc ctaggaactg cagaggtttc taggccgtat cacctacacc
     3721 agaaaatgtt acggttaatt tgcataaatt gaaattcact tcagcaccag aaaatcaaat
     3781 aaacaacgga acggaattga catttccgaa tagcgaaccg ttgaacggcg ttgaattcaa
     3841 cgtatatgac atcactgcta cgtactatcc aagtaaagac acagcagttc cagcagacgc
     3901 tacacccttc gcaagtgtaa cgacttctgg tgaaggatta gctaacctga ctcttccagg
     3961 taaatctgat gggaaagatg ctgtatacgt ctttgtagaa acaccaaagc ccggtgtcga
     4021 aacgtcgcca aatatcgtgt taagcttacc cttttataac ccgaattcat cagaccctga
     4081 caaaccttta gacacgattc acctatatgc taaaaatgtc atcaaagaaa gcaatatcaa
     4141 aatccagaaa gctgggaact tttcaggggt agataagtta gcaggggcgc aattccagat
     4201 caaaaattca gagaatcaat tcgtgaccgg ttttgatgaa aacactggtg tcgctctcta
     4261 tgatcctgct gagtcagcgt tgacattcac aactgatact gaaggatcca tcagtgtcaa
     4321 tggattaata gacggcaatt atactttagt tgaaacaaaa gcaccagatg gttatcaatt
     4381 aaccaatcca caaaaagaaa ctacttttat agtaaaaaac ggaaaattgc aacaaacaac
     4441 cggtatcatc catggcgcag atgaagccaa tcttgggaat ccaaacggtg tagtcaatcg
     4501 ggaaaataca actacggata acacgattac tgtagaaaat gtccaaaact acggtgattt
     4561 ccacttcatc aaacaagatg tgaacacgca agaaaaatta gccgatgcag aattttctat
     4621 tctaagagca aaagatccaa ctgttaaatt aatgaaaaaa atagcaaatc ttgcctcagg
     4681 agaataccca tatgcttggt cagatgaagt cacaggttct gactgggaag aagtgaaatt
     4741 gatttctgat tcagaaggga aattttcaat cgaagggctg aaacaagggg catactgcct
     4801 tcaagaaaca aaagcaccag aaggctacgc attacctaag aatgaagctg cgtacactga
     4861 atttacagta gatgatgatc caacaacttc tgatgaaaca acgatcgata accaacctaa
     4921 aggaatcttg ccttcaactg gtggtatggg gatcatcgta ttcatcttgg taggtactgc
     4981 tttagttgga ggagcagtga tctacttcaa aaaacgccat caagaaacag aagcttaaac
     5041 tattggaaac aaaaatcata cgataaataa acgatagtga ttcaaatggg gagaaaggtt
     5101 gtgacaagtg gtcggtctct tggtattcgc tcaacaaacg gcaaaatagg aggaactatt
     5161 ttgccgtttg ttgagcgaat actcaggtca aggcgctgat ttcacaacct cttcatatac
     5221 atattagaaa ataggtggac atgatgaata aaagcagaat aagaatactg gacatcttca
     5281 tggcaatcat tttagtcgtt gggatcggga tcttttctta tccttttgta gaagattcac
     5341 tgaatgattt tcttgcccag caaatgatca tccattatca aaagcaagca tcgaataaga
     5401 attcagagga aataaaaaaa cagcaggaaa aaatgacaaa gaaaaaccaa cagttggcag
     5461 agcaaaacgt atcaccaggg ataggcagtt tcaaccaagc agttgatgca aaagcattaa
     5521 aagatttacc tagtaatgcc ttttttatgg aacatatgct tggagtcatc gaaatcccaa
     5581 aaatcaatgt cagcttacct atattcgatc agacaacgga aatatttttg caaaaaggaa
     5641 cgtcattatt ggaagaaagt tcttatccga ctggcgggaa aaacacccat gccgttcttt
     5701 ctgggcatcg tggactccca gaagcaaaat tatttacaga tttgcctaag ttgaaaaaag
     5761 gagaccagtt tttcatccag atcaatggaa agacattggc gtatcaagta gaaaaaatcc
     5821 aagtggtctt acctgatgaa gtagattctt tgggtatcca aaaaggcaga gatctcgtga
     5881 ctttgcttac ctgtactcct tatatggtca atacacatcg actgcttgtg acggggcatc
     5941 gtattcctta tcaagcaaaa gaggcaaaaa aagcgatcca aggaattgat caatggaaaa
     6001 aatggaaatt ttttatttgg tttatcggta tactacttgg atcaatcggc ttagtttggc
     6061 tactcatcgc ttatttagat tctttggcca tcgctaagcg aaactatcct ttatcttttt
     6121 atgtaaagaa cacaaacgga cgaccaatcg aaggaatggt cttttctgta aaaacgctca
     6181 acggcaaaca ctatattact agagaaaaag ttccatttgt gaaagcttct gatgaatacg
     6241 gactagtgag attttctgat ttaaaaggca ggaactatcg cttgcaacat gaggagctcc
     6301 tgcttaaaat ccatgtaaaa cataaacatt cgaaacagtt ttcaatgaaa ttgaaaaaag
     6361 gaagatataa actgcgaaaa gaaaaagagg tgtactattt gattgagaaa gaatgaatga
     6421 agatgtccaa aaaagcagag ggaacagact aaatgaaaaa agagagaagc tgacgaaatg
     6481 gacgtgtaaa ctgctatttt tggtttacta tctggaacgc cagcttctat ttttttatgt
     6541 ttcagaagaa ttttattatt atagtataac ttttgaatta gtttccataa catttggagc
     6601 aaggggttaa accacgagct tgagcttcgc ttaaagtggc aggggtatac gtaccatttc
     6661 cacatttatg tgtatggtat tttgaaccgg tcggtgtgac atagacagtt tgttcattat
     6721 tttgctcagg agctgcggct tgtgcctgct gagaggcctg ttcagctgct gcttgctgtc
     6781 gcgcctgttc tgcttgttgc tgtgcgattg cttcttgttg cgcttgttct gcttgttgtc
     6841 gtgcaatttc ttcttgcttt ttccgttctt cttctgcttc ttttgctttg attgtggaat
     6901 caattgcagc aagacggctt gtcaactctt gtttgttttc aggtagcgct tggatagctg
     6961 ctgaagcagt tgcatagttt tcttttgtag gatttgcttc agcttgtgac aaagcagatt
     7021 ccgcttctgt cacttttttt tctagtgcag ctttttccgc tttttgtttc tcttcttctt
     7081 ttttcttttc aatttgttct tgttctttcg atgaatctgc cgtactttcg gagatcgtgg
     7141 tactgctgct ggaagcctga gctgaacttg tgctttgagt agtagacgta gtagcaccac
     7201 acccactcaa caaacttcct gcgaccaaaa acaatgcaac taattttttc actaaatttc
     7261 ctccttaagt aaaaaacaac cgttttcaat aaatgtcctt ttcaattgtt tacttacttg
     7321 ttataataat tgtgttatga atagtgattg aggaaactta atctaatccc gttttgatac
     7381 acgggatttt ttgctatagt tctgttatta agtatgcaat aataaaatat gtacgttatt
     7441 ttattattag att
//
LOCUS       DACEMS010000116         7400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEMS010000116 DACEMS010000000
VERSION     DACEMS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.121"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..151
                     /locus_tag="IW735_002285"
     CDS             68..151
                     /locus_tag="IW735_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1045260.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="IW735_002286"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="IW735_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1045261.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="IW735_002287"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="IW735_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1606..2271
                     /locus_tag="IW735_002288"
                     /pseudo
     CDS             1606..2271
                     /locus_tag="IW735_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2307..2552
                     /locus_tag="IW735_002289"
     CDS             2307..2552
                     /locus_tag="IW735_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1045262.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2655..3524
                     /locus_tag="IW735_002290"
     CDS             2655..3524
                     /locus_tag="IW735_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1045263.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3505..4239
                     /locus_tag="IW735_002291"
     CDS             3505..4239
                     /locus_tag="IW735_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1045264.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4272..5184
                     /gene="ant(6)"
                     /locus_tag="IW735_002292"
                     /pseudo
     CDS             4272..5184
                     /gene="ant(6)"
                     /locus_tag="IW735_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5181..5723
                     /gene="sat4"
                     /locus_tag="IW735_002293"
     CDS             5181..5723
                     /gene="sat4"
                     /locus_tag="IW735_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1045265.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5816..6610
                     /locus_tag="IW735_002294"
                     /note="aph(3')-IIIa"
     CDS             5816..6610
                     /locus_tag="IW735_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1045266.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6718..6906)
                     /locus_tag="IW735_002295"
                     /pseudo
     CDS             complement(6718..6906)
                     /locus_tag="IW735_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6886..>7400
                     /locus_tag="IW735_002296"
     CDS             6886..>7400
                     /locus_tag="IW735_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1045267.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2593 a         1119 c         1555 g         2133 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1501 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1561 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1621 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1681 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     1741 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     1801 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     1861 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     1921 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     1981 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2041 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2101 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2161 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2221 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2281 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2341 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2401 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2461 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2521 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2581 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2641 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     2701 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     2761 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     2821 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     2881 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     2941 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3001 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3061 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3121 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3181 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3241 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3301 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3361 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3421 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     3481 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     3541 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     3601 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3661 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3721 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3781 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3841 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3901 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3961 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     4021 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     4081 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     4141 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     4201 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     4261 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     4321 aggatgaacg aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata
     4381 cctaaagatg aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt
     4441 atatctaatg atgactggct taatcaattt gggaatataa taatgatgca aaagccggag
     4501 gatatggaat tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat
     4561 tacaataaaa ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc
     4621 gataaattaa taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg
     4681 actgatatag attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat
     4741 gaattttgga atgtaacacc ttatgttatt aaaggattgt gccgtaagga aattttattt
     4801 gctattgatc attttaatca gattgttcgc catgagctgc tgagaatgat atcatggaag
     4861 gtcggcatcg aaacaggctt taaattaagt gtaggcaaga actataagtt tattgaaagg
     4921 tatatatccg aggatttgtg ggagaaactt ttgtccacct accggatgga ttcctatgaa
     4981 aacatatggg aagcattatt tctatgccat caattgttca gggcggtatc cggtgaggtg
     5041 gcggaaaggc ttcattatgc ctatccggag tatgatagga atataacaaa atataccagg
     5101 gacatgtata aaaaatacac tggtaaaacc ggctgcctgg atagcacata tgccgctgat
     5161 atagaagaga ggcgggaaca gtgattacag aaatgaaagc agggcacctg aaagatatcg
     5221 ataaacccag cgaaccattt gaggtgatag gtaagattat accgaggtat gaaaacgaga
     5281 attggacctt tacagaatta ctctatgaag cgccatattt aaaaagctac caagacgaag
     5341 aggatgaaga ggatgaggag gcagattgcc ttgaatatat tgacaatact gataagataa
     5401 tatatcttta ctaccaagac gataaatgcg tcggaaaagt taaactgcga aaaaattgga
     5461 accggtacgc ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag
     5521 gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa
     5581 tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca
     5641 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     5701 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     5761 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     5821 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     5881 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     5941 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     6001 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     6061 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     6121 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     6181 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     6241 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     6301 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     6361 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     6421 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     6481 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     6541 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     6601 attgttttag tacctagatt tagatgtcta aaaagcttta actacaagct ttttagacat
     6661 ctaatctttt ctgaagtaca tccgcaactg tccatactct gatgttttat atcttttcta
     6721 aaagttcgct agatagagtt ctatattaga gatgtaaaga gttttggtga agaggtttgt
     6781 gagtatggct tttataacca gggtgcaatg agaagcacta acagcagcta gcttaagaac
     6841 gcataggata tactttttat ggaagtcaca gaggaggaat aaaatttgaa caggattaac
     6901 agagtaacct ctattcttat tcagctgcaa tcaaaaaaaa taattcctgc caaagaaatt
     6961 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     7021 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     7081 cttccgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     7141 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     7201 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     7261 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     7321 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     7381 ccggaaagca gaatgataga
//
LOCUS       DACEMS010000117         7344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEMS010000117 DACEMS010000000
VERSION     DACEMS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.122"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            140..460
                     /locus_tag="IW735_002297"
     CDS             140..460
                     /locus_tag="IW735_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045268.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IW735_002298"
     CDS             912..1112
                     /locus_tag="IW735_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1045269.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IW735_002299"
     CDS             1259..4048
                     /locus_tag="IW735_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1045270.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IW735_002300"
     CDS             4095..4622
                     /locus_tag="IW735_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1045271.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IW735_002301"
     CDS             4643..5833
                     /locus_tag="IW735_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1045272.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IW735_002302"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IW735_002302"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1045273.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2439 a         1340 c         1576 g         1989 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtg
//
LOCUS       DACEMS010000118         7187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEMS010000118 DACEMS010000000
VERSION     DACEMS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.123"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW735_002303"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW735_002303"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1045274.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IW735_002304"
     CDS             complement(1172..1867)
                     /locus_tag="IW735_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1045275.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IW735_002305"
     CDS             complement(1880..2596)
                     /locus_tag="IW735_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1045276.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="IW735_002306"
     CDS             2926..3534
                     /locus_tag="IW735_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1045277.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IW735_002307"
     CDS             3859..4182
                     /locus_tag="IW735_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1045278.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IW735_002308"
     CDS             4364..5734
                     /locus_tag="IW735_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1045279.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="IW735_002309"
     CDS             6081..>7187
                     /locus_tag="IW735_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1045280.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DACEMS010000119         6866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEMS010000119 DACEMS010000000
VERSION     DACEMS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.124"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(310..807)
                     /locus_tag="IW735_002310"
     CDS             complement(310..807)
                     /locus_tag="IW735_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1045281.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IW735_002311"
     CDS             complement(847..1656)
                     /locus_tag="IW735_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1045282.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IW735_002312"
     CDS             1990..2847
                     /locus_tag="IW735_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1045283.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IW735_002313"
     CDS             2840..3742
                     /locus_tag="IW735_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1045284.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="IW735_002314"
     CDS             3775..4125
                     /locus_tag="IW735_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1045285.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="IW735_002315"
     CDS             4159..5097
                     /locus_tag="IW735_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1045286.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="IW735_002316"
     CDS             5175..6068
                     /locus_tag="IW735_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1045287.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="IW735_002317"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="IW735_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1045288.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..>6866
                     /locus_tag="IW735_002318"
     CDS             6683..>6866
                     /locus_tag="IW735_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1045289.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFK"
BASE COUNT         2317 a         1200 c         1322 g         2027 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaat
//
LOCUS       DACEMS010000120         6807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEMS010000120 DACEMS010000000
VERSION     DACEMS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.125"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(362..1480)
                     /locus_tag="IW735_002319"
     CDS             complement(362..1480)
                     /locus_tag="IW735_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045290.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1951..3258
                     /locus_tag="IW735_002320"
     CDS             1951..3258
                     /locus_tag="IW735_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1045291.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="IW735_002321"
     CDS             3377..4576
                     /locus_tag="IW735_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1045292.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="IW735_002322"
     CDS             complement(4603..5871)
                     /locus_tag="IW735_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1045293.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6034..6680)
                     /locus_tag="IW735_002323"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="IW735_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
BASE COUNT         2055 a         1427 c         1210 g         2115 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6721 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcccggac tcttttaaat
     6781 aagatacgtt ttgcatctta tcatcac
//
LOCUS       DACEMS010000121         6610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEMS010000121 DACEMS010000000
VERSION     DACEMS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.126"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            810..1484
                     /locus_tag="IW735_002324"
     CDS             810..1484
                     /locus_tag="IW735_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1045294.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1481..1822
                     /locus_tag="IW735_002325"
     CDS             1481..1822
                     /locus_tag="IW735_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045295.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            1852..2211
                     /locus_tag="IW735_002326"
     CDS             1852..2211
                     /locus_tag="IW735_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1045296.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2414..2593
                     /locus_tag="IW735_002327"
                     /pseudo
     CDS             2414..2593
                     /locus_tag="IW735_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            2735..4816
                     /gene="cadA"
                     /locus_tag="IW735_002328"
     CDS             2735..4816
                     /gene="cadA"
                     /locus_tag="IW735_002328"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1045297.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            5127..6593
                     /locus_tag="IW735_002329"
     CDS             5127..6593
                     /locus_tag="IW735_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1045298.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2188 a         1075 c         1410 g         1937 t
ORIGIN      
        1 ggaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag
       61 tttttcatgt ttgatgacta ttgtgacttg tgaccgagga gttggccgga gagcaccgtt
      121 tattctaaaa aagaactgcg aactctcgca gttctttttt taaaatacgg gataaaggaa
      181 gtttccagca gacaagagaa gaaagcaaaa gaaattgcta tttgattcac aaggttctat
      241 aacgaaatcg caatcaatca aacatttaca aagaaggtaa ttcaaatgga aatctgattt
      301 gatgatgctt ctgctgttga aataaagcag catctagcac ctgaaaacaa actaatagta
      361 acattcgaag atggagtagg tccgtatttt cagcatgcaa taatccatat gtaagttcaa
      421 ttcacccttg aaatcatcgg accagataaa gttatgacca atctatccca agcaatctag
      481 aagagatatt agtaaaggga catttactag aagtcttaga tgagcctata aatatatgtt
      541 ctgatggcgt catcagtggt ctgattgacg gaagcttagg tgtgatcgat ctctctggac
      601 caaacaacga aggaatcaag gggaatccgg cacgttaaga aaaaaatagc ataaaaaact
      661 agacacttga tgagcgttca ttcaattgtt tgaagggact aggctggtgt cattttttat
      721 acatatattt tctctttcgt cttgataggg cttatgggat aatataagga agaaaatcat
      781 cgttttattc aaaaaggagt ttttgacaaa tgaaaaaacg tatattattt atcggtgata
      841 gcattacaga cgcaggaaga gaccgagcag aagcaactga tctaggtaaa ggttatccac
      901 ttttgattgc cggaaaacta atctcccaat atccaacgat ggaatttgaa tgcatgaacc
      961 gtgggatagg aggagacaaa attgcagatc tgcaagtacg ctggcaagaa gattgtttag
     1021 ctttacaacc tgactatgta tcgattttga tcggaatcaa tgatacctgg cataatacta
     1081 gcgatcaggc tatattcgga actgagaaat ctgctcagca atttgaatcc gtttatcgaa
     1141 aattactaaa tgaacttcaa acagtgggaa tctgcaacat catcttgatg gagccgtttg
     1201 tacttcctat aaccgacgac cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa
     1261 ttgttcgtaa acttgctaag gaatatggcg cattgctggt tcctttagat ggtatattca
     1321 atgctttagg gattgtttat ggtttttcga aatatacaag agaagatggg gtccacccaa
     1381 cgattggcgg acatgagatc attgcacaaa gctggataga tgcaattgcc gcagtcgatt
     1441 tttttagtgg agataaaaat gagcgaaaga aaagtgagaa atgatggaaa tacggtgggc
     1501 agtaaaagaa gatctacctc aaatcaatca gctgattact gatcatccag aatcattcgt
     1561 aaaagcaaaa acggcagctg acctaaatga taagaataaa ctattgctcg ttgctgtaac
     1621 aaaagaaaaa cggattatcg gatttgcgca agcggacgtg caacagtcag aagaacgatt
     1681 ggatcagcga ccatcaggaa aactaatcca acacacatta gtaacaaaag aaaatacgga
     1741 agaaatccaa aaaaaatttg cagaatattt gatactggaa gctaaaaaaa gaagaataaa
     1801 atcatttttt gcccagtttt aatcaaaaac agtaatagaa ggagaaatac aatgagttgt
     1861 ttaggaaatg tcatctggtt tatattcggc ggactaatcg gtggtcttag ctggttagca
     1921 gcggggatac tttggtgtat cacgatcata ggtatcccaa tcggtatgca atgttttaag
     1981 atcgcctccc tttcttttgc gccttttggc aaagaagtaa tttatcacca gtctggcatg
     2041 tcattgattg ttaatatcat ctggctgatc gtttcaggcc taccaattgc tctcgcacat
     2101 gtcgtcagcg gcatccttct atgtatcacg atcataggga taccttttgc caagcaatct
     2161 ttcaaacttg ctcgattggc gttgatgcca ttcggtgcag aaattgtata aaagagaata
     2221 ataaaggtta gataaagcat agagtcaggt gggatacatg tatttcacct gactctatgc
     2281 gttctcctgt ataagttctt ccatgtgact tttacgtctt ttatttatat ggggttactg
     2341 tttatggaaa gttgaaataa ttttcattaa agaactcttt ctatattttt gaaagcgtag
     2401 tggagcttcg aatatctggg aaggacattt tgcagggatt gaaaaaagat attgtcctat
     2461 cgaaaaatta atgaatttag acaaattgcc agcaacaggc ttcaaactta gttgctatcc
     2521 tgtcaagatc aagaaggcaa gtgcgggttg gataagagca gtggcaatga tcgaaaagaa
     2581 aaagaaagaa taagccaaaa catctgtatg gaagaaattt tccgatgcag atgtttttca
     2641 ttttttcttt cggatacctt gacatatgaa taaccgttca tttataatta gttatgaaga
     2701 gatattcata tgttgaggcg aggaggtcat taaaatgaaa agttacagat tagaaggttt
     2761 agattgtgcg aactgtgcaa tgaaaatcga aaaaggagtt cagaagatca atggagtcaa
     2821 ggaagcaacg gtaaacttca cttctggaaa actaacgatt gacgcagagg aagatcattt
     2881 ggctacgatt gaacaagaaa taaaaaaagt agtgaaggaa ttagaaccag atgtcaaagt
     2941 aacagaaata gataaggaaa aagtatctga acatggaaat gaaaaagaga gaaatacgct
     3001 gttccggatt ttattttccc ttgcaggtat cgctttattg ctattatttg attttaatga
     3061 accaattcgc ttgatagggt atttattgat ttatctgctg ataggatatg atgtagtgaa
     3121 gaaggcagtg atgaatatcg tcaaaggaaa aatctttgat gagaattttc ttatgtctgt
     3181 tgctacgatt ggcgctatgt tgatcggtga atatccagaa gccgtagcag tgatgctatt
     3241 ttatcaaatc ggcgagtatt tccaaggatt ggcagtcagt cattcaagaa aatcgattcg
     3301 cgaattgatg gcgatccgtc cagaaacagc tcacgtgcag acgacggaag gattgatgac
     3361 cgtgaaccct gaagacgtgc agatcggtca gtttgtactt gtgaagcctg gtgaacgtgt
     3421 accgttagat ggaacgatca tcgaaggaga atcattagtc gatacgtcag cacttacagg
     3481 agaatcagtc cctaaatctg tatatgttgg tgaaacagta ttaagtggat ttatcaataa
     3541 aaataaacca tttttagttc aagtagaaaa atcttatgaa aattctacta tcagcaaatt
     3601 attagaatta gtagaaaatg ccagcagtaa aaaagcacca gctgaaaatt tcattactaa
     3661 attcgcgcgc tattacacac cagtcgttgt aggattggcc gttttattag ctgttttacc
     3721 tccacttgtt gtttctggag cggcgttttc tgaatggatc tatcgagcat tgacattttt
     3781 agtgatctca tgtccatgtg cattagtgat ctccgtaccc cttagtttct ttggcggtat
     3841 cggaggagca agtaaaatcg gtgtattagt aaaaggttca aattacttgg agttgcttgc
     3901 acaaactgag acagtggtat tcgataaaac cggcacatta acgaaaggtg acttttcgat
     3961 ccagacgatc gatacaaaaa tcgatccaaa gatattcatg caatatgttg cgagtgcaga
     4021 acagttttct acacatccga ttgctcaatc tgttttagaa ggctacgcag gtccgctttt
     4081 accaacagct aatatccaag aatttgcagg tgaagggatc ttagctgaag ttgatggcaa
     4141 acaagtatta gcaggtaatc acaaattgat ggaaagattt gaaatctctt ttccttcaag
     4201 tcaagaaatc ggtacattgc tttatctagc gatcgatcaa agttacagtg gttatcttgt
     4261 tattgcagat accttgaaag aagatgccgt agacgcattg gttcaattaa aaaaggctgg
     4321 tgtgaagaac acagtcatgt tgacgggcga ttcgaaaaaa attgctgatc atatcgggaa
     4381 gcaagtaggc gtggacaaga tatatagcga attgcttcca gaagataaag tgcagcggtt
     4441 agaggaaatc ttacaaagaa acaataaaaa gaccgcattt gtcggagatg gaatcaacga
     4501 tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg ggtggattgg gaagcgatgc
     4561 tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa ccatcgaaga ttgcagaagc
     4621 aatccattta gcaaagaaaa cattgaaaat cgtcaaacag aacattgtct ttgctattgg
     4681 gataaaaatt ttggtacttg ccttaggtgc ttttggtttt gcatcaatgg gagatgcagt
     4741 atttgcagat gttggggtga cagtattagc tgttttgaat gccatgcgaa gcttgcatgt
     4801 aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt cctctttttt ttatatcctt
     4861 ttcttaatga aaaatgtaaa gaaaatagat aggattataa aataactaat gtttttaatt
     4921 ttgcagaaaa ttcaaaatag cttgaaattc aataaggtaa atgaaataaa agaaaataac
     4981 ttattttttt gatggaaaaa atatgaaaaa acgatgtgta ttaaataaaa gaatacacac
     5041 ataaaataac actgtcttgt aagtttgtta aaaattgaat atgattacat ctggataaca
     5101 aatttttatc ataaaggaga gaacgaatga acaacaaaca attacgctgg tttactgttg
     5161 cgttaattgc atttaacatg gtttggggca tggggaatgt agttaataac tatgctcagc
     5221 aaggagtatc agttgtcacc tcttggctat tgattttggc catttatttt attccatacg
     5281 cattgatcgt tggacaatta gggtcagcct tcaaggatag taaaggaggc gtcagttcat
     5341 gggtcgaaaa tacgacatct aataaacgac tggcatacta tgccgcttgg acttactggg
     5401 tcgtccatat tccttatttg gcacaaaagc ctcaagcaat cctgattgct ggcggttggg
     5461 cagtccaagg aaatggaaat atcgtaaata cgatgtcagt acaaatggta gcagttattt
     5521 cacttattat atttcttgcc tttttatatt tatcaacaaa aggactttct actttgaaag
     5581 tgattggcgg actggccgga acagcgatgt tcgtcatgtc gatcttattt attcttttag
     5641 ctgtaacggc accgttcatc aatccaacga tggaatttgc cacaccacat atgaatcaag
     5701 tgaaaacata tattccaaca tttgatttca gttatttcac aaccgtttcg atgctagtat
     5761 ttgcagttgg aggagcggaa aaaatatccc cttatgtcaa ctctacaaga aatcctgcaa
     5821 aggaatttcc taaagggatg atcttcttag cagcaatggt agggatttgt gctgtattag
     5881 gctcagtcgc aatgggtatg ctgtttgcta gcgataatat tcctaaagac ttgatggcaa
     5941 atggggcata cagtgctttt caaattttag gtaattacta tggtatcggt aactcattga
     6001 tgatcattta cgcattgaca aacatgattg gacaaatctc cgcattagcc ttttcaattg
     6061 atgcaccact aaaaatcttg ttagcagatg cggatccaga atttgtacca aactggctac
     6121 gtaaacgtaa tcgtaaaggt acattggtga atggttatct tctgacaggt atactagtag
     6181 gaatcattat tttgttgccg atctttggta tcaaagatat gaatgaatta gtaaaatggt
     6241 tgacgaactt gaactcagtt gtcatgccaa tgcgttacct atgggtgttc ttggcatata
     6301 tgatgttgaa taaagcttat aagaaattca attctgaata taaatttgtt aaaaatccaa
     6361 aaatcggttt tatctttggt gcttggtgct ttgccttcac tgcgtttgca tgtatcctag
     6421 gaatggtgcc aaaagtagat tatactgctg atcctaaagc atggtggttc caattattca
     6481 cgaatatcat cacaccaatt gtcttgatct tattaggtat gatcttgcca gcaattgctc
     6541 gtcgagacaa aaacaaagaa atcactgaac cagaaacagt ggttcagcct taaaatagaa
     6601 atacttgtta
//
LOCUS       DACEMS010000122         6601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEMS010000122 DACEMS010000000
VERSION     DACEMS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.127"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..1269)
                     /locus_tag="IW735_002330"
     CDS             complement(43..1269)
                     /locus_tag="IW735_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1045299.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(2023..2223)
                     /locus_tag="IW735_002331"
     CDS             complement(2023..2223)
                     /locus_tag="IW735_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1045300.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(2357..2965)
                     /locus_tag="IW735_002332"
     CDS             complement(2357..2965)
                     /locus_tag="IW735_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1045301.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(3050..3520)
                     /locus_tag="IW735_002333"
     CDS             complement(3050..3520)
                     /locus_tag="IW735_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1045302.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(3568..4482)
                     /locus_tag="IW735_002334"
     CDS             complement(3568..4482)
                     /locus_tag="IW735_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1045303.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            4628..5431
                     /locus_tag="IW735_002335"
     CDS             4628..5431
                     /locus_tag="IW735_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1045304.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            5609..6556
                     /locus_tag="IW735_002336"
     CDS             5609..6556
                     /locus_tag="IW735_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1045305.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         2062 a         1318 c          998 g         2223 t
ORIGIN      
        1 tctaatttag gtggcactct ttattaacca aactattttt ttctagtacc cacttgttgt
       61 tccatactca ttattctggt cataaccata accattttgt tcctgtccag cgtaaggatc
      121 ataactttga gtatcttgac ttggtgtata gtactgactt gtatcttctg tttgctgata
      181 ataggtattc ggatcttggt actgatcata tgtgttttct gaataggaag aataatcggt
      241 gtacccttca ctattcgatc catcaccgtt tgtcgaatcg ccatcttccg aatcatcaaa
      301 gaacatcatt gaagcattct catttttcaa attaggtaat gtatcgcttg tttttatatt
      361 taattgtttc ttcagttcat tttggatcga aagaagatca ttttttccta aaatttgata
      421 gtagatatca ttgatcattt gtccttcgcc ttgcaactgg tcttgtttga ttgtttcaaa
      481 cgcaggggtg tagttcgtcg ctacatcaag catgtcatcc caatctaagt ccgtcgtcac
      541 attgttctca actgctttta aaatttttct ataattgctt acgccatcta aacttaaaac
      601 tttattgacg attttcgtca ctacttcccg ctgacgacgt tgtcgaccga tatcgccttc
      661 tggatcttcg tgtcgcattc gagaataggc taaacctttt tctccattca atgtttgttc
      721 acctactggt acatggacac catccaaagt aaagtcaatt ttattattca ctttgatgcc
      781 accgaccgca ttgatcaaat cttccatgcc atccaaattg atcgttacat aatgatcaat
      841 aggaatatcg agcagttgct caattgaatc catggccatt tcgacaccac caaatgcata
      901 ggcatgattt aatttatcta ccgttccata acctacgata ttcgtataaa tatctcggtc
      961 caaactgatg atcgttgatt ttttttgttt cggattaact gttactacca tcatcgtatc
     1021 agaacgtcct tgttccgttc ttcctaatcc accggtatct aaacctaata gcaaaaccga
     1081 aaaaggttct ttgtcatcaa tgcttactct ctcagaacgt ttggaggata ttcgattaga
     1141 gtttttagaa attttgttta ctgtttggct cgcttctccc ataaatctga ttccgtatgc
     1201 tgataatccg gcaatcagca aaacaaatac ccctaacaag gttaatagaa ctttttgcca
     1261 tcgcttcata atacacctct gtttcacatt ttctaaaata ttgtctcata taactgttca
     1321 atcagcaaat tcacttttta atcataagaa aactttaaaa aacatttttg tgtaacgatt
     1381 actatttttt aaaatttctc aaaattctta actatcatac cataccgtga aaaagatcga
     1441 aagaccgaat acacgcagtt cgcaatcgtt ttatcttttt ttacgtattt tttccagaaa
     1501 aagcgaacaa caaggtattt tccaaataat cgtgcagtaa aatgatcgca catacctttc
     1561 ccatcttttt aacagtcttt gcctaccgtt cagattatca ttcgatttca cgaaaatttc
     1621 ttcctatgac tggtcctttt gttaaaccca tcttctattc gttttatttc cagctcactg
     1681 catggctctt aaaatgactc gaactagtga tttcattacc atcatctttt aatggataga
     1741 tatgctcctc ttcaaagtaa aacgattttc ttttaaagta atagaattga catggtaatg
     1801 agtatgtata ctagtggttt cacaaatggt cttttattca acatccaaaa actgaaattt
     1861 ctttaaaatc cattactaac gaagaaaatg ttccttgctt gataattagt taggacatgt
     1921 caagtgaaca aatgcattta cttagccaaa aaatctaaaa acttcataaa tagacaaaaa
     1981 gacacgactc tctaagaagt cgtgtcttta ttcaacaaat tattataatt tagtcacttc
     2041 agcagcttga ggtccgcgtg caccttcaac aatcgtgaat tctacttctt gaccttcttc
     2101 taggctcttg aagccgtctc cttgaattgc tgagaaatga acaaatacgt catctccgcc
     2161 gtctactgta ataaacccaa atcctttttc attgttgaac catttaaccg ttccgtgttc
     2221 cataaatgta tagcctccaa aaaagttctt gcaagtaacc ttgcaagaac attataccca
     2281 atttagaaaa cgctagcaag ctttcgctct tttattttcc aaattatttt taagactgat
     2341 atgacggagg aaatggttat tttgctgctt cgaaacgttt gtttacttca tcccaattaa
     2401 taacattcca gaatgctgca atataatctg gtcggacatt tttatatttc aaataataag
     2461 catgttccca aacgtccaaa cctagaattg gtgtctttcc ttccatcaat ggagaatctt
     2521 gatttgcagt agaggtaata gctaattttc cattttccat tacaagccaa gcccatccag
     2581 aaccaaatcg tccagcggct gcttttttga attcttcttt aaaagaagaa aaatcaccaa
     2641 aagcttcatt aatcgcttct tttatttctc ctgtaggttc gccaccagca tttggtgcca
     2701 tgatttccca gaaaaatgaa tggttagcat gtccgccacc attgttacgt acagctgtct
     2761 tgatatctgt tggaatagcg tccatatcag ataataattc ttctattgtt ttttcgccta
     2821 attctgggta tttctcaatt gctgcattta aattcgtcac ataagtattg tgatgcttat
     2881 catgatgcag atgcatcgtt tcttcgtcaa tataaggttc caatgcgtca tatgcataag
     2941 gtagatctgg taatgtgtaa gtcataaaaa aattcctcct caatgttttc tttataatgt
     3001 ctacattaca aagagtaaca aagagaagga aattcttcaa ttgttatgcc tagtcgtctt
     3061 tttcactttg acctacttct tcttcaaaaa cctcagtttc tttaaaaata aacaacttgc
     3121 tgaaaatata gttcgctagg ataacaataa tttgagtgat gattttcgcc acatactcat
     3181 tgctgttcat aacattgatc aacaatacca ttgcccccat gtccaatccg taagaaagga
     3241 tacggtagaa gaagaactta ccgcattcaa taagtgcatg tgtaaaattc ggtgattttg
     3301 aatggaatac ccaaagtttg ttcgttacaa aagcaaacaa tacagaacaa aaccacgaaa
     3361 tagaattgga taaggccata cttaaatcaa aatattggcg tgcgagagca aaactaacaa
     3421 aatttacaat cgtagccaaa ccaccaaaaa agagatagac tagtatctcc caatatcctt
     3481 ttgcttctaa atatcctttt aactgtcgat actgcttcat gagccaacct accttattaa
     3541 aaattcttat ttatcatact ctatatttca ataactttca tctttattta aaaaaattaa
     3601 ttttttttga tcctttgcct ttgtctctgc ttggatcgtc acatgtgtaa taccatattt
     3661 taattttaat aaagaaatta tacgaggata tatcttttca atctcgctta gtagctgatc
     3721 ttcaaaatca atatgtccgt cgaaaataat tatcttttcg ctatactgcc aaatatggac
     3781 atgatgaaca ttctttaccc catcgattgc ttcaatttct tgttccattt tttcatagtc
     3841 aatagcaggt gcactttgta tcaaaatagc ggcagctttt ttgatcacca ttatcgcttc
     3901 tatcaatata tatatcgaaa tgatcagtgt aataactgga tcgatccagt agatatcgaa
     3961 aaaacggatc aaaattgctc cgataatcac acttaccgaa gacagtgtat cacttaacaa
     4021 atgcaaataa ctagatttta tattcaaact ttcatctgct tcttgtcgta ataataatac
     4081 tgagaacaaa ttcgcgagca atccaataac tgctactgtt agcatcaaat gactatttat
     4141 cttttctggt gaagtgaacc gcttgaatgc ttcaacggtt aaaaacaagg ataatatgat
     4201 caatatgaat gagttgatga aagcactcaa tatttctaaa cgctggtagc catacgtacg
     4261 ttttttattt gcttcttttc gggccatttt ttgtgccccg taagaaaaaa taattgccaa
     4321 tgtatcacta aggttatgga aagcatctga aacaagagac aaactccctg acataatccc
     4381 tccaataaat tctgataaag tgatcaccgc attcaaaacc actactccaa atagtttttt
     4441 tgatgatctc tgtggctgtg taggcactat tccttcctcc attttattta tattggatag
     4501 tctttagaca agaataacat ttttttgata tttcaccaat cattcagatg tttccctaaa
     4561 gcacttgtat ttcacttgat acttcgctag aattgcaagt gatgacacta ctagatgagg
     4621 agtacttatg acaacaaaac aattaattat cagttccttt atacgattca aagacctaaa
     4681 ggagacgaga aatgtcccat tttggaaagc cattgtctat cttttagttt tgagtataat
     4741 tatggctttg ccaatctctt ttcaagtttt tcaagtctta aacaacattc aattggatgg
     4801 gcaaaagatt gctactcgca ttcctgattt tacaatccaa aacgggcagc ttcaaaccga
     4861 agaaaaagaa ggattcattt accaaacaaa ctccattatc ttcacatttg atccagaggg
     4921 aaaacgaaca gaacaggaca tctctactga tttgatgggg aattttgtta gtgtcgggat
     4981 gttaaaaaac aaacttatca tagctcttcc taatacaggc acgacttctg ctttgttgaa
     5041 caataaccaa ttagaacttc cctacaccaa tgaatcttta aaaaacttaa caggtaagga
     5101 gctccgttct tttttaagta aagcatctat accactttgg ataaaagtcc tgacatttat
     5161 attttctatt tatcctagtt ttctgaatct gctgatcact ttgctattta ccaacattgc
     5221 tgcttttctc tatgcacgct ttcgactaac aaaagctact tttcttgatt gtttaaaaac
     5281 attgatctat tcaatttcat tgcctactat aattgcaaca atcttgatgg tctttcttcc
     5341 ttcttttgac acaagcgcct tcattgcttt tgcaggaatc tttatttttg ctcaagctgt
     5401 caaaggatgg gcaaaaatat cgatcaatta agaaataaag acaactcgtt atttctactt
     5461 caatagaccc ttttagaggt tgtgacattt ttttgtcaca gccccttttt tatttgaaaa
     5521 atatttcttt ctcaaaaaac aagcaaggcg cttcaaagtt cttcttgtat gcgctacact
     5581 tagagtaaag aaaggcggga aattaactat gtcagaaaga atcatttatg cagatcgtcc
     5641 atttcgtgat gaatttatcg aagaaatcca tactatagcg cctggttata tctttaaaac
     5701 agaattacaa ccagaggatc tacaaaatgt agagatcagt ttgggctgga actcagacta
     5761 ccaagaaaaa ctattagcct cttctacctt gaaatgggtc caatctatct cagctggtgt
     5821 tgatcagcta ccccttgaga ctttttcaaa gaaacatatt ttactttcaa atggtagtgg
     5881 catccacagt gaatcgatta cggagcatgt cttaggaatc atccttgggt attctcgtgg
     5941 tctttttcaa gcacaaaaag cacagagtga catgaaatgg ttaggttcat cggtccatta
     6001 ccaatctatc caaggaaaaa aaatactaat cattggaact ggccatattg gaaaaatgat
     6061 tgcaaaaaaa tgcaaagcat tagacatgaa ctgctatgga atcaatacat ccggtcatcc
     6121 agcagaagga atggaaaaga ctttttcttt aaatcaacta aaggaagttc ttcctgaagt
     6181 agatttcgta gtcaatatct tacctctaac ggaggaaaca actgggctct ttgatcagca
     6241 tatgtttgaa cactttgatc caaaatctgt attcatcaat gttggccgtg gtgcttctgt
     6301 taagacacaa gatctggtgc aggctttaaa taaccagcaa ctttcctttg cagcattaga
     6361 cgtttttgag gaagagcctc ttccacaaga tcatcctctt tggaaaatgg acaatgtact
     6421 gattacttcc cacattgcag gtttgacacc tgattttcaa aagaaattaa tggctatttt
     6481 tttaaccaat ctgaaaagct actttgccac ccatgagctt caaaccaatc aagtaaaatt
     6541 gtctgcagga tattaatagc tgaaaacaat attttcaaaa gaacaaagag aaggtgacaa
     6601 a
//
LOCUS       DACEMS010000123         6452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEMS010000123 DACEMS010000000
VERSION     DACEMS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.128"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..550)
                     /locus_tag="IW735_002337"
     CDS             complement(95..550)
                     /locus_tag="IW735_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045306.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(704..1912)
                     /locus_tag="IW735_002338"
     CDS             complement(704..1912)
                     /locus_tag="IW735_002338"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1045307.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2024..2356)
                     /locus_tag="IW735_002339"
     CDS             complement(2024..2356)
                     /locus_tag="IW735_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1045308.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2504..3379
                     /locus_tag="IW735_002340"
     CDS             2504..3379
                     /locus_tag="IW735_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1045309.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3461..4042)
                     /locus_tag="IW735_002341"
     CDS             complement(3461..4042)
                     /locus_tag="IW735_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1045310.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4048..5307)
                     /locus_tag="IW735_002342"
     CDS             complement(4048..5307)
                     /locus_tag="IW735_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1045311.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5309..6355)
                     /locus_tag="IW735_002343"
     CDS             complement(5309..6355)
                     /locus_tag="IW735_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045312.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1798 a         1334 c         1100 g         2220 t
ORIGIN      
        1 tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat aagacacatc
       61 tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat
      121 cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt tcgctgattt
      181 ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca
      241 caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca
      301 atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg
      361 cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt
      421 cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat
      481 gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt
      541 ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag
      601 cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac
      661 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
      721 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      781 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      841 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      901 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      961 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
     1021 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
     1081 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
     1141 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
     1201 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
     1261 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
     1321 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
     1381 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
     1441 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
     1501 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
     1561 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
     1621 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1681 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1741 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1801 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1861 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1921 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1981 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     2041 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     2101 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     2161 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     2221 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     2281 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     2341 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     2401 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     2461 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     2521 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     2581 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     2641 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2701 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2761 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2821 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2881 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2941 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     3001 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     3061 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     3121 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     3181 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     3241 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     3301 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     3361 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     3421 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     3481 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     3541 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     3601 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3661 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3721 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3781 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3841 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3901 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3961 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
     4021 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     4081 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     4141 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     4201 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     4261 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     4321 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     4381 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     4441 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     4501 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     4561 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     4621 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4681 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4741 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4801 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4861 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4921 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4981 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     5041 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     5101 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     5161 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     5221 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     5281 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     5341 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     5401 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     5461 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     5521 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     5581 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     5641 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5701 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5761 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5821 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5881 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5941 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     6001 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     6061 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     6121 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     6181 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     6241 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     6301 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     6361 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     6421 aaagctcacc tcataacttg gaaataaata aa
//
LOCUS       DACEMS010000124         6304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEMS010000124 DACEMS010000000
VERSION     DACEMS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.129"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(365..1156)
                     /gene="msrA"
                     /locus_tag="IW735_002344"
     CDS             complement(365..1156)
                     /gene="msrA"
                     /locus_tag="IW735_002344"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1045313.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1347..1982)
                     /locus_tag="IW735_002345"
     CDS             complement(1347..1982)
                     /locus_tag="IW735_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1045314.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2248..3300)
                     /locus_tag="IW735_002346"
     CDS             complement(2248..3300)
                     /locus_tag="IW735_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1045315.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3313..4251)
                     /locus_tag="IW735_002347"
     CDS             complement(3313..4251)
                     /locus_tag="IW735_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1045316.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(4702..5352)
                     /locus_tag="IW735_002348"
     CDS             complement(4702..5352)
                     /locus_tag="IW735_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045317.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(5452..5961)
                     /locus_tag="IW735_002349"
     CDS             complement(5452..5961)
                     /locus_tag="IW735_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ1045318.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
BASE COUNT         1997 a         1310 c          925 g         2072 t
ORIGIN      
        1 tagttattgt ttgcgctttt atacggaatt tagttcatca ttaatgtgga attcgattag
       61 gtgtctatta attatcaaga tgattctcta caaaattcca tagaaaaatc aagattctac
      121 aattcgaatt ctctttcaca tggaaataaa ggctagtaaa aaaacaggag ttgtgacaaa
      181 agacttgtca tacctcctgt ttccttgcga cttgaagctg aatgctttgc gccacaacct
      241 tttcctcggt gttctcaaag caactcctcg aaataattca taactgtcca aaaatatgaa
      301 aagctatttt tgtcagttgc tcattatttt gtcggagctg aacgctttgc gccacaacct
      361 tcctctatct atgtcgctga aaaaataaat gaagctgatg cgaatgcttg atctttttat
      421 atcttttagg ttgcttctga taaaattgtt gatgatgatt ttcagctggc caaaaagtct
      481 ttgcttggcg aatctctgta acgataggac ggctaaatcg gttagattga atcagttgtt
      541 ctcttgattt ttcagccaat tgcctttgct tttcattaac aacaaataga attgaacgat
      601 attgtttccc ccgatcttga aattgaccaa aggcatctgt agggtctgaa atttgccaat
      661 aaatctccaa cagctcttca taggttattt gacgtgtatc aaatatgatc tcaacagctt
      721 caacatgccc agtatatcca ccactgactt gatcgtaatt tggttttttg gtctctccac
      781 ctgtataacc agataacact gagattacac ctggttttgt ttcaaaaggc tcaaccatac
      841 accagaaaca gccaccggca aaaatcgccc gttcttggta taagggtgaa gttatcttat
      901 gtttggcata atcataagcc tttgcactcg ctgactcccc tgtgattctt aaataaaaat
      961 ccatcgcatc tggtgtcaaa ttatctcgta gtgctagcgg acgcaagttg acctctagtt
     1021 tttctatcac ctgctttatc gacatttttg ctaggtcttc ttttgcctct tcaaattgtg
     1081 ttctttccca ttctcttgta tttcgattta aaataatatt ataaaggtct atttccatct
     1141 gctccacatc taacatgaag tcaccctctt tcaagttttc ttcttattca ttcaaaaaat
     1201 gactacctga aatttagcac aacgcataac aagttaagac taacttgctt ttcgctctcc
     1261 tcatctctat tcttagtcga acacatccac acgaatggtt acccatagag aaattctttt
     1321 acttaatcag cttcacactg ctttttctac tttcgtagtt gaaaaaaact taaacaaatc
     1381 atcggcaaat agttaaagaa aaaacttaag cgttgaaaga agcccaattg cttaaagaag
     1441 gcaaactgac taatcgacgg aatccgtgct aatccgtaaa gacctgcaat tagtaaacta
     1501 ataactgtta aaacaagata aaaatggcta agcgtacctt gaccactttg acgataaagc
     1561 agaacaagta acaatgggaa aagtagaaag cctgcatagc ctagcccaga acctatatta
     1621 tggatccaag tcgatagatt ccaagagctt tcattcgtat tgatactgaa taacccagta
     1681 aaaatacaat cacctatccc atataaacca actgttgcaa cagcaagtat tgcaagtgtt
     1741 tttgatgttc caataaatag gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca
     1801 gatacaactg accagaccaa gaaagcacgt ctgactggac tatctacgtc accaaacaca
     1861 ctaattacgg tagtcatttg attcagtttg ggataaaaaa ggccaaggat atacggtgtc
     1921 gataaatcac taatcacacc taataataaa aaataaaaac catatttttg caaaaaaatc
     1981 attttcttct cctacttttt agtgattatt cgatacatcc aacaatcaaa ttttttctca
     2041 ttttaacaca tatttttatt ctagttgcct tttgaacaat caataacata aaggtgagtt
     2101 tcttctacat tttaaaaaat cgaatggcac tgaagagttc agttgtcagc atcaatctcc
     2161 tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga catctcactt acgcatccat
     2221 tgattgaaaa tttcttagta aaatctacta gcctaaaaat ttgaagactt cttctcgatc
     2281 atacagttta aatactttct cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat
     2341 gactaaccaa caaagaacaa caagaaagcc atttagccct gcatctaaag aagccaacgg
     2401 accaacaagt ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag
     2461 agcgacagga atagctgtaa tcactgcact aactaaacaa ggaataagaa tcacaggttt
     2521 tctaaataga ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc
     2581 tgacttatta accttccaag agttcactac taaaacaaaa gtggttgcag ctacgcccat
     2641 cgcagccgca ccagcagaaa caccatctag ttggattgcc attccgattg ctactgttga
     2701 aataggagag ataatcaaaa aggcaaagga acaaccaatc aagatagaca ttaacagcgg
     2761 ttgtaaggta gtgaagttat taatcaatga tccaattgca gaagtaattg ctgttacaaa
     2821 agggaataaa taaaaaccaa gtaaacctgc acctacacct acaacaatcg gtgttaacac
     2881 aatcgacact gacccaaaac tatttccaat cagtaaaatc agcccgacag caatcgatgc
     2941 tgtcagcatc gtattaataa tatcgcctgt accagcacca acgtaaacac ccgtttcacc
     3001 caaatcaggt gtgaatttga ctacaccaga accaacaaaa gaagctccgg ctacaaccat
     3061 cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt
     3121 tgctagttga aaaataactg ctaactgtgc gatcgttacc gcaaagggat acgcactaaa
     3181 atattttaaa atggcaccta atactgcatt aggaatcaat ccaataatga ttcctgttgc
     3241 tgtaccagcg aggactttat tcagaaaaat tttcggtgat attttttgtg aagtttccat
     3301 tcaatctctc ctttatttta cgttcaaact atcttctttt gcatggacaa gctcagtcaa
     3361 gaaatcacaa taaggcgtcg caacaccata tttcttccct tttctagaaa ttgcgccatt
     3421 gatataatcg atttctgtta atcgatggtt cttaatcaag tcttgataca ttgaaggata
     3481 atgtaaccca attgtttccg ggtcaaaaca agattcacaa tgtgcaatga cttctgggac
     3541 atctagttca atcttctcta ctgctgctac tttggcaaac tcattgacaa tcgtcgccac
     3601 cattttatga gcagtcgatg tttttcctag ctcagccata ttaacgtcta agattgtgca
     3661 tagtccgttc attgttccgt tcacacatgc tttgcgataa atagaatagt gaatattgtc
     3721 agaaaaatga gcatttaatc ctgattcaga caatttgtct gctaattttt ttgcagctgc
     3781 ttcttttcca tcacctaaat tttgtaattc tactgaacca cttccaaata atttgacctg
     3841 accaggacct tcaagacctg ccgtccacat ggtatttcca atatagatat tttccattgg
     3901 tacaaatttc tcaataatat cttcatgccc gataccattt aatagacata agacctctgt
     3961 atcttttttg attaatgatt ggatatcctg cagcattttt tccagctgca tcgctttggt
     4021 aaatagaata atcagatcaa cttgatcttc tttttctact tcggattgaa gaacgattgg
     4081 tagttttgct tctacctctt taccattaaa atttgcttgt aatccatgct ctttgatttg
     4141 ttggacatgt tctgcccagc catcgattaa taatacttcg ttcccacttt ggtgaagcat
     4201 taacccaaag cgacttccca ttgcgccagc gcctgcaatt gctattttca ttgattcttc
     4261 ctcctttttc tttctattat attcatttga gaatcatttc acaaccttgt tttgttattc
     4321 ttctcttatt tatcatgttt atatccacca ttcaagttat tttatcagca aatcaataaa
     4381 aactggaata gaatcccaat ttcatcgtaa gagaaactag actagcaata agaaaataga
     4441 aacattcata gaaaaaacct cactcctctg attataaaag agggggcatt aacctcgtgt
     4501 atactaaaac cttccattca ttcgatggat gattttcttt ttgtgaaaaa atattcattg
     4561 tagtacgatg ttgcgcaaca cagacggatg gtattgctta ttatttatta aaatcgaaca
     4621 atacacttat ttaaaaactt ctttcccaga ttttcttcca tttttacagt tataactagt
     4681 attatcatga aaaatagcct tttattgtct atgggaataa taatttaaaa ttggtacttt
     4741 tttattaatt agttcatcgc ccaataatct ctccagtttc ttagcatctc ctgactcaac
     4801 cggaattatc caagcccagt ccaaataatt ttttaaaatc tcatcatatt ttgcaaatat
     4861 ttcattgcca tattccgtcc ttaaaattat attttcagtg ccagacaagt gatttgtatt
     4921 gagttgataa aaaagatctc catctaaaaa aatatcatta gaagaagatt tccccacagt
     4981 aatgaccatt ccatcagctc tatgtgttac atacaaataa ttgtatgacc tcatttcacc
     5041 taatgaatca attatttctt gccctttagc tctaatatat tgatctctat tacctatctc
     5101 actcttattt aacaaaagtt ttttcatgtc aaatttaatc ggctgtttta tttttgtaaa
     5161 ttcgcttcta ctactaatcc aatccgagat taatttttca acatgtccca gagtaaactc
     5221 tgatttattc gctaaatact ttttttgttg tattaaagac atctttcccc gaagctcaaa
     5281 attgagccta catcgattct ctatctcttc aaaaagcatc ttatcttcat cattcttata
     5341 ttcatcattc ataaaaccaa tccagctcct cgattaagat aatattttaa tctcatacaa
     5401 aaactagctc tcatcaaaac aaccattgtt ttgatttctc atgaaatttc tttatacttt
     5461 tctccgatta taaattccta accccttgag aaaattacgc atatatttat ctccgcaagg
     5521 cttaaaattt cgttggcctt cttttctcaa aaatgccccg atttcgcctt ttgatggata
     5581 gagttctgcc tcagcaaaaa cttctaaaat atcatcagtg gtcatagaca aggcgatttt
     5641 taattttttt agcaacacat tgttgatata tttgatttcc ttgctttttt gcggcgtgtc
     5701 tactatcggc ttatcttttt gcggaccacg cgccaagaga atcaggccat ttaggaatgc
     5761 gtcaaaaact tggttgttga tggttttttc atacacatta ttttctgcat tatctttgtt
     5821 cttatcctgt actttggtca gtaccgcctg agcttcttct ttagtcagtg gtacaccgcc
     5881 tagctcaaaa gctttaatca aatccgtatc tttcaaatca atcgcataac gcaatcgcaa
     5941 taacctatca ttgttattca tttgttcctc cgaagtattt agtctttact ttcttacgta
     6001 ctttttgtct tgctaagatt tttaggatca atgataaaaa ccgcatcact tatgatacgg
     6061 agctgggtat catgagtgaa tgatatttat aaatcactgg atattgaagt atattctgtt
     6121 ttctcaactt atttagtcag attttttaat gaagattttt tgagtaaaat ttctataatt
     6181 cctacttatt gaaaagtagt ttgtagtgct gcttctacct ctctcatctg ttctacaaat
     6241 tccaaataac ttgacgcagc ttgcggaatg aaaaaatgat atgtgaatat ttctcctcct
     6301 agcc
//
LOCUS       DACEMS010000125         6255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEMS010000125 DACEMS010000000
VERSION     DACEMS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.130"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..300
                     /locus_tag="IW735_002350"
     CDS             <1..300
                     /locus_tag="IW735_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1045319.1"
                     /translation="NEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYLPG
                     EDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(2235..2900)
                     /locus_tag="IW735_002351"
     CDS             complement(2235..2900)
                     /locus_tag="IW735_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1045320.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            3026..5062
                     /locus_tag="IW735_002352"
     CDS             3026..5062
                     /locus_tag="IW735_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1045321.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            5066..5704
                     /locus_tag="IW735_002353"
     CDS             5066..5704
                     /locus_tag="IW735_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1045322.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5732..6160
                     /locus_tag="IW735_002354"
     CDS             5732..6160
                     /locus_tag="IW735_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045323.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         2078 a          939 c         1144 g         2094 t
ORIGIN      
        1 aatgaagata tctttgtcat taaacgtcgc tattttacac aagcgagtga tgatttgatt
       61 catgtctttt ccaatgagtg gtttgtttct gagaaagaat tacatgcttc taatctgcaa
      121 tatctaccag gtgaagatcc aattcctaat atgaaagcaa ttatcaacag taaggattac
      181 gaaggatata aagccaaaca tccagaagcg aagccattta aatacccaca agaaatgaag
      241 cgagcatggc gtaaaatgtt agatgatgag ttgattccac ttgagaatga attgaggtaa
      301 acgagaagag ttaagaaaat tgtacgattc aaagatggta gtcaagtgga agtgtagaaa
      361 atagggatag atgtctttta tttgttcaca ttttataacg aagtatttaa gacagctatt
      421 gaataatgtt tcctccacct ttttcattca tttaccccac cccgttgtta tatgaccgtg
      481 ttttgagagg aaccaataaa aagcataggc taactagggg ttcctttttc ttttgttttt
      541 ttggaatccg cttaagtgaa atgtatctga aatgaaacgg agataaagtg attttttgct
      601 ataatgacaa ggttttagct gtttcagata ccatgtaacc caaaatagtt tattgagaaa
      661 ataggagagt atttgatact aactagtagg aaaaaatgag ataaaaatta aatggaatca
      721 tttcaggtaa tctcaaataa aaaaatatac agtcctgtgg cactttacct atgggactgt
      781 atatttttta ctctcagcgg gcttgttttg actccgtgtc tgactccgtc gtattgattt
      841 taaatgatat tcgatgacca tttaacaggt catttttgaa atttttgtct ctatatgact
      901 cagtttgata attattgata gatcaatttg ggtctatatc gaacaaggga ataatccaat
      961 aaaaaatgat ttagaatatc aaaataacaa aaaagaggac tacctgtatt cgtcctcttt
     1021 tttgaccaga ttttgaccag tctaatttac attctaatat attcatatga gctaattttt
     1081 tgattaaatg gtaagttttt ccttgtttgt tttagtttaa actagtgtgt aatttaaaaa
     1141 tctatatgga agggtctgtg gggtaatact taaataaagg ctaggaacct gataacatca
     1201 aggttcctag cctttttata ataaaatgaa gcgaaaataa tttcttttaa agagccgaga
     1261 ttatcagcta tataagagtg aatttgatgc aaaaaacaga gccgaaacag tcttggtgtt
     1321 cgtagctctg cttatatgtt tcgaatttaa gacaaagagt tggcaaaaga tgttgataag
     1381 gaagataaga taactgtgtt atataaacta ggtaactaac tgtaacaagc taattttaag
     1441 agattcttat tagaagacgt ttaggaatat aactaagaaa atgattatac aggtaattgt
     1501 catttcaata atgttttcta attttcgcgt ctttgtttta agaaaaaggc cagaatcagt
     1561 tggaaaaaag ttcgtacgat agtgaagagg ggcaaaattt cctactaata ttcccactaa
     1621 caagagataa aacggccagg ttggtgtgat tcctctataa aaaaatgccc atgctaccga
     1681 aatcccatag attaacaact gaacaatttt tacaatgaaa aaattgttct gccattactc
     1741 aaagtctctt cctccttgta tcgtttattt gtttcatttt atcattatga taggcgcttg
     1801 aacaatccat tttgtatttt attgagtact cttggataaa ctaccagcga ttatcatatt
     1861 tatttatgtt actgtaagcc atcatataaa agtaaatgag cagatcatag aaatataaaa
     1921 aagaactaaa attgatctaa attatgtgca gcttgactac gtaccaatct atattactta
     1981 ctagattggt cgttaatcta ttcggatcga atgagcaaaa tatttttgtg aatgtccatc
     2041 cttgattatg attccaaaaa gaatcattgg gaggctgtgg cgtaaatatt atactataag
     2101 ccagaaatac ctatcattct gaactttttt atttaggaac tttcatctca aggctttttc
     2161 aattaatttg tacccctgtt ttttggataa gatcatgtgc attgattttt tctatctcta
     2221 cgttcatctc ctacttaatg cctatctggc tcctgagggc ttctttttaa ggcgtcgtca
     2281 tagtcttggc tgaggatttc ttggtaatct gagggaatgg cttcccaagt gagcaggcga
     2341 acgttcatga cgtgttttcc tttgtgtttg attacagcat ggtttttctc tttttctacc
     2401 tctccagcgt gaaaagacag ccatgtacca tggccaaaga tatcattaca tacccaacgt
     2461 tcgatggaat tgtccacgta tcctacaggg agtgtggtat agccgatttg tggtttcaaa
     2521 aaaggattga atcgatcgat cgtttcattt ttgtattgat aattgataag aaaaagatag
     2581 accaaacaaa agacgagcag aaagaaagat agtgtctttt tgagtgagaa gggttgtttt
     2641 gatgaataag atttcttgtt aacaagaggt tcatctggca atgttggttc tattgattcg
     2701 gtagctgatt caatagaagg aaaatcaatc ggcagaccta aagtatgatg taaaagtgac
     2761 agcatttcaa agtctggcat tgtacgagaa ttttcccaac tagaaatcgt tttatcggaa
     2821 acaccaattt tttcgcctaa ttctttttgc gtaagcttac aatttctgcg gtaagttcgt
     2881 attttttctc cggtgttcat cttcttctcc tttcgattct acagtatata gatttattct
     2941 acatgaccta tacacttgat agatacgttt ttttgatgaa aaacggtatg attttatcaa
     3001 agaatcattt aagtaaggga gagtggtgat aatcaaaaaa aagttcacag ttttactcac
     3061 aatcatagtg accggtatca tgagtggttt tgccttggtt agtataaata aacaggtact
     3121 ctatcggaca aatgaatttt tccatcaaac agatacttat actccgattc gattgccaca
     3181 acattttatt gggcaagaag aatatgagca gctaatcatg gtatttgaaa atgtctcatc
     3241 agaaactgag ctttcttata tgaaaaaaac agtaaatgaa tccaatgaag tagatacatc
     3301 ttggaacttc aagatacagc caaagcttga aatgacattg tattatcaag gggatgtcaa
     3361 atcttctcac gagaaggtac cctattctaa tacctccttt cgcgtaacgt ccattgaaga
     3421 atcgatcaaa ataaaagatt ttgaaggtga atttttcttg atgacgagag aaaaagaagc
     3481 ctatgaacgg gtcatttcac tattcactga tcgattcaat caagcatttc acactgatta
     3541 tgccactaat atttttgaag actttgaaaa tagctattcg tattcggtta tttcaaatac
     3601 agacgtatat aattatcgag tgtatttaac tattggattg attcttttat tccttttgtt
     3661 atcatccttt ttcttattga tgaataaaga aatcagttta ttgttgaata atgacttttc
     3721 attacatcaa gtattgtggt gtttattggg gaaaaagaaa atcattatta cattcataat
     3781 tgcaggaatc attggtggtt ttatttttat gtattataag gagcaatttc tttatttgtt
     3841 ccagatgtac gttttcttgt tcagcggaat atatggtatc tctttgattt ctcttttatt
     3901 agttgaaaaa atcgcttcgt taagagaagc gttcaactat atccaataca ttctttttac
     3961 atttggattg ttgttttctc ttcttttgac cgttgccaat gtcgaattag caactatcgt
     4021 tttttcttct tcacagttag ctgtttccca tgaaccacca aaagaaatcg ctggtgagga
     4081 ctatcatgta ttttatccag taaccatcgg gaaaaaccac gtggatttta tttattccaa
     4141 tcgttttgca tcagcagcag atcaggaatt atatgaaact ttgaataaaa acggaagtat
     4201 attagtcaat gtatcagatt acttgaatga agaaaatgaa cagtttggca gagggataaa
     4261 aataaatctg aattatctta agaaatttcc tttgattgat gtctctggaa ggttgatcca
     4321 aataaccgaa aaagaaacgc accctgttat attgatccct gaacgatata gacatacaga
     4381 tttaaaaaat gacctggcgc aaataacaga atattatcaa gaaatcagtg aaaaagaacc
     4441 gaaatttcta tttataaaaa acaatcaacc catttataca tttattcctt ctatcccctg
     4501 gatcgaacat tatccggttg ttgaaatact gacattgaaa aacagtatgt atttggaaag
     4561 aaatattctc agcggagaaa tctatccacc gttaaagatc aagataggct ctttgtcgaa
     4621 agaaaggtta tttgagctga ttgaagaaag tcagttacgt gataacttgc agttatcatt
     4681 tccgtatact cagactgaag agatagctgt caaagtgtta agcagatcat ttcattactt
     4741 gatacagata tttttactca ctttcttttc tttcttcttg ttgagctatg tcatgttatg
     4801 catatatttt gtctacaatt gtcggaaaat cgctatcttt cgttcaagtg gttattcgct
     4861 ctttgaaacg tataaagatt tcttcatgat gaacctcatc aaatggggaa cgacaagtgt
     4921 aatcttcctt tttcttatag aaagagagcc taaatatctt tttaatattt ttttctttag
     4981 cttcattgat tttattcttt cagttgtttt tattttgtct acagaaaaga aaagtcagtt
     5041 attgctgatg aatggaggtt aacaattgga gccgttgata gtgataaaag gtgcgaaaaa
     5101 aatcattcga aacaaaacga ttcttcattt ggatcgtttc actgcggatg ctggggatct
     5161 tgtgactgtt gttggagaaa gtggagcagg taaaagtacg ttattgaata tattaggatt
     5221 gaatgatcag ttcagttcag gacaattcta tttgttcgat caagagacga aacaattaac
     5281 tgggagagag aagcgcttga taaaaagaaa taccatatct tttctttttc aagattttgg
     5341 gttgattgaa tatgaaacaa tcgattttaa tttagatatt ggattgaagt tttcgaattt
     5401 aaatgtttca gataagcgaa cagcaaagta tgaggcattg aaaaaagtaa atataaacaa
     5461 gaagctaagt acgcctatct acacattatc tggtggtgag aagcaaagaa tcgcctgtgc
     5521 gagagtctta ttgaagaata gcaaaatcat tttagccgat gaaccgactg ggtcattgga
     5581 taaaagaaat aaggataaag tagttgaaat cttatcgcaa caagcttccc aaggtacgtt
     5641 agttatcgta gtcacgcatg atccatatct gaccaaaata ggaaacaaga agatcatgct
     5701 ttaaaaatca aaaaggaaag gatgtcgttt tatgaaaaaa gcagttatta tttgtgcact
     5761 agggataggt tttgttggag gaaacgctat gtcatctgaa gtggaagcaa attcagttca
     5821 cggtgaaatc tattacaaat ctttaaagcc aggagagatt cattttgatg ctaaaggtaa
     5881 tattattaag tcaaatttac cagtagaccg ggaaatttct agaacagcaa ttaatcatag
     5941 ctctggaaga tgggtttatt atagtgatac aacttgtacg agaaaaacag gtcattctaa
     6001 ccactatagt cgtgtctata atcgtcacgg tgcaaaagcg aaagttggaa actctattcg
     6061 atcagcaaat gcaacaaaag gagtatgggc ctatgcaagt gcgacaggaa atagaactga
     6121 cacattctca tgttggtaca atccaaccgg ttggtattaa gtatccaaaa gtttatataa
     6181 aatagaaaaa acatataatg aaacagcgtt aaaagttatt tttggtagtt ttaatatatt
     6241 catttttagt tgtat
//
LOCUS       DACEMS010000126         6220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEMS010000126 DACEMS010000000
VERSION     DACEMS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.131"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            54..506
                     /locus_tag="IW735_002355"
     CDS             54..506
                     /locus_tag="IW735_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045324.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="IW735_002356"
     CDS             520..1209
                     /locus_tag="IW735_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045325.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="IW735_002357"
     CDS             1334..3685
                     /locus_tag="IW735_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1045326.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="IW735_002358"
     CDS             3765..4160
                     /locus_tag="IW735_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045327.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4485..5102
                     /locus_tag="IW735_002359"
     CDS             4485..5102
                     /locus_tag="IW735_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1045328.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5143..5442
                     /locus_tag="IW735_002360"
     CDS             5143..5442
                     /locus_tag="IW735_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045329.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5476..5643
                     /locus_tag="IW735_002361"
     CDS             5476..5643
                     /locus_tag="IW735_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1045330.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5688..6212)
                     /locus_tag="IW735_002362"
     CDS             complement(5688..6212)
                     /locus_tag="IW735_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1045331.1"
                     /translation="MDKLFQEKASGKDTERPEFQKLLNYIREGDCVIVTSLDRLGRDY
                     EDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIR
                     ERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGI
                     SRPTIYKIKKANDL"
BASE COUNT         2141 a          857 c         1244 g         1978 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     4321 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     4381 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     4441 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     4501 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     4561 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     4621 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     4681 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     4741 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     4801 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     4861 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     4921 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     4981 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     5041 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     5101 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     5161 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     5221 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     5281 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     5341 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     5401 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     5461 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     5521 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     5581 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     5641 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     5701 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     5761 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     5821 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     5881 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     5941 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     6001 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     6061 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg
     6121 caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta
     6181 ccactggctt tttcttggaa taatttatca atgttaagtc
//
LOCUS       DACEMS010000127         6077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEMS010000127 DACEMS010000000
VERSION     DACEMS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.132"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IW735_002363"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IW735_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1045332.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IW735_002364"
     CDS             complement(2203..4389)
                     /locus_tag="IW735_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1045333.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IW735_002365"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IW735_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1045334.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IW735_002366"
     CDS             complement(4611..5051)
                     /locus_tag="IW735_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1045335.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IW735_002367"
     CDS             complement(5125..5664)
                     /locus_tag="IW735_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ1045336.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1577 a         1368 c         1068 g         2064 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccaacccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgagg
//
LOCUS       DACEMS010000128         6012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEMS010000128 DACEMS010000000
VERSION     DACEMS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.133"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(367..729)
                     /locus_tag="IW735_002368"
     CDS             complement(367..729)
                     /locus_tag="IW735_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045337.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(803..1183)
                     /locus_tag="IW735_002369"
     CDS             complement(803..1183)
                     /locus_tag="IW735_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1045338.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1265..2440
                     /locus_tag="IW735_002370"
     CDS             1265..2440
                     /locus_tag="IW735_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1045339.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2517..3101)
                     /locus_tag="IW735_002371"
     CDS             complement(2517..3101)
                     /locus_tag="IW735_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1045340.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3302..4300
                     /locus_tag="IW735_002372"
     CDS             3302..4300
                     /locus_tag="IW735_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1045341.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4412..4921
                     /locus_tag="IW735_002373"
     CDS             4412..4921
                     /locus_tag="IW735_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1045342.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4951..5610)
                     /locus_tag="IW735_002374"
     CDS             complement(4951..5610)
                     /locus_tag="IW735_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1045343.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5612..>6012)
                     /locus_tag="IW735_002375"
     CDS             complement(5612..>6012)
                     /locus_tag="IW735_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HAQ1045344.1"
                     /translation="YLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPGAGSVIS
                     FVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRNNIDTLL
                     VLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
BASE COUNT         1927 a         1123 c         1055 g         1907 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
       61 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      121 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      181 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      241 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      301 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      361 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      421 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      481 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      541 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      601 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      661 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      721 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      781 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
      841 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
      901 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
      961 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1021 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1081 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1141 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1201 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1261 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1321 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1381 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1441 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1501 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1561 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1621 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1681 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1741 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1801 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     1861 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     1921 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     1981 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2041 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2101 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2161 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2221 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2281 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2341 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2401 acttaatcga atcaagtatg ctttagagac aatcgattaa atcatttatt caaaataaaa
     2461 ggacgagacc ttactaggtt tcgtcctttt tcataattac tccgtaccat accaaactat
     2521 tctgaaattt caattgtttc aatcactaca tcatgtacag gtcggtcttg tggaccacgt
     2581 tgtacatttg cgatttcatc tactgtgtcc atcccttcta gtacatgtcc aaagactgta
     2641 tgtcggaaat ctagccaagg agttccgcca cctttatacg cttcaatgat ttctgatggg
     2701 aatccagcac cttctagttg tccaagcata ttagctggca catttgtgtt atttacaata
     2761 aagaattggc ttccgtttgt gtttggacct gaatttgcca tagataaagc tccgcgaagg
     2821 ttgaacagtt cacgactgaa ttcatcttca aaactttcgc cataaatact ttcgccaccc
     2881 attcctgttc cagttggatc gccgccttgg atcatgaaat caggaataac gcggtgaaaa
     2941 atcacgccat cataataacc ttttttggaa agttcaatga aattttgaac cgtcttcggt
     3001 gctagttcag ggaataattg aacagtaatg tcaccacggt ttgttttgat aacagctttt
     3061 ggtccttttt cactttttaa atctagttgt gggaatgcca tattgtttcc tcctaatatt
     3121 tgcatacttc ataataataa taacataata cctaacctct cgtaaaaata ctagatttgt
     3181 tttcttttat ttttcatcac tagatacatt ctataactta ccgtttcgat ttcgaattca
     3241 aacatttttg agaagcattc tcaattaaat tctgctatag tgtgaaaagg gaggaatttt
     3301 tatggaaatc tatgacatta caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt
     3361 ttacgcagga atgcgccaag ctaaaacaaa aatcattgac agtctacctc aacttggtgg
     3421 tcaattagca actttgtatc ccgaaaaata tatttacgat attcctgggt atccagcaat
     3481 aaaagcaagt gaattaatcg atcaattaga aaaacaatta actactttta accatacatt
     3541 ccatttaaaa gaagaagttc tctcgcttac tcgtgaagac gaagttatcg agattactac
     3601 aaataaaggc atccattatt ccaaagcagt cattttagct ttaggaaatg gttcattcca
     3661 gccaagaaaa ttgaatttag ataatgctga atcatttgaa aaccacggat tggattattt
     3721 cgtgaatgat ttgatgaagt atgctggaaa aaaagtcgct attgctggag gtggtgattc
     3781 tgctatcgat tggacgttga tgttagagcc tattgcaagc gaagtttact tgattcatcg
     3841 tcgcccggaa tttcgtgcgc acgaacatag tgtatcacgc ttgaaatctt catctgtcaa
     3901 cttgcttact ccctatctca tcgatggatt atcaggcaat aatggggaac tgacagatat
     3961 ccggttaaaa aaagtgaaaa gtgacgagac gattgatttg atgattgatt ctctgatcgt
     4021 caattatgga ttctcttcta atttagaaca tctttcttct tggggacttg atagtaccag
     4081 aaatgccatt actgtaaaat cagatatgtc tacttctatt ccaggcgttt atgcagctgg
     4141 tgacatctgt acctatgaag gaaaagtgaa actgattgct actgggctcg gtgaagcacc
     4201 tactgcagtg aacaacgctt tacactatat taatccaaag gaacgaactc agcctggtca
     4261 cagcacaagc ttatacgata aaaatcttcg tcctagttga ttttttatat ctctttgcat
     4321 ttcaagttaa acccgaaagc agtttcttgc atttttttca tgttccgtta acatgaaata
     4381 gagaaagaaa aaataaaggg ggagcaataa tatggtcgtc aaaactgaga ctttagaaga
     4441 aaaggcaaga gaactattga aggaacgtgg cgtaactgta gaagatatcg cacaacttgt
     4501 catgttttta caaaaagact atatttctga tttgactttg gcggattgta ctgaaagtgt
     4561 caacagcgtg ctaacaaagc gtgaagtaca taacgccatt atcactggaa tccaattaga
     4621 tatattagca gaaaagaaaa aattgatgag tccgttgcaa catattatcg aagatgacga
     4681 aggcctttac ggtatcgatg agatcctagc cttgtcaatc gtgaatgttt acggtacgat
     4741 cggctttacg aattatggtt atatcgacaa ggtgaaacct ggtattttaa aagaactaaa
     4801 cagccacgat ggcgttcatg tccatacctt cttagatgat atcgtcggag cgatcgccgc
     4861 tgctgctgcc agtcgattag cacacaaaga accagaaaaa agaagtcgcc ttactcaata
     4921 attcaacaaa aaaagaaaga aaacgtctat ttagacgagt tctttctttt ttgtttgttt
     4981 caagctattt tttcctaaga aaagaaaaac agcaaaaaat aattctaata atacaaaaaa
     5041 taagataaag ctatgcatga aaaattcttc tggaagtaca ttgatgattg gatcagtaac
     5101 gggatcgaat aaccagtcat cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg
     5161 atcaaatcct atcatcatga aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa
     5221 cggacgaatc aaccgccaaa gccgatcatt cttttgcaaa tatttgataa acaaaatact
     5281 cgggatcaaa gtgatgaaaa acacagcata atccactaaa aacaaaattt tcacttcata
     5341 aaagtgatgg gctccgctag cagaaactgg gaaatctggc aagctgagga tggttttgaa
     5401 gggattattg agataattca ttaggtgatc aaaatttttc agtaacgtct cttgatctaa
     5461 agaagtataa tccaaaattt gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt
     5521 gatcgtcaca ccaactgcca gactgatcag tgtcagaaat aggctgaaca atccagcata
     5581 ttccatccac tgccatctcg aatttttcat attagaagct ccattcatcc agtgattgtt
     5641 tcacataggt agctgggata ggcaacccag gtacatcttc ttcttttgta aaacctgata
     5701 aaacgagcaa agtatctata ttattgcgta tcccagcttg gatatctgtt tcatagttat
     5761 ctccaaccat gattacttct tctttttgca gccctaatac ttcaacggct ttatccataa
     5821 tgatggcttc tggttttccg atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag
     5881 agatgactga ccctgctcca ggaagaaggc cgcgttcagt aggtatattc ttatctgggt
     5941 tcgttccaat gaaagtagca cctttttgaa tagcaagtgt tgctttaact actttttcat
     6001 aggtcagata at
//
LOCUS       DACEMS010000129         5925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEMS010000129 DACEMS010000000
VERSION     DACEMS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.134"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(76..1170)
                     /locus_tag="IW735_002376"
     CDS             complement(76..1170)
                     /locus_tag="IW735_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1045345.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IW735_002377"
     CDS             1359..2120
                     /locus_tag="IW735_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1045346.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IW735_002378"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IW735_002378"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1045347.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IW735_002379"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IW735_002379"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1045348.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IW735_002380"
     CDS             complement(3760..4773)
                     /locus_tag="IW735_002380"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1045349.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IW735_002381"
     CDS             4938..5858
                     /locus_tag="IW735_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1045350.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DACEMS010000130         5905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEMS010000130 DACEMS010000000
VERSION     DACEMS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.135"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..665)
                     /locus_tag="IW735_002382"
     CDS             complement(93..665)
                     /locus_tag="IW735_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1045351.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(672..1334)
                     /gene="rpe"
                     /locus_tag="IW735_002383"
     CDS             complement(672..1334)
                     /gene="rpe"
                     /locus_tag="IW735_002383"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1045352.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1350..1865)
                     /locus_tag="IW735_002384"
     CDS             complement(1350..1865)
                     /locus_tag="IW735_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1045353.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1867..2151)
                     /locus_tag="IW735_002385"
     CDS             complement(1867..2151)
                     /locus_tag="IW735_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1045354.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2185..3513)
                     /locus_tag="IW735_002386"
     CDS             complement(2185..3513)
                     /locus_tag="IW735_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1045355.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3527..3982)
                     /locus_tag="IW735_002387"
     CDS             complement(3527..3982)
                     /locus_tag="IW735_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1045356.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3985..>5905)
                     /locus_tag="IW735_002388"
     CDS             complement(3985..>5905)
                     /locus_tag="IW735_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1045357.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1917 a         1147 c          836 g         2005 t
ORIGIN      
        1 ttattaagtt atgtctaatc aaataaaaaa accattctca tatttgctga ataattagca
       61 tttagagaat ggttttttat ctttaaaatc tactactcaa aatctgcatg tcgagcaaac
      121 atcgtttctg agctttcacc tgttttatcc attaaagata acactaaact atcaaaggtg
      181 agaaatgcta attgttcaaa acttgaaccc ataggttgtg aaaaagtatc ttcttctcgt
      241 tgattttcat ctttggtaat acctggtaat acaagaacag aatctgctaa ttgaccaatc
      301 gacgagtctt ttttcatggt aactaaagca atgcttacac cactttgatt tgctttggcc
      361 gctaaatttt ttaaactacc tgtttcacct gagccagaac agataattaa taaatctcca
      421 ggttgcgaat gtggtgaact aatttcaccg acaaggctga ctgaaaaacc taaatgcatt
      481 aagcgattcg taaatgcttg catggcaatt ccagagcgtc ctgcaccagc tagaaagatg
      541 tgtttagctt gaacaatttt ctcactaaat tgttctaact ctacagaatt aatacgtttt
      601 gagttatttt gtaattctaa gataatcgct tctaaatagt ttttattttg gtctttattg
      661 gtcatgatct tttacttcac ttcctctaat gattgattta gtttatcaat ttgttcttgc
      721 ggggtatttt tcccattgaa gatgaaaccg cctgatacaa aaatatccgc ccctgcttta
      781 gaacaaactt taatcgtttg attatcaatc ttgccatcta cttcaatatc atacgtataa
      841 tcattttgtt tgcgtattac atctaactgt tcgattttaa ctactgtctc ttgaataaaa
      901 tgcttattct ctgttcctgg attgattgtc attactaata cttgatcgac taaagataat
      961 actggttcaa taaaactgac tggtgttgca ggattaaaag taatccctac ttttgctcct
     1021 gcttccttga ctaactgaat gaccttcatt gcgtgtttgg tagcttcaat gtggaaggta
     1081 acaatatctg ctcctgcttc tacaaatctt gtgacaattt tttctggtgt atcaatcatc
     1141 aaatgaacat caaacacaac gtctgttagt ggacgaatat ctttaataac acttggtcca
     1201 aaagccatat ctggtacaaa acagccatcc attacatcaa tatgcaaata atcaacattc
     1261 ttttctttta agatttcaaa taactcttta acttctgcga taggtgcatt taacaatgat
     1321 ggagcaattg tcattctcta ttcccccgtt tattctccca taatcatgag attgattgtt
     1381 ctagttcgct ctcttagttg atcccaatcg acaaaataga cacggcccat ttctcctaat
     1441 aaaagtttac cttcttgaac aattaaagtc attgagttgg ttccataaat agatgaacgt
     1501 aaatgtgcat cggtatttag catgacccat ttttctgcag ggtaattggg atcagataat
     1561 cttgttccaa aatcaatgtg ttttggcccc ggactgttgt attgattttc actcgtcatt
     1621 tttggaataa tcgtttccat tacattgttg atgtctacat gtaaataatc atcaccaaaa
     1681 aagtttgtgt catgcatcgt ttcttcaaaa aagagtgagc aagttgtatg cgggctcgaa
     1741 attactaaca ttccattctc aatacctgct gtatcaactg tatctaaaag ttcttttcga
     1801 atatcatgat acgaaggacg ccctgaaact gtttcaatag tcaaagtgtc atgatgaaaa
     1861 cgcatattaa acacctaatc tttcttttac atctgcataa atggcatctg taccaatacc
     1921 tgttaataag cccaagccat tcgttacttt agctttaatt acatcaggga caattgttgt
     1981 acttactaca gcatcatatt catcaatttt atttagctca tcagcaattt tagattgata
     2041 aattttcact tgatctgtta atccttcttc ttccaaccat tttgataatt ttgctactgc
     2101 aattgtagaa gtagcatgac ctgctccaca aataactaat aattttttca tattgatctc
     2161 ttctttctta ttttaatttt atgattaagc tttcttcact ttgttcatgt agatagaagc
     2221 acctactaaa acaactccta atccacctat tccaattaca ccaaagtaag atgctaataa
     2281 aacaaacaac cctactggcc ataatccaca taacaacata gttactaatc ctgctgtccc
     2341 cacatcgaaa cttgctaatg taaacacatc agtgataatt ggtgccaacc atgttgccat
     2401 atataatgtt gtaacaacat acaacgaaca tcctactaat gatctcacaa tatttccttt
     2461 aaaataagga atcattaaac agatggcgaa agcaaagaat gctaaatctc caaaaggtaa
     2521 tactttattt cctggaagaa ttagtgctag caataatgag attggtacaa gcaatagaga
     2581 agttgacata atagttggat gacccacagt taacgctgca tccattccaa tgtttacttg
     2641 gcgaccattt aactttttat tcgtaaattc tttcgccgct tctgcaattg gcattaaacc
     2701 ttccataaac attgctacca ttttaggcat aatcttcatc actgccgcag tcataattcc
     2761 taagtttcca attcctgcta cgtcatagcc tgccataata cccactacaa taccgataat
     2821 taggccaata acaactgtgt cacctaaaac accgaaacgt tttgttaatg tttctgcatc
     2881 tgcatctatt ttattaaatc caggaatacg atcaaaaacc caatttactg gtttggcaaa
     2941 tagtactcct gataatccca tcatatgtgt gatagagatt cctggatagc cgaaaaattc
     3001 gctaacagtt ttttgtgtac gatctgctaa gaaccattgt aaaattactg cgacaatcgc
     3061 gccaattaag ccaaaccaaa agtttcccgt aacagattga gtaatcatac ctaggaaaat
     3121 tgcgaaccaa aagttccaaa tatcaacatt caatgttttt gtccaaccca aaaccacaaa
     3181 tatgaagtta agaattaatg aaattggaat cattaaaatt cccaatgatg tagagtaagc
     3241 caaaggtcca ccaacaccta cgccaatatc taggatattc aggccaccac cgtattgttc
     3301 ccccatttgt tgaattgctg ttcctaaacc atctgataat aaacttaaaa ctaatcctaa
     3361 accaatagaa ccgataccaa cagtaacgcc ggaaacaaat gctttaccag gttttaatcc
     3421 aaataatatc ccgattacaa aaataatcag tggtaaaaag acgtttgacc ctaatccaat
     3481 aaaccattca aaggcattta atacaatatc catctctcta ttcctcctaa taaactcctg
     3541 cagaatgtac taactcaata aatgcttctt tcgtggtaca tgcaagtaat tcatttactt
     3601 tttcttcatt ttgaaatagg ctaactaagt tttgtagtgt atcaatttgt tcatgaggtt
     3661 gtgacattgc caacataaag actaaacgta caggtatttc atgagattta tctgtcatat
     3721 ctgaaaatac cagtggttcc tttaatgagg caaaacagat ttgagagcta tttacatgtg
     3781 cggaatctgt atgtgggata gcaaacccaa tttgatttac ttctaatcca gttggaaatt
     3841 cttgttcacg gttggtgata ccttctaaga aactatcttt cacgcaacca gcgtttttta
     3901 attgttcaga cattacttga aacaattctt tttgatcttt cacttctaat tgaaataaac
     3961 ataattcttt atctagatac atttttattc tcctttttgt tgaaaaattc ttagtaattt
     4021 ttcgctatca tgaacttgta acaagcgatc gaccatttct ttttgcccaa taaattggta
     4081 aagttcttca tatactcctc taatctcaga tacatcttct tcagctaaac taaccataat
     4141 gactaatttg acttttcgat cgccccaatt aatcgcattt tttaatgcta caataaaaat
     4201 gttggattca atgaccattg atggattcgc atgcggtaaa gcaacaccac tttctaaatt
     4261 agtattcccc attttctctc tagagaagat agaagcacga aactcttcag taactaatga
     4321 attggcttct agttcggaaa tcatcaaatc caaacattct tctttgcttt ccacctgtac
     4381 tcctgttttt actaaagatg attgaacaaa tttagatatg gtgggaactt taaccgcttt
     4441 aggaatcagt tcattttgaa taaatcgcga aggttcttct attaaatatt tagtataagc
     4501 ttccataata ttcatataat ctgttttact taccaatgga gataccctta ctaagggtat
     4561 accacctgca tcaaaggaaa gagaggaaat aatcaaatca acttccccaa tttcagctaa
     4621 atgaaactta tctaaagttg atatttctac attatctttt gctggtaaaa accgtcgaac
     4681 tttattgtaa attaattgcg cagaagaaat accgtattgg caaataatga tgatattatt
     4741 tgctttggac tgatcttcaa tagcaatctg aaaatgaatc aaaattaagg aaacttcatc
     4801 gtccgttaaa gtcacatcaa attcttcttc aaggatggac atggcgtacc atactatgct
     4861 aaagagttct gaatattgag atttgatatt ttctaataac ggattttgaa ttttaatacc
     4921 tttttttaaa cgaaggacca ttgctggcac atgatacaat aaggattgat ataatcgatc
     4981 attttgagtc aaatcaattt tttcaatctc actcattctt tcaatcaatt tatgaacgct
     5041 catagcgtac tcttcattgc tggtttttaa attatttgtc acacgatgtg caaatagttg
     5101 acgacataga tattctttat ctgcgtcata aaatggaaga ttcaattttg ccttcaattc
     5161 ttcaattaaa ccatttgcca caatgtacgt ttccatatag cgaatgctat tgaagaggaa
     5221 ttcttcttca acagttaaat gatgaccttt cagtaaacgg ttacattgga taactaaagt
     5281 agaaatcaat gaccgaacat agtaatcaga aaccttttca gtcagttcac tgtaatcatt
     5341 gaatagcaaa tcatgaatga cactaataac ttttgaatca aacaacatat ttaaaacttg
     5401 aaaaaatgtt tttgattcca tgtcattcgc ataatagaat acaagttgct tgattgctcg
     5461 ttgaattgct tgttcattac cagtaaaact aattccttct gcagatgact gcaacttaac
     5521 ttctccactg ccaataattt tattgataat tctcaggtca ttgtataacg atgttttact
     5581 aaccataaaa ctttgagaca aatgagttaa agaaacaggc ttatcaccaa acaaaacttc
     5641 tttaatgatc tcgacacgtc ttttttctgg agaataaata tctttttcaa gataattttc
     5701 taccgatgaa atcaaatgtt caacgttttt cctatcacct atgagtttga tacccaaaga
     5761 aggtaatcgc tcaattgtta cttggtaatt aactacataa tcttccaaca attcaatatc
     5821 ttgataaatg gtcttggtcg acactgccaa tttatcacta aaaaaagaag cgggtttaaa
     5881 gtcttcttgt cgacttaata atttt
//
LOCUS       DACEMS010000131         5873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEMS010000131 DACEMS010000000
VERSION     DACEMS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.136"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            640..1374
                     /locus_tag="IW735_002389"
     CDS             640..1374
                     /locus_tag="IW735_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1045358.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1390..2559
                     /locus_tag="IW735_002390"
     CDS             1390..2559
                     /locus_tag="IW735_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1045359.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2575..3570
                     /gene="lacD"
                     /locus_tag="IW735_002391"
     CDS             2575..3570
                     /gene="lacD"
                     /locus_tag="IW735_002391"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1045360.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3649..4575
                     /locus_tag="IW735_002392"
     CDS             3649..4575
                     /locus_tag="IW735_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1045361.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4594..5844
                     /locus_tag="IW735_002393"
     CDS             4594..5844
                     /locus_tag="IW735_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1045362.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1966 a          955 c         1202 g         1750 t
ORIGIN      
        1 tttgaaaaat gctatttcga ggtagaagga tattatataa gcacggttga ctaaacgaag
       61 tgataataaa aaaagttatt caagaataag aaaaattatg atacgaattc agataaactg
      121 gagaatacag agacctttaa aagggataag aaacaacata aattcctctt aagagagaac
      181 gaaaggacaa agccagcaac ttgatacacg agctagtatt agaggaattt attctcatag
      241 actccctttt gaatacgttt caactttaat tttcatgttc ataaatgatt tgaaaccaaa
      301 gcaaatgcgt tgcacttttc ctccgtcttc cgaaaatcgt tcaattcact ggtaagtagt
      361 catttgatag tttgaggcaa attgttgatt gatgaagggt aggagctaaa aaaagatacc
      421 agttgaagag agaaaaagaa aaaaatagag ggtgttcttg aatgaaaaaa gttggaaaat
      481 tatttttcat ttccttttat tcctattaaa gtcagtgatg tcaatcatat taacaagact
      541 aaaaatgaaa ataaaaaaaa gattgttctt agtattgact cataaaaaat accactacta
      601 tacttacatt gtggttatag ccagttagga gagacatcga tgcaaaatac ctttcaatca
      661 aaatcacttt attatcagct ggtagaaata ttgaaacaac gaatcgaaaa cagtatggtc
      721 gctcatgaca agctgccatc agaaagagag ttgaccagtt tttatggcgt tagtcgcacg
      781 acggtgcgac tagcattgca agaactggaa aaaatgggct acatttatcg acaacatgga
      841 aaaggaacct ttgtttcgga attaaggaca aatgcgactg atctagctgg cgcatatagt
      901 tttaccgaac aaatgcgggc aatgggcaaa catcctgaaa ccaagatatt ggccttcaag
      961 aagattgaag caactaaata tttagcgagt catctgaatg ttcaactagg tgagccttta
     1021 tttcaattag accgattgcg cttagctgat gatttgccaa tgatgatcga gaaatcctat
     1081 ttgccagtga agctatttat gtccctttct caagaaattc tatttctgaa acctttatat
     1141 aatttgtttt cagagaattt caatcaaatc gtacggttgg ctgacgagga attttatgcc
     1201 agcattgtcc agaaagaaga tagtgagttg ctaaatattc cagaaggatc accagtgctc
     1261 catttagttc gctcaaccta taatctcgag aacgaagtga ttgaatttac attaagtgta
     1321 gcgcgggctg ataaatttca ttataagatc aggcatctgc gttccactaa ctgaattgga
     1381 ggaaagaaaa tgtttatgtt ctcaaaagaa gaactacgaa agctaggagc agaaaatact
     1441 acccgagaaa ttcgacagca accggaactg tggcatgaga cgtttgaccg ttatcaagaa
     1501 acagttgcag atttagatcg tttgtttacg gatgcgaaac aacatgctgg agagcgacca
     1561 atccgagtca tttttacagg ggccggaacc tcgcaatatg tgggagatac actaattccg
     1621 tttcttacta gtcaaggaaa tacgaaccag tttatttttc aaagtattgg ttcgaccgat
     1681 atcgttgcta cgccaaaaga atacctgatt cctgatgaga caacgatctt ggtctcattt
     1741 gcccgtagtg gaaatagtcc agaaagtcta gccgcagtta agatggccaa tcagctggtc
     1801 aacaagatct atcatatcgt gatcacctgt gcaccagacg gattattggc tacacaaatg
     1861 cctacaaaaa aagaaaatct agttttacta atgccaaaca aagccaatga cgctggtttt
     1921 gctatgacaa gcagtttcag ctgtatgacg ctgactgcgc tacttatttt tgatcaagca
     1981 agcaatcagc aaaaagaaag atatgttcat caattaagcc gactaggaga agaagtcatc
     2041 caacgagaat cagagatcca agggtatctg gcatcagact ttcatcgcat catttactta
     2101 ggttcgggaa gcttaggcgg attgactaga gaagcgcaat taaaagttct cgagttaact
     2161 gctggaaaaa tcgcaaccgt atttgataca tctttaggat tcagacatgg tcccaaatct
     2221 tttgtcaatg agcaaacatt ggttttcctg tttgtcagca atgactcata tacgcagaaa
     2281 tacgatgtag acatgctgga agaaatcaaa aatgacggta ttgcccaacg cgttgttgga
     2341 attggtcaaa agggttcatt gtttagtggt gattcctttg aattagttgc agattcgtta
     2401 ctgccggatg gctatcttgc tttagcagat atcatggtag cccaaacgat cgcattactt
     2461 tgttcggtga aggttggtaa tacaccagat acaccatcgc caagtggaac agtcaatcgt
     2521 gtggtcaaag gcgtaacgat ttatccatat gaaaaatagg aaagagtgtg aagcatgtta
     2581 gaatggtcgg aaaataaaag acaagcaatg gaacgtctat cgacgccaga aggcattatt
     2641 accgctttgg cgatcgatca gcgaggcgcg ctgaagaaaa tgatgtcggc tttagggatg
     2701 gaagcgacaa aggaaaaaat tgagcagttt aaaacactaa tttcccaaga attgactcct
     2761 tatgcctcag caattttatt ggatcctgaa tacggactgc ctgctgcaca gaagcgtgca
     2821 gcagcgagcg gtctgctgtt ggcatatgaa aaaactggtt atgatcaaac cgtacccggc
     2881 cgcttgccgg atctgctgtc ggactgttca gttctgcgat tgaaagagtt aggtgcggat
     2941 gcggtgaaat ttttactgta ttacgattat gaagaagcag aggaaatcaa tcggcaaaag
     3001 caagtttttg ttgaacgaat tggaagcgaa tgcatggctg aaggactgcc attttattta
     3061 gagttattgt catatgatgc taaaaatgta gatacgaaat cgctttccta tgccaaagca
     3121 aaaccgcgca aagtgatcga aatgatgaaa gaattctcga aaacccagta taaagtagat
     3181 gtcttaaagg ttgaagtacc agtaaacatg tcatttgttg aaggaaatgc aacaggggag
     3241 atcgcctata cacgtcaaga agctgccgct tattttaaag aacaaagcga tgcaacagat
     3301 ttgccgtata tcttcttaag tgctggggta tcagcggcat tattcatgga gacattgcgt
     3361 tttgccaaac atgctggggc acgattcaac ggtgtactct gtggcagagc aacttggaaa
     3421 gatgcagtcg ctcctttcgt tgaaaaagga gaagcggcaa ctttagaatg gctgacagaa
     3481 caagggacac aaaacattcg tcaattgaat gaagtgataa gagaaactgc catcccatgg
     3541 tatgagaaag taagtgaaag cacttgttaa tgtttctaat atattagtag tgataagaga
     3601 tatgtgtgat tataatttat cattattgaa actgggagga tattttatat gaataaaaga
     3661 ttgttagatt attcagcttc agattttttg aaagctaagc cgatggaatt aaagcaggca
     3721 attttggctt ctgaaggtag aacaattatg gcagaaaatg tgccaagtag ctgtccttac
     3781 atgggaaaat cagtgacaaa tgcagaattg gagagagctg ctggagctga tttaattttg
     3841 tttaatgcac tagatctttt taatcctcaa atttcaggtg tacctttaac tctaaaagag
     3901 aatccaataa tctggattaa aaaagcttta ggaagagcta ttggtgtaaa tttagaacct
     3961 gtagacaaag aagttttgat gagtgaaaat cgaactgaaa tttcaagtgg tagaatcgtt
     4021 tctcctgaga ctttaaaaaa ggcaaatgaa ttaggcttta attttatttg tttaactgga
     4081 aatccaggaa caggagtatc taatagaact attgagaaag caataacatt atccaaatca
     4141 atttttaatg gattaattat cgctggaaaa atgcatggtg ctggtgttga tgaaccaatt
     4201 atgaacttag atatagcaaa aagatttatt aaaacaggtg tagatatttt attagttcct
     4261 gctccataca ctgttccgta tttcatggaa agtgatttgc gaggaatcgt tgattatata
     4321 aaagatttta atagaaataa atctattgag gaaaaagttc tagtactaac ggctaatgga
     4381 acaagccaag actcttctga ccaagaaact attaaaaaaa ttgcattagc atcaaaagca
     4441 tgtggagctg atattcaaca cataggggat tctttaaatg gtatttgtct tccagaaaat
     4501 atttttgctt tgggtcaagc aattcgggga tatcgtcatc aaattattat gttaggaaaa
     4561 tctaattatc gttaattaag gaggaaatga aagatgatcg acaaagttca aaaaatagct
     4621 gcaaaaatta gtaatcaaag acatctacta gctttaagag acagcatggc tgcagttatg
     4681 ccattaatca ttattggttc cttttttatg ttattagcaa atttcccgat agaaggattt
     4741 acaaaatggt tatctgaaat tggagtacaa tcctttttga acaaagcatc agattcaaca
     4801 tttggtatta ttggattagc tgttacctac tctttgggac acaatctagc aaagacttat
     4861 ggtgtagact cattatcagc aggattatta tctttgagtg gatatgtttt acttacacct
     4921 ttattggatg cagatggttc tataggattt ccaacaaaat actttggtac ctcgggtctt
     4981 tttgttggta ttattgtagc acttgtatct acagaaattt tccggtggtt tgtacttaaa
     5041 aatattacaa ttaaaatgcc ggcaagcgtt ccaccaaatg taagccgagc tttcgttgct
     5101 attattcctg gcttttttgt cgttctacta tggtttattg ttgttgtaat ttgttataaa
     5161 cttggaattg aaaatgttca tgctcttatt gcagatacac tcgcaaaacc actatcttta
     5221 ctgacgaaaa ctttaccagg aattatattg gttatactta ttcaatgttt cttttggatg
     5281 tttggtattc atggtgcaca ggtaacgggg ccaataattg aaccgttatt attgcaaaac
     5341 tctgatgtaa atagaattgc ttatcaagct ggaaaagaac ttcctaacat tataacttat
     5401 gaattcttat ataactttgt tttttctggt ggtgcaggtt gtttatttgc acttgccatt
     5461 ttaacatttt tcttttcaaa gagtcaggaa aataaaactt taggtaagtt atcaatcgct
     5521 ccagtaagtt ttcaagtagc agagcccctt ctatttgggt ttccaactat attaaatata
     5581 aaaatgttaa ttccatttgt aactgcacca gtagtaacaa cattaattac ttatttttca
     5641 atgagtatgg gattagttgc aaaacctgta ggagcaacaa tcccatggac aactccaccg
     5701 ataattgccg gatttttagc ttctggtgga cgaataagtg gtgcagtgat tcaagtaata
     5761 acaattgcaa taaatgtttt gatatactat ccattcttta aattagatga taatgccaag
     5821 ctaaaaagcg aaaaaaatga ttagaactac ctatatgttt ctaactgtag tta
//
LOCUS       DACEMS010000132         5785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEMS010000132 DACEMS010000000
VERSION     DACEMS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.137"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..410)
                     /locus_tag="IW735_002394"
     CDS             complement(<1..410)
                     /locus_tag="IW735_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1045363.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHFVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEK"
     gene            complement(492..842)
                     /locus_tag="IW735_002395"
     CDS             complement(492..842)
                     /locus_tag="IW735_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045364.1"
                     /translation="MINNYLHYKAINWNVIEDELDNVVWERATSLFWLDTRVPIENDR
                     SKWANLQLQEQEQLNRLLILLTNIATYQSNELGEIIRDSARSQQEIAIINNFQFTEMV
                     HTKAYNRILSIRQF"
     gene            complement(891..1334)
                     /gene="nrdI"
                     /locus_tag="IW735_002396"
     CDS             complement(891..1334)
                     /gene="nrdI"
                     /locus_tag="IW735_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1045365.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            1481..2146
                     /locus_tag="IW735_002397"
     CDS             1481..2146
                     /locus_tag="IW735_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1045366.1"
                     /translation="MKNSGCLCTVPLFASLSLDEKNEIKCLLHHQTFKKGEIIFSPSS
                     EEQLLIISLGAMKIYRISRTGKEQLIRIVKEGDYDGENYLFGLPNDCLYGEAVEETKT
                     CTLYKSDFNKVMARYPQLSYRLLQLNACKTVAVEKQLQLLAFDRIEDRLALYLNQLSI
                     AAKMGEPITLPISMRELANYLATSPETISRKLRDFQERQLITRKGKQITLLEEFWFIF
                     NYI"
     gene            2136..2591
                     /locus_tag="IW735_002398"
     CDS             2136..2591
                     /locus_tag="IW735_002398"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAQ1045367.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            2741..4120
                     /locus_tag="IW735_002399"
     CDS             2741..4120
                     /locus_tag="IW735_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAQ1045368.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            4257..5099
                     /locus_tag="IW735_002400"
     CDS             4257..5099
                     /locus_tag="IW735_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1045369.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
     gene            5242..>5785
                     /locus_tag="IW735_002401"
     CDS             5242..>5785
                     /locus_tag="IW735_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1045370.1"
                     /translation="MAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTMEDPDFPRIAI
                     TYSLEENNQDSSAQQEEMKKIIEEYNQYFDTAWSLADIERYNGDINNCLARKRAEFKQ
                     FGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVT
                     FRKPATMEKNVEDATKLYSEA"
BASE COUNT         1854 a         1015 c         1110 g         1806 t
ORIGIN      
        1 cttttttctt tatgtccgtt tggttttctt ttccctttca ttggtaaatc gtgagggtca
       61 aaggttttat ctttaaacat ccgatatagt gtacgtaccg aacaagcaat aggaaactct
      121 gctcgaccaa taaggacatc tggagtccac ccttgagcca cctttttttg aatgtattct
      181 tgttgcccat ctggtaacac aatcggacgt cttccgcagt tacgtttgtt tttcttatat
      241 tgttcatagt agcttattgc gcttccacca gatttaagaa aacgatagac attataaatg
      301 gtttgtcgag aacgttgtac tagatgggct acttttgaaa caaagtgtga ttgattgaaa
      361 taagcttcta tcatcacgag ttcagttggt gtaagatggg tataggtcat ttgtactcac
      421 tccttataat tctttcgacg gaactatttt gagtgtagca caaatgactt ttttagttgt
      481 ctagcttaat tttagaattg gcgtatgctt aaaatgcggt tataggcttt tgtatgaacc
      541 atttcagtaa attgaaaatt atttatgatt gcaatctctt gttgggaacg tgcactatct
      601 cgaataattt ctcctaattc attggattga taggtagcga tattagtcaa aagaatcaac
      661 aagcgattga gctgctcttg ttcttgtaat tgtaaatttg cccattttga tcgatcattt
      721 tcgatgggga cacgtgtatc caaccaaaaa agtgaggttg ctctttccca tacaacgttg
      781 tctaattcat cctcaatgac attccagtta attgctttat agtgtaaata attatttatc
      841 atagtacctc ctaaaatatt gtttgaaagg ggatgatatg gtgtcatttt ttattgcatt
      901 gtttctgcaa ttctctctat atcttctgtc gtacctttta gttcaaattc atccaacacg
      961 gtaaaaccaa aatcatcggc gtattgatag gctgttaggc aaaattgttt attgaagttt
     1021 ctgttgccac taccaataat tcctaaacaa tagtctcggt ttttattgta agtaatcact
     1081 tggcgcaatg gtgttgtcaa aatttcgaca tctcctgttt caataccatt tccaccttca
     1141 agataagtag gcagaaatac aaaataggat tcattgatgt caaaagtgag tggttttcct
     1201 aatttgacta aatcttttac attcacataa tctaccttct tttgatattg tttttgacaa
     1261 tacgtatcta aatctgcgag aaaactcgtg gtatttccac ttaaactaat ataatagact
     1321 ttaattattt gcatgtatca cacctctttt taataaataa ccacatatat agtgcttgtt
     1381 ttaattagaa acgctatata tagtgtttat tgtacatgac atatcgttat agaaaattga
     1441 cgatggtcaa ttattttgat tttagaggag agaaaattac atgaaaaatt caggttgttt
     1501 atgcaccgtt ccactcttcg cctctctttc attagatgaa aaaaatgaga taaagtgttt
     1561 gcttcaccat cagacgttta aaaaaggaga aattattttt tcaccaagta gtgaagaaca
     1621 actgttgatt atatctttag gggctatgaa aatatatcga attagtagaa ctgggaagga
     1681 gcaattaatt cgtatcgtaa aagaagggga ttacgacggt gagaactatc tctttggtct
     1741 acccaatgac tgcctatatg gtgaagctgt tgaagaaacc aaaacttgca ctctctataa
     1801 atccgacttt aataaagtga tggcacgcta tccacagctc agttatcgat tgttacaatt
     1861 aaatgcttgc aaaacagtag ctgtggaaaa acagttgcag cttctagcgt ttgatcgtat
     1921 agaggaccgg ttagccttat atcttaacca actatctata gcagcaaaaa tgggggagcc
     1981 tattacttta cctatctcta tgagagagtt ggcaaactat ctagctacca gtcctgaaac
     2041 tatttctcgt aaattaagag attttcaaga aagacaattg attactagaa aaggaaagca
     2101 gattacacta ttagaagaat tttggtttat atttaattat atataaagaa gaagatatta
     2161 aaattgggat tgtcattgca cgattcaatg attttttaac taaaagctta ctaagtggtt
     2221 gtgaagatgg ccttacgcgt tcaggcgttt tgacagaaaa tatcgatgtt gcttgggtgc
     2281 cgggggccta tgaaatccca cttacagccg caacgatggc tgaaacaggt aaatatgatg
     2341 ccattattac cctaggtgcg gtaatcagag gagctacaag tcactttgat gtggtggtta
     2401 accaagcgtc aagtggggta tctcaagtag gcttaaccca taaattaccg gtcatctttg
     2461 gtatcttaac cgttgaaaac atcgaacaag ccattgaacg ctctggtact aaagctggta
     2521 accttgggtt tacttatgca acgaatacta ttgaaatgat taatttattc aaagaaatta
     2581 aacaacaata agtcatcatt ctatatgaaa atatctgtaa cagcataggg ctagctttaa
     2641 ttttcaatca tggtaagatg gaagatagga aaataaagcg cattcaatta attatcaagc
     2701 ctttctgatg atttgcgcaa aaagcttaag gagacttcat atgacgattc aatttaaaca
     2761 aacattagcg ccaaccaagg ataattctgg aaaaccaatc attttaagtg acgctacctt
     2821 cgctcaacgt aatgagaaag tccttcagtt aatgcgtgaa tatgacttct catcattgat
     2881 gatttatgca gataaagaac acggtagtaa ctttgaatat ttaactggtt ttattccacg
     2941 gtttgaagaa gctatccaag taatcaacca agacggaacg tcgacactga ttttaggtaa
     3001 tgagaattac aataaaacca gtatggcacg tacacaaagt gaaggtattc tttgcccatt
     3061 cttctcttta ccaaaccaac ctatggattt cactaaaact tttgctgact atcttaaaga
     3121 cgttaaagta gatgcttcaa aacgaattgg tttagtagac tggaagcttt tatctaatga
     3181 tatggacaac ttccacaacc atcattcaat accagcattt attgtagatg gtatacatga
     3241 agttttcggt tctgacaagg tagtgaatgc aacccaacta tacatgcatc caaaagaagg
     3301 tgctcgtgtg acaaacaacg cagaggaaat cgctcgttat gaatatggtg cttcccttgc
     3361 ttctgatgct gttcttgaag caatgaacca gctaaccgtt ggccaaagtg agttagcagc
     3421 aggtaatcac ttaaatcgcg atggtcaata tccatctgtt gtgaccattg cagcctttgg
     3481 tgatcgcttt gagggtgcta atatttatcc aactgataat acgctaaatg atggcgataa
     3541 agttgcatta actgtagcct ataaaggagg cttgtctagc cgctctggct atgcagtaaa
     3601 agaggaaagt cagctcgctg aacatgatcc aggatactta gaagaagtgg tttacccata
     3661 tttccaagcc tataaatggt ggctagataa tttaaccatt gggagaaagg gtggcgattt
     3721 ctaccaagca ttcagtgact tctatccgca agctacttat ggctggaatt tgtgcccagg
     3781 gcatttagta gctgacgaag aatggttaag ctcaccattt tatcaagact ctgatgcaac
     3841 aattcaaagt ggctacatct tccaactaga ctttattcct tcacaagcag gccacaatgg
     3901 tgtttcagct gaatcaacga ttgcggttgc ggacgcggca ctacgtgaac agattaaggc
     3961 tgaacaacct gaattatggg cacgtatctg taaccgaaag gattatctaa aagaagcatt
     4021 aggtttagaa ttatcagaac atttactacc cttagcgagt acctttgcct actatagacc
     4081 tttcttatta gacaaaaaaa ctgctatcta ctttggataa aaaaatgccc ctcaagattg
     4141 tttgccaaac ttgagggttt tttgtctatt tttaagtgaa taatgacgga ctatccaaac
     4201 ggaatcgcca taaagatgtt aatatatgta taatattata aagaaattgg aggacaatgc
     4261 ccttggacga gcagattgaa ttattattta ccatagatga aggctatttg aatcccttaa
     4321 aagtggcttt aacatcgatt cgtcagaata atccaggcca agctttccgc atttggttga
     4381 ttcacgaatc cattgcaatg catacgattc gcgaattgca gaagctaacg gattatctac
     4441 agtttggttt tgaagcgatt aaaattgatg gtagtcgatg gaatagcgct aaaactgaag
     4501 atagataccc aaaagagatg tatttccgcc tgcttgcggg tgaaatatta cctaaagaaa
     4561 tgaaacgcgt gatctattta gacccagata ttttagtggt taacccttta ttagatttgt
     4621 ggcaaacaga tttagaaggt tatatgctag cagctgctac acatgttgga ttgacggacg
     4681 tttctacacg agtaaaccaa atgcgacttg acgttgacca tgcttattac aattctggtg
     4741 tgatggtgat tgatttagat aaagctagag aaattattaa atggtcagat attgcacaaa
     4801 tgattgaaaa atataacttg ctattaatcc tacccgacca ggatatcctc aaccgattat
     4861 atggtaaata cactaaggaa attccagaag aaatttggaa ttatgatacg cgtaaatata
     4921 tgcggtattt caccaaaagt ctcgctcaac atgatattca ctgggtgatg gcgcatactg
     4981 ctattctcca tttctgtggt ggcccaaaac catgggacga taaacatgat aatcgtttta
     5041 ccagtcttta tcttacctat caaaatcaat taaagcgtat tgaagatttg atgaagtaaa
     5101 gtgaaacaag tgtttttcat tacctacggt ggtttaatgc ttatttatta tcgttaaatc
     5161 atgcttatac actttacaaa atttgatttt cgaaatggcc gtccaacgaa gtctgcgatc
     5221 ttaaccacga gttccattga catggccaaa aagtattatc gagcgatcaa agaaatgacg
     5281 aaagaacctg actggttaac aaaagagttt cctaatcagc ccattcgtga aggaagaacg
     5341 atggaggatc ctgattttcc acgaatcgct attacctatt cattagaaga aaataaccaa
     5401 gattcaagtg cccaacaaga agaaatgaaa aaaattattg aggaatataa tcagtatttt
     5461 gatactgcct ggtcattagc agacattgag cgttataacg gagacatcaa taattgtttg
     5521 gcacgaaaaa gagcggaatt caaacaattt gggaaacaaa tcgatttagt aatcgtagtg
     5581 gatcgtctat tgacaggttt tgacgcacct acgattcaaa ccctatttgt ggatcgtaat
     5641 ctagagtatg caggattgat tcaagctttt tcacgtacga accgtacgta tcctgaaaag
     5701 acaaaaggat tgattgtgac gtttcgtaag ccagcgacaa tggagaagaa tgtcgaggat
     5761 gcgacaaaac tgtattcaga agcaa
//
LOCUS       DACEMS010000133         5702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEMS010000133 DACEMS010000000
VERSION     DACEMS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.138"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1785
                     /locus_tag="IW735_002402"
     CDS             <1..1785
                     /locus_tag="IW735_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1045371.1"
                     /translation="NQYHYHVPKDKKKQFSVVKRQLQSADTIVIATDSDREGELIAWS
                     IIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYPKFEEVQARQIADWLIGM
                     NGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQN
                     GAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVETEKKSAASPRLFSLSSL
                     QSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLK
                     AEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTV
                     AMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGKESDGKEKEEEPLLPKVT
                     KGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEAT
                     RANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGE
                     RKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKC
                     QEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            2131..2727
                     /locus_tag="IW735_002403"
     CDS             2131..2727
                     /locus_tag="IW735_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1045372.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            2740..3639
                     /locus_tag="IW735_002404"
     CDS             2740..3639
                     /locus_tag="IW735_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1045373.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            3642..3773
                     /gene="ssb"
                     /locus_tag="IW735_002405"
     CDS             3642..3773
                     /gene="ssb"
                     /locus_tag="IW735_002405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1045374.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            3795..4127
                     /gene="ssb"
                     /locus_tag="IW735_002406"
     CDS             3795..4127
                     /gene="ssb"
                     /locus_tag="IW735_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1045375.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            4172..4405
                     /locus_tag="IW735_002407"
     CDS             4172..4405
                     /locus_tag="IW735_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045376.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(4564..4686)
                     /locus_tag="IW735_002408"
     CDS             complement(4564..4686)
                     /locus_tag="IW735_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1045377.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            4876..5100
                     /locus_tag="IW735_002409"
     CDS             4876..5100
                     /locus_tag="IW735_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045378.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            5543..>5702
                     /locus_tag="IW735_002410"
     CDS             5543..>5702
                     /locus_tag="IW735_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1045379.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTL"
BASE COUNT         2030 a         1028 c         1192 g         1452 t
ORIGIN      
        1 aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt cgtcaaacga
       61 cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga aggggagctg
      121 attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt taaaagactt
      181 tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt acgagacgca
      241 gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga ttggttaatt
      301 gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat accaggaagt
      361 ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt gcaggaaaag
      421 attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat
      481 ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga agcatttgaa
      541 gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat ccttcaagtc
      601 gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag ccttcagtct
      661 aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg
      721 tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac ggaaggggaa
      781 tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc cgaagcgatt
      841 ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt ccaggaacat
      901 tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca actaagtcca
      961 ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag
     1021 tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt aaaggcaatc
     1081 gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag cgacggaaaa
     1141 gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt gacggtggat
     1201 ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg tacgttgatc
     1261 actgcaatga aaacagctgg gaaaaccgtg gatagtgaag aagcgcaatc catattgaaa
     1321 gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac cttgaaacaa
     1381 aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg gattttgctt
     1441 tgccaagccg tggaaaaaga gccgctgctt acgagcgccg aaatgaccgc caaatgggaa
     1501 agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa ccacttttct agccaatatc
     1561 caaaaatttg tctctcactt attggaagtc gtaccagggc aaatccaatc cactgatttt
     1621 ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta
     1681 ggaatttgtc caaaatgcca ggaacaagaa gtcttactct atcaaaaggt ggcagcttgt
     1741 acgtctgaag cctgtgattt taaaaggtca gaagggttca tttgaagcaa cgattgtttt
     1801 aaaagaagac ttcactacag gctttgaatt ttctgaaaag aaaaaaacga acttcaagaa
     1861 aagaacacgc cgcactgcaa aataatggag ggttaatctt atgcgtaata ccacacacaa
     1921 acaacaagaa atcgaaaatc aaaaagccaa aattcgttca gaacaggaaa aaatgcgtgc
     1981 cagcaaacga agactgcagc aattgaagaa ccagcagagg attcaaaaag aaatcgaatt
     2041 agatcgataa aatcttccta actattgtat acgtataaac ttatgttata ctgtttttat
     2101 aagtttatac gtatacaaag gagggagaaa atgacaaaag caatggtata caagctgcta
     2161 gaagaattta atggaacttt ggctttgaag gatgccaaaa aagcgggcat ttctcctgta
     2221 acgatcaaac gtatggtaga tcgaggggag ctggatcgag agtatccagg ttttttcacc
     2281 ttacctggtc aatttcctga tgaattgttt atggcgcaaa aaaaatatga acgcggcatt
     2341 atttcacata ttaccgcgtt agatttgtat gatttaacag atatgatccc tagacagatc
     2401 gatctgactg taccttacgg ttatcacgtt tctgaaaaag gattgaagga attcgcagtt
     2461 gagctacact atacaaaagc ggaatggtat gaattaggta agattgaaat aaaaagtcgc
     2521 tatgggaatc ctattatagc ttatgatcca gaaaaaacct tgtgtgacat ttggaatccc
     2581 tggtataacg ttgaggatga aatcaaagta aaagcaatca aaaactacat ggagtcggac
     2641 agaaaaaatc ttagaaaatt gaatgagtat cgacgaattt tgcctacaga taaaacgatg
     2701 cgctcttata ttatggcttt aaattaaagg agaatacaca tgacacctac gcaattaaaa
     2761 gcaaaagtga agaatatttc tagagaaaaa gaagttgatc cgcagcttgt tttaagacat
     2821 tttatgatgg aaaaattctt ggaaaaaatc tcagagtctc cttatcgtga aaattttgtg
     2881 ttaaaaggcg gcttcttaat tggctcaaag tatggcattg aaaatcgaac aactaaagac
     2941 attgatacga ctttacggga aatgaaagta accaaagaaa cattgacaac ggttctaaac
     3001 gatatttttt caacgccgac gaaagaaggc attcaatttg agattcaagg aatgaaagaa
     3061 acgagggaag ctgattatta tccagggttt agcttacgtg tactggcaca tttggaaaac
     3121 atgcggccag attttaaggt agatgttact acaggtgact cgatctatcc tgcgacgatc
     3181 actcacagtc ataaattgat gtttgaggat cgaacgattc ctttggaatc ctatcctaca
     3241 gaacaaatta ttgctgaaaa attaagtgca acctttgatt ttttaacaga taattcgaga
     3301 ggaaaagatt tttatgatct ctatactatt cctaaaatgg agaagatcga tgagaagacc
     3361 ttgaaagata gtgtgagaaa tacatttaca cgtcgaggaa aaactgatcc gttaaagata
     3421 tactatgaaa aggatatgga tgtactgaaa aatagtcacg agctaaaaga tacctggaag
     3481 aaatatcaaa aaagcaatcc ttatgctgcg tcgatcacgt acgatgagac aatggattcg
     3541 attagtaatt tgatggaaaa agtgattcac gtcgaagaaa aagagcgaaa actagcttat
     3601 caaagacacc aacagatgaa tagagaaatg gagagataaa gatgattaat aatgtgacat
     3661 tagtaggacg cttaaccaaa gatcctgatt tacgttatac cgcaagtggt actgcagtag
     3721 cgacttttac cctagccgtc aaccgaaatt tcacgaatca aaacggcaat tgagaagccg
     3781 attttattaa ctgcgtgatt tggcgcaaac cagcagaaac aatggcgaca ttagctaaaa
     3841 aaggtagctt gattggtgta gttgggcgga ttcaaacgcg gacttacgac aaccagcaag
     3901 gacaacgtgt atatgtgact gaagttgtag cagataactt tcaactatta gaaagtaaag
     3961 cagcgacaga gagccgtgcg cacgctgatc aatcgtccac cagtccaagc accgccacct
     4021 ttgaacaacg gaatacagcg acgcctaaca acaatggatt gaacgcttca cagaatccat
     4081 ttggtggaca atcaatcgat attagcgatg atgatctacc gttttagttt gagaaagaag
     4141 gagtaagcat ggcaaaaaaa ttagtctgac gatggatcta ttgatacccg taattgcatt
     4201 tatagacggg atttctttgg gcgtcactga aaatcctttg gtaaatagaa ttgcgaaagg
     4261 atcactattt cttgtattcg tgtatcttgt ctgttcttat cttttaaaaa gagcaggaaa
     4321 ggataattcc ttttggcgat tgactaaagt cacagtagca gcgacagctg ttatacttac
     4381 ggctattcta ccgctttata tgtagagtta acgggaaatc gttgaacttc ttgcgaaata
     4441 ttgctataat gaacatgaaa aagaggagtt gcgctaacaa cccctcatta acagcaccga
     4501 ttaaagaacg gtggcagaat attagttatt tggaaataac cgctgacctg ctaaagtaga
     4561 cggttatttc tttttgtcat ttttaagcat atttacaatc aaactaatca aagcaatggt
     4621 aaacatacca aaaccaagaa tggtttgaat tgtctcatat gcggacaaaa ggcttactcc
     4681 tttcatagat tttagcactt acattcataa gcaccaccac ctttccagaa gtcgccaccg
     4741 tcttcaaact ttactgtctt gactattata gcatatctaa aatcgaataa aaattctcaa
     4801 ttttagctgt ttttgctata attttagcga ggtgatcgtg atggaacaat atattggttt
     4861 actcgataaa ttagggtgat caagacacag ccattgcttg tgcgatttac attacgcacg
     4921 agtcatgaac caatcaattg tgtagtggcc aatatagaaa tagcggataa attgctgatt
     4981 ttggaagaag gcaagtatac tatttcagtg acaggtcact tcaataaacg aaagcaacta
     5041 gtcattgaat caatgcaaat tagaaaccct gatcatttta cgcgatcgat gagcatataa
     5101 aagaagagct gcagtcctaa atgaggattg tagctctttt tgttttacat agaaacagta
     5161 aaaatgaaaa ggcctgttta ggaaggcaca agcgccttct caattgaaag acctttctct
     5221 cactccaacc gccaccgccg ttcgagaaga aaggaagtga acgctacgcg ttcactaaga
     5281 taaagggtct tgcatagcaa gacaaaatag aaaaaatttc ctctcaccaa aaggttcgct
     5341 cctgttcgtt atctctttat tgatttgtca gaccggcaac aagttgcctt ttatcaccaa
     5401 aaagcgacaa ggacaagggt aaatggcacg ctgcgcgccc ttgtcttgtc gcttttcgtt
     5461 gattcattct ggccaacaaa tcaataaaga aagaactaac gaaaggaagt aaaacctttc
     5521 ctaagaggag gcagaatcaa tcatgcgaaa gaaatttgat tattggggtg ttcccttcag
     5581 cgaattattc ccaaactatc atgcaccaca tactgtcgaa tgcgattgtg gagaaagagc
     5641 gaaatgtatc aaaagctatt gtttgtatca atgtccaaca tgcgggaaaa aatatacctt
     5701 gt
//
LOCUS       DACEMS010000134         5513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEMS010000134 DACEMS010000000
VERSION     DACEMS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.140"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(72..542)
                     /locus_tag="IW735_002411"
     CDS             complement(72..542)
                     /locus_tag="IW735_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1045380.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IW735_002412"
     CDS             872..2512
                     /locus_tag="IW735_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1045381.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IW735_002413"
     CDS             2666..3988
                     /locus_tag="IW735_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1045382.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4487)
                     /locus_tag="IW735_002414"
     CDS             complement(4056..4487)
                     /locus_tag="IW735_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1045383.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4552..5388)
                     /locus_tag="IW735_002415"
     CDS             complement(4552..5388)
                     /locus_tag="IW735_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1045384.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1646 a         1099 c         1005 g         1763 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc tacgccagtc
     4201 atcaaagcct ttccgatgcc tcttttttca gcatatttag acacagcaag tgccataata
     4261 ttgaacattg gatcgctaaa aatcgattca taggactccg cttgtacata gccagctgct
     4321 tttttagaag taggatcttc ataaaccaag aaaaaatttt tctctggctc cttcagcagt
     4381 ttcttcattt gtctcaccgt aacggttaat ggtatatcat accctaattg atccgcattg
     4441 atttcacgta gtgcttctgc atcttctaaa cgcatttccc gtaacatcca aatcaccagc
     4501 caatttctga ttttttaaaa tgaatttttt tatcatagta tactcttttt ttcatttaga
     4561 acaatatgtt tcagtgattt tgacttgcgt tagtctagat tctcctaatg tttgtaagat
     4621 gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg acaattactt gaaaaataaa
     4681 ggtcactttt tttggagaca aaaccctgct aaatcttgct ccccacaaac ctccaataac
     4741 agcagcgggg ataacataaa acaacataga taaatcaaag cgcataaagc tacttgttaa
     4801 agcaatcgtt accaattttg ccagttgaga aaagaaaatc gtagatagag aatatagtgt
     4861 tgcttctttt attggcaatg caaacatcag catcaacaaa gaaacattga ctggtccacc
     4921 accaatacct aaaaaactag ctaaaaagcc cagaaccccc ccacatataa ggtaccaagt
     4981 ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg tagaagaacg caaaaaccaa
     5041 ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc ttctcgtttt caaataactg
     5101 taaaaaaact tcgaatacaa tgttgccgac tactcctcca acaacagctc cactagatac
     5161 ccaaccgaca atctgccaat tcagtgattt cccgcttgat acttgtcgca cagtcgaaac
     5221 aatggacatc gtgaataccg caactgttga gtaaaaagaa atccctgcaa cagaatgggc
     5281 gccgataaga tcaagaatcg gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc
     5341 aactgtgttc gccagaacga tcaccacaaa ataaatcaga ccaatcatct tgtatttcct
     5401 ttctcttgtt tatatctttt agcgattgtt actcttttct tttattatac agtaaaagaa
     5461 acaaacgagg cttcgaaagc ttttctactt tttaaggaaa tttagtgcta aac
//
LOCUS       DACEMS010000135         5464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEMS010000135 DACEMS010000000
VERSION     DACEMS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.141"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            132..1106
                     /locus_tag="IW735_002416"
     CDS             132..1106
                     /locus_tag="IW735_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1045385.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="IW735_002417"
     CDS             1088..1549
                     /locus_tag="IW735_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1045386.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="IW735_002418"
     CDS             1599..2063
                     /locus_tag="IW735_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1045387.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="IW735_002419"
     CDS             2077..2826
                     /locus_tag="IW735_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1045388.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="IW735_002420"
     CDS             2826..3656
                     /locus_tag="IW735_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1045389.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="IW735_002421"
     CDS             3656..4303
                     /locus_tag="IW735_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1045390.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="IW735_002422"
     CDS             complement(4401..5177)
                     /locus_tag="IW735_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1045391.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5259..5402
                     /locus_tag="IW735_002423"
                     /pseudo
     CDS             5259..5402
                     /locus_tag="IW735_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1874 a          738 c          972 g         1880 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 ggtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaatt cattctgttt taagtaaaaa tccttatgtt accactcatt
     5341 ctgaagggga tgagccttat cgctacttgg ttgtggaacc aagtgataag acaattttat
     5401 aaattcaata ttaattaaaa taaaaacaaa caaagtagat tattttactt tgtttgtttt
     5461 ttta
//
LOCUS       DACEMS010000136         5429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEMS010000136 DACEMS010000000
VERSION     DACEMS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.142"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(101..559)
                     /locus_tag="IW735_002424"
     CDS             complement(101..559)
                     /locus_tag="IW735_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1045392.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(572..1897)
                     /locus_tag="IW735_002425"
     CDS             complement(572..1897)
                     /locus_tag="IW735_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1045393.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1914..3326)
                     /locus_tag="IW735_002426"
     CDS             complement(1914..3326)
                     /locus_tag="IW735_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1045394.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3343..4107)
                     /locus_tag="IW735_002427"
     CDS             complement(3343..4107)
                     /locus_tag="IW735_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1045395.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4211..5086)
                     /locus_tag="IW735_002428"
     CDS             complement(4211..5086)
                     /locus_tag="IW735_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1045396.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5341..5425)
                     /locus_tag="IW735_002429"
     tRNA            complement(5341..5425)
                     /locus_tag="IW735_002429"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5389..5391),aa:Leu,seq:caa)
BASE COUNT         1656 a         1145 c          799 g         1829 t
ORIGIN      
        1 aatcaaaatg attaaatcta gtgattgaaa catatcattt caaaaaaatt ttgtaaattc
       61 acccataaat atcaccctat ttaacttttt tcatcgtaca ctaaaattca aaacaactat
      121 ctttaatctt tacttcttgg ttttgtttta ataaatttcc ttcaaaaatg cctggagaaa
      181 ctacaatgat caccgtatct ttttgtgctt gctccaactt cttcaaatct ataatagcta
      241 acagtgtccc ttcttttacc tcctggttac tttcaacccg tatctgaaac ggacttcctt
      301 cgagttcaac tgtatctaaa cccatatgga tcagtatttt tctgccagat tcactttcga
      361 tcgttatggc atgtcgggta ggaaatatat tatcgattat tcctttaact ggcgcatgga
      421 tatatccatc atgattcgtt atagcgtatc caactcctat catttttgaa gaaaagacct
      481 cgtcttttac tgctgttaat ggaataattt gaccactagc tacagctttt agaatgtttc
      541 ttgttttaaa gaaatgcact ttttatcacc tctactttaa tgctggccaa tattttttgt
      601 ttactttgat catttcatct agtatttttt ttgcgactct ggcatttggt acggtttgat
      661 tcaatgtaaa tgcttgtaaa gctttttgat aagaaccttc aaagtaagca tctactagta
      721 acttctctac tgcaacttgt gcctccatca atcctttatg aaaatcactg attggttttc
      781 ttagtgtaat tggctccaca ccagttgcac caacatatgc cggtatttca acaactgcat
      841 ctgaccgtag attcggaatc gccccttgat taggaacgat tagcataaag cgacgacgat
      901 catcattcaa caaggaaata gctatatcga cgatatattg tccatgctcc ccaaaattaa
      961 agttcaatac ttcttccaca ataccgcggc gtattttatc agccatttct ttggtatttt
     1021 tctctcgtcc atccatgacc atattagcac gtgtatactc tttgtcagta tgctcaacga
     1081 ccatatcagg atataagtaa tactctaagt aattattcgg gatattactt ggaaaatttt
     1141 tcgtgatgac tgacatcatt tgaaaggttt tttgccaagt tttatctcct atattaaaat
     1201 cggcgacttg catttctttt gttattagtt tttcaataat ctctggcata atttctcgtt
     1261 gcaattcttt atcataaatc gatgtatacc agccaaagtg gtttaaacca taatacgtaa
     1321 cgatccaatt cttacgatca taaccgtaat tcactgcaat cgtttcttca attgaaatag
     1381 tcatatcaca ggcattaatc atttttacat tcggatattt acgacgaaca gcttcagaaa
     1441 cgatcgactc tggatttgta taattcaaaa tccatgcttt gggggcatag tcttgtatat
     1501 gatcgacaat ttccaataaa cctttgattg agcgcatccc ataagcaaat cctccgagtc
     1561 cacacgtttc ttgtcctact aatccatgtt taagtggaat cttttcgtcc ttttctcgca
     1621 tctccaatcc tccaacacga atttgtgaga aaacaaaatc gcatcctgta aaagcaagct
     1681 ttggatcatc cgttgcacga atgcgaatat ttgaaaaacc ttttcttttc agcataaatt
     1741 taacaatcaa gtacatatcc atatttcttt cttcatcaat atcatataaa cgtatttcag
     1801 ataatggcaa ccgtttttca ttgttcaaaa tggcttgaac gattcctggt gtatacgtac
     1861 tcccgcctcc agctatagtg ataatctgtt ttttcatcat catcttttcc tttctattct
     1921 tctaataatt tattgaattc tgccataaca taagtcactt ctaagccgat gataatttga
     1981 taattttttt cagttggttt aatgatcccg cttatcccta attgcttgat tcgctgttcc
     2041 tcgaccatgg tgggatccac tacctctacc cgtaggcgtg tattacaact atagacatcc
     2101 aggatattgg cttttcctcc aaacgcttgt agcatttttg ttgccatata ttcataattt
     2161 ttacttgcta accctaactc tgtttcttct aaggttactt cattgccaaa ctctgcctta
     2221 gcttcacgtc ctaatgtctg aatgtttctc ttattgatca aataataaaa agtaacgtaa
     2281 tacaaggcaa aaaagaaaat tccgattgga agaatcaaaa tagcattggt agcgattcga
     2341 aaattgatga gataatcaac aatacatgct ccgaatgtaa aaccaattcg tatattgaat
     2401 aagtaacaga caatccctgc taaaccggca aaaagtgcat ggatcacgta caacatagga
     2461 gaaagaaaca tgaaactaaa ttcaacaggc tcagtgatat ttgcaataaa tgaagttcct
     2521 gcaccgcttc ccatgattcc tttgaccatc tctttatttt ctttataagc tgctttatag
     2581 atagctagac atatagctgg tataccaaac atcattacta caaaaaaaac tgataaaaac
     2641 agtccagcct gaggatctcc gttgataaag cgtgtcatct ctcctctgaa aacttccttg
     2701 ctaggagatg tatagcttcc taaatcgtaa taaatatacg tattcaacac atggtgcagt
     2761 ccaaagggga tcagcaaacg attcaaaaac ataaatatcc ccacacctaa tgctcccata
     2821 tcaacgagca gttttgcaaa attatcaatc ccaatttgga tgagcggcca gataaagccc
     2881 aaaaaaacag ctgcgattgt ttgtagaatc atcaccatag tcaatgggaa tttttcgccc
     2941 gcaaaataag aaaaaactac tggtaatttt tgttctttaa agcgattata cgtccatgcc
     3001 gctactaaac cggaaataat tccaccaaaa atccccatat tcacttctgg gtccataatt
     3061 tctaagcctt ttttcaaggt agcaatcgct aaaaaaccag taagtgcagg tattcctttg
     3121 tccttatttt tgacaaaacc gattgcaact cctatagcaa ataagatatc caagtttcca
     3181 aaaacggcat ttccagctgc accaattact gggatgttca gcatatcgtc agctgataaa
     3241 cgcatgatca atcccgctaa tggcatcgct gcaactggaa gtagcatcgc tcttccaagc
     3301 ttctgcacat ttgatttaac attcattttt attcctcctc tattaaatat cctcatatgt
     3361 tactaactca atcaaaggat catttttcac tttttgagca aatgctgaat gaattaaaaa
     3421 ttcgacttgg tgggtacgag gatacacaaa acttgaacca tttaacaatt cctgatccaa
     3481 atacgctgcg tgacacatga cttcgatcgt tttatcttct ctcaatatca tgagtaaact
     3541 tgccaaatat tcttccacac tcgtattgat ttgtcgtttt ttttcatcag tttctcctac
     3601 gacatcgaaa caaggctcaa aataatcgac atgacgaact tcatcttttc gttcaaagtt
     3661 gctccgaacc ggcaaatcat atttttttgc taatgtaaca actacctcct gattttcatc
     3721 caaagtatga gtatgatgat gactgtctaa atgagttggt atgatccccg catgataaat
     3781 tttttgaatc tgagcattcc attcctgata cagttgatct ttgtcaatgt taaatggatg
     3841 ctgataataa gataaggatc tgaactctcc gctttcgata agagtatcga cagttttgag
     3901 gagtggtttg ccacacgtca atgttaaatg gactcctaca cctagcctag gcatttcctt
     3961 tcttagtttc acagcatgct cgaaacccgg catatttgcc atcaatgtag ttgatgtcaa
     4021 aattcctcgc tgataagcat ccataatccc ataattcaca ccatggctat agccaaaatc
     4081 atccgaatta aaaatcactt ttcccatttc attctccttc ttttttctac taaaataggc
     4141 gcaaccaaat aatcatagtc catttactat gtgtcatttg gttgcgccta tagatagtta
     4201 cgcccttatt ttaatagatt cttgctagat gaattgccaa atagatgatt tcattttctt
     4261 gaatggcaag ttgaaattgc tgttcgatga tatctttaat ctttagtgct gtacaatacg
     4321 ctttgctatg tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc gtttcttcct
     4381 gataaatcaa gcgtttaacc aggaatttga tatgaaccat taagcgatga taagtcagtg
     4441 attctttatc ataaacgttt gaataggatt tttctagatg ggacaaaatc gtggtcgtca
     4501 tttctactat ttttaagctc tttaaactat taggctctcc tagctcagca ttaatgatat
     4561 gcatagtgat gaatcctact tcatttgacc ctaactcggt ttgatatcga tgattgattc
     4621 gctcgaccac ttctactgct atatcatatt catttggata aaggaatctc atttctggca
     4681 acataattgc tgtgacatct ctatcttctc tttttcgttg aatagcaaaa taaatatgat
     4741 ctgtcaatgt aatatagata tttgaatcta atggagagga gatcctagct gttatcagtt
     4801 caatcacttc ttccgcaaaa tccataaatt cgattggaat ctgcgagact atctcactga
     4861 atcgtttgat cttttcttta gaatctaata caaataattt gtcagcatcc tcaacatcaa
     4921 atggcttact cactttagca gaaaagccaa tccctttccc catcgcaatt ttttctattc
     4981 catcgtcatc gatcaataca gcattatgat tcagcttttt catcacatac accgttaatt
     5041 cacctcctct atatgataaa acaaaacaac ttgcagactc cttcatacat atttttgtct
     5101 ccaaacaatt tttaaagaca aaaaacgacc taagaaagaa ccctttctta ggtcgtgcct
     5161 aattgaatag tagcacccta cataatttac aaaaatagtt taacacataa taaaagcgtt
     5221 atcaatatct tttttactcg ttttatgata atacttgtat cgaattgatt tggaggtaat
     5281 tacataaaaa caaaaaaaat atagcaactc tctataagaa agttgctata ttgataatta
     5341 taccggcggc cggggtcgaa ccggcacgtc ctcaacggac actggatttt gagtccagcg
     5401 cgtctgccaa ttccgccacg ccggcaaat
//
LOCUS       DACEMS010000137         5173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEMS010000137 DACEMS010000000
VERSION     DACEMS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.143"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1980
                     /locus_tag="IW735_002430"
     CDS             <1..1980
                     /locus_tag="IW735_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1045397.1"
                     /translation="THYHYHVPKDKKKQFKVVKQQLQSADTIIIATDSDREGELIAWT
                     IIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYPKFEEAQARQIADWLIGM
                     NGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQN
                     GAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVETEKKSAASPRLFSLSSL
                     QSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLK
                     AEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTV
                     AMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVT
                     KGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEAT
                     RANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGE
                     QKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKC
                     REQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKG
                     SFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            2221..2814
                     /locus_tag="IW735_002431"
     CDS             2221..2814
                     /locus_tag="IW735_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ1045398.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            2827..3726
                     /locus_tag="IW735_002432"
     CDS             2827..3726
                     /locus_tag="IW735_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1045399.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            3729..4202
                     /gene="ssb"
                     /locus_tag="IW735_002433"
     CDS             3729..4202
                     /gene="ssb"
                     /locus_tag="IW735_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1045400.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            4568..4828
                     /locus_tag="IW735_002434"
     CDS             4568..4828
                     /locus_tag="IW735_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045401.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
BASE COUNT         1920 a          917 c         1055 g         1281 t
ORIGIN      
        1 acacattatc actatcatgt tcctaaagat aagaaaaagc aattcaaggt ggtgaaacag
       61 caacttcaat cggccgacac cattattatt gcgacagata gcgatcgtga aggggaactg
      121 atagcttgga cgatcattca acaagcaggc gccgatcatg gaaagatatt taaaagactt
      181 tggatcaact ccttagaaaa agaagccatt tatcagggat tccagcagtt acgagatgca
      241 gaagagacct acccaaaatt tgaagaagca caagcacgcc agatcgccga ttggttgatc
      301 ggcatgaacg gtagcccgct gtatagttta ctcttgcagc aaaaaggcat accaggaagt
      361 ttttccttag gcagagtcca aaccccaacg ctttacatga tttatcaatt acaggaaaag
      421 attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat
      481 ggcgcatttg atgcgaagct tgatccgaat gaaacccaag cgacgcaaga agcatttgaa
      541 gattatttaa aagaaaaggg ggttcaattg ggaaaacagc caggtacgat ccaccaagtt
      601 gaaaccgaga aaaaaagtgc ggcaagtcca cgtctttttt ctctatccag ccttcaatct
      661 aaaatgaatc agctcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg
      721 tatgaaggga aatatctaag ctacccacga acagataccc cttatattac agaaggggag
      781 tatgcgtatc tgttggatca cttagacgaa tacaaacact ttttaaaggc cgaagccatt
      841 cctactccta ttcatacacc gaatagtcga tatgtgaata ataagaaagt ccaggaacat
      901 tacgcgatca ttcctacaaa gacggtcatg acggcagcgg cttttgaaca attaagtcca
      961 ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag
     1021 tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagct aaagacagtc
     1081 ggaaaagtac cagtagatct aggttggaaa aagctgtttg gcaaagagag cgacagaaaa
     1141 gaaaaggaag aagaaccgct acttcctaaa gtgacaaaag gagaagcggt gacggtagat
     1201 ctacaagtcc ttgaaaaaga aacgaagcca ccacaacctt ataccgaagg tacgttgatc
     1261 acagcgatga aaacagctgg aaaaaccgtg gatagcgaag aagcacaatc catattgaaa
     1321 gaagtagaag gaatcggtac agaagcaaca cgagcaaata ttattgaaac tttgaaacaa
     1381 aaggaatata tcaaggtaga aaaaaataag cttgtggtga caaataaagg gattttactt
     1441 tgccaagccg tggaaaaaga gccgctgcta acaagtgccg aaatgaccgc caaatgggaa
     1501 agttacttat taaagattgg tgagcagaaa ggaacacaag ctactttctt agctaatatc
     1561 caaaaatttg tctctcactt gttggaagtc gtaccgggac aaatccagtc aacagatttt
     1621 ggatcaacgt tacaagaggt gaaagcagcc agtgaaaaac aggaagcaac gcgtcattta
     1681 ggtgtttgtc caaaatgtcg ggaacaagaa gtcttgcttt atcaaaatgt ggcggcatgt
     1741 acgtctgaag cctgtgattt taaattatgg acaacgattg ccaaaaagaa actcacagcc
     1801 acacaactga aagagatcat tcaaaacggc cgaacaagcc aatcagtcag aggactaaaa
     1861 ggtcagaaag gttcgtttga agcaacgatc gttttaaaag aagactttac aacaggcttt
     1921 gaattttctg aaaagaaaaa aacgaactat aagaaaagaa cacgccgcac tacaaaataa
     1981 tgaaggggtt atcctatgcg aaatacaaca cacaaacaac aagaaatcga aaatcaaaaa
     2041 gcaaaaattc gtttagaaca agagaaatta cgtgcaaata aaagaagact gcaacaatta
     2101 aagaaccagc agcgcattca aaaagaaatc gaattagatc gatagacgtt aatcttgtta
     2161 caaataaact actatgatat atttgatgta gtgtatttgt aacgaaggga ggggaagtaa
     2221 atgcaagata aactgaaaga attgttggct attcgcgatg gaaatttatc tatgaaggaa
     2281 gccaggaaat ttggaatcgc agcaaccact gtacaaagat tggtgaatca aggaaaattg
     2341 atcaaagttg atcgtggtta ttatgtagag gatggtcatg gaatagatga tttattttgg
     2401 cttcagcaac gattttccag gggaatcttc tctcatgaaa cgactttaga tatttaccag
     2461 ctttctacga atatgccgaa attcattcac ttaacttttc cccatggcta taacgttgat
     2521 cgttcaatta caaaagagca gcagttacaa tttcatttcg tgaagaaaga agtttatgaa
     2581 ctaggaaaag tagagggaac ttcctttcaa ggaaatccta ttactatgta tgataaagag
     2641 cgtacacttt gtgatatttg gaatccgaga tatgaatttg aatatgaaat gaaattagaa
     2701 gcattaaaag agtacatgga agacccattg agagatccaa gtaaattaag agattacatg
     2761 acaaaactac atgtagaaaa agaaatgaaa taccacatgc aaagcttata ttgatggagg
     2821 aaaaagatga caccgacaca atttgcagca aaaacaagaa atttagcaaa gagtacagga
     2881 ttaaatgcac agttagtgca acggcatttt atgatggatc gtctaattga acaaatttct
     2941 gaatcaaagt acaaaaatga cttcattttg aaaggcggct ttttattagg atcaaaatat
     3001 ggaatcgata gaaggacaac aattgatatc gatacgacat ttagaaatag caaattaact
     3061 gaagaaaaat tgaagagcat ttttaatgat ttaactagaa aacccacaaa agaaggcatt
     3121 gttttctcta ttcaaggaat gaaggaaaca agagaagccg atttttatcc tggatttcaa
     3181 attaaattgg tggctaattt agagaaagta cgtgtaccgt ttaaaatgga cgtgacaaca
     3241 ggagattcta tttatcctga agtagataca cactatcacc aattaatgtt tgaagataaa
     3301 aaagtggaga ttccagctta tccaacagaa caaattattg cagaaaagct aagtgctacg
     3361 ttcagctttg gcacagataa ttcgagagca aaagattact acgacttatt taccattccg
     3421 aaactagaaa atatcgatac gaaagagtta tacaaaagtg ttcgaaatac atttacaaaa
     3481 cgtggggata caaaaccgtt gtccttttat tatgaaagag aaatggataa gatcagagga
     3541 aatgactatt tggaaaagca gtgggaaaaa tatcgctcag ttaacacgtt tgcgaattcc
     3601 atttcattta cagatacagt caacgaaatt gatcacctaa tgaaaagtgt gatcaaaatt
     3661 gaaaatcaag aaagaaaacg gcagtttaaa agatcacaac aaatgaatca agaaatggag
     3721 agataaagat gattaataat gtgacattag taggacgctt aaccaaagat cccgatttac
     3781 gttatactgc aagtggcaca gcagtagcga cttttaccct agccgtcaac cgaaatttcg
     3841 cgaatcaaaa cggcaatcga gaagccgatt ttattaactg cgtgatttgg cgcaaaccag
     3901 cagaaacaat ggcgacatta gctaaaaaag gcggcttgat cggtgttgtc ggacggattc
     3961 aaacacgaac ttatgacaac cagcaaggac aacgtgtgta tgtgactgaa gttgtagcag
     4021 ataactttca actattagaa agtaaagcag cgacagagag ccgtgcgcac gctgatcaat
     4081 caaccaccag tccaagcccc actacctttg aacaacggga tacaaacaat ggattggacg
     4141 cttcacaaaa tccatttggt ggacaatcaa tcgatattag cgatgatgat ctaccattct
     4201 aagtagcatt ttctattggt tgcatttgct acggtttatg ttataattta gtcaacaaaa
     4261 agaattttca atctataacc aaaaaccact agctggcggg ctagtggttt tcttttacgt
     4321 ttctattata agaattaacg ggtaggctta ctgtctttct tggcaaattt acaatcaagc
     4381 catttgcaga tatagtgcgc ggctacttct gccatgattg caaaaacaaa atcaaaaaga
     4441 attttcaaac ctataacctc ctccccttgc cagattaggc cggggtagca gacagcaaga
     4501 ctattatagc ataattgaaa tattcaaatg attggatttt tgctataatt ttagcgaggt
     4561 gattgtgatg aacaaatatg ttggtttgct tgataaaatc cgagtaataa aaacgcaacc
     4621 gttattggtt cgctttactt tacaaacgat tcatgaatcg attaattgtg tagttgcaga
     4681 tattgaaatt atcgataaat tgctaattat ggatgatgga aaatacaata ttgccgtcac
     4741 aggtcacttc aataaacgaa accagctagt tattgctagc atgtatgtta gaaatcccga
     4801 tcagtttacg cgattgatgg gcatataaaa aaagaagagt tgcaatccta atgtaggact
     4861 gcagctcttt ttgttttaca tagaaacaag aaaaatgaaa aggccttttt aggaaggcgc
     4921 gagcgccttc tcaattgaaa aacctctctc actccaaccg ccaccgccgt tcgagaagaa
     4981 aggaagtgaa cgttgcacgt tcactaagat aaagggtctt gcaaagcaag acaaaataga
     5041 aaaaacttcc tctcaccaaa aggttcgttc ctgttcgtta tctctttatt gatttgccag
     5101 accggcaaca agttgccttt tatcaccaaa aagcgacaag gacaagggta aatggcacgc
     5161 tgcgcgccct tgt
//
LOCUS       DACEMS010000138         5021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEMS010000138 DACEMS010000000
VERSION     DACEMS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.144"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(207..1169)
                     /locus_tag="IW735_002435"
     CDS             complement(207..1169)
                     /locus_tag="IW735_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1045402.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IW735_002436"
     CDS             complement(1171..2610)
                     /locus_tag="IW735_002436"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1045403.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IW735_002437"
     CDS             2795..4741
                     /locus_tag="IW735_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1045404.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1630 a          837 c          813 g         1741 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa t
//
LOCUS       DACEMS010000139         4990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEMS010000139 DACEMS010000000
VERSION     DACEMS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.146"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(62..2212)
                     /locus_tag="IW735_002438"
                     /pseudo
     CDS             complement(62..2212)
                     /locus_tag="IW735_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2358..2933
                     /locus_tag="IW735_002439"
     CDS             2358..2933
                     /locus_tag="IW735_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1045405.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3147..3842
                     /gene="vanR"
                     /locus_tag="IW735_002440"
     CDS             3147..3842
                     /gene="vanR"
                     /locus_tag="IW735_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1045406.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3820..4974
                     /locus_tag="IW735_002441"
     CDS             3820..4974
                     /locus_tag="IW735_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1045407.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1562 a          937 c          965 g         1526 t
ORIGIN      
        1 agagctattg tagaaaatat agtttgaaat gcaattttct tctttcagct ttttatttcc
       61 cacttgtaaa tcggtagatg gtggctcctt ttccagttcc ataatgtgga tttgcatctg
      121 catgtagtga tgaaacacct agctgcattc tcataccatc tgacgaagat gttgtaccgt
      181 cgccccaata gaaaggcaat tgtaatttat gatgaaagtt tactaatatg gcttgggctt
      241 tattcatggc atcttcatac atgcgccatt gagatacatt ggctagttgc ttatatgtaa
      301 gtccgggtgt ggcttcggcc atcttgctca agccaatatt cattcccatt cctaaaaggg
      361 cagccatgat aatgattgtt tcttccttat ctggttttcg attattggaa gcatgagtga
      421 attgctcatg aaatcctgtt atatgggcca catccatgag taaatcagtt aattttattc
      481 ttggtagcat ctgataaagg cttgcactaa atttttttgc ttcttctgga acatcttttt
      541 ctaagcgtgc aagtgatagc tttccttttt caagagaaac cccatctaac ttattggaat
      601 tggcagctaa ccactttaac ctttcattaa agctgctggt tctctccgtt atataatctt
      661 cgaatgataa actaactgat aatctcgtat tccccttcga ttgattccat gtatcttccg
      721 aaaacaaata ttcctcaaaa tccctatatt gtctgctgcc aacaatggaa acatctcctg
      781 cccgaacatg ctcccgaagt tctgttaaaa cagccatttc atagtaatga cgattaattg
      841 ttgtaccatc atcctcgtat aaatgtcttt tccatcgttt tgaaataaaa tccacaggtg
      901 agtcatcagg cacttttcgc tttccagatt cgttcattcc tcggataatc tcaacagctt
      961 gtaaaagtgg ctcatttgcc tttgtagaat gaaattccaa tactcttaat agcgttggcg
     1021 tatattttct tagtgaataa aaccgttttt gcagtaagtc taaataatca tagtcggcag
     1081 gacgtgcaag ttcctgagcc tcttctactg aagagacaaa ggtattccat tcaataaccg
     1141 attctaaaac cttaaaaacg tctaattttt cctctcttgc tttaattaat gcttgtccga
     1201 tgttcgtaaa gtgtataact ttctcattta gctttttacc gttttgtttc tggatttcct
     1261 cttgagcctt acgacctttt gataacaaac taagtatttg cctatcatga atttcaaacg
     1321 ctttatccgt tagctcctga gtaagttgta ataaatagat ggttaatatc gaataacgtt
     1381 tattttcttg aaagtcacgg aatgcatacg gctcgtatct tgagcctaag cgagacagct
     1441 gcaacaggcg gttacggtgc aaatgactaa tttgcactgt ttctaaatcc attcctcgta
     1501 tgtattcgag tcgttctatt atttttagaa aagtttcggg tgaaggatga cccggtggct
     1561 cttttaacca acccaatatc gttttattgg attcggatgg atgctgcgag gtaataatcc
     1621 cttcaagctt ttctttttgc tcatttgtta gagatttact aaccgtatta aatagcttct
     1681 tttcagccat tgcccttgct tcccacacca ttctttcaag tgtagtgata gcaggcagta
     1741 taattttgtt ttttcttaga aaatctatgc attcatgcag tagatgaatg gcatcaccat
     1801 tttccaaagc taattgatga aggtacttaa atgtcattcg atattcactc agggtaaaag
     1861 ttacaaagtc gtattcactt cgaatttctt tcaaatgatc ccaaagtgta ttttcccttt
     1921 gaggataatg atcaagcgag gatggactaa caccaatctg tttcgatata tattgtatga
     1981 ccgaatctgg gatgcttttg atatgagtgt atggccaacc gggataccga agaacagcta
     2041 attgaacagc aaatcctaaa cggttttctt ccctccttcg cttattaact atttctaaat
     2101 cccgtttgga aaaagtgaag taggtcccca gtatccattc atcttcaggg atttgcataa
     2161 aagcctgtct ctgttccggt gtaagcaatt ctctacctct cgcaattttc attcagtatc
     2221 attccatttc tgtattttca atttattagt tcaattatat atcaatagag tgtactctat
     2281 tgatacaaat gtagtagact gataaaatca tagttaagag cgtctcataa gacttgtctc
     2341 aaaaatgagg tgatattttg cggaaaatcg gttatattcg tgtcagttcg actaaccaga
     2401 atccttcaag acaatttcag cagttgaacg agatcggaat ggatattata tatgaagaga
     2461 aagtttcagg agcaacaaag gatcgcgagc aacttcaaaa agtgttagac gatttacagg
     2521 aagatgacat catttatgtt acagacttaa ctcgaatcac tcgtagtaca caagatctat
     2581 ttgaattaat cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat
     2641 ggcttgattt atcagaagat aatccataca gccaattctt aattactgta atggctggtg
     2701 ttaaccaatt agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta
     2761 agaaagaagg aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga
     2821 attatgcggt aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta
     2881 ctaatgtatc tagggcttca ttatacagga aattatcaga agtgaataat tagccattct
     2941 gtattccgct aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc
     3001 ctcctagcga tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt
     3061 agtcatttat tatgtaaatg cttataaatt cggccctata atctgataaa ttattaaggg
     3121 caaacttatg tgaaagggtg ataactatga gcgataaaat acttattgtg gatgatgaac
     3181 atgaaattgc cgatttggtt gaattatact taaaaaacga gaattatacg gttttcaaat
     3241 actataccgc caaagaagca ttggaatgta tagacaagtc tgagattgac cttgccatat
     3301 tggacatcat gcttcccggc acaagcggcc ttactatctg tcaaaaaata agggacaagc
     3361 acacctatcc gattatcatg ctgaccggga aagatacaga ggtagataaa attacagggt
     3421 taacaatcgg cgcggatgat tatataacga agccctttcg cccactggag ttaattgctc
     3481 gggtaaaggc ccagttgcgc cgatacaaaa aattcagtgg agtaaaggag cagaacgaaa
     3541 atgttatcgt ccactccggc cttgtcatta atgttaacac ccatgagtgt tatctgaacg
     3601 agaagcagtt atcccttact cccaccgagt tttcaatact gcgaatcctc tgtgaaaaca
     3661 aggggaatgt ggttagctcc gagctgctat ttcatgagat atggggcgac gaatatttca
     3721 gcaagagcaa caacaccatc accgtgcata tccggcattt gcgcgaaaaa atgaacgaca
     3781 ccattgataa tccgaaatat ataaaaacgg tatggggggt tggttataaa attgaaaaat
     3841 aaaaaaaacg actattccaa actagaacga aaactttaca tgtatatcgt tgcaattgtt
     3901 gtggtagcaa ttgtattcgt gttgtatatt cgttcaatga tccgagggaa acttggggat
     3961 tggatcttaa gtattttgga aaacaaatat gacttaaatc acctggacgc gatgaaatta
     4021 tatcaatatt ccatacggaa caatatagat atctttattt atgtggcgat tgtcattagt
     4081 attcttattc tatgtcgcgt catgctttca aaattcgcaa aatactttga cgagataaat
     4141 accggcattg atgtacttat tcagaacgaa gataaacaaa ttgagctttc tgcggaaatg
     4201 gatgttatgg aacaaaagct caacacatta aaacggactc tggaaaagcg agagcaggat
     4261 gcaaagctgg ccgaacaaag aaaaaatgac gttgttatgt acttggcgca cgatattaaa
     4321 acgcccctta catccattat cggttatttg agcctgcttg acgaggctcc agacatgccg
     4381 gtagatcaaa aggcaaagta tgtgcatatc acgttggaca aagcgtatcg actcgaacag
     4441 ctaatcgacg agttttttga gattacacgg tataacctac aaacgataac gctaacaaaa
     4501 acgcacatag acctatacta tatgctggtg cagatgaccg atgaatttta tcctcagctt
     4561 tccgcacatg gaaaacaggc ggttattcac gcccccgagg atctgaccgt gtccggcgac
     4621 cctgataaac tcgcgagagt ctttaacaac attttgaaaa acgccgctgc atacagtgag
     4681 gataacagca tcattgacat taccgcgggc ctctccgggg atgtggtgtc aatcgaattc
     4741 aagaacactg gaagcatccc aaaagataag ctagctgcca tatttgaaaa gttctatagg
     4801 ctggacaatg ctcgttcttc cgatacgggt ggcgcgggac ttggattggc gattgcaaaa
     4861 gaaattattg ttcagcatgg agggcagatt tacgcggaaa gcaatgataa ctatacgacg
     4921 tttagggtag agcttccagc gatgccagac ttggttgata aaaggaggtc ctaagagatg
     4981 tatataattt
//
LOCUS       DACEMS010000140         4809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEMS010000140 DACEMS010000000
VERSION     DACEMS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.147"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="IW735_002442"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="IW735_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1045408.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3252..3770)
                     /locus_tag="IW735_002443"
     CDS             complement(3252..3770)
                     /locus_tag="IW735_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1045409.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1580 a          786 c          730 g         1713 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccactta
//
LOCUS       DACEMS010000141         4772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEMS010000141 DACEMS010000000
VERSION     DACEMS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.148"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..549)
                     /locus_tag="IW735_002444"
     CDS             complement(<1..549)
                     /locus_tag="IW735_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HAQ1045410.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAI"
     gene            654..1160
                     /locus_tag="IW735_002445"
     CDS             654..1160
                     /locus_tag="IW735_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045411.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
     gene            1147..1599
                     /locus_tag="IW735_002446"
     CDS             1147..1599
                     /locus_tag="IW735_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045412.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            1592..1990
                     /locus_tag="IW735_002447"
     CDS             1592..1990
                     /locus_tag="IW735_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045413.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            2302..2375
                     /locus_tag="IW735_002448"
     tRNA            2302..2375
                     /locus_tag="IW735_002448"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2336..2338,aa:Ile,seq:cat)
     gene            complement(2888..3190)
                     /locus_tag="IW735_002449"
     CDS             complement(2888..3190)
                     /locus_tag="IW735_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045414.1"
                     /translation="MDVINETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            3287..4474
                     /locus_tag="IW735_002450"
     CDS             3287..4474
                     /locus_tag="IW735_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1045415.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         1583 a          756 c          902 g         1531 t
ORIGIN      
        1 aatggcaata tagattgctt ctttggagac ggtttgccgt tttaaaggaa tataagtagc
       61 atccatgaaa atagcgacat acttatcatt tagagttcta actttaaatg catttacttc
      121 ttcagtcagc actttagtca tgttggacat ggtttggggc gtgtagtgat gcccatacat
      181 tttctcaatc aagtctgcga tttccgacat ggtaatacct tttcggaaga ggtgaatgac
      241 ggtctcctct aaggtatcat tcgtccgtct ataagcagga acagtctgtt gcttgaactc
      301 gccgttgcgg tcgcgcggaa tctggagatg aagttccccg tactcggtct tgaccgtacg
      361 gtcataggag ccgttacgag aattacccgt gttaaaacca atgcgatcgt acttttcgta
      421 atcgaggaaa gccgttaact ccgttttgag aagcgtattg acagcttttt ccaaatggac
      481 acggaataat tcattcaaat cgcctttgtt cgctagagtc tttagaattt ctgtagtaaa
      541 atcgttcata tggaagtcct cttttctgtg aatgtgttgt ggtaactcta ttctacagaa
      601 gggacttcct tttttctatt tacacaaaat attttacact ctcgtttagt gagatggaca
      661 ttaagtggtt ggttcaacaa aatgatagca atttagaatt ggcgattaaa tatctcgaag
      721 aaactatctt tgaagatgaa catcttacag ataatttttt acaagtatta aaatatcttg
      781 aaatttactc tgttaaaaaa aataaattaa ttggtgaaaa tgattctcca ataaaaacac
      841 ctattgagct aagcttaaga aatcggatgg gaatactcca aagatcggaa atagtaaaag
      901 aactttttta tcataaattt agttatgaaa taagactaga tgatacttat gagcattata
      961 gaattgtttt tttcgtatac aattctatag aagatgctac tgcgactact gcattaacat
     1021 tcggttttac gaaaaatgga acaatcaatt cggataaaac ccgtcaagct gctacagaaa
     1081 gtgatgatat atgtaagaaa gtatgcaatg gggaagagaa ttactggata ggggaggaaa
     1141 agttgaatga aatctattaa tgatttatta aaaaaacatc ctgatttata taaaaaatta
     1201 aattctgaac aaagaaacct gtccaaacaa ttacttaaaa taagattaaa aaaacaaaaa
     1261 aacgtgacag aattctcaaa aatgttagga ataagtgaaa aaaaatatct tgaatttgag
     1321 ttcggagata tgcatattcc tgttgcagag tacaataaac ttttgaaaca agttgaaaat
     1381 ttgtcctata aagattcaat gaatttcact attaaggttg ctggcggccc tattaaatat
     1441 gaccgtattg aaaataaacc ttatattcgg aataaaagtt actcagtatt taaaagtaga
     1501 aagagaaaag aagtatcgtc aaatatggtc tatgctagtg ttaattacca agatacgagc
     1561 cgctatattg tagaaaaaga ggtatatgta catggataaa ggaaatagtg ttacttttgc
     1621 acttgaaaat attagtttga ataaaattca ttttgaatta actgataatt ttagttatgg
     1681 agatttagag tttaatattg aacttggata taaagaagac gaagcagagt atctagaaaa
     1741 aaaacttgtt tctttacgag ctcacattgt aggggggatg gaaggagcac cggtagatat
     1801 tttactcttt tcagttattc gtgtttctac aaatgaacag ccatataaaa aattagtaga
     1861 agaatctatg ggagagctag ctaaacctat cctgaataaa gcttctaatt tgatttctaa
     1921 tttatatagc gaagtaggag ttatgcctac agttattgat ttatatcagc tatatagtca
     1981 acaaaaataa aatacaagta atagagttat ctatttaaat ttgcaagaca aagacctcaa
     2041 ccagtcatcc ttggttgagg cttttttgct ctgtaattaa ctcgtataga tcctgcaaga
     2101 tagtttgttt ctgaataaat gattgatatg aagtatttag atgactgtat tgacaactat
     2161 aaaaatcatt cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc
     2221 acactccaag ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat
     2281 ggctttatgt ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa
     2341 ccgtccggtc gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct
     2401 gtgttagaat tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa
     2461 tgagtggctt ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata
     2521 tttctgtata tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttaaat
     2581 tgtgagatga taataaagaa gagtttaaag cgcaccccaa gccacttccc cataagtgtg
     2641 ttacgcttta aactctttta tatttgaagc tattaaaagg cataccatat ttttgaaaaa
     2701 aagtgagaaa aaaggcttac aattggagtg gtagttaatt agtgacttat ttttgatttt
     2761 atagcactga tactataaaa tatagatatc atcatattac acaatcttaa tactaactta
     2821 aaaaatatct cctttcacaa gtatggtgat aaaattcgtt ccgggctacc tttttaggta
     2881 gcctacttta atctttgtat ctttctggat caacgaaagt atactttata tagtcataac
     2941 gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa aagtatacat
     3001 ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta gaatagtaga
     3061 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc catactttat
     3121 aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt gtctcattga
     3181 ttacgtccat ataattgtgt aaaagtgaag catcaaaaaa gccattgatc tcagttagga
     3241 gtacaatgtt ttttgcagaa aacatactcg aataggactg atatcaatga ctcaagtaca
     3301 ttttactttt gaaagcgaag aaattcaagc aattataaac gaaagcggtg cgaatgatac
     3361 cgcaaaaaca cttatgacta tcatgtttaa tcagctgatg gaagaacaac gaaatcaata
     3421 tattcaggca aatgcctatg aacgttcaga agaacggcaa tcacaaagaa atggttacta
     3481 cgatcgttcg tttacaactc gaattggtac gttagaactt catgttccac gaaccagaga
     3541 cggtaagttt agtcctacta tctttgaaag atatcagcga agcgaaaaag ctttgatcgc
     3601 tgccatgatt gaaatggtca tttctggtgt ctccactcgc aaagtaacta aaacagttga
     3661 actgctaacg gatggtgcga ctgtttctaa atcattcgtt tcaaatctga tgaaacagtt
     3721 ggatccattt gtttttgagt ggagaaaccg gagcctagaa ggctcggaat atccattttt
     3781 catgtgtgat gccctgtata tgaaagtaag ggaaaatcat cgtattgtct caaaaggtgt
     3841 atatattggt atcggcattg attctgatgg acgacgtacg attcttggtt ttgatgttca
     3901 agacggcgaa tcggaagata attgggatac cgtttttcaa tcgttcgttc aacgaggttt
     3961 gttcggtgta aaactggtca tttctgatgc acacaaaggg ttagttaagg ctgttcgcaa
     4021 gaacttttta ggtgcgagct ggcaaagatg ccaagcccac tttttgagaa atatttttga
     4081 taagctgcca aagaaagttt cttctgatgt aaaagacgag ttgaagagta tctttaaagc
     4141 ctctgaactt gaattgacac gtgaaagaaa agagcacttc ttggaaaaat atggttgtga
     4201 ttcaaaatta agtgctgctt gcgacatttt agaaaacggc tttgaagatg ctattcaaat
     4261 tttgtctttt cctgaaaata tccgccgaag gattcgtaca accaatgtgt tggaacggtt
     4321 gaacgaagag attcgccgga gagaacgagt gattcggatt tttccaaata tcaattccat
     4381 cactcggata attggaacac ttttgatgga aaaagacact gagtggctgg cttctccacg
     4441 aaaatattta gaatttaatt cgaataacat ttagcaccat gaacctgtgc gagtctcttc
     4501 tttctgagga gagacatcaa gagtcgctgc gctactgctc ctgacctctc tcctcagaaa
     4561 gaaaggcatg gcagttaggt tcacaacgca aaacattgtg cttttgatga ggttgaactt
     4621 ttacacaaga ttgtggactt gactgtctca ttaaaaagca taaccattac tccgatctgt
     4681 ttttaaaatt agtttctacc tctaatatat atcgagtttt tattttgcct tcagagaata
     4741 ctgtttcttt ttttgcagtt acaggttgtt ta
//
LOCUS       DACEMS010000142         4739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEMS010000142 DACEMS010000000
VERSION     DACEMS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.149"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..522)
                     /locus_tag="IW735_002451"
     CDS             complement(52..522)
                     /locus_tag="IW735_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1045416.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="IW735_002452"
     CDS             complement(681..1139)
                     /locus_tag="IW735_002452"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1045417.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="IW735_002453"
     CDS             complement(1310..2455)
                     /locus_tag="IW735_002453"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1045418.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="IW735_002454"
     CDS             complement(2468..3385)
                     /locus_tag="IW735_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1045419.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="IW735_002455"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="IW735_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1045420.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1485 a          854 c          887 g         1513 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aaccattagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaa
//
LOCUS       DACEMS010000143         4726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEMS010000143 DACEMS010000000
VERSION     DACEMS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.150"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="IW735_002456"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="IW735_002456"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1045421.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="IW735_002457"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="IW735_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1045422.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="IW735_002458"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="IW735_002458"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1045423.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          834 c         1010 g         1227 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagtt
//
LOCUS       DACEMS010000144         4714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEMS010000144 DACEMS010000000
VERSION     DACEMS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.151"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(213..443)
                     /locus_tag="IW735_002459"
     CDS             complement(213..443)
                     /locus_tag="IW735_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1045424.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="IW735_002460"
     CDS             complement(673..1491)
                     /locus_tag="IW735_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1045425.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="IW735_002461"
     CDS             1652..2341
                     /locus_tag="IW735_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1045426.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="IW735_002462"
     CDS             2355..3857
                     /locus_tag="IW735_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1045427.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="IW735_002463"
     CDS             3870..4346
                     /locus_tag="IW735_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1045428.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4443..4553)
                     /locus_tag="IW735_002464"
                     /pseudo
     CDS             complement(4443..4553)
                     /locus_tag="IW735_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1590 a          673 c          830 g         1621 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4501 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4561 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4621 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4681 tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DACEMS010000145         4582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEMS010000145 DACEMS010000000
VERSION     DACEMS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.152"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(348..1301)
                     /locus_tag="IW735_002465"
     CDS             complement(348..1301)
                     /locus_tag="IW735_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1045429.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1324..2079)
                     /locus_tag="IW735_002466"
     CDS             complement(1324..2079)
                     /locus_tag="IW735_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1045430.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2076..3035)
                     /locus_tag="IW735_002467"
     CDS             complement(2076..3035)
                     /locus_tag="IW735_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1045431.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3028..3978)
                     /locus_tag="IW735_002468"
     CDS             complement(3028..3978)
                     /locus_tag="IW735_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1045432.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4128..4580
                     /locus_tag="IW735_002469"
     CDS             4128..4580
                     /locus_tag="IW735_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1045433.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
BASE COUNT         1494 a          964 c          776 g         1348 t
ORIGIN      
        1 catcgtttaa agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag
       61 tttcgctttg tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat
      121 cacactattt tttgccactc cccattctag aatcaacccg tatgatgtag agaatgaatt
      181 cacgttgaaa aaagatttcc caaataaata aaaaccatcc gtaaaatcat caatacgatt
      241 ttacggatgg tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga
      301 aacattattg ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct
      361 tgattcacat cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag
      421 acatctggtt caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt
      481 tcattggcag aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact
      541 acaaataaga tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga
      601 gtggatgctt cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg
      661 aatctagaac cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct
      721 tttttgtcag tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct
      781 aattgagttt tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc
      841 tggaaagatt cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga
      901 cttagatctt tttgaaaatc tctttgccgg ccagaaatga tgatcaggtc tggctgaagc
      961 tgattgattt tttctaagtc tggttctttg atcccacctg ctgattccac ttttttatag
     1021 gatgaaagat actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca
     1081 agagcatcca tcgtatctaa agaaccattg tcgaatacga cgactttttc aggattcttt
     1141 ggcacctcga tttgatcgcc attactgtct tttatcgtta atgccgtttc agcttgcgta
     1201 ctgcttttct ttgtatctgc ctgactggtg ttgtcactat ttccacatgc tgctaaacca
     1261 aatgatgcta gaatcaagat acctactact aattttttca tctcttctct cctaatgtta
     1321 gttttagaaa taaagacaga atctgcgacc ttcaatctca caaattcgta tgtccatttc
     1381 gtacaattcg tccagtacat ctttttttat catttcttgg gtcgttcctt gcgtgaataa
     1441 ctgcccatct ttcattgcaa taatttcgtc tgcataactt gcagcaaagt tgatatcatg
     1501 aagaacaata acaattgttt tcccgatttc ttgtactaat ttttttaaga tctgcatgat
     1561 ttgaaccgca aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt
     1621 gtcttgcgct agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc
     1681 aatcgattga tccgctattt cttctaaccc aaggtgttcc agtgattcct gaacgatttc
     1741 ctcgtctttt gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc
     1801 tttcacggtg atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc
     1861 taattcattg gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc
     1921 ttttggtatc aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc
     1981 gataaaagca gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt
     2041 tccatggtac agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga
     2101 atcaattttg tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta
     2161 ttccagccga aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc
     2221 agaaagcttc cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata
     2281 tttgcgacaa taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc
     2341 aaaagactga cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt
     2401 actgcttgtt catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg
     2461 taccagcagc cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca
     2521 aagcttgcaa ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt
     2581 aaaaaggtac tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa
     2641 aaaagattcg ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc
     2701 atcagcccaa cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct
     2761 ccgataaaga agaaaaggac cgtttggaca aggacatata acgactcaag tcccaatact
     2821 gaaggagtca aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca
     2881 gctagcgaga caaggataaa tgcaaaaatt ttcttactcc tcaatgccca tgcaaaatgc
     2941 cagtttccat atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc
     3001 ataccaataa aaatagatgg ctttttttta agcatatcgt cgccccctta tcagaagata
     3061 aaggaagata gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa
     3121 aatcaacacg cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt
     3181 tacgccaatt gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat
     3241 cactccgata aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac
     3301 taagccaatg cctaaaaact gcatcttctg gtaatctatt cccaaattta ctgcaaacga
     3361 ttcgcccaga ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg
     3421 aacaacagca tacaataact cataatttcc tcgtatcaca gtcgcaaaat ttccttgaag
     3481 ccatgaagtc atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc
     3541 aatgatattc cctaacatga ctccaatcaa cggaagcatc aattgatttt tctgggggaa
     3601 caatcgcgta agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa
     3661 ggatcggaca atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg
     3721 cgcactatcc atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg
     3781 catgatcaac ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg
     3841 caaacgagtc actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa
     3901 ttgtacattc gatactccga tgaacaatga aatactactt aaaataacta ataatgctac
     3961 tagtcgtccg tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt
     4021 taagtgaatc gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat
     4081 atgctataat cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg
     4141 atgtaaaaag ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac
     4201 agcataaata tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa
     4261 tgatccaacc aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt
     4321 ccatactctt gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa
     4381 cgaaaaacaa tagccgattt actgtagtca acgattacgt cactgcaaat cctgaggaat
     4441 tcggattgct cttaacagca aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa
     4501 aatacccaga agaaaactct tcgccagttg aaaaagaaaa accaaaaaag aaaaaaagct
     4561 tctgggatcg tttattttaa aa
//
LOCUS       DACEMS010000146         4523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEMS010000146 DACEMS010000000
VERSION     DACEMS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.153"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            176..442
                     /locus_tag="IW735_002470"
     CDS             176..442
                     /locus_tag="IW735_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1045434.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            586..2577
                     /locus_tag="IW735_002471"
     CDS             586..2577
                     /locus_tag="IW735_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1045435.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            2931..4232
                     /locus_tag="IW735_002472"
     CDS             2931..4232
                     /locus_tag="IW735_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1045436.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         1364 a          797 c          998 g         1364 t
ORIGIN      
        1 cgctgatttc acaacctctg attcacggtg ttccagaagc agcttctacg gaaataagct
       61 gaacactttt gttacaactt ctttttttct ggagtttctt ggagcatatt tttggagtcc
      121 ttctgaataa agcgtaaaat aaaagtgaga aatatggata aaggagggaa aggagatgct
      181 gaatttttta tggtcgttat ttatcggcgg tatattaggt ttgatagctg gcgcaatttt
      241 aggaaaggaa gtcccgggag gcgtatctgg aaatatcatt atcggttttt taggtagttg
      301 gctcggcgaa tttttactag gaccactggg accggaactc ggcggttttt atttgatacc
      361 agcattattg ggtgcaattt tttgccttgc tctttattca tttgtggcaa gacgtatcag
      421 agaacgtcca aagaaagaat aattttattc aaataagttg acacttcagt gtgttacttg
      481 gataattaga agcaatttag gaaagcgggt gtcatggata agaatatcat ctatcattca
      541 agcttaccta aaggatatcc atataacaag aaatgaggga aaaacgtggc aacaacgcac
      601 aataccaaaa agatatcaat ggctggcctg cttgtagcga tgggagtcgt ctacggagac
      661 atcgggacaa gtccgcttta cgttatgaaa tcgattgttg aagataatgg cggattgtca
      721 ggaatcaatc ctgattttgt tatcggatca gtctcattga ttttttggac attgactcta
      781 ctgacaacaa tcaaatatgt cgtgattgca ttgaatgcag ataaccatgg tgaaggtgga
      841 attttctcac tctacacact tgtccgaaaa ggtgggaaat atttgatcat tcctgcaatg
      901 atcggaggag ctgccttact agcagatggg gtattgaccc ctgctgtgac ggtaacgact
      961 gcgattgaag gcttgcgagg aattccgcag ttttttgacc ggttcggtaa taaccagaac
     1021 gtgatcgtca ttatcacttt agttattatt tttgttttat tcatggtcca acgctttggt
     1081 acagatattg tcggaaaagc attcggacca atcatgttcg cttggtttac ttttttaggc
     1141 gtaatgggat taatgaactt cagtcaagac tggacagtta tccgtgcact gaatccttat
     1201 tatgctatcc agctgctttt tagtgcagat aacaaattag gcttgttcat tcttggaaat
     1261 attttcctgg ctacgacagg tgcagaggcg ctttattccg atcttggaca tgtcggcaaa
     1321 cgaaatattc gtctgagctg gccttacgtc aaaatttgtt tggttatcaa ttattttggg
     1381 caggctgcgt ggttgttgaa tgtttatcaa aaccctgaag cacagcaaat caagaacctg
     1441 aatccattct tccaaatgat gcctcaaagt tggatcgtga tcggcgttgc ttttgcaaca
     1501 gtcgctgcag tcattgcttc gcaagcattg attacaggtt cgtttacgct agtttcagaa
     1561 gcaatcaagt tgaaactgct gcctcggtta aagatcatgt atcctgggaa cagtatcggg
     1621 caaatgtata tcccagcagt caatctgatt ttatggatcg cctgttctct agtggttgtc
     1681 acattccgaa cgtctcatca tatggaagct gcttacggtc tatcgatcac tgtgacgatg
     1741 ttgatgacaa cagcactgct ttacttttat cttatccaaa atgggcattc tcgttggctt
     1801 gcatatttag ttaccttttt cttcggtgcg atagaagtcg ttttctttat ctcaagtgtt
     1861 gtcaaattct tccgaggcgg ttttgtagcc gttctgattg gtttgatcat cttagtcgtg
     1921 atgtttattt gggaacaagg gaacattatt cgcgaatcgt caaccgagga tgtgtctttg
     1981 agagattata tcccgcagct tagagaatta cgtgatgata cttctttacc aatctatcag
     2041 acaaatgtgg tcttcttggt tcctgatatg gctgaagata aagttggccg acagttcatc
     2101 tattcgattt tagataaacg gcctaaaaga gcacgagtct attggtttgt tcatgtggaa
     2161 gtgacggatg agccatatac gaaagaatat caagtagata tgatggacac tgattttatt
     2221 gtccaagtga atctgttctt aggcttcagg gtccaacagg aaatcaacgt atatgttcgc
     2281 caagtcatcc atgatttgat gaaacaaggc cgtttgccaa aacagccaca aagatactct
     2341 ctgacaccag ggcgagaagt tggcgatttc caatttgtcc tgattgatga agtagtttcc
     2401 aatgtgacaa ctctaggaaa atggcaacgt caaatcatgc aggcaaaact ggcaatcaaa
     2461 aaaatcgcta cctcgccaga aacatggttt gggctagagt acagcgaagt aaaacatgaa
     2521 actgttccgc ttatcattgg aaatactcgg aaaacatggt tgaaagagcg gttgtgaaag
     2581 tagcgctttg acctgaacta atgtcgaatg tcagctactt gaacaccgtt tatcagaggt
     2641 catgaaaaag ctgtactgca acaaacagta agaacgagca gataaaaaca gcttctcatg
     2701 tttttcatct gtttgggctt aatgccgcag atgcaagctt ttgaataccg tttttatcag
     2761 aggtcatgaa aaagctgttt agcaacaagt aacgcaatga ttttgaagtg ataaggagat
     2821 gtggctgaaa aatgccgcat ctcctttttt attcctaaat atgaatacgc attattttca
     2881 atctttttgt agaaaaaggg tacaatagaa aaagagaaag gcgggagaat atggttatca
     2941 atttcattat tttatttttg atgattgcaa ttacttcatt ttttgtagca agtgaatttg
     3001 ctttagtgaa aatcagacga tcaagattgg aacaactaga aaaggaaaat gtaaagaacg
     3061 ccaagctagc tcttcatgtt acacaccatt tagataacta tttatcagct agtcaattag
     3121 gaattacgct aaccggtttg atcatcgggt gggtcggtga aggatctgtc gcggctttac
     3181 tagaaccagt gatcggtaaa ctacctttta gttcggctat tagcagtaca atctctgtgg
     3241 cgctgggatt tattttagtt acgtatatcg atgtggttat tggcgaactg cttcctaaga
     3301 gctatagtat cgtcaatacg gagaaggtcg tcttgttcgt agttaaacca cttcattatt
     3361 tttataaagt aatgtttcct tttatttggg tattgaatca ttcagctgca gggctaggga
     3421 agctattagg tgtgcgtctc gtatctgaag gagaagagac attgtctcaa gaagagctga
     3481 cattagtcgc attgaattct tatgaaaaag gagagttgac caaagaagaa tatcattatt
     3541 tggaaaacgt gtttgaattt gatgatacct tggcaaaaga tatacaagta gaccgtacgt
     3601 ccatgcaagt attcgaagct tcagaaaccg tcaaagaagc gataaaagct tcgattgaac
     3661 aaggacatac tcgctatccg gttatcttag aatcaaaaga caatgtttta ggttatgtca
     3721 ccttgccaga tttgatcaag caatcttata aagatgacca gctgacagta gaacagttga
     3781 ttgaagagcc gatcgtaaca actgaaacga tccctataaa aaaattattg acgatcatgc
     3841 gaaaaaaggg aaagcatatt gctatcttaa aagatgaata cggagggaca agcggcttag
     3901 tgacgattga agatatttta gaagaaatcg tcggggaaat ccgagatgag acagatttgg
     3961 acgaagcatt gatagcggaa cagtcagatg gctcttatat tatctccggc aaactaacat
     4021 tagatgattt ccagcgttat tttcatgtag agattccaga gtttgaagaa acgaatttta
     4081 ctacacttgc gggctttgct tctagccgat ataaagaaat aaaagcagga acaatcattg
     4141 agattgcatc attccgtttc acggtattag aataccagca tgcacatatc gattatttca
     4201 aagtagagtc cacggaaaga aaaacagaat aaaagaaaaa aggctgtgac attttttgtc
     4261 acagcctttt tcctacgatt agtcaagtaa aaggtgcaag aaaaatagct cgtaaacgaa
     4321 agcgacaaaa tcaaagcaca ccaaataaag ccccgttcgt aaaaaatttt cgatctgggc
     4381 aaagaagtgg tatactgtaa gtggtttagt taatggtcca ctgagaccgc gtacgcatag
     4441 tcgtactctg tatgtgccct aaccatagcg tgcatacatt tcagaagctt gttcatacaa
     4501 gcaactgtgg caaccttatc ttt
//
LOCUS       DACEMS010000147         4465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEMS010000147 DACEMS010000000
VERSION     DACEMS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.154"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            161..391
                     /locus_tag="IW735_002473"
     CDS             161..391
                     /locus_tag="IW735_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1045437.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IW735_002474"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IW735_002474"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1045438.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IW735_002475"
     CDS             1802..1978
                     /locus_tag="IW735_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1045439.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IW735_002476"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IW735_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1045440.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IW735_002477"
     CDS             2593..3060
                     /locus_tag="IW735_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1045441.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IW735_002478"
     CDS             3145..4029
                     /locus_tag="IW735_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1045442.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IW735_002479"
     CDS             4146..4379
                     /locus_tag="IW735_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1045443.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1524 a          678 c          970 g         1293 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa ccttt
//
LOCUS       DACEMS010000148         4429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEMS010000148 DACEMS010000000
VERSION     DACEMS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.155"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(279..809)
                     /locus_tag="IW735_002480"
     CDS             complement(279..809)
                     /locus_tag="IW735_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1045444.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            914..1465
                     /locus_tag="IW735_002481"
     CDS             914..1465
                     /locus_tag="IW735_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1045445.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1618..2091)
                     /locus_tag="IW735_002482"
     CDS             complement(1618..2091)
                     /locus_tag="IW735_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1045446.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2229..3701
                     /locus_tag="IW735_002483"
     CDS             2229..3701
                     /locus_tag="IW735_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1045447.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3698..4408
                     /locus_tag="IW735_002484"
     CDS             3698..4408
                     /locus_tag="IW735_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1045448.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1390 a          807 c          853 g         1379 t
ORIGIN      
        1 caaaacgctg atttcacaac ctcttgataa acggtgttca aacggctgct tctcggtaat
       61 aagcccacta gcctagaaaa tatgaaaagc tattttctaa ctagcgttct tattactcaa
      121 agcataacgc tgatttcaca acctcttgat aaacggtgtt caatcagctg accctcggta
      181 ttagctcaaa aaacggcaaa acatgaggaa ctatttttcc gtttctttct ctaatactca
      241 ggtcagaacg ctgatttcac aacctctctt tcacaacctt attttactcg tttataattt
      301 tcatcgaagt attttccgct acggatatca tctggcattc catcgtatgg ttcttcgcta
      361 ataacatctt cattgtctat acctgcatca cccatataag tgatcgtagc aaattcagga
      421 acaagtaatt ttggataagt ttcatatttt tcacgagctg cttccatttc tgcgatatgt
      481 tcgttttgga aagtaacagt gatttctgga tgttcttcaa cccatttagg gaaaaagatc
      541 attccgtgaa gtttcttttc atttggcata aagtccaatg ccacgtctgt ccctgttggc
      601 tctgtccaag tgattttgaa tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct
      661 tttacccaac ggcctttgac cattccgcca tggatacggt aatcaacagt atggtcattt
      721 ttggcatacc attcatattc ccaaccattg tcatacgtat agataaagtg agttccaata
      781 aaatcttcca attctttaaa ttctttcatg tttaagcatc tccttataaa ttaaaacatg
      841 taataaacta catgttttcg attactcatt tatgatagaa tggatttaaa gatttgtcaa
      901 ggagggaaaa ctgatggcaa gaaaaaatac acttcgatat attttgttag ggttattaag
      961 taagaagaag atgacaggat atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt
     1021 ttggcaagca aaacatagtc agatttatcc tgaacttgca aaaatggaag agcaaggaat
     1081 cattcagcat caagtagaga tcacaggtga aaaattggaa aagaaagttt atgaattaac
     1141 agaagaaggg aaggaacaat tggcagagtg gattcataca cccaccaatg aactaccggt
     1201 taatcgagac gaatttgttc tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt
     1261 agtagaaatt attcagcagc agcgtgacct tcatgaagaa aaacgacttc atctattaga
     1321 gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat gaaaattacg gacactacct
     1381 gattttagcg catgcgatca atcgggaaac agaatatgca aattggctaa cagatgtatt
     1441 agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca gtgatggaga attcctttct
     1501 atatgaaaga aacgcagatc accgttcgga tattcttatg ttcaaacagg ccttttttca
     1561 agtatttatg tagcatctac ttattaaaaa gcttggtact taggagatta gtggcgttta
     1621 gagttctttt aacaactgtt cgtagtcagc tttttcatcg acaggaagga gcttgtaagt
     1681 taattcgtct gtctcgttgt catgaatgat gtacatctta gcattctctt ctttgaagtt
     1741 accgatttgt aaaatcgtat agcgttcgtc gatcaattcg atatctttga ttgcttttga
     1801 aaaaacttta cggacatggt gcattttctt ttggcggatt tctgcctttt ctgcagcttt
     1861 tgacaaagta aatccgtcat ctaaatattt tttgatcagt tctactttta tgactgtagg
     1921 taaacgatat tttctaggac aattcgcatc agcttcgatc gaacgaatga atcccttttg
     1981 ttcccaatac cttaattgac gtggagacac acctacgata tcacttaatt cactgatgcc
     2041 aacaaccaat tgatcatttt ccaacaaacg tttaagggaa gccccaacca tttttgtcac
     2101 tccttttgtc ctcttgcttc ccttaattat atccgtatta acttatgtgt caagtaggtt
     2161 gacaaatagc gaatttagga ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa
     2221 ggagaaatat gagtaacaac caacccgtag atatttacgg aaaaccatat aatcggacac
     2281 ttttagttgt tgtcttatta attggtacat tttgtaccgt tttgaatcag accttattga
     2341 cgacagcttt tcctacattg atgaaagctt tcgatatttc agcgtctgat gtgcaatggt
     2401 taacgactgg tttcttgcta gtcaacggga tcatgatccc aataacagca tggctgatca
     2461 ataaattcag ttcaagaaat ttatatcttt ctgccatgac gatctttttg atcggaacga
     2521 ttacttgctt tacagcacct aattttagta cattactaat cggtcgtttg atccaagctt
     2581 gcggtgtggg agtatcgatg ccattattgc aaaatatcat gctatctatt ttcccgccag
     2641 aaaaaagagg atcagcaatg gggatgtcag ggatcgttat tggacttgct cctgctttag
     2701 gccctacatt atctggttat atcattgata actaccattg gcgtgacttg tttggtatgg
     2761 tactgccaat cgttgttctt gtcttggttt tagcattctt cctaatgaaa agtgttattc
     2821 cattgtcaaa tcctagcatt gatattttat ccgctatttt atcaacgatt ggattcggta
     2881 gtttacttta cggattttct agtgtaggag atgacggctg gggaagcttg aaagtaattg
     2941 gtttcttagt tgttggtgtg attttcatcg ccttgttctc atggcgtcaa ttacatttgg
     3001 aacatccatt cttggaactt cgtgtattca aatcatctac attcacgatt gcagctgttt
     3061 tagctggtgt aacaaatatg gcgatggtag gggcagaaat ggtcgtacca ttgtatattc
     3121 aaaatattcg tggagaatca gcattccatt ctggcttgat gttgttgccg ggtgcattga
     3181 tcatgggggc tatgatgcct gtgacgggtg cgattttcga taaatacggc gcgaaacgtt
     3241 tagccatcac agggatgttc attttgacag cggcaactgc accatttgct ttcttgacaa
     3301 aaacgactcc agttttatat atcgtgatcc tttatgcgat ccgtatgttt gggatctcca
     3361 tggtcatgat gccggtaacg acttctggta tgaatgcatt gccaaataac ttgatcagtc
     3421 atgggacagc ggtaaataat acattccgtc aaatcgcaag ctcgattggt acagctattt
     3481 tgatcagtgt gttgacgaat gtgacaaaag caaatatccc aagcaaatct gtattggata
     3541 atacgccatt agcttataaa gatcaggcaa tcaatgccac actgtcaggt taccatgccg
     3601 cgtttatcgt agcgatcatc tttgggattc ttggctttat tattgccttt ttattgaaga
     3661 aaaaagaacc tgttctaatg aaagcggggg ataatgcatg attattggag ttttgattgt
     3721 cagcttgctt gcttttgctt taacgaacgt gttcgctaaa aaaacatggc aaaccttttt
     3781 atctttgatt ttcggactaa tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc
     3841 caatcacttt ggaatggaaa aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc
     3901 tgattcccaa ggagcagata tgcttttgta taaagcttta gggaatggaa aagaaaaagt
     3961 gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa gcaactggta cggataacga
     4021 aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg
     4081 ggaatacaaa aacgacatgt ataaattctg gttcaatatt gctgataaca atcacgaata
     4141 tgacagccga gtgaatacat tcaaaatccc agagacatgg gtagaattaa gtacggacca
     4201 agcagccaag ttagctgaat tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa
     4261 agcttatgtt caagacggta tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc
     4321 agagcaagaa caacgtacga aagccttagc tgctgaattc caacaacaag cattcgcaaa
     4381 attagtgaaa gaagcaaaag gcgaataata ttttgaacac cgtttaagg
//
LOCUS       DACEMS010000149         4428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEMS010000149 DACEMS010000000
VERSION     DACEMS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.156"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW735_002485"
     CDS             complement(543..2048)
                     /locus_tag="IW735_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1045449.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW735_002486"
     CDS             complement(2045..2731)
                     /locus_tag="IW735_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1045450.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW735_002487"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW735_002487"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1045451.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1237 a          919 c          729 g         1543 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaac
//
LOCUS       DACEMS010000150         4395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEMS010000150 DACEMS010000000
VERSION     DACEMS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.157"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            712..2571
                     /gene="ltrA"
                     /locus_tag="IW735_002488"
     CDS             712..2571
                     /gene="ltrA"
                     /locus_tag="IW735_002488"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1045452.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
     gene            2692..2949
                     /locus_tag="IW735_002489"
                     /pseudo
     CDS             2692..2949
                     /locus_tag="IW735_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2952..3105
                     /locus_tag="IW735_002490"
                     /pseudo
     CDS             2952..3105
                     /locus_tag="IW735_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            3128..4333
                     /locus_tag="IW735_002491"
     CDS             3128..4333
                     /locus_tag="IW735_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1045453.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         1588 a          750 c          893 g         1164 t
ORIGIN      
        1 aaaaggttag gcacttaaaa gaagaaaaca taagtttcct tttaaggaga actgatattt
       61 tgagcagtgg aatgatgggg taacgccctg aaacgctccc ttaatactcc gactggctgt
      121 ttaacatata gctgttagat ggtcagaagc tcggtgaagt cggctgaaag ttaccgtaaa
      181 tatgataaat tcatatacga aagttgttca gaggtgaatg atgtaaccta taggtcggag
      241 gtctataaaa tactcacggt gagaatgtaa gtaatactac tgacgaactt gcgaatgaaa
      301 gaatctatac acggactgta tagaaatgta caggattcta ccgtatcaac ggaacagaat
      361 gtatgttgtg gtttagtaag attattggtt atgaacaacc atgcaatgtt acaggcattg
      421 gaaatataca ggttcaaagc aagcacctaa aagtatatat aaagataaaa atatcgaaac
      481 atgggaagct ggcaatgtgg aggaaactat attccattga aacatttgtg acgaagaata
      541 ataagcacat ttatgccagt gaaagtggtg gcacagtacc aatgaggata tagaaatata
      601 tcggaggaat agccactagt caaaagaaat cgttaattaa atgatgtatt tcatagaatc
      661 gagtaagaca aaataaacac tacaacctaa gtaaacaaag gtagtggtgc tatgaataaa
      721 gtaaaaacaa ataaaacact tcgtcatagt gaatactatg gcatgacaca aatatttgat
      781 gaactgtatg taaaaagtga taaaaaagaa gtttttacag agctcatgac aattattatg
      841 agtgatgaaa atattcgact agcatacaga aatatcaaga ataacaaggg cagttataca
      901 gcgagtaccg ataagaaaag tattcgagac attaaaacac tgtcagaaga tacctttcta
      961 actgaggtta aagttctttt tcgctgttac aaaccacgag cagttagacg tgtagaaatt
     1021 cccaaaccaa atggaaaaac acgccctctg ggcattccat caatctggga taggttaata
     1081 caacagtgta ttttacaagt cttagaaccg atatgcgaag ctaaatttca caattatagt
     1141 tatggatttc gaccaaaccg ttccacacat catgcgattg ctacctgcaa tcgactggca
     1201 aataggggga agctacactt tgtagtagat gttgatatta aaggattctt tgataatgtc
     1261 aatcactcta aacttttgcg tcaaatgtgg acgattggaa tccgtgataa atggctatta
     1321 ggtattattc gatccatgct aaatgctcct atagtcctac caaccggtaa agtcgaacac
     1381 cctaaaaaag gtgtacctca aggtggtgtc ctatcccctt tactatctaa tatcatattg
     1441 aatgaattag attggtgggt tgcttcacaa tggcaaaata ccacgattga gacagaaaag
     1501 attatggata ggcgagataa aggcaaaggc attgataaga ctaatcagta tcgggcatta
     1561 agacgaacaa acttaaaaga aatatacatt gttcggtatg ctgacgactt caaaatcttt
     1621 tgtagaaaac gaagtcatgc agataaaata tttcacgcaa cgaaaatgtg gcttaaagaa
     1681 cggttatcac ttgaaattag cgaagaaaaa tccaaaattg tcaatctcaa aaaaggtaaa
     1741 tcagaatatc taggatttga attaaagcta atcaagaaag gtaaaaaata cgtagtcgaa
     1801 agtcatatga gcaaaaaatc aatgacaaga gttaaaatcc aattaaagaa acaattacgt
     1861 aaagttgcca gaccgaagaa tcattgcgaa caagcaaaag aaattggatt atataattct
     1921 atggtgattg gaatccatca atattatgga attgcaacct gtgtaaatct cgattgcagt
     1981 aaaatagcct atacactaaa accgttcatc aatcacaaac tgccgacaaa gaaacatgga
     2041 aagatattaa atacatttat caaatcgaag tatggaaaaa gtaaagaaat gcgatggttg
     2101 aataatgtgc caattgtacc gctcaactat gttcagttta gacttgctgt ccccctatca
     2161 gagggaactt gcaagtacac tgaaagtgga cgaagcaaga tacacagtaa attaaaatta
     2221 gacctagccc ttttacttca catgatgaga aatagtaatt atgaaagaag tattgaattt
     2281 gtggataaca gaatatctaa atattctgct caaaagggaa aatgtgctat cacgggtaaa
     2341 tttctcgaat acgaagaaat ccattgccac catatcgtac cagtaaaaca acagggtaca
     2401 gacaaataca gcaacctcat tattattcac aaaaatgttc acgcattagt ccatgctatc
     2461 gaagaaaaaa tcatacacaa atacctgaat gtattaaatc taaccaatga acaaatagaa
     2521 aaactaaatg aattacgtgt caaagctggt aatagtgttt taacagttta acttgaaaaa
     2581 tatatcatac ttaacattct tttgatggaa cgccgtatga agtgaaagtt tcatgtacgg
     2641 tgtgaagcgg gggaaaatct ggagatgaca tcaaaggatt acctatcgct atttaatttc
     2701 agagtggctt taggtcgtat gtctgaaatg ggctatggca tgatgtttgg cagtgacgta
     2761 caaaaggatt tcttcttaaa gcgaatcaaa ggtcgtggct atgttgatgt aggaacaagt
     2821 gtcatatcag agttttatac tccccttgta ccaaaaggat atgatttctt ggaggaaatt
     2881 aaaaagttat ccaacagcag acagtccacg caggcgacgt gcgaagcgga agtcgcaggt
     2941 gtggactgat cttgctggct ggtgtggcaa tagccacgcc agcacttaac ccctcggtat
     3001 ctaacagggg ggtacaaatc gacaggaaac agtcaaaaaa acattagaaa atcctttggt
     3061 tacaagggat ttacaaaatt tcagcgtatg tcaaatgggc tttaaaagtt gacatacgcc
     3121 tttttgattg gagggatttt tactgaatga acaaacttgg ttacagcatt taaaagaaaa
     3181 acgcttggct tatggactat ctcaaaaccg tttagctgtt gcgactggta ttacaaggca
     3241 gtatctaagc gatattgaaa caggaaaagt caagccatca gaggatttac agcagtccct
     3301 ttgggaagct ctggaacgct tcaatcccga cgctcccctt gaaatgctgt ttgattatgt
     3361 aagaattcgc tttccgacaa cagacgtaca gcaggtggtc gaaaacatct tacaactgaa
     3421 actgtcctat tttcttcatg aggactatgg tttctattct tattcagagc attatgcttt
     3481 aggcgacata ttcgtccttt gctcccatga actggacaaa ggagttctgg tggaattgaa
     3541 aggtcgtggg tgcagacaat ttgaaagcta tcttctggca caacaaagaa gctggtatga
     3601 gttctttatg gacgttttgg tggctggcgg tgtgatgaaa cgccttgacc ttgccattaa
     3661 cgataagaca gggattttaa atatccctgt actcactgaa aagtgccaac aggaagaatg
     3721 tatctccgtc ttccgcagtt ttaaaagcta tcgcagtggc gaactggtac gcaaagagga
     3781 aaaggaatgt atgggaaaca ccctctatat cggttcatta caaagtgaag tttatttctg
     3841 tatctatgaa aaggactacg agcagtacaa gaaaaatgat attcccattg aagacgcaga
     3901 agtaaaaaac cgttttgaga ttcgattgaa aaatgagcgt gcctattatg cagtccgtga
     3961 tttactcgtc tatgacaatc cagagcatac cgcctttaaa attatcaatc ggtatatccg
     4021 ttttgtagat aaagacgatt ccaaacctcg ttctgattgg aaactgaatg aagaatgggc
     4081 ttggtttatt gggaacaatc gtgaacgatt aaaactaacc acaaaaccag agccttactc
     4141 cttccaaagg acgctgaact ggctatctca tcaagttgcc ccgaccttaa aggttgcgat
     4201 taaacttgat gaaatcaacc agacgcaggt tgtaaaagac attctcgacc atgcgaaact
     4261 gacagaccga cacaagcaga ttttgaagca acagtcagta aaagaacagg acgtgataac
     4321 aacaaaaaaa taactcaaat acaaattcat tgaatataga gaggagaaca tttttatgaa
     4381 ttttggacaa aacct
//
LOCUS       DACEMS010000151         4278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEMS010000151 DACEMS010000000
VERSION     DACEMS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.158"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            149..1072
                     /locus_tag="IW735_002492"
     CDS             149..1072
                     /locus_tag="IW735_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1045454.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1090..2331
                     /locus_tag="IW735_002493"
     CDS             1090..2331
                     /locus_tag="IW735_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1045455.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            2465..3811
                     /gene="gor"
                     /locus_tag="IW735_002494"
     CDS             2465..3811
                     /gene="gor"
                     /locus_tag="IW735_002494"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1045456.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1473 a          674 c          928 g         1203 t
ORIGIN      
        1 caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt ttgtgaaata
       61 attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga gctatgctac
      121 tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag tagaaactat
      181 tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg gtgaagagca
      241 atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat tggtagaatt
      301 agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc atcctgaaaa
      361 aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag atgtgatcac
      421 agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc gtataccgta
      481 tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct gggcgaaaaa
      541 aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga aggcgatgga
      601 agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca tccatcaatc
      661 caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac aaaaagatcg
      721 tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt ctatttatga
      781 agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga atgaattgaa
      841 tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta ctccaggcga
      901 aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt tagaaggtag
      961 ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc atcctgaaaa
     1021 ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat aaggagtatg
     1081 atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc gccagcagga
     1141 acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata tattggcggg
     1201 aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat ggctgaagga
     1261 gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat ggtcacccat
     1321 gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt cggtatttct
     1381 gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc cccaggtctg
     1441 ccgatccatt tatccactca agcttcagca actaattttg agacattgga attctggaaa
     1501 gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga agtggcagag
     1561 attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat gtgtatctct
     1621 tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa ccgtggcgga
     1681 tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg tcaagaacga
     1741 aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc agtggatatg
     1801 tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt caagatcgaa
     1861 ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa agcggctgta
     1921 gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat cgacgagtta
     1981 tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc aactgaaaac
     2041 gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg tgaagtatta
     2101 tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt ttctgtagga
     2161 gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt tgaagttatg
     2221 cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat tttaactatg
     2281 ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata aggaaaaagc
     2341 tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg ttacacttgt
     2401 tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa taaggaggaa
     2461 caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta ttgcttcagc
     2521 aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg aattgggcgg
     2581 aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa gtacgatttt
     2641 ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa aaaatgttga
     2701 tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg gtgcttatca
     2761 aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt ttatcgatag
     2821 tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga tcgctactgg
     2881 aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt caaatggatt
     2941 ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat acattgcggc
     3001 tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat atcgaaaaga
     3061 acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa tgtaccaaga
     3121 agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa acaatgacga
     3181 atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac tttttgccgg
     3241 tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg aattggacaa
     3301 aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata tttttgctgt
     3361 tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg gtagacgttt
     3421 atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac ttgttccgac
     3481 agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag aagcattgga
     3541 aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa tgtactttgc
     3601 attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa aagaagaaag
     3661 aatcgtcggc ttacatgcta ttggtgttgg tgtcgatgag atgctgcaag gttttgccgt
     3721 ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca tccatccaac
     3781 aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca acagcttttt
     3841 attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt ttttgttttt
     3901 tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag ggaagttgga
     3961 gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta tgcactctta
     4021 agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag aacagatgtt
     4081 taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg ttaaaagaat
     4141 accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga atcaaatcaa
     4201 gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc cgaacaaata
     4261 gtgttcaatc agctgtcc
//
LOCUS       DACEMS010000152         4218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEMS010000152 DACEMS010000000
VERSION     DACEMS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.159"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            10..210
                     /locus_tag="IW735_002495"
     CDS             10..210
                     /locus_tag="IW735_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1045457.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="IW735_002496"
     CDS             288..962
                     /locus_tag="IW735_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1045458.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            1195..1629
                     /gene="dcm"
                     /locus_tag="IW735_002497"
     CDS             1195..1629
                     /gene="dcm"
                     /locus_tag="IW735_002497"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1045459.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            1630..2337
                     /locus_tag="IW735_002498"
     CDS             1630..2337
                     /locus_tag="IW735_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1045460.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            2327..2617
                     /locus_tag="IW735_002499"
     CDS             2327..2617
                     /locus_tag="IW735_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045461.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2876..4060
                     /locus_tag="IW735_002500"
     CDS             2876..4060
                     /locus_tag="IW735_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1045462.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4075..4194
                     /locus_tag="IW735_002501"
     CDS             4075..4194
                     /locus_tag="IW735_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1045463.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         1364 a          692 c          979 g         1183 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
     1201 tttttagatt tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat
     1261 cattgtatcg gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat
     1321 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa
     1381 tccctcggac cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg
     1441 ggaaagcggc aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
     1501 accactcttc tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac
     1561 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg
     1621 gagtggtagg tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt
     1681 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca
     1741 acatctgatc aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt
     1801 ggacaagtct atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa
     1861 atcgcactgc cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa
     1921 caaggtaatc gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt
     1981 ggtacaaaaa tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac
     2041 agtgtgaatc tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt
     2101 gttcatacct tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg
     2161 aagctaacac ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga
     2221 gattctcaag tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt
     2281 ccggtcattt ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga
     2341 gttgattgga gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga
     2401 acaattgcga cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc
     2461 atatttaatg aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt
     2521 tgagcggatt gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa
     2581 agcagagttg gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt
     2641 taggcgctga ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca
     2701 tggacggcgt tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac
     2761 acaaaaaata agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt
     2821 gtataggtgt taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc
     2881 acaacggaat ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca
     2941 aaataaattg gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg
     3001 tggcgtaacc gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa
     3061 tcccgatttg cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
     3121 tgcacgaaaa attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga
     3181 ttatgccttt tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc
     3241 tcatgaagaa gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga
     3301 aactttttta ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc
     3361 cggtggcgtg atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat
     3421 acctgagcta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa
     3481 aagttatcgt tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt
     3541 atatatcggc agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca
     3601 atatatcaag ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg
     3661 actgaaaaat gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga
     3721 gagcaccacg ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa
     3781 aaggcgaacc aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa
     3841 agagattcaa ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct
     3901 aggccgacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac
     3961 aacctatatc caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt
     4021 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga
     4081 attgtaaaag atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc
     4141 ttgatgaatg caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat
     4201 gaatttcgga caaaattt
//
LOCUS       DACEMS010000153         4215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEMS010000153 DACEMS010000000
VERSION     DACEMS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.160"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            624..3263
                     /locus_tag="IW735_002502"
     CDS             624..3263
                     /locus_tag="IW735_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1045464.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     VSQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3431..4129)
                     /locus_tag="IW735_002503"
     CDS             complement(3431..4129)
                     /locus_tag="IW735_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1045465.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          804 c          911 g         1222 t
ORIGIN      
        1 ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatca tttgacaaag
       61 agccagaaaa agccgagctc tcgataagct cggctttctt gtacctcgga aggattcaaa
      121 taaacagaaa tgattgtaac tttccatcat acatgcaaag cacggtgttg aatttcttca
      181 tgttaaacaa aaattttgtt ttcgttacat cttcaacatt tgaaacatct cttgttccta
      241 ttcaattttc agacgtttac gttattcaat tgttacgtat cgcattactt cttgtttcgg
      301 atacttcgtg atccaaacat ccatacgcat tcactattta taggtttgta ctctacacgc
      361 atgtctgggt gaaacctgta cttctaacaa ctttatttgc ttacactgtt ctatcgagaa
      421 attcttctgt ctatttgatg gtatcctcct tccttacatc tatataatag catcctttgg
      481 tagcgtttgc aaatataagc actttaaatt ttacgctttc ttaacaaata atcgataagc
      541 cctttatttg aagtaaataa aaaaaggatg ttatagtaaa ataagtacct ttttttgtac
      601 gaagatttta aaggaggaat aggatggaag aatacaagca gacaatagaa gaagtaaaga
      661 aggtaacaga agcagatcca gatactgggt tgtcttcaca agaagtggat gaacggcgaa
      721 aaaagcaggg attgaataaa ttcgatgagg cgccgaaaga atcgatgatc aaaaaattct
      781 ttcgaagctt atccgatttt acaacgatca tccttcttgt agcagcggtg atttcctttt
      841 ataccgcatt tgctacagaa catggggatt tatttgaagg actgctgatc atcgctatcg
      901 ttgtgatcaa ctcggtattg gcgattgtcc aagaaggaaa cgcagaaaaa gcactggaat
      961 ccttgcaaga tatgaataaa caaacggcaa ctgtcatacg agacggcaaa gtgaccactg
     1021 ttgagtcaga gcaactggta gtgggtgata ttcttgtttt agagtccggt gatgctattt
     1081 ctgccgatgc ccgtttgatc gaagtatcac aattacgtgt agaagaatcg gcgttgactg
     1141 gagaaagtga agcagttgaa aaagatgcag catttattgc tgaagaagat gagtcattag
     1201 gtgaccaact taatatggta tttaaaggat gtacggttgc tgcaggtcgt gggaaagcga
     1261 ttgtcactgc cattggtatg gctacagaaa tgggtaagat cgcggatctt ttgaacgaaa
     1321 atactatgca aaaaacacct ttacaggtcc gattgaatca gctaggcaaa cgaatcagta
     1381 tgatcgcttt agcggctgcg gctttagtat ttgtgattgg tgaactgcaa ggagaaccac
     1441 tccttgagat gttcatgact tcgatttcct tggctgttgc agctgtaccg gaaaccttga
     1501 ctgttattgt tacgttgaca cttgcatacg gtgtgcagaa gatggcgagg aaacatgcga
     1561 tcatccgaca gttgccagca gttgaaacgc taggaaccgc taatgttatc tgctcagaca
     1621 aaacaggaac gctgacacaa aatgaaatgc gcgtccgccg ggtatggagc aaagaagatg
     1681 aagtaacgaa tatcgaagat tcaatgacga acagcgcgat ggaaatattg aaaatggctg
     1741 cactatgtac agatgtgacg gtcgagcaag aagatgacga ccttgttatc aaaggtaatc
     1801 caacagaagt agccatcgta agggctgttg aagagaatta tcatacaaaa gcagaattag
     1861 aagaaaaata tccacgagta aacgagcttc cttttgattc tgaacgaaaa atgatgacaa
     1921 cagttcacca aatgggcgaa aaatacatct caatcaccaa aggagcattt gatgttcttg
     1981 ctccgcgctt tagttcaggc gatgtagaac aagcggcgat cgttaatgat cgctttggta
     2041 aacgggcact gagagtgatt gctgttggtt atgctactta tgacgaagaa ccgcaagata
     2101 tttcttctga agcactggaa aaagacttac gtttgattgg tctgatcgga atgatcgatc
     2161 caccacgtcc agaaagtaaa ggagcaatca agcgagcgaa aaaggctggc atcaaaacag
     2221 tcatgatcac tggagatcat gtggtaactg ccagtgccat agcgaaagaa ttaggtatct
     2281 tgaatgatcc gtcagaagca ctctcaggat cagaactgca tcaattatca gacgaagaat
     2341 tagatgcgcg tgtcaaagct ctctctgtct atgctcgggt aacgccagaa gacaagatcc
     2401 ggatcgtcaa atcttggcaa cgtacgggtg cagtcgtagc gatgactggg gatggtgtga
     2461 atgatgcacc agcgttgaaa gcaagtaacg ttgggtgtgc catggggatc acagggacag
     2521 atgtggcgca aagtgccgca gatatgattt tgacggatga caactttgcg acgatcgtag
     2581 atgctgttag tcaagggcgt tcagtttatc agaatattcg taaagcaatc aactttttac
     2641 taagctgtaa tatttcggag atctttattg ttttgattgc gatgctgtta ggatggggag
     2701 caccatttac agctgtgcaa ctactgtttg tcaatgtagt tgctgatgga cttccaggat
     2761 ttgctttagg aagagagcca gcggaacacg gcatcatgga tcagccaccc attccgaaaa
     2821 atgaagggat ctttgctcga ggtttattgc aaaaaattgc cataaatgca ggggtcttca
     2881 ccatcgtaac cttgtttggt tattatttag gtagttatgt cgatactatc agcccatggg
     2941 tagacgcaag tcagcatgtc ggtcaaactg ttgcattctt gatcttagct tattcatcga
     3001 ttctgcatgt gttcaatgta cgcagcagcc aatcgatctt caaagtcaat ttagcaacca
     3061 ataaagcttt ggtagaaatg gcgttactgg cattagctat tacaacggct gttgctttgc
     3121 tgccatttac gcaagaacta ttcggcctag tacacatcag tttgaatcac tggttcttgg
     3181 tagggatttt atctatcgta ccaatcgcgg tgaatgaact gatcaagttc catcgattac
     3241 cggaagcaga agaagaggaa tgattaactg tttctaagtt gattttcttt ttactggaac
     3301 tatacaaaaa ggaggtgtga catgaatgtt gtcacacctc ctttttgcta atgggctgaa
     3361 tgtttctttt actgcctctc gataaacggt gttctcctac caacccctcg gtcacaagtc
     3421 acactctctt ttaaatatct tttggcaatt gttcataaaa tgctgctttt gttgcggaaa
     3481 tgtatgggct gagccataga taaccaatac cagcagttgc taatgctaag atatgccagc
     3541 cgataaatga aagatctaac cagaaaagtt gtcctttata accatccatc aattttcgac
     3601 ttgcagtgat cgtatccagt actttaggtg tttgtcctgt ttcttggacg atatcgtagt
     3661 agataaaata gctttgagaa taagaatagc ttttaatgat tccaggaata actaatagca
     3721 aaccccataa tgttgtaaag atcgagatca gcacagctaa gccgatcact ccgccgataa
     3781 acaaaccttt gaatccacga aatgcatctt tgaatggttc gatctgatct ttttgtccac
     3841 gtaaaatatc aagataagtc caagaaatac cgctcatgaa caaagcgcta atgatgctgg
     3901 aaataattcc gccaccgcct cctgaactac cgcctcctga agttgtcgca tctgagacgc
     3961 tcgctgatcc atcatatatg gtggtggcaa ataagataat aggaatcata ataagaagaa
     4021 tagcagcaat accaatcaat gtagggatta gatttagtaa aacagcttgt ccccaacgtc
     4081 cttttagact ctcttttgct tggcgtttta attctgcaga agattgcatg atttttccct
     4141 ccttttcttt aataacacca atataccatg tatcaaaaaa tatttctgtc ataatgtttt
     4201 tctctttttt gttat
//
LOCUS       DACEMS010000154         4200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEMS010000154 DACEMS010000000
VERSION     DACEMS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.161"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..261)
                     /locus_tag="IW735_002504"
     CDS             complement(<1..261)
                     /locus_tag="IW735_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1045466.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMS"
     gene            complement(416..742)
                     /locus_tag="IW735_002505"
     CDS             complement(416..742)
                     /locus_tag="IW735_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045467.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(750..953)
                     /locus_tag="IW735_002506"
     CDS             complement(750..953)
                     /locus_tag="IW735_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1045468.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            1028..1678
                     /locus_tag="IW735_002507"
     CDS             1028..1678
                     /locus_tag="IW735_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1045469.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1733..2899
                     /locus_tag="IW735_002508"
     CDS             1733..2899
                     /locus_tag="IW735_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1045470.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(2988..3380)
                     /gene="rpsI"
                     /locus_tag="IW735_002509"
     CDS             complement(2988..3380)
                     /gene="rpsI"
                     /locus_tag="IW735_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1045471.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3396..3839)
                     /gene="rplM"
                     /locus_tag="IW735_002510"
     CDS             complement(3396..3839)
                     /gene="rplM"
                     /locus_tag="IW735_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1045472.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         1378 a          753 c          656 g         1413 t
ORIGIN      
        1 tgacattccc gaaatttcta cgagattttt gatttcaaat atcaaaaaaa ttttttgatt
       61 ctcatcatac aagttatttt tcaatgaaag tctatttcta tcttttagta acatataaac
      121 taacttagaa cttagtttca ttttcttata tttttcattt acgtttaaaa aaataggata
      181 ttgatagtat aattctgcat atatatcaga attagaatag ccttgctttt caactaagct
      241 ttcaacttct ttttcattca ttgtcacacc tgcttctgat tttatttgct ataatagatt
      301 caaataaagt tttctcagaa ctttattatt gagtgttccc ccgatctgca aactgacggg
      361 aacactcttt ttaatttttt ctacttcatc attattcaaa aaaacacctc gttttttaaa
      421 tttgatgact caatatccat tttttgcatt cttcttttga aaacaactcc attttttcta
      481 ataaaataac tggcaatccc attcttttaa atttcaaaaa agttgtatat gatacattca
      541 aatatttagc catctctttt ccttttagaa atggtctttc taacccggta tcaattcttg
      601 cttgatcaat agcctctttt gtcatttcaa aaacataatt tttcaattga ttcgtttgtt
      661 gtttatctaa cattactgta attcctgcat ccattatttt tctcctctca ttttctattt
      721 cttcaatcag ccaactcgcc aagttgtatt tatttagatt tcaaatcatt aagtaaccaa
      781 tttaccaact tttgataagt agatgctcta atactttctt tgctcccatt ttcgattttg
      841 acatatgttt tccgatttat acctatcaat tttgctgcat cgtttttagt aagtataaga
      901 tcggctctat gtcttcttaa gcttctaatg aattgatcat taagagttat cattcagcgc
      961 ctccttttaa attattcact ttgtggtttt tatcttaata agcacatttt aaaccacgaa
     1021 acgagtaatg tcaattaaaa cattactcaa tttgtggttt ttatgctata atagtttgga
     1081 atttactata gggggtgtta ctttgaatag aataaaattt tatattgatt ccagtaaaaa
     1141 gaccattaaa caaattagta cagaaactgg tattaactat acaactctta cgaactatta
     1201 ccgtggtgag cgaaatccta gaaatactga aacatggaat atccttgctg attatttcca
     1261 tgttcctgta agttatttga tgggattaga cgaaacaaat tccatagaac aacctaatct
     1321 tgatagaata aaaactgctc gtttagaaaa aaaggacaaa atagtcaaga atattgccag
     1381 tcttgactca gattatttta ctgaattatc taatctatac ttttccatga cttctttact
     1441 tgaagtcaaa cttcaagaag atgactccga ctttcttttt ttaaaaagta ttctagagga
     1501 ctcctttgaa tacacgattc tatttgccaa acaaaagaag tttattaata aagctttaaa
     1561 cttaaatatc gatgaaagaa aagagttaga atctgagtta tttggcaact taacccaagt
     1621 acaggttaac aatctaaaaa gagagataac agctcgatta atatcaaaac ttcaataaaa
     1681 ctaattattc aatcagccaa ctcgccaagt tatcagattg gagagattag ttatgtcaat
     1741 acaaaagtac acaacaaaaa gcggtgcacg atatagtgtc cgcttgtatg ttcgtaaaga
     1801 tgaaaatgga aagcaagaat actatgtaaa aaaagggttt aaaactgaaa aagaagccaa
     1861 gttacacgaa gcaagaaaaa aggtcgagat tgaagatacc ggctatcata agccttctaa
     1921 tgacttgttt ataacagtgt atgaagaatg gttgccttct tataggaatc gtgtacaaga
     1981 aacgacatat caacgtacaa aagacctttt ccgcctgcat atactaccaa aattcgggaa
     2041 taagtccatt tctaagatta ctcctgttca ctgtcagaat gcagtcaatg aatgggcttt
     2101 aaaattcaag aatatcaaac agcttaagtc ttacagctct caaatatttg agtatgccat
     2161 ttttgcagag ctattacacc gaaatcctat ggcaaataca aatctaccaa agcgggaaaa
     2221 gaaagaaaat gaaaattact tttcattaga agaactaaaa gctttttggg atattctcaa
     2281 taaagaagag cctttaaaac atattttaat cttccagctt ttgattacta ctggtatacg
     2341 taaaggagag ctagctgctc ttcattggtc tgacatttat tttgaagaac agctaatgta
     2401 tgttagaaag agctatgcaa ctattcgaaa tgaagagaag aatgcaagaa gaaaaactgt
     2461 caggattcaa aaaaatacaa aaaatatatc ttccgaacga atattaccaa ttgatgcaca
     2521 aactattgaa atgttaaaaa tctggaaaaa agaacaagct cgagagcttt tgcagtttgg
     2581 cataaataca aatacaaaaa atcaacttat ctttacctat gtaaatgcag acggtgacat
     2641 taatcaacct ttgcatgcgg actattcaaa taatatattg aaacgtttag agaagaaata
     2701 taaatttaag cacgttacta ttcatggatt ccggcacact cacgctactc ttctactaga
     2761 aggtggagca agtatcaaag aaacacaaga tcgattgggt cataaaaatg ctgaaactac
     2821 ccttaacact tattctcatg ttactgaaaa agctcaacga aatgcgttag aaaaattcat
     2881 tgcttacact ggtttataat tttgtcatta cctttctcat taccagaacg aaaaaaagac
     2941 accttctcgc aatgagaaag tgtctgaaat cgttggtata acaacgatta acgttttgaa
     3001 aactgtgaag ctttacgtgc tttcttaaga cctggttttt tacgttcgac catacgtgcg
     3061 tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc aacttctagc
     3121 aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc tccgccgttt
     3181 acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt
     3241 acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt tacagtaatt
     3301 ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg
     3361 atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt gatgtctaaa
     3421 acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt caattttgta
     3481 aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt ttcgatcaaa
     3541 cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc tcctgggtag
     3601 ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt cactttgtct
     3661 gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt tggtttattt
     3721 ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac atcagttgcg
     3781 tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt tgtacgcacg
     3841 ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc cggggctctt
     3901 tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg tcaacgtttt
     3961 tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt atataaaagg
     4021 gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc
     4081 cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccat
     4141 aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga acggcaggct
//
LOCUS       DACEMS010000155         4199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEMS010000155 DACEMS010000000
VERSION     DACEMS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.162"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(2..1222)
                     /locus_tag="IW735_002511"
     CDS             complement(2..1222)
                     /locus_tag="IW735_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1045473.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1242..1847)
                     /locus_tag="IW735_002512"
     CDS             complement(1242..1847)
                     /locus_tag="IW735_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1045474.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1896..2828)
                     /locus_tag="IW735_002513"
     CDS             complement(1896..2828)
                     /locus_tag="IW735_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1045475.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3253..4110)
                     /locus_tag="IW735_002514"
     CDS             complement(3253..4110)
                     /locus_tag="IW735_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1045476.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1191 a          849 c          692 g         1467 t
ORIGIN      
        1 tttattttac agaagcactc gaaactgcat ctactgcagt ttcatcgtgt gtgctggcgc
       61 tggttactgc atctgttttt ccaccatgaa cagatgcact tgaaaccgca tcttcatttc
      121 caacaagttg ttgacccgtt tgttgtaaat accactcaat gattggtttg aaatcaagaa
      181 tgtattcatt gattccagca tactctccat tttcatcatg cattgcttgg taattatgca
      241 caatatattt ttctggcact tgttttggta catgaacacg taccgcatct gttgctccgc
      301 ttcgcagttg ttgaattacc cactctacat ttttatacga tttttctggg tgacagttgg
      361 ctaatggatt ccctacctga ccgggcacac gagcagctaa catctctttc gcttcttggc
      421 gcaaattata atataaaaat tggttattat tatctgcaaa agttaattcc atcggcatcg
      481 aatctaaaaa gtaattgatt tgatccactg tcaataaccc acgatttaat ttgacgtaag
      541 tatttccaga aacggcaccg actgcttcag aagctttttc caaccaatga tcatctgaca
      601 tatcaacatc gggtattgtc gtagcaattg ttcctgtcgc atgcaaatct tctggtggaa
      661 gctttttagg ccctgctaag gtagacacga ctttaatgaa ttttacaaaa cttgctaatg
      721 tactgcctaa aaagtcaaca gttcgtggat caatcaagcg atcttcttgg tcaaatgctt
      781 cttttgcttt tcccaataaa aattcatttc cgggcatcac taatgcattc acgcctggtg
      841 cgtccaaaat ttggcgcaag tgtaattgcg cgcgtgaagt tccttgatcg tagtaagaag
      901 cacccacaac cattacaggt ttattttcaa gtggatggat tttaaatgat agccattcaa
      961 gtgcactttt caatcctgct ggaaccgtat gattgtgttc tggcgtagca ataattaccc
     1021 catcagccat taagattttg cgatttagct gttgaatagc aggactattt gtttgatcat
     1081 catcttgatt gaacagaggt aaatctttga tttcaatgat ttctaaatta aatagttcat
     1141 gatattgttt ttggataaat gttaataatt tacggttata tgaaagttca gctgctgatc
     1201 cgacgatacc tattaatttc atctttgttt gctcccctcg attaaatcgt ttcccatgaa
     1261 aagctttcgg cttcacgttt atttgcttgg tgcgcatgtt gcaattgact agtgattgtg
     1321 acaaagagta agaaatcttt gaatagttca cgtaattttg cgacttgttc ttcatcaatg
     1381 agtcgtcctt gctcatcaaa ggcttggagt gaatgaccca ataaaaattc tgagcttggc
     1441 ataattcgtg cctttaactc tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt
     1501 gaagaaccta acgtgccata agacgctccg gtaatcatca ctggtttatc cacaaacgga
     1561 aaaataccat atgaaagcca atttaatgcg ttaatcaatg aagcaggaac cgcatggtca
     1621 tactctggtg tgctgatgat tacgccatct gcttgttcaa tttttccagc aatttcttta
     1681 acaacatttg gtagttctgt gttttcaggt ttgttaaata gtggcagctc cttgatttcg
     1741 acaatttcga tttctgcctc atttacaaaa taatttttca taaactgtaa taattcacgg
     1801 tttgttgacc gcgttgaatt tgttcctact aatccaataa atttcatctt cattctcttt
     1861 cctttctaac atcgatttct gactgttcgc acgtactaaa ttttttcaaa tccttttgta
     1921 tacaagaatt gattctcttt tgttacaact gctccctcga tacccggctg ttcttcaagc
     1981 gctttcaaca cttctaaaga agatgctcca aataaacgtg tagtccagat ttctccatct
     2041 acagaagaat cagacaaaat cgtcaggctt gctacgtcat ttttaactgg aaagccagtt
     2101 ttcggatcaa tgatgtgatg atacactgac gaaccatttt gtagataacg ttcgtagatt
     2161 ccagaggtaa ctaaagattg gttgcttagt ttaaccaccg caagcgactc ttcacgtgaa
     2221 cctttaggat ttcgcaaacc aatacgccaa gcaccatccg aacgtcttgg tgattcacca
     2281 aaaacaacga tatttcctcc taagttaact aaagcaaacg ctgcatttcg ctctcggcaa
     2341 tgtgtaatta atttatctgc aatgtaacct tttgctaacg cccccaagtc aatcatcatt
     2401 cctggtttgg ttaaaaaaac ggttttcatt ttttcatcta attgaatttg gtgtggatcc
     2461 gttagcagaa gtaactcagt tatctctgct tttgttggaa ccaatgcatc ttcaaatcca
     2521 atgtgccatt tttgtacaac cggaccaata gcaatattca gataactatt tcttgcacaa
     2581 ctatgtgctt ttcctaattt gatgagctca aaaagttcag gatgtacaat cacagcttgt
     2641 cgtccagcag cttgattgat ggccattaat tcagaagttg catcatgtgc gctgaagcga
     2701 tgttcataaa cgtgcaacca ttcgcctaac tctgctagta atcgactagg ctcttcatgc
     2761 ccaacttgta atgtaattgt tgtccccatc atttgccaga ttttcgtttc ttcgatcacc
     2821 tgattcattt tgtaccttct ttacttgaac tacggattac atgagtaatt agtttttatt
     2881 tttgttactg ttttcacaac cagatgatac gcctgctaga tttatttgtc aaccgatttt
     2941 atatattttt ttatataata aggtaatttt aatataaaat tttatattgt aaaaaaacaa
     3001 ataagggaaa caaacaacac atttttgtgt gttgcttatt tccctctaac tatcgttttc
     3061 tatttgatgg ttaacctact taaaataaat actgcaacga caaacggaga tcaagcatgc
     3121 tttgtaacgt attttacgag aattacgact ttattttatt actctatctt gctgccatac
     3181 ccgtctatcc ctaatcatct ttggtgaatt ttttcaaagt tagcataaca aaaataagta
     3241 ttttttcctt cgttaatcaa tagtatcatt ttttttcata tgatttaatt catcaattaa
     3301 ttttttgttt ttattaattt tagcaaaagc tagtgcatct tgaatcaatt cgagcacttc
     3361 tgccttcgac ttatttttag cctgtgcgtt cttcgctaat tgggcagcag ctcgtgctaa
     3421 gtaataagtc aaatggttat ccgaacaaat atcgattgcg taatttagta gtgcatcact
     3481 tgtttccaaa tcaccgtctt gcgcaaaaaa aacaccacaa tgaaagacga tatttaacac
     3541 ccgccagata tcttctgttg tacgaatcgg atacgtataa attttttcaa acactcgatt
     3601 gaaataaact tctgcttttt ccaactcttt ttctctagca tagaccattc caatacctgt
     3661 atatgccaat aaacgaaaca acgtgttggc tgtttctgcc atcaacaact gatcaaaatg
     3721 gaacaacaca tccgtaattg gtgcattcgt aaaaatcatt tcaaatcctt ttagatagta
     3781 gtaacgtgca atcagctcag actcgtcaat taccttggga tcaatggctg ctaaaatctg
     3841 tgtcaattgt tcatattcac tcgtaatcag tgaaaactct gctttttcta gctgttcatc
     3901 aagttgtgta ttgatcacgt tcatcgaaga aaaaagttca actaacggaa gattgattcg
     3961 attacataat ttgattaaaa tttttaacga agggacttga ccattgttct caaatttact
     4021 taatgttgct tgtgtacaaa ttccttcaga cacttctttt tgagataagt tggcagcctt
     4081 tcttgccgta ataaattttt caatgttcat tattttctct ccaatcagac ctcacacgaa
     4141 aatcatttct gtctcgattt tagcatgttt tctttgggaa tgctttaatt ggttaagat
//
LOCUS       DACEMS010000156         4172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEMS010000156 DACEMS010000000
VERSION     DACEMS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.163"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            612..1424
                     /locus_tag="IW735_002515"
     CDS             612..1424
                     /locus_tag="IW735_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045477.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            3059..3613
                     /locus_tag="IW735_002516"
     CDS             3059..3613
                     /locus_tag="IW735_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1045478.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            3867..>4172
                     /locus_tag="IW735_002517"
     CDS             3867..>4172
                     /locus_tag="IW735_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045479.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT         1542 a          516 c          660 g         1454 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa caaatataaa
     1861 atttaataaa aatatagaac taaataggaa aacgtagacg taattttcta cgttttcctt
     1921 ttcatatata ttaaatatta cggaaattta tataaactaa tttagtgttt tgtttgtaat
     1981 tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatc agacattcta
     2041 tgctcatttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt atttgaaaat
     2101 tcttctttta gagtactttt attacgatac tagtatgata tatatctata gcaaacagtt
     2161 aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt tagatgaagc
     2221 gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct ttcaaattta
     2281 gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt atgtgacaaa
     2341 aagaactagt agaagaagga ataatggctt gataataaag tactacctat taaaggtagt
     2401 actttattta atttagtata aattaacata ctactatatt ttaagtttca tattatataa
     2461 attaataata tactaccatt ttttggtagt ataatttaaa ggtaataagt tattctttta
     2521 ttactatgtt tgggttttat agcaaggaaa gtttaataag agtttggacc tagcaaacaa
     2581 tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat ctacacagac
     2641 aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat taatgaaaag
     2701 cttggtagca cggttattca tatgctaatt tatttcactt tatagtaagg ggagttctta
     2761 gtatattgtc caaatttaaa tttcatataa tttaattatg taaattagct attctaagaa
     2821 cttctacgga taactgcccg tctaaaattt aattaagagc ttagaatctt catataagta
     2881 aggttaattt ttttgtacaa aatagtcgga atattctgag ttactgaaat gatataaaat
     2941 cataaactaa accccttgat tttcttcact tttaacagag tatatatata agttcatgat
     3001 tatggtaaaa tatatatata atcatgaact cagaatattt ttaagaaggg aaacaatagt
     3061 gagctataat gtacaaccgt taaggacaaa tgaagagata caagattttc agttttggct
     3121 aagaagaaca agtaatccag aaagagatag ttttttattt ttgtttggaa tcaataatgg
     3181 gttacgtatg tcagatatta taggattaaa agttggagac atacgaggta aaagtaagcc
     3241 tattattgtt gaacataaaa caggtaagcg aaaaccgatt tttatagata atttgagaga
     3301 agagatttta ttatatactg aaggtaaaga agaaaatgat tggttatttc caagtcggca
     3361 acaaggtcga catataacaa gggatcgtgt ctatcagata tatgcagata tagcagaaaa
     3421 attaggaaga gatgatatag gaacacatac attaagaaaa acttttgggt atcactacta
     3481 caagaaaact agagacattg ctaccttaat gtttattttt aatcatagct cgcaagcaat
     3541 tacaaaaagg tacattggta taactgaaga tgaaataggg gcatcgttac gtggttttaa
     3601 attgggaggc taattgagat attaggatat attttttgtg tataatgaaa acttagaagg
     3661 tgagattaat gaagataact gaattttcgg aagtatttaa aacgtttagt aacatttaca
     3721 atagaatacc agagatagaa caaaatgttg tagttgataa tgattattat atagagaatg
     3781 attttaataa accaagaaaa tatgacaacc tatacaattt tatacacaat agaacaacaa
     3841 taaataacaa cagtaacact cgagttttga ttaattcagc atattttcat attaaatatt
     3901 taaagaaata tgagtatgaa gaagttgtat tagattatgg gctaaataga cagtcatttt
     3961 taatttatgc aataaataac ggaaatttgc caagcaataa aaagaacata attattgaaa
     4021 catatgccta tctggagggg ggatctaaaa aactaattaa gtcatgtaat atagagtatc
     4081 aattatcagt aggagaaatt aaatgtgttt tcgaagaaga tttagaactt gacttaaaaa
     4141 aaatttttgc cagcattcat gattatatag gt
//
LOCUS       DACEMS010000157         4119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEMS010000157 DACEMS010000000
VERSION     DACEMS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.164"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..2376
                     /locus_tag="IW735_002518"
     CDS             4..2376
                     /locus_tag="IW735_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045480.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3906
                     /locus_tag="IW735_002519"
     CDS             2437..3906
                     /locus_tag="IW735_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ1045481.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3917..>4119
                     /locus_tag="IW735_002520"
     CDS             3917..>4119
                     /locus_tag="IW735_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1045482.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWA"
BASE COUNT         1602 a          655 c          902 g          960 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacggggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac ttcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagcg
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gctgaactaa agccgaaaca tgaacggcaa aattttttag ttggagacga ccgtttagat
     2941 catgcggttg cttttttatg gaaagatcca cagacaggag agacagtagg ggcaagttat
     3001 caaggaacga tcgtcgattt taatcgtttc ggtaaacggg gaacgtataa acacattgac
     3061 aagaatccta cgccaaatca cggatttaat ttaaaaatag gcgatccgaa gcatttaaaa
     3121 ttctttgaaa gtagtattga tctgttaagt tacgctgcat tgaatcggga gaaactacaa
     3181 gatgcctggt tagtttcgat ggacgggtta aaacatcatg taatcagtca ttacgttgaa
     3241 gagtcgattt ctgaattaag acgaaaacaa acatttcctc aatcgattga aatatgtgtg
     3301 gacaatgatc gtgcaggaca tattttttat gaaaaagaac agatgaaggg aatcgttgat
     3361 ccttttacca ataagaaaat ccgttgtgag cgaggaattc caaatgattg gcaagtacct
     3421 aaagaataca aagctactta tgaagcagtc gcaaaagaaa tgagtgttga acctgaagca
     3481 attatggcca tacacaaaac tgaaactaat ttacagctga caaatcaatt ggtgtctgct
     3541 catgatgttc aatctacgtt tggaaagatg ttggcgaagg gcgaaccagt agaaacaatt
     3601 gatttgaaag aagcatgtac cacggtcgcg aaggaactga aagtatgtga acgcgcagat
     3661 ggaacataca actttgatcg tttttatagt agaaaagcca acataaaaga cgttaatgca
     3721 ggaatccttc tttcctataa agcagaacaa tactacaaag gctataaaaa gcatgaacat
     3781 gaatttgtac ctgaagtgaa aaaggattgg aatgatcaat tgaagcatga gatccagcaa
     3841 caagaaatca gaaagcaaaa acgtgcgatg ttgtttcaac aaggcagaca acaagaaagg
     3901 gagtgaagtc gtcactatga aaaccgtcat tttagcagaa aaaccgtcac aagcgaaggc
     3961 ttatgccgat agcttttcta aagctacccg aaaagatggg tattttgaga tccaagatcg
     4021 attgtttaca ggagaaactg tgatcacgta tggttttggt catttagtcg agttagattc
     4081 gcccgatatg tacgatgaaa aatggaaaca atgggcatt
//
LOCUS       DACEMS010000158         4057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEMS010000158 DACEMS010000000
VERSION     DACEMS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.165"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..635
                     /locus_tag="IW735_002521"
     CDS             <1..635
                     /locus_tag="IW735_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ1045483.1"
                     /translation="LSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQ
                     AKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEA
                     NLPNILGDKGYISHKIHEKLALMGITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFS
                     LLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            complement(694..2496)
                     /locus_tag="IW735_002522"
     CDS             complement(694..2496)
                     /locus_tag="IW735_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ1045484.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
     gene            complement(2559..3401)
                     /locus_tag="IW735_002523"
     CDS             complement(2559..3401)
                     /locus_tag="IW735_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045485.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            complement(3417..3638)
                     /locus_tag="IW735_002524"
     CDS             complement(3417..3638)
                     /locus_tag="IW735_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1045486.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            complement(3781..>4057)
                     /locus_tag="IW735_002525"
     CDS             complement(3781..>4057)
                     /locus_tag="IW735_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ1045487.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
BASE COUNT         1231 a          819 c          583 g         1424 t
ORIGIN      
        1 gcttaagttc aaacttagct tacactatca agattattcg atactttttc atcaagaagc
       61 tcaccaaagg tgaactagtc ggaattatag atagttttcc tagtccatta tgtaaaccag
      121 ttagaaatag acaagcaaaa ctattgaatc aaatagccaa agttggctat aactcaacaa
      181 aaaaatctta tttttatggt cttaaaattc atatgatcgt tactaagact ggctttccga
      241 ttacttactc aatcacgaat ccaggtgtcc atgacgtgaa agtgttagag actttatcgg
      301 aagaagcgaa tcttcccaat atcctagggg acaaaggata tattagccat aaaatccatg
      361 aaaagcttgc ccttatgggc attactatat ccattccgcc acgtaaaaac atggataaat
      421 cagaaaaact agaccacagc ttacttggaa aacaaagaaa aacagttggg actgtttttt
      481 ctttgttaga aaaattaggt tgtcaaaatt tcaactcacg ttctgttaaa gggctagaga
      541 gtagatttga aagcatatta ctggcttaca gtgttctatt aagtcgagca caacgacgtt
      601 ttgaaggaac tttgagatat tctttaggat attaaaatta actagcacca cgggtatcta
      661 ttaagaagat cctaggttat cttagattat ctgctaatct ttgtatttcc agcagtatcc
      721 tccagcatgt ttttgaactc catttgcagc atccctaata ctttttgagt taacgccaac
      781 ttcttttaca gcagaagcta tagtatcgaa ttcttttatt attgttccat catcagtcat
      841 ttgaataatc tgtcttccac gcttcttgct aggctcacta atcgcatcct cttttttgtt
      901 cgcttttaga aaatcagatt tttccttagc tatccgctct gcctcttgtt tagcaaataa
      961 tagcttaatt gccttatccc tacgagcctc taaagtacca acatcgtgcc gtgttttacg
     1021 cataattcct tcatcaagaa tgagaatttc attatcgtaa tccttaattt ttcgataagc
     1081 tttagcataa gaatcatata gcgcaggagc atcatagccg ttatatcgag ctttatcaaa
     1141 aagtgcaata gcttcatcta ctttgccatt tttcctagca gtttctccac gttcccaata
     1201 tggataacct gcatcaaatc ctttctccca attccctata tttcgtatct tgtttaatga
     1261 aactcgatta tgcaacccgt taataattac atcttctcta gatatcactt tttcaatatt
     1321 taggttaact tcagtctcat catatccacg attttttgca tcgaataata actctttcat
     1381 cgctgatttt gaaatagatt tcagcatact aaggctttgc tctgcttctt ttttagtgta
     1441 attactaaca ctcatatatt cattcataga attataacaa tcgctataag cactgaaaat
     1501 ataatttgaa agaggcgtta agtcaaaggg gctacgaaaa tagactcgca catttgttgt
     1561 accgccttca cttacagtac aaggttctat aggtaaatca attccatttg tagcatcttt
     1621 tctaataatg atgtaatttc ccttcttagc aaacttaaac tttaagaatg tatatagaca
     1681 agttacatcg acataattat tactgttttt tcgatacctg atccaactgg tgtcgcatcc
     1741 ttttttctca ataatatctt gaataaaagc acaagcttca agttctttta tgtctggagt
     1801 catcttttct atttgctttt tttgttcttc caacgactcc tcaataatat ctaaaacacc
     1861 ccaaaaaatc tttccgcata tatctgcatc tgattccgct ctatgggcag tgtcatttat
     1921 taatccgaaa tattccgcta cagtctcttg cttatgattt acaatgcctt ttatatgttt
     1981 tctagagaga ctcagagtat caacatactt aattttagca tcatacccaa gtctagaaaa
     2041 tgtgttactt aaaaatccaa aatcgaaact tgcattgtgt gcacacatag tgatttttcc
     2101 atctagtgca tctcctaaaa attctaccaa ctgggggtaa acttcctgct catttggagc
     2161 agagtttagc atttcattag ttatattatt tattgccgaa gctgaggctg caattctaac
     2221 ttttggattg acaagagttc cgaatgattg aattggaact ccttctttaa atagcaccgc
     2281 accaatctct acaattctat ctgaagaaga atttaggcca gttgtttcca catcaaaagc
     2341 gataaaagtt ttacgaaact catctacatt tgatatgtcc acttccaatt tttcagcaaa
     2401 aactttacca gtacgaaata gactagaagc cagttgataa tcaacgtgtt tactaacctt
     2461 tccttgtttt ttctttagaa aatcaaataa tcccactatt atttcctccc ccattaattt
     2521 tatccagaat cacttttacg atgccttttt cttatattct acctattcgc ataccacaag
     2581 aagtgatcta actttcttct tctaaaagga atatgtatat tattttcatc aagtagtttt
     2641 ccaacacatt cgttaaatgc ctcgtaatca aaacttcttc gccacgtatc aatcttattc
     2701 gtagttagtc cttggatata gtgtggaaga gtatttttaa cgataccatc aaagatggag
     2761 taatcatctc tcccacacac ttccacatta tgatatgtac agtacttgga tgcaaatgag
     2821 aacatattaa ctgcaccaat gttccttgcg ataatattta tcaactgagg atctcctttt
     2881 gccaaccgat catcaaaatt tggaatctcc agaattactt tagccaaatc atatagagac
     2941 aacctgctct tatattgaga aagatgtgtt gaatttgtta catcaattac cgcaatcttc
     3001 atcgcaatag tatttaaatc attatgaaga ggaaatgcat tcaaaacatc atgtattatt
     3061 agaccttctt tcccataatt agagcttgca cctacaaact tatgctgttt ctcaatattt
     3121 tcttcagtta aagataagta tttttgttta tggattgttt cacttatagt tttttcttta
     3181 cttgaatcaa gcttaagaac cttgtcaatg caccaatatg ttgttgctcc ttttattggg
     3241 gaaatatcat tctgcttaag atattctttt aataaactca gttgttttcc ttctggaata
     3301 gtaccagatt tcgtttctat gatatgaggt tgtggtttgg aagtaccttc attagcagca
     3361 aatacagcat actcaacatc atttattgtt atgattttca atacaaatta cacctcctaa
     3421 ttacctatct ttatttcatt gccatcaggc aatataaaag cctgcacaaa cttgacacct
     3481 agcacatcag caatcgcttt caactcatct aaggttaccg tttctcgttg taatttctta
     3541 ttgaaattct gtggagtctg tccaatacgt ctagcaagtt cggaaacact tatattcatt
     3601 tgctcacaca gttctttaat catttctgcc gtagtcatac agcacctcca agtttacgct
     3661 aagaatatta taaatcattt ggtttataat aacaatagtt attaaaatat tattataatc
     3721 agcaagcaaa agaaaaccca cgactctact gtagaatcat gggctattta cttctggttt
     3781 tcatatttcc atttcgatgc cagatttgaa ggatatgacg aagtgttctt catagactgt
     3841 aacgctctgg attatcttcc ttacaagctt atcatcgtac tctaaggtac ggaatttgtt
     3901 cttgcggata aattcaatca gctcattgat tcgctcgttc tcgccactga gggaggcatc
     3961 ttctactaaa aggagctgtc gcttgtctct cagctcatca atctcatctg ctagatgttc
     4021 ataatcttga cccttattag ccagcttgat gagttct
//
LOCUS       DACEMS010000159         4035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEMS010000159 DACEMS010000000
VERSION     DACEMS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.166"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            178..774
                     /locus_tag="IW735_002526"
     CDS             178..774
                     /locus_tag="IW735_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1045488.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="IW735_002527"
     CDS             898..2697
                     /locus_tag="IW735_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1045489.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="IW735_002528"
     CDS             complement(2975..3187)
                     /locus_tag="IW735_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045490.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1185 a          688 c          712 g         1450 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat attta
//
LOCUS       DACEMS010000160         4015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEMS010000160 DACEMS010000000
VERSION     DACEMS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.167"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(176..1462)
                     /locus_tag="IW735_002529"
     CDS             complement(176..1462)
                     /locus_tag="IW735_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1045491.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IW735_002530"
     CDS             complement(1455..2723)
                     /locus_tag="IW735_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1045492.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IW735_002531"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IW735_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1045493.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IW735_002532"
     CDS             complement(3495..3941)
                     /locus_tag="IW735_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1045494.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1137 a          816 c          707 g         1355 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaat
//
LOCUS       DACEMS010000161         3925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEMS010000161 DACEMS010000000
VERSION     DACEMS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.168"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(573..1130)
                     /locus_tag="IW735_002533"
     CDS             complement(573..1130)
                     /locus_tag="IW735_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045495.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IW735_002534"
     CDS             complement(1309..1920)
                     /locus_tag="IW735_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045496.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IW735_002535"
     CDS             complement(2033..2290)
                     /locus_tag="IW735_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045497.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IW735_002536"
     CDS             complement(2887..3165)
                     /locus_tag="IW735_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045498.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IW735_002537"
     CDS             complement(3199..3450)
                     /locus_tag="IW735_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045499.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DACEMS010000162         3897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEMS010000162 DACEMS010000000
VERSION     DACEMS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.169"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..300
                     /locus_tag="IW735_002538"
     CDS             <1..300
                     /locus_tag="IW735_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1045500.1"
                     /translation="NEDIFVIKRRYFTQASDELIHAFSNEWFVSEKELHASNLQYMPG
                     EDPIPNMKAIINSKDYEGYKAKHPKAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            415..2187
                     /locus_tag="IW735_002539"
     CDS             415..2187
                     /locus_tag="IW735_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1045501.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            2162..3454
                     /locus_tag="IW735_002540"
     CDS             2162..3454
                     /locus_tag="IW735_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HAQ1045502.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGYKKIGILSG
                     LGGEIFTYHFFIPQAASSYLEFVEQMREVEAALQTTFQ"
BASE COUNT         1483 a          576 c          710 g         1128 t
ORIGIN      
        1 aatgaagata tttttgtcat taaacgtcgc tattttacac aagcaagtga tgaattgatt
       61 catgcctttt ccaatgagtg gtttgtttct gagaaagaat tacatgcttc taatctgcaa
      121 tatatgccag gtgaagatcc aattcctaat atgaaagcaa ttatcaacag taaggattac
      181 gaaggatata aagccaaaca tccaaaagcg aagccattta aatacccaca agaaatgaag
      241 cgagcctggc gtaaaatgtt agatgatgag ttgattccac ttgagaatga attgaggtaa
      301 acgagaaaat tgaagaaatt gtatgataaa catgttaata aatgatgctc gaaataaaat
      361 ttggttttat ttttacattg atacggtaaa taaaatttga aagtagaaga aaatatgaac
      421 aacaatgaaa caaaaaaaac atatgaacta agaataactg ctgatgagat taaatcaaca
      481 gacccaacat ttactatgaa gaatctaagc ttaggggata attatcaacc aaaagatgct
      541 tacttccgta cggcagaagg cgaaataact agaagaaact acggtaaaaa tgaacggaaa
      601 atttcaaatc aaacattacc gcgattagct attcaaatgt ttgaacaatc gattgtttct
      661 ctaccagaaa gcgaaagagc aacatttcct gtgtgtcgat atcactcagg agatgagata
      721 atacgaggga tttttccaga tgttgacagt ttcaaaaaaa aaaggaagtc cattgaattt
      781 ttgatttata aatataatga agacaagcag atagtcatct attgttggag tattttttca
      841 actttattat ttgtacaaga atgtttgaaa cgttttggaa catctgggga tgaatttatt
      901 cttgtttata aagggaaaga gaaaaaggaa atacgagtag gagagactca gactatgacc
      961 aatcatttgc tgaaatcata tgttttacat ttactcaaat ctaaaaacct tattctacgt
     1021 ggcgctcctg gaacagggaa aacattcctt gcaaatgaaa tggcagctga tgtcgtttca
     1081 aatggcagaa ctacacaaat caatgaattg agcgaagatg aaaagagccg aattggattt
     1141 gtccagtttc atcctagcta tgattatact gattttgtcg aaggtttacg tccaactaca
     1201 tccgatgatg gcgttgttag tttcaccttg aaagatggaa gctttaagag ctttgttgaa
     1261 aaagcaaaga aaacaaagaa agtaaatgga caagataatt ttgaagaagc ttgggctaaa
     1321 tttttcgatg cggtaacgga agctagtata gagggagaag gctacaataa acttaaaaca
     1381 ttgacaggta aaccagtacg caatcttcta gcctatgaaa gaaatgaaat tcaaggtgtt
     1441 tatcctgcta aaaccaatac ttatctaaac catgaccaaa tttataatgt ctatcgtgga
     1501 ttgccaggta cgccaaaagg tggatttgat gcttatcgaa aagcgattgt aaaacattta
     1561 aaagaagaat ttggcttaaa aaactacacg gcgtcaacgg tagattctac gaataaccaa
     1621 aactatgtgt ttatcattga tgaaattaat cggggcgaaa tttccaaaat ttttggcgaa
     1681 ttatttttct caattgatcc aggatatcgt gggcataaag aaggtgttta cactcagtat
     1741 gcaaacctcc atgaaaatcc agaagaaaaa ttctatattc ctaataatgt ttatattatt
     1801 gggacgatga acgacattga ccgttctgtt gacacttttg attttgctat gcgccggcga
     1861 tttactttct tagaaataaa agcagaagat tcagcacaaa atatgctgac tcaagagaaa
     1921 acaaaagaac ttatgacgcg tttaaatgag gcgatcgttg ccaaagaaaa aggtggattg
     1981 tcaactgatt accaaattgg tgctagttac tttttaagta tcgataatgg tgaggagaca
     2041 atggaacaat tatgggatac taagttatat ccattattga aagactattt tcatggtgaa
     2101 cataaggcga aagaaaaatt agaaatacta gaaaaggtat actctaatga cggtgattta
     2161 gatgaagctg aaacctctac acgataatca agaaatcaat gactcagttg tgcaacatcc
     2221 aatcattacg aacttagtta atcgtgactt aagtagttta gagaaagaaa attttattat
     2281 ctttccgcaa cagttaaatc aatccaagga tctaagtgaa gataacttga tatttcaatc
     2341 aagaaatgga aaaatttgga cttgtaatgt cgtaggtatc cttagcgatt caacagatga
     2401 attaagaatt cattctagat tcgctaatca aatgaagcat gaagatttct ttttacgtta
     2461 tatgcttcag cgtgttttga attacaacgt gattaatgat cattttagtg catctaaaaa
     2521 gatgacatac tacgatttgt tggtatttct ctttccttat tatctgaatg aagcaatgcg
     2581 taaagggaca tacaaagaat atgtcaaacg agaatacaat aatgccaata tcaagggagc
     2641 tgttgatgtt gcaaaacaca tccgttcgaa tgttcctttc gttggaaaag ttgcttatcg
     2701 aacacgagag tttagctata ataatcacct aacccagctt attcgtcata ctattgaaaa
     2761 aatccaaaat gagtacgatt tcttacttag tggagatgag gatacgaaag aaaacgtggt
     2821 tctaatcaaa caaacaacac ctggttatgc tagattaaat caatttaata tccttcaaga
     2881 gaatatcctt catccagtca aacatagtta ttacgaggag tattcggctc ttcaacaaat
     2941 ctgtatccaa attttatcgg aagaaaaatc aggatttgga agtgataaaa atcaaataca
     3001 tggaatcatt atcgatgtgt cttggctatg ggaagaatac attggaaaag tcactggttg
     3061 gaaacattat ggcagagata aagggttagc aacaatgcat ttatttcaag agcccaaccg
     3121 gtccccacga tatccagatt ttacatttaa taatatacca attgatacaa aatacaaaaa
     3181 acatcttgat actagaaatg actataatca gttagtcaca tatattcata taatgaattt
     3241 agaccaagca gatacaatga aaggcggctt tctgcaacct acttccgatc cccttcctgc
     3301 aaatcatggg tataaaaaaa taggcatatt aagcgggcta ggaggagaaa tattcacata
     3361 tcattttttc attccgcaag ctgcgtcaag ttatttggaa tttgtagaac agatgagaga
     3421 ggtagaagca gcactacaaa ctacttttca ataagtagga attatagaaa ttttactcaa
     3481 aaaatcttca ttaaaaaatc tgactaaata agttgagaaa acagaatata cttcaatatc
     3541 cagtcattta taaatatcat tcactcatga tacccagctc cgtatcacaa ataatgatat
     3601 tttttaagag aaaaaattat ccctatttta aataatagca ataggtagtg gtagagagaa
     3661 atttataaat aactttgtta tttgctttgg tagccaagta agttgcaaaa gaaaaataga
     3721 tactgttaaa aagataatgt agacatagaa acaaatacgt aaaattttag ttagaatgat
     3781 ggcaaggaga aaaaacatga tagatgaaaa aaagtgttta aaaaatctat tggaagatat
     3841 ggatgatatt gaagaaaaac tatctaaatg gactgggaaa acaaatatat ttcagaa
//
LOCUS       DACEMS010000163         3883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEMS010000163 DACEMS010000000
VERSION     DACEMS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.170"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..3123
                     /locus_tag="IW735_002541"
     CDS             82..3123
                     /locus_tag="IW735_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1045503.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..>3883
                     /locus_tag="IW735_002542"
     CDS             3313..>3883
                     /locus_tag="IW735_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAQ1045504.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1329 a          621 c          824 g         1109 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gta
//
LOCUS       DACEMS010000164         3810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEMS010000164 DACEMS010000000
VERSION     DACEMS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.171"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..2193
                     /locus_tag="IW735_002543"
     CDS             94..2193
                     /locus_tag="IW735_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1045505.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2389..3252
                     /locus_tag="IW735_002544"
     CDS             2389..3252
                     /locus_tag="IW735_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1045506.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3357..>3810
                     /locus_tag="IW735_002545"
     CDS             3357..>3810
                     /locus_tag="IW735_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1045507.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1524 a          636 c          772 g          878 t
ORIGIN      
        1 agtaaatcct aaaaataagc tattggcgtg acgtatagaa tatgatagat taaatggtag
       61 cgtaaaccta gtaaaggagc cgatcgctaa gtgatgaaaa aatgtccaaa ttgcggatcg
      121 aaaaaaatgg agcagaatac tatctgtcca gactgcggtc atcaaataac tgcagaagat
      181 cagcaaataa aagaatcaat tgaagaaaca aatacagatg cagctatgca ggataaatca
      241 actgaagatg cagtctttcc tgatacagaa ttaaacgatc caatcgaatg gtcggaattg
      301 aaagatttac ctctagaatc tgtaatggaa ttattcgaaa aagcagaacc agaagacgaa
      361 gaaccacctg ctcacgatca agtaactaat atagagaaaa atgaacagaa gaaagcaact
      421 gttcatgaga cttcggaaga atcaggaaag aatcaagaag aaacgcttga atcaaaacaa
      481 aaaagaagag tagctgaact gaaagaaatc gtcgataatg aggaagaaaa ttcgatacta
      541 tccgcttata tcaaagcaca tcgtgaagat acaaaagaag agcacgcgaa agagctgctc
      601 aaaatgatca gtgaaaaaat tgcacaggaa aacaaagtag agccagaaaa ggaacaagaa
      661 actgaaaaag actctgatag agaaaacgcc acaggtaaag aaacagtaaa aataaaagac
      721 gtaaaaacgg atatcacaga aaaaactgaa gtatttactg aaaaacaatt agaaaaggaa
      781 aaaacgaata aactgccgga agcagagaaa aaagaaatac tcgaaaacac agacaaaaag
      841 tcaacagagc ccgatgaaat aaatagtgaa tccgatgctg aaaatcaatc tgtaagcaaa
      901 gagggtactg atcttgaagc aaaaatagaa aaagaagaga accagcctaa gctgtttgaa
      961 gcagaagcga aatcaataac aatcgtgcca gatactgaaa aaacacatca agaatcaaac
     1021 ttagaaaaaa cagatccaga taaagcgcaa aagaaaaaat caaaaaaagt accttatctc
     1081 gttttagcag cagtacttct actcggtgga ggaagctggg cttattatga ccatcatcaa
     1141 aaagtagaag ccgaagtagc agcagaagca aaaagacagc aacaaatgga agaacttcat
     1201 gagaaacttg ctgcctttta tacagatgat caccatcaat ttattcgtgc agatatgatt
     1261 catcaagatt tatcaaaact aaaaaatgat ttatccagtc ttaagaatga aaaaggatat
     1321 tcagagttag aaacaactta ccaagatatc caatctaaga tccgagatat ccaaaaagtg
     1381 aatgagtggt tcatagcacc tgccatcgtc gatgatcatc tagcagatga catcaagttg
     1441 aaagcagata aagagatcca agcaatcgaa acggataaca cagattttgg aaaattgatt
     1501 caacaagcac aaacagaagc taaaaatcaa tatgctgaat tgcaaacagc taaagaaaaa
     1561 acagctatag tctttaaaga cggaaaagta gtggattcag caacacgcga ccaatacaag
     1621 acggcaaaag aagcagtaga aaaagtgaaa aatacagatc tatctgctca gttgaatgaa
     1681 caattgaaac aagtagataa gatgctcact gataaagaga aaaaagaaga agaggaaaag
     1741 aaaaaagcag aagaagcgaa aaaagcagct gaagaacaag caaaacaagc cgctcaagca
     1801 gcagctgcag ctcaagccgc tcaacaagct gcggtcgcac aagcagcaca gcaagaagcg
     1861 gcatcctcga ccccttcctc ttcaaactca gcgaatcagc caatcaaatc tgccagagca
     1921 agtgatattg ccgatgcatc taatccagca tggacgtggg caccaggtgt ccaaagcaaa
     1981 gtgattgcta catgtatcca acgtggatat atcgttgaag gaggttacag attagaaaaa
     2041 gccagaatcg aaaatgggga aggctattat aatctatatg ccacttcaac aaaatctgca
     2101 ttgatgaaag gcattggaga aagtgctttg ccattttaca tcgtaaccat caactgtaaa
     2161 actggatggt tcggaggaaa tgggagcaat taattttagg caaatgaaag aggctgtgag
     2221 cctgccgttt aacaccgttt attcgaaaaa ggggagtatg acacaactgt tgtcatactc
     2281 cttttttcac ctttccaaac caaggctcag ctattcaagt agataacatt atgttatgat
     2341 agcaaagaaa tgtattaaga aaacctttca ctatagaggg agaaaaaaat gaacgattta
     2401 ttagcaacta tatttacagg tattattatt gatgaaaatg aaaaaaacta ctttgtccaa
     2461 aaaaacggac aaacctttgg cttaagcaaa tcagaaggtg aacacaaaat cggtgaagca
     2521 gttgaaggat tcggttacca aaatcaaaaa catgaaaatc ggatcactac acaaattcct
     2581 aaaagccgta tcggtcatta cgcttttgga atcgttactg ctacacgaaa agatttaggt
     2641 gcgtttgtcg atatcggtct tccagataaa gactttgtcg tatctcttga tgaattgccg
     2701 acaatgacag agctatggcc taaaaaaggg gatcgcttga tgatttccct tagagttgat
     2761 gcaaaagacc gtatttgggg cagtttagca gatgaaaaaa tattcaaatc tttaagcaaa
     2821 tatggaacag aagaaatgaa aaacaaagat gtgataggaa cagcataccg cttgaaatta
     2881 acaggtacgt acgttttaac ggaagatttc tatcttgggt ttatccatcc ttctgaacga
     2941 tataatgaac ctcgtctagg cgagcagata aaaggtcgag tgattggtgt ccgaccagac
     3001 ggtgtactga atctttcatt gaagccaaga gcatatgaag ctatatcaga tgatgcacaa
     3061 atgattttaa ccattttaga acgttctgca gatcagcaaa tcaactttac agataaatcg
     3121 gatccagaag aaatcatgaa agcttttggt atcagcaaag gagcatttaa gcgcgcatta
     3181 ggtaatcttt tgaaacaagg attgattaca caagaaaacg gtgtgacaaa actagtcaaa
     3241 aaatctaatt gagaatccct tgcatgtttt tcttaactag cttataataa ttttaattaa
     3301 catagtgtta tatctttttt tatttgtaat tattataaat tgttggggtt gagtaaatgg
     3361 tcatgaatgc tatttcggcg atgaaaaaaa caaaacagca gctacatgaa gcggggttta
     3421 agctgacacc gcaaagagaa gctactctgc ttgtgctttt ggaaaatgag aaagagcatt
     3481 tatctgcaga agaaatcttc tttttagtga aaaaaaagag tccggaaatt ggacttgcta
     3541 ctgtttatcg aacccttgaa atcttgacag aattgaaaat tgtcgataaa gtaagtttta
     3601 atgatggatt agcccgttat gatttgagaa aagaaggcgc caaacatttc catcatcatt
     3661 tactatgctt gaactgcggc acgatcgagg aagtagaaga ggatctttta cttgaagtgg
     3721 agcaagtagt agaagaacgt tatcattttt tagtaaaaga tcatcggcta acgttccatg
     3781 gaatctgtca ggaatgctat gaaaaagaaa
//
LOCUS       DACEMS010000165         3543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEMS010000165 DACEMS010000000
VERSION     DACEMS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.172"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(560..1600)
                     /locus_tag="IW735_002546"
     CDS             complement(560..1600)
                     /locus_tag="IW735_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1045508.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="IW735_002547"
     CDS             complement(1949..2275)
                     /locus_tag="IW735_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045509.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="IW735_002548"
     CDS             complement(2262..3050)
                     /locus_tag="IW735_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1045510.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1084 a          584 c          487 g         1388 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aac
//
LOCUS       DACEMS010000166         3387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEMS010000166 DACEMS010000000
VERSION     DACEMS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.173"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..530)
                     /locus_tag="IW735_002549"
     CDS             complement(27..530)
                     /locus_tag="IW735_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1045511.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
     gene            complement(653..1417)
                     /locus_tag="IW735_002550"
     CDS             complement(653..1417)
                     /locus_tag="IW735_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1045512.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1525..1800
                     /locus_tag="IW735_002551"
     CDS             1525..1800
                     /locus_tag="IW735_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1045513.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            2238..3188
                     /gene="yidC"
                     /locus_tag="IW735_002552"
     CDS             2238..3188
                     /gene="yidC"
                     /locus_tag="IW735_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1045514.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         1010 a          707 c          644 g         1026 t
ORIGIN      
        1 atatggtcta ttattggttt tatctattaa atcgaggatt ctttcccaaa gtaacgttgc
       61 catctcttac gcatacttgg tgacataggc tgagcggctt ctttgactgc gcagtatttg
      121 tctacgaatg ttacgatata tccttctttg tacttaggcg gtgcaatcgt tgcgccccac
      181 atgtgcttca aaatgatatc tcgctcaaga tccgacaatt cggtgatctt ttctgcattc
      241 tttacagcaa ttcttggatg gatatacgca tgtgtgcctt catcaaattt cgtcgtacgc
      301 caatcatagt agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca
      361 ccccattttt ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga
      421 gtggaatgaa catgctgtgt gtaattcgct aattttttca ctgcttctgt ttctaaaaga
      481 tcttctacat aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat
      541 ctgccacctt tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc
      601 ggattattct agtcgccttg gataaaatag ccaaagctta atagttcctt aatcataaag
      661 aaaaatgata aagaacaatc cctgccgcaa ccccaacatt caacgattcc gcatttcctt
      721 tcatcgggat atatacgttc tgatcagtca aagacaagat ttcctgactt accccctgac
      781 cttcgttccc catgattatt gcgaagttat ctgatttttc tactttgtct aatgcgtgtg
      841 cttcttcatt taattctgtg ccatacactg taaacccttt gtctttcatt aaaggaatca
      901 cttctgtcaa atctttttgc aacacaggca aatgataatt gcttccctgc atgctgcgca
      961 gcacctttgt actataaata tctgccgttc ctttgcctaa taaaacacca gccatatcaa
     1021 atgcatctgc cgtacggatc atcgtcccta catttccagg atcttgtaca ttatctaaca
     1081 acagccatcc tcctgaaaaa tctggcgtgc cttcagccgt taacgccatc actgctaaga
     1141 ttccttgtgg tgtaggtaga tccgatacag catccatgac ctcatccgaa actagaaaag
     1201 tagaaatctc tttgtttgcg gccagccagt ctccccattc tttcatgcct cgctgtgtca
     1261 caaaaagttc ttttatttct gcatcagacg acgcagcttc ttcaatcaga tggaaccctt
     1321 ccaaaagata ttcttccttt tcttcccgat atttcttttt atgtagtttt tttaattctt
     1381 tgattcttgg attttttgct gattggatct ctttcacatt cctcacctcc ccatgattat
     1441 atcatggaat aggtgcttga gcttgtttca tcctaaaaaa atcggtatca ttaatttata
     1501 aaataggaaa gaaaggtgac gaacatgcgc aaagttaaaa tgaacgtcca aggacgcgtc
     1561 caaggcgtag gatttcgcta catgaccaag atggtcgctg atcaactagg cgtgactgga
     1621 acggtaaaaa acgaagatga cggctcagtc acgatcgaag caatcggcaa tgacgacatc
     1681 atccagaaat ttattgaaga agtcaagaaa tctccgagcc caagtggacg agtgcagtat
     1741 gtggatgtcc aagaagaccc gatgattgag gaacgaaaga aatttgatgt ggtgggataa
     1801 ggttgtgaaa aagctggtct gcatcaagca ttaaggacag gttgtgaaaa atagctctcc
     1861 atatttttca acaggctgga cttaatgccg tagatgcaag cttttgaaca ccgtcgataa
     1921 ggttattctt gaggcgcttt gcctcaaaaa ttaaggacag cagtgaaaaa gctgatctat
     1981 cacttgcagt gagttcattt gactccgctt tttcgggcag aaaaaaagtt tttagtttat
     2041 taaagtaaga aagaaacact tggaatcatt ctactttttc ctagtatttc tttcttgatt
     2101 ttttgtcttt ttttcaaaat ttttcgctac gtaaaagaac gaatatttat atgaaatttc
     2161 tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg taaaaattat
     2221 agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc ggacttgtca
     2281 gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac cctgacactt
     2341 cgggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca tatctagttg
     2401 aaaatttcaa ttggtcttat ggctgggcag ttattgcaat gactgtgatc gtccgtatca
     2461 tcatcttgcc actaggtatt agtcaatcga aaaaaacaat gatccaatct gaaaaaatgc
     2521 aagcgttaaa accacaagtt gaagcagccc aacaaaaatt gaaagctgca acaacacgtg
     2581 aagaacaaat ggcagcacaa gcagaaatgc agcaagtata ccgcgaaaac ggattgagta
     2641 tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc tctgccttgt
     2701 actttacggc acgttataca gaaggtatcc gggaatcatc tttctacggc atcgatttag
     2761 gctcaccaag tatggtactt gttgccattg caggggtcgc ttatctgctt caaggataca
     2821 tctcaacgat cggtattcct gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct
     2881 ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca ctttactggg
     2941 tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg atgaaaccac
     3001 gtttgaaagc acaagtggca gaagagttga aacaaaaccc accaaaacaa gtcgtcactc
     3061 caagaattga tgtgacagaa tcagctaaac cagctgacag caaacaagca accaaacaaa
     3121 tcaagcaacc caaaaacagt acaaacaata aaggccgcaa cgcaggaaag cagcagaagc
     3181 ggaagtagat agtaaataag agtgtaataa aaattcatta acagttttag caattaaaaa
     3241 tatcgaccaa gatttttgaa aaagaaatct ggtcgatatt tttatttaag tgaatgttta
     3301 atttgaatca tcgattgatc gctatctaat tgcagcagca tctttttatg aagaaacaac
     3361 tttttcttat ctatcaacgt agacagg
//
LOCUS       DACEMS010000167         3373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEMS010000167 DACEMS010000000
VERSION     DACEMS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.174"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW735_002553"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW735_002553"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1045515.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IW735_002554"
     CDS             complement(2260..2511)
                     /locus_tag="IW735_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1045516.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IW735_002555"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IW735_002555"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1045517.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          739 c          626 g         1088 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttt
//
LOCUS       DACEMS010000168         3338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEMS010000168 DACEMS010000000
VERSION     DACEMS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.175"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            116..976
                     /locus_tag="IW735_002556"
                     /pseudo
     CDS             116..976
                     /locus_tag="IW735_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="IW735_002557"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="IW735_002557"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ1045518.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="IW735_002558"
     CDS             1830..2678
                     /locus_tag="IW735_002558"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ1045519.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="IW735_002559"
     CDS             2688..3083
                     /locus_tag="IW735_002559"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ1045520.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT         1303 a          348 c          665 g         1022 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tattaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttatg tgaatcggct acactagact agacagaaaa
     3241 aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgtca agacgtcaac
     3301 gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtc
//
LOCUS       DACEMS010000169         3295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEMS010000169 DACEMS010000000
VERSION     DACEMS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.176"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            40..591
                     /locus_tag="IW735_002560"
     CDS             40..591
                     /locus_tag="IW735_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ1045521.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1267..1533
                     /locus_tag="IW735_002561"
     CDS             1267..1533
                     /locus_tag="IW735_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045522.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1591..2103
                     /locus_tag="IW735_002562"
     CDS             1591..2103
                     /locus_tag="IW735_002562"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1045523.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2100..3137
                     /locus_tag="IW735_002563"
     CDS             2100..3137
                     /locus_tag="IW735_002563"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1045524.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1215 a          555 c          654 g          871 t
ORIGIN      
        1 aagagcgagc aagacaggaa cgagagaaca aacatactca tgaaaagact agaggcttcg
       61 acctcgaact ttaataataa gttagatgtt tttgaagaaa agactaagca tgcaagatta
      121 aactttgaaa caactgcaaa gcactatatt aaacgacttg atgaagataa tttgaaatta
      181 gattttcaac aagctattca agatgaatta agcgatacta aagacgaaat acgtgaagtg
      241 acgaaacaag cgagagaaga aacaaaagag tataaagaaa tcttagaaag taaaataaaa
      301 gaccataaca aagtggttga taaatcgaat acagcgctaa aagtgatgac aaaaggcgtt
      361 acaaatatat tttttgtgct aattattttt gtacttgtaa tgcttgtgac aggtccgata
      421 ggtcatttct ttggtattga acatttatat agctttatta atggttttat tgatgaccac
      481 gaaagtgcat ggcgatattt gatgttgata ctttatgcag taccctatgt attcttcgct
      541 atcattctgt ggctcatagc attgtttttt agagtattta atgaccttta ggtagattta
      601 tatataaaaa gggctaaata cgcaaaatac ccccttaaaa tgcaaaataa atgcaaataa
      661 gggggtaact ccttatgaaa aaacaactaa tagtattata acacgttttt atgccgagaa
      721 aacttattgg ttggaatggg ctatgtgtta gctaacttgt tagcgagttg gttggacttg
      781 aattgggatt aatcccaaga aagtaccaac tcaacaacac ataaagccct gtaggttccg
      841 accaataagg aaattggaat aaagcaacga aaggagttga gtaaaatgag tgagaaaacg
      901 aagaaaatca ttcgagtatc attagctttt tcacaagtag tagtgcaatc attgatagtt
      961 tattatactt accagcaata taaattatct aaagagaatt aagttttgat tatgtaataa
     1021 gttttggtac taaaaaagac ttgatctaat tagaccaagt cttttgttag tgttatatta
     1081 ataacaaaat aaaaataaga agtcgctcac tccccgacca aagtttgtga gcaaaataaa
     1141 cagtcgatgt ttgtttctta attgtatatc ttgatattaa acgatttaag attattcttc
     1201 aagatatata ttcgcaaatg taattggaaa agcgacttct taaacaaaat taaggagtcg
     1261 ctttttatgg caaaaataac gaaaaatgag caagaaaatt tggtggaaaa ttacacaaaa
     1321 aaaccggcgt actctaatag ccggttagac gcacatttcg tgtgcacttc taatccgaaa
     1381 ttgagctttg acgctatgac gatagtcgga aaccttaata aaaacagcgc taaaaagcta
     1441 tccgatttta tgagtttaga tccacaaatt agactttggg atatacttca aacaaaattt
     1501 aaagctaaag cactacaaga ccccctatgt taggctagtt gtcgtatagg ataataagtt
     1561 ataataaaga ctatacggag gatgattacc atgacaaata aaaacaatga acgttactct
     1621 accgaactga aagcgacagc catcaagaaa atgatgccac ccgaaaatag ggggattcgc
     1681 caattaagtc aaactttggg cattccagaa gcgactcttt gttcttggcg taaaaaagct
     1741 agaattcaag ggaatactac acccggaacg aaaaaaaatg tcgaaaaatg gtcaagtgaa
     1801 gataaatttt taattgtgat ggaatcattc tcaatgaacc aagtagaact tgcggaatat
     1861 tgccgaaaga agggcttatt taaagaacaa attgaggctt ggaagaaaac gtgcttatct
     1921 gcgaatgaac aggaagaaaa tcgaacgaga gaacttgcaa ctgaactaaa agaagagaaa
     1981 aaacaagcgc gtcaattaga gaaagaatta cgtcataagg aaaaagcatt agcggaagcc
     2041 gctgctctct tattactaag aaaaaaagca cacgcgattt ggggggacca agaggaagaa
     2101 tgatttcatc ttcagatcgc aaacaagctg ttgaacttat tcaagaagcc aatcgcaatg
     2161 gggcccgtct cacctatgcc tgtaatgagc tgaatattag cgttcgaacc tacgaacgat
     2221 ggacgcgtga aggtacgata gcgcatgatc aacgcccttt ggcgaaacgt cctgtaccca
     2281 aaaataaact gacggatcaa gaacgcgaaa aaataataga aaccgtttca aaaaaagaat
     2341 ttatgaattt gccacctagc caaattgtac caaaacttgc ggattgctcg atttatattg
     2401 catcagagtc tagcttctat cgtgtattgc gagaaaaaaa tatgcaacat caccgtggcc
     2461 gaagtcaagt ttcacaaaaa cgcataccgc caagtcacct tgcgacgaag ccaaatgaag
     2521 tttggacttg ggatattact tggctaaaag gtccaataaa aggactcttt taccggttat
     2581 atctcatcat tgatttattt agccgtaaaa tggtaggctg ggagatttgg gaatcagaag
     2641 aagctcgtca tgccgaaaaa cttattaaac agacggtgct taaggaacgt atccaaggac
     2701 agccgttggt tttacactcc gataatggca gccctatgaa agccgcgact tttcaagtct
     2761 tactcgaaaa attaggaatt caaagttctt attcaagacc acgagtgagt aatgacaatc
     2821 cttattcgga agcggctttc cgaacgttaa aatatcgacc agaatatccc aataacggct
     2881 ttaaaacaat agatgatgct cgaaaatgga cgaaagagtt tgtcgattgg tataacgatt
     2941 ctcatttgca tagtgggatt caatttgtgt ctcctgcagc gtgtcatgaa ggacgacatc
     3001 tcgctatttt agagaaacga aagcaggttt acgaggacgc taaaaagcgc catcctgaac
     3061 gctggtcaaa aggtgttcga aattggactc cacatcaatc ggtggcgttg aatcctttaa
     3121 aagaaacaga aagttaacag gagcataatc tttcaagatt atcaaataac cctcgtcacc
     3181 caatagggga aaaagttatt cttcccctat tggataacga ggccaaccga ccatagggag
     3241 gttctgcttt ttgataggtg gaaaataaaa atacgacaac tatattgaca aacac
//
LOCUS       DACEMS010000170         3295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEMS010000170 DACEMS010000000
VERSION     DACEMS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.177"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            135..1103
                     /locus_tag="IW735_002564"
     CDS             135..1103
                     /locus_tag="IW735_002564"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1045525.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1420..1944)
                     /locus_tag="IW735_002565"
     CDS             complement(1420..1944)
                     /locus_tag="IW735_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1045526.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2231..>3295
                     /gene="scm"
                     /locus_tag="IW735_002566"
     CDS             2231..>3295
                     /gene="scm"
                     /locus_tag="IW735_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1045527.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         1049 a          667 c          611 g          968 t
ORIGIN      
        1 aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca
       61 accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa
      121 aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag
      181 attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga
      241 aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc
      301 gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta
      361 ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac
      421 tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag
      481 ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc
      541 tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca
      601 gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca
      661 atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata
      721 tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc
      781 aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag
      841 ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt
      901 ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg
      961 tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg
     1021 gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc
     1081 tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca
     1141 gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc
     1201 tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa
     1261 atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc
     1321 cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt
     1381 gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa
     1441 acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag
     1501 acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga
     1561 aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag
     1621 cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc
     1681 ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat
     1741 tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc
     1801 cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc
     1861 ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc
     1921 gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt
     1981 tcctaattct aggataccta cttatatgtt actacacacc cttatttttt acactttaac
     2041 agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg
     2101 aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca
     2161 aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg
     2221 aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat
     2281 cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat
     2341 ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt
     2401 tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact
     2461 tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa
     2521 ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag
     2581 aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac
     2641 agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg
     2701 gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct
     2761 tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg
     2821 gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga
     2881 agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt
     2941 taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg
     3001 ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga
     3061 gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac
     3121 tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc
     3181 agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac
     3241 agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt catca
//
LOCUS       DACEMS010000171         3282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEMS010000171 DACEMS010000000
VERSION     DACEMS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.178"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            647..964
                     /locus_tag="IW735_002567"
     CDS             647..964
                     /locus_tag="IW735_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1045528.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IW735_002568"
     CDS             complement(1055..1420)
                     /locus_tag="IW735_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045529.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IW735_002569"
     CDS             1628..2110
                     /locus_tag="IW735_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045530.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IW735_002570"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IW735_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1045531.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1137 a          499 c          598 g         1048 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tg
//
LOCUS       DACEMS010000172         3270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEMS010000172 DACEMS010000000
VERSION     DACEMS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.179"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..238
                     /locus_tag="IW735_002571"
     CDS             <1..238
                     /locus_tag="IW735_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045532.1"
                     /translation="SNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQE
                     VKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            252..440
                     /locus_tag="IW735_002572"
     CDS             252..440
                     /locus_tag="IW735_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045533.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            452..1063
                     /locus_tag="IW735_002573"
     CDS             452..1063
                     /locus_tag="IW735_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1045534.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            1048..2439
                     /locus_tag="IW735_002574"
     CDS             1048..2439
                     /locus_tag="IW735_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1045535.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            2655..2957
                     /locus_tag="IW735_002575"
     CDS             2655..2957
                     /locus_tag="IW735_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045536.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            2950..>3270
                     /locus_tag="IW735_002576"
     CDS             2950..>3270
                     /locus_tag="IW735_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045537.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENK"
BASE COUNT         1287 a          456 c          634 g          893 t
ORIGIN      
        1 gtcaaatcaa ccaagaacgg tagaagtacg tgaaacaaga gctgaagcaa gtcagggaga
       61 taagacgcaa aaaataaata ttatcgagga acgaaaagaa aggcctaacg aaaaagtgat
      121 tgtagaacaa gaagtgaagg agaagaaacg tccacgattt acttatgaaa ataatccact
      181 gtttcaggat atttctgata aaccaaatcc gctgtttgat agtctattta aaaaataaag
      241 aggtgattgt catgcaatta attctacaga ttagtacgca ggatttctta gaacttgccg
      301 aattatgcga ctcagtaaag aaagcgacaa atgaaaaaat cacggtcaca aattatattg
      361 aactgatgat tcaagaagaa ttaatgatga aaaatgaaat agagagcaat ttcaaatgtt
      421 gctctctaga aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa
      481 aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact
      541 gacattgatt gcaaaacaaa gaggaatttc tagagcagat gtttgtcgtg agcggttaaa
      601 gggactcaaa tatgaaccgc cgttattggc tgaagatgat agggtagctc taatcaaaca
      661 gttaaatcaa attggtaaca atttgaatca agcggtaaaa gcattaaata tcattgctaa
      721 atattttaga gaacaatctg aaaataagaa agaaaagagc ctgcatttta gttttgacgt
      781 tagccgttac aacattttta aagatgaact tgcgaatagc atagcaaaaa aaatgatgat
      841 tagttatcca gatgacattt ttttggagtc caatcggagt acatcttctt ttctcaaact
      901 gtcagaagta gaaaaagatt attttcaata ttggttagaa aagcaaacta gacccgatac
      961 gaaagaagag tattcccaaa aagttgaccg gaatttaatt cactcgattc aaattattgc
     1021 aagtttaaaa gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag
     1081 aaaaatttat cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgcgtt
     1141 gcagtctcag gaataaattg tagtgataat agtgaggaag cattgcaaga aatgaattta
     1201 gtaagaacca ggatgaataa atctgaagat atagcacaag catttcatgt tatccattcg
     1261 tttgataaga atatttctaa agagttaagt attgagaaaa tgcacgaaat agctgtcgaa
     1321 tttgcagaaa aagcttttcc aaattcgcaa attattgttg caagtcacaa tgataaagat
     1381 cattttcata gtcatcttgt tattaataat attgatatgg agacctgtaa gaggttgcgt
     1441 attgacccta aagatttaga acgaatgcga aaaattaacg atgaggtgct tgttgaaaat
     1501 ggactcgaac ctgtagacaa tcagtactat gaaaatttag aaaagagaac gaatctctat
     1561 tcaaacgatc caagtaaact gaaaaatgga aaaattaatt atcaagagtt tgtacaaaat
     1621 gcgattgaag ctacgttgga gaattgtgaa gtaaattcat ttgtgcgttt ttcggatcat
     1681 ttagcaaaag aatacaacat cgaagtttat caattttcaa aaacaaataa caaacttggc
     1741 tatgttcttt acaaacaaga tgtaaatttc tatgagcgta gagatgagct aattacttta
     1801 ggaagagaaa aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca
     1861 agaaagttag ggaaaaaata ttcatttgaa tctgtttttg aacgacttga aaagaattta
     1921 gaagaaacac atatgaataa ttttgttgat ctagatacgg aacaaattat agcgttagct
     1981 agtggagttg attataaaac tggggagtta aatgtaccgt ttgtggaatt agattccatt
     2041 gcacaaaagc agtttagtga tcatgagggt gctaaaaaaa tcattaaata tgatgatttc
     2101 ttgcgagaag tagacaggca ttatgcaaac acaatatctt tagaagatga tcaagaatac
     2161 acgattaaac ttcatactga tgtgaatcct agaactttac gtagtactaa aagtaaaaca
     2221 attttagtta aaaagacaga tcagaaagaa ttatttctac cagctgaggg ctttaaaaat
     2281 tgttctcata caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac
     2341 gatgtgaaaa tgtatcgtgc taaaaaagat tacgagttca cgcaacaaat gattgaaaat
     2401 cagcaaagga gacaacgaga ttttgagatg gatttctaaa ggaattagcc agtagacttt
     2461 ccagtttccc atcgatacgg agggaattgg aaagaggcgg ttttgagggc atacgccagc
     2521 tacccatcta cttgccagtc aatccgccct tgcttacgca gggctacttt ctggcaagtt
     2581 agatggggaa aacctctgag ggagcaagct ccctacagac tccctaaata aaaaaagata
     2641 aggagtaaaa acacatgtct gatccgtata ttaaagtgta ttgttcaaaa gaaactaaaa
     2701 caatttttga cagtttttac gaacagaaca aagggatttt cggaacaaaa gccaatatgt
     2761 ttcgtgtgat ggtatcaaat ttaccagtgt tagctaacca atcaaatgta aaattaagta
     2821 atcctgaaag caaaggaatt gaacaaaaaa tttctgaaat agaatcaatt gtggtgggaa
     2881 agttggatga aattgatcaa aagttatcgt attatctaaa aaacaaatgc aaaacggagg
     2941 aggggaaaga tgtttaaagt gtattttccg tttcagtggc tctttaaagg aattctttta
     3001 ttgctactag gattgattct ttttggaagc tatctaacaa ttaaagccag atattttcca
     3061 gaagaaaaac cagtaaaaat tgtaactaaa gatttaaaaa tgggtgaaac ttatcaggca
     3121 gatggggtcg aaataacgtt taaaaaggtt gaattgaccg aaaaaagaaa tgaatttgcg
     3181 caacatgatc gtgtattaca agtgacctat acaatgaaaa atgatacaga aaatccgata
     3241 cgtccgcaac aatggtcgat tgaaaataaa
//
LOCUS       DACEMS010000173         3265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEMS010000173 DACEMS010000000
VERSION     DACEMS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.180"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..434)
                     /locus_tag="IW735_002577"
     CDS             complement(<1..434)
                     /locus_tag="IW735_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045538.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYP"
     gene            complement(435..1292)
                     /locus_tag="IW735_002578"
     CDS             complement(435..1292)
                     /locus_tag="IW735_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1045539.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(1578..1865)
                     /locus_tag="IW735_002579"
     CDS             complement(1578..1865)
                     /locus_tag="IW735_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1045540.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(1855..2184)
                     /locus_tag="IW735_002580"
     CDS             complement(1855..2184)
                     /locus_tag="IW735_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1045541.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2261..2533)
                     /locus_tag="IW735_002581"
     CDS             complement(2261..2533)
                     /locus_tag="IW735_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1045542.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(2533..2796)
                     /locus_tag="IW735_002582"
     CDS             complement(2533..2796)
                     /locus_tag="IW735_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1045543.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(2902..>3265)
                     /locus_tag="IW735_002583"
     CDS             complement(2902..>3265)
                     /locus_tag="IW735_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045544.1"
                     /translation="FIPEDFQNLQLENMRLEDLFRLADDDKYRFEVLKIKKNELLNKL
                     YPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYVEDEVI
                     IKYMKPVIKLVNKNKGNG"
BASE COUNT          964 a          648 c          418 g         1235 t
ORIGIN      
        1 ggatattttt ctctgaacaa gtctagttct ttcaccacgt cattaaacaa atcttgctta
       61 tcttcttgcg taagattctg tttaatatac tcatctaaag cttttctaag caattcatct
      121 cttgtcatat tagtctcata cttttctact aaaactctgg aagtcgcatt taacaaagtc
      181 gacagtttta aaggatgctt ggtggaataa accttatcaa actttctgat tggtgcgcca
      241 actttttttg ttcttcctct actctgtctt tcaacattgg aggaagtttt tatttcttga
      301 acttggtgca cttctaattg cttattttca gatggtgtaa ttgcttgctg ctttttcttt
      361 gcttctgctt ctttttttat tttggcgagt cctttgttgt tcccaaaaat atcaagtcct
      421 ttgatgtcag ccaattactc cacctccgca taccaattct cgtgttcttc caattctttc
      481 aaaatactga taaataattc gtggcttttg cgatcccatt gttttaagag acctttcttt
      541 gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt tttgagtaca
      601 acatttttaa tcaaatgttc cccataaatc tcttgggctt gtttatacat ttcctgatct
      661 actgcgtcac cgctttccaa catgaaagga ataacaccag caacatataa atttgtatca
      721 aattctttca caaaaaagtc catatactca agatattgca tcgcatttct catcgatttt
      781 acttgggtct gtaagacgac gattgcccag tccgcaatgt acatcgcgga tttactatat
      841 acagaaatag aaggtgggac atctaaatag atgacgtcaa actcatcaaa taatggttct
      901 agcttttctt tcatatattt gaattgggat aatgaatcat tatattggtc tttgatccaa
      961 tcatcataca tttcaaagga catatctgct ggaagtatat agaggttatt gattacttta
     1021 tagacagcct ctttaggtgg aagattgttt atcaacacgt cagtcacagt attttcgtaa
     1081 acaatcggtt caccttctcc gcctccttct tcgttagtga agttcatatg cgttaagttc
     1141 atcacatcag aagtatttcc ttgcatatcc aagtcaatta tcaaggttct ttttccttca
     1201 ttaactgctg attcaaaccc taacatttgc gcgcacgtac ttttacctac gccaccttta
     1261 aaattaccaa ttacatattt cttcgctacc acgttcctac ctcttttctt ttagcaatat
     1321 tcatttgata ctattataac atatttattt ttagttacta ccaaaaaaaa gatatatatg
     1381 tactacctta taattaataa gtaaaaggta gtacataaat aaggaaaagt tgaaatatat
     1441 tgatttatag ctttcctcaa agtaatgacg ttatttaatt tatcgagaag cagagaatac
     1501 tcgcgacttt agtcatgtag acgaatcaga ttttcgataa aaaagaagcc gtccttctta
     1561 agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt ttcatgcgtt
     1621 ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac tctatatgtt
     1681 gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt tttttgttca
     1741 cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt ttctttcaat
     1801 ggcttctctt ttagcttttt taaatactta atgacatgtt ttgaaggaac gatttcataa
     1861 gccaatttct gttgccaact cctctttagt cattacttta gaattagtaa gtgtatcttc
     1921 agcaatatct ctaaaggaag aatgcatttt gttttttcta ttcttaaatt ctacaagaat
     1981 ctcatcttta tcgtagcctt ctgcaattat atcagacaaa atatcttcgc taaaatcata
     2041 aaattctttt tgtggttcaa caaattcata aagaatacca ttctctacca actttgcttc
     2101 aacttcgact ccattaggaa cattaaaatc tttaggtact gtcaacatga ttgaattgcc
     2161 ttgttttctt gtttttgtaa tcatttaaat ccctcatttc taacagtatg taattactta
     2221 gtaaatacat tataccacga gataatgtat ttacaagtaa ttactgtgac tcgtaatgcg
     2281 tccttgctga ataaatttca accacgtgtt catcttcatc taccttataa acaacccggt
     2341 gttgtctatt caatcttcta gaatacctac cttcatatgt aggttttaac ttctcaaatg
     2401 attgggtagg gagataggga tcttccttta aagtctgaat aaccttctca aattgaggtt
     2461 tcaaattaga ctgtttgatt ttctttaaat cagctttagc cgagttttta atcatcacag
     2521 aatagttatt cattataagt tatcccaatc aatttcatca acattagtaa atccactatc
     2581 atctttttct cgcttacgaa caacacccaa agtgcctatt tgctcaagta atagcgtttc
     2641 tttgattgct ttcaaatcat ccaaaccaat aagcaccgca ttattttcac tgcgctcact
     2701 aatgatttca atttccgtat gattctcatt tacatctttc aacaacttat aaaaattttt
     2761 ccgtgctgag ctaggattta agactgtttt tgccatatct actccccctc taatttatat
     2821 ctccattcta acgtacttaa ttaagtacgt aaattcgatt gttttgttta aattggagta
     2881 ccaaaacagc attgtaaagt gttatccgtt tcctttattt ttattaacaa gttttatcac
     2941 aggcttcata tatttgatta taacttcatc ttctacgtag atatctgtta tcattacgac
     3001 atctgtacta tttcgattga gtactttcac ttgatctcca gcattgattt tccctctaaa
     3061 tcttttagga atgaaccaac tatacatatg attagcatca ccaaaatgtg cttttatcgc
     3121 attctcagga tataacttat tcaatagttc attcttttta attttcaaaa cttcaaatct
     3181 atacttatca tcatctgcta atctaaataa atcttctaaa cgcatgtttt ctaactgtaa
     3241 gttttgaaaa tcttccggta taaat
//
LOCUS       DACEMS010000174         3231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEMS010000174 DACEMS010000000
VERSION     DACEMS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.181"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            183..1137
                     /locus_tag="IW735_002584"
                     /pseudo
     CDS             183..1137
                     /locus_tag="IW735_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1154..2178
                     /locus_tag="IW735_002585"
                     /pseudo
     CDS             1154..2178
                     /locus_tag="IW735_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2184..2663
                     /locus_tag="IW735_002586"
     CDS             2184..2663
                     /locus_tag="IW735_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1045545.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2679..>3231
                     /locus_tag="IW735_002587"
     CDS             2679..>3231
                     /locus_tag="IW735_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1045546.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT          979 a          539 c          751 g          962 t
ORIGIN      
        1 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
       61 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
      121 tggacggtat tactgatata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
      181 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
      241 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttgggctttg
      301 atggactgaa agtagatgac aagagctgga cagtgtggaa ttggccacaa ggtgtcggtc
      361 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
      421 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
      481 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
      541 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
      601 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
      661 tgacagtgct tcctttggca aaaatcggga aactgctggt tgacgattgc tattccagag
      721 atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt gatcgaaaca
      781 cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt atggcatacg
      841 ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt tgcttacggt
      901 attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt cgcttatcgt
      961 gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa cgtttcaatc
     1021 gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac agcaatgcct
     1081 tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta ttgttaaaag
     1141 aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc ttagcctagg
     1201 tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg cagatgcaga
     1261 cgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagt aattgatgca
     1321 atgaatgaat ttgggcgttt agtaaaagaa aaaacagatg gttccgtaca gatccagtat
     1381 ttcccagatg gacaactggg tggagaacgt gaactgatcg aattgacaca gtcaggagca
     1441 gtagatttca caaaagtaag tgcctcagcg ttggaaagtt tctctaaaga ttattcgatt
     1501 ttttccgttc cttatctatt caatgatgaa gaacatttct ttaaggtgat ggataatgaa
     1561 gaaataatgg agccaatcta ccaatcgacc aaaaatttag gatttaccgg attgacctat
     1621 tatgattctg gtcagctcag cttttatatg gtagacggtc cgatccatac gccggaagat
     1681 ttgaagggaa agaagatccg tgtgatgcaa agcgaaaccg ctatcaaaat ggtagagctt
     1741 ttaggcggtt cgcccgtacc tatgggaagt gatgaagtgt acacgtctct tcaatccaat
     1801 ttgatcaacg gatcagaaaa caatgaattt gtcttgtata cagctggaca tggcggggta
     1861 gctaaatact attcctatga tgagcataca cgtgtgccag atatcatcat catgaatgat
     1921 gccatcaaag aacagctaac tgatgaacag gaaaaagcca ttgaagaagc agcgaaagaa
     1981 tcaacggttt ttgaaattga agcttttgca aaagcaattg aagaagaaaa agctttagca
     2041 caagaagaac atggagtcca attcaatgat gtagataata caccgtttcg agaggcagtt
     2101 gccccacttc atgatatatt caaaaacgat cctacctaca aagaccttta tcaaaagatc
     2161 caagagcaag gagcgtagct gtaatgaaaa tgattttaaa caaggtatta gagatcctgg
     2221 gagctgtttt gcttgttggg atggtcgctg ttgttttatg gcaagtattt tccagaacgg
     2281 ttttaggaaa tccaaatacg gtcacggaag agcttgttcg attcggtctt gtctggtttg
     2341 ccatgttgtc ttccgcgtat gttgtgggac aaaaagcacg tttagccgta acggttttaa
     2401 gtgaaaaatt aactggcaaa aaaagtaaaa ttttagaagt tgttgttcaa agtttatttc
     2461 tggtatttgc tgcgatgatc atggtgtatg gcggatggaa tgctgtcaca ttgacaatgg
     2521 ggcagatctc gccttcttta agtattccta tgggatatgt ttatctgtca gtacctgtat
     2581 cgggggtact catcattatc tatagcctga tcaatctaat cgatacatta agcaacaagc
     2641 cagcaattca agaaaaaata taaaaatgga ggaataaaat ggcaatagaa gccggattag
     2701 tgttattttt tgtatttgtt gtgttgcttg tattagggat gccgattgct ataagtattg
     2761 cattctcatc gatgatgact ttattgttag ttataccgtt tgatgtttct gcttttagtt
     2821 cagctcaaaa aatggtggga agtatcaata gtttttcgct gatcgctatt ccgtttttcg
     2881 ttctctcggg cattatcatg aataatggag ggatagctaa gaaactagtg gattttgcga
     2941 tgttgttcgt cgggcgcgtg ccgggggctt tggctcatac gaatgtgtta gggaatgctt
     3001 tatttggttc catctctagc tcggccattg ccgcttctac cgcgattggt ggagtgttga
     3061 tccctcagca agtggaagaa ggatatgacc ggaagtttgc gacagcggtc aatatcgcgt
     3121 cagctccgac aggtatggtc attcctccaa gtactgcttt tatcatgtat tcacttgttg
     3181 ctggtggtgc ttcgatctct gctttgttta tgggaggtta tctcgtaggc g
//
LOCUS       DACEMS010000175         3190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEMS010000175 DACEMS010000000
VERSION     DACEMS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.182"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            210..476
                     /locus_tag="IW735_002588"
     CDS             210..476
                     /locus_tag="IW735_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1045547.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            503..802
                     /locus_tag="IW735_002589"
     CDS             503..802
                     /locus_tag="IW735_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ1045548.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            976..1527
                     /locus_tag="IW735_002590"
     CDS             976..1527
                     /locus_tag="IW735_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ1045549.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1673..1966
                     /locus_tag="IW735_002591"
     CDS             1673..1966
                     /locus_tag="IW735_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045550.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1959..2255
                     /locus_tag="IW735_002592"
     CDS             1959..2255
                     /locus_tag="IW735_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1045551.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2330..>3190
                     /locus_tag="IW735_002593"
     CDS             2330..>3190
                     /locus_tag="IW735_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1045552.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENG"
BASE COUNT         1198 a          439 c          635 g          918 t
ORIGIN      
        1 atttagaaaa acttactaaa caaagagata tgaaaaactt taataattgt gtagttttga
       61 taattataac tgaattaact ttatttacaa atatatatta tatccatgaa gcccaatctt
      121 ctttaataga agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata
      181 aaggataacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt
      241 tatgttggaa tctaatcgat caaaacgttc aaaagcaatt tcctttagac tatcttcaaa
      301 tttttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccgg
      361 aagaaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa
      421 tatgggccat tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactagtatc
      481 taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc
      541 acagctggtc actgcttctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga
      601 aattgaaatg ggtatgcttc ctgaagatgt tcatctacca cgtttagtga tgacacatct
      661 ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat
      721 taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca
      781 gattggagga acggatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag
      841 atatccttac cgcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt
      901 tcaactagta ttgacactta taacggcaat ggtaattgaa gtgcttgaac aaaaaatagc
      961 ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga
     1021 gatcctcatc atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa
     1081 gcggaagata aattagcagt agtcattact attcgtccaa cagatacaga accatttacg
     1141 aatgatttga gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt
     1201 attgctgtga atcttttttc gtacacaaaa gcgaaaagtc ctcgggtatt tcttcgagga
     1261 aatgatgaac aatctttgga aatattaacg actgttttta cagaaaagaa aatcaatacc
     1321 attatttttg cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcaaag
     1381 actatttacg atcaattgag tgcgaaacaa aagaagatga ccaaagtatt gataaatgca
     1441 gaaggtaagt tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat
     1501 agtcaattat tatttcaaga aaaataacat tttattttct catgttactt ataagtagtt
     1561 ttacatgctt tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg
     1621 tacccagtaa tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat
     1681 tgatgaaact tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga
     1741 actggattca gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt
     1801 tccaatgaaa atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca
     1861 ggaaaaaact gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat
     1921 tcaaaaagta attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag
     1981 gtggtttaat ttacggtgga tattggttat ataaatatat gcttgcgcat ccgaatatag
     2041 ataaaggaaa agtaattaag acagttgggg taatcatagt tggactcata gctctaacta
     2101 agaaaaataa tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa
     2161 gggaaatttc tactagtttt aatccatctg agagtcctca aggaagagac cgctgccata
     2221 cgggctatcg acattattgg gaaagaatcg attaaaagat taccatttag atcaattaat
     2281 tgtctgatag aataagtacg tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg
     2341 gctgttatta ctaataggaa tgggaatttt attcactggt tgtacatctg agaatacgag
     2401 gactaaagcg actatagaaa ctgatacaac aattgagact aaaaaaagta aaacgcaatc
     2461 aactgaacaa actacaacag aaatgaagca atctgtggaa acaagtgcaa gttcaatcac
     2521 tgaatcttct tctgaagagg ttaagaatac tttgtgggat acgaataaag cttccaaatt
     2581 agaaactttt gtcacacaat ggggaaagac attaggtcaa gaatataaaa gctacaatct
     2641 tcagaataat gtaagtctgt atggcactcc tttaccacaa gccgtgatta atggcgattg
     2701 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2761 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2821 gcatgtttac tttttcggtt ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2881 tcaaggtaat gaaaataatt atctgtactt taacgaaact caaaatcaac agttaaaaca
     2941 aggtttcagt gatattgtga atggacaaac tcctcaaaca ccagtagtag aagaatctac
     3001 tcaacaagca acaactacaa cgacacaatc tacaagtagt tcaggaattt caagttggga
     3061 agctgctaaa gaatctgtgc taaataataa agctttatgg ttgtatgatt ctcctgataa
     3121 aaatgtatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3181 tgaaaatgga
//
LOCUS       DACEMS010000176         3167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEMS010000176 DACEMS010000000
VERSION     DACEMS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.183"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(862..1203)
                     /locus_tag="IW735_002594"
     CDS             complement(862..1203)
                     /locus_tag="IW735_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1045553.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="IW735_002595"
     CDS             complement(1203..1433)
                     /locus_tag="IW735_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1045554.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="IW735_002596"
     CDS             1720..2058
                     /locus_tag="IW735_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045555.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="IW735_002597"
     CDS             complement(2263..2502)
                     /locus_tag="IW735_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1045556.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="IW735_002598"
     CDS             complement(2478..2825)
                     /locus_tag="IW735_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1045557.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1004 a          579 c          489 g         1095 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattccgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgac
//
LOCUS       DACEMS010000177         3152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEMS010000177 DACEMS010000000
VERSION     DACEMS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.184"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..2545)
                     /locus_tag="IW735_002599"
     CDS             complement(53..2545)
                     /locus_tag="IW735_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1045558.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2552..3019)
                     /locus_tag="IW735_002600"
     CDS             complement(2552..3019)
                     /locus_tag="IW735_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1045559.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          835 a          694 c          517 g         1106 t
ORIGIN      
        1 cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt aactataggg
       61 aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct tttgaagcac
      121 ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg ctcaatcgat
      181 cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca ggatcaaatc
      241 cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata tcttgttctt
      301 tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt tcatctttac
      361 tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt cggaaagctt
      421 tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta acatcttgga
      481 cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa
      541 tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa tgaccatcat
      601 ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact tcatctaaga
      661 gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct tcatcatacc
      721 ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc ataaattctg
      781 acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa agtgctttgg
      841 ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca attggacgat
      901 caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct
      961 catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag cgttcgcttt
     1021 cttttttctc caactgctgt agagggacac cagtccactc cgatacaact gtagcaacat
     1081 cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt gcagaaactt
     1141 tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt
     1201 cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct agtttgatca
     1261 cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct gattcatcga
     1321 tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca gaaagattga
     1381 ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca tatcttgtac
     1441 gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact tgaatgcgag
     1501 caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca tctagggtcg
     1561 ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata ttcgaagcat
     1621 caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca atgaataaga
     1681 ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct tcaaattcac
     1741 cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg cgcttttctt
     1801 gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca gcaatggctg
     1861 ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt cggctaagaa
     1921 tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc ttgttttctt
     1981 ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt
     2041 gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca gcagaatcac
     2101 cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg atacctaagt
     2161 tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc aaaatatgtt
     2221 ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa gcaaaaactt
     2281 gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg ataccaccat
     2341 aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc atttcacgta
     2401 atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca tgttctgaac
     2461 taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat actgcttggg
     2521 ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa cttaaacgtt
     2581 ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg aaattcaatg
     2641 tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt atttggtctt
     2701 cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca tcaaataact
     2761 gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca attcgtatat
     2821 aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta aaacgggtat
     2881 taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt tcaatccttc
     2941 gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca attaaatccg
     3001 aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct taattataac
     3061 gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt tagaatcaac
     3121 aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DACEMS010000178         3140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEMS010000178 DACEMS010000000
VERSION     DACEMS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.185"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..736
                     /locus_tag="IW735_002601"
     CDS             <1..736
                     /locus_tag="IW735_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1045560.1"
                     /translation="LVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENS
                     QKNLIVQNLNYGTNQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDI
                     KESHNDDLFESLSTKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDN
                     WYKKFNYAIKSAEKEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIK
                     QKNNYIFTSLKNKALELIKLQKKEGI"
     gene            738..956
                     /locus_tag="IW735_002602"
     CDS             738..956
                     /locus_tag="IW735_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045561.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            943..>3140
                     /locus_tag="IW735_002603"
     CDS             943..>3140
                     /locus_tag="IW735_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045562.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGT"
BASE COUNT         1171 a          438 c          623 g          908 t
ORIGIN      
        1 tcttgtgaaa agaaaaaatg gcttcaataa aaataaaatg caaatgttcc caaattttta
       61 ttatttgtta aagcctgtac tgtgcaaaga tgatgtttat aaaattaata agataaccca
      121 tcaagaaaat agtcaaaaaa acttgatagt tcaaaatttg aactatggaa caaatcaata
      181 ttctgttgat agttcaaaat ttgaactatg cgacacatca atgaaaacaa gagacaaaaa
      241 caattttgct gatagttcaa aaaatgaact agaattatat aagtcttata aagatagtaa
      301 agatattaaa gaatcacata atgatgatct ttttgaatca ctttcaacta aaaataataa
      361 attagatgaa gaattgatcg aaacatttat tgaagaaaat cttcttaaac aaatgtgggg
      421 agaaagtatt gtgaattgct taaaactggc tagcaatagt gattatcgtc aattcgacaa
      481 ctggtacaaa aaattcaatt atgcaattaa atctgctgaa aaagaacaaa aagttcaact
      541 gaaaattatc tatgaaattt gtaataatag ttattttgta gatcatgtta gagaacagct
      601 agcaatgact ttacgtgact tgatacgaag agcaaaaaca gatcatcgta tcaaacaaaa
      661 aaataattat atctttactt ctctaaaaaa caaagcgtta gaactaatca agctacaaaa
      721 aaaagaggga atttaaaatg attgaaaatc atctagtaga taagattcac tgtcttgatc
      781 aaaacgcaga tactcttgag ttaaccgaga gtatctgctc aaatactatt gttctcgatt
      841 taaaagctgt taatgatgat tacgtaactc atatcataat tgacagaaaa atagctcaga
      901 aactctctga gagcctaaga aaatgggctg agggaggtaa caatgaaaat aattaaaaaa
      961 aagattgcta atagaaaaac aaattggtta ccggttattt ttcttgttct aatagctgtg
     1021 attcttttta cgagtaacat tgcagaaggc agtattttca attggtttga tgatactgag
     1081 agtcaaactg agtttttaac taatccgctt taccagaatt atttgaaaaa atacaacgga
     1141 aatttttaca gccagttcgg tatgtggata ggctggtctg ttataaaatc aatgttcgct
     1201 gttgtcgatg gcattcaaaa tatgattccc gaagttttag atttgtttag ctttgtagaa
     1261 agtaccggat tgaacaaagt ctatcaagct gttttaagta ccattgttgt tggcttaatg
     1321 atactttcac tagtgattat tggttataaa atgattacag gtaagggaac ggttgatcta
     1381 aaatctgttg gaatgaatgt tgtaatgtca atttcattga ttcttttaat gccaacgttg
     1441 attcacagcg gaatagagtt ttctaaaaca ttttataatg atacgactac gattacaaat
     1501 tcggacgaag gcgtggcgtg gtcgctgatt gaacaagggg ttactgattt agcttatatc
     1561 aataaaacag atcaatattc catgatcgat aaaacgaatg accggaacaa tctaacaaaa
     1621 gaaaattttt ggaaaacgga tcttacgcaa gtattgacag ataatgtact cgacaaaatg
     1681 gaaaaagaga acccaatagc tgataatctg cgttatgaat tagttgaaaa ttctgataat
     1741 gaatttgttg ctaccaaatt ttcggataat tttctgtcga cctttgttga gggcattaaa
     1801 tcaggatatt atcgctatca agcaaatcta tgggggattt cttttggatt gattgcttta
     1861 gcggtagcgt atgtttttag tgctttcatt ataattacag caatactcga gctagcgttt
     1921 aaacgcgtac ttggagtttt agtatttgct actgacttgg aaacaggcca acgatcaaag
     1981 caagttttgt cagatatttt acagtgttat ttgacagtcg gatttcaagg gttcggattg
     2041 tctatgtttg caatgtttgt taattatctc ggaacaggta gcggttcttc tgtaaatcct
     2101 tttataaaag cagtagcgta cgtctgcgct gttattgtgc tgataaaggg tagtggaaca
     2161 gtcatgcgtt actttggcgt ggatattggt ttaaaagaag gctatggcca gcttgcttca
     2221 gcatttggca tgggagctat gctgtttcgt aaaggaacag gtggtatgaa ccgatctaaa
     2281 ggtgggaaaa atagtggtgg aaacgatcaa gcggaagggg aagaacgaaa acctgaacgc
     2341 aactttggtc aatcactttc tcaaaaagct aataaaacgg ggaaaacact tgggtatgct
     2401 cgtgaacgag gattatcagg cttagcttcg gatggagcta caatggccag tgaaagagca
     2461 acaaaaccat ttaagagtat gcgtgatatg gctaatgata ccaagaataa attcaaagaa
     2521 ggtttagatg atggtaccgt gtacgctatt aataaaaata gcaaacctat agtagctaaa
     2581 aacaaagaag atgaaactgg aaaatatgct gattctatgc caacacgtct ttcggataga
     2641 aaagatggcg ctaaagttga aaacgctgat agaattatgt ctagttctga acgtatgcgt
     2701 gaagcaatga aaaataatgc tgaatcaaat attaatcctg caagtgcaat tcagcaaaaa
     2761 gttaaacaag atattgaaga acgtaaaaat gctatgcacg gtcaagctaa gtctgctgaa
     2821 gtattaatta atcagaagcg acaggaagca aaatacacgc ctgaagcaat gaatcgtgag
     2881 gaaatgttaa ggaaacgtgt agaagggcgt acaggcgcta atatggacga aaaagaagcg
     2941 ttgactaaag aaagaatcca agaagctaaa aattcaaata ccggattaga aaaatcagtc
     3001 aaagaaaatc tacaaaattc tacaagtagt caagataggc gctcagtaga tgttcgagaa
     3061 aacttacaaa atactacaag tagtcaaaat ggaaatcgaa cagtatatgt acgagaaaat
     3121 gtacaaagta gtgggacaag
//
LOCUS       DACEMS010000179         3014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEMS010000179 DACEMS010000000
VERSION     DACEMS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.186"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..295)
                     /locus_tag="IW735_002604"
                     /pseudo
     CDS             complement(1..295)
                     /locus_tag="IW735_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(369..650)
                     /locus_tag="IW735_002605"
                     /pseudo
     CDS             complement(369..650)
                     /locus_tag="IW735_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1112..2494
                     /locus_tag="IW735_002606"
                     /pseudo
     CDS             1112..2494
                     /locus_tag="IW735_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2730..>3014)
                     /locus_tag="IW735_002607"
     CDS             complement(2730..>3014)
                     /locus_tag="IW735_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1045563.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT         1050 a          547 c          506 g          911 t
ORIGIN      
        1 ttaagccgtc cgcatcaatt gcacgataaa gataatgcca acgtccctta attttgatat
       61 aggtttcgtc cattttccat gaatagaagg attgtctatt tttcttcttc caaagataat
      121 agaggacttt gctgtactct tatacccaac gataaatcgt agtatgacaa acatttattc
      181 cacgatcata taacaattcc tgaacttcac gatagcttag attgtaacgc aggtagtaac
      241 caacagcgac aataatgacg tcttttttga attgtttgcc tttaaaatga ttcattactc
      301 tgtcctctct gtcttttttc tcaattttac actaaaatag attttttgga aaactttgca
      361 acagaaccga aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt
      421 gatttactaa aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct
      481 tctttcggaa atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa
      541 ccttctctca atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta
      601 gccaataact caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc
      661 ttcctatctt gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa
      721 tttaattgta ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc
      781 cgactcctct tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt
      841 ctgaccataa ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc
      901 aaaaactttt aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt
      961 ctgaaagaaa aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat
     1021 tgacactcaa agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg
     1081 acgaaaaaat aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc
     1141 aattcagtta tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta
     1201 tccgtcgatt ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca
     1261 tcaggagcac cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca
     1321 ttattttgaa tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag
     1381 agagtccttg cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag
     1441 tgcggtaaag ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca
     1501 aactaatagg aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg
     1561 gaaaaacacg gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa
     1621 acaaaaagag ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca
     1681 gctcgaaaaa gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa
     1741 tcttctttgt ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt
     1801 caccttgaaa gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa
     1861 acaagcactt gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt
     1921 tcaatcataa cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg
     1981 gtaaaaagct ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta
     2041 aattttctag acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccta
     2101 atttattaaa tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta
     2161 ctgaatttag aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg
     2221 gtgctattgt tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa
     2281 gggttattca ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat
     2341 gttttatcaa cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga
     2401 ttatattcat tggtataata atcaacgaat taagttatct ttaggagggc taagccctct
     2461 tcaatatcgg aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc
     2521 cccttttttt tgtaaaaaac tattgcacaa tacaagtagc ctacggctat cgaaatttcc
     2581 ataactttcg agcaagaact tatctcattc aaggcttgat cttccaagta aaacaaaaac
     2641 cagttaagca ctctgcctaa ctggttatga ctaaattttc agttgtatct ttctctacca
     2701 acactaaatg tcgaagagcc ttttttcgtt tatgaatgta agaccgcttc tcgagccaat
     2761 gccaaacaac cgagcgttcc agcgttgtct tctaatgctg gggtcacgat atatttgctt
     2821 aactcaggtg tttcgacata cccattgacc aattttgcaa atgcgtcgtg gacgttttgc
     2881 agcatatggc gttgtttcat cacaccaccg ccaaaaatga tcacatctgg agaaaacatc
     2941 aaggttgtat tgtaggcaca ttgagcaata taggaggctt cgatttccca ataaggatca
     3001 tcttctgcaa cctc
//
LOCUS       DACEMS010000180         3008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEMS010000180 DACEMS010000000
VERSION     DACEMS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.187"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            441..725
                     /locus_tag="IW735_002608"
     CDS             441..725
                     /locus_tag="IW735_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045564.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            728..1144
                     /locus_tag="IW735_002609"
     CDS             728..1144
                     /locus_tag="IW735_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045565.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1281..2000)
                     /locus_tag="IW735_002610"
     CDS             complement(1281..2000)
                     /locus_tag="IW735_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1045566.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2653..2748
                     /locus_tag="IW735_002611"
     CDS             2653..2748
                     /locus_tag="IW735_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1045567.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT         1008 a          565 c          478 g          957 t
ORIGIN      
        1 aaaaaaactc ttagaagcga ttttagagcc atttaaaccc acttaattat attctccatg
       61 aaattcgctc catgcgtcaa aagctgcgtc tgaaattccg ccgtcttcgt tcactagttg
      121 cattaatttt tccaacgatc gttttctttc gggtgtcata tttttatatt ccaacaattt
      181 ttcgtctgtt aaatttggtt atcgattaca cgtgcagcac tcccccccga attttaaaga
      241 gcataaggaa acgtgtaccc actttctaaa aaaagtgggt agataggctg tgcctatcac
      301 gtttctcaga aaaaaatttg atatcacttt ttcgccatcg tgggtagctt atgtatagca
      361 ttttggcaaa aaacagctat acaaatatag tatttgtatg gtatacataa attatacata
      421 attgcaaaag gagatttttt atggacattt tagttcggaa tattgatcca aagtttgttg
      481 caacaataga caaacgttgc caagaactca cgaataaaac aggagaaaaa tggagtcgaa
      541 ataaatatct aaaagtttta atcgaaagtg attttgaccg tcaacttacg acttacaaaa
      601 aagaccaatt cgatttattg ctcgataaat ttattgctat ccaaacagaa aatactcaaa
      661 tattaagcga gtatgtctac acaaataatc aaatcattga aatgcttatc ggaggaatga
      721 tataaccatg agaaaaactt ctgtccaaaa acatgttact ttgtacccta gtcatgttga
      781 cgttttagat tccatcatcg aaaaaaatcc agatatcaat aactactcgg aagccattcg
      841 ctttttatgt atgagttatg aacccgatac caacacgacc actaccagca atcaggcaaa
      901 actaaatgct atgggaaaag aaatttccat catgactgaa atcatcgcag aatttgctga
      961 tttatatcta aaagatacag gcgttcttat tggcacaaat tctaaagtat atcaagaagt
     1021 gaaagaaaaa atcgaagcta aactaaatcc atcaaatcga aatcgaccaa agaatcatca
     1081 aaaagaaacc agtcaaacag tcacttctag aaatttcgac ccaacaaatt ttctaaaacg
     1141 ataaaaaaca gataggagtt attcctatct gttttttatt tagaagcccc atacaatatg
     1201 ctagaatgta ctagcaatct tagtcggttg taagggactt gtctacttgc ttaatgtaag
     1261 gggggcgagt tcttttttat ttattcttct aaccagttac gcagagaaat ctttggcaaa
     1321 tctgacgtta ggcttgaaaa agtaaattct aatttggcga ttttattgcc cttttttgct
     1381 tttatttttg tgactttaag atcattgaag tattttttta gttctcgtaa tgcaggattt
     1441 aatacttttt gatcaatgtt acccatttga tagtattctg ggatatccat taagctccta
     1501 aactcttcaa tacgaactac ataataacca gtagaacggt actgcataag cagtcggaat
     1561 agagtttttg catacgtaga acgcaacgac gtgaaagcag ataattcaaa tctactaaat
     1621 tcagctgtta aaccattaat aatatattct aggtccggat taacactgac ttcaacaaac
     1681 ttttttgttt tattgatttc aaaaccagta aacaaaacaa agtagcgaat agaatcctca
     1741 tcttcagtac ggtaagtaag attaagcata tctttataga gcttttctaa atcaccgaca
     1801 aaggcttttg tagcagtcat cttataatca cttaactctt ttaattcctc gaaagaaaat
     1861 ctaacttttt tcaaaccacg atctttcata cgagcgcata atgcaaaaaa taaatccatc
     1921 tgcttagcat tgaaattttt cagcggtact aaattcaatt cattttgata tttgactgtt
     1981 aattccgttt tttcatccat tcctttcttc cccccttaca aataaataat aacctttaac
     2041 aaccaactcg taaataggtt ggttgttatt atacaaaagg atattagtac tcagataaaa
     2101 agttgttagt actcagataa aaagttgtta gtactcagat aaaaagttgt tagtactcag
     2161 ataaaaagtt gttagtaccc ttctgtatcc gttgggagag taaggctcaa ctgcccccta
     2221 aaaagggttc taaaaaatct ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa
     2281 tttcccgcca aaaaatcacc cactttttaa aataaacagg ttcgcgcatg atattaaact
     2341 gccaatctag ctccctatgg tcgctgtaag aaggagtgat cgctacgctc tcacatttta
     2401 cttgcagcag tcgcctttag gctccttcgc caaaaataat tcattaggga ctcctttgtg
     2461 gcgtcctgag agctcgtcta agccgttcta agacaagatt cgataaaaac tatgtccttc
     2521 gttctaaagc gccttacagg cggtcagggc aagccctaac aaccccaagg caataaaaaa
     2581 cttaggctta tttttgcttt tttattgtat aattgattgt cgcctatccg acagtcttgc
     2641 gactggaagg aggtgtgttt cgtgttcact aactttattt gctcccttct tgttgggctt
     2701 ttaagcgctt tgttcagtta ctggttgaat aaccgaaaca agaagtaaaa cttaattaag
     2761 gcgactcagc ccacaaaaaa agccccagag aatccgacct ctctggggat ttcgtgtgtt
     2821 tcgtgttcac ttatttgcaa tcaaattata acagaaataa atatttattt caaaaatatc
     2881 ctacaaaagt ctaaaatccg taattatttc ttatatgcaa catttacaaa taaagtcgct
     2941 ctgagagctc gtatttttcg atttaagacg ttttatttag tttttggtgt atttcattgg
     3001 tccaagct
//
LOCUS       DACEMS010000181         2992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEMS010000181 DACEMS010000000
VERSION     DACEMS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.188"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(106..1053)
                     /locus_tag="IW735_002612"
     CDS             complement(106..1053)
                     /locus_tag="IW735_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1045568.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IW735_002613"
     CDS             1299..1763
                     /locus_tag="IW735_002613"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1045569.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IW735_002614"
     CDS             complement(1825..2868)
                     /locus_tag="IW735_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1045570.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          990 a          593 c          571 g          838 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DACEMS010000182         2916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEMS010000182 DACEMS010000000
VERSION     DACEMS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.189"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            203..1171
                     /locus_tag="IW735_002615"
     CDS             203..1171
                     /locus_tag="IW735_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1045571.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1164..2195
                     /gene="vanA"
                     /locus_tag="IW735_002616"
     CDS             1164..2195
                     /gene="vanA"
                     /locus_tag="IW735_002616"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1045572.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2201..2809
                     /locus_tag="IW735_002617"
     CDS             2201..2809
                     /locus_tag="IW735_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1045573.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          862 a          559 c          708 g          787 t
ORIGIN      
        1 attttttagg aaaatctcaa ggttatcttt actttttctt aggaaattaa caatttaata
       61 ttaagaaacg gctcgttctt acacggtaga cttaataccg taagaacgag ccgttttcgt
      121 tcttcagaga aagatttgac aagattacca ttggcatccc cgttttattt ggtgcctttc
      181 acagaaaggg ttggtcttaa ttatgaataa catcggcatt actgtttatg gatgtgagca
      241 ggatgaggca gatgcattcc atgctctttc gcctcgcttt ggcgttatgg caacgataat
      301 taacgccaac gtgtcggaat ccaacgccaa atccgcgcct ttcaatcaat gtatcagtgt
      361 gggacataaa tcagagattt ccgcctctat tcttcttgcg ctgaagagag ccggtgtgaa
      421 atatatttct acccgaagca tcggctgcaa tcatatagat acaactgctg ctaagagaat
      481 gggcatcact gtcgacaatg tggcgtactc gccggatagc gttgccgatt atactatgat
      541 gctaattctt atggcagtac gcaacgtaaa atcgattgtg cgctctgtgg aaaaacatga
      601 tttcaggttg gacagcgacc gtggcaaggt actcagcgac atgacagttg gtgtggtggg
      661 aacgggccag ataggcaaag cggttattga gcggctgcga ggatttggat gtaaagtgtt
      721 ggcttatagt cgcagccgaa gtatagaggt aaactatgta ccgtttgatg agttgctgca
      781 aaatagcgat atcgttacgc ttcatgtgcc gctcaatacg gatacgcact atattatcag
      841 ccacgaacaa atacagagaa tgaagcaagg agcatttctt atcaatactg ggcgcggtcc
      901 acttgtagat acctatgagt tggttaaagc attagaaaac gggaaactgg gcggtgccgc
      961 attggatgta ttggaaggag aggaagagtt tttctactct gattgcaccc aaaaaccaat
     1021 tgataatcaa tttttactta aacttcaaag aatgcctaac gtgataatca caccgcatac
     1081 ggcctattat accgagcaag cgttgcgtga taccgttgaa aaaaccatta aaaactgttt
     1141 ggattttgaa aggagacagg agcatgaata gaataaaagt tgcaatactg tttgggggtt
     1201 gctcagagga gcatgacgta tcggtaaaat ctgcaataga gatagccgct aacattaata
     1261 aagaaaaata cgagccgtta tacattggaa ttacgaaatc tggtgtatgg aaaatgtgcg
     1321 aaaaaccttg cgcggaatgg gaaaacgaca attgctattc agctgtactc tcgccggata
     1381 aaaaaatgca cggattactt gttaaaaaga accatgaata tgaaatcaac catgttgatg
     1441 tagcattttc agctttgcat ggcaagtcag gtgaagatgg atccatacaa ggtctgtttg
     1501 aattgtccgg tatccctttt gtaggctgcg atattcaaag ctcagcaatt tgtatggaca
     1561 aatcgttgac atacatcgtt gcgaaaaatg ctgggatagc tactcccgcc ttttgggtta
     1621 ttaataaaga tgataggccg gtggcagcta cgtttaccta tcctgttttt gttaagccgg
     1681 cgcgttcagg ctcatccttc ggtgtgaaaa aagtcaatag cgcggacgaa ttggactacg
     1741 caattgaatc ggcaagacaa tatgacagca aaatcttaat tgagcaggct gtttcgggct
     1801 gtgaggtcgg ttgtgcggta ttgggaaaca gtgccgcgtt agctgttggc gaggtggacc
     1861 aaatcaggct gcagtacgga atctttcgta ttcatcagga agtcgagccg gaaaaaggct
     1921 ctgaaaacgc agttataacc gttcccgcag acctttcagc agaggagcga ggacggatac
     1981 aggaaacggc aaaaaaaata tataaagcgc tcggctgtag aggtctagcc cgtgtggata
     2041 tgtttttaca agataacggc cgcattgtac tgaacgaagt caatactctg cccggtttca
     2101 cgtcatacag tcgttatccc cgtatgatgg ccgctgcagg tattgcactt cccgaactga
     2161 ttgaccgctt gatcgtatta gcgttaaagg ggtgataagc atggaaatag gatttacttt
     2221 tttagatgaa atagtacacg gtgttcgttg ggacgctaaa tatgccactt gggataattt
     2281 caccggaaaa ccggttgacg gttatgaagt aaatcgcatt gtagggacat acgagttggc
     2341 tgaatcgctt ttgaaggcaa aagaactggc tgctacccaa gggtacggat tgcttctatg
     2401 ggacggttac cgtcctaatc gtgctgtaaa ctgttttatg caatgggctg cacagccgga
     2461 aaataacctg acaaaggaaa gttattatcc caatattgac cgaactgaga tgatttcaaa
     2521 aggatacgtg gcttcaaaat caagccatag ccgcggcagt gccattgatc ttacgcttta
     2581 tcgattagac acgggtgagc ttgtaccaat ggggagccga tttgatttta tggatgaacg
     2641 ctctcatcat gcggcaaatg gaatatcatg caatgaagcg caaaatcgca gacgtttgcg
     2701 ctccatcatg gaaaacagtg ggtttgaagc atatagcctc gaatggtggc actatgtatt
     2761 aagagacgaa ccatacccca atagctattt tgatttcccc gttaaataaa cttttaaccg
     2821 ttgcacggac aaactatata agctaactct ttcggcagga aacccgacgt atgtaactgg
     2881 ttcttaggga atttatatat agtagatagt attgaa
//
LOCUS       DACEMS010000183         2778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEMS010000183 DACEMS010000000
VERSION     DACEMS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.191"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..197
                     /locus_tag="IW735_002618"
     CDS             <1..197
                     /locus_tag="IW735_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045574.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IW735_002619"
     CDS             314..811
                     /locus_tag="IW735_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1045575.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IW735_002620"
     CDS             898..1290
                     /locus_tag="IW735_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1045576.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IW735_002621"
     CDS             1274..>2778
                     /locus_tag="IW735_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1045577.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACEMS010000184         2739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEMS010000184 DACEMS010000000
VERSION     DACEMS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.192"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(60..404)
                     /locus_tag="IW735_002622"
     CDS             complement(60..404)
                     /locus_tag="IW735_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045578.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(480..1727)
                     /locus_tag="IW735_002623"
     CDS             complement(480..1727)
                     /locus_tag="IW735_002623"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1045579.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(1724..2530)
                     /gene="proB"
                     /locus_tag="IW735_002624"
     CDS             complement(1724..2530)
                     /gene="proB"
                     /locus_tag="IW735_002624"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1045580.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          773 a          570 c          522 g          874 t
ORIGIN      
        1 aaaagtccgg cagttttttg ccgatcttca aggcactcta cttatcgtac ggatattttt
       61 tagcggaaag tccgattcaa gtggttttta ttcagctgat atttataaga taagtgaaaa
      121 atcccagcaa tcagtagcgt aatcgtataa gcgattgaat gatcgaaaaa agaatagtaa
      181 ttgattgtcg ctaataagca taatgcaacc atgtaactaa tgtgcaagcg tgtaaccagc
      241 cctttttgcc cgattttgtc aaaatactgc gaccattttt cccgttgact ccctatgctt
      301 cgttttttga attgaaagaa aagggataaa ataccgactc ccagaaaagc agcgatattc
      361 agagaaataa ggtaaataaa aaaaatcatt aaaatttcaa acatattgac acctttctag
      421 ttggatagaa cctttacaga aaaaaggcaa aaggcgggaa taatgtttca cacgaaacat
      481 tattcgcgga tttgtccatc gccataaatg atatattttg tggaagttaa ttcatttaac
      541 cccattggac cacgggcatg caacttttga gtagagatac caatctctgc accaaaacca
      601 aataccgatc cgtctgtaaa acgagtggag gcattgacat agacagccgc cgcgtccact
      661 tcatttaaga aacgttgact agccgcataa ctatctgtca cgatcgtttc tgaatgttta
      721 gtattatgcc gattgatatg tgcaattgct tcatccaaag aatcgaccac tttgacagca
      781 aggatataat ctagatattc agtatcccaa tctgtgtctt ctgctaaagc agccgtctct
      841 agatattcta acgcacgagc atccgcacgt aaagagaccc cgtgttctac cagttctttt
      901 tcaatagctg gcaagaaaaa aggtgcgatc tcagaatgga tcagcaatgt ttctgccgcg
      961 ttacagacag atggacggga agcttttgca ttgacgatga tgttcactgc catagataac
     1021 tgtgcttctt tatcgatata tacgtgatta tttcctgtac ctgtttcaat gataggtacc
     1081 gttgcctgtt ctttgactcg ttggattaat tttgctccgc ctcgaggaat cagcacatct
     1141 aagtattctg tcaatcgcat caactgtcct gcagtttcat gagaagtatc atcgacaaac
     1201 tggatggcat aaggagattc gccagcactg accagtactt gctgcaataa ttggactaat
     1261 aattgattcg agaagaatgc ttctttgcca ccgcgaagga taacggcatt ccctgatttg
     1321 aatgctagac tagctgcatc tgtcgtgaca tttggacgag attcgtaaat gatgccggtc
     1381 acgcctagtg gcacgcgctt tttgccgatt ctcaatccgt cggtgttcgt ccacatctca
     1441 tctacttttc caataggatc gtctaacgaa gcaatttttt cgatctctgt agccatggca
     1501 ttgattcgct caggtgtcag ctgcaagcgg tcttgcatcg tttcactgat attgttttcg
     1561 actgcttttt ctaagtcttt atgatttgct tcaatgatcg cttgttgatt ctttttgatg
     1621 gtttgtgcca tttgaacaag tagctcattt ttgcggtttg taggcataag tgctagttgt
     1681 tgcgcgctat cttttgcctg ttgtcctaat tgcatcaagt cagtcatttc aagtcctcct
     1741 taaataaagt acctacttct tttcctgcaa gaatatcaaa aataattgcc ggattttttc
     1801 catttgctaa aatcattgcg ctgtttgctt caaatatcct agaagcggct tttaatttac
     1861 tctgcatacc gcctgttcca aatgtgctac cagcaccacc tgcttgtgcc agaatctttt
     1921 catcgatttt atcgatatag cgatacatct gagcctcggg atttgtagtc ggattatctg
     1981 aataaaaccc gtcgatatca gaaaggacga tcaaaaggtc tgccgcgaca agttttgcca
     2041 tgatggctga cagctgatca ttatcgccaa attttgtcag atgatctaat tcatccactg
     2101 ctacagtatc gttttcgttc acgataggga tcgtgtccat aatcaaaagc tgttccaatg
     2161 tattcctcac atttttacga ctttcaggat aatctaccac gtctcttgtc acgaggatct
     2221 gtgccgtctg ttgattatat acactgaatc tttgattata gatattcatc agttcggctt
     2281 gaccaacagc cgccgcagct tgctgttcag gaatcaaagt cggacgttct gacaaatgta
     2341 atttatgcat accaacgcct acagctcctg atgagactaa gatcacttcc tttccccgat
     2401 ttctaagatc agaaaggaca aaagctaact gatcgattgc cgcaagattg atatttccat
     2461 tgggataaat caatgtactt gttcctactt tgacaataat tcgttttgca tttttaattt
     2521 ctttacgcat gtgacacctc aatcaaatat ccactcatta atttcgttct attctacttg
     2581 ttttgacgag taaagtcatg taaaaaatag tttttttgta caacttgcag tcgtttggaa
     2641 aaagaacgaa ttaatggttt actttaagat agcagaattt cttgaagaat ttgtaaagaa
     2701 agtcttttac agcctcggat atgttagaat aggagtagt
//
LOCUS       DACEMS010000185         2696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEMS010000185 DACEMS010000000
VERSION     DACEMS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.194"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..935
                     /locus_tag="IW735_002625"
     CDS             <1..935
                     /locus_tag="IW735_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1045581.1"
                     /translation="ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVD
                     VSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASE
                     GIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNK
                     PQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIV
                     TATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPK
                     TGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1069..2412
                     /locus_tag="IW735_002626"
     CDS             1069..2412
                     /locus_tag="IW735_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1045582.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2524..>2696
                     /locus_tag="IW735_002627"
     CDS             2524..>2696
                     /locus_tag="IW735_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1045583.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1079 a          348 c          495 g          774 t
ORIGIN      
        1 atgcagataa atacaatcct gaaggacaaa aagtgactac agacttaaat aaagagccag
       61 atgcatctga gggaattaag aacaaagaag atttaccaaa aggaactact tatgcttgga
      121 aagagaaagt agatgttagc acagctggaa ataaaaaagg tactattgta gtgacatatc
      181 cagatggatc atctgatgaa gttgaagtag acgttacagt gacagatact cgttccgatg
      241 cagataaata taatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      301 catctgaggg aattaagaac aaagaagatc taccaaaagg aactacttat acttggaaag
      361 aaaaagtaga tgttagtaca gctggaaaca aaaaaggtac tgtagtagta acatatccag
      421 atggatctaa agaagaagta gaagttacta tttctgtagt agataaaaaa gcaccgaata
      481 aacctcaagt tgatcctatt acagagggtg accaaattgt tactggtaaa actgaaccaa
      541 atgcagaggt gacagttaca ttaccagacg gaagtcaaca ccatgggaca gctgataaaa
      601 atggtaattt tacagttaaa gttcctaaat tagaggcagg gacaaaagtg atagtaactg
      661 caactgatga atctgggaat actagtgaac ctactaatgt agttgtttct tccaacaaaa
      721 aagacagtgg aaaaaacggt agtaaaggta gtaagactga caatcaaggt agcaacagca
      781 atcaagacaa aaatagaggc aaaagccagt cctcaaaagt ttttcctaag acgggagaga
      841 gtgacagtaa tatctttact attagcggtg gtttaatact gttaggaacg ttaggattat
      901 tagggtataa aaatcgaaaa aaagagaatg aatagaaaat caatttctta agtcttctct
      961 gagaagagga agaatctttt tcttcctctt tttgcttttt tatgatggga aataagtgtt
     1021 agttaaagat gaatatactt tttatagaaa ggaaaagaat gtaattaagt ggaaaaaata
     1081 gtaattattg gttcaaacca ttcagggcta gccgcagctc gatttctaaa cttaagcgat
     1141 tcaaattatg aagttattct aatcgataaa aaatcagaaa ttagttactt ggaatgtggg
     1201 acatcgttat ttataaaagg agaagtaagt aatttagaag atctttttta tcaaaataga
     1261 atgacactaa attctgagat aaaaaagtta tttctagaga cagaagttga caaaattgat
     1321 attcaaaata aaaagattag ttgtgtaaaa aaagatggta caagattctc tcaagaatat
     1381 gacaaagtta tattagctac aggttcaaag caaattatgc tagatatacc ggggaataat
     1441 ttagctaata ttttttcgat aaagaatttt ccaaatgcaa aagagatttt taaagctttg
     1501 gatgatagta ctattaaaaa agtaggaatt attggagcag gatatattgg agttgaaatt
     1561 gctgaagcca taagaaaaag agggaaagaa gtatatcttt tcgatgttgc agatagagtg
     1621 ttgtcgactt attacgaccg tagtttttca gataaagttg aagaaatact atcaaaaaat
     1681 ggaatacatc tttgtctatc agaaagtcct aaaaaatatt taggaagaaa aaagattgaa
     1741 aaaatagtca ctacaagtgg tacccaatac tctatggata tggtcgtaca agctatagga
     1801 tttctatcta atagtccaat tggggaaaag gaactcttga gagatgttag tggagcatat
     1861 ctaactaatg aataccagca aacaaatata aaagatatat atgctattgg ggattgtgct
     1921 acaacttttt ttacgtccat taatagaagt agtgttgatt tttctatttc taatgctcta
     1981 agaacagctt atattgcaac tcaacatatt aacaatcaag attttacacc ttctggttcc
     2041 caatttacaa atggcttttc tatatttaat tacaattttt acgcagcagg tttgaattta
     2101 aaaacagcac gattattaag tgagtctata gattatatag aaatagaaga tttcattaga
     2161 cctgcttttt tgagaaaaaa tagtcgtgta aaattaagaa ttagctttga aaaaaataca
     2221 aagagaatag taggtgtaca actttgtagt caagaagatt tatctggact attatcaatg
     2281 ttttctcttg ctatagaaga acaaattaca ttagacaaat tgaaaatgtt agattattta
     2341 tttcatccta gttttagcca accctataat tttattttaa aggcagtaac aggattaaac
     2401 attgaaacat aatcgttact aaatgaaatc aagaaatata ctgtattact caattcattg
     2461 aaaatattta tcataatttt taatagaaaa cactattaaa gagaaaaaag agaggtaaat
     2521 caaatgaaaa aatctttaaa acttgcatca gcagttatat taatgtccca actccttata
     2581 gcaacacctt tccatgtgtt ggcaagcgaa aattcaacat acaaaattgc aaaagaaaga
     2641 accgagacga ctgaaatgag taattccgat gtaacagcag agagtagtga actttt
//
LOCUS       DACEMS010000186         2650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEMS010000186 DACEMS010000000
VERSION     DACEMS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.195"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            36..197
                     /locus_tag="IW735_002628"
     CDS             36..197
                     /locus_tag="IW735_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045584.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IW735_002629"
     CDS             210..713
                     /locus_tag="IW735_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1045585.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IW735_002630"
     CDS             773..1162
                     /locus_tag="IW735_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1045586.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="IW735_002631"
     CDS             1149..>2650
                     /locus_tag="IW735_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1045587.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DACEMS010000187         2645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEMS010000187 DACEMS010000000
VERSION     DACEMS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.196"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            159..488
                     /locus_tag="IW735_002632"
     CDS             159..488
                     /locus_tag="IW735_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1045588.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(775..1260)
                     /gene="vanZ"
                     /locus_tag="IW735_002633"
     CDS             complement(775..1260)
                     /gene="vanZ"
                     /locus_tag="IW735_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1045589.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1413..2324)
                     /locus_tag="IW735_002634"
     CDS             complement(1413..2324)
                     /locus_tag="IW735_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1045590.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          849 a          487 c          401 g          908 t
ORIGIN      
        1 atgtttttaa cttggaatca aaaacgcact tgcaagtaag tttacttcaa tacatgaaat
       61 tgcttataat agtgtattgg aattaaggta gattgacaaa agagtgatca aataggtata
      121 cttcattcaa ataatcgttt gaatgtgagg tgagatgaat gaaaaaagtt tgccaagtga
      181 ctgtcgtaca taaagaaaaa gtaaatggaa taaaaaataa attggatcaa aaagattttt
      241 caaacttact tgtgttaggg aaatgtttca gcgatttttc tagaattaaa attttctatg
      301 ctttagaaac ctataaggaa atgtgcgtct gtgatttagc agaaatactt actgctagtg
      361 tcgctacaac gtcacaccat ttacgttttt taaaaaaaca tggaatggca aaatcacgtc
      421 aagatggaaa agtagtttat tactctttag cgaatgaaga catcctttcc gctattcgtg
      481 ttttttaaag ttatcagaaa atctatctat gaaatcttag tttgggggta ccgtcaggaa
      541 aatgcggatt tacaacgtta aggaaattta ttaaagagac caattcctca gcactaaaat
      601 tgataattgg gctttctttg ttactatagt gttaggtaag ctttttactt tatcaccggt
      661 tttcccctca cttcacacct acgggcgagt ttccccacat cccgttttcc atctaatcca
      721 attcattcaa tatctttatg tgtatgaaag atcagattgc ttgatttaga catcttacac
      781 gtaatttatt cttaaatggg tacggtaaac gagcaataat acgagcaaaa gtatacctac
      841 aaaaataata actctgtcta attttttttg attcatatgc ttattgctta aaccatataa
      901 tttcagtcca agaaagcctc caacagtatt tgtaattaca tctgttatgt ctgtcgctcc
      961 aatagcgaag ataaattgaa ttatttcaaa agtaagactt aaaaccagta caaaagcaaa
     1021 cttaggtaaa aatccgattt ctttaaaatt gacattcaaa agcaagccaa atggaataaa
     1081 gattataaca ttatctatca tctctctgaa attcccagta gcagtaaatg gagtcaagtt
     1141 aagacttctt tgatgataat taaatactga taaaatattg tattgtaatt tgaataacac
     1201 taaccagatt agtgtcacta aatataaagc tagcaatcct ctagataata tttttcccaa
     1261 tataatttcc tctaattctt ttgtttaagt tgtatcatta gtatagcaat gtcctagtta
     1321 acgtgggttt taaattaaaa agctaacgtt accccaaaag tgctagtaca ataatgctgt
     1381 tcattaattt cgttggtttc attccgccat ccttacctcc ttgaattagt atgtgttgat
     1441 ccgggaaaca ctgtcacaat tacaccgtct atattgtttc ctgatatctc ataacgaaga
     1501 ttagtcggca catgaatggt ggtattttta gtaacaggat aataaaagat ctcatatttt
     1561 tccccattta cactaacaga aatggttttt tcttctttta ggtaatccat atattcctcg
     1621 agaacgaaat tcttttcttt cataatcgca ctatgtggta aaccaacata gcgaatatgc
     1681 catggttcat attgaattcc tgttaactct gttttgtcct ctggataacg taaaatgaac
     1741 ccgtatttcc aagcattttc ttctatccac tttccttcag gggctcgttc cattttcgtc
     1801 aagcttgatc ctacatctag tgataaacct gaattatgct cactataacc tgctggtaag
     1861 gcatactcag cccccatttc ttggtaaagc acactttgct catcaaagtc tcgatagcca
     1921 ctattaataa taaaatgact aacgccaccc tttacagcat cattgaccat ctctgaaaat
     1981 ttttgtgcta tttcttttga catataaata ttactatcaa gcaacccgta tccatttatt
     2041 aattcgtcat gtttagataa attcacgata tctgacttca cactttcttg gcgaacagga
     2101 tatttactat tgattaatag cagatttcct tgataaacct gttcttctgt aattgttttc
     2161 tcttgggtat tttcagaagt cccactattt tctaaatgtt ctttgggatt ttgatcataa
     2221 ttttgaaatt cgactttttc ctgagaaaac agtgcttcat taacgtagtc ataacctaag
     2281 tatattaaga ataacaataa caataaaaaa aacaacttct tcattttcag tctcctcctt
     2341 aactatatag ttggaagtat gaaatatttc tagaatcttc cccttaaacg gcacaatctc
     2401 gtttgagatt gtgccgtttt gccccgtaaa ttacagtcaa ttatttcgcc ctactggatt
     2461 tactattatc accaatgtag atgaattttc ataagtgata cccatgctga atttaacgag
     2521 gctgaactgc ctgttgacaa tgggcggtgt gaaataccac ccctctatgc gatcagtctt
     2581 attatcaggc tatcttgccg cagcgcacgc agataatgac cgcaatatcg ctctgcctta
     2641 catct
//
LOCUS       DACEMS010000188         2625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEMS010000188 DACEMS010000000
VERSION     DACEMS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.197"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IW735_002635"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IW735_002635"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1045591.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          943 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaa
//
LOCUS       DACEMS010000189         2515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEMS010000189 DACEMS010000000
VERSION     DACEMS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.198"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(493..1785)
                     /locus_tag="IW735_002636"
     CDS             complement(493..1785)
                     /locus_tag="IW735_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1045592.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="IW735_002637"
     CDS             complement(2059..2319)
                     /locus_tag="IW735_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045593.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          847 a          463 c          380 g          825 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagtgt aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aaggc
//
LOCUS       DACEMS010000190         2483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEMS010000190 DACEMS010000000
VERSION     DACEMS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.199"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1014..1304)
                     /locus_tag="IW735_002638"
     CDS             complement(1014..1304)
                     /locus_tag="IW735_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045594.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IW735_002639"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IW735_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACEMS010000191         2417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEMS010000191 DACEMS010000000
VERSION     DACEMS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.200"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..362
                     /locus_tag="IW735_002640"
     CDS             <1..362
                     /locus_tag="IW735_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045595.1"
                     /translation="TIPTFADSTNEPTTVVDNNQELTPLDRNGIAIGNPTNDLTVTEN
                     YLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSYK
                     SAGNNWIATYSGTLTRK"
     gene            372..575
                     /locus_tag="IW735_002641"
     CDS             372..575
                     /locus_tag="IW735_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045596.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            786..1370
                     /locus_tag="IW735_002642"
     CDS             786..1370
                     /locus_tag="IW735_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1045597.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1539..>2417
                     /locus_tag="IW735_002643"
     CDS             1539..>2417
                     /locus_tag="IW735_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1045598.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVK"
BASE COUNT          857 a          369 c          470 g          721 t
ORIGIN      
        1 ctacaattcc tacatttgct gatagtacaa atgaaccaac tactgtagta gataataacc
       61 aagaattaac tcctttagat aggaacggaa tagccattgg taacccaact aacgacttaa
      121 ctgttactga aaattattta atagtttctc cattacttgc tcgagcagct tctaaaacga
      181 tacgagtgac aggaacgatg atttttgata aagttggttg gccttctaga cctcctcaat
      241 cacggaattg gaaagaaaaa agaaatggtt attggtacta tggaaaattg tatttacaaa
      301 gttataaatc agctggaaac aattggattg cgacttattc aggaacctta actagaaagt
      361 agggattttt tatgccagaa tttgtgaaca caccaaattt ttatcaagat agcgatattc
      421 taagtaaaat taatttaccg aaagaagaat cagctttaca agttcagcga actttacagg
      481 agacacaaaa aattcaaaaa tcaatttcta atctagaaga ttctgaagaa aaaagaaaaa
      541 aaagagacca aaaaacatat caaaaagaaa aataatgatt ttaaaaaaag agtgtctagt
      601 cgacattctt tttttctaat tgaaagtgtt cataaaatca cccatttctg gacactttct
      661 ttttttgctt tatttttcaa aaaaacaatt catttcatgt tcataaaccc ataacaatat
      721 ctatgttcag ttaaactata tggataaagt tatgttacaa taaaattaat gaaggagtga
      781 tttttttgaa aataggatac gcccgggtat ctactggttt acaaaatttg gatttacaag
      841 aagacagcct aaataaattt ggttgtgaaa aaatttttac tgatcatatg agtggggcaa
      901 aaagtaatcg accaggatta gaaatggcca ttgattttgt tcgctctggc gatacacttg
      961 tcgtttggcg cttagatcgt ttaggtagaa atatggaaga tttgatttca atcgtcaatc
     1021 gactgaatga gcgtggtgtg agttttcata gtattcaaga aaatattacg atggacaaat
     1081 caagttctac aggacagctc atgtttcatt tgtttgcggc atttgcagaa tttgagcgta
     1141 atttgatcct ggaacgatcc gcagcaggac gagcagctgc tcgtgcaaga ggacggtttg
     1201 gtggtcgtcc tgaaaaattg tctgaacaag atttggaatt actgaagact ttagtggata
     1261 gcggtacacc tattaagact attgcggaac ggtggaacgt ctcaagaacc accatttatc
     1321 gttatttggc aaaaatggag gagaaaaatg gaaagacaaa ttctcattag tttttgtcat
     1381 ttaatgctga aaatttttgc catttatcta gaaaagtgac aatagtatga gaattttttg
     1441 taagtgtcta ctgataactt attctcaaat atttagctat ttcttcttta caacatcgaa
     1501 catatgtttg tataatgtgg aagaatggag tgttatttat gatggattat tcaaaagagc
     1561 cagtaaatga ttacttttta atcgatatga aaagctttta cgcaagtgtc gaatgtatag
     1621 aaagaaattt agatccatta acaacagaac ttgttgttat gagtcgatct gataatactg
     1681 gttcaggatt gatattagct tcttctcctg aagcaaaaaa gcggtatggt attacaaatg
     1741 tgagtagacc acgtgactta ccacaaccat ttcctaaaac actacacgtt gttccaccac
     1801 gtatgaacct atatatcaag cgaaatatgc aggtaaataa tattttcaga agatatgtgg
     1861 ctgatgaaga tctactgatt tactcgatcg atgaatcgat tttaaaagtg acaaaatcac
     1921 tgaatctttt tacgactgaa gaaacacgaa gtcagcgaag gaagaagctc gctcaaatga
     1981 tccaagaacg tattaaagaa gagttaggtt tgattgctac ggtaggcgtc ggcgataatc
     2041 cccttttggc taaattagcc ttagacaatg aagcaaagca taatgaagga tttattgcgg
     2101 aatggacgta cgaaaacgtt cctgagaaag tatggaatat tcccgaaatg acggactttt
     2161 ggggaattgg ttcgcgaatg aaaaaaaggc tcaaccaaat gggaattttg agcattagag
     2221 accttgctaa ctggaatcct tatacgatta agaatagatt aggtgtgatt ggattacagc
     2281 tgtacttcca tgctaatggg atcgatcgaa cagatatagc aatcccacca gaaccgacaa
     2341 aggaaaaatc atatggaaat tcacaagtgt taccacgaga ttatactaga agaaatgaaa
     2401 tcgagctggt ggtaaaa
//
LOCUS       DACEMS010000192         2414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEMS010000192 DACEMS010000000
VERSION     DACEMS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.201"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            152..424
                     /locus_tag="IW735_002644"
     CDS             152..424
                     /locus_tag="IW735_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045599.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            869..1048
                     /locus_tag="IW735_002645"
     CDS             869..1048
                     /locus_tag="IW735_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045600.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            1107..1463
                     /locus_tag="IW735_002646"
     CDS             1107..1463
                     /locus_tag="IW735_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045601.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          869 a          335 c          460 g          750 t
ORIGIN      
        1 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
       61 gttcctatac aacgaaacac gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc
      121 tgtggaagaa atgattgaat aaggtgtcga aatgaatctt attgcgacgt actatcgaac
      181 actggaagaa ctgaaaaagc aaaatgcaaa atggtttttc caagccttac tatgcttgga
      241 agtaggtgtc aaaccaagca ctattaaacc ttctgaatac caagctttag aactaacgta
      301 tgcgaaattt atcgaaacga aaaaagccaa gactgtttca agtgaatggt tagattattt
      361 tgaaaatatc aataagtatg gcgcatatta caccatgaaa aaggaagaca acgaaaatga
      421 ataaggtgat ttatcaacta acaacggatt ccatatcaac gatttgaaag aaaaaaaggt
      481 caataatcat ttataccgaa aagtgagaat gtgtgaaaca aggacaaaaa acgcctagta
      541 actaaggaaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg
      601 tgcgaaataa acgggcaaag cccgataact taagagaaag aaggtgatcc atttgaacaa
      661 gattattccc ttattgttag tactgtttgt tggcgaaata cttatgcgag atagtgcagc
      721 tgtacagtta aacctcgctg tatttttggt aatactggtg ttttgtttga tcttttggcg
      781 gtttgtatta gtcctcgtga gtctactatt caagatgatt gcagccatat ttttcatagg
      841 gttatttcga tgaaaggagt gagtaccaat gccaattgca ataggaaata aacggttacc
      901 cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa
      961 aagtgaatct aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg
     1021 atttgatgta ccagcgctaa aaaagtgacg tcacttttta atttgatcta tataagtgat
     1081 atcactttta ccaaggaggg aattaaatgt taaaaactaa aaagaattcg tacaaattaa
     1141 agcttagtat actatctatt atttgtcttt taggcatgat aattagtggt catttgtata
     1201 gagtggcata tacgcagttt attgagtaca aggttcactc tttaggatta gctggggatc
     1261 gatacgttga atatgtccat tattcattaa aatgttcttc atatattctt ctttggctta
     1321 ttttttcatt gtttgaattg ctaaatttaa tagcaatatc ttcatatcac ttagataagc
     1381 taaataccaa atctagccgt tataacgaag tgtttaagga aaatgtgaag ttagaaagtg
     1441 acttaaacaa gctaaaaaaa taaaaagaag aaaacataag ttaactaaaa ttattttatt
     1501 aagaatgatg gaatataagg ataaggttac caacataacg atcattactt ctctgatatg
     1561 ctatggactt ctatcaatca gtattttgtc tatagattat ggagtaacta tactgcaagc
     1621 cttagttttg gctatgccta tgtatctatc tttgtttatc atgcatgaaa tatataagct
     1681 atttgtttta tcaaaaaatg tagaaatacg ggtaaatatt ttggtgaata gttatcaaaa
     1741 agaactcaaa gaaagaagac ggtggcggta tttattgaaa ggatgaaaaa gtaggatttg
     1801 acatactggt attcagaaag tgccgtcact tttaaaacaa tggaaaaggt ggagataaga
     1861 tgaagaaaaa acaaactaaa ctatcattag cagatattta acacagttaa ccgcaactga
     1921 agatggtgtt gttgaatttg aacaatctga aattgcggta gtagatgatc gttaccttaa
     1981 aatgccttac ttctttgacc aagcaaaggt catttgcctt tgtcactacg atggggtacg
     2041 tgattttagg cgttaaaaag tagtgattat atcaacttta cgagtaaata taaatttaga
     2101 catgaaactg aattatccaa tggaaaatca tggtttataa tctagaaaaa ttatagtaat
     2161 gattggatta tatatattct ttcgtatttt cgtacaactg tagcttgtca aaattgagaa
     2221 atttaaccga ttaggtactt tacatcgtta tttgggtaat ttctaaaaca tgaagcttac
     2281 caacgaataa actgagacta tcttattaat tgatcaatat aagaaaatat ctttgaggag
     2341 atgatgtact atgttttcgt ctatccacga ttggttaggt ggtcttttcg gcggtggagg
     2401 taaaggtttt taat
//
LOCUS       DACEMS010000193         2399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEMS010000193 DACEMS010000000
VERSION     DACEMS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.202"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            206..2068
                     /locus_tag="IW735_002647"
     CDS             206..2068
                     /locus_tag="IW735_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1045602.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2399
                     /locus_tag="IW735_002648"
     CDS             2068..>2399
                     /locus_tag="IW735_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1045603.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DACEMS010000194         2342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEMS010000194 DACEMS010000000
VERSION     DACEMS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.203"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1368)
                     /locus_tag="IW735_002649"
     CDS             complement(join(<1..795,795..1368))
                     /locus_tag="IW735_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HAQ1045604.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIK"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="IW735_002650"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="IW735_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1045605.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          695 a          444 c          448 g          755 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 at
//
LOCUS       DACEMS010000195         2318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEMS010000195 DACEMS010000000
VERSION     DACEMS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.204"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            106..288
                     /locus_tag="IW735_002651"
     CDS             106..288
                     /locus_tag="IW735_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045606.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            272..457
                     /locus_tag="IW735_002652"
     CDS             272..457
                     /locus_tag="IW735_002652"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ1045607.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            479..604
                     /locus_tag="IW735_002653"
     CDS             479..604
                     /locus_tag="IW735_002653"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1045608.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            700..2220
                     /locus_tag="IW735_002654"
     CDS             700..2220
                     /locus_tag="IW735_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1045609.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          799 a          394 c          475 g          650 t
ORIGIN      
        1 aggcaaaata ttaaattaaa tgtaagcgtg tcataggata gtatctcgta cgccgattat
       61 ctttcttcta taattaaact atcaaatata gaaggagaaa aacgaatgaa taaaaaagat
      121 attgtaccag ttcaattttc aagtgcggct caaaaatcgc aaattgacac caagccaaaa
      181 ctagcttgca gaatcaaatc ggaagataag gaaatattta tttataatgg aatggataaa
      241 tatcttttgt ataccttgtt aaaagagttg aatgccaatg ctcattgatg caactgcatt
      301 gaaacatatt tttatcgttt gtggaaaaac agatcttcga aaaggaattg atggactagc
      361 agctttcgtt atagaagaat atgaccttga tgtttacgat caagcgcttt tcttattttg
      421 cggtacgaag aaagatcgct tcaaggctct ttactaggat caaaatggat ttgtattaat
      481 gtacaaaaga tttgaaagtg ggtaccttca atggccgcgt tccagaaaag aagtcttact
      541 gactggcctt tcaatcgaag aaaaatgtac gctaaaagaa acgaaaaaag gtgcgatata
      601 ttagacgaaa accttgttaa atcaaggatt ttgtctgttt ttcgttgtca taaaatggta
      661 taatagatgt aagaaaatcg ctagaatggt ggtgtaaaaa tgacaaaaat ggaatctgaa
      721 cttcttgtac aaatcaagca attaacacag ttgcttgaaa tccagcaaca ggaaaatact
      781 cttcttagag aacaaattgc tgaaatgaat cgacgcttgt ttggacgaaa aaaagaaaca
      841 ccacccgtag atgggcaaat cgatttatta gacgactcaa cttttaatga accagagcac
      901 actggacaag aaagccagga acccattacg gtatcttctt ttaaacgcag aaaacgcaag
      961 ggactcaaag cactttcatt agaaggtctg cctgaagttc ctattcatta tgaattaaaa
     1021 ggtgccgatt gtttgtgtga agactgtggt catactttac atgatatcgg tgcgacaagg
     1081 cttagagaag agccattgta tgttcctgcc catatagaaa aacaagtcat ctaccaacat
     1141 gcctatcatt gcagagaatg tgaagcatta ggagaaacaa aaatcaaaaa agcgcctgtt
     1201 cctaaaccgt tgatcaataa tagtctaggt tcctcttcaa ttgtgacaca gagtatttgg
     1261 gaaaaatttg ttaaaaaggt gccggcatat cgacaagaaa aagaatggca tgcacttggt
     1321 ttccctcttg atcgtaaaaa aatcactaat tggcatatca aggtaagtga aatggtttta
     1381 gagccaatct acaattgcct tcatcaagaa ttaagcaatc aggaggtctt acacgcggat
     1441 gagacaagct atcgggtatt gaatactcat aaagaaaaga ccttttactg gttatatgct
     1501 tcaggtaaaa gcgaagaaaa acaagttgtg ctctacgagc acgcaaattc gcgttccgtt
     1561 gatgagccaa aaagtttttt agaatcattt agcggtttct tgcatactga tggttacgcg
     1621 gcctatggaa agctgccggt aaacaatgtt tattgttggg cgcatgttcg cagaaagttt
     1681 tttgaagcat tgggtaaaca accgtcccca aaaagtcatg ccaaaatagg attagacttc
     1741 tgtgacaaga tgtttcgaat cgaacggacc tttaataagc ttagcgcaga agaaaggttt
     1801 gtagcgagga aggaaacgtt gcagcctgtg atgactgaat tttttcattg gtgtgagagt
     1861 tttgcttatt tgccaaaatc gcagcttgga aaggcggttc aatacgcagt caatcataaa
     1921 gacggacttc agattgtgtt gcttgatgga cgcttggaac tttcaaataa ccgagcagag
     1981 cgtgccatca aagagttagt gatcggtcga aaaaactggc tattttctaa aagcttaaaa
     2041 ggcgctagat caaatggaat tatattgagt attatccaga cagctgttgc gaatgggtta
     2101 aacatccgca aatatttaaa tcatcttttt acagagattc ccaatctgtc ttcgatgaca
     2161 cctgaagcgc tacgcgctta tttgccatgg aatcagcaga tacaagaaat ctgtaaataa
     2221 tataagccac aaataacctg atttgagtgt atcaaatcgg gctatttgtg tttaggtacc
     2281 attctatgac acgcttacgc tcaaaactac tgaattat
//
LOCUS       DACEMS010000196         2230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEMS010000196 DACEMS010000000
VERSION     DACEMS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.205"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..390)
                     /locus_tag="IW735_002655"
     CDS             complement(94..390)
                     /locus_tag="IW735_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1045610.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            746..1102
                     /locus_tag="IW735_002656"
     CDS             746..1102
                     /locus_tag="IW735_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1045611.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1112..2011
                     /locus_tag="IW735_002657"
     CDS             1112..2011
                     /locus_tag="IW735_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1045612.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          728 a          380 c          424 g          698 t
ORIGIN      
        1 aaagcaaatc gtagttgtca caatctcttc taaacgatgt tccatcagct gaccttcggt
       61 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
      121 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttaa
      181 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
      241 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
      301 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
      361 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt ctttttattt
      421 gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc
      481 caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata
      541 aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag
      601 aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt
      661 ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat
      721 tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt
      781 attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg
      841 tccatttgca gaaagccaag tcagttgcca attggacacg gcatttcgat tgatcattgt
      901 caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg
      961 accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt
     1021 agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt
     1081 gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct
     1141 acatgtggac atcaagaaat tcggcgaatc agagctgatg cagctttggc aagttttgca
     1201 aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg
     1261 cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa
     1321 tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt
     1381 tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg
     1441 gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt
     1501 gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat
     1561 gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt
     1621 atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg
     1681 ctggaacaaa ttaaatgcat ttttaggatc agaatcattc ggagaattac ttgaccgtta
     1741 ttcaaatatc aaacaagtag tttttggcca tacgcatcgt cgctttgaag ataaatccat
     1801 cggtcacacg atttacagct gcagaccttt tggttattat tatgaatggc agctgacccg
     1861 agattttgtc ttgaaagaac agctgttaga tagctatgaa cccatgaaat tacgtgtcct
     1921 cttgcgtgcc catcatccgg cctttcaagc ctatcaaaca caacatttgc aagaggaatt
     1981 tgaacaggcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat
     2041 accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct
     2101 ttttttgtgg aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag
     2161 tactagatgc aagcgtttat tctttttttg aacgataatc acaaaaaaaa atataatata
     2221 cattttttat
//
LOCUS       DACEMS010000197         2135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEMS010000197 DACEMS010000000
VERSION     DACEMS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.206"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(515..1129)
                     /locus_tag="IW735_002658"
     CDS             complement(515..1129)
                     /locus_tag="IW735_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1045613.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1735..2112)
                     /locus_tag="IW735_002659"
     CDS             complement(1735..2112)
                     /locus_tag="IW735_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045614.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          717 a          412 c          321 g          685 t
ORIGIN      
        1 caatcattga aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat
       61 tatctatcct tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca
      121 attttgttat atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt
      181 tgtaaagagt tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc
      241 atatggattc tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc
      301 ttataactca tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa
      361 caagcttaaa atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg
      421 ttaatcctca ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt
      481 atttttttaa ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta
      541 gctgttttgc tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg
      601 ttccgcagat cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt
      661 ggttgtccaa aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt
      721 tgatacttcg tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga
      781 tccactgagc tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa
      841 tgtcttctga tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt
      901 catagacacc tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat
      961 cccaaaattc aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac
     1021 cttgattaat ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc
     1081 ttaatttctc ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc
     1141 gcctagctat acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa
     1201 acagtagtta gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga
     1261 acgaatacgc ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat
     1321 ctacataggg gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc
     1381 attgactatg gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc
     1441 ttagcgccaa ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat
     1501 tgtaagtaat ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt
     1561 atatatatat aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt
     1621 taaaaaagat tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct
     1681 catctcaata gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg
     1741 tcttcttctc gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg
     1801 tctacctttt ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata
     1861 cagtacagca aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac
     1921 catcaaggta gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt
     1981 ttttctgacc atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat
     2041 ccaattgctt ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac
     2101 taattttccc atagtgaaca cacacctttt tttat
//
LOCUS       DACEMS010000198         2131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEMS010000198 DACEMS010000000
VERSION     DACEMS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.207"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            13..251
                     /locus_tag="IW735_002660"
                     /pseudo
     CDS             13..251
                     /locus_tag="IW735_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            253..1551
                     /locus_tag="IW735_002661"
     CDS             253..1551
                     /locus_tag="IW735_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1045615.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
BASE COUNT          759 a          372 c          387 g          613 t
ORIGIN      
        1 ttttagtttt tcttggaaat aaaaacagcg aaaatccgaa cattttcacc tctctcactt
       61 tataactttt acaaaatagt acttctcata aaaagcgctc tagttaaaga aaataagaga
      121 aaaatcaaaa aaaacaaaag tttcttttac acttgtttct atattcgtta caatggttat
      181 gggaagtttt taaaaaggtt aagaaaagct tacgggggtt tttgaaagca aaaagtagta
      241 ggggacgata aaatgaaaca tacacacaaa atcatattgg ctgtcacttc tgtatgcact
      301 gttattattc taggtgtaag tatctttttt gttacacaag cagcaacaaa caataaatta
      361 gaaaacacta gtcaaacaac tcaacactca agcagcagct caaaacctgt tgaagataaa
      421 caaacgacga aacagcttga tcaagcaaaa cagcttgctg caagttatca ttatgatgaa
      481 gccatcgcct tacttgaaaa agacgacgcc aaagaagcgc aacaattgtt agcaactttg
      541 aaaaaagaaa aagaatcctt ggtaaaatgg gaagacccga ccaaaatctc acacgtcttc
      601 tttcatagtc tgatcgttga tccagctaag gcttttcata cccagcaagc acaagggtat
      661 aaagattata tggtcactat ttccgaattc aataaaacca ttgatcagtt atataaaaat
      721 aactatgttc ttgttaatct aaatggatta gtcaaaaaag gaacagatgg aaaactgacc
      781 tttacaggtg tttctcttcc agaaggaaag aaaccgttaa ttctttcgca agacgatgtc
      841 agctattatg aatacatgga caattcagga tttccaagta agttgattgt cgataaacag
      901 aaccaaatca agaatattta tatcgacaat aagaaggaaa ctgttggtga ctatgatatg
      961 gttccattga tcgattcatt cattaaaaaa catcccgatt tttcttatca aggcgccaaa
     1021 ggaacattag ctttaacagg ctataatggt gtattaggtt accgtacatc aaaatcagaa
     1081 tatggtgata acgaaaaaac acacaaagaa atcgaagcag ccaaaaaagt tgctgatcaa
     1141 ttaaaggaag atggttggag ttttgcttct catacttggg gacacttgaa tatgacacaa
     1201 gcttctttag ccgatatcca acaagataat gaacgttggc aaaatgaagt tgcacctatt
     1261 ttaggcaaaa caaatatttt gatctaccca tttggcgctg acatcagcga ttggcaacct
     1321 tattctgaag caaatcaaaa atttgcttac ttgaaacaac aaggatttga tatcttctgc
     1381 aatgtcgatg cctcaactcc tgcatgggga caactaggaa ctgactacta ccgtaacgcc
     1441 agaatcaata ttgacggtat ccgtttcgaa gctgacctaa aaggagaaaa tccaatactt
     1501 gatcacttta tcaacgtcaa agaaatgtat gaccaaaaag atcgaggata gaggttgtga
     1561 aagaagcgtc cagctccgag caaataagaa ctagatttct aaaatagttt ctcatatttt
     1621 ggaaatctag cgcttattgc tgaggagttg cttcttgaac accgtgaata gaggttgtaa
     1681 aaagagcgtt ttgacttatt gttgaaggtc agctctttga acaccgcgtg ttaggttgtg
     1741 aaaaggctgg tctgcatcaa gcaataagaa tgaacagaga aaaacagctt cttgaatacc
     1801 gcagataggt tgtaaaaaaa ccgttttgac ctgacgataa ccagtaaaac agaggtgaga
     1861 ccattgctaa aagtagcagt ggtctcatct ctttttttgc acaagtcata tttgattgtt
     1921 tcgttatttt aatcaggata aagttctttt tttcatgcta aagagtgatt ctttcttctc
     1981 gattttcact ttcttcgttt aacttttggt aaatacttct gtcagaaaaa gctctaggta
     2041 gacacacgaa agcagataaa aagcctaact tttgcaacag tttatgctgt cgttcaaagt
     2101 taggctttta cagtttataa atagggttct g
//
LOCUS       DACEMS010000199         2111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEMS010000199 DACEMS010000000
VERSION     DACEMS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.208"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..424)
                     /locus_tag="IW735_002662"
     CDS             complement(<1..424)
                     /locus_tag="IW735_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1045616.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="IW735_002663"
     CDS             complement(533..1564)
                     /locus_tag="IW735_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1045617.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          660 a          338 c          361 g          752 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caattatatt gagaattaat ccttttttat ataagttgtt tgcttattga
     2041 tggattggat aaattaaacc atcacaacga gtggaaatta tatgaaattt ttcataaaaa
     2101 ttcatataga a
//
LOCUS       DACEMS010000200         2097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEMS010000200 DACEMS010000000
VERSION     DACEMS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.209"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            260..988
                     /locus_tag="IW735_002664"
     CDS             260..988
                     /locus_tag="IW735_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1045618.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            1018..>2097
                     /locus_tag="IW735_002665"
     CDS             1018..>2097
                     /locus_tag="IW735_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAQ1045619.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT          727 a          276 c          448 g          646 t
ORIGIN      
        1 taatcaaaga agaactaagt aactaccttt aaacggtagt tacttagttc ttctttgatt
       61 tgcgtgaaat agaggggatt tatttttctg gttacgtgat taaaaaatcc aaaaaatgag
      121 cataggaacg gttatgattt tagggtgtgt tttaatgaga tattgtgatt tcattaacaa
      181 gtgtatgata atctgttgtt gagttagtaa ataagtaata gagctaactc taaaataaaa
      241 ctattaggag gagatgtagg tgaaaaagtt taaaaagaaa atatatttag gattggttgt
      301 tggggtgagt atgtttgggt tcatggctca tactaaacaa gtaactgcag atactaacca
      361 aaaagaaagg attatgagcc aattagatga ctctcaaata gagtatgtac ccaatgagct
      421 aattgtcagt ttaaaggcta cacaaagaat ttcaacagag aaaaataaac cgcaaaattt
      481 attgggtgta aaagttatgg tggaaaaaga tttgactctt aggacaaatg aaagcgatta
      541 taataagcga gatcaagtat tattagtgaa attgaacgaa gaaattagtg tggaagaggc
      601 agtaagaaca tttaatgctt ctgatatggt caattatgca gagccaaact atgtggttga
      661 actagagccg ttgaatgatg ttgaggagcc aaatacaact gattctttag gaaatcgatc
      721 aattattctt aacgatccta attggtgtaa acaagctaat ttaagagaga ttagcatgcc
      781 atccgcttgg gaagaaacca agggttctaa ggatgtgaag gtagcggtca ttgattcagg
      841 aattgattat caacatgaag atctttctgg aaatgtagat gaaagtttgg gatacgattt
      901 ttttgacgat gatacagatc ctatggatac tatgggacat ggacacatat tgccgggatt
      961 attggtgctc aaacaaacaa cgaattaggt gttgctggaa taaattggga tgttacgatg
     1021 gtacctatac gtgtatttgg tagtagtgga ggtaccagtt tggcaatctt aactgaagct
     1081 attaattatg catcccaaaa agggtttcca attatcaatt ttagtatagg tagtgttgtt
     1141 tatagcagta cagaaaaagc aattaaaaac tatcctggat tattcgtagc agcagctgga
     1201 aataatggga tagatatgac tgataaacct ttttacccag cttctttgga tatacctaat
     1261 attattactg taggaaattc tagtggttat gaacgaagtt ctgattcaaa ttataggaaa
     1321 acaggtgttg atttattcgc tagagggttt cgtgttttta gtacatttct aggaagtaaa
     1381 tacacgtata tgtcaggtac ctcgatggca acacctcatg tcactggtag tgctgcatta
     1441 gcgttgtcac tagatcctga tctatctact gatgaattaa aaaaaatcat tttaaatagt
     1501 gttgattcat tttatgaact atctgattta tgtcttacag ggggaagact aaatacgaat
     1561 aatgtcgtac gacaagtaac gggtaaactt actgcatcta atatcacatt aagattaaat
     1621 agtgaatggt cagatgaaat tgctaaagag atgtgtcacg ttcactggat tgaccagtca
     1681 ggaaatgatt tgacggatca agtggttgtt ctaaaaaacg aagtggatac atctaacttt
     1741 ggcgattacg aagtagtttt tgcatcacct gatttaacag atcaaatctc agttacagtg
     1801 accgtacctc gattacgtaa acttacgcta ggaaaagaaa cagcaaccat tgatattggt
     1861 tccgcatggg atagtgtcat agccattgat acttttcaag tgaaagctat agatgatttt
     1921 gggaatgatg ttacaaatca agtacaggtt actattaatg ctgatataga tacaattaat
     1981 tcaattcaag cagtgattaa gtttgaagtt ccagaattag gcttagccca aattggtaga
     2041 ctgaatatta ctgcaaaaaa tagtacaatc aatggattga atgaagttag catcgag
//
LOCUS       DACEMS010000201         2086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEMS010000201 DACEMS010000000
VERSION     DACEMS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.210"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..156
                     /locus_tag="IW735_002666"
     CDS             <1..156
                     /locus_tag="IW735_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ1045620.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IW735_002667"
     CDS             175..1452
                     /locus_tag="IW735_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1045621.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..>2086
                     /locus_tag="IW735_002668"
     CDS             1462..>2086
                     /locus_tag="IW735_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1045622.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLK"
BASE COUNT          873 a          187 c          356 g          670 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaa
//
LOCUS       DACEMS010000202         2080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEMS010000202 DACEMS010000000
VERSION     DACEMS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.211"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            89..418
                     /locus_tag="IW735_002669"
     CDS             89..418
                     /locus_tag="IW735_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1045623.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            408..695
                     /locus_tag="IW735_002670"
     CDS             408..695
                     /locus_tag="IW735_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1045624.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(974..1537)
                     /locus_tag="IW735_002671"
     CDS             complement(974..1537)
                     /locus_tag="IW735_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1045625.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT          742 a          312 c          334 g          692 t
ORIGIN      
        1 gagttttatt attttgtatt tctactaaaa atagcgtaca atgtaattac taagtaatta
       61 cgaaatgtaa aataatggag gaaataatat gattacaaaa acaagaaaac aaggaaattc
      121 tattatgtta acggtcccta aagaattcga tgtgcccaat ggtgttgaag tagaagctaa
      181 gcttgtagag aatggtatcc tttatgagtt tgtagagcct aaaaaagaat tttttgattt
      241 tagtgaagat attttggctg atattctttc agaaggttat agcaaacaag agatcttaaa
      301 agaatttaga aatagaaaaa atgcgttaac ttctgcattt aaatctattg ctgaagatac
      361 tgttgcaaat tctaaaccga tgacaaaaga ggagttggca gcagaaattg gcttatgaga
      421 ttctaccttc taaacacgta ataaagtatc taaaaaaatt aaaagagaaa acattaaaag
      481 aacaattttt aacgataatt tatgatgaga tagcagttcg tcctcattcg ggagaacaaa
      541 aaacgggtga tttatcagga atttgggcta ttggattcaa gtatgcaggt acgacatatc
      601 gcgttgcata tgaaattaaa gataatgcag tgatacctat cttactatgt ggaacgcatg
      661 aaaattttta tgaacaatta aaaaagatta ggtaagaacc caatgtttca acttgagagg
      721 atagacgaca ctttaaaaga taatttttaa cagctgagag ttgtaatacc ctataatttt
      781 aaaccactaa cctatatatt tatgaaaaat agaaaaaatt aaaacgcatt ttaagcctct
      841 ttcaaattaa aagcttcatc cttatgcaca aagagggctt aaaatgcgtt attttatatt
      901 gttttttagt aataataaaa aaatcactct aattctccca taaatattat agattcctaa
      961 agattaaaat agattattcc ctattgtctt ttaagtagcg gtagagtgtt gccttgctaa
     1021 taccagtcat ttccgtaatt tcttttacgc tataaacctg acttttatac aattttactg
     1081 ctcgatcaac atctttctgg ttcactcgtg gacggccacc tttacgacct ctcgcacgtg
     1141 cacttttcaa accttctttg gtacgttcaa caattaaatc acgttcgaac tgactaaatg
     1201 cttggaagac agtcatcatt aagcgtccgt gaggcgtgct ggtatcgaag ttttctttca
     1261 agctcaccag cttaacatgg tgttcttcaa aataggtaac aaggtcaatc aaatcctttg
     1321 tactacgacc caatcgagaa aaactttcta caattaccgt atctcccgga cgaattttat
     1381 cttttaaacg atttagttgg ggacgaaccg ctttggttcc cgtcattttc tcagttagga
     1441 tttcattaca attttgttca ttgagtaagt ctaattgacg atttagttct tgtgaacgtg
     1501 tactcactcg ggcataacca taaatgtatc cactcatgct tttcttcctt tcgtacttta
     1561 attctggctt gattgtatca taaaagggag ttatagatac atagttaatg caacaagtta
     1621 tgatacaaaa tatgacctct tttatttaat aaaagaggtc atttcatttt gtctcataat
     1681 cgattgtttt tgatacgagt gtgtatacga aataaacaaa aaaatcttaa gtaaatttct
     1741 agatacctaa tttaatccta actagtgtat cctaaagtta ataacgagtt tatcacaatg
     1801 aaaattataa aatgctccca tattagatat aacatgcaat atttgaatta gaactacaaa
     1861 atttattcgt tttataaagt aaataatgtt gagatactaa ggaatagaat attataagga
     1921 attgagctga atgtattgga taaaaaaaga ataactttat tagagatgga ctataaaaat
     1981 gctcgtaatt ttcttctgaa gtctgaaagc tatttgaatg ctcagctacc agaatatatt
     2041 agttttgacg aaactttaaa atctgctaaa aaagttttaa
//
LOCUS       DACEMS010000203         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEMS010000203 DACEMS010000000
VERSION     DACEMS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.212"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW735_002672"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW735_002672"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1045626.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IW735_002673"
     CDS             complement(1431..1823)
                     /locus_tag="IW735_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1045627.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACEMS010000204         2025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEMS010000204 DACEMS010000000
VERSION     DACEMS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.213"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            297..1337
                     /locus_tag="IW735_002674"
     CDS             297..1337
                     /locus_tag="IW735_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1045628.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          767 a          275 c          326 g          657 t
ORIGIN      
        1 aggtagttct taatttattt tttgttttga cactggtagt acttaattat ctttaatttt
       61 aaaactaaat tttattcata cgtttttctg gtagtattta attataaata atatttattt
      121 aaagtagttc ttaatgactt taaaggtagt acataattca ttttatttct gattttaggt
      181 agtatttaat taaaaaacag ttttaatgcg tttgcatttt ttatttatct ttgctatttt
      241 cgaaaagagt aggtaatttt acataaataa aacaacgtga aagtagagga tctacaatga
      301 ctgattttaa attttttaag gcggatagag tttacaacga attattttat caatttccaa
      361 aagtctttat tgtttctgac gaatacaaaa aaatgaaaga ttcaactaag attgcctata
      421 tgcttttaaa agcaagatta gagatcgcaa tcagcaaacg gcaaatcgat gaagaaggta
      481 atgtttattt tacttatacg acaaatgaac tatgtagagt attaaactgc caaaaacaaa
      541 aagcgatagc aatcaaaaaa gagttggaat cctttggttt attattacaa aagcagatgg
      601 gatttaacaa acagttaggg aaaaataatc ctaatagact atatctagca gaattaaaag
      661 tctcagaaaa tgatatctac ttactcgaaa aatttgatag agagaatagg gaaaacgttg
      721 ataaatcaga gggtatgaaa atcataccca ccctcgacga aaaatcagac gctgaatccc
      781 ttggggctca agagggtatg aaaatcatac cgtgccaaaa cgttgataaa tcagagggta
      841 tgaaaatcat accagaactt aataataata tattagacac taatagacac aatatagaca
      901 ctgaaaaaga ccgcctacaa gatcaattgt tgttagacaa ttttgagaca attatgacaa
      961 acgacagcat tgctacgttt gtccctgaac gagtattaaa tttgataaaa acattttctt
     1021 caagttacag tgaagctcaa aaaaccgtcc agactattca taatgcaaag aaaaaagctg
     1081 aaatagaaag tggtatttcg atagtttttg aagaactcga tagttattat gtcaatgcag
     1141 aacaagaatt atacacgaca ctgttaaaag cctatcaaaa attaaaaacc gaaaaagtcg
     1201 aaaatatcca gaacctgatt tttgtctatg taaaaaattg gtttatcgaa aaaccaatag
     1261 ctgctaaagt atcaagtgaa aaacgtttga attatgaaag ctccccaagc actattacga
     1321 aagactggtt agagtgaaac tttactgaca aagtgattgt tgcttcatgg agtaacatta
     1381 aaattaaaaa ttttttatta ttctaattat attacgaata ttacaaaata atagctattt
     1441 taatagaatt tataagttaa aatattatga taaaaatcac aaacgtagcc ttttacgtca
     1501 taagtgttaa aagaaataat taatttcaaa tatagaaaag ggaacgtatg tttgcatact
     1561 cctttttttt gagttataat aatacgcgaa gagagataat ttcattattt aaacaagcta
     1621 aaaatcttga tattacttct tttcaggcaa tacgaatatt attcgcttac attttttgta
     1681 taaatttaat tgtctgctat gcttgtgatg aattgatttt cgttctgcac ttttggatga
     1741 taaatttgga caattgaaaa tacaaaaaaa agaaaggcgg tatgaactcg gaaggctggt
     1801 aaccttcgtc agcgttcata cacggcatta agatttggcc cttaatgcgt taacctttca
     1861 gtgttacggt attacccgca atggatttag tataacagat attcaaaagt gataccactt
     1921 tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcaca
     1981 atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgc
//
LOCUS       DACEMS010000205         1955 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEMS010000205 DACEMS010000000
VERSION     DACEMS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.214"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            201..1166
                     /locus_tag="IW735_002675"
     CDS             201..1166
                     /locus_tag="IW735_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1045629.1"
                     /translation="MSKNGTIVNEWENLTALSNRRLNVKTFYTPLKIRWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKINQFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERKKAVVPKEIETWWRLELQ
                     LRRGKATDWHKMVHESLDSFASPHFLPIDTKHTDRIMIAGLMSDHSLWSGLTRPTKYK
                     YRNLLKQESQNDELTNHLRETFSESADNLKQELDTWLLGLDVTEK"
     gene            1366..1568
                     /locus_tag="IW735_002676"
                     /pseudo
     CDS             1366..1568
                     /locus_tag="IW735_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          645 a          293 c          433 g          584 t
ORIGIN      
        1 aaaaaaatag ccgattggtg ttggaagcac ctttcgacta attacggaaa ctatcgcact
       61 agctccgtaa ctcataatca ttatacgaat atttttaggt ttcttcaaga ctagagattc
      121 ttgttaatga attttaaggg ttacgtgaac ttttaaaatt ctttccaaga atctcttttt
      181 tctttgagga ggaacgaaat ttgagtaaaa atggcacgat tgtaaatgag tgggaaaatt
      241 taacagccct atctaacagg cggttaaatg taaaaacttt ttacaccccc ttgaaaatac
      301 ggtggtcaat tgaccgaatt accattgttg gaaaactgaa agaaaatatt tattatcata
      361 ctcaaaatga tgtattgatt ttagactttg aacagttaat gcgactgaat gaaaataatg
      421 gttatgttaa atctgttggt ctaaatggtt ggcaaattgt ggataagcat gatgagaata
      481 ttgcttacat tgaaattttg aaatatcaag aagggcaagg gcgaattgac tttaatccga
      541 ataaaattaa tcagttttta gctagctcga tgaagaattt tatccatgat ttatttttgg
      601 agccgcattt ttcacgtgca gatattgctt gcgatataat tgacgtacca gatgatttca
      661 ttacccaata tcgggtggtt gatcctgtat cctttaaacc aatttatggt cggtctggca
      721 agttagaaac tgcttattgg ggcagtcgtg ctagtgaacg tcaagttcgt atgtacaata
      781 aaaagctgga acaggaacga aaaaaagcag tcgtgccaaa agaaatcgag acttggtggc
      841 gtttagaatt acagttgcgc cgaggaaaag caactgattg gcacaaaatg gttcatgaaa
      901 gtttggatag ttttgctagt ccacactttt taccaataga tacaaaacat acagatagaa
      961 ttatgattgc tggtttaatg tcagaccatt ctttatggtc ggggttaact agaccaacta
     1021 aatacaaata tcgaaatttg ttaaaacaag agagtcagaa tgatgaattg acgaatcatt
     1081 tacgagaaac attttcggaa tcggcagaca atttgaaaca agaattagac acgtggcttt
     1141 tggggttgga cgtgacagag aagtgatacc gaataagaaa agtcagatga gtgagagtgg
     1201 atttgcgtca atgtttagtg acgtaaattc acgataagcg aatattttgc atgaagcaaa
     1261 ataaagcgta aaaggagttg agagcgtgac acgctttcgg ctcctttttt cgttgtcact
     1321 tgtgacagcg aaaaccctcg gagagcgttt ggaattgtga cagccatgtc acaattagga
     1381 aaatgttact ctttgacaaa gagtaacaga aaggtttaca ctctttttat tagcgacagc
     1441 cgaaggaggg ttttaacttg gggttcagta tttcgttcaa aaagggaaca aagaatacaa
     1501 gcatttctca taataatcgt gagttaactg atagccaaaa aatcaatggt ttgtctggta
     1561 gaaaataatt agcagattta caagtgggaa ataaaaagct gaaagaagaa aattgcattt
     1621 caaactacat tttctacaat agctctaaaa cggcatattt tgcgttctga gcggttttag
     1681 cgtttatttc gtttagttat cggcacaatc gttaaaacac gttagaggag cgagaatgga
     1741 gcctgtaagg cgactggtag cggaacggtc gtgttagatt gtgaaagccg atagctgaat
     1801 gaaataaaca gcgggtgcgc ccttccattt cggcaggagg aggctcaagg ggtttgggga
     1861 atgacattcc ccaaggtctt gtgtctttta tactcgggag tatttgtcag tttttgtttt
     1921 ctggtgttat aattatcaaa aatcgaaaaa agagc
//
LOCUS       DACEMS010000206         1937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEMS010000206 DACEMS010000000
VERSION     DACEMS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.215"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..906
                     /locus_tag="IW735_002677"
     CDS             4..906
                     /locus_tag="IW735_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1045630.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            938..1180
                     /locus_tag="IW735_002678"
     CDS             938..1180
                     /locus_tag="IW735_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1045631.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(1436..1807)
                     /locus_tag="IW735_002679"
     CDS             complement(1436..1807)
                     /locus_tag="IW735_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045632.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT          750 a          320 c          379 g          488 t
ORIGIN      
        1 tccatgaata acaataacaa aaataataac aataaaatga acaaccaaaa accagtgtct
       61 gcacatccag acccaacgac accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa
      121 acagcaccag tatctgcaca tccagatcca gtagtcccta acgttcaaca aggagtagaa
      181 gagaagaaac atgatgcaga aaagcatgca cctcatcatc caggaactcc agaatctaaa
      241 gttccagaac aacatgatgc aatcgatcct tctaaacctg tggaagagca aaaacatcct
      301 tctgaccatg cgaaagcaca agaaaaactt agttctgacc acacaaacgg acaaggaaaa
      361 cttggttctg atcatacaaa taaccaagga aagcatgatt ctgaacagga agattcaatg
      421 cgcggtgagc tgaattatga agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa
      481 caagtagacg gtttattatc tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt
      541 gtgaatacag ataatgtaac ggctgggatc gaaacagaag tcggtaaaaa acaagtagca
      601 gttgatatgg atgtcattgt ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg
      661 caagaagtaa tcggcaaaga agtaaaaaat atgacacatt tagaagtgat cgaagtcaac
      721 gctaacgtag tggacatcaa aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa
      781 gacaaagtaa ctgacgcagc gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa
      841 gtaaaatcag cagctggaag cggcgcacaa aaagtaaaag aaaatacaga accacgggtt
      901 cagtaataag gaagtaaaac attaggagga acgaaacatg ggatttattt ggtcacttat
      961 cgtcggagga attatcggag caattgcagg agcaatcacg aataaaggtt cttcgatggg
     1021 gattattgca aatgtcgtag caggtttagt tggatcagca cttggtcaag cattattagg
     1081 ttcttgggga ccaagtattg caggaatggc aatcgtgcca tcattgattg gtgccatcat
     1141 tttagtacta atcgtatcat tctttgctcg aaaactgtaa aaagactttt aaaaagaggt
     1201 cgtgaaaaag ctgttcttca tcaagtaata agaacagcca aacaaaaata gcttctcata
     1261 tttttcattt ggttgggcat attatcgaag atgcaagctt ttgaacactg tttatacgga
     1321 ataagaggct gtgacaagac ttttgtcaca gcctcttatt tatgcgggca aattcccaat
     1381 tagattttta aaagtttttc cttaaatgtg tctttattga tacagagaag cttctttaat
     1441 aaaacaatgc ctccctaaat tttcttgaga taggaaaggt gaacatacaa aataatacga
     1501 ccactttgaa aacccagatg atccacaaat ggatcgtatg atactgatga agtaaaaagc
     1561 aatggatcag aaactcaaag agattgaaaa agacaaggaa gaaataatat ctctttttta
     1621 ctcttgtctg ttttagccaa taatagctcg taaagtaatc tagacaaaca aatagaagta
     1681 aacaaagttt cgtaagagca gcaatcgtat catgttgctc tgctagacga ttgacgagca
     1741 ggaaaaatgt tggtaatgga atatagatca cgagtaatga aagaaaaaag taaacaataa
     1801 atggcatact attcctccta attgtgtgag ttcgccctca ttttaaaata acgatatttg
     1861 tttgtgaagc tttttcgtgt cgtaacaaat gttatctaat gtatttttaa ataaataatc
     1921 attattatca aaataat
//
LOCUS       DACEMS010000207         1820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEMS010000207 DACEMS010000000
VERSION     DACEMS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.216"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(348..1640)
                     /locus_tag="IW735_002680"
     CDS             complement(348..1640)
                     /locus_tag="IW735_002680"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1045633.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          521 a          385 c          331 g          583 t
ORIGIN      
        1 tgaccgagga gttggcagga gaacaccgtt tagtggagag gttgtgaaaa agctgctctg
       61 catcaagcag taagaacaat cagataaaaa tagcactcca tatttttcat ctgattgggc
      121 ttagtgccgt agatgcaagc ttttgaacac cgtttgttcg gaaaaaggga ctgtgacacg
      181 gtttttgtca cagtccctta ttctgaataa acagtgttca agagacagcc tatcggcaat
      241 aaggctaaac gtctctttca ctacctcttc aagttattat tatcgtatag ataaacactt
      301 cgactcgctc tggtaagaac gtcctgattc agtcgtcgtt tttatcctta gatttgcgcc
      361 cagacgcttt ctaagatgtt tgtttgtgtt ctatcaggtc cgacagaaaa tgttgagata
      421 cgtacaccaa caagctctga gatacgacgt acatagttac gtgcgttttc tggcagttct
      481 gcaagtgttt tgcatcctgt aatgtcttct gaccagcctg gcaattcttc atagattggt
      541 ttgcagcggt tcaactcttt caagcttgct ggatagtggt agattagttc gccatctaat
      601 tcataagccg tacaaatttt tactgtttcc aatccactta atacatcgat cgagtttaaa
      661 gataggttgg taatacctga aactcgttta gaatggcgca taactacaga atcaaaccag
      721 cctacacgac gcggacgacc agttgtcgta ccgtattctc gtcctacttc gcggatttgt
      781 tctcctgttt catcaaataa ttcagttgga aatggtccat caccgacacg tgacgtatag
      841 gctttgcaga cgccgacaac tttattgatt tttgaaggcc ctacaccgct tccgattgtt
      901 accccaccgg ctactgggtt agaagaagta acaaaaggat aagttccctg gtcgatatcc
      961 agcattacac cttgtgcgcc ttcaaacaac acgcgtttgc ctgcatccaa agcatcattc
     1021 aagataacgg aagtatctgt gacgtattgt ttgatttgtt gtccgtattc ataatattct
     1081 tcaaaaatat cgtcaaaatc aagtggttct gaatcaaaca tttttacaaa ttgacggttt
     1141 ttgtcatcta gattcaaacg aagacgttct tcaaaaattt ctttatctaa taagtctgca
     1201 atacggatac ctacacgagc tgctttatcc atataggcag gtccgattcc tttaatcgtt
     1261 gtaccaatct tattttcgcc cttagcatct tcttgtaatt ggtccaattt gatatgataa
     1321 ggcaagatta cgtgcgcgcg gtcagaaata cgcaaattac ttgtttctac acctttttca
     1381 tgtaaataag ctaattcttt aaccaatgat ttaggattga ctacaacgcc gtttccgatc
     1441 acactgatct tgtctttata aaaaatccca gaaggaatca aatgcagttt ataagtcaca
     1501 ccgttaaact taattgtgtg ccctgcattg tcgccacctt ggtaccgtgc aatcacttca
     1561 gcattttcac tgagaaagtc tgtgattttt cctttccctt catcgcccca ttgcgtacct
     1621 actactacta cagatgacat gtgaacacct cgttctatat tatttaatcc tgaaccattg
     1681 tatcaataat agatacatat ttcaaataaa atcgaatgat ttttattttt attttataaa
     1741 aacgcttaaa aaacgaactt tatcttttta gaatggttat taaaaaagag gttgtaacag
     1801 aagtggtcag cttcaagaaa
//
LOCUS       DACEMS010000208         1784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEMS010000208 DACEMS010000000
VERSION     DACEMS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.217"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            294..1778
                     /gene="guaB"
                     /locus_tag="IW735_002681"
     CDS             294..1778
                     /gene="guaB"
                     /locus_tag="IW735_002681"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1045634.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          606 a          317 c          403 g          458 t
ORIGIN      
        1 tactcgaaac taagcagctt tttcacagcc tctccactaa acggtgttca aaagtttgca
       61 tctgcggtaa taaacccaac caattcaaaa acatgagaag ctgtttttga ttggctgttc
      121 ttattacttg atgcaggaca gcttttttac aatctctttt aatacacaca gatgtaaacg
      181 ctttatattg gtattctatg agaaagctaa cttgattcca agtagatcat tgacttaaat
      241 gaaaaaatca attattttaa tagataaact aaattagagg agtggtacta aaaatgtcca
      301 actgggaaac aaagttcacg aaaaaaggat ttacttttga tgatgtctta ttgataccag
      361 cagaaagtca cgtgttacca aacgaagtgg atatgagcgt acaattagct aaaaatatta
      421 agttgaatat tccaatcatc agtgcaagta tggatactgt taccgacagc aagatggcca
      481 ttgctatggc aagacaaggc ggactaggtg tgatccataa aaacatgacg atcagccagc
      541 aagcggatga agtaagaaaa gtcaaacgtt ctgaaagtgg cgtaatcatt gatccgttct
      601 tcttaacgcc acagcatctt gtagcagacg cagaagaatt gatgagcaaa tatcgtatca
      661 gtggtgtgcc aatcgttgaa acacttgaaa acagaaaatt ggtagggata attacaaacc
      721 gcgacatgcg ttttgtaaca gattatcaca tgccgattgc tgatgtgatg acaaaagaca
      781 acttagtgac tgccccagta ggtacctctc taaaagatgc agaaaagatt ttgcaaaaac
      841 ataaaatcga aaaattacca atcgtagata acgaaggtcg tttaagcgga ttgatcacga
      901 tcaaagacat cgagaaagtg atcgaatttc caaatgcagc aaaagatgaa catggtcgat
      961 tgcttgtagc agcagccgtt ggtgtaacaa gcgatacatt tgagcgtgcg aatgctttat
     1021 tagaagcagg ggcagatgca atcatcatcg atacagctca tggacatagt gcaggtgtga
     1081 tccgaaaaat tcaagaaatc cgttcaactt ttgcagatgc aacattgatt gcaggaaacg
     1141 ttgcgacggc tgaagcgaca aaagctttat atgatgcagg tgtagatgtc gtaaaagttg
     1201 gtatcggacc aggatcgatt tgtacgacac gtgtggtagc cggtgtaggt gtacctcagc
     1261 tcacagctat ttatgatgca gcatcagttg ctcgtcaata tggcaaagcg attattgcag
     1321 acggagggat caaatattct ggtgacatcg tcaaagcatt agctgccggc ggacatgcag
     1381 tgatgctagg aagtatgttg gcagggacag atgaatcacc aggtgaattc gaaatcttcc
     1441 aaggacgtcg attcaaaaca taccgaggta tgggctcatt aggcgcgatg gaaaaaggtt
     1501 caaaagaccg ttacttccaa ggtagtgtca atgaagcaaa taaattagta ccagaaggaa
     1561 tcgaaggtcg ggttgcttat aaaggaagtg ttgcagatat tattttccaa atgatcggcg
     1621 gtttgcgttc aggaatgggt tatgttgggg cagccaatct acagcaatta cgtgatgaag
     1681 cgcaatttat ccaaatgagc ggcagtggat taaaagaatc acaccctcat gatgtacaaa
     1741 tcacaaaaga agcaccaaac tattcagttg aatcttaaaa acat
//
LOCUS       DACEMS010000209         1763 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEMS010000209 DACEMS010000000
VERSION     DACEMS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.218"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1301
                     /locus_tag="IW735_002682"
     CDS             <1..1301
                     /locus_tag="IW735_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1045635.1"
                     /translation="STLSLISLSIKDATDRASKETFANITNSFSMEINRQVNPGTPRG
                     GGNVKGEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTV
                     MLTGVNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSN
                     LFDADNEKGADETVEVEIKGLFDGHNSGVVSAAQELYENTLITDIHTAAKVYGNTEDT
                     AVYQDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNK
                     LFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFV
                     GSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINV
                     LPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            1431..>1763
                     /locus_tag="IW735_002683"
     CDS             1431..>1763
                     /locus_tag="IW735_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1045636.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          674 a          230 c          323 g          536 t
ORIGIN      
        1 tgtcaacttt aagtctcatt agtttatcta ttaaagatgc tacggacaga gcttcaaaag
       61 aaacatttgc taatataaca aatagttttt ctatggagat aaatagacaa gtaaatccag
      121 gaacacctag aggtggggga aatgtaaaag gtgaagatat taaaaagata tctcaaacag
      181 atagtataga ctcctatgta aaaagaataa acagtgttgc agatttagtt gattatgata
      241 ttattgaaac tcaagagact cttgcgaatc aatcacctga aagagcaaag aattttaaaa
      301 gaacagttat gttaactggg gttaacgact caacaaaaga aacaaaattt gtatcagaag
      361 catataaatt agtagaagga aagcatttag aaaatgaaga taaaaataaa atcttaatgc
      421 ataaagattt agctgaaaaa aataacctta aagttggaga taagataaaa ataaaatcta
      481 atttatttga tgctgataat gagaaaggtg cagatgaaac agtagaagta gaaattaaag
      541 gtctattcga tgggcataat agcggtgtag taagtgcagc acaagaacta tacgaaaata
      601 cattaatcac tgatattcat acagctgcta aagtttatgg taataccgaa gatacagctg
      661 tataccaaga tgcaacattc tttgtaaaag gtgataagaa tcttgatagt gtaataaaag
      721 atcttgggaa attagatata aattggagag aatataattt gattaaaagt tcatcaaatt
      781 accctgcatt acaacaatct atatcaggta tttactcaat ttcaaataaa ttattttttg
      841 gctcattaat atttgcagga gttgtagttt cattattatt attcttatgg atgaatgcta
      901 gaaagaaaga aatagcagta ttactatcat taggtatatc aaaattagaa atttttggtc
      961 aattcctaat tgagatggta tttatatcaa tccccgcatt cgttggttcc tatttcttag
     1021 ctcaatatac agctgacaag cttgggaata atatattgaa taaggttact ggtgatatag
     1081 ctaaacaaat agctaaacaa tctgcatcta gccaattagg tggtggggca gaagctgaag
     1141 gatttaacaa gacattatca agtttagata tcaatgtatt acctaaattc atgatttatg
     1201 tagttatatt tatgagttta gtacttcttg tatcattgat tatttcttca ttcaatatat
     1261 taagaaggaa tccaaaagaa ttattaattg acaataatta aaattttcgg tgctttttag
     1321 caccaaaaat gagagtgtaa tataaaccgt gtaagtaacc atcaatccta ggcgggtggt
     1381 tgcttcacgg tttttatttt tctaaaataa gatcaagagg tgattacacg atggctagaa
     1441 agaaaagaaa tcctgatgct gagaagttag ctgaatccat tttgaatact tatcaacccg
     1501 aatctgtaga ggatatgcaa gatgttttaa aagatgtgtt tggtccctta tttgaaaaaa
     1561 tgcttcaagg agaattgaat aaccatttag ggtatgacgc ccattctaaa gagccaaaga
     1621 aacatgacaa tcgacgaaac ggttatggca ataagacgct taaaacgagc tttggtaaag
     1681 taaacattga tgttcccaga gaccgggagg cttcttttga acctgagttg attcctaata
     1741 gaaaacgaga tgtctccgac atc
//
LOCUS       DACEMS010000210         1718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEMS010000210 DACEMS010000000
VERSION     DACEMS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.219"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1307
                     /locus_tag="IW735_002684"
                     /note="aph(2'')-Ia"
     CDS             <1..1307
                     /locus_tag="IW735_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ1045637.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYS
                     YISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT
                     IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1437..>1718)
                     /locus_tag="IW735_002685"
     CDS             complement(1437..>1718)
                     /locus_tag="IW735_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1045638.1"
                     /translation="IHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIE
                     RLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          686 a          159 c          297 g          576 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata
      661 ttagtgatga attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag
      721 aaatttattc tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt
      781 ttttaagaca aatgcacggt ttagattata cagatattag tgaatgtact attgataata
      841 aacaaaatgt attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg
      901 atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg
      961 agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca
     1021 ataatagatt aactggaata attgattttg gagattctgg aattatagat gaatattgtg
     1081 attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata
     1141 tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg
     1201 aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta
     1261 tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat
     1321 gaaaagctat tataaaagac attagtatta aatagtttaa aaaaatgaaa aataataaag
     1381 gaagtgagtc aagtccagac tcctgtgtaa aatgctatac aatgttttta ccatttctac
     1441 ttatcaaaat tgatgtattt tcttgaagaa taaatccatt catcatgtag gtccataaga
     1501 acggctccaa ttaagcgatt ggctgatgtt tgattgggga agatgcgaat aatcttttct
     1561 cttctgcgta cttcttgatt cagtcgttca attagattgg tactctttag tcgattgtgg
     1621 gaatttcctt gtacggtata ttgaaaggcg tcttcgaatc catcatccaa tgatgcgcaa
     1681 gcttttgaat attttggttg atcgatataa tcatgaat
//
LOCUS       DACEMS010000211         1700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEMS010000211 DACEMS010000000
VERSION     DACEMS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.220"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            466..1065
                     /locus_tag="IW735_002686"
     CDS             466..1065
                     /locus_tag="IW735_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1045639.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1129..>1700
                     /locus_tag="IW735_002687"
     CDS             1129..>1700
                     /locus_tag="IW735_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045640.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          612 a          257 c          321 g          510 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
       61 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
      121 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
      181 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
      241 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
      301 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
      361 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
      421 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
      481 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
      541 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
      601 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
      661 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
      721 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
      781 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
      841 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
      901 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
      961 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     1021 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     1081 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     1141 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     1201 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     1261 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     1321 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     1381 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     1441 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     1501 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     1561 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     1621 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     1681 tttgacaact ttataggaga
//
LOCUS       DACEMS010000212         1675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEMS010000212 DACEMS010000000
VERSION     DACEMS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.221"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            230..>1675
                     /locus_tag="IW735_002688"
     CDS             230..>1675
                     /locus_tag="IW735_002688"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1045641.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEEE"
BASE COUNT          570 a          316 c          329 g          460 t
ORIGIN      
        1 atttaacaag gttattcgaa ccttatttta agttacttat tatttaatgt atcgaaaaag
       61 gtgaaatagg tttgttataa taagaattaa gtaagacatg aattgtctaa gcaaatgtag
      121 cttatccatt tcattaaaat acatatggtg aaaaaacaag ttcaaatagt aactaatttt
      181 ttaaaaaact gatttataaa aaatacaaca cgaatgagga aaataaccaa tgacagaaca
      241 aatcgaaaaa aattcaaaaa gcttatatca ggcactgtgg aattccgcag atatcctgcg
      301 ttcaaaaatg gacgctaatg aatacaagag ctatttacta ggcttggtct tttataaata
      361 cttatccgac aacatgctgc ggtatgtctc cgttctattg gaggaagaaa cggacgacct
      421 aaataaagca caagaccttt acgttgaagc atttaaagat gcagatataa aggatgatct
      481 cttgtatgaa ctaaaagatg agttttcata taccatcgca cccgcattga cctttaccgc
      541 gcaagtggca gccattcatg atggatcttt tcaattagaa gacctggtac aaggtttccg
      601 tgacattgaa caatccagcg aaaactttga gaacttattt gaagacattg atctgtattc
      661 taaaaaactg gggtcaaccc cacaaaaaca aaataaaacc atcgcagatg ttatgaaaga
      721 attagaaggg ttaaacatgg cggggcatgc tggcgatgtc ttaggcgatg cctatgagta
      781 cttaattggc caattcgcat ctgaatccgg taaaaaagcc ggtgaattct ataccccaca
      841 accggtcgca aaattgatga cacaaattgt tttacaagga aaagaagata aaaaaggctt
      901 ttctgtttac gacgcgacca tgggttcggg ctcgttactt ttgaacgcta aaaaatattc
      961 ccatcaaccg ggaaccattt cttatttcgg acaagagttg aatacgtcta cctataattt
     1021 ggcgcgtatg aacatgattt tgcatggggt tcccattgcc aaccaacact tgcataatgc
     1081 ggataccttg gatcaagact ggccaacaga agagccaacg aacttcgatg gggtgctcat
     1141 gaacccaccg tattctgcaa aatggtcggc tgataaaggc ttcttagatg acccacgctt
     1201 ctctgcctat ggcgtattag cccctaaatc aaaagcggac tttgctttct tattacatgg
     1261 ctactatcat ttgaaagata cgggcgtcat ggccattgtt ttgccacatg gggtgctctt
     1321 tagagggggc gcagaaggga agatacgcaa agccttgtta gaaaatggtg ccattgacac
     1381 cgtcattggc ttacctgcta acatcttctt taatacgtcc attccaacga ccgtaattat
     1441 cttgaagaaa gaccgtgcga aaaaagatgt gctctttatt gatgcgtcac aagattttga
     1501 aaaaataaaa acgcaaaata cgctacgaga ttaccatatt gacgctattt tagaggccta
     1561 taaaacccgt acaattaaag acaagtatgc ttatgtcgca gaatttgatg aaattgtgga
     1621 gaatgattac aacttaaaca ttccccgtta cgtggatacc tttgaagaag aagaa
//
LOCUS       DACEMS010000213         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEMS010000213 DACEMS010000000
VERSION     DACEMS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.222"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..505
                     /locus_tag="IW735_002689"
     CDS             131..505
                     /locus_tag="IW735_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1045642.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IW735_002690"
     CDS             complement(619..1248)
                     /locus_tag="IW735_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1045643.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IW735_002691"
     tRNA            complement(1382..1454)
                     /locus_tag="IW735_002691"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          284 g          556 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt aggggaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACEMS010000214         1594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEMS010000214 DACEMS010000000
VERSION     DACEMS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.223"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1485
                     /locus_tag="IW735_002692"
     CDS             <1..1485
                     /locus_tag="IW735_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1045644.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          632 a          251 c          307 g          404 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atca
//
LOCUS       DACEMS010000215         1578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEMS010000215 DACEMS010000000
VERSION     DACEMS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.224"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1297
                     /locus_tag="IW735_002693"
     CDS             128..1297
                     /locus_tag="IW735_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1045645.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          566 a          254 c          258 g          500 t
ORIGIN      
        1 atatcccata ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg
       61 gtagaaaaaa aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga
      121 ctttctcatg actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt
      181 agatgaatta tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct
      241 aactgctttt ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg
      301 taacgggaat tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc
      361 tagagatcgg aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga
      421 ttcattagaa acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc
      481 caaattaatt gaaaaaatgt atggtcacca ctatacgcca caaacaatct ccaacatgag
      541 taaattggtg gctgaagatg ttttagcttt taaagaaaga acgttagaag ccaattattc
      601 cgttatattt atggatgcga cgcatatccc tgtgaagcga cagactgttt cgaaggaagc
      661 agtgtatatt acgattggta ttcgtttgga tggaaccaaa gaagttcttg ggtttactat
      721 tgccccaact gagtctgctt atatttggaa agaagttctt caagatctta gaaaacgtgg
      781 gttagaagaa gttttattag tagtgacaga cggattaagc tgtattgaag aaagtatcca
      841 tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg catgtatcta gaaatatcgc
      901 tcataaagtt cgtgttcgag gtcgaaaaga aatttgtgag gatttcaaat tggtttacca
      961 agcgaattca aaagaagagg cattggatca catcgacttt atgactagga aatggaaaaa
     1021 gcagtatcca agagtcgtca atttactctt gaatcctgcc ctattaacct tttataattt
     1081 ccctcacgcc atcagacgaa caatttattc gacgaacctg attgaaggct ttaataagca
     1141 gctaaaacga tatactcgaa gaaaagaaca attccctaat gaagaatctc tagagagatt
     1201 cttcatttct caatttaatc aatataacca aaaattttta ggtagaattc acaagggatt
     1261 taaagaaatt caggatacat tagagtcgat gatttaactg taattaacag aatggatttt
     1321 ccatttacat ataattcttg acgctaccat aatctaaata accatcatat tcatctacta
     1381 cttatataat tgagttttat taaacttttt aagtttctat tctatcaaat atttaaacaa
     1441 tctctgcatc cgaaaagact acctcgtata gttttactaa caatattttt attagcgtgt
     1501 tcaaatcctg atttcttgta tcaattattt tctgttcact ttcaataaga aaaaagtctt
     1561 tacttccatg gataataa
//
LOCUS       DACEMS010000216         1519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEMS010000216 DACEMS010000000
VERSION     DACEMS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.225"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1469
                     /locus_tag="IW735_002694"
     CDS             <1..1469
                     /locus_tag="IW735_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1045646.1"
                     /translation="LTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEIS
                     VSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGR
                     TKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVA
                     ETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQ
                     EEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFT
                     GYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVL
                     EKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEP
                     IFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPV
                     KYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          509 a          273 c          317 g          420 t
ORIGIN      
        1 atcttacaac tgaaatccaa ctattgaatg aaaaggtcaa ttatttaacc aacaaattat
       61 ttggacgctc taaagagact ctctttgagg aaacgaatgg gcaattgaat ctttttagtg
      121 atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca gtaaaaggac
      181 atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata acagagaaag
      241 aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag atgaaagata
      301 tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg gattgtctca
      361 cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt tcttctttta
      421 aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat agtttagtgg
      481 cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa gagaactatt
      541 ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg catattaaag
      601 cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt aatcaagaag
      661 agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa acagatacct
      721 attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat tatcaccacg
      781 acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca gggtatttac
      841 actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc tattgtttcg
      901 cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac acggatattc
      961 ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag aaatggaaag
     1021 acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca ttatttattg
     1081 ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg gatgcagccg
     1141 ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat ggtcgtttag
     1201 aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga cggaaaaatt
     1261 ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg agtgtatata
     1321 aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta tttgataaga
     1381 taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct tggaacaagg
     1441 atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt ctattctata
     1501 ggtcgggtta tttgacgta
//
LOCUS       DACEMS010000217         1498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEMS010000217 DACEMS010000000
VERSION     DACEMS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.226"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..1438
                     /locus_tag="IW735_002695"
     CDS             50..1438
                     /locus_tag="IW735_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1045647.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
BASE COUNT          596 a          181 c          248 g          473 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttat
//
LOCUS       DACEMS010000218         1445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEMS010000218 DACEMS010000000
VERSION     DACEMS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.227"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..226
                     /locus_tag="IW735_002696"
     CDS             <1..226
                     /locus_tag="IW735_002696"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1045648.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IW735_002697"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IW735_002697"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IW735_002698"
     CDS             533..1336
                     /locus_tag="IW735_002698"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1045649.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          462 a          259 c          344 g          380 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggaca
//
LOCUS       DACEMS010000219         1404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEMS010000219 DACEMS010000000
VERSION     DACEMS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.228"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(20..427)
                     /locus_tag="IW735_002699"
     CDS             complement(20..427)
                     /locus_tag="IW735_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045650.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(446..1264)
                     /locus_tag="IW735_002700"
     CDS             complement(446..1264)
                     /locus_tag="IW735_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1045651.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          419 a          230 c          198 g          557 t
ORIGIN      
        1 aataccgcat gaaaaaaaat tattttggta atttttcaaa ctcttcttgt atttcttctc
       61 ttatgtgttc ttcttttgct actcctagtc tatgctggat atatgagtct gctaatgtat
      121 taacaatttt ttcgaaagaa attctccctt tttcactttg taactccgtc atatagtctt
      181 gtaaaattga gagtttgcta tttaatgccg gagagatctt tttaggaact gataccttgt
      241 ttcttttatc aggaattgaa actggtcggc ctcttttttt caatgtaata tttcttgtgt
      301 tactcaatgc actattgcta aagttatatt caccgcttat tttcacatct tggcgtttgt
      361 tattgggtaa taatggctca aattcaccat tctccatcat tttaggaata tcaaaatctt
      421 taggcattac ttatgcaact ccttattatt tatttaaaaa ctcttgtcta gcatctagtt
      481 ctgccaaaat atttataaat acttggtgtg ctttataatc ccattgatca aaatttccat
      541 ttttgtattt tcttaaatgt ataccaattt tagaatacct tctcaatctt ttttgataat
      601 gaatgagatt tttggatacc acggttccgt atttttcaat tgcttcttct aggatttctt
      661 catcaaaatc atcatttggt tctaccatac aaggtacaat atctataatt tgcaaatcaa
      721 gatcgtatct atcgaccatg aatttttgat aatttacata tttgttgact ccctctaagg
      781 attcttcagc tgtttgaaat gaaattatcg aatagtctga agctgccata gcattatcgg
      841 aaaattcgct aatggttggt ggtacatcaa taaatatttc ttgataattg ttctttattg
      901 gaagaagtaa ttttttcaag acagtaactt ggtctaattc attttcatgt ttattagcaa
      961 aagcggagaa tgatcgaaaa ctagtgtcac atgccagaag gtcaagattt tcatttacct
     1021 taataatact acttgctaat tctccatttg cgacaccatt tgttatagtg gttttgatat
     1081 cggttatacc gaaagttctt gctatgactt gtgttaagtt cgcttgaggg tctaaatcaa
     1141 taataagcgt ttttctattt cttatcattg cattgtcata agcaagcatg ctgactatct
     1201 tagttttacc tactcctcct ttaaaattgc caactgtaaa tgtagtccct tgaaaagtat
     1261 ccatgtaaat cctcccaatc tttaattgat aattaaagta taacttatat atcggaaatg
     1321 gtcaacatta aaagtataac ttaaataata atatctaaaa acgtttggat atagcggttt
     1381 ttaataataa aagtataagt tata
//
LOCUS       DACEMS010000220         1393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEMS010000220 DACEMS010000000
VERSION     DACEMS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.229"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1393
                     /locus_tag="IW735_002701"
     rRNA            <1..>1393
                     /locus_tag="IW735_002701"
                     /product="23S ribosomal RNA"
BASE COUNT          374 a          300 c          424 g          295 t
ORIGIN      
        1 aagtctgaag aggagtcaaa tgcttctttt cttaaggaca agctgtgatg gggagggaaa
       61 taatagtacc gaagttcctg atgtcacact gccgagaaaa gcttctagtg agaaaacagc
      121 ggcccgtacc gcaaaccgac acaggtagtc gaggagagaa tcctaaggtg agcgagagaa
      181 ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgatcat
      241 acgatcagcc gcagtgaata ggcccaagcg actgtttatc aaaaacacag gtctctgcaa
      301 aatcgtaaga tgaagtatag gggctgacgc ctgcccggtg ctggaaggtt aagaggagtg
      361 cttagcgcaa gcgaaggtac gaattgaagc cccagtaaac ggcggccgta actataacgg
      421 tcctaaggta gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaacgat
      481 ttgggcactg tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta
      541 cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt gagtgtctgt
      601 accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc gatggaggcg
      661 ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa tcgtggtggg
      721 agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt aacggaggcg
      781 cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc agaagggagc
      841 ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt
      901 ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct
      961 cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct
     1021 ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg
     1081 gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga aatttgagag
     1141 gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc agttgttctg
     1201 ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc atctaagtgt
     1261 gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc ctgagagatg
     1321 atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact gatactaatc
     1381 gatcgaggac tta
//
LOCUS       DACEMS010000221         1391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEMS010000221 DACEMS010000000
VERSION     DACEMS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.230"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(34..1053)
                     /locus_tag="IW735_002702"
     CDS             complement(34..1053)
                     /locus_tag="IW735_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1045652.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1064..1270)
                     /locus_tag="IW735_002703"
     CDS             complement(1064..1270)
                     /locus_tag="IW735_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1045653.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          382 a          298 c          233 g          478 t
ORIGIN      
        1 agttatgtaa tgctcctcaa cgaggagcta tttttatcgt ttaggaatat ttaaatacca
       61 acgtttgtca tggaaatctt gcgcaccgcc tttagtgttt ccctctggat cattcgttgc
      121 ccgcatcatg acatagactt tcttattagg gaagttacgc atgttaaaag atacatgata
      181 gccaacattt ccagaagtat tataagcttg atttacatct gatctataaa ttccatcagc
      241 tcttactcga gctaattctt tcccagtatt gtaatccata atgaagatat actcgtattt
      301 atagttagca atgtgccatc cagctacatg caaatttgca ttttcgattt ctccaaactg
      361 atcaatgtgg gcgtaattcg ttccatctgt cagtgtagga ttagctgcac cagctcgtgt
      421 tggatcaatg acaggcttgt tttcagaagt tgttggattt tcatcggtaa atccatgagc
      481 taaatcatat gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc
      541 tgtatgatcg ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt
      601 ataaggagtg tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc
      661 aacgtatgcc ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat
      721 ctgcacagga ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg
      781 accgacttga taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca
      841 tgaacgttgc atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg
      901 cagtaaaata tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa
      961 ttcattgttg atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag
     1021 gctcatcgca gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa
     1081 ttttcttata cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg
     1141 taataatcat caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga
     1201 caaaagtcgc taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc
     1261 tattgttcat actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg
     1321 ttttcttcta tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt
     1381 tcaattttat c
//
LOCUS       DACEMS010000222         1376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEMS010000222 DACEMS010000000
VERSION     DACEMS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.231"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..742
                     /locus_tag="IW735_002704"
     CDS             200..742
                     /locus_tag="IW735_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045654.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="IW735_002705"
     CDS             898..1347
                     /locus_tag="IW735_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ1045655.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
BASE COUNT          549 a          167 c          266 g          394 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaa
//
LOCUS       DACEMS010000223         1352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEMS010000223 DACEMS010000000
VERSION     DACEMS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.232"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            11..1350
                     /locus_tag="IW735_002706"
     CDS             join(11..452,452..1350)
                     /locus_tag="IW735_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1045656.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          565 a          175 c          235 g          377 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DACEMS010000224         1347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEMS010000224 DACEMS010000000
VERSION     DACEMS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.233"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            48..1343
                     /locus_tag="IW735_002707"
     CDS             48..1343
                     /locus_tag="IW735_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1045657.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          423 a          267 c          257 g          400 t
ORIGIN      
        1 aaatccttta gaattaattt ggctaccaat tcataggagg attcaagatg tctcatactc
       61 agcttatcaa agatacccta aatattctag acctaaatat ctatttcgag gaaaattgtt
      121 taactaaaga aaaatacaaa gggcaaattt gcttgattta caaaggaact cttctgtaca
      181 agccagaaga gtgttcccat tgtctttgtg tcgtcccctc taggatcatt cgttggggga
      241 cgacaaccgt tcggttgtta ctgaatgatg tgtctgagta tcgcacttat ctggagctga
      301 agaaacaacg atttaaatgt aagtcttgcc aaagaacatt cgtagcggat acctctgttg
      361 ctgaaaaaca ctgtttcatt agtcaaaaag ttcgttggtc tgtcattgct cgcttaaaag
      421 agaatacttc catgacggaa atcgctcgtc agaaaaatat ttccacttct tcggtctatc
      481 gtgtcatgaa gcgattctac cgcccattga atcctttcaa acagactttg ccaaaggtct
      541 tatgctttga tgaattcaaa tcggttcgaa gtgttgccag tgcaatgagt tttatcatga
      601 tggacggaca aagccatgca ttattggata ttgtcgaaaa tcgacgcttg ccttatttag
      661 aacgctattt ttctcggttt tcattagcga ctcgtgaagc cgttcaattg attgttattg
      721 atatgtatgc gccttatgtt tcgttggtga aaaagctttt cccgaatgct cagttgatca
      781 tcgatcgttt ccacatcgtc caacatattg gcagaacttt cttgaatcat cgagtgaaag
      841 aaaccacggt acgacttaaa ggaaattccc actctcaacg agggttaggg aaaaaattga
      901 agcgctattg gaaactccta caaaaagaag aagcaaagtt aaactatgaa aaacgggttt
      961 ggcgtgccag ttttaaagat tatttgactg aaagtgagat tgccgatcga ctgttagctg
     1021 cttgtcccaa cttacgccaa ggctatcagt tataccaaga cattctttat gcggtaaaga
     1081 aaagagatca gtcactattt gaggattgtt taactagaga cattagcggt ctgccagaaa
     1141 cctatacaac cactttacga acgtttaaaa agtttctgcc acagatccaa aatgcgcttc
     1201 actacagcta ttcaaacgga ccattggaat gcttaaacaa ccacatcaaa gtattaaaac
     1261 gcaatgcgta cggctttcgc aacttttaca atttcaaact acgaattatg attcgtcatg
     1321 gcaaaacatt tttaaccaaa taaacag
//
LOCUS       DACEMS010000225         1336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEMS010000225 DACEMS010000000
VERSION     DACEMS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.234"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(146..1129)
                     /locus_tag="IW735_002708"
     CDS             complement(146..1129)
                     /locus_tag="IW735_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1045658.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          377 a          285 c          254 g          420 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg
     1321 catttgcggt aataag
//
LOCUS       DACEMS010000226         1313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEMS010000226 DACEMS010000000
VERSION     DACEMS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.235"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(297..1244)
                     /locus_tag="IW735_002709"
     CDS             complement(297..1244)
                     /locus_tag="IW735_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ1045659.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
BASE COUNT          473 a          209 c          201 g          430 t
ORIGIN      
        1 aacccccttt ttatagcata aaaaagattt agaaggatat atgatcctat tgcatgagaa
       61 gtacaataaa attaataaga agtaacatta acgaaaaaac gtggaaatta ttcagtgaat
      121 acaaagaaat taaatcttga aaagtaatgg agtatggctt ggaaaagttc aaaaaatatc
      181 aagacaagct tctcaaaaaa agggattttg atatccaata aaaagagtga atcattgtac
      241 tgccctccaa aaaagtagta ccaaaaatct aaattttttg gaggatttta catgactcac
      301 tcttttttta tcaaagaaag tattttccaa gcagtttttt catccaaaaa taaatctgta
      361 aaaaaattac cattcaaggc gcctaaacaa ctctccgctt tgaacttact tttacaaata
      421 ccaattcgtt ttggaatatc gaaaatttct tttaaagaaa gaccaaaagt cttatcctca
      481 gatgctttga tgaattcacc attgatattg aagggtcttc cgtaaagaaa tccaacagta
      541 tccgtcatgc ttggaaatag gattttacga tgcttgttcc agggttcgac agaatcaata
      601 gaggataagg tgccaattcc agaaattaac atatctgcat gcgaagcggt tgctaaggta
      661 ttagctaaag aaggttctag agccattagt tcacgtgcag tttcattgat gacatataaa
      721 ggaatactga gagtcaaata agaagactca taacgttcag cagctttttg aactaatcgt
      781 gtggagccag ctaaggaatt atattttccg atttcgccaa tgaattgagt aaaaattaat
      841 tcttcacgag cacccatttt aaactgatca atcactttgt aaacggtatc tccccaagat
      901 agtgcaacca cattggcttt cttaattaat tcttgaatgt aatcggcagc aaattttgaa
      961 aataataaat cgtgttgtga taagtcttca ctacttttta atatatagat attcgtttta
     1021 aagtaatttt tgaagatttc ttctagctca taattcctag catacggaga attaattgaa
     1081 atactaacta attgtttttc acgtgcatct tcaatatatt ttaaagcttt atatctggtg
     1141 atcccatatt ttttagaaat ttcactaata ttgatatcct cgagataata gtcttcacaa
     1201 atttttacaa ggagtgattc atagtcagag aattccagat gcatagtata gctcctaact
     1261 ttttcaaata gatatttttt ctcattatac caaaaaaatt gaatttagtc ttt
//
LOCUS       DACEMS010000227         1280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEMS010000227 DACEMS010000000
VERSION     DACEMS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.236"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..1089)
                     /locus_tag="IW735_002710"
     CDS             complement(94..1089)
                     /locus_tag="IW735_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1045660.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
BASE COUNT          423 a          202 c          149 g          506 t
ORIGIN      
        1 tgaaaataca attaaatttt tttgaatagg taatatttta ataaaaaccg ggaaaatatt
       61 ccttgaaaaa aattttttta tattcttcat ctcttacctc acagattcta tcttaatcaa
      121 gaatttactt gctatcttac tacttgacat atagtaaggc aagtttttat ttattaataa
      181 aaactttact aattttttta attttggtga aaagtcggga tcaattttca cttctttttc
      241 aaagcgttgt aaaatattta tagaacgtaa ataatcttct ttattggtat agttattttt
      301 aatcattgtc atcatcatcc ttattgcttc atagacatag cgagttctaa cattaattga
      361 taattccgga taaaacttat ctatgaatat tagtatatct tcatggactt caaaagaatc
      421 aaaaattcta ggattgaagc ttcctctcag tatactttta gggctttggt aatagtgata
      481 tttagcagat gaattgacag caactttttc ggaatgatcg atcagcaaat aagtagtagc
      541 cttatcttcc attaatttac ccagtgggaa tcttatatta tcaaataaat ttctattgaa
      601 taatttatta tttgcagaat agccaatttt attatttata aataagtttt tcattgcttg
      661 ttctctatca aatatttccc attttctctc agcaattgta tgttctttta gttgttctaa
      721 acttgaaatc ttttttattt ttagcaccga gatatctgct ccataagttt gcgtgccaaa
      781 atatagtaac tcaagatagt ctggttctat ccaatcatcg ctgtcaataa agcacacaaa
      841 atctcctgtt gcagaatcaa gaccagaatt cctagccgca cttaaaccac cattcacttg
      901 atgtatcact tttaatcgca tatctttctt tttatattct tctgcaattt ccccacttcg
      961 atccggggaa ccatcattga ctactattat ttcaatattt gaatgtgtct ggttggaaat
     1021 agattctaag cagttttcta aatagtcttc tacgttatat gcgggtacta taacactaat
     1081 tttatgcact ctcttcactt cctaaaaatc atctttttta tttttacaaa tgtatcatta
     1141 ccagtaacaa ttttagtctt ccgaagttaa taaacataaa aaataacata tcgaaaaaaa
     1201 ctttattttt ttagaacttt tttatataaa ataaaataag ttttaggatt aattaagtat
     1261 aaaaataaag ctaatttttt
//
LOCUS       DACEMS010000228         1276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEMS010000228 DACEMS010000000
VERSION     DACEMS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.237"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            35..532
                     /locus_tag="IW735_002711"
     CDS             35..532
                     /locus_tag="IW735_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1045661.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="IW735_002712"
     CDS             complement(569..1252)
                     /locus_tag="IW735_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045662.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DACEMS010000229         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEMS010000229 DACEMS010000000
VERSION     DACEMS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.238"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            414..947
                     /locus_tag="IW735_002713"
     CDS             414..947
                     /locus_tag="IW735_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1045663.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACEMS010000230         1176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEMS010000230 DACEMS010000000
VERSION     DACEMS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.239"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..392
                     /locus_tag="IW735_002714"
     CDS             <1..392
                     /locus_tag="IW735_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1045664.1"
                     /translation="ASVNYLKKKNVQLIITSLPIMAEAIGNPLLDKFIKDLIIQILAM
                     IAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQRRLVYKNIVEDLKKGVA
                     IAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(416..766)
                     /locus_tag="IW735_002715"
     CDS             complement(416..766)
                     /locus_tag="IW735_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1045665.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(760..1023)
                     /locus_tag="IW735_002716"
     CDS             complement(760..1023)
                     /locus_tag="IW735_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ1045666.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          372 a          230 c          171 g          403 t
ORIGIN      
        1 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
       61 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      121 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      181 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      241 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      301 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      361 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      421 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      481 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      541 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      601 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
      661 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
      721 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
      781 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
      841 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
      901 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
      961 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1021 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1081 acttaattat ccttttctat taaattagat taacattcca tgaatccttg aacctctcat
     1141 gactaaagtc acgagattct tgggaataga gcatac
//
LOCUS       DACEMS010000231         1163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEMS010000231 DACEMS010000000
VERSION     DACEMS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.240"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            192..446
                     /locus_tag="IW735_002717"
     CDS             192..446
                     /locus_tag="IW735_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1045667.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="IW735_002718"
     CDS             447..764
                     /locus_tag="IW735_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1045668.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          411 a          176 c          232 g          344 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaagaca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atg
//
LOCUS       DACEMS010000232         1109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEMS010000232 DACEMS010000000
VERSION     DACEMS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.241"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..183)
                     /locus_tag="IW735_002719"
     CDS             complement(<1..183)
                     /locus_tag="IW735_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045669.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATP"
     gene            988..>1109
                     /locus_tag="IW735_002720"
     CDS             988..>1109
                     /locus_tag="IW735_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1045670.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT          342 a          237 c          145 g          385 t
ORIGIN      
        1 aggtgttgct gtttcttcta atagtgtact taattcctta atattttgtt ttccttcttc
       61 taacatccaa ctactcgctt tttcttcgcc ttcggtaaca aaaggcgcca ctcccttcgc
      121 ccatgttgct cgcccacata agacaccatt ctccataaag gacaacgcgg agataccacc
      181 catctaaata acatccataa acctttctag tagaagaata gtgatagtat ccatcccttt
      241 ttctactggg aatacaatta cacgattcaa agcttgatac acaacatttc ccttcttata
      301 aaccgctatt gtaaccgaat tgttttgaat cacctttttg ggatagaaac tggctccttt
      361 ccgcaacttc ttaaatgttg gtttctttta tactttcttt gtagcaacta caaatttctt
      421 caaatctcta aatgtttgca taacaaccgc ctttctgact tttactcagg atggtattta
      481 aaaatcagca aaataaggct tctctccatc ttttttattt cctagctgct ttaaagacac
      541 tcttgctgtt ttttaatctc tatcaagggg gctctgaatc tattttgatg aacttatctt
      601 attaatttct caaatgagct gtcagacaca actgtatttt ttcaatctca taccggacaa
      661 ctatgccagc ttaaaaaaat ttcctctggc aaaacttctt tattgaaaaa tgacaatgaa
      721 aaaagaccat tctttttgtt gaatgctcat tgtgtaaaca tatttcctgc gactgctctt
      781 cgaaccattc tcagtgcaat ccctaattta tctattaaat cttcttttag ttcctcatct
      841 ttcatattca aaatttcctt tgtatgttac acaagtttgt tctctaaata acgcgaattg
      901 taaaattaag ttagaaaaat aaaaaggcat ttatggtaca ctcaaattgt atttccgacg
      961 aaagaaaaca aaggagtgta accataaatg acctacaaac atcttaccat agacgaactg
     1021 acaatgatag aatcatatta tcttcaacat aataagccgg ttgaaatcgc taaccgaatg
     1081 ggacgtgcta tacaaactat ttataatgt
//
LOCUS       DACEMS010000233         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEMS010000233 DACEMS010000000
VERSION     DACEMS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.242"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..980
                     /locus_tag="IW735_002721"
     CDS             6..980
                     /locus_tag="IW735_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1045671.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACEMS010000234         1082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEMS010000234 DACEMS010000000
VERSION     DACEMS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.243"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            700..>1082
                     /locus_tag="IW735_002722"
     CDS             700..>1082
                     /locus_tag="IW735_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1045672.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT          413 a          161 c          244 g          264 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 ga
//
LOCUS       DACEMS010000235         1044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.244, whole genome shotgun sequence.
ACCESSION   DACEMS010000235 DACEMS010000000
VERSION     DACEMS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.244"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..342
                     /locus_tag="IW735_002723"
     CDS             91..342
                     /locus_tag="IW735_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045673.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            324..596
                     /locus_tag="IW735_002724"
     CDS             324..596
                     /locus_tag="IW735_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045674.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            763..>1044
                     /gene="tnpA"
                     /locus_tag="IW735_002725"
     CDS             763..>1044
                     /gene="tnpA"
                     /locus_tag="IW735_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1045675.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHP"
BASE COUNT          387 a          150 c          199 g          308 t
ORIGIN      
        1 ttcctataca atgagacacg tgttccaaaa aaacattata aggaaagatt gtcggtagct
       61 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ttatcgaaca
      121 ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa
      181 gtaggcgtca aaccaagcac aatcaaacct tctgaatacc aagctttaga actaacgtat
      241 gcgaaattta tcgaaacgaa aaaagctaag actgtttcaa gtgaatggtt agattatttt
      301 gaaaatatca ataagtatgg cgcatgttac atcatgaagt aatcagacgg attcaaaaag
      361 actttagttg tatggaagaa atgaagtact ttgttcaaaa tgttgaagaa cagttatatt
      421 tggaaagcga aacgagtcaa acaaaggtca cagtcagcca taatcgttta gatgtgtata
      481 aacgtcgtcc tgaatcatta aaaaaaggag cggagcttct acgactagaa acagaaaaat
      541 acgtcatgaa tgtttaccta gaaaaaagtg agttggtcga aaattttatg gactagccga
      601 gaacacccgt gattttaatc atgggatgaa tcggattcag ttgtgaccta gctttagcta
      661 ggttaaacaa ctgacaaata taaactaaat aatagtgaaa tgtcttgcaa taattgattt
      721 tgtagaaata catatattaa aataattgcg aggtgttttt gtatgtcaga aattcattat
      781 ggacggggtt atgtctattc tattcaatat catcttgttt ggtgtgtaaa atatcgacat
      841 gatgttttgt atggtcaggt agatattgat gtcaaacagt ttttgaaaca aattgctgat
      901 gataataata taaaaattat agaaatagaa tcagataaag accatattca tttactcatt
      961 gaatgcaccc cacaacacta tattcctagt attgttaaag tatttaaagg cgcatccgct
     1021 agactgctgt ttaaaaaaca tcct
//
LOCUS       DACEMS010000236         1020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEMS010000236 DACEMS010000000
VERSION     DACEMS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.245"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            73..>1020
                     /locus_tag="IW735_002726"
     CDS             73..>1020
                     /locus_tag="IW735_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1045676.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLS"
BASE COUNT          353 a          175 c          189 g          303 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataatattcc tagaaaatca
      961 ctggattatc gaacaccttt ggaagtattt ttgagttacg taagtattga tgatctgtct
//
LOCUS       DACEMS010000237         1013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEMS010000237 DACEMS010000000
VERSION     DACEMS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.246"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(62..280)
                     /locus_tag="IW735_002727"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IW735_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IW735_002728"
     CDS             complement(546..740)
                     /locus_tag="IW735_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045677.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          348 a          175 c          152 g          338 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aag
//
LOCUS       DACEMS010000238          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEMS010000238 DACEMS010000000
VERSION     DACEMS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.247"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>994)
                     /locus_tag="IW735_002729"
     rRNA            complement(<1..>994)
                     /locus_tag="IW735_002729"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACEMS010000239          991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEMS010000239 DACEMS010000000
VERSION     DACEMS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.248"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(21..94)
                     /locus_tag="IW735_002730"
     tRNA            complement(21..94)
                     /locus_tag="IW735_002730"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IW735_002731"
     tRNA            complement(108..181)
                     /locus_tag="IW735_002731"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IW735_002732"
     tRNA            complement(209..281)
                     /locus_tag="IW735_002732"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IW735_002733"
     tRNA            complement(301..374)
                     /locus_tag="IW735_002733"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="IW735_002734"
     tRNA            complement(384..457)
                     /locus_tag="IW735_002734"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="IW735_002735"
     tRNA            complement(468..553)
                     /locus_tag="IW735_002735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="IW735_002736"
     tRNA            complement(570..641)
                     /locus_tag="IW735_002736"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="IW735_002737"
     tRNA            complement(653..725)
                     /locus_tag="IW735_002737"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="IW735_002738"
     tRNA            complement(743..824)
                     /locus_tag="IW735_002738"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="IW735_002739"
     tRNA            complement(832..904)
                     /locus_tag="IW735_002739"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="IW735_002740"
     tRNA            complement(908..980)
                     /locus_tag="IW735_002740"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DACEMS010000240          973 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEMS010000240 DACEMS010000000
VERSION     DACEMS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.249"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..630
                     /locus_tag="IW735_002741"
     CDS             <1..630
                     /locus_tag="IW735_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1045678.1"
                     /translation="PNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWR
                     LELQLRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARH
                     TKYKYRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          307 a          155 c          234 g          277 t
ORIGIN      
        1 ccaaataaaa ttagccaatt tttggcgggt tcgatgaaga actttatcca tgatttattt
       61 ttagaaccac atttctcacg tgctgatatt gcttgcgaca ttatcgatgt gcctgatgat
      121 tttattacgc aatatcgtgt ggttgaccct gtttcattca aaccaatcta tgggcggtct
      181 gggaagctgg aaacggcata ttggggtagt cgtgccagtg agcgacaaat aaggatgtac
      241 aacaaaaaat tggaacaaga aaccaaacgg aaaattgtac caccggaaat aaaaacttgg
      301 tggcgactgg aattgcagtt acggcgtgga aaagctactg attggtatgc gatggttcat
      361 gaaagtttag acagtttcgc aagtcctcat tttttgccga ctgatacaaa gcatactgat
      421 agaattatga ttgcaggatt gatttcagat caaaatttat gggctggctt ggctcgtcat
      481 acaaaatata aatatcgaaa tttgttgaaa aaagaaagcc aaagtgatga gttagttaat
      541 catttacgag agacattttc ggaatcggca gatgttttga aaaaagaatt ggatacgtgg
      601 cttttggggt tggacgtgac ggaagaatga gtggatgaat ttttgtccac ttttttattt
      661 tcagcacaag tcggcatggt gggtcgtgcg tttgcagcaa gagcgaaaaa acgctaacga
      721 atccgcaaat gccgacttgg ataaaacagt tgcgcaaaga gacttggctt tttataataa
      781 atttagggat atcccagtgt cactctttgg ggtaattcgg gggaaggagt tacccctcac
      841 tctggaaaat tagagcgaag cagacgagat tatcccaatg aatatgacgg cttactcgga
      901 gtgataccga gtaaaaaagt cagataagtg agagtaaatt tacgtcaatg tttaagataa
      961 gcgattattt tgc
//
LOCUS       DACEMS010000241          942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEMS010000241 DACEMS010000000
VERSION     DACEMS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.252"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            307..921
                     /locus_tag="IW735_002742"
     CDS             307..921
                     /locus_tag="IW735_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1045679.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
BASE COUNT          345 a          158 c          169 g          270 t
ORIGIN      
        1 cacctcgaaa tcaaaaataa gaaaacgttt tcttaataaa tcttaacaga taaatcaagt
       61 tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa attttcttga
      121 ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacaca tactatttcc
      181 tccatcatta gtttttgtct atataaggtt taccctaaat agacactggt ataacattga
      241 aaaatatggc ttcctcgtgt atgattaggg tgtattttaa atagttgcag aaagtaggta
      301 aaagttttga gagttgcata catacgtgtc tcatcgatcg atcaaaatga acaacgccag
      361 attgaagaaa tgaaaaaatt cggtgcagag agaattttca tcgagaaaca atctggcgca
      421 acgattactc atcgacctgt tttccaagaa gcattagatt acgtccgaga tcaagatatt
      481 tttattgtgg aagctattga tcgacttgga agaaattatg acgagatcat cacttcagtc
      541 aattacttga agaagaaaaa tgtgaagctg atcattacta gtttaccgat catggctgaa
      601 gcaattggca atcctctcct ggataaattc attaaggatc tgatcatcca aattttggcc
      661 atgatcgctg aacaagaacg gactgaatcg aaacgtcgcc aggcacaagg aattaagatc
      721 gcaaaagcaa acggcgtcta caaagggcgg cccaaattat acagtgcaga cgcaaaagat
      781 ccccaacgtc gccttgttta caaaagcatc gttgaagatc ttaaaaatgg tgttgcgatt
      841 gctaagattg ctaaagacta caatgttacg agacaaaccg tgtatagaat aaaaaaagac
      901 agcatggtca atgacaaata gttgatcatg ctgcttttat ta
//
LOCUS       DACEMS010000242          908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.253, whole genome shotgun sequence.
ACCESSION   DACEMS010000242 DACEMS010000000
VERSION     DACEMS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.253"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..>908
                     /locus_tag="IW735_002743"
     CDS             82..>908
                     /locus_tag="IW735_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1045680.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQK"
BASE COUNT          303 a          168 c          180 g          257 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaag
//
LOCUS       DACEMS010000243          887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.254, whole genome shotgun sequence.
ACCESSION   DACEMS010000243 DACEMS010000000
VERSION     DACEMS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.254"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            40..756
                     /locus_tag="IW735_002744"
     CDS             40..756
                     /locus_tag="IW735_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1045681.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          307 a          167 c          173 g          240 t
ORIGIN      
        1 gcttttttat ttgtctcgct taattataca aacggcgaat tgaaaaaaag gattttagta
       61 acaattggtc tagctttttt gttagctggt tgtaccaacg ggaatgaatc gaaaaaagaa
      121 tcaacaaaag aaacagcgaa agcaacagtt gcaactacta caacaactag cacagagagc
      181 aagtcaactg cagcaagtac agaaacagca gatacctcta cttcgtcaga gtcttccaaa
      241 acgactgaaa caactcaaac atctacttca agttcttctg tagaggcttc atcttcctct
      301 tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg cagacaaatc cgcacaatta
      361 gaggcattta tgaccaaatg gggccaaaca atgggacaac aatacaaaag ttatacgaat
      421 caaatgagtg tggacttata tggtcttaaa gtccctcaag ctattttgaa cggggagtgg
      481 aagatggcta ttggtggagt gcctgtttct gcagaatggt cagaaagtgg gacaggtcaa
      541 gcagactatc aaatcacagc cgtctattcc gatgcagaga cagaaccata tctgaaaaag
      601 cacgtctatc tattcgggtt tcagcaaaat cagccaaaag tattggtgac acaacaaaat
      661 caggggaatc ccgataatta tctttacttc aacgagacag cgaataatga attgaaaaat
      721 ggctttaatc aaatcgttta tggacaagca aattaatgag ataaacaagg ggttgtgaca
      781 tgtcttttat cacaacccct tgctcagttt atttgttagt cttttatttt ttggctctat
      841 actttttgat gttggtgaac ttcaccaaca tcttttttgt ataaaaa
//
LOCUS       DACEMS010000244          859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.255, whole genome shotgun sequence.
ACCESSION   DACEMS010000244 DACEMS010000000
VERSION     DACEMS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.255"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            108..>859
                     /locus_tag="IW735_002745"
     CDS             108..>859
                     /locus_tag="IW735_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1045682.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLET"
BASE COUNT          370 a           95 c          141 g          253 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaa
//
LOCUS       DACEMS010000245          817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.256, whole genome shotgun sequence.
ACCESSION   DACEMS010000245 DACEMS010000000
VERSION     DACEMS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.256"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>817
                     /locus_tag="IW735_002746"
     CDS             <1..>817
                     /locus_tag="IW735_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1045683.1"
                     /translation="LFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTI
                     LNELETYNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKS
                     TSSRNAEIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKR
                     HLKDTETDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIH
                     KAKHKAEELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLI"
BASE COUNT          340 a          133 c          137 g          207 t
ORIGIN      
        1 tgctttttaa aaatgaattt cgtagagcct tacatgaaaa atggatcgac gatgatggtc
       61 tattatattt ggaatttaca caagcagacc taatggacaa attaaattgc taccaaggca
      121 aagtaaaaac cattttaaac gagttagaaa catataattt gatttcagtc gtaaaaggag
      181 aatttaaccc aaaaacgaaa aaaaatgaaa aaaataaata ctatttactt caacctgaaa
      241 taagtgccga tgatcttttt attcaaaatt ctttcgatga cgatttaagc gaaactattg
      301 ataaatcaac ctcttctcgg aatgcggaaa tcgcacgtcg agaggaaact gctaaaaccc
      361 ttgataacac tggaaatgcg gaaatcgcac gtcgagaaaa aacagctgaa acccttgata
      421 acactggaaa tgcggaaatc gcacaagatt attatattaa taaatcttta gacactaaaa
      481 gacacttaaa agacactgaa acagaccaat tacaaaatca agttttgcta gataacttcg
      541 ttgaaatcat gaaagaagac agcataaaca catttgtacc agaaaaggta ttaagcttga
      601 taaaaacatt ttctaacact tacgaagaag cccaacaaac cgtcaccaca atacacaaag
      661 caaaacataa agccgaggaa cttgagaaga cggtagttgt gtatgaagaa cttgaatctt
      721 acggaattga tgcggataaa ggattatata tgacgttgct gaaagcttat caaaaacaaa
      781 agactgagaa agtaaataat ttgcagaatt tgatttt
//
LOCUS       DACEMS010000246          788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.257, whole genome shotgun sequence.
ACCESSION   DACEMS010000246 DACEMS010000000
VERSION     DACEMS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.257"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..779
                     /locus_tag="IW735_002747"
     CDS             <1..779
                     /locus_tag="IW735_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1045684.1"
                     /translation="MLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGV
                     SLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGEL
                     ISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLI
                     KNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGIP
                     LKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          338 a          107 c          101 g          242 t
ORIGIN      
        1 atatgctaga ttactactat tttgaaaaaa atttatcata taattatgta atacatttaa
       61 aaacttttac aggaatgatt tttaaattcg ccaaccgtcg atatggatta aaatataatc
      121 ccgtaaatgg ggtttcttta gcaagaaaac ctaagacaaa cgaagaaata aaaaaagaaa
      181 aagatggtta tttatcaaaa gaagaagttc aaatgattgc tagtaaacaa aaaagttcac
      241 atcaacagtc acgttattct ttaattacta aatttttgtt tttaactgga ctacgttatg
      301 gtgaattgat atctttgact gaatctgatt atgatggtaa taaaattacg gtttctggta
      361 cttatgatta cgagttaaaa attaaaacaa caactaaaaa tacaggttct tatagaacca
      421 ttgaattatc aagtaatgct aaagaaatca tagatcaatt aattgaagaa aacaaattaa
      481 ttaagaataa taaagataaa tacattttca tctctaaaaa tggtaacccg atatctattc
      541 aagcatataa tcaatcttta agagcagtat ccaaagaatt aaacatagat aaaaaagttt
      601 cttctcacat gttaaggcat tcccacattt ctctacttac agaattagga atcccattaa
      661 aagccattat ggatcgtgta ggacatgaag attcaaaaac aacattaaag atttatactc
      721 atacaactaa aaatatgcaa aatcaactag tagaaaaact aggaaaaata gaaatttaaa
      781 ttgcccct
//
LOCUS       DACEMS010000247          788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.258, whole genome shotgun sequence.
ACCESSION   DACEMS010000247 DACEMS010000000
VERSION     DACEMS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.258"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..76
                     /locus_tag="IW735_002748"
     CDS             <1..76
                     /locus_tag="IW735_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1045685.1"
                     /translation="YFVATVSENTEEQIRKYIQNQKKK"
     gene            88..>788
                     /locus_tag="IW735_002749"
     CDS             88..>788
                     /locus_tag="IW735_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1045686.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNL"
BASE COUNT          311 a          118 c          125 g          234 t
ORIGIN      
        1 ctactttgta gctactgtta gcgaaaatac agaagaacaa attagaaaat atatacaaaa
       61 tcagaaaaag aaatgaggtg aatccgtatg ctacaacata gggcttataa atttagaatc
      121 tatcctaatc aagaacaaga aatattaatg actaaaacta ttggctgttc aagatttgtc
      181 tataattatt ttttgaattt gtggaatcat gaatatacga ctactggaaa aggattatct
      241 tataattctt gttctgccat gcttccacaa atgaaaagaa atgcagagac aatttggtta
      301 aaagaagttg acagtattgc gattcaatcc tcattgaaaa atctttctga tgctttttct
      361 cgcttcttta aaaagcaaaa taaacgacca caatttaaaa gtaaaaaaca tcctatacaa
      421 agttatacaa ctaaaaatgt gaataatagt atcgaaatta gaaacaattt cgtaaaacta
      481 cctaagttag gatttgttaa atttgcgaaa agtcaagagc ccaaagggcg aatcttaaat
      541 gctacgattc gaaaaaattc gagtggtaaa ttttttgttt ccatcctgtg tgaagaagaa
      601 atagttgagt ttcctaaatc taattccgct attggaatcg accttggtat aactgatttt
      661 gcaatccttt ctgatggtca aaagattgat aacaataaat tcacgtcaaa aatggcaaaa
      721 aaactcaaac gtgaacaacg taagttgtca aaacgtgcat tactagctaa aaagaaaggc
      781 gtcaatct
//
LOCUS       DACEMS010000248          774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.259, whole genome shotgun sequence.
ACCESSION   DACEMS010000248 DACEMS010000000
VERSION     DACEMS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.259"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(248..772)
                     /locus_tag="IW735_002750"
     CDS             complement(248..772)
                     /locus_tag="IW735_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045687.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          319 t
ORIGIN      
        1 gacgctccct taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat
       61 tagatatgaa aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag
      121 atgagagtca ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg
      181 atataggaag tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat
      241 ttttttgtta tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta
      301 tatcttctaa attagaagat atttgtatat caatatggaa tatattacct tttttgtatt
      361 tttttatgtt atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag
      421 ctggatttag tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt
      481 tttttatttt tttacctttg taaaaatatt cgattgaata ttctatttgg cacgactcac
      541 ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca aaatctaaac
      601 tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa tttatctttg
      661 gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct ctatcaaaat
      721 agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc aaac
//
LOCUS       DACEMS010000249          764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.260, whole genome shotgun sequence.
ACCESSION   DACEMS010000249 DACEMS010000000
VERSION     DACEMS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.260"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..561
                     /locus_tag="IW735_002751"
     CDS             <1..561
                     /locus_tag="IW735_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HAQ1045688.1"
                     /translation="WNELLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCC
                     VHVSRNIAHKVRVKDRKEICDDFKAVYQASSKEEANTFLGSMIEKWQKTYPKVTQSLI
                     KNQDLLTFYEFPPGIRRSIYSTNLIESFNKQIKKYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT          270 a          131 c          129 g          234 t
ORIGIN      
        1 tggaatgagt tgttacagga catttactcc agaggggttc atgatgtctt actgtttatt
       61 accgatggct taaaaggcat gaaagatacg attcaccaga tttatcctaa agctaaatac
      121 caacattgct gtgttcacgt ttcccgtaat attgctcata aagtacgtgt caaagatcga
      181 aaagaaatct gtgatgattt taaggctgtt tatcaagcta gctcaaagga agaggcaaat
      241 acctttttag ggagtatgat tgagaagtgg cagaaaactt atcctaaagt gacgcagtca
      301 ctgataaaaa atcaagattt attgactttt tatgagtttc cgcctggtat ccgccgaagc
      361 atttactcaa ctaatttaat cgaatctttc aataaacaaa tcaagaaata cagccgcaga
      421 aaagagcagt ttcaaaatga agaatcacta gagcgtttcc tagtatccat ctttgataca
      481 tacaatcaaa aattcttaaa tagaagccat aaaggcttcc aacaagtgac ggatacatta
      541 gcttcaatgt ttactgagta acccattatt ttgcagaagg acgagttatt tacacaaaat
      601 tattgacgct cccttttata agcaatttag ttaacataat gtaataaggt agaaatgttt
      661 aaatactgaa aataaattac aattgtatta ccaaactagt ttacgctgct tttgattaca
      721 aattagctaa taatatgcgc taatttatat atgtaccgcc cctc
//
LOCUS       DACEMS010000250          741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.261, whole genome shotgun sequence.
ACCESSION   DACEMS010000250 DACEMS010000000
VERSION     DACEMS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.261"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>741
                     /locus_tag="IW735_002752"
     rRNA            <1..>741
                     /locus_tag="IW735_002752"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          151 c          220 g          144 t
ORIGIN      
        1 caaagagggt aaaaaccccg tagacgaaat gtggaagaca cctaggagga tcctgagtac
       61 ggcggaacac gagaaattcc gtcggaatcc gggaggacca tctcccaagg ctaaatactc
      121 cctagtgacc gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccggaagggg
      181 agtgaaatag aacctgaaac cgtgtgccta caacaagtca aagcccgtta atgggtgatg
      241 gcgtgccttt tgtagaatga accggcgagt tacgattgca tgcgaggtta agttgaagag
      301 acggagccgc agcgaaagcg agtctgaata gggcgtttga gtatgtagtc gtagacccga
      361 aaccatgtga tctacccatg tccaggttga aggtgcggta aaacgcactg gaggaccgaa
      421 cccacgtacg ttgaaaagtg cggggatgag gtgtgggtag cggagaaatt ccaaacgaac
      481 ttggagatag ctggttctct ccgaaatagc tttagggcta gcctcggaat tgagaatgat
      541 ggaggtagag cactgtttgg actaggggcc catctcgggt taccgaattc agataaactc
      601 cgaatgccat tcattcatat ccgggagtca gactgtgagt gataagatcc atagtcgaaa
      661 gggaaacagc ccagaccacc agctaaggtc ccaaaatata tgttaagtgg aaaaggatgt
      721 ggggttgcac agacaactag g
//
LOCUS       DACEMS010000251          736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.262, whole genome shotgun sequence.
ACCESSION   DACEMS010000251 DACEMS010000000
VERSION     DACEMS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.262"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            311..709
                     /gene="spxA"
                     /locus_tag="IW735_002753"
     CDS             311..709
                     /gene="spxA"
                     /locus_tag="IW735_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1045689.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          266 a          105 c          134 g          231 t
ORIGIN      
        1 aacataaaaa atcttaagaa atcggtttca tttagctttt gagataaaat aatagtacag
       61 ttttatacgc tgtttcatat gtttagaaaa tttgaattag atttgaaaac tctaattttt
      121 aaatgataaa aaaagcgaga atgctggttt tactgtatat taaccttaga taactttcat
      181 ttttttaaaa aaattatgaa aaaaaggtag ctatttaaaa gttgattatg tataatagaa
      241 acagatggga gattattatg atcttctgtt acaaaaaagt gattcttatc acgaaaagga
      301 gtggtgcacg ttgttaacat tatatacttc cccaagttgt acttcctgca gaaaggctcg
      361 tgcgtggttg caagaacatc agattccatt tgttgaacgt aatatttttt cagaaccatt
      421 gaacagttct gaattaaaag cgattttaca aatgacagaa gacggaacag aagaaatcat
      481 ctcgacacgt tccaaagtat tccaaaaact gaacatggat ttagatgatc tgccgttaca
      541 agaattattg gaacttgtgc aaaacaatcc tggattgctt cgccgtccaa tcatgattga
      601 tgataaacgt ctgcaagttg gttttaatga agatgaaatt cgtcggttcc taccgcgcga
      661 tgtacgacaa ttagaattac gtcaagcaca attgatggct ggattataga aggataatac
      721 gaaacttgga aaagac
//
LOCUS       DACEMS010000252          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.263, whole genome shotgun sequence.
ACCESSION   DACEMS010000252 DACEMS010000000
VERSION     DACEMS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.263"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW735_002754"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW735_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1045690.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="IW735_002755"
     CDS             complement(481..675)
                     /locus_tag="IW735_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045691.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DACEMS010000253          704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.264, whole genome shotgun sequence.
ACCESSION   DACEMS010000253 DACEMS010000000
VERSION     DACEMS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.264"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            69..>704
                     /locus_tag="IW735_002756"
     CDS             69..>704
                     /locus_tag="IW735_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1045692.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGY"
BASE COUNT          290 a           61 c          130 g          223 t
ORIGIN      
        1 acactagata ttatagagcc ataatatgct gtataatatc tagtgtatag gcttaagtgg
       61 aggtatatat gaaaatatta actgttgaag atgataatat gataagagag ggaataagtg
      121 aatatctttc agaatttgga tatactgtta ttcaagctaa agatggaaga gaagccttgt
      181 caaaatttaa tagcgatata aatttggtta tcttagacat tcagatacct tttataaatg
      241 gtttagaagt gttgaaagaa attagaaaga aaagcaattt accaattcta atcttgactg
      301 catttagtga tgaagaatat aaaattgatg catttactaa tttagtagac ggatatgtag
      361 aaaagccatt ttcattgccg gtcttaaaag ctagaataga ttctttaatt aagaagaatt
      421 atggacattt agagaagttt gaatataagg atctatcggt taattttaat agctatacag
      481 ctaaaataaa tggtgaagaa atagatgtaa atgcaaaaga actagaagta ttaaaatgtt
      541 tattggataa taatggacaa gtgttaacaa gaatgcaaat tattgattat gtatggaaag
      601 atagcgaaga aactccttat gatcgagtaa tagatgtata tataaaagaa cttagaaaaa
      661 aattacaatt agactgtata actactataa gaaatgtagg atat
//
LOCUS       DACEMS010000254          702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.266, whole genome shotgun sequence.
ACCESSION   DACEMS010000254 DACEMS010000000
VERSION     DACEMS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.266"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(62..388)
                     /locus_tag="IW735_002757"
     CDS             complement(62..388)
                     /locus_tag="IW735_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045693.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
BASE COUNT          202 a          162 c          107 g          231 t
ORIGIN      
        1 tttttagagg ctgtgacaaa gattttgtca cagcctcttt ttccatataa acggcgttcg
       61 cctaccagct gcctggtaaa tctgtttcct cttcgttaag aagcgaaaat ccgtcaaatg
      121 tttcgccatc ataataacga agcagctcat ccatggacaa gttcatcgtt ttttcttttt
      181 cttcttccgc tgattcagcc agtagctgtt cccgatacac ctgatacata ccagctattt
      241 tttgctgcat cgcttttcta gtttcacctt ccgcattgat tattggcaat tctttgatat
      301 agccacgata aatcaaccga tcatctgaaa attcaatggg taattcaacg agtgtcaacc
      361 cgacttcctg aaaatattct tccagcatct attatccccc cattcttttt cttaaatgaa
      421 ttttagctta cctactgaaa attttccagt ttcattattt tgagaaaaaa agggaatgtg
      481 acaaaaatcg tgtcacatcc cctttttccg aataaatggt gttcttctgc caactcctca
      541 atcataagtc acaatattca acaaacatga gaaactgttt tttgaaattg ttccttgtga
      601 ttcagagtta aacgacagtc tcacaacctc ttggtaaacg gcgttcaaaa gcttgcatct
      661 gcggtaataa gcccaaccaa acgaaaaata tgagaagcta tt
//
LOCUS       DACEMS010000255          690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.267, whole genome shotgun sequence.
ACCESSION   DACEMS010000255 DACEMS010000000
VERSION     DACEMS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.267"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..573
                     /locus_tag="IW735_002758"
     CDS             <1..573
                     /locus_tag="IW735_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1045694.1"
                     /translation="IAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKEL
                     DKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVN
                     QSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRAL
                     VLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          252 a          133 c          119 g          186 t
ORIGIN      
        1 atcgctaaag caattcaacg tgcaattacc atgagttacc gcctaggtgc tcttgcacaa
       61 gaatcaatta atgttgtttt aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat
      121 atcaaagaat tagataaagc catcgaacaa gtaattgttg tcattccaga atatcaatgc
      181 ttaaccagta ttccaggcgt tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt
      241 caaatcgaaa gatttgaaga tcagacaaaa ttggcaaaat acgctggatt aagttggaaa
      301 gtgaaccaat ctggaaacta tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga
      361 tattttagat actacttagt tgaagctgcc aactctgtaa aaaattatct tcctgaatac
      421 aaagcttttt atcaaagtaa atataaggag gttccaaagc atcaacacaa acgtgcactc
      481 gtcctaaccg caagaaaatt tacgcgtctg gtggatacgc tactacgtaa acaccaactc
      541 tatacgccac caaggagtgt gatagagaaa taacaatccg ttattgacgc aaatccttga
      601 atcaacccga aaaaaatttg attttgatcg ggtctagttt cgtgcgcttc aaaacatatt
      661 actcaataaa gaaaatctta aatttcatct
//
LOCUS       DACEMS010000256          658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.268, whole genome shotgun sequence.
ACCESSION   DACEMS010000256 DACEMS010000000
VERSION     DACEMS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.268"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>658
                     /locus_tag="IW735_002759"
     CDS             <1..>658
                     /locus_tag="IW735_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1045695.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKL
                     R"
BASE COUNT          219 a          135 c          116 g          188 t
ORIGIN      
        1 caactgcctt ttttaaaacg ttattttagt catttttcac gagaaattcg tgaagcggtc
       61 gaatggatcg tgattgatat gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca
      121 aaagcacagc tgattattga ccggtttcac atcgtccaac atattggtcg tacctttcgc
      181 aatcatcgaa tcaaagaaac gaaccaacta ttaaaaagca aagaacaaaa acactatcaa
      241 ttaggtaagc aattaaaaag atactggaaa cttcttcaaa aagatgaacg caagcttgat
      301 tatactcgac gcctttggcg acctggcttc aaagctcatt taacagagac cgatattgtg
      361 gatcgcttac tgaaagggag ccctgctttg cgtgtaggct atcagttata tcaagacttt
      421 ctttacgctg taaaggaacg agactacgta tcgtttgaag aactacttac caataatatc
      481 atgcttcccg aaggctatca gacgaccttg agaacgttcc agaaatttct gccacaaatc
      541 aaaaatgctt tacaacaaag ttattcaaat ggcccacttg aatgtctgaa caaccatatc
      601 aaagtgttaa aacgaaatgc atatggcttt cgtagctttt ataatttcaa attgcgca
//
LOCUS       DACEMS010000257          602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.269, whole genome shotgun sequence.
ACCESSION   DACEMS010000257 DACEMS010000000
VERSION     DACEMS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.269"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(318..581)
                     /locus_tag="IW735_002760"
     CDS             complement(318..581)
                     /locus_tag="IW735_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ1045696.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          226 a          104 c           79 g          193 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tctatttttt
      601 ta
//
LOCUS       DACEMS010000258          599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.270, whole genome shotgun sequence.
ACCESSION   DACEMS010000258 DACEMS010000000
VERSION     DACEMS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.270"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            46..>599
                     /locus_tag="IW735_002761"
     CDS             46..>599
                     /locus_tag="IW735_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HAQ1045697.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIM"
BASE COUNT          185 a          122 c          113 g          179 t
ORIGIN      
        1 cctttagaat taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac
       61 cttatcaaag aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc
      121 acaaaagaaa aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca
      181 gcccaagaat gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca
      241 acaaccgtcc ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa
      301 aagcaacgct tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg
      361 aaaaaacatt gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa
      421 aatacctcaa tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt
      481 attatgaagc gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt
      541 tgttttgatg aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgat
//
LOCUS       DACEMS010000259          587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.271, whole genome shotgun sequence.
ACCESSION   DACEMS010000259 DACEMS010000000
VERSION     DACEMS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.271"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            26..412
                     /locus_tag="IW735_002762"
     CDS             26..412
                     /locus_tag="IW735_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1045698.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT          221 a           93 c          118 g          155 t
ORIGIN      
        1 ataaaaataa aaaaggagat ctttcatgaa aaaaatttta gggttgatcg ttatcatatt
       61 gattgctttt ataggatgga aaggttggga ctactatcag tctacttatg taggtaaaga
      121 ttattatgcg gtagtcaaag ctccaatgcc tgcagaaact gatatcaaag cagataatgg
      181 ggaagtaatt ggaagaggtt ttaaatacaa cgtagatgct tatgctgaaa acggtgaaaa
      241 aagacagtta gatttcgatg tcattacttc tggtgactct gccaatggtt ctgcttaccc
      301 cgaaggaacg atcttgcagt tgaaagcaag cgaaaagcgg atcatagaga aaaaagtaat
      361 taccagcgat aaggtaccct catctgtaaa agaaaaatta ggcctatctt aagctaagaa
      421 tatatcgcaa aaaagcactc gaagtgagtg cttttttgct tactgattct agaaaacaaa
      481 aaaacagaat taaccttcga agtcagcctc actgttctta aagtcctaaa acaaactaaa
      541 gagcaaggca atcaatcgat ttctatacaa acaagggact gtgacaa
//
LOCUS       DACEMS010000260          565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.272, whole genome shotgun sequence.
ACCESSION   DACEMS010000260 DACEMS010000000
VERSION     DACEMS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.272"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>565
                     /locus_tag="IW735_002763"
     CDS             <1..>565
                     /locus_tag="IW735_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1045699.1"
                     /translation="FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQ
                     MIQENIKEELGLIATVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEM
                     TDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAI
                     PPEPTKEKSYGNSQVLPRDYTRRNEIEL"
BASE COUNT          199 a           95 c          131 g          140 t
ORIGIN      
        1 ttcagaagat atgtggctga tgaagatcta ctgatttact cgatcgatga atcaatctta
       61 aaagtgaccc gatcactgaa tctttttacg actgaaggaa cacgaagtca acggagaaag
      121 aagctggctc aaatgatcca ggagaatatt aaagaagagc taggattgat tgctacagta
      181 ggtgtcggag ataatcccct tttggctaaa ctagccttag acaacgaagc aaagcataat
      241 gaaggatttg ttgcggaatg gacgtacgaa aacgttcctg aaaaagtatg gaatattcct
      301 gaaatgacgg acttttgggg aattggctct cgaatgaaaa aaagactcaa tcagatgggg
      361 attatgagca ttagagatct tgctaactgg aatccttata cgattaagaa tagactaggt
      421 gtgattggct tacagctgta cttccatgct aacggaatcg atcgaacaga tatagcaatc
      481 ccgccagaac cgacaaagga aaaatcttat ggtaattcac aagtgttacc acgagattat
      541 actagaagaa atgaaatcga gctgg
//
LOCUS       DACEMS010000261          552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.273, whole genome shotgun sequence.
ACCESSION   DACEMS010000261 DACEMS010000000
VERSION     DACEMS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.273"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(279..458)
                     /locus_tag="IW735_002764"
     CDS             complement(279..458)
                     /locus_tag="IW735_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045700.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
BASE COUNT          176 a          107 c           87 g          182 t
ORIGIN      
        1 attctacgtt gtcgggttat ccagcttgca accgttcctt gtgggtaggc atataactca
       61 caaaattgtt ctacagtgat tcctaaattc ttgataatgt aaacattgat cgggtgcgag
      121 taaacaaatg tattgataga catggtgcgt aagcctactt tcttttatta ttgcgtttgc
      181 aataatagtt taacacacgc attattctag tatctactcc tatttgtaaa aacaaaaaag
      241 gattcataga gataactcta tagaatccgt aaaatacctt atatatataa cgggaagata
      301 taaaacctaa aattatgata acctgctgca gcttctacat agaaagagaa gcgctcaaca
      361 ccaacctcat ttatcaaacg ttggccataa ccccaaatct cttctgatag ctgatcaaag
      421 gttatatact ttagctcata ttgctggata atgttcattc taatcgactc catttattat
      481 cctggtaaaa ttcgatatga cgtatttcgt cgtagtgaat tttgtctttt ccaacgtaga
      541 tccccagctc at
//
LOCUS       DACEMS010000262          549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.274, whole genome shotgun sequence.
ACCESSION   DACEMS010000262 DACEMS010000000
VERSION     DACEMS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.274"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            205..>549
                     /locus_tag="IW735_002765"
     CDS             205..>549
                     /locus_tag="IW735_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045701.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          245 a           70 c           81 g          153 t
ORIGIN      
        1 aaaagaattt ttcaaagttt cactagacga aatagagaaa gttgttttag aaaattataa
       61 tggaacagta acatttacaa aattagctaa agctgaacaa tatagacgat ctttagaatt
      121 gtcaaaagat taaatatata aaaagccttc gggctttttc ttttaacaat taataggaac
      181 gtatattcga aaggaataga aataatgtgg ttagaaaaaa taaatgacaa aaaatttaag
      241 tacaccgaac gttatacgga tcccttaact gaaaaaaaga gaaaagtgtc cgttacttta
      301 tctagcaatt cacgccaagc ttggaatcaa gctaacttac ttttgaatga aaaaatcgca
      361 gaaaaaataa aacgaaatga agaattacca ctaacttttg gagaattaaa aactaaatgg
      421 gatgaaaaat acaagccaac tgtaaaagaa agttcctatc gaacaactca ggtttatcta
      481 tcattaattt caaaatatat aaaagatgat gtacttgtaa aaaacgttaa tagtaatctt
      541 atacaagat
//
LOCUS       DACEMS010000263          527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.276, whole genome shotgun sequence.
ACCESSION   DACEMS010000263 DACEMS010000000
VERSION     DACEMS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.276"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            258..527
                     /locus_tag="IW735_002766"
                     /pseudo
     CDS             258..527
                     /locus_tag="IW735_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          181 a           94 c           76 g          176 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataac
//
LOCUS       DACEMS010000264          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.278, whole genome shotgun sequence.
ACCESSION   DACEMS010000264 DACEMS010000000
VERSION     DACEMS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.278"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..132)
                     /locus_tag="IW735_002767"
     CDS             complement(<1..132)
                     /locus_tag="IW735_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1045702.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACEMS010000265          504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.279, whole genome shotgun sequence.
ACCESSION   DACEMS010000265 DACEMS010000000
VERSION     DACEMS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.279"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          165 a           97 c           76 g          166 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat agataaagtc cgtataattg tgtaaaagta
      481 aaaaggccat ataacagtcc tttt
//
LOCUS       DACEMS010000266          496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.280, whole genome shotgun sequence.
ACCESSION   DACEMS010000266 DACEMS010000000
VERSION     DACEMS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.280"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>496
                     /locus_tag="IW735_002768"
     CDS             <1..>496
                     /locus_tag="IW735_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ1045703.1"
                     /translation="YGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADG
                     LTLDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTE
                     HRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFS
                     VCTEI"
BASE COUNT          181 a           90 c           95 g          130 t
ORIGIN      
        1 atatggcaaa ctactctatc aaatttggaa aaagaaaaat aaaaaatcct tttattcatg
       61 gaaaatggat gaaacgtaca tcaaaattaa aggaaaatgg cattatttgt atcgagccat
      121 cgatgcagat ggtttaacct tggatatttg gttacgtaaa aaacgggaca cacaagcagc
      181 ctatgctttt cttaagcggt tagtgaagca gtttgatgaa ccgaaggttg tagtcacaga
      241 taaagccccc tctattacaa gtgcctttaa gaaactaaaa gaatacggct tttatcaagg
      301 gacagaacat cgtaccatta aatacctgaa taatttgatt gaacaagacc atcgtccagt
      361 aaagagacgc aataaattct atcgaagttt acgcactgcc tctaccacga ttaaaggcat
      421 ggaagccatt cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc
      481 ggtctgtact gaaatc
//
LOCUS       DACEMS010000267          489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.denovo.281, whole genome shotgun sequence.
ACCESSION   DACEMS010000267 DACEMS010000000
VERSION     DACEMS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.denovo.281"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..414)
                     /locus_tag="IW735_002769"
     CDS             complement(<1..414)
                     /locus_tag="IW735_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045704.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          186 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacata
//
LOCUS       DACEMS010000268        16867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.053, whole genome shotgun sequence.
ACCESSION   DACEMS010000268 DACEMS010000000
VERSION     DACEMS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.053"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            458..1321
                     /locus_tag="IW735_002770"
     CDS             458..1321
                     /locus_tag="IW735_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1045705.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1421..1903
                     /locus_tag="IW735_002771"
     CDS             1421..1903
                     /locus_tag="IW735_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1045706.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2166..2633)
                     /locus_tag="IW735_002772"
     CDS             complement(2166..2633)
                     /locus_tag="IW735_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1045707.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            2756..3607
                     /locus_tag="IW735_002773"
     CDS             2756..3607
                     /locus_tag="IW735_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1045708.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(3858..5816)
                     /locus_tag="IW735_002774"
     CDS             complement(3858..5816)
                     /locus_tag="IW735_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1045709.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5813..6685)
                     /locus_tag="IW735_002775"
     CDS             complement(5813..6685)
                     /locus_tag="IW735_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1045710.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(7027..7899)
                     /locus_tag="IW735_002776"
     CDS             complement(7027..7899)
                     /locus_tag="IW735_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1045711.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            8027..8596
                     /locus_tag="IW735_002777"
     CDS             8027..8596
                     /locus_tag="IW735_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1045712.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8663..9400
                     /locus_tag="IW735_002778"
     CDS             8663..9400
                     /locus_tag="IW735_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1045713.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            9631..10899
                     /locus_tag="IW735_002779"
     CDS             9631..10899
                     /locus_tag="IW735_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1045714.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            11386..11850
                     /locus_tag="IW735_002780"
     CDS             11386..11850
                     /locus_tag="IW735_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1045715.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            11834..12463
                     /locus_tag="IW735_002781"
     CDS             11834..12463
                     /locus_tag="IW735_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1045716.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            12817..14295
                     /gene="msr"
                     /locus_tag="IW735_002782"
     CDS             12817..14295
                     /gene="msr"
                     /locus_tag="IW735_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1045717.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKVQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            14342..14788
                     /locus_tag="IW735_002783"
     CDS             14342..14788
                     /locus_tag="IW735_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1045718.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(14987..15502)
                     /locus_tag="IW735_002784"
     CDS             complement(14987..15502)
                     /locus_tag="IW735_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1045719.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            15619..16821
                     /locus_tag="IW735_002785"
     CDS             15619..16821
                     /locus_tag="IW735_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1045720.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         5339 a         2990 c         3497 g         5041 t
ORIGIN      
        1 gtgagaaaaa gtaaaagaat agcgtgtttt aaagatggaa ctttttttgt tttttattta
       61 tctgacaaaa atgaagtgat tttgttagaa ataaataatt gttccaattg ataaaagagg
      121 tatgatgtat cgcaaagaaa aatccaagca gcgttctgat cagcagtttg aacaacaatt
      181 tgctttgtac agtaggtagt aagcggatta actaagggaa taaaagatat ttttaccgct
      241 ttgtttactt agaatctgtc ttccccaaaa tttatttttc taaaggtaaa acatagatag
      301 gcagataata ccaattatcc gttacgaaac gctgcttgag aattttcttt agatccatac
      361 aacattttct acatctgact aaattggttc ctgattcatc aagtaattga ctttctttga
      421 taatatgata aaaaggcaat caaggaggaa gataaatatg cttaaagatc tgaaccaatt
      481 gatcgattac atcgaagaac atttgacaga aggactttcc tataaaataa tagccgaaaa
      541 tacaggaatc tctgaatatc aattaaaacg catctttacg tttattgcag ggatgtcgct
      601 aatggactat atcaaaaacc gtagattggc tttggctaat cgcgatcttc tagatggatc
      661 aagagtgaca gaaacggcgt ttagatatgg atatgaaaca gtcgaaggtt tttcaagagc
      721 ttttcgtgat tggtcaggat atctgccttc agaaatcagt agatacggca tacaaaaatc
      781 ttttccacgc ttgacatttt ttattgatgt cagaggagga aaatcgatgg aatttcgtat
      841 cgaaaagaaa gaacagtttt atgtggcagg tgtggcgaaa aaagttcctg tcaaatttga
      901 aggagtcaat caagaaatac aaaagttagc gcagacgatc acggagactc aaagggaaga
      961 gatgcataag ttaggagatt tatatccaaa tcaagtaatc aacgtttctt atggatttga
     1021 tggagaccgc ttggaagaaa agggcagttt gatgcacatg attggctttg ctacgacaaa
     1081 agaaaatccg tacgctgatt tagaaatgct tgctatagaa gaacacactt gggccatttt
     1141 tcctaataaa gggccatttc cccaaacttt acaagatact tgggggaaaa tctatgcaga
     1201 atggcttcct tcttcagatt atgggttagt cgaagctccc gagatctctt ttacaaaatg
     1261 gggagaggat ttcagtaatg tttacagtga gatttggatt gcagtgaaag caaaaaaata
     1321 gttaaaaaat aaaaatatcc ctagaaaaaa gagcgtaatc gtgtcatttc cctttaattt
     1381 tatgtaagat gaacttaata taatagaaag ggtgggagaa atggcaaagt ataggatcga
     1441 agaaaaacca gattttacgg tgacgggtat tggtgtggaa ttgaagagta gcaatacgga
     1501 ttttgtcggt ttggctgcag agaaacaagc atttgagcaa aaagttatcg gtaatggaac
     1561 aatagatgca ttgaaacaag ctgctgaaaa tcattatttc tttacagtca acgaacccta
     1621 taacgggaaa atgatgtact atgtcggtgt tcaaacgagt caggatgttc caaacactac
     1681 gcgaatcatc ccattcccaa aaggaacgta tcttgttgta agcgctttag cagaatcgcc
     1741 tgaagaatta agaaatgatt tgacgaatac ggcctttggg catgttttaa gagaagtaga
     1801 agattacgcc tatgttgggg gtccaaatgc tgtagtcgaa atgggagtta ccaacgggaa
     1861 atatatggga gaaatctgga taccggtagt caataaacca tgataatata gaggtcgtga
     1921 aagtagcgct ttgacctgag cattagagag gaaaaatggg aaatagcttt ccatattttg
     1981 catttttcag aactaatgcc gagggtcagc tacttgaaca ccgtttatca agacgtcatg
     2041 acttcagcgc tttgacctga gcattagaga ggaaaaaggg gcatgacaag gcttttgtcc
     2101 cccggagaat aaaagaattt atgtagacga ttccgatcag aaaaatgcag gcacagtaga
     2161 gagaattata aaaatctctc tacgaggcct gtgttttctg gtcttttttc tttttccaaa
     2221 acggctattt tatgtatcag actatcaagt gtgatcttag ccatttcttc ttctgccttt
     2281 ttctgcagcc gagcgtagct ttcttctaaa gcttcttgga tattcgcacc aacgacgcaa
     2341 tttggatttg tttttggatc gacttggata agctgtgtat taccttcgat gctgcgatag
     2401 acatctagta aagaaatctg atctggactt ttggcaaggg aaggctgcgg ttttccggtt
     2461 tttgtaaaga tcaaaccgga ttttttgagc tgactcatga ttttacgtac atttgctgca
     2521 tttgtttcga tactactggc aatcatctca ctagaaagtg atcgggtatc ttgaaacatt
     2581 tcaatatagg caagtacatg gattgcatca ctaaattgca gagaatactt catcagttgt
     2641 catcctttca ttttttttgc ttgacttatc ttaccacact ctttatgata aaggtgtaat
     2701 ttataaatgt acagctgtaa atcttaacaa ataaaaagaa tagggagtgg caaatatgca
     2761 ttatttagta acaggagcaa ctggaggttt tggtggttat gcgctggaat tcttaaaaaa
     2821 gatggttcca caaaatgaaa tctatgtact agtacgtagt gaagaaaaag gagaagcact
     2881 gaaagaagct ggatttaata tacgtattgg tgattatgat gatttagact caatgaaaga
     2941 agcgttgaaa ggaatagatc gattgctttt tgtctctggc gttccaggaa atcgtcaagc
     3001 cgaacataag aatgtagtga aggcagcaca agcagctggg gtatcttata tcgcttatac
     3061 aagttttgca gatgccgatc actcgatcag tatattagcg ccagatcacc aattcactga
     3121 aaaaatgatt aaggattcag gtatttccca tacattttta cggaataatt ggtatgtaga
     3181 gaatgaatta cctatcattg ggcaggcttt aaaaactggt caattcgttt atgctgcagg
     3241 aaatggaaaa acaggttggg cattgaaacg tgaatatgca gaagctgcag caaaagtact
     3301 agtagaaagt gactctccag aaatcttaga attatccggc aatctaactg actatgaaga
     3361 attagccaag gcattagagc gtgcgacagg taaagagtta gaaattattg aagcaagtga
     3421 tgcagcgttt gtcgaaaatc taaaagaagc cggattccct caagaagcag ctgacatgtt
     3481 tttagcgatc caacatgata ttaaaaagga tcagttagct atttcctctg atgatttaga
     3541 aaaagtattg ggaaaaccat taacttctct tgaagcagca ttaaaagaaa tattggatca
     3601 ggaataatgt ataaagaggt tgtgaaaaag ccgattagtt ttgagtatga agaaaaattt
     3661 tgtgaaaaag ctgttctgca tcaagtaata agaagagtca aataaaaaat agctcttcat
     3721 atgtttcgtt tagctggact tattaccgcg gagttggcag gagaacgccg tttttattcg
     3781 gaaaaaggag ctgagacaag atttttgtct cagctcctgt ttgtttatcg ataaaaaagt
     3841 ctggggaaga cgatggctca ggctttgttg gaaacagagc cgattttatc atttgctttt
     3901 acatcacgtg cgggttcgat ttctaagctt tggactttgt cgccatttgt gaatacaacg
     3961 accatatccg tgtttttgcc tgcagattga atagcggcaa gatcaactaa agcaatcaat
     4021 tgacctcttg cgactttttg accttcttct acatataaag tgaatggttc gccttttaag
     4081 tcaacagtgt tgatgcccat atgaagcaag acttcgatac cagcatctgt ttggattccg
     4141 acagcatgtt tcgttgggaa aatgtttgtg atcgtaccag cgattggaga gaagatttct
     4201 ccattttctg gtaatacagc atagccgtca cccatcattt tttccgcgaa tacatcatct
     4261 gaaacttcag tgattggaat cagtttccca tttgcgatag cgtaaatatc ttgttcgatc
     4321 gtttcaccgg caaatgtttc tgttttaacc acggtgtttg tttgtgcagg tcgtgctgca
     4381 gcaccaccat gtagttttgc catctcatct gcaacgaatt gtacttctgt acctacgatg
     4441 acttgcgcat tttttccgtc gatgatcttc ataccaggta cgccagtggc tttgatttta
     4501 ttttgatcga tcaggcctgt atctttgact tgcaaacgaa ggcgtgtagt acagttatcg
     4561 atcgaagtta cattgtcggc tccgcctaat gcagcgtaga ttttgcttgc ttgtgccgca
     4621 tatttattgt ctgaattagt cgttgcagta tcgtcatctg aaagatctgc ttcctcgcgg
     4681 ccaggagtca tcagatggaa ttttttgata gcaaagttaa agccaaagta gtaaatcact
     4741 gctgttacta atccttggac gatcaacatc aatggctgat tcgcaatcgg atttttcaaa
     4801 gataggaagt aatcgacaaa tccggcacta aaggcaaatc ctgctgtcca atggaagaat
     4861 gcactcactg ctaaagaaat cccagtgaaa attgcatgca agacatataa tggccaagca
     4921 acgaacataa atgaaaattc aagtggttct gtcacaccag taaagaatga agcaaaagct
     4981 gctgctagca tcaaggaagc tgtttttgct ttgttttctg gacgtgcatt tctgtaaata
     5041 gcatatgctc ctgctggaag cccgaacatc atgattggga aaaatccagc ttgatacatt
     5101 cctgttacgc cttttgttcc ttcagaagcc aaaaatttac caatatcatt gattcctgct
     5161 acgtcaaacc agaacacgga gttcattgct tgatgcaatc ctgtagggat cagtaaacgg
     5221 ttgaagaagc cataaagacc tgcaccaccc cagcctaaac tcaaaatgaa tttagcaaaa
     5281 gaaactaaag cagagtaaac cggaggccat acgagcagta gcactgcact gatcagcaac
     5341 atgacaaagg cagccaagat tggcactaga cgcttgccgc taaagaaaga taaagccatc
     5401 ggtaattttg cttcgtggaa acgattataa agagctgccg caatcaatcc ggcaataata
     5461 ccaataaata cgttactgat ctgagcaaaa gatggatcaa ctgattctgg tttgatattc
     5521 aataaagtag ctactgaatc agtttttaaa acatttactg gaacttcaaa tgcgaccaaa
     5581 cctgccaagg cagcagcacc tgatttgtcc actgacatcc ctagtgctaa cccaacggca
     5641 aacagcaaag gtaattgacc taaaactgcg ttaccgccag cttgtaaaaa atgagcgaca
     5701 atgtcgttac tataagatgc ccaccagttt ccgatcccta ctagtaaagc agcagcaggt
     5761 aagaccgcaa ctggaagttg aagggaacgt cccattcgtt gtagatatgc tttcaatttt
     5821 tttcctcctc atgattgtta acatttgttt gtacgattgc gcttcttaaa cgctcgatat
     5881 ggatggccaa gtaacccagt tcttctttcg tgatcgcgag ctggtattct ttggtcagca
     5941 acacacggat ttttttagaa atcttatagc tttctgggta ttttcgtttg accatttcta
     6001 cgatttccgt gtctaaaaca gcatattgtt gcgcatgggt tcgttgtaga agcagtcgta
     6061 aatgattcac cagtcttgaa taattcagtg aaaatggttc tgaatggata tctgtttcca
     6121 attctgattc cactaaatgg atgatgtcag atacaatcgt cacttcacgg atactgcgat
     6181 ggttattgtt ccgaccgctt cgtccgctgt gaatatggat tgcgatatat cctgcttcgt
     6241 cataagtata tggaatccca agctcttttg tcagatactc tactgcccat tgagcaatac
     6301 tgaattcatc actgtaaagc acttcgattt ctcttaaaag tttgttgcgg atgacgatac
     6361 cgttattaat gttctccgca gaaaaagcaa tgtggtcggt caaagcgatc aatacatgtt
     6421 cattcaactt ttccatcaaa acagtttccg catgatcaat gatcgtttct gctgcaaata
     6481 aaaagcgttc atcaatctga ttaagtaaat tttgcaactt gatttgacct tctggttcca
     6541 tgacaaatat ccgttcgaca tcacgatcaa aaatcaaatc atttcttttt ctgtcaaatc
     6601 caatcccttt tccaatagct actttttcct gtccaccgtc atctaccaat actgcatttt
     6661 gattaaagat ctttttgatt ttcatgcgcg cacccttttt gaagatttgg ttttcacgtt
     6721 tgctagacca gttgattgcg gccaaacaaa aatagacacg aaaaacctcc tagggaaacc
     6781 atgttcctca gagatttttc atgcctagta tgatctagtc acatgaaacg aattattgtt
     6841 aacgtaatca attttatata gacagttatg ttttgtcaat tcttttttta taattatagt
     6901 aatttactgg aattatcaga aataaaagga agaaataaat taagaaactt atgttattta
     6961 atcgttggat cttttgctta tcttattcgt tgttaaaaag ctgaaagcga aggaaaaaaa
     7021 ctctacttaa ctgtatttct ccgttaggac atctaaaaat ttacgagcag caggagaaag
     7081 aggctggtct tttttccaaa tgagattcag tgaagcttgt aatggcggat cgagtgggat
     7141 gaatattttt tccgtcccat aagtattgat gatcccatct agagctaatg caccaaaagt
     7201 cccgttatcg accatcaaag aagcattgta caataagtta tatgttccga cgatcgataa
     7261 ctgatctatg ctgaaaccta gccattcagc tagttgactg tccactgacg tttgccggga
     7321 aatgatcaaa ggaacagttt tcagttcctg cggatggatg acggtttgct ttgctaaagg
     7381 atgatttcga tgtatcaaaa gtccccaacg atcttgggct ggcagtcgaa atgactggta
     7441 tttttgtttg tttgtcggtt cgattacgag tccaaaatca agtaatccgc tatccagttt
     7501 gtctatgata tcatctgcat tcccgctata taaatgaaac tggatatcag gatgcgcctt
     7561 gatcaacgaa tgaaggctat cagaaatgaa agagattgct ttagtttcgc ctccgccaat
     7621 gtagatttct ccagagatga ttgcgtcatt tgttaaattg ttcgccgttt tttctaccaa
     7681 cgacaagatt tcttttcctt ttgaataaag atatcgacca gaatccgtca actgtatctc
     7741 tcgggagccc cgagtgaaaa gtgtcacacc aagttcttct tctaaatctt tcagttgttt
     7801 tgagagagta ggttgcgtga tatgcagaga ttttgctgct cggctgatat ttttttctct
     7861 agctactgct aaaaaatacg tcagtatacg aatatccatt ttttccctcc tgtttttaaa
     7921 aaagcatacc actaagtata gttaaaagga atagttaagt atgttttata agtattagac
     7981 atgataagcc gtccgttata aagtaaggat aaaaggaggg agcaaaatga caagcctcat
     8041 cgaaaaacaa atcgtcaata aagagattcg agtaacggat ccgctctttg aagtgatcca
     8101 tcagatccaa gctgaaaacg aagagccttt agcagcgctg aatactgggt accatgaacc
     8161 agcagatatc cgcaaacggc tagaaaatat cattagtgac aaagtagatg atacggtgac
     8221 tgttctgctg cctttttata cagatttcgg caaacatatt agtataggga aaaatgtctt
     8281 tatcaatcgt caagtgatgt tcgttgactt aggtggcatc tgtttagaag attcagtatt
     8341 gattggtcct cgtgtcaacc tcatcacggt gaaccatcta accgatccaa aagaacgaag
     8401 aggcttgtca gtaaagccaa tccacatcaa gaaaaatgct tggatcggag caggagcaac
     8461 tattctgcca ggcgtaacga ttggcgagaa tgccattgtt gcagcgaatg caactgttac
     8521 gaaagatgtc ccagacaata ccatcgttgc aggaatccct gccaaaatcg tcaaacctgt
     8581 cgaacggatt atttgaacag taagatgaaa taagagatag tgagtataag caagagattt
     8641 ttcaaaacag gaggagaata gaatgaatga tttatcagaa aaagtgattg tcatcatggg
     8701 cgcttcaagc ggaatcggtg aagcaacaac gaagctatta gcagaaaaag gggctaaact
     8761 agttatagct gcacgccgag aagatcgcct aaaagcaata aaagaatctt taccagaagc
     8821 tgagctatat attcaaacag cagatgtaag agattttgca caagtccaag cagttatcga
     8881 tttagcgatg gaaaagttcg gtcgaatcga tgtattgtac aataatgcag gaatcatgcc
     8941 aaccgctcca ttagttgaag gtcaccgtga tgaatggcaa aatatgcttg atatcaacat
     9001 catgggtgtg ttaaatggaa tttccgccgt acttccaatc atggaaaaac aaaaatctgg
     9061 gcatatcatt tcaactgatt ctgtggcggg acatgttgtt tatcctgatt ccgccgttta
     9121 ttgcggaaca aaatttgctg tccgtgcaat catggaagga ttacgtcaag agcaacggca
     9181 aaataacatc aaatctacga tcatctctcc aggagctgtt caaactgaat tatatcaaac
     9241 catcagcgac aaaaaggctg ctcttgaact tcatgaagcg caaaaagaat ggggattgac
     9301 ctccgaagat atagcttctg ctgtagcatt tgcgatagaa acgccagacc ggatgtctgt
     9361 cagtaacatg attatccgtc cgacgacaca ggaagtataa tccataacgg ataatatcca
     9421 aagagaatta cttgttaaat agttattcaa acgaccatca atttaaggcg tgaatggaga
     9481 agaatattct tcatttacgt ctttttatgt agaaaaagca aatttatcaa aaagaaacaa
     9541 atgttattta aatatggaca ttgacttgct tttacttttt tagttacact gttatttagg
     9601 aaaagtttca taattcaaag gagaaaaaag atgaaagcaa agaatttatt atttagcgtt
     9661 attcttttag gaaatctagc agttgccaac acaacagtgc tagctgaaga atcgacagaa
     9721 actgtcgcaa ctcagacatc gagttcttca gaaacaggaa ttgaaaacga tacagagagt
     9781 actttgcagt cagctactgt ctcagaaaat acaatttcaa ccgaagaacc aacaactgaa
     9841 acaacagaac aaacacaaac cgaagacagt acatcagcag aagctcagaa caatccagag
     9901 aatgcagaag aaatcacgaa ccaattgaca gatgggcaaa cattgacaca tacccatcaa
     9961 gcagatttgt ctgaattcga aaactatact ggctatgctt tttatgctcg ctcatctgct
    10021 aattctcaac aagcgttcat tgatagtatt gcctcaactg cacaaagttt ggcaagtgcc
    10081 aatgacttat atgcatcagt catgattgct caagccatcg ttgaaagtgg ctgggggaac
    10141 agtactttgg cttctgcccc taattacaat ctatttggga ttaaaggaag ctataatggc
    10201 caatctgtta cgatgcctac ttcggaatat gtgaatgggc aatggattac ggtgaatgcc
    10261 gcttttagaa aatatccttc gtacaaagaa tcattgcaag ataacgtaac ggtattgaag
    10321 acgacttctt ttcagccagg cgtttactac tattccggtg catggaaaag caatacgaac
    10381 tcttacaaag atgctacggc ttggctaaca ggtcgttatg ccactgcgcc taattatggt
    10441 tctacattga acaatgtaat cgaaacctat aatctgacac agtatgatac agcaccaact
    10501 tcaacttctc cgatgtatcg tctgtataac cgtcatacag gagaacattt atatacattg
    10561 aatgccggag aaaaaaatta tctgccaact gtaggctggg aatatgaagg gattgcttgg
    10621 caagcaccaa acagcggaca acctgtctac cgtttataca atccttacag tggagaccat
    10681 cattacacaa tggcacaatc tgagatcaac tctttaacaa aaatcggttg gcgttatgaa
    10741 ggtctaagtt tttattctgg tggttccaag ccaatttatc gactatttaa tccaaatgaa
    10801 aaaactggta cccatcacta taccttgagt gctaaggaaa gagattggct aacaccgatg
    10861 ggctggagat atgaaggaat aggattttat ggttattaaa aagaggtagt gaaaaagctg
    10921 ccctgcatca agtagtaaga acagccaaac aaaaacagat tttcatgttt tttgtttggt
    10981 tgaacttatt accgcagatg caagtttttg aacaccgttt actcaaaaaa ggttgtgaca
    11041 acatcttgtc ataacttttt tattactgtt taagcatact gatccaattt ctaatattcg
    11101 acgggattct tgcaaaaggc gatttcggaa atttttggcg gtgtaaaatt ttctactgca
    11161 gaagagctat cttctatagt ttgataagaa tcaataatct tggttttagc gttgtgcttt
    11221 ctttatcatt ttttccgttc attctacaaa aaatttcttt tctctatact tttctattga
    11281 gagcgttttt tttattacac agctgattct ttgacttttt tcttttttta ttctattatt
    11341 gacaagtcaa tcgttgacga gtcaattatt agaaaggagg aaattatgtc tttagaagag
    11401 attagtcacc ttttatatca aatcaaatta gccgatcaaa aaatcgtcca gatctttgaa
    11461 gaaaatatcg gtataagtct tactagatac gagatcatga tgagactgaa agaagaaaat
    11521 ccattgctac aaagcagttt gcaagaaaag ttgcagattg atcaaggagc agttactcgt
    11581 catctgaagg tattggaagc aaatggctat gtttcgcgtc agagaaatcc tcagaacaac
    11641 cgggaagtgt atgtagcgct aacagaaaaa gcccagaaga aaatcgagca ttgtgaaatg
    11701 gaccataaag atacaggttc cgtgctatcc ccggctttca gcgatgtaga aatgaaacaa
    11761 tttctaacac ttttagataa attgaatacc caactttgca atataacaac agaaacgaaa
    11821 gaggttaata ataatggaac aaaaaaatga tttttcaaca attatgactg gtagaagatc
    11881 cgttcgcgta tatgatgaaa catacaaaat cccacacgaa gaaatgctgg aaatgatcaa
    11941 agaagcgacg actgcacctt cttctgtcaa tatgcaacca tggcgctttg tggtcgctga
    12001 aagcgaggaa gcaaaagaaa tcttgcgtcc tttgatccgt ttcaatacga gacaaaatga
    12061 tacttcttct gcaatggtca tgatttttgg cgatatgcaa tgttacgaat atggcgaaga
    12121 aatctataac caagcttacg aatcagggaa gatgccaaaa gaagtcagag atcaacaatt
    12181 agctgcgatc attccttatt acaagagttt ttcaagagaa gaaatgaacg atgtagtcaa
    12241 agtcgattct agcttagcgg ctatgcaatt catgttagtt gcgcgtgatc atggctatga
    12301 aacaaatcca attggtggat tcgaagcaga tcagttggca gaggcatttg gtttggataa
    12361 agaccgttat gttccagtta ttattttatc aataggaaaa gcggtagaag aaggttacga
    12421 gtctgttcgt ttagcaccag aaaagatcac tacgttcgaa taaaaggttt tcagttacta
    12481 ttcagtagct attgcttttt ttgaatcgtg tgtggtaacc taataaaaaa ggaggtcgga
    12541 aaaatgactg catcgatgaa attacgtttc gaacttttga ataacaacta aataaattca
    12601 aaagacatgt ataaacaaca atttcatgga tggagtatgt ccattttttc gaacgacagt
    12661 agaataggtg aaagctatga cagatttggt gatttagtca tagttatttc gttcagagta
    12721 ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc tgctcttttt tagtatcgat
    12781 aattgttgtt ttacatgaaa taggagtgta aaacatatgg aaaatttagc agtaaatata
    12841 acaaatctac aagtcagctt tggcaaccag ctagaactat ctattgattc tcttcgtgtc
    12901 tatcagcaag atcgtatagg gatcatagga gaaaatggtg tcggtaaatc gactttgctc
    12961 aaactaatag ccggtgaact ttttcccgat catgggaaag tccaaacaga gatcaccttc
    13021 aactacctgc ctcaattaac ctatctcgct gaagcaaagg acctaaattt ggaattagcc
    13081 agtcatttcc agttaagact ggaagaaact tcagagcgga aatggagcgg aggggaagaa
    13141 cgaaagatcg agttgatacg tcttctttct tcttatgaac aagggatgct tctagacgag
    13201 ccaacaaccc atctagatag aaaaagtatt gatcgactga ttgaagagct tcgttattat
    13261 tatggtacgc tggtttttgt tagtcatgat cgctattttc tagatgagct agcatcgaaa
    13321 atctgggaag taaaagacgg agaaatccga gagttttcgg ggaattatag tgcctatctc
    13381 actcaaaagg aattggagaa aaagactcag ctacgagaag cagagtcgat catgaaagag
    13441 aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa
    13501 gtgtccagta aaaagaaaaa gcaacaaatc agaccggatc ggttgtcttc ctctaaacaa
    13561 aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa cattagagag aagactccaa
    13621 aaaataggag aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa
    13681 tctcttgaac tccacagccg ctatcccatc atgggacaaa atgtccaatt ggaaagaagt
    13741 gggagaacat tactggtaaa tggtgatttt cagttttctt taggtaaaaa aatcgcgatt
    13801 gtcggtgaaa atggttcagg taagacaacc ttattagaac atatccgcaa acaaggagaa
    13861 ggaatccttc tctctccgaa agtaagcttt caagtatatc agcaaaaggg ttatcaaatg
    13921 acatctgaag aatccatcat tcgttttgtc atgagacaaa cagagttttc agaatcgctt
    13981 gtccgtagtt tgctgaatca cttagggttt gctcaggaaa ctttgacgaa accgttatgt
    14041 acattaagtg ggggagaagc gacccgtctg acgattgctt tgctttttac taagccaagt
    14101 aatgtgttgc tgttagatga accgactaat tttattgata tggcaacgat cgaagcttta
    14161 gagaagctaa tgcaaatata tccgggaacg attttgttta cttctcatga ttcttacttt
    14221 gtcgagcgta cggctgatga agtttatgaa ataaaagggc agaaaataaa aaaagtactt
    14281 acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg agtggtacag
    14341 attgcctaca aataaaaaag aaggaataat ttttacaaca atgatgtgtt ttttgatggt
    14401 cttggggatg agcgcgtata atttatggct gcatcaagat cttcatttat caagtttgtt
    14461 gattggtcta gtgcctggat tcgtggtggc tttcattttg gatgtcttca tcatcggtgt
    14521 gctggcgaaa aaaattgcct ttaaactacc agtaaatcca gaaagcaagg tccaaatgat
    14581 cttaactatt tcatgcttga tggttttagg aatggtgaca tgtatgtctt tattcggtgt
    14641 gatcatggaa caaggaatac ctgatcaatt attctctgct tatatgaatg cttggaaaat
    14701 gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt
    14761 gctaaaagca attcaaagta atgaatgatg gaacactgtt atttgaaaaa aggccttggc
    14821 atgctttttg tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg
    14881 ttttggcctg tcgttcggat cgctttttct tttccaactg tattgctctt ttattgaaga
    14941 taatttgctc ttcaatagat atttctagaa aggttctgtt tatcccttac tcaaaaaact
    15001 gctgcatttg ttctcttact tgctgacacc attggagttc ctgctgacaa agcataattc
    15061 tgcgctggat cacaacataa cgtccaaatt cttgtttctt gtctagcgag aggtcattca
    15121 attttttttg atttttttgc atgattttgt tcagctgttg ttctctagtg aatagcagtc
    15181 cagtagctgt tgttttatcg agcgtcgaaa aagcatagat ttttgctaga aattcatctt
    15241 tttgcgtagg agcgggagtt tcctctttga tccattcctc tacaagagca cgtccttcat
    15301 cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt gtccaaaacg gtcacataac
    15361 cctgctggtc taacttggca agcgttgtat agatctgact gtgatgggct tcccaaaaaa
    15421 ttgtgatcag ctgcttcaat tcatacccag aacgtggttt ggataaaagc acgcataata
    15481 atatataggc taaagtattc ataaaaatcc tacctctcgt cttattttct ttattatatc
    15541 acagtaaagt ttccacgttt tacatgtgca tatttacatg taaaataaat catgtatgat
    15601 gctaatagag agaaagggat ggaaatattg gagaatcaaa tagatataca agaacaaaca
    15661 aatgaagaaa caaaggggaa aagttcagcg ctagttgtag cagctacctg tatgatcgct
    15721 tttatgggag tgggtctagt agacccaatc ttaaaaacga ttgctgcaca gttaaacgct
    15781 acgccagctg aaacgaccct gttatttact agttacatgt tagtcactgg tgtggtgatg
    15841 ctatttacag gtttcttatc tagtcggatc ggtttgaaaa aaacattgat gacaggattg
    15901 tttatcatcg ttgtattcgc tggtcttggc ggattatctg ggaatatcgg aatgctgatt
    15961 gggttacgtg ccggctgggg tgtcgggaat gctttatttg tctctactgc tttagcaacg
    16021 atcgtcagta ttttagttgg taacacagaa aaagcaatca tgatgtatga agcagcactt
    16081 ggctgcggaa tggctgttgg ccctctagtc ggtggggtct tgggcagtgg ttcatggcgt
    16141 tatccatttt ttggggtagc atttttgatg ttttgcggtt ttatagccct agcggtactt
    16201 ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt gggaaggtgt caaagcgtta
    16261 agcaatcata aacttcgttc agttggacta atcgcattgt tatataattt cggattcttt
    16321 actttacttg cgtatgcgcc atttttattg attggctatt ctgaattaga agtcggtttt
    16381 gtattttttg gctggggtgt gcttctggct attgcttcga ttttcgttgc accggctctt
    16441 gagcggaagt ttacgactta tcggacaatt ttagcagcat ttgtcttatt cattatttgt
    16501 ttgctgttat taggagctgg aacttctatg ccagtccttg taccgatcag cattgtgtta
    16561 gcaggatttt tccaaggaat tatcaataca ctgttgacga cgattgctat ggaaatacca
    16621 ggtctgcaac gaaacgttgc ttcgtccagc tatagttttg tccgcttttt cggtggagct
    16681 ttagcaccat ttattgcagg tgtgattggt gaacatattg gtgaaagcta tgcgttttat
    16741 tttgcaggtg ccattgttct tatcagtgta tgttgtatca tttatcaccg taattatttt
    16801 gctacaaatg aagaagaata agaaatcaga ggttgtgaaa aagctgtcct gcatcaagtc
    16861 ataagaa
//
LOCUS       DACEMS010000269        12280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.086, whole genome shotgun sequence.
ACCESSION   DACEMS010000269 DACEMS010000000
VERSION     DACEMS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.086"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            289..564
                     /locus_tag="IW735_002786"
     CDS             289..564
                     /locus_tag="IW735_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1045721.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            577..2655
                     /locus_tag="IW735_002787"
     CDS             577..2655
                     /locus_tag="IW735_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1045722.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2815..4701
                     /locus_tag="IW735_002788"
     CDS             2815..4701
                     /locus_tag="IW735_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1045723.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4713..5660
                     /locus_tag="IW735_002789"
     CDS             4713..5660
                     /locus_tag="IW735_002789"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1045724.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5680..6207
                     /locus_tag="IW735_002790"
     CDS             5680..6207
                     /locus_tag="IW735_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1045725.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6270..6923)
                     /locus_tag="IW735_002791"
     CDS             complement(6270..6923)
                     /locus_tag="IW735_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1045726.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7055..7897
                     /locus_tag="IW735_002792"
     CDS             7055..7897
                     /locus_tag="IW735_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1045727.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8055..8951
                     /locus_tag="IW735_002793"
     CDS             8055..8951
                     /locus_tag="IW735_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1045728.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8954..9667
                     /locus_tag="IW735_002794"
     CDS             8954..9667
                     /locus_tag="IW735_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1045729.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9687..10205
                     /gene="msrA"
                     /locus_tag="IW735_002795"
     CDS             9687..10205
                     /gene="msrA"
                     /locus_tag="IW735_002795"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1045730.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10202..10429
                     /locus_tag="IW735_002796"
     CDS             10202..10429
                     /locus_tag="IW735_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1045731.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10581..11132
                     /gene="lepB"
                     /locus_tag="IW735_002797"
     CDS             10581..11132
                     /gene="lepB"
                     /locus_tag="IW735_002797"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1045732.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11215..11757)
                     /locus_tag="IW735_002798"
     CDS             complement(11215..11757)
                     /locus_tag="IW735_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1045733.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11994..12248)
                     /locus_tag="IW735_002799"
     CDS             complement(11994..12248)
                     /locus_tag="IW735_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1045734.1"
                     /translation="MLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQI
                     AKENGGTFHEVDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         4272 a         2093 c         2471 g         3444 t
ORIGIN      
        1 tgattcatca gtcccataaa ttatagagcc gatttttgtc gcagccccat ttttttgttg
       61 tgataaaaga acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt
      121 atctttctta agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa
      181 atttatggaa atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa
      241 aagaactttg ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt
      301 tttgaacaac aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc
      361 cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt
      421 ctgaaacatg atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac
      481 caaacacagg ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa
      541 atgcttacac aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt
      601 gtatttgcag taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca
      661 gctccactga tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca
      721 atcgattttg aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa
      781 ggagaaaaag cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca
      841 tttggaggag tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca
      901 agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta
      961 gcagtcagtt ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa
     1021 ggagaaggtc ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg
     1081 ctgatcaccg gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt
     1141 gggggagctt tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc
     1201 gaaaaagcat cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt
     1261 ctagcctatg tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca
     1321 ggtattctcc aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc
     1381 ttgtcccaca gtacatggac aactattgct tttacactga atgcattagt atttatattt
     1441 ttaggaatcg aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac
     1501 ggtcttttat tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt
     1561 tctatcagtc ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac
     1621 gagatcttga tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt
     1681 attttgccat tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg
     1741 acggcgggtg tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg
     1801 gaaacagagg aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt
     1861 gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact
     1921 gaagcagtga tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc
     1981 gatgatgaaa agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat
     2041 ggattggatg aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc
     2101 cgttttcttt ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt
     2161 tggttcattg ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa
     2221 agaagatatg ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag
     2281 acatatcaaa aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat
     2341 gatgaaacga tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac
     2401 tcggggaatt tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg
     2461 ctaagaggtt attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc
     2521 acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg
     2581 aacaaaccat ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg
     2641 agtccaataa aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga
     2701 acaggatcga atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa
     2761 attttcctga agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg
     2821 aaagaattaa aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag
     2881 atttcttttt tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt
     2941 aaaacaagtc tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca
     3001 gtgttttatc caactgatta ccgtattggt tatctttctc aagcagctga attttccgat
     3061 gaattaactg ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga
     3121 gcatatgagg aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa
     3181 cgttatgcta aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac
     3241 gcaaagatta ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta
     3301 tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta
     3361 ctattactag acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac
     3421 tatttgaaac aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat
     3481 agagtagcaa accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat
     3541 tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa
     3601 aagcgcaagc ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt
     3661 ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa
     3721 gtaaaccaag aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta
     3781 ctggaaatca aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat
     3841 ttattgattc aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg
     3901 acattgctta atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt
     3961 gaaacggttc atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg
     4021 atgattgctt acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc
     4081 ggagtagcag aattacttga acgattttta tttccaagat ttatgcatgg gacactgata
     4141 ggcaaacttt caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa
     4201 cctaatgttt tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt
     4261 ttggaagatt attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat
     4321 ttcttggaca aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt
     4381 tattacggaa acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa
     4441 aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg
     4501 gaacaaaaag aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca
     4561 ttgctccaag aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa
     4621 aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg
     4681 agtgaatggg tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa
     4741 gaaaattgtt ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct
     4801 tattcggata ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac
     4861 gagtaccttt tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata
     4921 tccgctatct attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca
     4981 tcaaaagtcc agactataat ggaccagata tgactgattt tggccataga tgtttgacag
     5041 atcctgaatt caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa
     5101 ccgatgattc ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc
     5161 gtcactggga aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa
     5221 tcaaaaagac tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc
     5281 ctagcatggc tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc
     5341 taagctgcca attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg
     5401 caagttatgc gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat
     5461 ttgttcacac attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac
     5521 aattgagccg agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag
     5581 tatttgattt tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca
     5641 aagctcctat cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca
     5701 caggatgaaa acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat
     5761 ctgaaattct tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc
     5821 gaagggatgg gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct
     5881 tcctataaag cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa
     5941 aaagaaacag acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg
     6001 ccattttgta cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat
     6061 tttccacctg ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat
     6121 gaaaaaaatc gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa
     6181 agcgggacag gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag
     6241 ctgtcccatc catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata
     6301 aaacctgcac ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc
     6361 aagctataaa agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca
     6421 ccagtcattc caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata
     6481 aaaatataga tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct
     6541 ttatatataa tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc
     6601 catcctcgga tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc
     6661 gaactatgat cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac
     6721 aaagtagacg ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata
     6781 tggagggagg aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc
     6841 aatccagtcc caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt
     6901 cgattaaatt tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa
     6961 tcattatttg tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata
     7021 aggcatcttg ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga
     7081 ttcttcatgt acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct
     7141 atccatcatg atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt
     7201 tatggaaatg atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga
     7261 attcatctcg atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat
     7321 gacaaaaggc ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac
     7381 gaaagtaacg gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg
     7441 agcagctgaa ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa
     7501 agtacaagac aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg
     7561 tggaagaatc agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat
     7621 gaatttcgcc cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga
     7681 taaatggttt aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg
     7741 tatttcacat gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt
     7801 attcccggac atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg
     7861 taaagacgcg tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag
     7921 cctgaaaggc ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc
     7981 ttttttcata aagactacct ttttgataaa atttttgata acattagaac caatcgaata
     8041 caagaggtga aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat
     8101 taattgcgat catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa
     8161 tgaaaccagt aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag
     8221 ctatcggaga ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc
     8281 cccttgtcgc aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact
     8341 atggtgtaaa tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa
     8401 ttcgaaacaa tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga
     8461 tgaaagttat ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga
     8521 aaaactatca ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg
     8581 cacctattta cattttaggc atctacaatc cattttattt gaactttcct gagatcactg
     8641 acatgcagac aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc
     8701 cgaactgcta ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag
     8761 gtgtcacaga agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca
     8821 aaaataatgt gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga
     8881 tggcaaatgc agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg
     8941 atgatcaata aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa
     9001 ttactggaaa ttcgccttcc ttatccttct tggggtagta cttggttcga ccgtattttt
     9061 aggcagccgt atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga
     9121 aagacaagga gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat
     9181 cagctttttc cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga
     9241 aaatgaagca ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt
     9301 atattttgat ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc
     9361 tatcggaaca ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa
     9421 attaccagat tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca
     9481 attccgaatg caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga
     9541 cattcggttt agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac
     9601 caacaaaact agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga
     9661 tgagtagtat tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct
     9721 ggtgtatgat ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata
     9781 ctggagggca tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata
     9841 cagaagcagt ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac
     9901 tttactggca gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca
     9961 attatcgccc ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta
    10021 agaaacagtt agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag
    10081 cccaaccatt ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa
    10141 attttaaaag aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag
    10201 ggtgaatcga agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc
    10261 tttatctgtt ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag
    10321 ctacgaagaa atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat
    10381 ctttgacgaa gcatgggaaa aatatttaga aaataatcat gacatataag agatagtttt
    10441 gtactttatg atcaaaaaag ttttttgtcc aatagcctaa ttgaaagaat tatttttatt
    10501 tcagctatct agtcgcattt ttttgatatt tttcttataa taaagaaaga atgaaaaaaa
    10561 gagggatgga ttgatagaga ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg
    10621 tattcaaata cttacttgtt tctgtattcc ttgcgttcat gttgcgggga tttttattca
    10681 tcccggtacc agtggaaggc aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg
    10741 tgatggaaaa attttcggag atcagacgat ttgatattgt tgtttttcaa ttagcagatg
    10801 gcacgattta tatcaaacga gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg
    10861 atcaattgaa gatcaatggg aaagtagtta aggaacctta tctaactaaa aacctgaaat
    10921 cagatcatgc aaatgcatcc tatacgacgg actttacact acaagagcta acgggacaat
    10981 ccaagcttcc ggaagataat tattttgtac ttggagataa tcgaagagtt tcaaaagata
    11041 gtcgttcgtt tggcacgatc aataaaactg atattctagg taaagcaaga tttgtctatt
    11101 atccacttga tgaaataaaa tggatccagt gagaaacaat aaagcgtgca gtctgttgag
    11161 actgcacgct ttattgttta atccaaaatc agtttactac cttagtacgt aaaatcaaac
    11221 agctcgttta aacacttttc tagcctctaa agttttgata aatgaataaa caaccattat
    11281 ttgaataggg atttggatca tattcctgat gaatctacct agaaaaatta ctttgaaatc
    11341 tgcaccaaac aaaacagaca tcagccaaga attgataaaa aggctgactg ggatcataat
    11401 caataattgg gcagcgatga cggaaggcag gtttatcttt ttatgatata aaaataagcc
    11461 attaataaat ccaccaagga aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc
    11521 aaatatcgtg aaatttaaag tgttcgacaa catttcaagc aaaccagccc agatcggtcc
    11581 atatagataa cccataagcg ccatacttaa aaatccgaat gtgatttgaa aataagcagg
    11641 accaatcgct atcttgtata agatgacatc aagggctaat aaaagagcag aggtcgacaa
    11701 caatcttgca ttcatttcgg gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat
    11761 attctctcct ttgtcagtta ataataaaag gataccataa aatcaccaat gtgtcatgac
    11821 acattggtga tttacataca ctggcatcta acaattaaaa aaagggtgag aagactgctt
    11881 ttataaactg taaaaaagag attgtacgaa taaaaaaaat agaggcggag aatacttatc
    11941 gtatcatccg cctttgtttt tttgttataa aaaacgtcag attagttatc tgcttacatc
    12001 ttcatccata cgggttctaa tgtatttccg tctagatctt ggacttctaa tgaaaacatt
    12061 tcttcttcag gcattcccat atctacttcg tggaaagtac caccattttc ttttgcaatt
    12121 tgagcaaatt tttttacttc atccgcattt tcaacactaa acgaaatcaa agcaccactt
    12181 gtttttttat tatcagctat ttctttatct tttataaatt ttttataaaa atcatgagag
    12241 agaagcatta tccaaaaagt gtcatcccat accattgaac
//
LOCUS       DACEMS010000270        11422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.090, whole genome shotgun sequence.
ACCESSION   DACEMS010000270 DACEMS010000000
VERSION     DACEMS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.090"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            39..977
                     /locus_tag="IW735_002800"
                     /pseudo
     CDS             39..977
                     /locus_tag="IW735_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="IW735_002801"
     CDS             980..3163
                     /locus_tag="IW735_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1045735.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="IW735_002802"
     CDS             3160..4161
                     /locus_tag="IW735_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1045736.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="IW735_002803"
     CDS             4176..5090
                     /locus_tag="IW735_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1045737.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="IW735_002804"
     CDS             5335..5451
                     /locus_tag="IW735_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1045738.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW735_002805"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW735_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1045739.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW735_002806"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW735_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1045740.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="IW735_002807"
     CDS             9421..10683
                     /locus_tag="IW735_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1045741.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11410
                     /locus_tag="IW735_002808"
     CDS             10928..11410
                     /locus_tag="IW735_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1045742.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         3771 a         1907 c         2388 g         3356 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aa
//
LOCUS       DACEMS010000271         8104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.113, whole genome shotgun sequence.
ACCESSION   DACEMS010000271 DACEMS010000000
VERSION     DACEMS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.113"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(134..577)
                     /locus_tag="IW735_002809"
     CDS             complement(134..577)
                     /locus_tag="IW735_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1045743.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IW735_002810"
     CDS             complement(795..2135)
                     /locus_tag="IW735_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1045744.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IW735_002811"
     CDS             2404..3300
                     /locus_tag="IW735_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1045745.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IW735_002812"
     CDS             complement(3450..4391)
                     /locus_tag="IW735_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1045746.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IW735_002813"
     CDS             4517..5638
                     /locus_tag="IW735_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1045747.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IW735_002814"
     CDS             5896..7398
                     /locus_tag="IW735_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1045748.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IW735_002815"
     CDS             7465..7992
                     /locus_tag="IW735_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1045749.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2689 a         1511 c         1599 g         2305 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaaaaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaat
//
LOCUS       DACEMS010000272         7482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.119, whole genome shotgun sequence.
ACCESSION   DACEMS010000272 DACEMS010000000
VERSION     DACEMS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.119"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..192
                     /locus_tag="IW735_002816"
     CDS             109..192
                     /locus_tag="IW735_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1045750.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IW735_002817"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IW735_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1045751.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IW735_002818"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IW735_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IW735_002819"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IW735_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IW735_002820"
     CDS             2348..2593
                     /locus_tag="IW735_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1045752.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IW735_002821"
     CDS             2696..3565
                     /locus_tag="IW735_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1045753.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IW735_002822"
     CDS             3546..4280
                     /locus_tag="IW735_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1045754.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW735_002823"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW735_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="IW735_002824"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="IW735_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1045755.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="IW735_002825"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="IW735_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1045756.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6759..6947)
                     /locus_tag="IW735_002826"
                     /pseudo
     CDS             complement(6759..6947)
                     /locus_tag="IW735_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6927..7469
                     /locus_tag="IW735_002827"
     CDS             6927..7469
                     /locus_tag="IW735_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1045757.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2623 a         1131 c         1567 g         2161 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     6721 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     6781 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     6841 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     6901 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta
     6961 ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa
     7021 gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat
     7081 ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta
     7141 cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg
     7201 aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg
     7261 gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac
     7321 agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa
     7381 gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag
     7441 agccggttct gttgcaaagt tttaaataaa gaataaaatc cc
//
LOCUS       DACEMS010000273         5680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.139, whole genome shotgun sequence.
ACCESSION   DACEMS010000273 DACEMS010000000
VERSION     DACEMS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.139"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            319..885
                     /gene="amaP"
                     /locus_tag="IW735_002828"
     CDS             319..885
                     /gene="amaP"
                     /locus_tag="IW735_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1045758.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            898..1086
                     /locus_tag="IW735_002829"
     CDS             898..1086
                     /locus_tag="IW735_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1045759.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1099..1656
                     /locus_tag="IW735_002830"
     CDS             1099..1656
                     /locus_tag="IW735_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1045760.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1688..1930
                     /locus_tag="IW735_002831"
     CDS             1688..1930
                     /locus_tag="IW735_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1045761.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2271..3485
                     /locus_tag="IW735_002832"
     CDS             2271..3485
                     /locus_tag="IW735_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1045762.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3516..3734
                     /locus_tag="IW735_002833"
                     /pseudo
     CDS             3516..3734
                     /locus_tag="IW735_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4153..4626
                     /gene="dfrF"
                     /locus_tag="IW735_002834"
     CDS             4153..4626
                     /gene="dfrF"
                     /locus_tag="IW735_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1045763.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            4753..5409
                     /locus_tag="IW735_002835"
     CDS             4753..5409
                     /locus_tag="IW735_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1045764.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            5591..>5680
                     /locus_tag="IW735_002836"
     CDS             5591..>5680
                     /locus_tag="IW735_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008750783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HAQ1045765.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHG"
BASE COUNT         1948 a          831 c         1189 g         1712 t
ORIGIN      
        1 ctaaagttat ttattgtatt ttttaattct ccgaaaaaat ttaataaata aatagaatct
       61 tgtaataata actgagcata atctttgcaa tttttaatct tgaataatat cctttaagta
      121 tagccagcaa tcaaaagtaa atagagaata agtaagcaat tgttatttca gtatcagact
      181 ggcaataaaa cgccgttatg atccagcaat gtttggtagc gctttattta ctgatgctat
      241 gctaactgtt cgctatctat actgtgattg tcggtagagt tatttacagg gtaaaaataa
      301 gtttatagga ggtggaaaat gaataagggg ttaaaaataa tcggtgttat catgagttta
      361 ctgctacttt cagtatttct agcgattgcg ttgatcaatg cgccgtatat tatgcctgaa
      421 caattggaac gatttcgctt ctttacttta acgaattact acatgcaaca atatattttc
      481 tgggcagctg tagtatttgc cgttcttgct attatcatct tgctagtcgt tcttttctat
      541 ccaaagtcaa gaggaacttt cgttatgaaa cgggaagatg gcaaactaac aatcgacaaa
      601 aaagcgatcg aagggttggt acgttcccat ttacacgaag aagagttcat tcattcacct
      661 aaagtacgga tacgttcaac gaaaaatcga atccatatcc atgtcaacgg agacttgaaa
      721 cgaacttctt cattagtagg gaaaacaggt gtattgatgc aagatatcga agaagaagta
      781 actaaagtac tcggaacaaa agaaacggtc aaagtggcag tcacatacag cggttatgaa
      841 gaacaagaag atacaagaga ctacaagcac tctcgagtgg aataggaggg ccaaataatg
      901 aaagaactat tttcagcata caagctccct attatctttg ggggtcttgg actactgcta
      961 gctgttttat ggttaacgat tggatttttc aagacattgt tgatcataat catgacagca
     1021 attggcgcag gaatcggttt ttatttgaaa cagtcaggaa tagttgatca attttttaaa
     1081 tcataggagg aaacaaagat ggaaaacaaa aacatgaata caacaaatgg atcagttttg
     1141 gcaaatgaaa aaggagaaaa ccgcatgatg aatggtgaat tgacttatga agacaaagtc
     1201 atccaaaaaa tcatcggtta tgcattgaaa gatgtagatg gtctattgac attagatggc
     1261 ggatttttct caaatatggc tgaaaaatta gtcaacacag ataacgtaac agcaggtatc
     1321 aatacagaag taggtaagaa acaagtagct gtggatctag atgtgatcgt agaatacggt
     1381 aaagacatcg aagatattta caaccaaatc aaagaattga tcagtactga agtaaacaat
     1441 atgacacatt tagatgttat cgaagtgaat gtgaatgttg cagacatcaa aacacaagaa
     1501 gaataccaaa aagatcaaga aacagtacaa gacaaagtaa cagaagcagc aaaatccaca
     1561 ggacaatttg cttcacgcca aacagataaa gcaaaatctg ctgtaggcaa aggtgcacaa
     1621 aaagtcaaag aaaataacga accacgtgta caataagaag taagaagtca gttaggagga
     1681 atttaaaatg ggatttattt gggcattaat cgtcggcggt atcattggag caattgcagg
     1741 agcaatcacg aataaaggat catcaatggg gatcatcgca aacatcattg ctggtttagt
     1801 aggttcagca attggacaag ctttactagg aacttgggga ccaacaattg caggaatggc
     1861 tatcattcca tcattgatcg gtgcaatcat tttagtatta gtcgtttcat tctttgcacg
     1921 taaattataa gcaaattgaa gaaaaactta tgttgaaatg aagaacaaat ttgagaaccg
     1981 tagcgacact cgctgcggtt cttttttata tattttatat gaagtattct ttattcctta
     2041 tgaaaaaata tctttaaaaa taaaaaaaga cgaaaataaa cggttttcta taaaaagaag
     2101 accgtttatt ttttgtcttt atttgtgtga aaaacgaaaa agagcacaac acatcaagct
     2161 tttattaatt gtctgtcttt tataaatgca agtattaatt caaaaaaaca gaagaaaaaa
     2221 acaactacac cttcttttat actcgtaatc gtagaaagga ggcgaaaaaa atgaatacat
     2281 tacctatcaa tacaggagat acagcattca tgattttatg tacagcaatg gtttgcctga
     2341 tgactcctgg tctagctttt ttttacggcg gattagcacg gaagaataat atactaacga
     2401 tcatgtctca atcttttctc tccgttggga taacaacgat tatgtgggta tttggtggat
     2461 tcggattggc ctttggacgg gatatcggcg gtgttattgg aaacccggca gatttctttt
     2521 tgatgagaca tgttacaagc ttccccaaca tgtttcatgg tgcaacgatt ccatttttaa
     2581 tgttctttct ttaccaactg atgtttatcg tgattaccgt tcctttgatg acaggagctt
     2641 ttgcagggag attgaattta aaaggctata ttatacttgt tattttttgg aatctgctga
     2701 tttatttccc tgtttgccac tggatatggg gtggcggatt tttggatcag atgggattta
     2761 gagatttcgc tggtggagca gtcatccaca cgacagctgg attcggctct ttagcatgta
     2821 tcctcacact tggtcaaaga aagattcctt taggagaaag aagtcagcat agcagtctta
     2881 tggcagctgc tatcggtaca ggactgcttt ggtttggctg gttcggtttt aattccggtg
     2941 gtgcacttcg agcagatgaa caagcagtca atgcatttgg ttcaacattc atcgctcttg
     3001 catttgcgat gattacttgg ctgatcattg caaaagtaaa aggaaatggc tttgattttg
     3061 tcgacgtact aacaggatcc gttgctggat tggcagccat tacgccttgt gccggttatg
     3121 tagaagcaaa aagtgccatg ctgattggga tcattgcggg tatcgtctgt catgctgctg
     3181 ttgattttcg taaaaagaaa caatgggatg atgcacttga tgtgtggggt gttcatggca
     3241 tgggcggatt cacaggtacg attctgatag ggatctttgc atctaacagc catttactaa
     3301 cgaatccaag cagctggtac ttcttaggga ttcaagtgat tggtgtcatc attactgccg
     3361 tgtatgccta tgtgctgaca acagtgattt taaaatcggc gatgcacttt acaacgatca
     3421 ctactacaaa agaagaacaa gaagaaggat tggacttttt cttctttggc aacaaactca
     3481 attgaaacaa attcaaatag aaaagaggaa gattcatgca acaaagcaaa acgaaaattc
     3541 ctgcaaaaaa attaacgttc tttggctttt tggctatgac catctcaatg gtcgtttcat
     3601 tatatgaata tcccacattc gcgacctcag gcttcagctt agtatttttc ttgttattag
     3661 gcggtttgct atggtttatc cctgtggcat tgtgcgcagc agagatggca acggtgaaag
     3721 gatgggaaaa aggtgtttcc caaacaaaaa gatattaaga aacagcccat gtttacctgc
     3781 ttgcaaacaa aagaatattg ctactagact aagagatact acataagtgt gttggtatct
     3841 cttttttgtt gccgtgaaac agcagactat ttcatttctg cttgattacc ttaaaactta
     3901 ttagtctatg gtttgtcaag ggcttgttgc gtgagaaatg ctgaatatca ccctttacaa
     3961 aacataggct gataagtaac ttttcaaaag cagaaaagaa atggcaatgc ttataacaat
     4021 atgatataat gcagatgaag acaaaaaagg aatttgtcga gctggttagt tcgagataat
     4081 tttgagtttg ttaagatgtg gtgcacttga tggaatcaca ggtacgacat catattatgg
     4141 aggatgaaat agatgatagg tttgattgtt gcgaggtcaa agaataatgt tataggcaag
     4201 aatggtaata taccatggaa aataaaggga gaacaaaagc aatttagaga gttaacaacg
     4261 ggtaatgtgg ttattatggg gcgaaagtct tatgaagaaa tcggtcatcc gttgcctaat
     4321 agaatgaata ttgttgtttc caccacaaca gagtatcaag gagataattt agtttcagtt
     4381 aaatcattag aagatgcatt attattggct aaaggacgag atgtatacat atctggtgga
     4441 tatggactat ttaaggaagc tttgcaaata gtagataaaa tgtatatcac agaagtagat
     4501 ttaaatattg aagatggaga tacattcttt ccagaatttg atatcaatga ttttgaagtt
     4561 ttgatagggg aaacacttgg tgaggaagtg aaatatacga gaacatttta tgtaaggaaa
     4621 aaatgaattg agtaaatttt ggatttaggg tgttttcata aatatattgc tttttatgca
     4681 tatataaatt gttgtaagac aaattccagt ttgtatgggg cgataaaatc agaatttgag
     4741 agaggattga atatgaataa ggaatggtct gaacttaata aaacaatgca ggcacaaata
     4801 aaaaagaaag atacttataa gaggggtatt gatactttac ttactttacg aagtcagtta
     4861 atacaaacct tagtatcttt caaagaagaa ttatgcagag aagattttaa ctcaatcccc
     4921 ttcataaacg ctgatggtta tcatagtaag acgattgctt attctatttg gcatattttc
     4981 cgcattgaag atatagttgt gcatacagtg ataaatgaag atgaacaagt attttttgca
     5041 ggcaattatc aagagcgtat caattcacct atcattacaa caggaaatga actcatgaaa
     5101 cagcagattg cagacttttc aaaacagctt aatctggaag aattatattt atatattttc
     5161 gaagtatggg aaagcactga gaaaatgctt gaacggttat cttatgatga attgaaaaga
     5221 aaaataccga aagaaagaaa aggatactta gaatcgttga atgtagtaaa cgacaatgaa
     5281 aaagcaattt ggctgattga ttattggtgt aataaagata tttgtggact aattcaaatg
     5341 ccgttttcaa gacattggat tatgcacaca gaagcctgtt tgcgtataaa gaacaagata
     5401 cattcatagg caaaaatttc agtttgtaga actaaggaaa ttggaattta aggaggaata
     5461 aagtatggtt agtgatttta taagagcagc atttccatgg attttaatgg gtttatttgt
     5521 agcaatcagt tgttccctta tgagtaaaaa agagaaataa attcctgttt gtatttataa
     5581 aagagaaaaa atgagaaaag tagttttatt tattgccatg agccttgacg gatatattgc
     5641 ggacggtaat ggtggagtag cttggctaaa tggtcatgga
//
LOCUS       DACEMS010000274         4995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.145, whole genome shotgun sequence.
ACCESSION   DACEMS010000274 DACEMS010000000
VERSION     DACEMS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.145"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(30..2180)
                     /locus_tag="IW735_002837"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="IW735_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="IW735_002838"
     CDS             2326..2901
                     /locus_tag="IW735_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1045766.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="IW735_002839"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="IW735_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1045767.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="IW735_002840"
     CDS             3788..4942
                     /locus_tag="IW735_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1045768.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DACEMS010000275         2882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.190, whole genome shotgun sequence.
ACCESSION   DACEMS010000275 DACEMS010000000
VERSION     DACEMS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.190"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1096
                     /locus_tag="IW735_002841"
     CDS             128..1096
                     /locus_tag="IW735_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1045769.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1089..2120
                     /gene="vanA"
                     /locus_tag="IW735_002842"
     CDS             1089..2120
                     /gene="vanA"
                     /locus_tag="IW735_002842"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1045770.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2126..2734
                     /locus_tag="IW735_002843"
     CDS             2126..2734
                     /locus_tag="IW735_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1045771.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          852 a          555 c          705 g          770 t
ORIGIN      
        1 ttcttacacg gtagacttaa taccgtaaga acgagccgtt ttcgttcttc agagaaagat
       61 ttgacaagat taccattggc atccccgttt tatttggtgc ctttcacaga aagggttggt
      121 cttaattatg aataacatcg gcattactgt ttatggatgt gagcaggatg aggcagatgc
      181 attccatgct ctttcgcctc gctttggcgt tatggcaacg ataattaacg ccaacgtgtc
      241 ggaatccaac gccaaatccg cgcctttcaa tcaatgtatc agtgtgggac ataaatcaga
      301 gatttccgcc tctattcttc ttgcgctgaa gagagccggt gtgaaatata tttctacccg
      361 aagcatcggc tgcaatcata tagatacaac tgctgctaag agaatgggca tcactgtcga
      421 caatgtggcg tactcgccgg atagcgttgc cgattatact atgatgctaa ttcttatggc
      481 agtacgcaac gtaaaatcga ttgtgcgctc tgtggaaaaa catgatttca ggttggacag
      541 cgaccgtggc aaggtactca gcgacatgac agttggtgtg gtgggaacgg gccagatagg
      601 caaagcggtt attgagcggc tgcgaggatt tggatgtaaa gtgttggctt atagtcgcag
      661 ccgaagtata gaggtaaact atgtaccgtt tgatgagttg ctgcaaaata gcgatatcgt
      721 tacgcttcat gtgccgctca atacggatac gcactatatt atcagccacg aacaaataca
      781 gagaatgaag caaggagcat ttcttatcaa tactgggcgc ggtccacttg tagataccta
      841 tgagttggtt aaagcattag aaaacgggaa actgggcggt gccgcattgg atgtattgga
      901 aggagaggaa gagtttttct actctgattg cacccaaaaa ccaattgata atcaattttt
      961 acttaaactt caaagaatgc ctaacgtgat aatcacaccg catacggcct attataccga
     1021 gcaagcgttg cgtgataccg ttgaaaaaac cattaaaaac tgtttggatt ttgaaaggag
     1081 acaggagcat gaatagaata aaagttgcaa tactgtttgg gggttgctca gaggagcatg
     1141 acgtatcggt aaaatctgca atagagatag ccgctaacat taataaagaa aaatacgagc
     1201 cgttatacat tggaattacg aaatctggtg tatggaaaat gtgcgaaaaa ccttgcgcgg
     1261 aatgggaaaa cgacaattgc tattcagctg tactctcgcc ggataaaaaa atgcacggat
     1321 tacttgttaa aaagaaccat gaatatgaaa tcaaccatgt tgatgtagca ttttcagctt
     1381 tgcatggcaa gtcaggtgaa gatggatcca tacaaggtct gtttgaattg tccggtatcc
     1441 cttttgtagg ctgcgatatt caaagctcag caatttgtat ggacaaatcg ttgacataca
     1501 tcgttgcgaa aaatgctggg atagctactc ccgccttttg ggttattaat aaagatgata
     1561 ggccggtggc agctacgttt acctatcctg tttttgttaa gccggcgcgt tcaggctcat
     1621 ccttcggtgt gaaaaaagtc aatagcgcgg acgaattgga ctacgcaatt gaatcggcaa
     1681 gacaatatga cagcaaaatc ttaattgagc aggctgtttc gggctgtgag gtcggttgtg
     1741 cggtattggg aaacagtgcc gcgttagctg ttggcgaggt ggaccaaatc aggctgcagt
     1801 acggaatctt tcgtattcat caggaagtcg agccggaaaa aggctctgaa aacgcagtta
     1861 taaccgttcc cgcagacctt tcagcagagg agcgaggacg gatacaggaa acggcaaaaa
     1921 aaatatataa agcgctcggc tgtagaggtc tagcccgtgt ggatatgttt ttacaagata
     1981 acggccgcat tgtactgaac gaagtcaata ctctgcccgg tttcacgtca tacagtcgtt
     2041 atccccgtat gatggccgct gcaggtattg cacttcccga actgattgac cgcttgatcg
     2101 tattagcgtt aaaggggtga taagcatgga aataggattt acttttttag atgaaatagt
     2161 acacggtgtt cgttgggacg ctaaatatgc cacttgggat aatttcaccg gaaaaccggt
     2221 tgacggttat gaagtaaatc gcattgtagg gacatacgag ttggctgaat cgcttttgaa
     2281 ggcaaaagaa ctggctgcta cccaagggta cggattgctt ctatgggacg gttaccgtcc
     2341 taatcgtgct gtaaactgtt ttatgcaatg ggctgcacag ccggaaaata acctgacaaa
     2401 ggaaagttat tatcccaata ttgaccgaac tgagatgatt tcaaaaggat acgtggcttc
     2461 aaaatcaagc catagccgcg gcagtgccat tgatcttacg ctttatcgat tagacacggg
     2521 tgagcttgta ccaatgggga gccgatttga ttttatggat gaacgctctc atcatgcggc
     2581 aaatggaata tcatgcaatg aagcgcaaaa tcgcagacgt ttgcgctcca tcatggaaaa
     2641 cagtgggttt gaagcatata gcctcgaatg gtggcactat gtattaagag acgaaccata
     2701 ccccaatagc tattttgatt tccccgttaa ataaactttt aaccgttgca cggacaaact
     2761 atataagcta actctttcgg caggaaaccc gacgtatgta actggttctt agggaattta
     2821 tatatagtag atagtattga agaggttctg ttgcaaagtt ttaaataaag aataaaatcc
     2881 ct
//
LOCUS       DACEMS010000276         2717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.193, whole genome shotgun sequence.
ACCESSION   DACEMS010000276 DACEMS010000000
VERSION     DACEMS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.193"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            363..1274
                     /locus_tag="IW735_002844"
     CDS             363..1274
                     /locus_tag="IW735_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1045772.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1427..1912
                     /gene="vanZ"
                     /locus_tag="IW735_002845"
     CDS             1427..1912
                     /gene="vanZ"
                     /locus_tag="IW735_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1045773.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2199..2528)
                     /locus_tag="IW735_002846"
     CDS             complement(2199..2528)
                     /locus_tag="IW735_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1045774.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
BASE COUNT          936 a          411 c          493 g          877 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccattg aagatgtaag gcagagcgat
       61 attgcggtca ttatctgcgt gcgctgcggc aagatagcct gataataaga ctgatcgcat
      121 agaggggtgg tatttcacac cgcccattgt caacaggcag ttcagcctcg ttaaattcag
      181 catgggtatc acttatgaaa attcatctac attggtgata atagtaaatc cagtagggcg
      241 aaataattga ctgtaattta cggggcaaaa cggcacaatc tcaaacgaga ttgtgccgtt
      301 taaggggaag attctagaaa tatttcatac ttccaactat atagttaagg aggagactga
      361 aaatgaagaa gttgtttttt ttattgttat tgttattctt aatatactta ggttatgact
      421 acgttaatga agcactgttt tctcaggaaa aagtcgaatt tcaaaattat gatcaaaatc
      481 ccaaagaaca tttagaaaat agtgggactt ctgaaaatac ccaagagaaa acaattacag
      541 aagaacaggt ttatcaagga aatctgctat taatcaatag taaatatcct gttcgccaag
      601 aaagtgtgaa gtcagatatc gtgaatttat ctaaacatga cgaattaata aatggatacg
      661 ggttgcttga tagtaatatt tatatgtcaa aagaaatagc acaaaaattt tcagagatgg
      721 tcaatgatgc tgtaaagggt ggcgttagtc attttattat taatagtggc tatcgagact
      781 ttgatgagca aagtgtgctt taccaagaaa tgggggctga gtatgcctta ccagcaggtt
      841 atagtgagca taattcaggt ttatcactag atgtaggatc aagcttgacg aaaatggaac
      901 gagcccctga aggaaagtgg atagaagaaa atgcttggaa atacgggttc attttacgtt
      961 atccagagga caaaacagag ttaacaggaa ttcaatatga accatggcat attcgctatg
     1021 ttggtttacc acatagtgcg attatgaaag aaaagaattt cgttctcgag gaatatatgg
     1081 attacctaaa agaagaaaaa accatttctg ttagtgtaaa tggggaaaaa tatgagatct
     1141 tttattatcc tgttactaaa aataccacca ttcatgtgcc gactaatctt cgttatgaga
     1201 tatcaggaaa caatatagac ggtgtaattg tgacagtgtt tcccggatca acacatacta
     1261 attcaaggag gtaaggatgg cggaatgaaa ccaacgaaat taatgaacag cattattgta
     1321 ctagcacttt tggggtaacg ttagcttttt aatttaaaac ccacgttaac taggacattg
     1381 ctatactaat gatacaactt aaacaaaaga attagaggaa attatattgg gaaaaatatt
     1441 atctagagga ttgctagctt tatatttagt gacactaatc tggttagtgt tattcaaatt
     1501 acaatacaat attttatcag tatttaatta tcatcaaaga agtcttaact tgactccatt
     1561 tactgctact gggaatttca gagagatgat agataatgtt ataatcttta ttccatttgg
     1621 cttgcttttg aatgtcaatt ttaaagaaat cggattttta cctaagtttg cttttgtact
     1681 ggttttaagt cttacttttg aaataattca atttatcttc gctattggag cgacagacat
     1741 aacagatgta attacaaata ctgttggagg ctttcttgga ctgaaattat atggtttaag
     1801 caataagcat atgaatcaaa aaaaattaga cagagttatt atttttgtag gtatactttt
     1861 gctcgtatta ttgctcgttt accgtaccca tttaagaata aattacgtgt aagatgtcta
     1921 aatcaagcaa tctgatcttt catacacata aagatattga atgaattgga ttagatggaa
     1981 aacgggatgt ggggaaactc gcccgtaggt gtgaagtgag gggaaaaccg gtgataaagt
     2041 aaaaagctta cctaacacta tagtaacaaa gaaagcccaa ttatcaattt tagtgctgag
     2101 gaattggtct ctttaataaa tttccttaac gttgtaaatc cgcattttcc tgacggtacc
     2161 cccaaactaa gatttcatag atagattttc tgataacttt aaaaaacacg aatagcggaa
     2221 aggatgtctt cattcgctaa agagtaataa actacttttc catcttgacg tgattttgcc
     2281 attccatgtt tttttaaaaa acgtaaatgg tgtgacgttg tagcgacact agcagtaagt
     2341 atttctgcta aatcacagac gcacatttcc ttataggttt ctaaagcata gaaaatttta
     2401 attctagaaa aatcgctgaa acatttccct aacacaagta agtttgaaaa atctttttga
     2461 tccaatttat tttttattcc atttactttt tctttatgta cgacagtcac ttggcaaact
     2521 tttttcattc atctcacctc acattcaaac gattatttga atgaagtata cctatttgat
     2581 cactcttttg tcaatctacc ttaattccaa tacactatta taagcaattt catgtattga
     2641 agtaaactta cttgcaagtg cgtttttgat tccaagttaa aaacatatta ttattctaca
     2701 taatttccta atcaaat
//
LOCUS       DACEMS010000277          965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.250, whole genome shotgun sequence.
ACCESSION   DACEMS010000277 DACEMS010000000
VERSION     DACEMS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.250"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..663
                     /locus_tag="IW735_002847"
     CDS             <1..663
                     /locus_tag="IW735_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HAQ1045775.1"
                     /translation="IEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIA
                     SFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNAT
                     TVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGT
                     NFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTY
                     KD"
     gene            complement(793..>965)
                     /locus_tag="IW735_002848"
     CDS             complement(793..>965)
                     /locus_tag="IW735_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1045776.1"
                     /translation="KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEW
                     IYSSRKYINFDK"
BASE COUNT          383 a           86 c          165 g          331 t
ORIGIN      
        1 attgaatatt cgtatattag tgatgaatta tctatactag gttataaaga aattaaagga
       61 acttttttaa caccagaaat ttattctact atgtcagaag aagaacaaaa tttgttaaaa
      121 cgagatattg ccagtttttt aagacaaatg cacggtttag attatacaga tattagtgaa
      181 tgtactattg ataataaaca aaatgtatta gaagagtata tattgttgcg tgaaactatt
      241 tataatgatt taactgatat agaaaaagat tatatagaaa gttttatgga aagactaaat
      301 gcaacaacag tttttgaggg taaaaagtgt ttatgccata atgattttag ttgtaatcat
      361 ctattgttag atggcaataa tagattaact ggaataattg attttggaga ttctggaatt
      421 atagatgaat attgtgattt tatatactta cttgaagata gtgaagaaga aataggaaca
      481 aattttggag aagatatatt aagaatgtat ggaaatatag atattgagaa agcaaaagaa
      541 tatcaagata tagttgaaga atattatcct attgaaacta ttgtttatgg aattaaaaat
      601 attaaacagg aatttatcga aaatggtaga aaagaaattt ataaaaggac ttataaagat
      661 tgattatata atatatgaaa agctattata aaagacatta gtattaaata gtttaaaaaa
      721 atgaaaaata ataaaggaag tgagtcaagt ccagactcct gtgtaaaatg ctatacaatg
      781 tttttaccat ttctacttat caaaattgat gtattttctt gaagaataaa tccattcatc
      841 atgtaggtcc ataagaacgg ctccaattaa gcgattggct gatgtttgat tggggaagat
      901 gcgaataatc ttttctcttc tgcgtacttc ttgattcagt cgttcaatta gattggtact
      961 cttta
//
LOCUS       DACEMS010000278          957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.251, whole genome shotgun sequence.
ACCESSION   DACEMS010000278 DACEMS010000000
VERSION     DACEMS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.251"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            344..892
                     /gene="aac(6')"
                     /locus_tag="IW735_002849"
     CDS             344..892
                     /gene="aac(6')"
                     /locus_tag="IW735_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1045777.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          339 a          138 c          191 g          289 t
ORIGIN      
        1 gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat
       61 tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga
      121 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
      181 ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta agataaataa
      241 tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt
      301 tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt
      361 tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc
      421 ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat
      481 cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata
      541 cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca
      601 aataggtact cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac
      661 gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta
      721 tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt
      781 ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa
      841 accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga
      901 ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa attactt
//
LOCUS       DACEMS010000279          704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E471
            SAMN07141526-rid9870323.guided.265, whole genome shotgun sequence.
ACCESSION   DACEMS010000279 DACEMS010000000
VERSION     DACEMS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141526
            Sequence Read Archive: SRR5811449
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740779.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:36:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,817
            Genes (coding)                    :: 2,743
            CDSs (with protein)               :: 2,743
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141526-rid9870323.guided.265"
                     /isolate="E471"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2011"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>704
                     /locus_tag="IW735_002850"
     CDS             <1..>704
                     /locus_tag="IW735_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1045778.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYI"
BASE COUNT          295 a           63 c          115 g          231 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat atta
//
